S-65.02.0018A (4.0) DH76 Auto Hematology Analyzer Service Manual

Auto Hematology Analyzer
Service Manual
Preface
Thank you for purchasing the Auto Hematology Analyzer manufactured by Dymind Biotech.
Before performing maintenance on the device, please read this service manual carefully to gain an
understanding of how to properly maintain it.
After reading, keep the manual in a convenient location for future reference and easy access.
Product name: Auto Hematology Analyzer

Model: DH71, DH76
Contact Info for After-Sales Services

Shenzhen Dymind Biotechnology Co., Ltd.
2/F, Nanfeng Building B, Nanshan Yungu Innovation Industrial Park, No.1183, Liuxian
Blvd, Taoyuan Street, Nanshan District, Shenzhen 518055, P.R.China
Landlink GmbH
Dorfstrasse 2/4, Emmendingen
Tel: (86-755)26989825
Service Tel: 400-998-7276
Fax: (86-755)26746162
Email: dymind@dymind.com.cn
Website: http://www.dymind.com.cn/english/
Intellectual Property Rights

This document contains proprietary information of Shenzhen Dymind Biotechnology Co., Ltd.
(hereinafter referred to as Dymind Biotech).The corresponding product is the intellectual property of
Dymind Biotech.
No part of this service manual may be reproduced, copied, modified or translated in any form or by
any means without prior written consent of Dymind Biotech.
D&M and Dymind are trademarks of Dymind Biotech.
i
Declaration
Dymind Biotech reserves the right of final interpretation of this service manual.
Dymind Biotech shall be responsible for the safety, security, and performance of the product only
when all of the following conditions are met:
 The assembly, re-commissioning, extension, modification, and repair of the product are
performed by authorized personnel of Dymind Biotech.
 The installation of the relevant electrical devices complies with applicable national standards.
 The product is operated in accordance with this service manual.
Maintenance Services
Scope of free services:
All products complying with Dymind Biotech’s Product Warranty specifications may qualify for free
services.
Scope of fee-based services:

 Free services are available to all products exceeding Dymind Biotech’s Product Warranty
specifications.
 Products within the warranty period require maintenance under the following circumstances:
 Artificial damage
 Improper use
 When grid voltage exceeds the specified operational range
 Unavoidable natural disasters
 When parts and supplies are replaced with no prior consent from Dymind Biotech, or
machine maintenance is performed by personnel with no prior authorization from Dymind
Biotech
 Any failure by hospitals or organizations responsible for using this instrument to implement a
competent repair/maintenance plan is likely to result in abnormal instrument failure or even a
health hazard.
 Make sure the analyzer is only operated under the conditions of use as specified in the
operator's manual. If operated outside of the specified conditions of use, the analyzer may not
work properly, which could lead to unreliable measurement results and damaged instrument
components or even bodily injury.
This analyzer must be operated by trained medical testing professionals, doctors, nurses, or
laboratory technicians.
ii
Contents
Contents
Contents ............................................................................................................................................ iii

1 Manual Overview ............................................................................................................................ 1
1.1 Who Should Read This Manual ............................................................................................... 1
1.2 Symbols and Legends.............................................................................................................. 1
1.3 Safety Instructions.................................................................................................................... 4
2 Instrument Configuration .............................................................................................................. 6
2.1 Mechanical Components.......................................................................................................... 6
2.2 Hydraulics System ................................................................................................................... 9
2.3 Hardware System..................................................................................................................... 9
2.4 Software System .................................................................................................................... 10
3 Hydraulics System ........................................................................................................................ 11
3.1 Introduction ............................................................................................................................. 11
3.2 Principle of the Hydraulic System ........................................................................................... 11
3.3 Hydraulics Diagram for the Overall Unit................................................................................. 12
3.4 Time Sequence Description ................................................................................................... 12
3.4.1 DIFF Measurement Channel.......................................................................................... 12
3.4.2 WBC/HGB Measurement Channel ................................................................................ 13
3.4.3 RBC/PLT Measurement Module .................................................................................... 14
3.4.4 Sampling and Blood-dispensing Module ....................................................................... 15
3.4.5 Power Supply and Waste Discharge Module ................................................................ 15
3.4.6 Status Monitoring Module .............................................................................................. 16
3.4.7 Hydraulic Components ................................................................................................... 16
3.4.8 Main Measurement Modes and Procedures .................................................................. 24
3.5 Hydraulics Maintenance ......................................................................................................... 34
3.5.1 Swab Cleaning and Maintenance .................................................................................. 34
3.5.2 Cleaning and Maintenance of the WBC Bath ................................................................ 34
3.5.3 Cleaning and Maintenance of the RBC Bath ................................................................. 35
3.5.4 Cleaning and Maintenance of the DIFF Bath................................................................. 35
3.6 Troubleshooting Common Hydraulics Problems .................................................................... 36
3.6.1 Commonly Used Equipment and Tools .......................................................................... 36
3.6.2 Inspection and Troubleshooting of Valve Clogging ........................................................ 36
3.6.3 Liquid Pump Clogging: Inspection and Troubleshooting ............................................... 37
3.6.4 Aperture Clogging of the WBC Channel ........................................................................ 38
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Contents
3.6.5 Aperture Clogging of the RBC Channel ......................................................................... 39

3.6.6 Clogging of the Flow Chamber/DIFF Probe................................................................... 40
3.6.7 Overflowing of the WBC Bath ........................................................................................ 42
3.6.8 Overflowing of the RBC Bath ......................................................................................... 43
3.6.9 Overflowing of the DIFF bath ......................................................................................... 44
3.6.10 Leakage from the Swab ............................................................................................... 44
3.6.11 Problems with Creating Positive Pressure ................................................................... 46
3.6.12 Problems in Creating the Negative Pressure............................................................... 46
3.6.13 Clogging of the Air Filter............................................................................................... 47
3.6.14 No Scattergram ............................................................................................................ 48
4 Hardware System ......................................................................................................................... 49
4.1 Hardware System................................................................................................................... 49
4.2 Main Control Panel................................................................................................................. 50
4.2.1 Composition of the Main Control Panel ......................................................................... 50
4.2.2 Peripheral Interface of the Main Control Panel .............................................................. 51
4.2.3 Power Input and Indicator Lights on the Main Control Panel ........................................ 52
4.2.4 Testing Points on the Main Control Panel ...................................................................... 53
4.2.5 Identification of Main Control Panel Problems ............................................................... 56
4.2.6 Maintenance of the Main Control Panel......................................................................... 64
4.3 Driver Panel ........................................................................................................................... 68
4.3.1 Overview of the Driver Panel ......................................................................................... 68
4.3.2 The Indicator Lights for the Driver Panel ....................................................................... 70
4.3.3 Main Testing Points of the Driver Panel ......................................................................... 72
4.3.4 Identification of Driver Panel Problems .......................................................................... 73
4.3.5 Maintenance of the Driver Panel ................................................................................... 78
4.4 Rear Panel ............................................................................................................................. 80
4.5 Autoloader .............................................................................................................................. 81
4.6 Reagent Testing Panel ........................................................................................................... 82
4.7 Indicator Panel ....................................................................................................................... 83
4.8 Key Panel ............................................................................................................................... 84
5 Optical System ............................................................................................................................. 85
5.1 Integral Replacement of the Optical Assembly ...................................................................... 85
5.2 Replacing the Optical Preamplifier Panel .............................................................................. 87
5.3 Replacing the Laser Driver Panel .......................................................................................... 88
5.4 Fine Tuning of the Flow Chamber .......................................................................................... 90
5.5 Common Problems and Solutions ......................................................................................... 92
6 Repairs .......................................................................................................................................... 94
6.1 Introduction ............................................................................................................................ 94
6.2 Preparatory Work Before Repairs .......................................................................................... 94
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Contents
6.2.1 Open the Left Side Door ................................................................................................ 94

6.2.2 Open the Right Side Door .............................................................................................. 94
6.2.3 Opening the Top Panel Cover ........................................................................................ 95
6.2.4 Disassembling the Base Panel Cover ........................................................................... 96
6.3 Replacing the Sampling Assembly ......................................................................................... 97
6.3.1 Replacing the Sample Probe ......................................................................................... 97
6.3.2 Replacing the Optocoupler ............................................................................................ 99
6.3.3 Replacing the Sampling Assembly in X- or Y-direction .................................................. 99
6.4 Replacing the Power Assembly ........................................................................................... 101
6.5 Replacing the Hydraulics Components incl. Valves, Pumps, and Pressure Chambers ...... 103
6.5.1 Replacing the Valve Assembly ..................................................................................... 103
6.5.2 Replacing the Air Pump Assembly ............................................................................... 104
6.5.3 Replacing the Liquid Pump Assembly ......................................................................... 105
6.5.4 Replacing the Positive-pressure Chamber Assembly .................................................. 107
6.5.5 Replacing the Negative-pressure Chamber Assembly ................................................ 109
6.6 Replacing the Sheath Flow Syringe Assembly ..................................................................... 110
6.6.1 Replacing the Syringe ................................................................................................... 110
6.6.2 Replacing the Motor ...................................................................................................... 111
6.7 Replacing WBC and RBC Bath Assemblies ......................................................................... 113
6.7.1 Dismantling and Replacing the WBC Bath Assembly ................................................... 113
6.7.2 Dismantling and Replacing the RBC Bath Assembly ................................................... 116
6.8 Dismantling and Replacing the Mix Assembly ...................................................................... 118
6.9 Replacement of DIFF Bath Assembly .................................................................................. 122
6.10 Dismantling and Replacing the Sample Transfer Unit ........................................................ 123
6.11 Replacement of the Main Control Panel ............................................................................ 130
6.12 Replacement of the Driver Panel ....................................................................................... 131
6.13 Replacement of Reagent Testing Panel............................................................................. 132
6.14 Replacing the Sheath-flow Sensor and Temperature Sensor ............................................ 134
6.14.1 Disassembling and Replacing the Sheath-flow Sensor ............................................. 134
6.14.2 Disassembling and Replacing the Temperature Sensor ............................................ 135
7 Software Installation and Upgrade ........................................................................................... 137
7.1 Software Installation ............................................................................................................. 137
7.1.1 Preparation................................................................................................................... 137
7.1.2 Procedure for Software Installation .............................................................................. 137
7.1.3 Troubleshooting Software Installation .......................................................................... 139
7.2 Software Upgrade ................................................................................................................ 139
7.2.1 Upgrading the Main System......................................................................................... 139
7.2.2 Upgrading the Software Interface ................................................................................ 141
7.2.3 Troubleshooting Host Upgrade .................................................................................... 143
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Contents
8 Comprehensive Device Debug ................................................................................................. 145

8.1 Position Adjustment.............................................................................................................. 145
8.2 Mix Position Adjustment ....................................................................................................... 145
8.3 Barcode Scanner Debugging ............................................................................................... 147
8.4 HGB Voltage Gain Setting.................................................................................................... 147
8.5 Gain Calibration ................................................................................................................... 148
8.6 Calibration of Calibrators...................................................................................................... 149
8.6.1 Calibration in Whole-blood Mode ................................................................................. 149
8.6.2 Calibration in Predilute Mode ....................................................................................... 150
8.7 LIS Connection..................................................................................................................... 150
8.7.1 Preparation................................................................................................................... 150
8.7.2 Installing LIS Workstation............................................................................................. 150
8.7.3 Connecting the Analyzer Main Unit and Software ....................................................... 150
8.7.4 Connecting Analyzer Software with LIS ....................................................................... 154
9 Alarms and Solutions ................................................................................................................ 156
9.1 Trouble Analysis and Solution .............................................................................................. 156
9.2 Trouble Analysis and Solution on Network Connections ..................................................... 166
Appendix A Maintenance Inventory ............................................................................................ 170
A.1 PCBA List ............................................................................................................................. 170
A.2 List of Cables and Wires ...................................................................................................... 170
A.3 List of Wearing Parts ........................................................................................................... 172
A.4 List of Consumable Parts..................................................................................................... 173
A.5 List of Parts and Assemblies................................................................................................ 175
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1 Manual Overview
1 Manual Overview
This chapter explains the procedures for maintaining Auto Hematology Analyzer. Please read this
manual carefully to guarantee proper device maintenance and operator safety.
This manual is complemented by the operator's manual; there is no overlap between the two.
Be sure to operate the device in strict accordance with the instructions in the maintenance and
operator's manuals.
1.1 Who Should Read This Manual

This service manual should be used by professionals who possess:
 A comprehensive knowledge of electrical circuits and hydraulics
 A comprehensive knowledge of reagents
 A comprehensive knowledge of quality control
 A comprehensive knowledge of troubleshooting
 The ability to skillfully operate this analyzer
 An understanding of basic mechanical tools and any relevant terms
 Skills for using digital multimeter and oscilloscopes
 The ability to analyze electrical-circuit graphs and hydraulics diagrams, and to understand the
related terminology
1.2 Symbols and Legends

Symbols used in the manual:
Symbol Meaning
The operator should follow the instruction below each symbol to

avoid personal injury.
The operator should follow the instructions below each symbol to

prevent instrument failure, damage, or disrupted test results.
1
1 Manual Overview
Symbol Meaning
The operator should follow the instructions below each symbol and
pay special attention to any pertinent information when following the
procedures.
The operator should follow the instructions below each symbol to

prevent any danger of infection.
Laser Warning:
This sign serves as a reminder of laser radiation.
The following symbols can be found on the analyzer, reagents, QC materials, or calibrator:
 If the labels are damaged or missing, please contact Dymind or Dymind’s agents for
replacement.
 All illustrations in this manual are provided as references only. They may not necessarily reflect
actual analyzer configuration or display.
When you see It means
Caution
Biohazard
Exercise caution to prevent puncture
Laser radiation warning:

It is a Class 3R laser product with 5.0 mW of maximum power output
at 635nm. Do not stare into the laser beam or view directly with
optical instruments.
Instruction for Moving
Network interface
Protective grounding
Alternating current (AC)
For in vitro diagnosis only
2
1 Manual Overview
Lot No.
Exp. date
Serial No.
The device is in full compliance with the council directive concerning

in vitro diagnostic medical devices 98/79/EC.
Authorized Representative in the European Community
Date of manufacture
Manufacturer
Storage temperature
Humidity level for storage
Atmospheric pressure level for storage
Consult the operator’s manual
Avoid sunlight
Keep dry
No rolling
No Stacking.
Let this side face upward.
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1 Manual Overview
Fragile, handle with care
Recyclable materials
The analyzer, after being scrapped, should not be disposed with

other household garbage, instead, it should be collected and
recycled following the disposal instructions for scrapped electronic
and electrical equipment.
1.3 Safety Instructions

Device maintenance done in accordance with the following instructions guarantees the safety of both
patients and operators.
 Note: Any failure by hospitals or organizations responsible for using this instrument to implement
a competent repair/maintenance plan is likely to result in abnormal instrument failure or even a
health hazard.
 To prevent risk of explosion, do not use combustible gases (e.g. anesthetics) or liquids (e.g.
ethanol) near this product.
 The machine should be powered off while troubleshooting. Any maintenance operation while the
power is on can lead to electric shock or damage to its electrical components.
 Please connect the device to the socket using a separate fuse and surge protection switch. If the
device shares a fuse and surge protection switch with other equipment, e.g. life support
equipment, any malfunction may cause an electric surge to occur when the instrument is
powered on, which can trip the circuit breaker.
 Maintenance personnel must keep their clothes, hair, and hands away from moving parts such
as the sample probe, jaws, and puncture needle to avoid being stabbed or pinched during
maintenance.
 Parts with special warnings may be subject to mechanical movements and thus lead to injuries
from pinching or stabbing during normal operation or disassembly and repair.
 Operators are obligated to comply with local and national regulations with regard to the disposal
and emission of expired reagents, waste, waste samples, consumables, and so on.
 Reagents can be irritating to the eyes, skin, and mucosa. When handling reagents and their
related items in the laboratory, the operator should comply with laboratory safety regulations and
wear personal protective equipment (such as a protective lab suit, gloves, etc.).
 If the reagent accidentally comes in contact with your skin, wash it off immediately with plenty of
water and see a doctor if necessary. Do the same if you accidentally get any of the reagent in
your eyes.
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1 Manual Overview
 Improper maintenance may damage the analyzer. Maintenance personnel should maintain the
device in accordance with the instructions contained in the service manual, and inspect the
device properly after each maintenance.
 If you encounter a problem not specified in the service manual, please contact Dymind’s
aftersales service department. A professional will be assigned to offer you maintenance advice.
 When repairing the electronic components of the device, please take off any metal accessories
you might be wearing to prevent personal injury or damage to the device.
 Electrostatic discharge may cause damage to the device’s electronic components. If the repair
process can result in any electrostatic damage, please wear an antistatic wrist strap or maintain
the device on an antistatic workstation.
This analyzer must be operated by trained medical testing professionals, doctors, nurses, or
laboratory technicians.
 Samples, controls, calibrators, and waste may pose a potential risk of bio-contamination. When
handling reagents and related items in the laboratory, the operator should comply with laboratory
safety regulations and wear personal protective equipment (such as a protective lab suit, gloves,
etc.).
 Both the parts and surface of the analyzer are potentially infectious, so please take safety
precautions during operation and maintenance.
 The pointed sample probe, any blood stains left on it, QC materials, and calibrators may be
potentially bio-infectious, so please avoid contact with the sample probe.
This sign warns of laser radiation.Do not look directly at the laser beams or see through the optical
system.
5
2 Instrument Configuration
2.1 Mechanical Components

