Materials & Streams Report October 6, 2020

for 6-Citric Acid Simulation

1. OVERALL PROCESS DATA

Annual Operating Time 6,790.82h

Recipe Batch Time 6,790.82h
Recipe Cycle Time 6,790.82h
Number of Batches per Year 1.00
MP = Undefined

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2.1 STARTING MATERIAL REQUIREMENTS (per Section)

Amount
Gross
Needed Molar Mass
Mass
Section Starting Material Active Product (kg Yield Yield
Yield
Sin/kg (%) (%)
(%)
MP)
Main Section (none) (none) Unknown Unknown Unknown Unknown
Sin = Section Starting Material, Aout = Section Active Product

2.2 BULK MATERIALS (Entire Process)

Material kg/yr kg/batch kg/kg MP

Air 806,512 806,512.38
Amm. Nitrate 6,731 6,731.22
Biomass 187 187.11
Glucose 410,610 410,609.51
HCl 27,234 27,234.25
KH2PO4 512 512.35
magnesium 13,617 13,617.12
Nitrogen 610,975 610,974.74
Oxygen 162,411 162,411.01
Potassium 13,617 13,617.12
Sodium Hydroxid 51,621,692 51,621,692.08
Water 4,471,127 4,471,126.93
TOTAL 58,145,226 58,145,225.82

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2.3 BULK MATERIALS (per Section)

SECTIONS IN: Main Branch

Main Section
Material kg/yr kg/batch kg/kg MP
Air 806,512 806,512.38
Amm. Nitrate 6,731 6,731.22
Biomass 187 187.11
Glucose 410,610 410,609.51
HCl 27,234 27,234.25
KH2PO4 512 512.35
magnesium 13,617 13,617.12
Nitrogen 610,975 610,974.74
Oxygen 162,411 162,411.01
Potassium 13,617 13,617.12
Sodium Hydroxid 51,621,692 51,621,692.08
Water 4,471,127 4,471,126.93
TOTAL 58,145,226 58,145,225.82

2.4 BULK MATERIALS (per Material)

Air
Procedure % Total kg/yr kg/batch kg/kg MP
Main Section (Main Branch)
P-12 100.00 806,512 806,512.38
TOTAL 100.00 806,512 806,512.38

Amm. Nitrate
Procedure % Total kg/yr kg/batch kg/kg MP
Main Section (Main Branch)
P-1 6.27 422 422.04
P-11 93.73 6,309 6,309.18
TOTAL 100.00 6,731 6,731.22

Biomass
Procedure % Total kg/yr kg/batch kg/kg MP
Main Section (Main Branch)
P-1 100.00 187 187.11
TOTAL 100.00 187 187.11

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Glucose
Procedure % Total kg/yr kg/batch kg/kg MP
Main Section (Main Branch)
P-1 6.27 25,745 25,744.83
P-11 93.73 384,865 384,864.68
TOTAL 100.00 410,610 410,609.51

HCl
Procedure % Total kg/yr kg/batch kg/kg MP
Main Section (Main Branch)
P-5 100.00 27,234 27,234.25
TOTAL 100.00 27,234 27,234.25

KH2PO4
Procedure % Total kg/yr kg/batch kg/kg MP
Main Section (Main Branch)
P-1 6.10 31 31.26
P-11 93.90 481 481.09
TOTAL 100.00 512 512.35

magnesium
Procedure % Total kg/yr kg/batch kg/kg MP
Main Section (Main Branch)
P-5 100.00 13,617 13,617.12
TOTAL 100.00 13,617 13,617.12

Nitrogen
Procedure % Total kg/yr kg/batch kg/kg MP
Main Section (Main Branch)
P-1 6.27 38,307 38,307.43
P-2 93.73 572,667 572,667.31
TOTAL 100.00 610,975 610,974.74

Oxygen
Procedure % Total kg/yr kg/batch kg/kg MP
Main Section (Main Branch)
P-1 6.27 10,183 10,182.99
P-2 93.73 152,228 152,228.02
TOTAL 100.00 162,411 162,411.01

Potassium
Procedure % Total kg/yr kg/batch kg/kg MP
Main Section (Main Branch)
P-5 100.00 13,617 13,617.12
TOTAL 100.00 13,617 13,617.12

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Sodium Hydroxid
Procedure % Total kg/yr kg/batch kg/kg MP
Main Section (Main Branch)
P-6 100.00 51,621,692 51,621,692.08
TOTAL 100.00 51,621,692 51,621,692.08

