Assi

gnmentofMED-
403
Name:AQSAABDEEN
RegNo:2k16-
AV-
18
Topi
c:Aspar
tat
eAmi
not
ransf
erase
(
AST)Test
Submi
tt
edTo:Si
rNazarAl
iKor
ejo

Aspar
tat
eAmi
not
ransf
erase(
AST)
Whati
saspar
tat
eami
not
ransf
erase?
Ami notransfer
ase(AST)isanenzymet hat’
spresent
i
nv arioustissuesofAnimal’
sbody.Anenzy meisa
protei
nt hathelpstr
iggerchemical
reacti
onsthat
animal ’
sbodyneedst ofuncti
on.
ASTisfoundinthehighestconcentrat
ionsinthe
l
iver,
muscles,
heart,
kidney,brai
nandr edbloodcell
s.
Asmal l
amountofASTi stypical
lyinthe
bl
oodstream.Higher
-t
han-normal amountsofthis
enzymeinthebloodmaybeasi gnofaheal th
probl
em.Abnormal l
evel
scanbeassoci at
edwi t
h
l
i
veri
njur
y.
ASTl evel
sincreasewhenthere’sdamaget othe
ti
ssuesandcel lswheret
heenzy mei sf ound.AST
l
ev el
scanr i
seassoonas  6hour s af
terdamaget o
ti
ssueoccur s.Thenormalrangef orASTi shigher
from bir
thtoage3compar edt ot henormal r
anges
forolderandadults.
TheASTtestmeasur est heamountofASTi nt
he
bl
oodthathasbeenr eleasedfrom i
njur
edti
ssue.An
ol
dernamef orthetestisserum gl
utamic-
oxal
oacet
ictransaminase( SGOT).

ASTBl
oodTest
Thebl oodt estf orASToraspar tat
et r
ansami naseisat est
tor evealinj uryt o certainor gans,speci fi
callythel iver.
Aspartatetr ansami naseisanenzy methati sr esponsi ble
forthecr eat ionofgl utamat eandoxal oacet at
e.Bot hof
thesear einput st hatgoi ntoacr uci
alsy stem cal l
edt he
citr
icacidcy cle.Thi sisthecy clewhereener gyiscr eat ed
byt hecr eat ionofguani net r
iphosphateandadenosi ne
tri
phosphat e. These ar e t hen conv erted i nto t heir
diphosphatef ormsandener gyisr el
eased.ASTi spr esent
i
nnear lyallcel ls,butisav ail
ableintheliver,hear t
,andt he
brain abundant ly
.Whi let hi
st estwas i nit
iall
yused t o
checkforhear
tdiseasei
tisnow usedt
ocheckf
oronl
y
l
iveri
nfl
ammation.
ReasonWhyI
tisPer
for
med
TheAST t esti sused when a pat ientturnsup att he
doctor
’sof f
icewi t
hacaseofj aundice.Oneoft hef ir
st
testst
hatar eor deredi sagammagl utamyltr
ansami nase
testal
ongwi thanal aninet
ransaminasetestoranal kali
ne
phosphat easet est.Al loft
hesetestswhencombi nedwi ll
giveacl eari ndicati
onofl iverdamaget hatiscausedby
acuteinflammat ion.Thi si
susual l
ywhathappenswhen
therei
sal i
veri nfectionasisthecasei nvi
ralhepati
tisbut
i
tcanal sobebecauseofal
ASTisanenzy methathel
psmet abol
izeaminoacids.
Li
ke ALT,ASTisnormall
ypresenti
nbl oodatlowl
evel
s.
Anincreasei
nASTl ev
elsmayi ndi
cat
e  l
i
verdamage,
di
seaseormuscledamage.
Al
soKnownas:
 