The 5-part differential hematology analyzer consists of the main unit and its accessories.
Figure 2-1 Front view of the analyzer
1 - Aspirate key for open-vial sampling 2 - [RUN] key for autoloader sampling
3
3 - Status indicator light
6
Figure 2-2 Back view of the analyzer
1 - Diluent inlet 2 - LYA-1 lyse inlet
3 - LYA-2 lyse inlet 4 - LYA-3 lyse inlet
5 - BNC socket for the diluent sensor 6 - Power input socket
7 - Waste outlet 8 - BNC socket for the waste sensor
9 - LAN network interface
7
Figure 2-3 Front view of the analyzer (housing removed)
2 4
1 - Sheath flow syringe assembly 2 - Small valve assembly
3 - Sample probe assembly 4 - Mix assembly
5- Sample Transfer Unit 3
2 4
Figure 2-4 Right-side view of the analyzer (right-side door removed)
1 - DIFF reaction bath assembly 2 - Impedance-counting reaction bath

assembly
8
3 - Filter 4 - Negative-pressure pump assembly
5 - Negative-pressure chamber 6 - Large valve assembly
7 - Positive-pressure chamber 8 - Positive-pressure pump
9 - Optical assembly 10 - Autoloader Panel Unit
11 - Mix assembly 12 - Sample probe assembly
Figure 2-5 Left-side view of the analyzer (Left-side door removed)
1 - μl syringe assembly 2 - Diluent syringe assembly
3 - Lyse syringe assembly 4 - Liquid-level detecting assembly
5 - Power switch assembly 6 - Driver panel assembly
7 - Main control panel assembly
2.2 Hydraulics System

Refer to "3 Hydraulics System".
2.3 Hardware System

Refer to "4 Hardware System".
9
2.4 Software System

Main Menu Description
Access Codes Description
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3 Hydraulics System
3 Hydraulics System
3.1 Introduction
The hydraulics system consists of the DIFF measurement module, the WBC/HGB measurement
module, the RBC/PLT measurement module, the sampling and blood dispensing module, the power
supply and waste discharge module, and the status monitoring module. Detailed description:
 DIFF measurement module:
Consists of the sheath flow syringe, sample syringe, flow chamber, DIFF reaction bath, tubing,
and valve.
 WBC/HGB measurement module:
Consists of the diluent syringe, WBC/HGB counting bath module, tubing, valve, HGB emission
light, and HGB receiving tube.
 RBC/PLT measurement module:
Consists of the diluent syringe, RBC/PLT counting bath assembly, tubing, and valve.
 Sampling and blood dispensing module:
Consists of the sample probe, sample syringe, swabs, and sampling assembly.
 Power supply and waste discharge module:
Consists of the vacuum chamber, liquid pump, valve, and tubing.
 Status monitoring module:
Consists of the optocoupler and sensor.
3.2 Principle of the Hydraulic System

Whole-blood
CBC+DIFF Mode
Sampling
Diluted sample
Flow chamber
Dispensing Dispensing
DIFF WBC/HGB RBC/PLT

measurement measurement measurement
Sample flow syringe
Sampling syringe
module module module

Sheath flow syringe
LYA-3 LYA-2 LYA-1

Negative-pressure
chamber
Diluent
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3 Hydraulics System
3.3 Hydraulics Diagram for the Overall Unit

3-way valve 2-way valve
3 1 2:COM 1
Hydraulics Diagram J12 T70 J11 J7 T79
2:IN T76
1:NC 1:OUT J12 T70 J11 J6 T79
2 T76
2 3:NO
J12 T78 J12 T70 J11 J8 T79
J3 T53 J10 J9 T14
J2 T29 T42-2
T70 T43
T1 T25-1 J3 T3 J3 J34 J35 J36
sheath flow T44-1 J33
pressure J12 T2 T80 T2 T2 T72 T72 T72
Flow
sensor T45 T45 T21-3
T2 LV5 LV6 T15
chamber
T33 T80 T80 T80
T23-1
T40 T70 LV7 LV3 T12 T13 T13
T70 T70 T70 T70
T70 J1 LV2 LV1
T41 T29 T30 T80
T21-1 LV8 Diluent
LV11 T70 J3 T26 LV4 J12 T75 T75
LYA-3 LYA-2 LYA-1
T80 T4
T25 T2
LV12 T23 J1 T74
LV10 J1 J1 J1
T22-3 LV9 J12
T24 T29
T22-1 T47 T44 Air pump Air Filter
T22-2 J3 Diluent
J3 T31-1 T80 Lyse T67
T32 syringe T88 J5 T80 J13 T57 T70 T70
T21-2 LV13 syringe
T29
J1 J1 J1 (10ml) (2.5ml) One-way J5
T67
valve
Sheath
T73 PP03
T29 positive-pressure T39
Flow Syringe
Sampling J1 J1 sensor casing pipe
(10ml) Sample flow T8 T82
syringe T52
(250ul) syringe Air Filter
T52 T22
(100ul)
casing pipe strapping T80 T80
T9 T67 T67
T70 T42-1
T20 LV27 T80 T80 J5 T19 J78
J48
T73 LV28 J12
T81 T73 T73 T49 T67 LV17 T29
casing J46 J47 T50
pipe T29 T49 T82 T35 positive-pressure
T9 T35 T35 T85 T70 chamber
LV18 RBC T80 LV20 T62 T66 T29
T29 T70 T29 T82 T80 T80 T80
T29
WBC
T80 T80
J5 LV24 J12 PP01 liquid pump
T51 waste
DIFF T29 T29 J5 T80 J5 J13 T16 T14
T47 T80 T82 T83 T67 T18
T54 T29 T29 T38 T57 T63
swab J3 J3 T83 J51 T70 T66
T70 T82 T10 T48 J3 J60
T29 J5 T70 J10
T69 T10 T48 T80 liquid pump
J13 J1 T29 T70 PP02
T70 T29 T80
T84 T50 T70 T90 J14
T29 T70 T29 T29 Isolation T84 T50 T70 J13 T18 J4
T9 In-line T59 T11 LV16 T87 T17
sample T35 T48 T37 J3 chamber T59 negative-
T35 J12 J61
probe T9 filter T80 T29
T28 In-line J52 T11 In-line LV23 pressure T62
Isolation
T27 T11 LV15 filter T9 T11
chamber
J13 chamber T57 LV22 filter T9 J13 T57 T57 J5 Note：
J12 J13 T70
T55 T9 J13 T65 T2 T9 T2 T50 LV-Liquid valve； PP-pump；
In-line filter T9 J13 T29
J5 J13 T65
T31-2 LV21 T66 negative-pressure
T57
T-pipe； J-connector
T66 LV26 sensor
LV19 T9 J13 T29 T62
T58
LV14 LV25 T62 T86 T63 LV29 ECN No. Rev Design Check Approval Date
T56 T66
T7 J5 J3
LV30 T3 J3 T22
T63 J5 T62 J5
T67
T64
3.4 Time Sequence Description
3.4.1 DIFF Measurement Channel
3.4.1.1 Channel Principles and a Description of Their Functions

 Measurement principles: Flow cytometry and scattering emission of the semiconductor laser
 Measurement parameters: Mon#, Mon%, Lym#, Lym%, Neu#, Neu%, Eos#, Eos%, Bas#, Bas%
 Graph information: one WBC 3D scattergram, three WBC 2D scattergrams
 Reagents for use
 LYA-3 & LYA-2 lyse: Lyses the RBCs and specifically treats different types of WBCs.
 Diluent: Provides sheath flow fluid, used for cleaning.
 Function description
The sample is mixed well for a full reaction with LYA-3 lyse and LYA-2 lyse in the DIFF bath.
Then the sample is placed between the flow chamber inlet and the sample injection tube;
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3 Hydraulics System
meanwhile, the sheath flow is activated and kept steady so that it can hold the sample in motion
until it reaches the measurement section of the flow chamber. The sample injection tube, at a
constant speed and within a certain period of time, pushes the sample fluid wrapped in the
sheath flow so that it travels steadily through the flow chamber for optical measurement. The
measurement channels are cleaned after measurement to restore them to their
pre-measurement status.
3.4.1.2 Measurement Process

The measurement process for the DIFF measurement module is as follows:
1. Dosing: Use the LS lyse syringe to add 1.4 ml of LYA-3 to the DIFF bath, before using the sample
probe to add 9ul of the blood sample in the DIFF bath for incubation. After a period of incubation,
LYA-2 is added for further incubation.
2. Mixing: Open Valve LV14 to generate air bubbles through the intermittent valve opening to mix
the sample well.
3. Measurement: Open Valve LV12 to aspirate the incubated sample from the DIFF bath using the
sheath flow syringe, then close Valve LV12 and open Valve LV10 and Valve LV11 so that the
sheath flow syringe can push the sheath fluid into the flow chamber, thereby forming a stable
sheath flow. Then push the sample into the flow chamber using the sample syringe. This is how
the sample, bundled in the sheath flow, enters the measurement section for measurement.
4. Cleaning: In preparation for the sample, open Valve LV12 and Valve LV13 to clean the sample
preparation tubing using the sheath flow syringe. Open Valve LV10, Valve LV11, and Valve LV18
while closing Valve LV22, and clean the flow chamber using the sheath flow syringe.
5. Waste discharge: Waste is discharged by opening Valve LV30 and Pump P2.
3.4.2 WBC/HGB Measurement Channel

 Measurement principles: Impedance method and colorimetric method
 Measurement parameters: WBC and HGB
 Graph information: WBC histogram
 LYA-1: Lyses the RBC and combines with hemoglobin.
 Diluent: This diluting and cleaning agent can provide an electrically conductive environment
and process cells and so on in bulk.
 The impedance method is used during WBC measurement: After the addition of LYA-1 lyse,
the red blood cells are lysed. Through the negative-pressure chamber (with a negative
pressure of -30Kpa), the sample is aspirated out of the WBC bath through the aperture. The
WBC particles generate electric pulses when traveling through the aperture, allowing the
WBC cells to be counted according to the number of pulses emitted.
 HGB is measured by colorimetry: Prior to the addition of the blood sample, the baseline
voltage of the diluent is first measured. Then the blood sample and lyse are mixed well for a
complete reaction so that the parameter voltage of the sample can be measured. HGB can
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3 Hydraulics System
then be calculated based on the local voltage and parameter voltage according to
Lambert-Beer's Law.

The measurement process for the WBC/HGB measurement module is as follows:
1. Dosing: The diluent syringe is first applied to add the diluent into the WBC bath, and then the
sample probe is used to add the blood sample into the WBC bath, where they are mixed evenly.
After aspirating the diluted sample for the first time, LYA-1 lyse is added into the WBC bath for
incubation.
the sample well.
3. Measurement: Open Valve LV27 to aspirate the sample out of the WBC bath through the
aperture by means of the negative-pressure chamber (with a negative pressure of -30Kpa). The
WBC particles generate electric pulses when traveling through the aperture, allowing the WBC
cells to be counted according to the number of pulses emitted.
4. Cleaning: To clean, open LV4 and LV8, and add the diluent into the WBC bath using the diluent
syringe.
5. Waste discharge: Waste is discharged by opening Valve LV25 (or Valve 21) and Pump P1.
3.4.3 RBC/PLT Measurement Module

 Measurement principles: Impedance method
 Measurement parameters: RBC and PLT
 Graph information: RBC histogram and PLT histogram
Diluent: This diluting and cleaning agent can provide an electrically conductive environment and
process cells and so on in bulk.
RBC and PLT measurement makes use of the impedance method: After the addition of LYA-1
lyse, the red blood cells are dissolved. Through the negative-pressure chamber (with a negative
pressure of -30Kpa), the sample is aspirated out of the WBC bath through the aperture. The RBC
and PLT particles generate electric pulses of various amplitudes when traveling through the
aperture, allowing them to be counted according to the number of pulses emitted.

The measurement process for the RBC/PLT measurement module is as follows:
1. Dosing: The diluent syringe is first applied to add the diluent into the RBC/PLT bath as the base
liquid, before using the sample probe to aspirate the sample diluted for the first time from the
WBC bath into the RBC/PLT bath.
the sample well.
14
3 Hydraulics System
3. Measurement: Open Valve LV28 to aspirate the sample out of the WBC bath through the
aperture by means of the negative-pressure chamber (with a negative pressure of -30Kpa). The
RBC and PLT particles generate electric pulses of various amplitudes when traveling through the
aperture, allowing the RBC and PLT cells to be counted according to the number of pulses
emitted.
4. Cleaning: To clean, open Valve LV4 and add the diluent into the RBC/PLT bath using the diluent
syringe.
5. Waste discharge: Waste is discharged by opening Valve LV26 and Pump P2.
3.4.4 Sampling and Blood-dispensing Module

This module consists of the sample probe, the sample syringe, the sampling tube, the swab, the
horizontal motor, and the vertical motor.
This is how the sampling and blood-dispensing module works:
Open Valve LV09 and aspirate the sample from the testing tube using the sample syringe→Clean
the outer wall of the sample probe→Move the sample probe to the WBC bath→Set aside a fixed
quantity of the blood sample→Move the sample probe upwards→ …until it reaches the DIFF
bath→Put aside a fixed amount of blood in the DIFF bath→Clean the outer and inner wall of the
sample probe→Aspirate out of the WBC bath a fixed amount of the sample that has been diluted
once→Clean the outer wall of the sample probe and move the sample probe to the RBC bath→Add
into the RBC bath the sample that has been diluted once→Clean the outer wall of the sample probe
to complete the entire process of sampling and blood dispensing.
In summary, this module works by aspirating the sample and dispensing a fixed amount of the
sample into the corresponding reaction baths, while cleaning the inner and outer walls of the sample
probe to prevent cross-contamination.
3.4.5 Power Supply and Waste Discharge Module

 Power supply module
By opening Valve LV29 and Pump P2 to establish a negative pressure of -30Kpa, this module
provides propulsion for the counting of the impedance channel.
 Waste disposal module
This module consists of the following five parts:
 Discharge of waste resulting from swab cleansing
Valve LV19 and Pump P1 are opened to discharge any waste resulting from using the swab
to clean the sample probe.
 Discharge of waste from the DIFF bath
Valve LV30 and Pump P2 are opened to discharge any waste from the DIFF bath.
 Discharge of waste from the WBC bath
Valve LV25 and Pump P1 are opened to discharge any waste from the WBC bath.
 Discharge of waste from the RBC/PLT bath
Valve LV26 and Pump P2 are opened to discharge any waste from the RBC/PLT bath.
 Discharge of waste from the impedance counting bath
15
3 Hydraulics System
After any waste from impedance counting (WBC counting and RBC/PLT counting) flows
through the aperture, and after any waste that results from cleaning the rear section of the
WBC and RBC/PLT baths flows into the negative-pressure chamber, Valve LV29 and Pump
P2 are opened for waste discharge.
3.4.6 Status Monitoring Module

This module involves:
 Monitoring of the diluent
Detection is done by way of a floating sensor; the float moves downwards the diluent level falls,
and an alarm is activated when it reaches empty.
 Monitoring of LYA-3
An optocoupler is used to monitor the LYA-3 level; as it gets lower, the optocoupler’s status
changes until an alarm is activated once there is no LYA-3 left.
 Monitoring of waste overflow
Waste overflow is done by way of a floating sensor; as waste levels rise, the float moves upward
until it eventually activates an alarm indicating that it is full.
 Monitoring of the sheath-flow pressure sensor
When the flow chamber or the sample probe is clogged, the sheath-flow pressure sensor detects
when the pressure is beyond the rated range. It then activates an alarm and puts the machine in
an emergency shut-down status.
3.4.7 Hydraulic Components
List of Hydraulic Components
No. Component Illustration Material Function description Remarks

No. No.
1 Sample 56010280A Collects blood samples in N/A

probe the testing tube and
dispenses a certain portion
of the sample to be placed
in the corresponding
counting bath
16
3 Hydraulics System

No. No.
2 Swab 56010068A Cleans the outer and inner N/A

walls of the sample probe
3 Two-way 24130010A On-off switch for fluid N/A

valve (L) control
4 Three-way 24130009A Directional hydraulic valve N/A

valve (L)
5 Two-way 24130002A On-off switch for fluid N/A

valve (S) control
6 Three-way 24130003A Directional hydraulic valve N/A

valve (S)
7 Pinch valve 24130006A On-off switch for fluid N/A

control
8 One-way 24130008A Pump inlet for air intake N/A

valve and prevention of leakage
9 Liquid pump 24010001A Discharges waste and N/A

creates a vacuum
10 Air pump 24010002A Builds up positive pressure N/A
11 Lyse syringe 20010040A Dispenses the lyse N/A

-LS
17
3 Hydraulics System

No. No.
12 Diluent 20010041A Located on the left side of N/A

syringe-DT the device, this syringe
dispenses the diluent.
13 Sheath flow 20010042A Located on the front side N/A

syringe-SH the device, this syringe is
used to create sheath flow
and clean the DIFF
channel.
14 Sample 20010043A This syringe pushes the N/A

syringe-ASP sample into the
measurement section of
the flow chamber for testing
15 DIFF bath 20010018A The sample undergoes N/A

reaction and incubation in
the DIFF channel.
16 Isolation 60010012A This chamber insulates the N/A

chamber liquid from the electric
circuits to prevent any
electrical interference.
17 Flow 20010021A This chamber is where the N/A

chamber sheath flow is formed and
optical measurement takes
place
18
3 Hydraulics System

No. No.
18 WBC/HGB 20010007A 1) This consists of the front N/A

bath bath and the rear bath, with
an aperture in between; the
sample flows from the front
bath through the aperture
to generate electric pulses
that facilitate WBC
counting;
2) The counting bath is
accompanied by a HGB
emission light and a HGB
signal receiving device for
HGB measurement.
19 RBC/PLT 20010008A This consists of the front N/A

bath bath and the rear bath, with
an aperture in between; the
sample flows from the front
bath through the aperture
to generate electric pulses
that facilitate RBC and PLT
counting.
21 Positive-pres 20010004A 1) This chamber builds the N/A

sure positive pressure needed
chamber for generating air bubbles
in the DIFF bath, the
WBC/HGB bath, and the
RBC/PLT bath;
2) It also builds the positive
pressure needed for
aperture backflush in the
counting bath.
22 Negative-pre 20010004A 1) This chamber builds the N/A

ssure negative pressure needed
chamber to drive
impedance-channel
counting;
3) It also builds the
negative pressure needed
to rinse the rear section of
the impedance counting
bath.
19
3 Hydraulics System

No. No.
23 Filter 53990018A Prevents small particles N/A

from blocking the
hydraulics system
24 Inline filter 53990036A Prevents impurities or N/A

particles from the hydraulic
system, which may result in
pump and/or valve failure.
List of Tube Types
No. Tube Illustration Material Inner Outer Features