Water
Procedure % Total kg/yr kg/batch kg/kg MP
Main Section (Main Branch)
P-1 6.28 280,732 280,732.12
P-3 0.00 100 100.00
P-10 0.00 100 100.00
P-11 93.72 4,190,195 4,190,194.81
TOTAL 100.00 4,471,127 4,471,126.93

2.5 BULK MATERIALS: SECTION TOTALS (kg/batch)

Raw Material Main Section

Air 806,512.38
Amm. Nitrate 6,731.22
Biomass 187.11
Glucose 410,609.51
HCl 27,234.25
KH2PO4 512.35
magnesium 13,617.12
Nitrogen 610,974.74
Oxygen 162,411.01
Potassium 13,617.12
Sodium Hydroxid 51,621,692.08
Water 4,471,126.93
TOTAL 58,145,225.82

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2.6 BULK MATERIALS: SECTION TOTALS (kg/yr)

Raw Material Main Section

Air 806,512
Amm. Nitrate 6,731
Biomass 187
Glucose 410,610
HCl 27,234
KH2PO4 512
magnesium 13,617
Nitrogen 610,975
Oxygen 162,411
Potassium 13,617
Sodium Hydroxid 51,621,692
Water 4,471,127
TOTAL 58,145,226

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3. STREAM DETAILS

Stream Name S-128 S-129 S-134 S-135

Source INPUT INPUT INPUT INPUT
Destination P-11 P-11 P-11 P-11
Stream Properties
Activity (U/ml) 0.00 0.00 0.00 0.00
Temperature (°C) 25.00 25.00 25.00 25.00
Pressure (bar) 1.01 1.01 1.01 1.01
Density (g/L) 1,173.66 1,725.00 1,636.00 994.70
Total Enthalpy (kW-h) 5,308.79 82.02 2.46 122,278.77
Specific Enthalpy (kcal/kg) 11.87 11.19 4.39 25.11
Heat Capacity (kcal/kg-°C) 0.47 0.45 0.18 1.00
Component Flowrates (kg/batch)
Amm. Nitrate 0.00 6,309.18 0.00 0.00
Glucose 384,864.68 0.00 0.00 0.00
KH2PO4 0.00 0.00 481.09 0.00
Water 0.00 0.00 0.00 4,190,194.81
TOTAL (kg/batch) 384,864.68 6,309.18 481.09 4,190,194.81
TOTAL (L/batch) 327,918.64 3,657.50 294.06 4,212,502.86

Stream Name S-136 S-106 S-101 S-102

Source P-11 P-14 INPUT INPUT
Destination P-14 P-2 P-1 P-1
Stream Properties
Activity (U/ml) 0.00 0.00 0.00 0.00
Temperature (°C) 25.00 35.00 25.00 25.00
Pressure (bar) 10.13 10.13 1.01 1.01
Density (g/L) 1,008.25 1,004.37 1,010.67 1,008.25
Total Enthalpy (kW-h) 127,672.04 178,492.53 18.35 8,540.39
Specific Enthalpy (kcal/kg) 23.98 33.52 25.32 23.98
Heat Capacity (kcal/kg-°C) 0.96 0.95 1.01 0.96
Component Flowrates (kg/batch)
Amm. Nitrate 6,309.18 6,309.18 0.00 422.04
Biomass 0.00 0.00 187.11 0.00
Glucose 384,864.68 384,864.68 0.00 25,744.83
KH2PO4 481.09 481.09 0.00 31.26
Water 4,190,194.81 4,190,194.81 436.60 280,295.52
TOTAL (kg/batch) 4,581,849.76 4,581,849.76 623.71 306,493.65
TOTAL (L/batch) 4,544,373.05 4,561,914.29 617.12 303,987.07

