Practical Research 2

The Potential Of Mayana (Coleus

Blumei) Leaves as an Organic
Pigment for Liptint, Markers, and
Ballpen Ink
RESEARCHERS Kent Vincent Francia
1

Aizah Ampaso
Khyle Andrei Buena
Katrina Mae Urlanda
Joanna Baldago
Claire Nacario
Katrice Eunice Bagacina
Ehrica Belmonte
Walter Bacsain
INTRODUCTION The Philippines, with its unique geography and isolation, boasts diverse ecosystems,
contributing to a rich array of plant life, including endemic species. This insight is drawn
from the work of Agape et al. (2018). Mangroves, such as bakawan and bakhaw, play a
crucial role in coastal protection and marine species nurseries, showcasing the ecological
significance of diverse habitats.
The Sierra Madre and Cordillera mountain ranges harbor diverse plant species, utilized
by native tribes for various purposes, adding to the nation’s natural beauty. The rich
plant life in the Philippines supports traditional medicine, agriculture, and herbal goods
production, offering ecological and commercial advantages. Leaves, flowers, fruits, and
roots of plants serve as pigment sources for environmentally friendly inks. Plant-based
inks offer sustainability, safety, and adaptability, presenting a viable alternative to
synthetic inks (Abdon et al., 2021³). The research explores using Mayana (Coleus blumei)
as an alternative pigment for synthetic ingredients in lip tint, markers, and ballpen ink,
aiming to reduce environmental impacts, health risks, and production costs successful
implementation of Mayana pigment could reduce reliance on synthetic pigments,
offering a natural sustainable alternative beneficial to both manufacturers and
consumers.
 This study entitled “The Potential of Mayana (Coleus blumei) leaves extract as an Organic Pigment
STATEMENT OF THE for Lip Tint, Marker, and Ballpen ink” seeks to answer the following questions. Specifically, it will
answer the following specific questions:
1.What effect do the various Mayana leaf pigment amounts have on the longevity of ballpoint ink,
markers, and lip tint along:
a.50g
PROBLEM
b.80g
c.100g

2.How effective is Mayana leaf extract as an organic pigment for lip tint, markers, and ballpoint
ink concerning the following attributes?”
a. Pigmentation
b. Fragrance
c. Absorption
d. Fast drying

3.Is there a significant difference between Mayana (Coleus blumei) and the standard or
commercial ink in terms of pigmentation, fragrance, absorption and fast drying?

4.Is the amount spent on Mayana pigment extraction cheaper than buying commercial ink?
HYPOTHESIS H0: The amount spent on Mayana (Coleus blumei) in not cheaper
compared to commercial ink
H1: The amount spent on Mayana (Coleus blumei) is cheaper
compared to commercial ink.

H0: There is no significant difference between Mayana (Coleus

blumei) extract and commercial ink as an alternative ink for
markers.
H1: There is a significant difference between Mayana (Coleus
blumei) extract and commercial ink as an alternative ink for
markers.
SIGNIFICANCE OF The findings of this research will yield alternative inks, which will
benefit all people and provide affordable, non-toxic alternatives to
synthetic inks using easily accessible materials.
THE STUDY
The results of the study will be used for:
Consumers. The eco-friendly Mayana leaf ink is particularly
beneficial for consumers it offer vibrant colors without skin
irritation.
Painters. Can benefit from its natural pigments, diverse palette,
and cost-effectiveness.
Students. It ensures safety, affordability, cultural connection, and
aesthetic appeal, promoting holistic learning experiences.
Teachers. Find value in Mayana ink’s natural, non-toxic, and
sustainable benefits, fostering traditional methods and innovative
learning environments.
 This study seeks to determine the
DELIMITATION
SCOPE AND
efficacy of extract from Mayana
(Coleus blumei) leaves as an
alternative pigment for Lip tint,
Makers, and Ballpen ink ,
examining its efficacy compared
to commercial inks.
 01 Studies
Alguzar et al. (2015) found that
LITERATURE
Mayana leaves offer a non-toxic,
cost-effective alternative pigment
REVIEW
ink with vibrant colors, reducing
environmental impact. Plant
extracts, including Mayana, prove
promising for sustainable marking
ink.
1.Antigua et al. (2021) suggest
Malunggay and Mayana leaves
as eco-friendly alternatives to
chemical inks in markers,
showing comparable results
with commercial ink but with
potential for sustainability.
02 Literature
Shaw, P. (2000)43 investigates the
possibility of using plant material to make
pigments that could replace CMYK colors
in screen printing. It examines how pre-
synthetic dye plants are used in the textile
and associated sectors, pointing out a
number of plant sources that need
further research.
1. Christiansen, R (2017) explores ancient
Mediterranean black pigment
manufacturing using “carbon ink,”
“mixed ink,” and “iron gall ink” through
historical texts, including Vitruvius,
Pliny, and Dioscorides. The compounds
mentioned had applications in
pharmacology. Soot and charcoal for
“mixed inks” were byproducts of
metallurgy, glaze, and glass production.
The article includes a glossary of
technical terms.
 INPUT PROCESS