Name No. Diameter Diameter
(mm) (mm)
1 Double-La 63010006A 2.0 3.5 Double-layered with

yered an erosion-resistant
Tube inner layer
2 EVA Tube 63010007A 1.0 3 Has good flexibility

and resistance to
bending and fatigue
3 Thin 50 63010008A 1.6 3.2 Soft Tube

Tube
4 Thick 50 63010009A 2.4 4 Soft Tube

Tube
5 3603 Tube 63010010A 3.2 6.4 A soft tube used for

the external inflow of
diluent and waste
discharge
6 MPF Tube 63010011A 2 3.5 A hard tube, resistant

to pressure and
deformation
20
3 Hydraulics System
No. Tube Illustration Material Inner Outer Features

Name No. Diameter Diameter
(mm) (mm)
7 1.5mm 63010013A 1.5 2.5 This tube is hard and

Teflon transparent, and is
Tube very resistant to
erosion
8 1.0mm 63010014A 1.0 1.68 This tube is hard and

Teflon transparent, and is
Tube very resistant to
erosion
9 STHT 63010001A 0.78 4 This tube is soft and

Tube can be connected to
hard tubes
10 Pharmed 63010002A 1.6 3.2 This is a soft tube with

Tube good resilience
11 Thin 1.6 63010003A 1.6 3.2 This is a soft tube for

mm (i.d.) pinch valves
Silicone
Tube
12 Thick 63010004A 1.6 4.8 This tube is soft and

1.6mm can be connected to
(i.d.) hard tubes
Silicone
Tube
13 3.2mm 63010005A 3.2 6.4 This tube is soft and

(i.d.) can be connected to
Silicone hard tubes; it can also
Tube be used as a
protective shell
14 TPU Tube 63010016A 2.4 4.8 A hard tube, resistant

to pressure and
deformation
15 2.0mm 63010020A 2 4 This tube is hard, has

(i.d.) good resistance to
Teflon erosion, and can be
Tube used as an aspiration
tube inside the bottle
of lyse
21
3 Hydraulics System
List of Connector Types
No. Connector Illustration Material Materi Features

Name No. al
1 T420-1 53050003 White T-type Connector for 1.6-2.4mm

Connector A nylon (i.d.) Tubing
2 K420-6005 53050005 PP 10-32 screw-thread connector for

Connector A 1.6-2.4mm (i.d.) tubing
3 S220-6005 53050008 PP, 1/4-28 screw-thread connector for

Connector A natural 1.6-2.4mm (i.d.) tubing
color
4 L420-1 53050010 White L-type Connector for 1.6-2.4mm

Connector A nylon (i.d.) tubing
5 Y230-1 53050011A White Y-type Connector for 2.4-3.2mm

Connector nylon (i.d.) tubing
6 Y420-1 53050012 White Y-type Connector for 1.6-2.4mm

Connector A nylon (i.d.) tubing
7 N430/420-1 53050013 White Thick-to-thin tube connector for

Connector A nylon 1.6-2.4mm configuration
8 N420-6005 53050014 PP, Barrel connector for 1.6-2.4mm

Connector A natural (i.d.) tubing
color
9 MTLL230-1Inte 53050016 White Integrated locking ring

grated locking A nylon
ring
10 MTLL007-2 53050045 Black Integrated locking ring

Integrated A nylon
locking ring
11 MTLL013-3 53050044 Red Integrated locking ring

Integrated A nylon
locking ring
22
3 Hydraulics System

Name No. al
12 MTLL007-4 53050046 Green Integrated locking ring

Integrated A nylon
locking ring
13 MTLL007-5 53050047 Blue Integrated locking ring

Integrated A nylon
locking ring
14 LNS-1 Locking 53050023 White Locking bolt for white panel

bolt for panel A nylon installation
installation
15 LNS-2 Locking 53050024 Black Locking bolt for black panel

installation
16 LNS-3 Locking 53050025 Red Locking bolt for red panel

installation
17 LNS-4 Locking 53050026 Green Locking bolt for green panel

installation
18 LNS-5 Locking 53050027 Blue Locking bolt for blue panel

installation
19 CCLR-1 53050028 White White-coded locking ring

Color-coded A nylon
locking ring
20 CCLR-2 53050029 Black Black-coded locking ring

Color-coded A nylon
locking ring
21 CCLR-3 53050030 Red Red-coded locking ring

Color-coded A nylon
locking ring
22 CCLR-4 53050031 Green Green-coded locking ring

Color-coded A nylon
locking ring
23
3 Hydraulics System

Name No. al
23 CCLR-5 53050032 Blue Blue-coded locking ring

Color-coded A nylon
locking ring
3.4.8 Main Measurement Modes and Procedures

The measurement modes of the analyzer include:
 Open-vial Whole-blood CBC+DIFF Mode
Whole-blood sample aspiration for CBC+DIFF measurement.
 Autoloader Whole-blood CBC+DIFF Mode
Whole-blood sample aspiration for CBC+DIFF measurement.
 Predilute CBC+DIFF Mode
Dilution is performed in vitro, and the diluted sample is then aspirated for CBC+DIFF
measurement.
 Whole-blood CBC Mode
Whole-blood sample aspiration for CBC measurement.
 Autoloader Whole-blood CBC Mode
Whole-blood sample aspiration for CBC measurement.
 Predilute CBC Mode
Dilution is performed in vitro, and the diluted sample is then aspirated for CBC measurement.
3.4.8.1 Dilution Procedure in Open-vial Whole-blood CBC+DIFF Mode

Dilution Procedure in Open-vial Whole-blood CBC+DIFF Mode:
Figure 3-1 Dilution Procedure in Open-vial Whole-blood CBC+DIFF Mode
Whole-blood
CBC+DIFF Mode
Sampling
DiscardIng Discarding
blood blood
Dispensing
Dispensing Diluted sample

LYA-3 LYA-2 LYA-1 Diluent Diluent
24
3 Hydraulics System
3.4.8.2 Dilution Procedure in Autoloader Whole-blood CBC+DIFF Mode

Dilution Procedure in Autoloader Whole-blood CBC+DIFF Mode:
Figure 3-2 Dilution Procedure in Whole-blood CBC+DIFF Mode
Whole-blood
CBC+DIFF Mode
Sampling
DiscardIng Discarding
blood blood
Dispensing
Dispensing Diluted sample

3.4.8.3 Dilution Procedure in Predilute CBC+DIFF Mode

Dilution Procedure in Predilute CBC+DIFF Mode:
Figure 3-3 Dilution Procedure in Whole-blood CBC+DIFF Mode
Blood Diluent
20ul 180ul
Predilute
CBC+DIFF mode 200ul dilution
sampling (Ratio1:10)
Diluted sample
Dispensing Dispensing

3.4.8.4 Dilution Procedure in Open-vial Whole-blood CBC Mode

Dilution Procedure in Open-vial Whole-blood CBC Mode:
Figure 3-4 Dilution Procedure in Whole-blood CBC Mode
Whole-blood CBC
mode sampling
Discarding blood Discarding blood
Diluted sample
Dispensing
WBC/HGB RBC/PLT
measurement measurement
module module
LYA-1 Diluent Diluent
25
3 Hydraulics System
3.4.8.5 Dilution Procedure in Autoloader Whole-blood CBC Mode

Dilution Procedure in Autoloader Whole-blood CBC Mode:
Figure 3-5 Dilution Procedure in Whole-blood CBC Mode
Whole-blood CBC
mode sampling
Discarding blood Discarding blood
Diluted sample
Dispensing
WBC/HGB RBC/PLT
module module
3.4.8.6 Dilution Procedure in Predilute CBC Mode

Dilution Procedure in Predilute CBC Mode:
Figure 3-6 Dilution Procedure in Predilute CBC Mode
Blood Diluent
20ul 180ul
Predilute CBC 200ul dilution

mode sampling (Ratio1:10)
DiscardIng DiscardIng
blood blood
Diluted sample
Dispensing
WBC/HGB RBC/PLT
module module
3.4.8.7 Measurement Procedure in Open-vial Whole-blood CBC+DIFF Mode

In open-vial whole-blood CBC+DIFF mode, a total of 9 time slots for sample measurement are set at
0~6s, 7~15s, 16~20s, 21~27s, 28~31s, 32~44s, 45~48s, 49~54, and 54~60s, respectively, during
each of which different steps are taken.
26
3 Hydraulics System
 Steps taken between 0~6s (Figure 3-7)

a. Aspirate the whole blood using the sample syringe.
b. Set the sample probe in the upper position for outer-wall cleaning.
Figure 3-7 Measurement Flowchart A (0~6s)
3 1 2:COM 1
2:IN T76
1:NC 1:OUT J12 T70 J11 J6 T79
2 T76
2 3:NO
J12 T78 J12 T70 J11 J8 T79
J3 T53 J10 J9 T14
J2 T29 T42-2
T70 T43
T1 T25-1 J3 T3 J3 J34 J35 J36
Flow
T2 LV5 LV6 T15
chamber
T33 T80 T80 T80
T23-1
T40 T70 LV7小阀 LV3 T12 T13 T13
T70 T70 T70 T70
T70 J1 LV2 LV1
T41 T29 T30 T80
T21-1 LV8 Diluent
LV11 T70 J3 T26 LV4 J12 T75 T75
LYA-3 LYA-2 LYA-1
T80 T4
T25 T2
LV12 T23 J1 T74
LV10 J1 J1 J1
T22-3 LV9 J12
T24 T29
T22-2 J3 Diluent
J3 T31-1 T80 Lyse T67
T21-2 LV13 syringe
T29
T67
valve
Sheath
T73 PP03
Flow Syringe
syringe T52
T52 T22
(100ul)
T9 T67 T67
T70 T42-1
T20 LV27 T80 T80 J5 T19 J78
J48
T73 LV28 J12
T81 T73 T73 T49 T67 LV17 T29
casing J46 J47 T50
LV18 RBC T80 LV20 T62 T66 T29
T29 T70 T29 T82 T80 T80 T80
T29
WBC
T80 T80
T51 waste
DIFF T29 T29 J5 T80 J5 J13 T16 T14
T47 T80 T82 T83 T67 T18
T54 T29 T29 T38 T57 T63
swab J3 J3 T83 J51 T70 T66
T70 T82 T10 T48 J3 J60
T29 J5 T70 J10
T69 T10 T48 T80
J13 J1 T29 T70 T29 T80 T70 liquid pump PP02
T29 Isolation T84 T50 T70 T90 J14
T9 T70 T29 T29 T84 T50 T70 J13 T18 J4
sample In-line T35 T48 T37 J3 chamber T59 T59 T11 LV16 T87 T17
T35 J12 J61
probe T9 filter T80 T29
T28 In-line J52 T11 In-line LV23 negative-
T62
Isolation T27 T11 LV15 filter T9 T11 pressure
J13
T57 LV22 filter T9 J13 T57 T57 chamber J5 Note：
chamber J12 J13 T70
J5 J13 T65
T57
T66 LV26 sensor
LV19 T9 J13 T29 T62
T58
T56 T66
T7 J5 J3
LV30 T3 J3 T22
T63 J5 T62 J5
T67
T64

a. The sampling assembly moves over the WBC bath and the sample probe moves into the
WBC bath.
b. The contaminated sample is removed from the tip of the sample probe from inserting it into
the WBC bath.
c. Drain the blood sample and diluent from the WBC/HGB bath.
d. Add the diluent into the WBC/HGB bath.
e. The sample probe dispenses the blood sample into the WBC bath while adding LYA-1 and
mixing the sample by swinging the probe or via air bubbles.
f. Move the sample probe to the upper position.
g. Drain the DIFF bath and clean it with LYA-3.
27
3 Hydraulics System
Figure 3-8 Measurement Flowchart B

3 1 2:COM 1
2:IN T76
1:NC 1:OUT J12 T70 J11 J6 T79
2 T76
2 3:NO
J12 T78 J12 T70 J11 J8 T79
J3 T53 J10 J9 T14
J2 T29 T42-2
T70 T43
T1 T25-1 J3 T3 J3 J34 J35 J36
Flow
T2 LV5 LV6 T15
chamber
T33 T80 T80 T80
T23-1
T40 T70 LV7 LV3 T12 T13 T13
T70 T70 T70 T70
T70 J1 LV2 LV1
T41 T29 T30 T80
T21-1 LV8 Diluent
LV11 T70 J3 T26 LV4 J12 T75 T75
LYA-3 LYA-2 LYA-1
T80 T4
T25 T2
LV12 T23 J1 T74
LV10 J1 J1 J1
T22-3 LV9 J12
T24 T29
T22-2 J3 Diluent
J3 T31-1 T80 Lyse T67
T21-2 LV13 syringe
T29
J1 J1 J1 (10ml) (2.5ml) One-way J5 T67
valve
Sheath
T73 PP03
Flow Syringe
syringe T52
T52 T22
(100ul)
T9 T67 T67
T70 T42-1
T20 LV27 T80 T80 J5 T19 J78
J48
T73 LV28 J12
T81 T73 T73 T49 T67 LV17 T29
casing J46 J47 T50
T82 positive-pressure
pipe T29 T49 T70 T35
T29 LV18 T82
RBC
T80 T80 LV20 T62 T66 T29
T29 T70 WBC T80 J5 LV24 J12
T29 T80 T80 T51 PP01 liquid pump waste
DIFF T29 T29 J5 T80 J5 J13 T16 T14
T47 T80 T82 T83 T67 T18
T54 T29 T29 T38 T57 T63
swab J3 J3 T83 J51 T70 T66
T70 T82 T10 T48 J3 J60
T29 J5 T70 J10
T69 T10 T48 T80
J13 J1 T29 T70 liquid pump PP02
T70 T29 T80
T84 T50 T70 T90 J14
T29 T70 T29 T29 T84 T50 T70 J13 T18 J4
T9 In-line Isolation T59 T11 LV16 T87 T17
T35 T48 T37 J3 T59 negative-
sample T35 J12 chamber J61
T9
filter
T80 T29
T28 J52 T11 LV23 pressure T62
probe
T27 In-line filter T11 LV15 In-line filter
T9
T11
chamber
J13 Isolation
T57 LV22 T9 J13 T57 T57 J5 Note：
chamber J12 J13 T70
J5 J13 T65
LV19 T31-2 LV21 T66 negative-pressure
T57
T66 LV26 sensor
T9 J13 T29 T62
T58
T56 T66
T7 J5 J3
LV30 T3 J3 T22
T63 J5 T62 J5
T67
T64

a. The sampling assembly moves over the DIFF bath and the sample probe moves into the
DIFF bath.
b. The sample probe dispenses the blood sample into the DIFF bath while adding LYA-3 and
mixing the sample through with air bubbles.
c. Move the sample probe to the upper position and clean its inner and outer walls after the
blood is dispensed.
Figure 3-9 Measurement Flowchart C
3 1 2:COM 1
2:IN T76
1:NC 1:OUT J12 T70 J11 J6 T79
2 T76
2 3:NO
J12 T78 J12 T70 J11 J8 T79
J3 T53 J10 J9 T14
J2 T29 T42-2
T70 T43
T1 T25-1 J3 T3 J3 J34 J35 J36
Flow
T2 LV5 LV6 T15
chamber
T33 T80 T80 T80
T23-1
T40 T70 LV7 LV3 T12 T13 T13
T70 T70 T70 T70
T70 J1 LV2 LV1
T41 T29 T30 T80
T21-1 LV8 Diluent
LV11 T70 J3 T26 LV4 J12 T75 T75
LYA-3 LYA-2 LYA-1
T80 T4
T25 T2
LV12 T23 J1 T74
LV10 J1 J1 J1
T22-3 LV9 J12
T24 T29
T22-2 J3 Diluent
J3 T31-1 T80 Lyse T67
T21-2 LV13 syringe
T29
J1 J1 J1 (10ml) (2.5ml) J5
One-way T67
T73 PP03 valve
Sheath T29 positive-pressure T39
Flow Syringe
syringe T52
T52 T22
(100ul)
T9 T67 T67
T70 T42-1
T20 LV27 T80 T80 J5 T19 J78
J48
T73 LV28 J12
T81 T73 T73 T49 T67 LV17 T29
casing J46 J47 T50
T29 LV18 T82
RBC
T80 T80 LV20 T62 T66 T29
T29 T70 T80
WBC J5 LV24 J12
DIFF T29 T29 J5 T80 J5 J13 T16 T14
T47 T80 T82 T83 T67 T18
T54 T29 T29 T38 T57 T63
swab J3 J3 T83 J51 T70 T66
T70 T82 T10 T48 J3 J60
T29 J5 T70 J10
J13 J1 T29 T70 T29 T80 T70 PP02 T90
T29 T70 Isolation T84 T50 T70 T84 T50 T70 J14
T9 In-line T29 T29 J13 T18 J4
sample
T35 T48 T37 J3 chamber T59 T59 T11 LV16 T87 T17
probe filter T35 J12 Negative-
T29 J52 T11 In-line J61 LV23
T9 T80 T28 In-line pressure T62
Isolation T27 T11 LV15 filter T9 T11
chamber
J13
chamber T57 LV22 filter T9 J13 T57 T57 J5 Note：
J12 J13 T70
T55 T9 J13 T65 T2 T9 T2 LV-Liquid valve； PP-pump；
In-line filter T9 J13 T29 T50
J5 J13 T65
T57
LV19 T9 T66 LV26 sensor
J13 T29 T62
T58
T56 T66
T7 J5 J3
LV30 T3 J3 T22
T63 J5 T62 J5
T67
T64
28
3 Hydraulics System

a. Insert the sample probe into the WBC/HGB bath to aspirate the diluted sample.
b. Move the sample probe to the upper position.
c. Add the LYA-1 lyse into the WBC/HGB bath and mix through with air bubbles.
d. Move the sample probe over the RBC/PLT bath, and then insert it into the RBC/PLT bath.
e. Add LYA-2 into the DIFF bath.
f. Use the sheath flow syringe to aspirate the sample from the DIFF bath to the 3-way
connector at the lower end of the flow chamber.
g. A pressure of -30Kpa is created in the negative-pressure chamber.
Figure 3-10 Measurement Flowchart D
3 1 2:COM 1
2:IN T76
1:NC 1:OUT J12 T70 J11 J6 T79
2 T76
2 3:NO
J12 T78 J12 T70 J11 J8 T79
J3 T53 J10 J9 T14
J2 T29 T42-2
T70 T43
T1 T25-1 J3 T3 J3 J34 J35 J36
Flow
T2 LV5 LV6 T15
chamber
T33 T80 T80 T80
T23-1
T40 T70 LV7 LV3 T12 T13 T13
T70 T70 T70 T70
T70 J1 LV2 LV1
T41 T29 T30 T80
T21-1 LV8 Diluent
LV11 T70 J3 T26 LV4 J12 T75 T75
LYA-3 LYA-2 LYA-1
T80 T4
T25 T2
LV12 T23 J1 T74
LV10 J1 J1 J1
T22-3 LV9 J12
T24 T29
T22-2 J3 Diluent
J3 T31-1 T80 Lyse T67
T21-2 LV13 syringe
T29
T73 PP03 valve
Flow Syringe
syringe T52
T52 T22
(100ul)
T9 T67 T67
T70 T42-1
T20 LV27 T80 T80 J5 T19 J78
J48
T73 LV28 J12
T81 T73 T73 T49 T67 LV17 T29
casing J46 J47 T50
T29 LV18 T82
RBC
T80 T80 LV20 T62 T66 T29
T29 T70 WBCT80 J5 LV24 J12 PP01 liquid pump
T29 T80 T80 T51 waste
DIFF T29 T29 J5 T80 J5 J13 T16 T14
T47 T80 T82 T83 T67 T18
T54 T29 T29 T38 T57 T63
J3 J3 T83 J51 T70 T66
swab T70 T82 T10 T48 J3 J60
T29 J5 T70 J10
J13 J1 T29 T70 PP02
T70 T29 Isolation
T80
T84 T50 T70 T90 J14
T29 T70 T29 T29 T84 T50 T70 J13 T18 J4
T9 In-line chamber T59 T11 LV16 T87 T17
sample T35 T48 T37 J3 T59
T35 J12 J61
probe T9
filter
T80 T29
T62
J13
chamber T57 LV22 filter T9 J13 T57 T57 chamber J5 Note：
J12 J13 T70
J5 J13 T65
T57
T66 LV26 sensor
LV19 T9 J13 T29 T62
T58
T56 T66
T7 J5 J3
LV30 T3 J3 T22
T63 J5 T62 J5
T67
T64

a. Add the diluted sample into the RBC/PLT bath once with the sample probe, and mix well
using the air bubbles.
b. Move the sample probe upward into the upper position and clean its outer wall.
c. Push the diluent into the flow chamber with the sheath flow syringe, thereby forming a steady
sheath flow.
d. The sample syringe begins to push the sample into the flow chamber.
e. DIFF measurement is in progress.
29
3 Hydraulics System
Figure 3-11 Measurement Flowchart E