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Stream Name S-103 S-105 S-104 S-107
Source INPUT P-1 P-1 INPUT
Destination P-1 OUTPUT P-2 P-2
Stream Properties
Activity (U/ml) 0.00 0.00 0.00 0.00
Temperature (°C) 25.00 37.00 37.00 25.00
Pressure (bar) 1.01 1.01 1.01 1.01
Density (g/L) 1.18 1.18 1,013.28 1.18
Total Enthalpy (kW-h) 341.91 1,062.86 12,772.91 5,111.34
Specific Enthalpy (kcal/kg) 6.07 19.16 35.65 6.07
Heat Capacity (kcal/kg-°C) 0.24 0.24 0.96 0.24
Component Flowrates (kg/batch)
Biomass 0.00 0.00 2,984.10 0.00
Carb. Dioxide 0.00 9,041.01 0.00 0.00
Glucose 0.00 0.00 1.99 0.00
KH2PO4 0.00 0.00 0.15 0.00
mCA 0.00 0.00 18,709.91 0.00
Nitrogen 38,307.43 38,578.69 0.00 572,667.31
Oxygen 10,182.99 105.13 0.00 152,228.02
Potassium 0.00 0.00 8.94 0.00
Water 0.00 0.00 286,533.81 0.00
TOTAL (kg/batch) 48,490.42 47,724.83 308,238.91 724,895.33
TOTAL (L/batch) 41,241,410.64 40,360,667.67 304,198.23 616,528,101.75

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Stream Name S-108 S-109 S-110 S-111
Source P-2 P-2 INPUT P-3
Destination OUTPUT P-3 P-3 OUTPUT
Stream Properties
Activity (U/ml) 0.00 0.00 0.00 0.00
Temperature (°C) 37.00 37.00 25.00 37.00
Pressure (bar) 1.01 1.01 1.01 1.01
Density (g/L) 1.16 1,010.10 994.70 1,033.90
Total Enthalpy (kW-h) 12,998.83 202,650.67 2.92 2,138.63
Specific Enthalpy (kcal/kg) 15.54 35.58 25.11 37.17
Heat Capacity (kcal/kg-°C) 0.24 0.96 1.00 1.00
Component Flowrates (kg/batch)
Amm. Nitrate 0.00 2,208.21 0.00 8.72
Biomass 0.00 30,162.41 0.00 30,162.41
Carb. Dioxide 87,843.16 0.00 0.00 0.00
Glucose 0.00 134,724.05 0.00 532.11
KH2PO4 0.00 178.94 0.00 0.71
mCA 0.00 200,513.74 0.00 791.96
Nitrogen 577,021.82 0.00 0.00 0.00
Oxygen 54,848.92 0.00 0.00 0.00
Potassium 0.00 95.80 0.00 0.38
Water 0.00 4,533,103.57 100.00 18,005.73
TOTAL (kg/batch) 719,713.91 4,900,986.73 100.00 49,502.02
TOTAL (L/batch) 618,646,942.61 4,851,980.92 100.53 47,878.81

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Stream Name S-112 S-114 S-113 S-115
Source P-3 P-4 P-4 INPUT
Destination P-4 P-5 OUTPUT P-5
Stream Properties
Activity (U/ml) 0.00 0.00 0.00 0.00
Temperature (°C) 37.00 37.09 37.09 25.00
Pressure (bar) 1.01 1.01 1.01 1.01
Density (g/L) 1,009.86 1,015.32 990.30 2.98
Total Enthalpy (kW-h) 200,506.86 155,891.94 45,114.95 3,017.38
Specific Enthalpy (kcal/kg) 35.56 35.23 37.19 47.66
Heat Capacity (kcal/kg-°C) 0.96 0.95 1.00 0.40
Component Flowrates (kg/batch)
Amm. Nitrate 2,199.49 2,199.49 0.00 0.00
Glucose 134,191.94 134,191.94 0.00 0.00
HCl 0.00 0.00 0.00 27,234.25
KH2PO4 178.23 178.23 0.00 0.00
magnesium 0.00 0.00 0.00 13,617.12
mCA 199,721.78 199,721.78 0.00 0.00
Potassium 95.42 95.42 0.00 13,617.12
Water 4,515,197.84 3,471,405.56 1,043,792.28 0.00
TOTAL (kg/batch) 4,851,584.71 3,807,792.43 1,043,792.28 54,468.49
TOTAL (L/batch) 4,804,199.88 3,750,351.02 1,054,019.23 18,304,050.00

