Analysis of Variance- Two way classification:

When two independent factors might affect the variable of

interest it is possible to design a test so that an analysis of
variance can be used to test the effects of these two factors
simultaneously. Such a test is called a two-way classification of
analysis of variance or a two factor analysis of variance.
Source of Sum of Degrees of F-ratio
variation squares freedom Mean square
Between SSC
columns V1=K-1 MSC  MSC
(k=no. of SSC K 1 Fc 
MSE
columns)
Between SSR
rows SSR V2=r-1 MSR 
r 1
(r=no. of MSR
rows) FR 
MSE
Residual SSE
MSE
(or) SSE V3=(K-)(r-1) (K 1)(r 1)
Error

Problem:
Three breed of cattle A, B and C were fed by 4 different rations P, Q,
R and S. The following table gives the gains in weight. Test whether
there is any significant difference between breeds and rations at 5%
level of significance.
Rations
P Q R S
A 6 3 2 9
Breeds B 1 3 8 9
C 7 3 5 2
Dr.K.Kavitha
 Breeds Rations
P Q R S Total
A 6 3 2 9 20
B 1 3 8 7 19
C 7 3 5 2 17
Total 14 9 15 18 56(T)

Null Hypothesis Ho:

(i) There is no significant difference between rations.
(ii) There is no significant difference between breeds.
Step 1:
Grand total (T):56
Step 2:
2
(56) 2
Correction factor (C.F)= T   261.33
N 12

Step 3:
SSC= Sum of squares between columns (Rations)

(14)2 (9)2 (15)2 (18) 2

     C.F
3 3 3 3
 14

d.f=v1=k-1=4-1=3
Step 4:
SSR=Sum of squares between rows (Breeds)
2
(19) 2 (17)2
= (20)    C.F  1.17
4 4 4

d.f=v2=r-1=3-1=2
Dr.K.Kavitha
Step 5:
TSS=Total sum of squares
=Sum of squares of each values-C.F
 (6) 2  (3) 2  (2) 2  .........(2) 2  C.F
 78.67

Step 6:
SSE=Residual
=Total sum of squares-[sum of squares between columns (or)
Rations+ Sum of squares between rows (or) Breeds]
=TSS-(SSC+SSR)
=78.67-(14+1.17)
=63.5
Step 7:
Degrees of freedom for residual v3=(k-1)(r-1)=3 x 2=6
Source of Sum of Degrees of F-ratio
variation squares freedom Mean square
Between SSC
columns V1=K-1=3 MSC  MSE
(k=no. of SSC=14 K 1 Fc 
=4.6 MSC
columns) =2.3
Between SSR
rows SSR=1.17 V2=r-1=2 MSR 
r 1
(r=no. of MSE
=0.585 FR 
rows) MSR
Residual SSE =18.08
V3=(K-1)(r-1)=6 MSE
(or) SSE=63.5 (K 1)(r 1)
Error =10.58
Dr.K.Kavitha
Conclusion:
(i) Table of value of F for (6,3) d.f at 5% L.O.S is 8.94.
Fc<Ft
Accept the null hypothesis Ho
i.e.,There is no significant difference between rations.

(ii) Table of value of F for (6,2) d.f at 5% L.O.S is 19.3.

FR<Ft
Accept the null hypothesis Ho
i.e., There is no significant difference between breeds.

Dr.K.Kavitha