3 1 2:COM 1
2:IN T76
1:NC 1:OUT J12 T70 J11 J6 T79
2 T76
2 3:NO
J12 T78 J12 T70 J11 J8 T79
J3 T53 J10 J9 T14
J2 T29 T42-2
T70 T43
T1 T25-1 J3 T3 J3 J34 J35 J36
Flow
T2 LV5 LV6 T15
chamber
T33 T80 T80 T80
T23-1
T40 T70 LV7 LV3 T12 T13 T13
T70 T70 T70 T70
T70 J1 LV2 LV1
T41 T29 T30 T80
T21-1 LV8 Diluent
LV11 T70 J3 T26 LV4 J12 T75 T75
LYA-3 LYA-2 LYA-1
T80 T4
T25 T2
LV12 T23 J1 T74
LV10 J1 J1 J1
T22-3 LV9 J12
T24 T29
T22-1 T47 T44
T22-2 J3 Air pump Air Filter
J3 T31-1 T80 Diluent Lyse
T32 T67
syringe syringe T88 J5 T80 J13 T57 T70 T70
T21-2 LV13 T29
T67
valve
Sheath
T73 PP03
Flow Syringe
syringe T52
T52 T22
(100ul)
T9 T67 T67
T70 T42-1
T20 LV27 T80 T80 J5 T19 J78
J48
T73 LV28 J12
T81 T73 T73 T49 T67 LV17 T29
casing J46 J47 T50
pipe T29 T49 T82
T70 T35 positive-pressure
T29 LV18 T82
RBC
T80 T80 LV20 T62 T66 T29
T29 T70 T80
WBC J5 LV24
T29 T80 T80 T51
J12 PP01 liquid pump waste
DIFF T29 T29 J5 T80 J5 J13 T16 T14
T47 T80 T82 T83 T67 T18
T54 T29 T29 T38 T57 T63
swab J3 J3 T83 J51 T70 T66
T70 T82 T10 J3 J60
T29 T48 J5 T70 J10
T69 T10 T48 T80
J13 J1 T29 T70 T29 T70 liquid pump PP02
T29 Isolation T80 T84 T50 T70 T90 J14
T9 In-line T70 T29 T29 T84 T50 T70 J13 T18 J4
T35 T48 T37 J3 chamber T59 T59 T11 LV16 T87 T17
T35 J12 J61
sample T9
filter
T80 T29
T62
probe J13
J12 J13 T70
J5 J13 T65
T57
T66 LV26 sensor
LV19 T9 J13
T58
T29 T62
T56 T66
T7 J5 J3
LV30 T3 J3 T22
T63 J5 T62 J5
T67
T64

a. The WBC channel starts measuring and then finishes measuring.
b. DIFF-channel measurement is complete.
c. RBC-channel measurement begins.
Figure 3-12 Measurement Flowchart F
3 1 2:COM 1
2:IN T76
1:NC 1:OUT J12 T70 J11 J6 T79
2 T76
2 3:NO
J12 T78 J12 T70 J11 J8 T79
J3 T53 J10 J9 T14
J2 T29 T42-2
T70 T43
T1 T25-1 J3 T3 J3 J34 J35 J36
Flow
T2 LV5 LV6 T15
chamber
T33 T80 T80 T80
T23-1
T40 T70 LV7 LV3 T12 T13 T13
T70 T70 T70 T70
T70 J1 LV2 LV1
T41 T29 T30 T80
T21-1 LV8 Diluent
LV11 T70 J3 T26 LV4 J12 T75 T75
LYA-3 LYA-2 LYA-1
T80 T4
T25 T2
LV12 T23 J1 T74
LV10 J1 J1 J1
T22-3 LV9 J12
T24 T29
T22-2 J3 Diluent
J3 T31-1 T80 Lyse T67
T21-2 LV13 syringe
T29
T67
valve
Sheath
T73 PP03
Flow Syringe
syringe T52
T52 T22
(100ul)
T9 T67 T67
T70 T42-1
T20 LV27 T80 T80 J5 T19 J78
J48
T73 LV28 J12
T81 T73 T73 T49 T67 LV17 T29
casing J46 J47 T50
pipe T29 T49 T82
T70 T35 positive-pressure
T29 LV18 T82
RBC
T80 T80 LV20 T62 T66 T29
T29 T70 T80 J5 LV24
T29
WBC
T80 T80 T51
J12 PP01 liquid pump waste
DIFF T29 T29 J5 T80 J5 J13 T16 T14
T47 T80 T82 T83 T67 T18
T54 T29 T29 T38 T57 T63
J3 J3 T83 J51 T70 T66
swab T70 T82 T10 T48 J3 J60
T29 J5 T70 J10
T69 T10 T48 T80
T9 T70 T29 T29 T84 T50 T70 J13 T18 J4
In-line T35 T48 T37 J3 chamber T59 T59 T11 LV16 T87 T17
sample filter T35 J12 J61 negative-
probe T9 T80 T29
J13 chamber T57 LV22 filter T9 J13 T57 T57 chamber
J5 Note：
J12 J13 T70
J5 J13 T65
T57
T66 LV26 sensor
T9 J13 T29 T62
T58
T56 T66
T7 J5 J3
LV30 T3 J3 T22
T63 J5 T62 J5
T67
T64
30
3 Hydraulics System

a. RBC/PLT-channel measurement is complete.
b. DIFF channel completes the cleaning of the sample preparation tubing.
c. Drain the WBC/HGB bath.
d. Add the diluent into the WBC/HGB bath for cleansing.
Figure 3-13 Measurement Flowchart G
3 1 2:COM 1
2:IN T76
1:NC 1:OUT J12 T70 J11 J6 T79
2 T76
2 3:NO
J12 T78 J12 T70 J11 J8 T79
J3 T53 J10 J9 T14
J2 T29 T42-2
T70 T43
T1 T25-1 J3 T3 J3 J34 J35 J36
Flow
T2 LV5 LV6 T15
chamber
T33 T80 T80 T80
T23-1
T40 T70 LV7 LV3 T12 T13 T13
T70 T70 T70 T70
T70 J1 LV2 LV1
T41 T29 T30 T80
T21-1 LV8 Diluent
LV11 T70 J3 T26 LV4 J12 T75 T75
LYA-3 LYA-2 LYA-1
T80 T4
T25 T2
LV12 T23 J1 T74
LV10 J1 J1 J1
T22-3 LV9 J12
T24 T29
T22-1 T47 T44
T22-2 J3 Diluent
Air pump Air Filter
J3 T31-1 T80 Lyse T67
T21-2 LV13 syringe
T29
T73 PP03 valve
Flow Syringe
syringe T52
T52 T22
(100ul)
T9 T67 T67
T70 T42-1
T20 LV27 T80 T80 J5 T19 J78
J48
T73 LV28 J12
T81 T73 T73 T49 T67 LV17 T29
casing J46 J47 T50 positive-pressure
LV18 RBC T80 LV20 T62 T66 T29
T29 T70 T29 T82 T80 T80 T80
T29
WBC
T80 T80
T51 waste
DIFF T29 T29 J5 T80 J5 J13 T16 T14
T47 T80 T82 T83 T67 T18
T54 T29 T29 T38 T57 T63
swab J3 J3 T83 J51 T70 T66
T70 T82 T10 T48 J3 J60
T29 J5 T70 J10
T69 T10 T48 T80
J13 J1 T29 T70 liquid pump PP02
T70 T29 T80
T84 T50 T70 T90 J14
T29 T70 T29 T29 Isolation T84 T50 T70 J13 T18 J4
T9 In-line T59 T11 LV16 T87 T17
T35 T48 T37 J3 chamber T59 negative-
T35 J12 J61
sample T9 filter T80 T29
LV15 filter T9 T11
filter T9 J13 T11
probe Isolation T27 chamber
J13
chamber T57 LV22 T57 T57 J5 Note：
J12 J13 T70
J5 J13 T65
T57
T66 LV26 sensor
LV19 T9 J13
T58
T29 T62
T56 T66
T7 J5 J3
LV30 T3 J3 T22
T63 J5 T62 J5
T67
T64
31
3 Hydraulics System

a. DIFF channel performs the cleaning of the sample preparation tubing again.
b. Zap the WBC/HGB bath.
c. Zap the RBC/PLT bath.
d. Drain the WBC/HGB bath.
e. Drain the RBC/PLT bath.
f. Add the base solution into the WBC/HGB bath.
g. Restore the sampling assembly to measurement preparation status.
Figure 3-14 Measurement Flowchart H
3 1 2:COM 1
2:IN T76
1:NC 1:OUT J12 T70 J11 J6 T79
2 T76
2 3:NO
J12 T78 J12 T70 J11 J8 T79
J3 T53 J10 J9 T14
J2 T29 T42-2
T70 T43
T1 T25-1 J3 T3 J3 J34 J35 J36
Flow
T2 LV5 LV6 T15
chamber
T33 T80 T80 T80
T23-1
T40 T70 LV7 LV3 T12 T13 T13
T70 T70 T70 T70
T70 J1 LV2 LV1
T41 T29 T30 T80
T21-1 LV8 Diluent
LV11 T70 J3 T26 LV4 J12 T75 T75
LYA-3 LYA-2 LYA-1
T80 T4
T25 T2
LV12 T23 J1 T74
LV10 J1 J1 J1
T22-3 LV9 J12
T24 T29
T22-2 J3 Diluent
J3 T31-1 T80 Lyse T67
T21-2 LV13 syringe
T29
J1 J1 J1 (10ml) (2.5ml) J5
One-way T67
T73 PP03 valve
Flow Syringe
syringe T52
T52 T22
(100ul)
T9 T67 T67
T70 T42-1
T20 LV27 T80 T80 J5 T19 J78
J48
T73 LV28 J12
T81 T73 T73 T49 T67 LV17 T29
casing J46 J47 T50
pipe T82 T35 positive-pressure
T9 T35 T29 T49 T85 T70
T35 T80 chamber
T29 LV18 T82
RBC
T80 T80 LV20 T62 T66 T29
T29 T70 WBC T80 J5 LV24 J12
DIFF T29 T29 J5 T80 J5 J13 T16 T14
T47 T80 T82 T83 T67 T18
T54 T29 T29 T38 T57 T63
swab J3 J3 T83 J51 T70 T66
T70 T82 T10 T48 J3 J60
T29 J5 T70 J10
T69 T10 T48 T80
T9 T70 T29 T29 T84 T50 T70 J13 T18 J4
sample
In-line T35 T48 T37 J3 chamber T59 T59 T11 LV16 T87 T17
T35 J12 J61 negative-
probe T9
filter
T80 T29
T28 In-line J52 T11 In-line LV23 T62
pressure
J13
J12 J13 T70
J5 J13 T65
T57
T66 LV26 sensor
T9 J13 T29 T62
T58
T56 T66
T7 J5 J3
LV30 T3 J3 T22
T63 J5 T62 J5
T67
T64

a. Add the base solution into the DIFF bath.
b. Add the base solution into the RBC bath.
c. Clean the rear baths of the WBC/HGB and RBC/PLT baths.
d. The sample probe moves into the blood-sampling position at about 58s.
e. Release the pressure on the positive-pressure chamber and negative-pressure chamber.
32
3 Hydraulics System
Figure 3-15 Measurement Flowchart I

3 1 2:COM 1
2:IN T76
1:NC 1:OUT J12 T70 J11 J6 T79
2 T76
2 3:NO
J12 T78 J12 T70 J11 J8 T79
J3 T53 J10 J9 T14
J2 T29 T42-2
T70 T43
T1 T25-1 J3 T3 J3 J34 J35 J36
Flow
T2 LV5 LV6 T15
chamber
T33 T80 T80 T80
T23-1
T40 T70 LV7 LV3 T12 T13 T13
T70 T70 T70 T70
T70 J1 LV2 LV1
T41 T29 T30 T80
T21-1 LV8 Diluent
LV11 T70 J3 T26 LV4 J12 T75 T75
LYA-3 LYA-2 LYA-1
T80 T4
T25 T2
LV12 T23 J1 T74
LV10 J1 J1 J1
T22-3 LV9 J12
T24 T29
T22-2 J3 Diluent
J3 T31-1 T80 Lyse T67
T21-2 LV13 syringe
T29
T67
valve
Sheath
T73 PP03
Flow Syringe
syringe T52
T52 T22
(100ul)
T9 T67 T67
T70 T42-1
T20 LV27 T80 T80 J5 T19 J78
J48
T73 LV28 J12
T81 T73 T73 T49 T67 LV17 T29
casing J46 J47 T50
T9 T35 T35 T85 T70
LV18 RBC T80 LV20 T62 T66 T29
chamber
T29 T70 T29 T82 T80 T80 T80
T29
WBC
T80 T80
T51 waste
DIFF T29 T29 J5 T80 J5 J13 T16 T14
T47 T80 T82 T83 T67 T18
T54 T29 T29 T38 T57 T63
swab J3 J3 T83 J51 T70 T66
T70 T82 T10 T48 J3 J60
T29 J5 T70 J10
T69 T10 T48 T80
J13 J1 T29 T70 T29 T70 liquid pump PP02
T29 T70 Isolation T80 T84 T50 T70 T90 J14
T9 In-line T29 T29 T84 T50 T70 J13 T18 J4
sample T35 T48 T37 J3 chamber T59 T59 T11 LV16 T87 T17
T35 J12 J61 negative-
probe T9
filter T80 T29
T28 In-line J52 T11 In-line LV23 T62
Isolation
T27 T11 LV15 filter T9 T11 pressure
J13 chamber T57 LV22 filter T9 J13 T57 T57 chamber J5 Note：
J12 J13 T70
J5 J13 T65
T57
T66 LV26 sensor
LV19 T9 J13
T58
T29 T62
T56 T66
T7 J5 J3
LV30 T3 J3 T22
T63 J5 T62 J5
T67
T64
3.4.8.8 Measurement Procedure in Autoloader Whole-blood CBC+DIFF Mode

The measurement procedure in autoloader CBC+DIFF mode is quite similar to that in whole-blood
CBC+DIFF mode. For details, see "3.4.8.7 Measurement Procedure in Open-vial Whole-blood
CBC+DIFF Mode".
3.4.8.9 Measurement Procedure in Predilute CBC+DIFF Mode

The measurement procedure in predilute CBC+DIFF mode is quite similar to that in whole-blood
CBC+DIFF mode; the only difference is that the sample used in the former mode involves blood
samples with a dilution ratio of 1/10, whereas the whole-blood mode uses undiluted venous blood.
Refer to "3.4.8.7 Measurement Procedure in Open-vial Whole-blood CBC+DIFF Mode" for a detailed
description of the relevant procedures.
3.4.8.10 Measurement Procedure in Open-vial Whole-blood CBC Mode

Except for when there is no DIFF-channel measurement procedure, the Open-vial whole-blood CBC
measurement procedure is identical to the whole-blood CBC+DIFF measurement procedure. Refer
to "3.4.8.7 Measurement Procedure in Open-vial Whole-blood CBC+DIFF Mode" for a detailed
3.4.8.11 Measurement Procedure in Autoloader Whole-blood CBC Mode

Except for when there is no DIFF-channel measurement procedure, the autoloader whole-blood
33
3 Hydraulics System
CBC measurement procedure is identical to the whole-blood CBC+DIFF measurement procedure.