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Stream Name S-116 S-117 S-118 S-120
Source P-5 P-5 INPUT P-6
Destination OUTPUT P-6 P-6 P-7
Stream Properties
Activity (U/ml) 0.00 0.00 0.00 0.00
Temperature (°C) 25.01 37.09 25.00 37.09
Pressure (bar) 1.01 1.01 1.01 1.01
Density (g/L) 2.98 1,015.34 1,913.35 1,089.52
Total Enthalpy (kW-h) 3,017.38 155,756.90 781,354.96 35,830.09
Specific Enthalpy (kcal/kg) 47.66 35.22 13.02 29.93
Heat Capacity (kcal/kg-°C) 0.40 0.95 0.52 0.80
Component Flowrates (kg/batch)
Amm. Nitrate 0.00 2,199.49 0.00 2,199.49
Glucose 0.00 134,191.94 0.00 134,191.94
HCl 27,234.25 0.00 0.00 0.00
KH2PO4 0.00 178.23 0.00 178.23
magnesium 13,617.12 0.00 0.00 0.00
mCA 0.00 199,721.78 0.00 199,621.92
Potassium 13,617.12 95.42 0.00 95.42
Sodium Hydroxid 0.00 0.00 51,621,692.08 0.00
Water 0.00 3,468,281.30 0.00 693,656.26
TOTAL (kg/batch) 54,468.49 3,804,668.16 51,621,692.08 1,029,943.26
TOTAL (L/batch) 18,304,663.01 3,747,196.14 26,979,812.24 945,321.55

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Stream Name S-119 S-121 S-122 S-125
Source P-6 P-7 P-8 P-8
Destination OUTPUT P-8 P-9 P-10
Stream Properties
Activity (U/ml) 0.00 0.00 0.00 0.00
Temperature (°C) 26.13 37.09 181.42 25.00
Pressure (bar) 1.01 10.54 10.54 10.54
Density (g/L) 1,826.77 1,089.52 5.03 1,103.41
Total Enthalpy (kW-h) 901,281.77 35,828.33 55,887.82 22,334.24
Specific Enthalpy (kcal/kg) 14.26 29.93 693.24 20.01
Heat Capacity (kcal/kg-°C) 0.55 0.80 0.46 0.80
Component Flowrates (kg/batch)
Amm. Nitrate 0.00 2,199.49 0.00 2,199.49
citric acid cry 0.00 0.00 0.00 179,659.73
Glucose 0.00 134,191.94 0.00 134,191.94
KH2PO4 0.00 178.23 0.00 178.23
mCA 99.86 199,621.92 0.00 19,962.19
Potassium 0.00 95.42 0.00 95.42
Sodium Hydroxid 51,621,692.08 0.00 0.00 0.00
Water 2,774,625.04 693,656.26 69,365.63 624,290.63
TOTAL (kg/batch) 54,396,416.98 1,029,943.26 69,365.63 960,577.64
TOTAL (L/batch) 29,777,369.58 945,320.79 13,802,577.51 870,555.12

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Stream Name S-126 S-130 S-127 S-131
Source INPUT P-10 P-10 INPUT
Destination P-10 P-12 OUTPUT P-12
Stream Properties
Activity (U/ml) 0.00 0.00 0.00 0.00
Temperature (°C) 25.00 25.00 25.00 25.00
Pressure (bar) 1.01 1.01 1.01 1.01
Density (g/L) 994.70 1,333.37 1,012.41 1.18
Total Enthalpy (kW-h) 2.92 4,696.81 17,640.35 5,670.58
Specific Enthalpy (kcal/kg) 25.11 12.28 24.03 6.05
Heat Capacity (kcal/kg-°C) 1.00 0.49 0.96 0.24
Component Flowrates (kg/batch)
Amm. Nitrate 0.00 1,687.44 512.05 0.00
citric acid cry 0.00 167,802.19 11,857.54 0.00
Glucose 0.00 102,951.75 31,240.19 0.00
KH2PO4 0.00 136.74 41.49 0.00
mCA 0.00 15,314.95 4,647.24 0.00
Nitrogen 0.00 0.00 0.00 618,690.06
Oxygen 0.00 0.00 0.00 187,822.32
Potassium 0.00 73.21 22.21 0.00
Water 100.00 41,138.39 583,252.24 0.00
TOTAL (kg/batch) 100.00 329,104.66 631,572.97 806,512.38
TOTAL (L/batch) 100.53 246,822.55 623,833.10 683,936,225.82

