Francisco, Frances Lorraine R.

Professor: Al Hadad Kalon

BLOOD COMPONENTS (E-LEARNING ACTIVITY)

COMPONENT SHELF LIFE STORAGE QUALITY VOLUME INDICATIONS CONTENT DOSAGE TRANSFUSION
TEMPERATURE CONTROL FOR USE CRITERIA
Whole blood CPD-21 d 1–6°C Hct approx. 450–500 mL Volume RBC ↑hgb 1g/dL ABO, Rh
CPDA-1 35 d 40% expansion, Plasma ↑hct 3%
CP2D-21 d ↑O2 Platelets WBCs
ACD-21 d
Whole blood Original 1–6°C 25 Gy to center 450–500 mL Prevent GVHD RBC ↑hgb 1g/dL ABO, Rh
irradiated expiration or of canister volume Plasma ↑hct 3%
28 days from expansion ↑O2 Platelets
irradiation
RBCs CPD-21 d 1–6°C Hct ≤ 80% 250–300 mL ↑O2 RBC ↑hgb 1g/dL ABO, Rh
CPDA-1 35 d ↑hct 3%
CP2D-21 d
ACD-21 d AS-
42 d
RBC aliquots CPDA-1 35 d 1–6°C Hct ≤ 80% varies ↑O2 RBC 10 mL/kg ↑hgb 2 ABO, Rh
(closed g/dL
system)
RBC irradiated Original 1–6°C 25 Gy to center 250–300 mL Prevent GVHD RBC ↑hgb 1 g/dL ABO, Rh
outdate or of canister ↑O2 ↑hct 3%
28 days from
irradiation
RBC Closed 1–6°C < 5 × 106 WBCs 250–300 mL Febrile rxn, RBC Few ↑hgb 1 g/dL ABO, Rh
leukoreduced system: ≥ 85% RBC ↑O2 platelets; ↑hct 3%
same Open recovery residual plasma
system: 24
hours
Washed RBCs 24 hours 1–6°C Hct 70–80% 180 mL IgA-negative RBC WBC < 5 × ↑hgb 1 g/dL ABO, Rh
persons PNH 108 ↑3%
Frozen RBCs 10 years ≤ –65°C Rare RBC Glycerol
phenotypes
RBC 24 hr 1–6°C 80% RBC 180 mL Rare RBC Saline ↑hgb 1 g/dL ABO, Rh
deglycerolized recovery < 1% phenotypes Dextrose < 1% ↑hct 3%
glycerol < 300 ↑O2 WBC, platelets
mg hgb
Platelets, RD 5d 20–24°C ≥ 5.5 × 1010plts 50–70 mL Thrombocytope Platelets ↑5k–10k/µL
 pH ≥ 6.2 nia DIC,
bleeding
Platelets, SD 5d 20–24°C ≥ 3 × 1011 pH ≥ 200–400 mL Platelet Platelets ↑30k–60k/µL HLA compatible
6.2 refractoriness
Platelets, 5d 20–24°C 25 Gy to center Same Prevent GVHD Platelets Same Same
irradiated of canister

Platelets, 4 hr 20–24°C pH ≥ 6.2 Varies Thrombocytope Platelets Varies SD: HLA

pooled nia DIC,
bleeding
Platelets, 5d 20–24°C < 5 × 106SD < pH≥ 6.2 Febrile rxns Platelets RD: ↑5–10k SD: ABO
leukoreduced 8.3 × 105RD ↑30–60k
↑Factor 20–30%
10–20 mL/kg
FFP 1 yr 7 yr –18°C –65°C 8 hr CPD, CPDA- 200–250 mL Coagulation 1 U/mL clotting RD: ↑5–10k SD: Same
1, CP2D 6 hr deficiency Liver factors ↑30–60k
ACD disease DIC ↑Factor 20–30%
Massive trx 10–20 mL/kg
PF24 1 yr 7 yr –18°C –65°C 24 hr WB 150–250 mL Same ↓Labile factors Same Same
SDP 1–6°C liquid 5 days after WB Frozen 6 hr 150–250 mL Stabile clotting Same ABO
expiration factors
Frozen: 5 yr
Cryoprecipitate Frozen: 1 yr –18°C 20–24°C FVIII:C 80 IU 10–25 mL Hemophilia A FVIII:C (80– ↑Fibrinogen 5– Reconstitute
Thawed: 6 hr VWD FXIII 120U) VWF (40– 10 mg/dL before infusion
Pooled: 4 hr deficiency 70%) FXIII (20–
Fibrin sealant 30%) Fibrinogen
Hypofibrinogen (150 mg/dL)
emia
FVII Check vial 1–6°C 10–30 mL Hemophilia A FVIII Trace 1U FVIII/kg body Reconstitute
concentrates other clotting wt ↑2% before infusion
factors
FIX Check Vial 1–6°C 20–30 mL Hemophilia B FVIII Trace 1U FIX/kg body HLA compatible
concentrates other clotting wt ↑1.5%
factors
Granulocytes 24 hr 20–24°C ≥ 1.0 × 1010 200–600 mL Neutropenia < WBC RBC Plts 1–2 × 1010/ ABO, Rh, HLA
500 PMN/uL plasma infusion four
daily doses
Granulocytes, 24 hr 20–24°C ≥ 1.0 × 1010 200–600 mL Prevent GVHD Same Same Same
irradiated neutropenia
 ISG 3 yr IM Varies Prophylaxis Gamma IM or IV
1 yr IV Immunodeficie globulins IgG,
ncy IgM, IgA
Hypogammaglo
bulinemia
NSA 5 yr, 25% 2–10°C 50 mL Plasma volume 96% albumin
250 mL expansion 4% globulin
PPF, 5% 5 yr 2–10°C 250 Plasma volume Albumin 80–
expansion 85% Globulin
15–20%
Dextran 6% (dex 40) Volume
10% (dex 70) expansion
6% burns
HES 2 yr Volume
expansion
burns
RhIg 24 hr 1–6°C 1 mL Rh HDN Anti-D IgG 300 µg 120 µg 50 Mother Rh
µg negative; baby
Rh-positive, Rh-
unknown