3.4.8.12 Measurement Procedure in Predilute CBC Mode

The counting sequence in the predilute CBC mode is quite similar to that in whole-blood CBC+DIFF
mode; the only difference is that the sample used in the former mode involves blood samples with an
in-vitro dilution factor of 1/10, whereas the whole-blood mode uses undiluted venous blood.
3.5 Hydraulics Maintenance
3.5.1 Swab Cleaning and Maintenance

In the case of serious device contamination, our service team can provide on-site service either
regularly or by request. The swab requires cleaning and maintenance to get rid of any contamination
found on its lower surface.
The operating procedure as follows: Shut down the device and disassemble the swab. A certain
amount of probe cleanser is diluted with the diluent into a 1:3 (probe cleanser to diluent) solution.
Repeatedly scrub the bottom end of the swab and the inside of its lower aperture with a Q-tip
dampened with the diluted probe cleanser. After scrubbing, use another clean Q-tip dampened with
pure diluent to clean away the probe cleanser residue at the bottom end of the swab and the inside
of its lower aperture. (See Figure 3-16).
Figure 3-16 Cleaning the swab
After cleaning and reassembling the swab (and ensuring that the inflow/outflow interface tubing is
connected correctly), turn on the device and go to "Service > Clean". Double-click the "Sample
Probe" icon to perform the operation before performing cleaning and maintenance on the swab. See
Figure 3-17
3.5.2 Cleaning and Maintenance of the WBC Bath

Log onto the operation interface of the software, select "Service > Clean", and double-click the "WBC
Bath" icon. See Figure 3-17.
34
3 Hydraulics System
Figure 3-17 Cleaning and maintenance interface
3.5.3 Cleaning and Maintenance of the RBC Bath

Log onto the operation interface of the software, select "Service > Clean", and double-click the "RBC
Bath" icon. See Figure 3-17.
3.5.4 Cleaning and Maintenance of the DIFF Bath

In the case of serious device contamination, our service team can provide on-site service either
regularly or by request so help clean and maintain the DIFF bath.
Scrub around and the top of the inner aperture of the DIFF bath using a Q-tip dampened with the
probe cleanser diluted by the diluent (with a probe cleanser to diluent ratio of 1:3--See Figure 3-18).
After cleaning with the probe cleanser, use another clean Q-tip dampened with pure diluent to clean
away any probe cleanser residue.
Figure 3-18 Cleaning the DIFF bath
After cleaning, go to "Service > Clean" and double-click the " DIFF bath" icon, to finish cleaning and
maintaining the DIFF bath.
35
3 Hydraulics System
3.6 Troubleshooting Common Hydraulics Problems
3.6.1 Commonly Used Equipment and Tools

Parameter Illustration Usage Remarks
1. Used to contain liquid after a tube is

pulled out;
small sample cup 2. Used as a diluent container; N/A
3. The ratio of probe cleanser to diluent is
1:3.
1. Used to manually unclog the WBC and

plastic syringe RBC channels;
(disposal syringe 2. Used to manually unclog the flow N/A
with no pillow) chamber;
3. Used to inject other liquids.
Barrel connector tubing connection N/A
1.5mm Teflon Used to connect tubing and the plastic

N/A
Tube syringe
1. Used to scrub and clean the swab;

2. Used to scrub and clean the DIFF
Q-tips bath; N/A
3. Used for other scrubbing and cleaning
tasks.
3.6.2 Inspection and Troubleshooting of Valve Clogging

To prevent the possibility of liquid flowing onto the base plate when the tube connected to the valve is
pulled out, the tube pulled out needs to be placed into the small sample cup, and tissue paper should
be used as a pad to prop up any affected components. The sample cup should be removed once the
liquid has stopped flowing.
Disassemble the valve. Open the valve cap to identify any clogging caused by impurities. After the
impurities have been removed, place the components in their original positions. (Note that the parts
with instructions are kept on the same side during installation, as shown in Figure 3-21~ Figure 3-23.)
Then remove the tissue paper and scrub and clean the bottom.
36
3 Hydraulics System
Figure 3-19 3-way valve (L) Figure 3-20 2-way valve (L)
2,1,3 port
Figure 3-21 Installation of the large valve
Figure 3-22 Installation of the 3-way valve (L) Figure 3-23 Installation of the 2-way
valve (S)
3.6.3 Liquid Pump Clogging: Inspection and Troubleshooting

1. Pull out the tubing and place it into the small sample cup. Then prop up any relevant parts with
tissue paper and disassemble the pump. Use the Phillips screwdriver to open the pump cap to
observe any clogging resulting from impurities, as shown in Figure 3-24.
37
3 Hydraulics System
Figure 3-24 Pump cap
check
2. Remove the impurities and place the components in their original positions. Make sure to align
the two marked lines correctly while installing (as shown in Figure 3-25).
Figure 3-25 Installing the marked lines
aligned
3. When reassembling the pump, make sure the liquid inlet and outlet are connected to the correct
tubing (e.g. Figure 3-26).
Figure 3-26 Symbols for inflow and outflow direction
OUT
IN
3.6.4 Aperture Clogging of the WBC Channel

Aperture clogging can be resolved by implementing the procedure for cleaning the WBC bath and
using the aperture backflush.
38
3 Hydraulics System
1. Go to the "Service > Clean" screen in the operations interface, and double-click the "WBC Bath"
icon.
2. Go to the "Service > Maintain" screen, and double-click the "Flush Aperture" and then the "Zap
Aperture" icon.
3. After you are done, click the Remove Error button to check if the aperture has been unclogged.
If clogging persists, the probe cleanser needs to be manually pushed through to clean the
WBC-channel aperture, and the following steps should be taken:
1. After obtaining permission from qualified service personnel, first drain the WBC bath (the liquid
can also be aspirated dry manually using a plastic syringe or other tools): Go to the "Service >
Maintain" screen, double-click Empty WBC Bath (note that a prompt box will pop up for
confirmation after draining; do not click the OK button, otherwise the WBC bath will be refilled
with liquid).
2. Pull out the tubing connected to the WBC outflow tube and connect the plastic syringe filled with
the diluted probe cleanser (with a ratio of probe cleanser to diluent of 1:3) to the WBC outflow
tube (as shown in Figure 3-27). Pushing the plastic syringe back and forth will facilitate the
repeated flushing of the aperture with the probe cleanser. Apply proper force while pushing to
prevent the tube from dropping and spilling (you can hold the tube with your hand to keep it in
place).
Figure 3-27 Cleaning the WBC-bath aperture
3. Flush back and forth ten times, and then let it settle for 3 minutes. Afterward, flush it three more
times with a syringe containing the pure diluent using the aforementioned method.
4. After the diluent cleaning, drain the liquid inside the WBC bath and reconnect the original tubing.
To drain the WBC bath by interface operation, click the OK button in the prompt confirmation box
after draining, and then click Remove Error to see if the clogging problem is resolved.
5. If aperture clogging persists, redo the above procedures until the problem has been resolved.
3.6.5 Aperture Clogging of the RBC Channel

The solution is identical to "3.6.4 Aperture Clogging of the WBC Channel", except that the target
object is the RBC bath.
39
3 Hydraulics System
3.6.6 Clogging of the Flow Chamber/DIFF Probe

Manually push the probe cleanser to clean the flow chamber/DIFF probe:
1. Pull out the tube connected to Valve 18 from the flow chamber (while making sure that the
connecting tube has been pulled out correctly). Put the tube into the small sample cup, which is
used to hold the residue liquid flowing from the tube. The small sample cup can be removed
once no more residue is flowing (as shown in Figure 3-28).
Figure 3-28 Flow chart 1 of unclogging the flow-chamber (A)
Pull out the

tube here
User the small
sample cup to hold
the residue liquid
from the tube
pulled out.
2. Set the small sample cup under the pulled out connector. Once no more residue liquid flowage
can be detected, connect the connector to the silicone tube using the plastic syringe (as shown
in Figure 3-29).
Figure 3-29 Flow chart 2 of unclogging the flow-chamber (B)
Pull out this

connector
3. Connect the tube pulled out in Step 1 to a 1.5 mm Teflon tube about 100mm long using a barrel
connector and the silicone tube. Put the 1.5mm Teflon tube into the probe cleanser with a dilution
ratio of 1:3 (probe cleanser to diluent) below the surface of the liquid (as shown in Figure 3-30).
40
3 Hydraulics System
Figure 3-30 Flow chart 3 of unclogging the flow-chamber (C)
4. Pull the plastic syringe in Step 2 by hand and aspirate the probe cleanser into the flow chamber.
If you encounter a great deal of resistance while doing this, the plastic syringe will have to be
drawn until the probe cleanser level inside the small sample cup drops. Then you can let go and
begin to soak the probe cleanser.
5. After soaking it for five minutes or so, restore the original tubing and click the “Remove Error”
button to see if the problem has been resolved. If the problem persists, keep taking the
aforementioned steps until the problem has been resolved.
Figure 3-31 Restoration of the flow chamber after it is unclogged
41
3 Hydraulics System
3.6.7 Overflowing of the WBC Bath

First wipe off the liquid with tissue paper, and perform the following troubleshooting steps:
1. Check if Pump 1 is clogged.
Follow the steps specified in "3.6.3 Liquid Pump Clogging: Inspection and Troubleshooting" to
inspect and resolve any clogging of the liquid pump. After troubleshooting, run the CBC+DIFF
counting procedures to see if the problem has been resolved.
If the problem has been resolved, end the procedure; if the problem persists, continue to perform
the following steps:
2. Check if Valve 25 is clogged.
Follow the steps specified in "3.6.2 Inspection and Troubleshooting of Valve Clogging" to inspect
and resolve any clogging of Valve 25. After the troubleshooting, run the CBC+DIFF counting
procedures to see if the problem has been resolved.
3. Check if there are any kinks or damage along the tubing.
Check if there are any kinks or damage along the tubing, as highlighted in red in Figure 3-32, and
replace the corresponding tubing if the problem persists. After the troubleshooting, run the
CBC+DIFF counting to see if the problem has been resolved.
Figure 3-32 WBC tubing for fluid discharge
4. Check whether or not the isolation chamber is airtight.
After obtaining permission from qualified service personnel, first drain the WBC bath (the liquid
can also be aspirated dry manually using a plastic syringe or other tools): Go to the "Service >
Maintain" screen, double-click the "drain the WBC bath" button (note that a prompt box will pop
up for confirmation after draining; do not press the OK key, otherwise the WBC bath will be
refilled with liquid).
Disassemble the isolation chamber below the WBC bath and pull out the corresponding tubing.
Use the thick No. 50 tube or 1.6mm (i.d.) silicone tube to attach the two connectors to the side of
the isolation chamber, plugging both apertures. Then attach the plastic syringe to the connector
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3 Hydraulics System
above the isolation chamber using a 1.6mm (i.d.) silicone tube. Push the plastic syringe to
pressurize the isolation chamber, and hold the syringe steady for 30 seconds to check for any
appearance of air bubbles. If any air bubbles occur, the isolation chamber needs to be replaced.
Figure 3-33 Checking whether or not the isolation chamber is airtight
5. After inspection, the components and tubing need to be restored to their original locations. Click
on the OK key in the confirmation box that pops up after the counting bath is drained by opening
the user interface.
If the liquid level in the WBC bath is found to be too high, immediately shut down the device to
prevent any further overflowing.
3.6.8 Overflowing of the RBC Bath

The troubleshooting procedure is the same as that in "3.6.7 Overflowing of the WBC Bath"; the only
difference is that the pump to be inspected is Pump 2, and the valve to be inspected is Valve 26. The
tubing for inspection is shown in Figure 3-34, and the isolation chamber to be inspected is the one
below the DIFF bath.
Figure 3-34 RBC tubing of liquid discharge
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3 Hydraulics System
3.6.9 Overflowing of the DIFF bath

The troubleshooting procedure is the same as that in “3.6.7 Overflowing of the WBC Bath”; the only
difference is that the pump to be inspected is Pump 2, and the valve to be inspected is Valve 30. The
tubing for inspection is shown in Figure 3-35, and the isolation chamber to be inspected is the one
below the DIFF bath.
Figure 3-35 DIFF tubing for fluid discharge
3.6.10 Leakage from the Swab

First wipe off the liquid with tissue paper, and perform the following troubleshooting steps:
1. Check if Pump 1 is clogged.
Follow the steps specified in "3.6.3 Liquid Pump Clogging: Inspection and Troubleshooting" to
inspect and resolve any clogging of the liquid pump. After troubleshooting, run the CBC+DIFF
counting procedures to see if the problem has been resolved.
2. Check if Valve 19 is clogged.
Follow the steps specified in "3.6.2 Inspection and Troubleshooting of Valve Clogging" to inspect
and resolve any clogging of Valve 19. After the troubleshooting, run the CBC+DIFF counting
procedures to see if the problem has been resolved.
3. Check if there are any kinks or damage along the tubing.
Check if there are any kinks or damage along the tubing, as highlighted in red in Figure 3-36, and
replace the corresponding tubing if the problem persists. After the troubleshooting, run the
CBC+DIFF counting to see if the problem has been resolved.
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3 Hydraulics System
Figure 3-36 Swab tubing for fluid aspiration
4. Check if there is perforation at the bottom of the sample probe.
Set the small sample cup below the probe. When pulling out the tube above the probe (push the
tube end outward to pull it out; otherwise there will be crimping in the sampling tube connected to
the end of the probe), disassemble the probe, and connect it to the plastic syringe filled with the
diluent using a 1.0mm (i.d.) silicone tube. Manually push the syringe to see if any liquid flows
from the bottom end of the probe. If such a flow is detected, this indicates that perforation exists
at the bottom of the probe (the liquid normally flows from the sides. Be sure to collect the waste
when pushing the liquid to the correct location, as shown in Figure 3-37).
Figure 3-37 Check the liquid pushing of the sample probe
Restore the tubing to its original status (cut off the crimped part at the front end of the sampling
tube, and readjust the tubing for installation. Make sure to check if the sampling tubing gets into
the way of other assemblies whenever the sampling assembly moves up and down, or if it feels
too tight. If this is the case, continue to adjust the sampling tubing until it looks like it fits properly.)
5. Check for any wear and tear in the top aperture of the swab.
To disassemble, take off the swab's fixed jump ring. Pull out the swab's inflow and outflow tubes.
Measure the upper-section aperture with a caliper; an aperture with a diameter of more than
1.71mm indicates that the swab needs to be replaced.
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3 Hydraulics System
The inflow tube is the Thin 50 tube, connected to the bottom connector; the outflow tube is the
Thick 50 tube, connected to the top connector), then put on the swab (insert the probe into the
swab aperture) and tap the swab's fixed jump ring to complete the procedure.
3.6.11 Problems with Creating Positive Pressure

The troubleshooting steps are:
1. Check if the pressure chamber is broken: Disassemble the pressure chamber if necessary and
seal the openings with rubber tubes. Then place the pressure chamber into the water tank. Use a
syringe or other equipment to pressurize the pressure chamber. Any air bubbles detected
indicate that the pressure chamber needs to be replaced.
2. Check if there are any kinks or damage along the tubing to the pressure chamber. Any
replacements should be made using tubing of the proper length and type.
Figure 3-38 Tubing of the positive-pressure chamber
3. Check if the pump is working; if not, replace the pump.

4. Check if the positive-pressure sensor and the corresponding electrical circuit are in normal
working order. First replace a reagent testing panel and reconnect its tubing, then check if the
positive pressure is normal in the status interface; if it is normal after the replacement, this means
that the positive-pressure sensor and the corresponding electrical circuit are causing a problem
with building positive pressure (After the reagent testing panel is replaced, note that the tubing
needs to be connected correctly. First keep a record of where it was by marking it before
disassembling the tubing).
5. Check the valve directly connected to the tubing of the positive-pressure chamber to see 1)
whether the on-off switch is normally; and 2) whether there is any clogging.
6. After troubleshooting, complete the steps shown in the troubleshooting wizard.
3.6.12 Problems in Creating the Negative Pressure

The troubleshooting steps are:
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3 Hydraulics System
1. Check if the negative-pressure chamber is broken: Disassemble the negative-pressure chamber

if necessary and seal the openings with rubber tubes. Then put the negative-pressure chamber
into the water tank. Use a syringe or other equipment to pressurize the negative-pressure
chamber. Any air bubbles that occur indicate that the negative-pressure chamber needs to be
replaced.
2. Check if there are any kinks or damage along the tubing connected to the negative-pressure
chamber. Any replacements should be made using tubing of the proper length and type.
Figure 3-39 Tubing of the negative-pressure chamber
3. Check if Liquid Pump 2 is working; if not, replace the pump.

4. Check if the negative pressure sensor and the corresponding electrical circuit are in normal
working order. First replace a reagent testing panel and reconnect its tubing; then check if the
negative pressure is normal in status interface. If it is normal after the replacement, this means
that the negative-pressure sensor and the corresponding electrical circuit have caused a
problem with building negative pressure (After the reagent testing panel is replaced, note that the
tubing needs to be connected correctly. First keep a record of where it was by marking it before
disassembling the tubing).
5. Check the valve directly connected to tubing of the negative-pressure chamber to see 1) whether
the on-off switch is normally; and 2) whether there is any clogging.
6. After troubleshooting, complete the steps shown in the troubleshooting wizard.
3.6.13 Clogging of the Air Filter

Replace the air filter.
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3.6.14 No Scattergram
Troubleshooting steps are as follows:
1. Check if the two tubes are connected correctly in pinch valve LV22.First confirm that they are
correctly positioned; then determine whether both tubes are tucked into the bottom of the round
tank (as shown in Figure 3-40).
Figure 3-40 Tubing connection of pinch valve
2. If the problem persists after the inspection, please check if the optical system is working properly
(see the section about common problems of the optical system and their solutions)
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4 Hardware System
4.1 Hardware System

A computer must be physically connected to the Analyzer via a network cable. The hardware system
of the device consists of the main control panel, driver panel, rear panel, autoloader panel, reagent
testing panel, laser driver panel, optical preamplifier panel, and front panel. The block diagram is
shown below:
Main control Driver panel

panel
Autoloader Laser driver

panel panel
Rear Panel Optical
Reagent testing
panel preamplifier
panel
Other wiring devices (motors, Front panel (LED,

valves, sensors , etc) buzzer)
Hardware maintenance principles apply to all the panels. All the panels have passed hardware tests
before leaving the factory, and any hardware problem found later may be mainly caused by power
problems. Thus, hardware maintenance primarily involves the power supply. Hardware problems
and maintenance methods for each panel are described in the subsequent sections.
Apart from the main control panel, driver panel, rear panel, and the driver panel for the autoloader,
the other panels generally do not require maintenance. Immediately replace any small panels in
which issues have been identified.
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4.2 Main Control Panel

The main control panel is the core panel of the DH hematology analyzer; the device cannot work
properly with a problematic main control panel. This section introduces the main control panel from
the perspectives of composition, problem identification, and maintenance.
4.2.1 Composition of the Main Control Panel
External analog
External digital interface
interface
Power supply, I/O, etc.

