PERALES, DANIEL JOHN B.

ASSIGNMENT 1
2DMT MT6310

A. Draw the ff peptides:

1. HI

9.17

2.36

6.0

2. YES
4.25

9.11

2.21

10.07

3. CHEM

6.0

10.78

2.28

8.33

4.25
 4. PRAISE 12.48

4.25

10.6

2.19

B. What is the pI of each peptide?

1. HI

-H+ -H+ -H+

+2 +1 0 -1
2.36 6.0 9.17
𝟔.𝟎+𝟗.𝟏𝟕
𝒑𝑰 = 𝒑𝑰 = 𝟕. 𝟓𝟖𝟓 ≈ 𝟕. 𝟔
𝟐

2. YES

-H+ -H+ -H+ -H+

+1 0 -1 -2 -3
2.21 4.25 9.11 10.07
𝟐.𝟐𝟏+𝟒.𝟐𝟓
𝒑𝑰 = 𝒑𝑰 = 𝟑. 𝟐𝟑
𝟐

3. CHEM

-H+ -H+ -H+ -H+ -H+

+2 +1 0 -1 -2 -3
2.28 4.25 6.0 8.33 10.78
𝟒.𝟐𝟓+𝟔.𝟎
𝒑𝑰 = 𝒑𝑰 = 𝟓. 𝟏𝟐𝟓 ≈ 𝟓. 𝟏
𝟐
 4. PRAISE

-H+ -H+ -H+ -H+

+2 +1 0 -1 -2
2.19 4.25 10.6 12.48
𝟒.𝟐𝟓+𝟏𝟎.𝟔
𝒑𝑰 = 𝒑𝑰 = 𝟕. 𝟒𝟐𝟓 ≈ 𝟕. 𝟒𝟑
𝟐

C. Predict the ionic form of the peptide (given above) that will predominate at:

pH 1.00
1. HI (+2 net charge)

2. YES (+1 net charge)

3. CHEM (+2 net charge)

 4. PRAISE (+2 net charge)

pH 7.00

1. HI (0 net charge)

2. YES (-1 net charge)

3. CHEM (-1 net charge)

 4. PRAISE (0 net charge)

pH 13.00

1. HI (-1 net charge)

2. YES (-3 net charge)

3. CHEM (-3 net charge)

4. PRAISE (-2 net charge)