• Mayana Leaves
CONCEPTUAL
FRAMEWORK
• Water 1. Pick out a Mayana leaves to extract.
• Pot or Saucepan 2. To prepare a large plant source, use a
• Spoon knife and cutting board to finely chop it
• Dropper into small pieces.
• Empty Refillable Marker 3. Add enough water to cover the plant
• Bottle or any container source and ensure tea or spice is
• 1 tsp of Vinegar combined with liquid, not absorbing or
• Strainer turning into a paste.
• Stove 4. Heat mixture on stove for 15-20 minutes,
allowing water to turn color due to plant
material's pigment.
5. Heat water without the pan's lid until
enough liquid evaporates, leaving behind
OUTPUT a darker liquid.
6. Remove the saucepan from heat, let the
dye cool, and if the source disintegrates,
• Liptint pour the resulting liquid into a basin.
• Markers 7. You're ready to use your own ink in the
• Ballpen marker now that you have it.
GAP BRIDGE This study explores internet-published
research on plant extracts as pigments
for various products. While similar
studies exist, none specifically
investigate Mayana (Coleus Blumei)
leaves for lip tint and markers. The
focus is on Mayana ink, assessing color
intensity, stability, durability, and
longevity to address a research gap.
RESEARCH DESIGN
The researchers used experimental research design. Shrutika Siri Silla
(2023) states that a scientific approach to conducting experimental
research with two sets of variables is achieved through the use of
experimental research design. Moreover, according to (Mitchell, 2015)
the main goal of experimental research design is to create studies with a
high degree of causal (internal) validity. Experimental research aids in
understanding cause-and-effect relationships by manipulating variables
(Bhat, 2023). Longe (2020) stated that diverse data collection methods in
experimental research. The objective of experimental research,
according to Valentine-French (n.d.) is providing definitive results on
causal relationships. This experimental research aimes to investigate the
potential of Mayana leaves (Coleus blumei) as an organic pigment for
various applications, specifically focusing on its suitability for lip tint,
markers, and ballpen ink. It follows a quantitative approach, using
experiments to assess Mayana's efficacy against commercial products.
Data collection, experiments, observations, and group analysis support
conclusions on the extract's effectiveness. 10
METHODS AND
VALIDATIONS
The research aims to validate Mayana (Coleus blumei
Benth) leaf extract as an organic pigment for lip tint,
markers, and ballpen ink, addressing the high cost of
these products. This study responds to the demand for
affordable alternatives among students, teachers,
designers, painters, printers, artists, and the general
public. The researchers, approved by their Practical
Research 2 teacher, conducted experiments to test the
effectiveness of Mayana leaf extract in producing
organic pigments for lip tint, markers, and ballpen ink.
 A. Collection of Mayana Leaves
GATHERING B. Preparation of Materials
DATA

C. Experimental Set - Ups

D. Extraction Method Use

E. Making the Liptint and Whiteboard Marker

F. Testing of Products
 ANOVA is a statistical tool that divides observed variability in a data set into systematic and
random factors. Systematic factors have statistical influence on the data set, while random
factors do not. Analysts use ANOVA to determine the influence of independent variables on
STATISTICAL
TREATMENT the dependent variable in a regression study (Kelton, W. 2023)6. In this case the researcher
will use the ANOVA formula as follows:

Let’s consider three groups:

Group 1: Mayana leaves pigment as a lip tint
Group 2: Mayana leaves pigment as ballpen ink
Group 3: Mayana leaves pigment as whiteboard marker ink

The null hypothesis (H0) is that there is no significant difference among the means of the
three groups, while the alternative hypothesis (H1) is that at least one group mean is
different from the others.

The ANOVA formula for a one-way ANOVA can be expressed as:

Where:
•F is the test statistic for ANOVA,
•MSB is the mean square between groups (variance between groups divide by the degrees
of freedom between groups),
•MSW is the mean square within groups (variance within groups divided by the degrees of
freedom within groups).
THANK
YOU