External interface for network and others
Upgrade interface, I/O, etc.
Analog
impedance
Impedance input
input
Analog DIFF
AD module DIFF input
Collection channel
ARM unit Communication FPGA unit Monitoring variable input

Analog
hardware
monitoring
channel
Power supply, Power-on /Reset
Analog power Power supply

supply
Power supply
Co
ARM power HGB drive

ntr
supply
ol
Power supply
sig
na
l
FPGA Power Analog control CC source

CC source control
supply signal
Scorching control
Zap
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4 Hardware System
The block diagram above gives a brief overview of the composition of main control panel and the
function of each module. Further description can be found below based on the pictures for each
panel.
Figure 4-1 Main control panel
It is clear that main control panel comprises s digital section and an analog section. The former is on
the left half of the panel, including but not limited to the ARM unit, FPGA unit, peripheral interface,
troubleshooting interface, and power supply. The latter is on the right half of the panel, including but
not limited to the impedance channel, DIFF channel, hardware monitoring channel, peripheral
interface, and power supply. In the middle of the panel are the AD and interface chips; the former is
designed for analog-digital conversion, while the latter is used as a control for switching between
analog functions.
4.2.2 Peripheral Interface of the Main Control Panel

The interfaces designated for the main control panel are U1, U2, J5 and J6. U1 is the peripheral
digital interface of the main control panel, while U2 is its peripheral analog interface. They are
respectively connected to J36 and J7 on the rear panel. Before plugging in, please ensure a reliable
connection between U1 and J36 as well as between U2 and J7. J5 is the socket for the lead wire of
the RBC bath, while J6 is the socket for the lead wire of the WBC bath.
The troubleshooting interfaces designated for the main control panel are serial interface J7 (RS232
signals), FPGA JTAG interface J3, FPGA AS interface J4, and SD interface for programming before
shipping. Use of these troubleshooting interfaces is restricted to developers only.
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4.2.3 Power Input and Indicator Lights on the Main Control Panel
Digital power input and indicator lights

The 5V digital power input can be tested through the testing point TP137 (using the multimeter for
the testing, where the red test pen should be connected to the TP137 electrode while the black test
pen should be connected to the metal enclosure) The 5V power input is accompanied by the fuse
F45. Normally the fuse will not be blown since the power supply of the device is equipped with surge
protection. If a short circuit occurs under the input of 5V of power, the power supply will fail to work
properly and a buzzing sound can be heard.
The main control panel has no 5V digital power indicator light, but has some other digital power
indicator lights. See the following table for details:
Position Code of Meaning Normal Status

Indicator Light
D2 FPGA 3.3V power supply On
D5 FPGA 1.2V power supply Off1
D43 TPS65910 3.3V working status2 On
D47 Peripheral device 3.3V power supply On

Note:
1- 1.2V fails to turn on the LED. The corresponding testing points can be tested using a multimeter if
necessary. For details, see Digital Testing Points in the section of 4.2.4 Testing Points on the Main
Control Panel.
2- D43 usually indicates the working status of the ARM power management chip, TPS65910A31A1, but
this indicator light only indicates the working status of one LDO signal channel in TPS65910; the
multimeter can be used to test the corresponding testing points for other LDO and DC-DC channels. For
details, see Digital Testing Points in the section of 4.2.4 Testing Points on the Main Control
Panel.
Analog power input and indicator lights

The analog power input of +12V and -12V on the main control panel can be tested for actual voltage
values via the testing points TP70 and TP72. The +12V power input is accompanied by the fuse F2,
while -12V is accompanied by the fuse F1. Like the 5V digital power input, +12V and -12V derive
power from the device's power supply; if both are subject to overload, they will receive surge
protection from the power source and the corresponding indicator lights will not be activated.
The following table lists the analog power indicator lights:

Indicator Light
D17 -12V power indicator light On
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Indicator Light
D20 +12V power indicator light On
D21 -5V power indicator light On
D22 +5V power indicator light On

+5V and -5V originate from the linear stabilizer. The corresponding lights will not be activated by the
+5V or -5V overload, and the linear stabilizer will be very hot. If either the +5V or the -5V power
indicator light is not on, immediately power off the device and troubleshoot the corresponding
electrical circuits. We recommend replacing the main control panel.
Other indicator lights on the main control panel

The ARM and FPGA units are each assigned a single indicator light to show their working status on
the main control panel. In addition, a USB0 power indicator light is designated for the ARM unit.
The following table lists the indicator lights:

Indicator Light
D46 It indicates the ARM working status and flashes Flashing

after proper system loading.
D48 It indicates the FPGA working status and flashes Flashing

after proper system loading.
D1 USB0 power indicator light; it is on when USB0 is On/Flashing

properly initialized, which implies that ARM system
is working properly
Indicator lights are very useful in practice; hardware problems can be identified by the status of the
indicator lights. For details, see the section on the identification of main control panel problems.
4.2.4 Testing Points on the Main Control Panel

There are two types of testing points on the main control panel: Digital and analog.
Digital testing points

The digital testing points on the main control panel listed here are commonly used ones, in particular
for power supply and key signals:
Position Code of Description

Testing Points
TP137 the testing point for 5V digital input, with the expected voltage of 5V
TP138 TPS65910 VRTC output, with the expected voltage of 1.8V
TP149 DDR3 reference voltage, with the expected value of 0.75V
TP146 TPS65910 VDIG1 output, with the expected value of 1.8V
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Testing Points
TP147 TPS65910 VDIG2 output, with the expected value of 1.8V
TP144 TPS65910 VAUX33 output, with the expected value of 3.3V
TP145 TPS65910 VMMC output, with the expected value of 3.3V
TP140 TPS65910 VDAC output, with the expected value of 1.8V
TP141 TPS65910 VPLL output, with the expected value of 1.8V
TP8 the testing point for FPGA 3.3V power supply, with the expected voltage of
3.3V
2.5V
1.2V
TP9 the testing point for FPGA analog power supply, with the expected voltage
of 2.5V
TP10 the testing point for FPGA PLL power supply, with the expected voltage of
1.2V
1.8V
TP85 DIFF low-angle AD clock,4MHz clock signal
TP90 DIFF medium-angle AD clock, 4MHz clock signal
TP87 DIFF high-angle AD clock, 4MHz clock signal
TP126 WBC-channel AD clock, 1MHz clock signal
TP127 PLT-channel AD clock, 1MHz clock signal
TP123 RBC-channel AD clock, 1MHz clock signal
Analog testing points

The following table lists the analog testing points:

Testing Points
TP71 AVCC_+5V testing point, with the expected voltage of 5V
TP125 Analog ground point
TP70 AVCC_+12V testing point, with the expected voltage of +12V
TP86 VCCA2_3V3, with the expected voltage of 3.3V
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Testing Points
TP72 AVCC_-12V testing point, with the expected voltage of -12V
TP73 AVCC_-5V testing point, with the expected voltage of -5V
TP97 VBURN_CTL testing point, the control switch for the zap source; 0 for on
and 1 for off
TP93 HGB_LED_CTL testing point, HGB light-driven control switch; 0 for on and
1 for off
TP133 WBC channel first-level OPAMP output testing point
TP112 WBC channel fourth-level OPAMP output testing point
TP114 WBC channel sixth-level OPAMP output testing point
TP116 WBC channel seventh-level OPAMP output testing point
TP115 WBC channel eighth-level OPAMP output testing point
TP104 RBC/PLT channel first-level OPAMP output testing point
TP105 RBC/PLT channel fourth-level OPAMP output testing point
TP106 RBC channel sixth-level OPAMP output testing point
TP107 RBC channel seventh-level OPAMP output testing point
TP108 RBC channel eighth-level OPAMP output testing point
TP109 PLT channel seventh-level OPAMP output testing point
TP110 PLT channel eighth-level OPAMP output testing point
TP111 PLT channel ninth-level OPAMP output testing point
TP102 SELECT_WBC_CTL testing point, the switch control signal for WBC bath
zap and CC source; 0 for zap and 1 for CC source
TP99 SELECT_RBC_CTL testing point, the switch control signal for RBC bath
zap and CC source; 0 for zap and 1 for CC source
TP119 VCONST_MON_AD testing point, 1/41 of CC source voltage
TP100 RH_MON testing point, indirectly reflecting RBC-bath aperture voltage
TP101 WH_MON testing point, indirectly reflecting WBC-bath aperture voltage
TP98 VCONST_CTL testing point, the switch control signal for CC source; 0 for
on and 1 for off
TP96 The oscillator clock output for the voltage doubling circuit, 363KHz clock
signal
TP76 the testing point for DIFF low-angle signal input
TP74 the testing point for DIFF low-angle second-level OPAMP output
TP75 the testing point for DIFF low-angle fourth-level OPAMP output
TP77 the testing point for DIFF medium-angle signal input
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Testing Points
TP78 the testing point for DIFF medium-angle second-level OPAMP output
TP79 the testing point for DIFF medium-angle fourth-level OPAMP output
TP80 the testing point for DIFF high-angle signal input
TP81 the testing point for DIFF high-angle second-level OPAMP output
TP82 the testing point for DIFF high-angle fourth-level OPAMP output
TP83 DIFF low-angle AD analog input
TP91 DIFF medium-angle AD analog input
TP88 DIFF high-angle AD analog input
TP94 First-level OPAMP output of HGB channel
TP95 Second-level OPAMP output of HGB channel
TP118 LASER_MON_AD testing point, reflecting the laser current
TP121 AVCC_+12VMON_AD testing point, reflecting the voltage of AVCC_+12V

power source
TP122 AVCC_-12VMON_AD testing point, reflecting the voltage of AVCC_+12V

power source
TP124 AD Analog input of RBC channel
TP128 AD Analog input of PLT channel
TP131 AD Analog input of WBC channel
4.2.5 Identification of Main Control Panel Problems

Problems with the main control panel can be categorized as power problems, connection problems,
and functional problems. Power problems can be identified using the status of power indicator lights,
and connection problems can identified by way of direct observation; however, functional problems
are a little tricky, and can be indirectly shown using other indicators.
Power Problems of the Main Control Panel and their Indicators

The power supply of the main control panel is clearly divided between digital and analog. Of these,
 A digital power input of 5V is realized by the left connector U1 on the main control panel, as
shown in Figure 4-2.
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4 Hardware System
Figure 4-2 Digital power input and testing point
 An analog power input of +12V and -12V is enabled by the right connector U2 on the main
control panel, as shown in Figure 4-3.
Figure 4-3 Analog power input
Both digital and analog power inputs on the main control panel derive from the power input of the
device. If either of the power inputs on the main control panel receives a surge from a grounded or
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any other input power source, the device’s power supply will activate its automatic protection
mechanism and produce a buzzing sound.
The digital power input does not have any indicator light, whereas the analog power input does, as
shown in Figure 4-4.
Figure 4-4 Analog power indicator light on the main control panel
Other digital power indicator lights are shown in Figure 4-5.

Figure 4-5 Digital power indicator light on the main control panel
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For the normal status of power indicator lights shown in the figures above, please refer to the
descriptions of the power supply and indicator lights of the main control panel in "4.2.1 Composition
of the Main Control Panel". If the power indicator light is in a status different from normal, this means
that the power supply is not working properly.
U74 deserves further description as follows. U74 is the ARM power management chip in the digital
section of the main control panel. The working status of this chip determines the working status of
the main control panel. As shown in Figure 4-6, D43 turned on indicates the proper working of U74.
Furthermore, the voltage of each testing point when U74 is working properly should be consistent
with the screen printing on the enclosure.
Figure 4-6 ARM power management chip in the main control panel
Connection Problems with the Main Control Panel and their Indicators
Connection problems with the main control panel mainly involve the reliability of connections
between the main control panel and the rear panel, as well as the counting-bath lead wire and the of
main control panel's sockets.
 The reliability of connections between main control panel and the rear panel can be ensured
using the PCB handle and screw.
The correct plugging method is shown in Figure 4-7 and Figure 4-8.
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4 Hardware System
Figure 4-7 PCB handle and screw need to be set in position on the panel
Figure 4-8 The reliability of the connection between the main control panel and the rear panel
needs to be guaranteed
Avoid the connection mistakes as shown in Figure 4-9 and Figure 4-10.
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Figure 4-9 Improper installation
Figure 4-10 Improper installation
 When connecting the lead wire of the counting baths to the main control panel, pay attention to
the order in which the lead wires are plugged in. Normally the RBC-bath lead wire needs to be
plugged into J5, while the WBC-bath lead wire must be plugged into J6.
The illustration shows the sockets on the main control panel, and the lead wires of each counting
bath also have relevant markings. See Figure 4-11 and Figure 4-12, respectively.
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Figure 4-11 Positions for plugging the counting-bath lead wires into the main control panel
Figure 4-12 Counting-bath lead wires
If the network cable in the device packaging is only used for the physical connection between the
device and the PC, the it needs to be a straight-through network cable (i.e. A-wire); if the device is
connected to a network, make sure that the switch has a self-adapted network-port line sequence (and
AUTO MDI/MDIX function) enabled. The straight-through network cable is applicable if the switch is
capable of self-adaption for the network-port line sequence. If no such function is available, use the
crossover network cable (i.e. B-wire).Most current switches are equipped with self-adapting
network-port line sequences. The network cable included in the shipping package is normally the
A-wire.
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Functional Problems with the Main Control Panel and their Indicators
Functional Problems with the main control panel mainly refer to situations wherein the main control
panel fails to implement the counting function. Specifically, the digital OS of the main control panel
fails to boot, FPGA program loading fails, and counting abnormalities are identified. These possible
occurrences are described in detail below.
 ARM OS on the main control panel fails to boot
The ARM on the main control panel boots from NAND using its default factory setting, so the
booting media selection switch S3 should be turned to NAND (i.e. OFF state for DIP switches),
as shown in Figure 4-13.
Figure 4-13 ARM booting and running
When the ARM OS is booted properly, the working-status indicator light D46 will flash on the
main control panel. If D46 is not lit up, it is very likely that the ARM OS has failed to boot.
 Loading problems with the FPGA program
The FPGA program is loaded from the serial port FLASH.A successful loading of the FPGA will
cause the indicator light D48 to flash. If D48 is not lit up, it means that FPGA program has not
loaded. The location of D48 is shown in Figure 4-14.
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Figure 4-14 Indicator of FPGA working status
 Counting abnormalities
There are many causes that lead to counting abnormalities, and they can include problems with
main control panel problem. Counting abnormalities caused by main control panel problems
usually involve counting results of zero or overly high counting results on multiple occasions. A
counting result of zero usually results from a failure to apply the CC source to the counting bath,
while an overly high counting result is usually caused by too much noise in the analog-channel
circuit.
4.2.6 Maintenance of the Main Control Panel

The maintenance of the main control panel mostly deals with problems not related to connections. In
theory, only designated maintenance professionals are allowed to perform the maintenance steps for
the main control panel. Please replace the main control panel if any non-connection problems occur.
The instructions above only apply to designated maintenance professionals.
Locating Main Control Panel Problems