AST, 
Ser
um Glutami
c-Oxal
oacet
icTr
ansami
nase,
 
SGOT,
 
GOT,
 
Aspar
tat
e
Tr
ansaminase,
 
ASAT
Pr inci pl e:
Aspar tat eami notransferase(AST)cat al
y zesthe
transf eroft heami nogroupfrom L-aspar tat
eto-
ketogl ut aratetoy i
eldoxaloacetateandL- glut
amate.
Mal atedehy drogenase( MDH)cat al
yzest he
reduct ionofoxal oacetatewithsimultaneous
oxidat ionofNADH+t oNAD.Ther esulti
ngr at
eof
decr easei nabsor banceat340nm i sdirectl
y
propor tional t
ot heASTact i
vit
y.Lactate
dehy dr ogenase( LDH)i saddedt oprevent
i
nt erfer encef rom endogenouspy r
uvatewhi chis
nor mal lypr esenti nserum.
KitCont ents:
 R1:SGOTEnzy meReagent
 BufferTris,pH=7. 5—–125mmol /
L
 L-aspartate————600mmol /
L
 LDH————–≥0. 9U/ L
 MD H——————≥0. 6U/ L
 Detergent,preservat
ive.
 R2: SGOTSubst r
ateReagent
 NADH—–0. 18mmol /L
 α-ketoglutarate—-15mmol /L
 Detergent,preservat
ive.
TestRequi
rement
s:
 TestTubes
 Micropi
pettes
 Ti
ps
 Bi
o-Chemi str
yanaly
zer
 SGOTTestKi t
 Cali
brat
or
ReagentPr eparati
onandSt
abi
l
ity:
Wor ki
ngReagent :
4Par tofR1( 0.8ml)
1Par tofR2( 0.2ml)
andMi xwel l
Avoiddirectexposuretol
ight
.Stabi
li
tyofwor
king
reagent:3day sat2–8° C.
Specimencol lect
ionandhandl
ing:
1.
Non-hemolyzed 
serum,hepar
ini
zed
or 
EDTA plasma i
srecommended.
2.
Stabil
i
ty:
10day sat2–8°C.
Analy
zerParameterRequir
ed:
React
ionTy
pe Ki
net
ic(
decr
easi
ng)
Wav
elengt
h 340nm (
334nm –365nm)

Cuv
ett
eTemp 37°
C

Del
ayTi
me 60sec

I
nter
val
Time 60sec
No.Ofr
eadi
ng 2

Zer
oSet
ti
ng Agai
nstai
rorDei
oni
sedWat
er

Li
ghtPat
h 1cm

Fact
or 1746

TestPr
ocedur
e:
Pi
pett
eintocl
eandr
ytestt
ubel
abel
edas(
T)andCal
i
brat
orLabel
(c)andQCLabel
(QC)or
asyourr
equir
ed:

Speci
min (
T) (
C)/(
QC)

1.
0ml 1.
oml
Wor
kingReagent
Pt
.Sampl
e 0.
1ml –

Cal
i
brat
ororQC – 0.
1ml

Mixwel
landreadt
heini
ti
alabsor
banceaft
er1minandr
epeattheabsor
bancer
eadi
ngaf
terev
ery1,
&2m
Cal
cul
atethemeanabsor
bancechangeperminut
e(ΔOD/min.
).

CALCULATI ON: SGOTact i
vit
y(U/L)=DA/ mi
n.x
1746
NOTE: Sampleshavingav er
yhighacti
vit
yshowa
veryl
owi ni
ti
alabsorbanceasmostoft heNADHis
consumedpr i
ortothestartofthemeasurement
.If
thi
sissuspectedthendilut
ethesampleandrepeat
theassay
LI
NEARI
TY:
Theprocedureisl
inearupto300U/L.
i
ftheacti
vit
yexceedst hi
sli
mitdi
lut
ethesample
wit
hnormal sal
ine(NaCl0.9%)andmulti
plyr
esul
t
bydil
uti
onfactor.
QUALI
TYCONTROL:
Foraccuracyi
tisnecessar
ytor
unknowncont
rol
s
wit
hev er
yassay.
Nor
mal
Val
ue:
Ser
um : <40U/L
Note:
 EachLabor
ator
yorEachCompanyKi
tsshoul
d
est
abli
shit’
sownnormalrange.

Nor
malr
angesofASTi
ndi
ff
erentani
mal
s….

AST 
i
susef
ulasani
ndi
cat
or 
of 
l
iverand/
ormuscl
e

i
njur
y i
n l
argeandsmal
l
 ani
mal
s.

Theenzy
mehal
fli
fei
sabout

22hour
s i
n t
hedog,

77mi
nut
es 
i
n t
hecat
,
7-
8day
s i
n hor
sesandar
ound

1day
 i
n cat
tl
e…

REFRENCEANDLI
NKS;

ht
tps:
//y
out
u.be/
DrLmGHPVgwc
ht
tps:
//y
out
u.be/
v5ALGK1Z6Cw