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Stream Name S-132 S-133 S-124 S-123
Source P-12 P-12 P-9 P-9
Destination OUTPUT OUTPUT OUTPUT OUTPUT
Stream Properties
Activity (U/ml) 0.00 0.00 0.00 0.00
Temperature (°C) 70.00 70.00 20.00 20.00
Pressure (bar) 1.01 1.01 1.01 1.01
Density (g/L) 1.11 1,522.03 0.75 996.53
Total Enthalpy (kW-h) 85,059.88 5,306.04 24,986.48 810.43
Specific Enthalpy (kcal/kg) 75.62 27.21 619.87 20.11
Heat Capacity (kcal/kg-°C) 0.26 0.39 0.45 1.00
Component Flowrates (kg/batch)
Amm. Nitrate 1,687.44 0.00 0.00 0.00
citric acid cry 0.00 167,802.19 0.00 0.00
Glucose 102,951.75 0.00 0.00 0.00
KH2PO4 136.74 0.00 0.00 0.00
mCA 15,314.95 0.00 0.00 0.00
Nitrogen 618,690.06 0.00 0.00 0.00
Oxygen 187,822.32 0.00 0.00 0.00
Potassium 73.21 0.00 0.00 0.00
Water 41,138.39 0.00 34,682.81 34,682.81
TOTAL (kg/batch) 967,814.85 167,802.19 34,682.81 34,682.81
TOTAL (L/batch) 870,280,960.41 110,249.22 46,311,009.50 34,803.69

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4. OVERALL COMPONENT BALANCE (kg/batch)

COMPONENT INITIAL INPUT OUTPUT FINAL IN-OUT

Amm. Nitrate 0.00 6,731.22 2,208.21 0.00 4,523.01
Biomass 0.00 187.11 30,162.41 0.00 - 29,975.30
Carb. Dioxide 0.00 0.00 96,884.17 191.89 - 97,076.06
citric acid cry 0.00 0.00 179,659.73 0.00 - 179,659.73
Glucose 0.00 410,609.51 134,724.05 0.00 275,885.46
HCl 0.00 27,234.25 27,234.25 0.00 0.00
KH2PO4 0.00 512.35 178.94 0.00 333.41
magnesium 0.00 13,617.12 13,617.12 0.00 0.00
mCA 0.00 0.00 20,854.01 0.00 - 20,854.01
Nitrogen 11,364.57 1,229,664.80 1,234,290.57 7,003.33 - 264.53
Oxygen 3,450.06 350,233.32 242,776.37 1,864.70 109,042.31
Potassium 0.00 13,617.12 13,712.92 0.00 - 95.80
Sodium Hydroxid 0.00 51,621,692.08 51,621,692.08 0.00 0.00
Water 0.00 4,471,126.93 4,530,179.31 3,124.27 - 62,176.65
TOTAL 14,814.63 58,145,225.82 58,148,174.15 12,184.19 317.89
Overall Error: 0.001%

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5. EQUIPMENT CONTENTS

SFR-101
Procedure Operation Time (in h) Volume (in L)
P-1 START 0.00 0.00
P-1 CHARGE-1 (Charge) 1,408.27 77.14
P-1 CHARGE-2 (Charge) 1,472.46 38,075.52
FERMENT-1 (Batch Stoich.
P-1 1,544.46 38,024.78
Fermentation)
P-1 TRANSFER-OUT-1 (Transfer Out) 1,608.93 0.00

FR-101
Procedure Operation Time (in h) Volume (in L)
P-2 START 0.00 0.00
P-2 TRANSFER-IN-1 (Transfer In) 5,963.71 38,023.74
P-2 TRANSFER-IN-2 (Transfer In) 6,282.22 608,264.26
FERMENT-1 (Batch Stoich.
P-2 6,450.22 606,497.62
Fermentation)
P-2 TRANSFER-OUT-1 (Transfer Out) 6,790.82 0.00

INX-101
Procedure Operation Time (in h) Volume (in L)
P-5 START 0.00 0.00
P-5 LOAD-1 (INX Column Loading) 412.20 630.98
P-5 WASH-1 (INX Column Washing) 425.21 630.98

GAC-101
Procedure Operation Time (in h) Volume (in L)
P-6 START 0.00 0.00
P-6 LOAD-1 (GA Column Loading) 68.00 280,187.46
P-6 WASH-1 (GA Column Washing) 70.00 0.00

V-101
Procedure Operation Time (in h) Volume (in L)
P-7 START 0.00 0.00
P-7 TRANSFER-IN-1 (Transfer In) 1,032.19 189,064.16
P-7 TRANSFER-OUT-1 (Transfer Out) 1,146.88 0.00

V-102
Procedure Operation Time (in h) Volume (in L)
P-11 START 0.00 0.00
P-11 CHARGE-1 (Charge) 3,608.59 20,494.91
P-11 CHARGE-2 (Charge) 3,609.01 20,723.51
P-11 CHARGE-3 (Charge) 3,609.27 20,741.89
P-11 CHARGE-4 (Charge) 3,718.64 284,023.32
P-11 TRANSFER-OUT-1 (Transfer Out) 3,838.21 0.00

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