Successful troubleshooting is a precondition of maintenance. Main control panel problems mostly
involve the power supply. As for functional problems, there is no real value in maintenance and the
main control panel should therefore be replaced at your earliest convenience.
There are four steps in locating power-related problems:
 Step 1. Look. Power on the panel and pay attention to the power indicator lights. If any
abnormality is spotted, then there is something wrong with the loading of the indicator light’s
corresponding power source. Light abnormality here refers to situations in which a light is off or
dimmer than normal.
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 Step 2. Smell. Power problems may burn out some components; an acrid smell can suggest a
burnout. Do not power on in this case.
 Step 3. Touch. Power on and touch the corresponding component on the main control panel by
hand. If the temperature feels abnormal, the component could be broken.
 Step 4. Test. The first three steps can be used to identify power overload problems on the main
control panel. Step 4 is designed for determining what has malfunctioned. Step 4. tests the first
three steps can be used to identify power overload problems on the main control panel. Step 4 is
designed for determining what has malfunctioned. Testing can also identify the problem of circuit
breakage. This step is also part of maintenance. A multimeter is used to test for power problems.
The following section lists common problems found in panel maintenance and their relevant
indicators.
Common Problems Found in Main Control Panel Maintenance and their Indicators
See the following table for details:
Problem Description Problem Indicators Solution
The panel is not set right during Insert the panel into the
replacement, resulting in the loss correct position and tighten
of some power input or a the screws.
disconnection of the
communication cable to the rear
panel. This can lead to problems
with network disconnection and
power-on initialization.
Analogy power input of -12V is The power indicator light D17 is not This issue is mostly
not loaded, and the device power activated. caused by the fact that the
supply is switched into auto analog power input of -12V
protection mode when powered has shorted out. Another
on. possible reason is the
breakdown of the polar
capacitors (mostly
tantalum capacitors here).
Due to the abundance of
-12V networks, users are
recommended to replace
the motherboard. The
damaged components can
be located by PCB for
replacement by qualified
maintenance personnel.
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Analogy power input of +12V is The power indicator light D20 is not This issue is mostly
not loaded, and the device power activated. caused by the fact that the
supply is switched into auto analog power input of
protection mode when powered +12V has shorted out.
on. Another possible reason is
the breakdown of the polar
capacitors (mostly
+12V networks, users are
Analogy power input of -5V is not The power indicator light D21 is not This issue is mostly
loaded lit up or looks dim, and the power caused by the fact that the
chip U28 feels very hot. analog power input of -5V
has shorted out. Another
possible reason is the
capacitors (mostly
-5V networks, users are
Analogy power input of +5V is not The power indicator light D22 is not This issue is mostly
loaded lit up or looks dim, and the power caused by the fact that the
chip U30 feels very hot. analog power input of +5V
has shorted out. Another
possible reason is the
capacitors (mostly
+5V networks, users are
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4 Hardware System
The ARM system on the main The powered-on device fails to do The power management
control panel is not running self-test; the ARM status indicator chip U74 may be
light D46 is not flashing and the damaged; it is
indicator light for ARM power recommended that a
management chip D43 is lit up. qualified maintenance
person replace U74.
The ARM system on the main The powered-on device fails to do Some circuit in the power
control panel is not running self-test; the ARM status indicator management chip U74
light D46 is not flashing and the may have shorted out.
indicator light for ARM power Qualified maintenance
management chip D43 is on. personnel can use a
multimeter to test the
power output testing points
throughout U74 to locate
the problematic circuit.
Start from the capacitor
and move along until
locating the short circuit.
The ARM system on the main The powered-on device fails to do The boot media for the
control panel is not running self-test; the ARM status indicator main control panel, NAND
light D46 is not flashing and the Flash U79, may be
indicator light for ARM power damaged; replace the
management chip D43 is on. A main control panel as soon
multimeter is used to test the as possible.
power management chip only to
find that the power output is normal
throughout the chip and the device
lifetime can last for more than three
years.
The ARM system on the main The powered-on device fails to do The ARM of the main
control panel is not running self-test; the ARM status indicator control panel is damaged;
light D46 is not flashing and the replace the main control
indicator light for ARM power panel immediately.
management chip D43 is on. A
multimeter is used to test the
power management chip only to
find that the power output is normal
throughout the chip and the ARM
chip U62 feels hot.
The FPGA program fails to load The FPGA running status indicator The FPGA on the main
light, D48, is not flashing, the control panel is damaged;
FPGA 3.3V power indicator light replace the main control
D47 is in normal ON status, and so panel immediately.
is the FPGA 2.5V power indicator
light D3. The FPGA 1.8V power
indicator light, D4, is in normal ON
status, the FPGA1.2V power
indicator light is off, but TP7
voltage is normal while TP11
voltage is low as measured with a
multimeter.
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4 Hardware System
The FPGA program fails to load The FPGA running status indicator The FGPA 3.3V power
light, D48, is not flashing, and the circuit is problematic and
FPGA 3.3V power indicator light the issue can be located by
D47 is not lit up. multimeter testing.
D5 is not lit up. Test the FPGA 1.2V multimeter testing.
power with a multimeter, and the
voltage tested at testing point TP7
is not 1.2V.
Liquid has been spilled onto the The main control panel is eroded. Replace the main control
main control panel. panel
Panel maintenance is time-consuming. In principle, panel maintenance is not performed on-site. If a

panel problem is confirmed on-site, please replace the panel.
4.3 Driver Panel

The driver panel is the implementation unit of the device, and is designed for driving the pump, valve,
and motor, and collecting the relevant signals. This section introduces the driver panel from three
perspectives: Overview, problem identification, and maintenance.
4.3.1 Overview of the Driver Panel

The driver panel needs to accommodate for many input and output signals, so the current platform
architecture of the driver panel is MPU + FPGA. MPU is designed for communication, while FPGA is
designed for signal input and output.
Divided into several functional modules, the main schematic diagram of the driver panel is shown as
follows:
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4 Hardware System
Temperature control
Analog input ARM module
Communication interface
Communication interface
Power supply Power supply Power supply and
input reset signal
and reset
External interface
Drive signal Valve drive Control signal
Drive signal Motor drive Control signal
FPGA module
Drive signal Pump drive Control signal
Digital signal switch input
The schematic diagram shows the functional modules of the driver panel. Figure 4-15 is the actual
picture taken of the driver panel and indicates the function of each module.
Figure 4-15 Picture taken of the driver panel
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4 Hardware System
The peripheral ports of the driver panel are equipped with two 120-pin European sockets; the left port
U5 is the driver outlet, while the right port U4 is the signal and power inlet. The tuning port is to be
used by qualified tuning personnel only.
4.3.2 The Indicator Lights for the Driver Panel

The indicator lights for the driver panel include the power indicator lights, running status indicator
lights, and I/O signal indicator lights.
Position Code Meaning Normal Status

of Indicator
Light
D49 3.3V power indicator light for On

drive-panel ARM
D50 2.5V power indicator light for On

drive-panel FPGA
D51 1.2V power indicator light for Off1

drive-panel FPGA
D4 a flashing green light indicates that Flashing

the program is running properly
D5 a flashing red light indicates a Off

problem with the drive-panel system
D27 reserved for FPGA; not in use Off
D28 FPGA program loading and Flashing

configuration of the motor drive chip
are complete.
D6 locked-rotor indicator light for the On for locked-rotor motor; Off for motor
vertical motor (Motor 8) rundown.
D7 STEP indicator light for the vertical On for motor rundown

motor (Motor 8)
diluent syringe (Motor 4) rundown.
D9 STEP indicator light for the diluent On for motor rundown

syringe (Motor 4)
horizontal motor (Motor 7) rundown.
D11 STEP indicator light for the horizontal On for motor rundown
motor (Motor 7)
D12 locked-rotor indicator light for the lyse On for locked-rotor motor; Off for motor
syringe (Motor 3) rundown.
D13 STEP indicator light for the lyse On for motor rundown
syringe (Motor 3)
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4 Hardware System

of Indicator
Light
sample syringe2 (Motor 1) rundown.
D15 STEP indicator light for the sample On for motor rundown
syringe (Motor 1)
sheath flow syringe (Motor 5) rundown.
D17 STEP light for the sheath flow syringe On for motor rundown
(Motor 5)
reserved syringe (Motor 6) rundown.
D19 STEP indicator light for the reserved On for motor rundown
syringe (Motor 6)
sample injection syringe (Motor 2) rundown.
D21 STEP indicator light for the sample On for motor rundown
injection syringe (Motor 2)
D29 input status indicator light for the Off when blocked by the optocoupler; On
optocoupler in the initial position of when not blocked by the optocoupler
the sample syringe
the sample injection syringe
the lyse syringe
the diluent syringe
the sheath flow syringe
the horizontal motor
optocoupler in the horizontal position when not blocked by the optocoupler
of WBC bath
optocoupler in the horizontal position when not blocked by the optocoupler
of DIFF bath
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4 Hardware System

of Indicator
Light
optocoupler in the horizontal open when not blocked by the optocoupler
sampling position
the vertical motor
reserved optocoupler when not blocked by the optocoupler
Note:
1- 1.2V fails to activate the LED, so the light is OFF.
2- The sample syringe and sample injection syringe currently share Motor 1.
4.3.3 Main Testing Points of the Driver Panel

Position Code of Testing Points Description
TP43 testing point of 5V digital power input, with the

expected voltage of 5V
TP44 testing point of 3.3V digital power input, with the

expected voltage of 3.3V
TP23, TP24, TP25, TP26, TP27, TP28 ground points for digital testing
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4 Hardware System
Position Code of Testing Points Description
TP20, TP21, TP22, TP29, TP30, TP31 ground points for power testing
TP3 CANH testing point
TP4 CANL testing point
TP2 testing point for 12MHz clock signal
TP1 output testing point for drive-panel ARM reset
TP45 the testing point for FPGA 2.5V power supply, with the
TP19 the testing point for power network VCCA, with the
TP18 the testing point for power network VCCD_PLL, with

the expected voltage of 1.2V
TP46 the testing point for FPGA 1.2V power supply, with the
TP15 testing point for 50MHz clock signal
4.3.4 Identification of Driver Panel Problems

Problems with the driver panel can include power problems, connection problems, and functional
problems, the most prevalent of which are power problems.
Power Problems with the Driver panel

There are three channels of power input in the driver panel: 5V digital input, 12V power input, and
24V power input.
 5V digital input is the power input for all the chips working on the driver panel (see the actual
picture Figure 4-16)
 12V power input serves the pumps and valves (see the actual pictures Figure 4-17)
 24V power input drives the motors (see the actual picture Figure 4-17)
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4 Hardware System
Figure 4-16 5V digital input of the driver panel
Figure 4-17 Power input of the driver panel
If either channel of power input has shorted out, the power source will activate its automatic surge
protection system and emit a buzzing sound.
The chips working on the driver panel also need power inputs of 3.3V, 2.5V and 1.2V.The actual
pictures taken of the power-supply circuits are Figure 4-18, Figure 4-19 and Figure 4-20,
respectively.
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4 Hardware System
Figure 4-18 3.3V power supply of the driver panel
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4 Hardware System
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4 Hardware System
The normal status of each power indicator light after the driver panel is powered on is shown in the
section "4.3.2 The Indicator Lights for the Driver Panel". If the actual status of any indicator light is
different, it is easy to locate the problem in a channel of power input.
Connection Problems with the Driver panel

The peripheral ports of the driver panel are U4 and U5, and a connection problem refers to an
unreliable connection between the driver panel and the rear panel. A probable cause is that the
driver panel was disassembled and standard connection procedures were not followed when it was
reattached. This is similar to connection problems with the main control panel, which call for special
on-site care.
Functional Problems with the Driver panel

A functional problem with the driver panel means that the driver panel is not functioning properly.
MPU or FPGA problems can be identified by the running status indicator lights, while driver unit
problems can only be indirectly identified by checking the peripheral components.
 MPU program loading fails
D4 in Figure 4-21 is not flashing.
Figure 4-21 MPU indicator light on the driver panel
 Driver panel system problems

In Figure 4-21, if the indicator light D5 is flashing, it indicates a drive-panel system problem and
possibly a MPU-FPGA communication problem, CAN bus problem, device power-on initialization
problem, etc.
 The FPGA program fails to load
After the FPGA is properly loaded, the FPGA running status indicator light, D28 in Figure 4-22,
will flash. It not flashing indicates a FPGA problem.
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4 Hardware System
Figure 4-22 Indicator light of FPGA running status
4.3.5 Maintenance of the Driver Panel

No maintenance of panels can be performed on-site. Panel maintenance only applies to authorized
maintenance professionals.
Prior to panel maintenance, please locate the panel problem by following the steps in 4.2.6
Maintenance of the Main Control Panel.
The following table lists common driver panel problems.
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4 Hardware System
Problem Problem Indicators Solution

Description
The driver 3.3V power indicator light Check the availability of 5V input and the output of the
panel is not D49 is off. 3.3V power chip.
working.  If the 5V input is unavailable, pull out the panel to
test if the 5V input has shorted out.
If it has, replace the panel; if not, the problem is most
likely caused by an unreliable connection.
 If it is confirmed that the 3.3V power chip has no
output, power off the panel and test if the 3.3V
power network has shorted out.
If it has, replace the panel right away; if not, it is very
likely that the power chip U22 is broken, and that only
the U22 needs to be replaced. When welding the
U22, Pin 6 and 7 need to be short-circuited as shown
in the figure:
The driver The 5V input is working, Test if the 2.5V power network has shorted out. If it has,
panel is not but the 2.5V power replace the driver panel right away; if not, replace the
working. indicator light D50 is not power chip U20.
on.
The driver The 5V input is working, Test if the 1.2V power network has shorted out. If it has,
panel is not but the 1.2V power replace the driver panel as soon as possible; if not,
working. indicator light is not on replace the power chip U21.
and the voltage of the
testing point TP46 is not
1.2V.
The driver All the power sources are Replace the driver panel.
panel is not working properly, but the
working. MPU running status
indicator light D4 is not
flashing.
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4 Hardware System
Problem Problem Indicators Solution

Description
The driver All the power sources are Replace the driver panel.
panel is not working properly, but the
working. FPGA running status
indicator light D28 is not
flashing.
The driver The hydraulic tubes have Some valves' drivers may have malfunctioned; it is
panel is not collapsed. suggested that the driver panel be replaced as soon as
working possible. If the problem persists, check the driver path for
properly. valves, since this problem is usually caused by wiring
issues or a broken valve.
The driver The pump is not working. First confirm the reliability of the connection between the
panel is not driver panel and the rear panel. If the connection is
working reliable, then the pump may have a problematic driver; it
properly. is suggested that the driver panel be replaced right away.
If the problem persists, check the driver path for pumps,
since this problem is mostly caused by wiring issues or a
broken pump.
The driver The motor is not working. First confirm the reliability of the connection between the
panel is not driver panel and the rear panel. If the connection is
working reliable, then the motor may have a problematic driver; it
properly. is suggested that the driver panel be replaced right away.
If the problem persists, check the driver path for motors,
since this problem is mostly caused by wiring issues.
The driver The driver panel has no Some heating systems may have a problematic driver; it
panel is not heating. is suggested that the driver panel be replaced right away.
working If the problem persists, check the driver path for heating,
properly. since this problem is mostly caused by wiring issues.
4.4 Rear Panel

The rear panel needs no maintenance in principle and must not be disassembled as long as it has
not short circuited. Figure 4-23 is an actual picture taken of the rear panel and shows the function of
each port.
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4 Hardware System
Figure 4-23 Actual pictures taken of each rear-panel port
4.5 Autoloader
Primary functions of the autoloader panel:
 Responsible for the loading, transfer, and unloading of each row of test tubes.
 Test the barcode of each test tube.
 Pick up and shake the test tube which meets the test criteria.
The functions of ports on the autoloader panel are shown in Figure 4-24.
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4 Hardware System
Figure 4-24 Picture of the autoloader panel

Special port
Reset Power supply Motor drive port
for software input port
key
development
Position Opticoupler
Barcode detection signal detection signal Motor
scanner port input port input port drive port
4.6 Reagent Testing Panel

The main function of the reagent testing panel is to check the presence of the reagent. The functions
of ports on the reagent testing panel are as shown in Figure 4-25 and Figure 4-26.
Figure 4-25 Picture of the top of the reagent testing panel
Optocoupler detector
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4 Hardware System
Figure 4-26 Picture of the bottom of the reagent testing panel
Sheath flow
pressure sensor
signal input port
Positive
Pressure sensor pressure sensor
signal output port
Opticoupler Negative
detection signal pressure sensor
output port
4.7 Indicator Panel

The main function of the indicator panel is to show the working status of the machine. Its functions of
the ports are as shown in Figure 4-27 and Figure 4-28.
Figure 4-27 Picture of the Top of the Indicator Panel
Autoloader Power supply and
key port control signal port
Sample collection
key port
Figure 4-28 Picture of the Bottom of the Indicator Panel
Machine-on indicator light
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4 Hardware System
4.8 Key Panel

The key panel mainly provides the user with keyboard commands for the starting of the machine.
Their pictures are shown in Figure 4-29 and Figure 4-30.
Figure 4-29 Picture of the Top of the Key Panel
Reserved key Autoloader key
Figure 4-30 Picture of the Bottom of the Key Panel

Reserved Autoloader
key port key port
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5 Optical System
5 Optical System
5.1 Integral Replacement of the Optical Assembly

Purpose
The DIFF bath assembly can be disassembled and replaced by following the procedures specified in
this section; however, in each case, "Preliminary Steps" needs to be taken before you begin.
Tools/Spare Parts
 #2 (Ph2) Phillips screwdriver
 Medical syringe attached to a silicone tube
 Optical components that have passed tuning tests
Preliminary Steps
1. Turn off the power switch on the left side of the device.
2. Pull out the power cord plug from the rear panel of the device.
3. Open the right-side door and disassemble the top panel cover.
Disassembly
1. Use a #2 (Ph2) Phillips screwdriver to disassemble the four M3×8 cross-recessed pan-head
combination screws which affix the cover panel of the optical system, and carefully detach it.
1- M3×8 cross-recessed pan-head 2-cover panel of the

combination screw (×4) optical system
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5 Optical System
2. Pull out the tubing. First pull out Tube P1 of Connector 2 in Figure 5-1 and connect the medical
syringe with the silicone tube to Connector 2. Pull out Tube P6 from the L-type connector at the
top end of Connector 1. Drain the water from the sheath flow device with a syringe and pull out
Tube P21 and P4c from the Connector 3 and 4 at the bottom of the flow chamber.
Figure 5-1 Pulling out the tubing
1- Tube P6 2- Tube P1
3- Tube P21 3- Tube P4c

combination screws which affix the optical assembly, and take off the four large D3 gaskets.
Carefully push upwards and slowly detach the optical assembly.
Figure 5-2 Disassembling the optical assembly
1- M3×8 cross-recessed pan-head 2- Large D3 gasket

combination screw (×4)
4. Pull out the wires from the J66 and J4 ports on the rear panel; then pull out the heating wire
inside the optical system box, the temperature sensor wire, and the temperature switch wire. To
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5 Optical System
take the optical assembly apart, slowly hold it up and remove the flow chamber from the opening
of the affixed optical panel.
Installation
Follow the corresponding disassembly steps in reverse order.
 Put on antistatic gloves and an electrostatic-shielding ring while operating to avoid damaging the
LED.
 All the wirings need to be set up according to the original positions of the machine components
to prevent them from being crushed or otherwise damaged.
 During transportation and installation, the flow chamber exposed at the bottom of the optical
system needs to be protected from being crushed or pressed. When pulling out the tubes, hold
the flow chamber by hand to prevent it from being dislocated by external force.
5.2 Replacing the Optical Preamplifier Panel

Purpose
The optical preamp panel can be disassembled and replaced by following the procedures specified
in this section; however, in each case, "Preliminary Steps" needs to be taken before you begin.
Tools/Spare Parts
 New optical preamplifier panel
Preliminary Steps
4. Take down the cover panel of the optical system box.
Disassembly
1. Pull out the three wires from the optical preamp panel.
combination screws which affix the optical preamp panel, and carefully detach the cover panel of
the optical system box.
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5 Optical System
Figure 5-3 Taking down the cover panel of the optical system box.
1- M3×8 cross-recessed pan-head 2-Optical preamplifier panel

3-Pre-optical fixation panel
Installation
LED.
5.3 Replacing the Laser Driver Panel

Purpose
The laser driver panel can be disassembled and replaced by following the procedures specified in
Tools/Spare Parts
 2.5mm hex-socket screwdriver
 New laser driver panel
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5 Optical System
Preliminary Steps
Disassembly
1. Use a 2.5mm hex-socket screwdriver to disassemble the four M3×6 stainless-steel inner hex
screws that affix the baffle plate of the laser driver panel.
2. Pull out the two wires from the laser driver panel.
3. Pull out the two wires from the laser driver panel. Use a #2 (Ph2) Phillips screwdriver to
disassemble the four M3×8 cross-recessed pan-head combination screws, and carefully detach
the laser driver panel.
Figure 5-4 Replacing the Laser Driver panel
1- M3×6 stainless-steel inner hex 2-Baffle plate of the laser driver

screw (×4) panel
3- M3×8 cross-recessed pan-head 3-Laser driver panel

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5 Optical System
Installation
LED.
5.4 Fine Tuning of the Flow Chamber

Purpose
The fine tuning of the flow chamber can be performed by following the procedures specified in this
section; however, in each case, "Preliminary Steps" needs to be taken before you begin.
Tools/Spare Parts
#2 (Ph2) Phillips screwdriver
Preliminary Steps
2. Take down the cover panel of the optical system box.
3. Turn on the device and launch the software application; then enter the Optical Tuning screen.
Tuning Procedures
1. Use a #2 (Ph2) Phillips screwdriver to loosen the two M3×8 cross-recessed pan-head
combination screws that affix the locking plates of the flow chamber.
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5 Optical System
Figure 5-5 Fine tuning of the flow chamber
1- M3×8 cross-recessed pan-head 2- Adjustment knob of the flow chamber

2. Shake the 7um nominal particles well, and use the 7um nominal particles as a sample for DIFF
testing.
 If the resultant scattergram shows a square or diamond with the upper and lower sides
parallel to each other, the device does not need fine-tuning.
 If the scattergram shows that the upper and lower sides are not parallel, then take the next
step.
3. If the slanting directional arrow shown in the scattergram is ↗, then rotate the adjustment knob of
the flow chamber counter-clockwise; if the slanting direction shown in the scattergram is ↘, then
rotate the adjustment knob of the flow chamber clockwise (the adjustment knob should be lightly
rotated).
4. Use the 7um nominal particles as a sample for DIFF testing.
 If the resultant scattergram shows a square or diamond with the upper and lower sides
parallel to each other, the device does not need fine-tuning.
 If the scattergram shows that the upper and lower sides are not parallel, then repeat Step 3.
5. After tuning, tighten the two locking screws of the flow chamber, and reinstall the cover panel of
the optical system box as well as the four affixing screws; then lock them in place.
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5 Optical System
5.5 Common Problems and Solutions

Problem Description Possible Cause Solution
There is no scattergram  The laser driver Check if the laser is working.

signal when testing the panel is not  If not, replace the laser driver panel.
DIFF signal of venous working.
blood.  If the laser is working, check the power
 The optical supply of the optical preamp panel and
preamplifier panel is replace the optical preamp panel.
not working.
One or some of the Optical preamplifier Check if the laser is working.

signals from the three panel problem  If not, replace the laser driver panel.
angles are 0 when testing
the DIFF signal of venous  If the laser is working, check the power
blood. supply of the optical preamp panel and
replace the optical preamp panel.
If the problem persists after the
replacement, replace the main control
panel.
There are only sporadic  The flow chamber is Check if the collecting corner plate has two
background scattergram dislocated. bilaterally symmetric light spots in the shape
signals when testing the  The hydraulics have of red vertical stripes.
DIFF signal of venous no sheath flow.  If there is only one red vertical stripe or
blood. the two stripes are significantly
different in brightness, make small
adjustment to the location of the flow
chamber and return the
nominal-particle scattergram to normal.
 If there are two red vertical stripes
which are not significantly different in
brightness, check if the pinch valve is
working; if it is, check if Valve 10, 11,
12, 13, and 18 are also working.
The scattergram signals The laser is burnt out. Replace the laser.
are basically normal but
compressed in size when
testing the DIFF signal of
venous blood.
The neutral-particle signal The flow chamber is Make a small adjustment to the location of
(the long horizontal stripe dislocated. the flow chamber and return the
at the top right corner) in nominal-particle scattergram to normal.
the scattergram is
considerably slanted and
the three signals are
mostly overlapping with
inaccurate classification
when testing the DIFF
signal of fresh venous
blood.
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5 Optical System
Problem Description Possible Cause Solution
There is a lot of noise  The reagent is  If the background noise has a certain
when testing the contaminated. shape and is concentrated at the lower
background signal.  Tiny air bubbles get half of the scattergram, keep the DIFF1
into the hydraulics. and DIFF2 fluids at room temperature
and perform three rounds of the
reagent replacement sequence each
for the diluent, DIFF1 and DIFF2.
 If the background signal has no shape
and randomly spreads all over the
scattergram, replace the sheath flow
syringe.
If the problem persists, replace the optical
assembly.
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6 Repairs
6 Repairs
6.1 Introduction
The service engineer can repair the analyzer using standard tools. See the following section for
repairing procedures (including the tools needed).If any repairing step requires a validating step, the
service engineer should strictly follow the procedure and take the validating step.
When following the repairing procedure as specified in this section, the operator should put on
rubber gloves and clean both hands with disinfectant after performing any repair work.
6.2 Preparatory Work Before Repairs
6.2.1 Open the Left Side Door
Purpose
While performing regular machine maintenance and inspection, the left-side door needs to be
opened to disassemble the µl syringe assembly, diluent syringe assembly, lyse syringe assembly,
fluid-level detecting assembly, power switch assembly, driver panel assembly, or main control panel
assembly, which are all in the left section of the machine.
Tools/Spare Parts
Slot-type screwdriver
Opening
Insert the slot-type screwdriver into the slot in the left side door lock and rotate 90°counterclockwise,
then manually open the left side door.
6.2.2 Open the Right Side Door
Purpose
While performing machine maintenance and inspection, the right side door needs to be opened to
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6 Repairs
disassemble the DIFF reaction bath assembly, impedance counting reaction bath assembly (WBC &
RBC), positive-pressure pump, positive-pressure chamber, negative-pressure chamber, or valve-
and pump-specific components, which are all in the right section of the machine.
Tools/Spare Parts
Slot-type screwdriver
Opening
Insert the slot-type screwdriver into the slot in the right side door lock and rotate 90°
counterclockwise, then manually open the right side door.
6.2.3 Opening the Top Panel Cover
Purpose
While performing regular machine maintenance and inspection, the top panel cover needs to be
removed whenever disassembling the sample probe or cleaning the swab, sampling assembly,
autoloader key panel, or indicator light panel.
Tools/Spare Parts
N/A
Preliminary Steps
1. Turn off the power switch on the left side of the device and pull out the power cord plug from the
back panel of the device.
2. Open the left and right side doors.
Opening
1. Remove the door on the right side and find the lock lever on the top panel cover at the back of
the front panel as shown in Figure 6-1. Push the lock lever in the direction of the arrow to make it
detach from the front panel, and then open the top panel cover.
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6 Repairs
Figure 6-1 Opening the Top Panel Cover
2. Turn the top panel cover until the support rod on the door cover locks in place.
6.2.4 Disassembling the Base Panel Cover
Purpose
While performing regular machine maintenance and inspection, the base panel cover needs to be
removed whenever disassembling the sheath flow syringe or mixing components, sample transfer
units, etc.
Tools/Spare Parts
#2 (Ph2) Phillips screwdriver
Preliminary Steps
2. Open the left and right side doors.
3. Remove the top panel cover.
Disassembly
1. When the sample probe is not on the top, move the vertical slide of the sample probe upward to
the top first to ensure that the sample probe will not be damaged or do any injury to maintenance
personnel.
2. Remove the four M3×8 cross-recessed countersunk head screws which affix the front base
panel cover, and then remove the front base panel cover as shown in the figure below.
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6 Repairs
1-base panel cover 2-M4×8 cross-recessed countersunk

head screws (×4)
6.3 Replacing the Sampling Assembly
6.3.1 Replacing the Sample Probe
Purpose
The sample probe can be disassembled and replaced by following the procedures specified in this
section; however, in each case, "6.2 Preparatory Work Before Repairs" needs to be taken before you
begin.
Tools/Spare Parts
 Sample probe
Disassembly
1. When the sample probe is not at the top, move the vertical slide of the sample probe upward until
it is on top.
2. Move the sample probe assembly horizontally to the approximate position as indicated in Figure
6-2 and make sure there is enough space to detach the swab from the sample probe.
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6 Repairs
3. Pull out the swab's snap ring to detach it from the lower bracket panel. See Figure 6-2.
Figure 6-2 Replacement of sample probe (1)
1- Cleansing swab 2- Swab circlip
3-Vertical slide of the

sample probe
4. Use a 2.5mm hex wrench to remove the two M3×8 stainless steel inner hex screws from the
pressure plate, and then remove the pressure plate and the sample probe. See Figure 6-3.
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Figure 6-3 Replacement of sample probe (2)

1
1
2
2
3
3
1-Sample probe 2-Pressure plate affixing the sample probe
3- M3×8 stainless-steel inner hex screw (×2)
6.3.2 Replacing the Optocoupler
Purpose
The optocoupler can be disassembled and replaced by following the procedures specified in this
begin.
Tools/Spare Parts
 2.5mm hex wrench
 Optocoupler
Disassembly
Use the 2.5mm hex wrench to remove the M3 hex screw affixing the optocoupler, then pull out the
plug attached to the optocoupler wire to disassemble the optocoupler to be replaced.
6.3.3 Replacing the Sampling Assembly in X- or Y-direction
Purpose
The sampling assembly can be disassembled and replaced by following the procedures specified in
this section; however, in each case, "6.2 Preparatory Work Before Repairs" needs to be taken before
you begin.
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Tools/Spare Parts
 diagonal cutting pliers
Disassembly
1. Use the diagonal cutting pliers to cut off all the nylon binding tapes affixing the fluid tubes
(aspiration tubes of the sample probe and swab tubes), then pull out the fluid tubes of the sample
probe and the swab to detach them from the sampling assembly.
2. Pull out all the motor wires and optocoupler plug from the horizontal moving module of the
sampling assembly, and use a #2 (Ph2) Phillips screwdriver to take out the three M3×6 cross
recessed countersunk head screws affixing the bracket for drag chains (see Figure 6-4) to
detach the sampling-specific drag chain from the sampling assembly.
Figure 6-4 Replacing the sampling assembly in X- or Y-direction (1)
1- tank drag chain 2- M3×6 cross-recessed countersunk-head screw

connector (×3)
3. Use #2 (Ph2) Phillips screwdriver to remove the four M4×10 cross-recessed pan-head
combination screws which affix the sampling assembly module and carefully move the sampling
assembly module outward from the machine. In the process of moving the module outward, the
drive motor in the horizontal motion direction of the sampling assembly, the optocoupler cables,
and connectors should all be carefully drawn through their corresponding holes in the machine.
After that, all the connecting plugs should be pulled out so that the sampling component module
can be completely removed.
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Figure 6-5 Replacing the sampling assembly in X- or Y-direction (2)
1-Sampling assembly 2- M4×10 cross-recessed pan-head combination screw (×4)
6.4 Replacing the Power Assembly

Purpose
The power assembly can be disassembled and replaced by following the procedures specified in this
begin.
Tools/Spare Parts
 Power assembly with the same specifications
Preliminary Steps
Turn off the power switch on the left side of the device and pull out the power cord plug from the back
panel of the device.
Disassembly
1. Use a #2 (Ph2) Phillips screwdriver to disassemble the five M3×8 cross-recessed pan-head
combination screws affixing the back panel on the back panel cover of the device. See Figure
6-6.
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Figure 6-6 Replacement of power assembly (1)
1-Power assembly 2- M3×8 cross-recessed pan-head

2. Carefully pull out the power assembly and unplug all the connectors attached to the lead wire of
the power assembly.
3. Detach the power source to be replaced from the power assembly and install the new power
source.
Figure 6-7 Replacement of power assembly (2)
1-Power source 2-Bracket panel of the power source
3-Back panel of the power source 4- M3×6 cross-recessed countersunk-head screw (×10)
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6.5 Replacing the Hydraulics Components incl. Valves,

Pumps, and Pressure Chambers
6.5.1 Replacing the Valve Assembly
Purpose
The optical assembly can be disassembled and replaced by following the procedures specified in
this section; however, in each case, “Preliminary Steps” needs to be taken before you begin.
Tools/Spare Parts
 The replacement valve with the same specifications
Preliminary Steps
3. If it is only the electromagnetic valve on the left or right clapboard that needs to be replaced,
open the corresponding left- or right-side door; if the one on the front panel needs to be replaced,
remove the front panel cover by following the instructions in "6.2.3 Opening the Top Panel
Cover".
Disassembly
1. Disassemble the peripheral fluid tubes connected to the valve assembly.
2. Use a #2 (Ph2) Phillips screwdriver to disassemble the two M3×8 cross-recessed pan-head
combination screws which affix the valve assembly, and carefully take out the valve assembly
while making sure to unplug the attached wires.
Figure 6-8 Replacing the valve assembly
1- the corresponding replacement valve 2-Installation panel affixing the valve
3- M3×8 cross-recessed pan-head

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Installation
 Make sure to the use correct model of valve and create a reliable connection
 This machine involves a great number of valves. Only one valve is discussed as an example
here, and all the other valves follow the same maintenance procedures.
6.5.2 Replacing the Air Pump Assembly
Purpose
The air pump assembly can be disassembled and replaced by following the procedures specified in
Tools/Spare Parts
 The replacement air pump with the same specifications
Preliminary Steps
3. Open the right side door.
Disassembly
1. Pull out the peripheral fluid tubes and wire connectors attached to the air pump.
combination screws which affix the air pump, and carefully take out the air pump assembly. See
the picture below for details.
Figure 6-9 Replacing the air pump assembly
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1- the corresponding replacement air 2-Installation panel for the

pump air pump
3- M4×6 cross-recessed pan-head 4-Binding tape

3. Cut off the binding tape affixing the air pump to the installation panel and detach the air pump.
Installation
 Make sure that the binding tapes contribute to a reliable connection so as to prevent the air
pump from shaking and loosening while in operation.
 Pay special attention to the tubing joints and ensure that the connections are sound.
Resetting the Machine

1. Close the right side door.
2. Plug the power cord into the back panel of the device and turn on the power switch on the left
6.5.3 Replacing the Liquid Pump Assembly
Purpose
The liquid pump assembly can be disassembled and replaced by following the procedures specified
in this section; however, in each case, "6.2 Preparatory Work Before Repairs" needs to be taken
before you begin.
Tools/Spare Parts
 The replacement liquid pump with the same specifications
Preliminary Steps
Disassembly
1. Pull out the peripheral fluid tubes and wire connectors attached to the liquid pump.
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2. Use a #2 (Ph2) Phillips screwdriver to loosen the two M4×10 cross-recessed pan-head
combination screws which affix the liquid pump, and move the liquid pump assembly to the left till
the big hole on the affixing panel of the liquid pump is aligned to the affixing screw, then carefully
hold up the liquid pump assembly to take it out. See the picture below for details.
Figure 6-10 Replacing the liquid pump assembly (1)
2 1
2
1- liquid pump assembly 2- M4×10 cross-recessed pan-head combination screw (×2)
3. The further dismantling of the liquid pump assembly is shown in the picture below.
Figure 6-11 Replacing the liquid pump assembly (1)
1- liquid pump 2- M3×8 stainless-steel inner hex screw (×8)
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Installation
 All the wirings need to be set up according to their original positions to prevent them from being
crushed or otherwise damaged, and to prevent the working liquid pump from being shaken,
which affects the fluid tubes.

6.5.4 Replacing the Positive-pressure Chamber Assembly
Purpose
The positive-pressure chamber assembly can be disassembled and replaced by following the
procedures specified in this section; however, in each case, "6.2 Preparatory Work Before Repairs"
needs to be taken before you begin.
Tools/Spare Parts
 The replacement parts or assembly of the positive-pressure chamber with the corresponding
specifications
Preliminary Steps
Open the right side door.
Disassembly
1. Disassemble the peripheral fluid tubes connected to the positive-pressure chamber.
combination screws which affix the positive-pressure chamber, and carefully take out the
positive-pressure chamber assembly. See the picture below for details.
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Figure 6-12 Replacing the positive-pressure chamber assembly (1)
1-Positive-pressure 2- M4×10 cross-recessed pan-head

chamber assembly combination screw (×2)
3. The further dismantling of the positive-pressure chamber assembly is shown in the picture below.
Figure 6-13 Replacing the positive-pressure chamber assembly (2)
1-Fixation panel for the positive- pressure chamber 2-Main body of the pressure
chamber
3-O-shaped seal ring 4-Cover of the pressure chamber
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Installation
 Make sure to place the seal ring into the seal tank to keep the pressure chamber airtight.
 The seal tube attached to the bottom joint of the pressure chamber should not be subject to any
leakage.
 All the tubing needs to be set up according to the relevant standards or the original positions of
the machine components to prevent them from being crushed or otherwise damaged.

Close the right side door.
6.5.5 Replacing the Negative-pressure Chamber Assembly
Purpose
The negative-pressure chamber assembly can be disassembled and replaced by following the
procedures specified in this section; however, in each case, "6.2 Preparatory Work Before Repairs"
needs to be taken before you begin.
Tools/Spare Parts
 The replacement parts or assembly of the negative-pressure chamber with the corresponding
specifications
Preliminary Steps
Open the right side door.
Disassembly
1. Disassemble the peripheral fluid tubes connected to the negative-pressure chamber.
combination screws which affix the negative-pressure chamber, and carefully take out the
negative-pressure chamber assembly. See the picture below for details.
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Figure 6-14 Replacing the negative-pressure chamber assembly
1-Negative-pressure 2- M4×10 cross-recessed pan-head

chamber assembly combination screw (×2)
3. The further dismantling of the negative-pressure chamber assembly is the same as that of the
positive-pressure chamber assembly.
Installation
 Make sure to place the seal ring into the seal tank to keep the pressure chamber airtight.
 All the tubing needs to be set up according to the relevant standards or the original positions of

Close the right side door.
6.6 Replacing the Sheath Flow Syringe Assembly
6.6.1 Replacing the Syringe
Purpose
The syringe can be disassembled and replaced by following the procedures specified in this section;
however, in each case, "Preliminary Steps" needs to be taken before you begin.
Tools/Spare Parts
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6 Repairs
 The replacement syringe module with the same specifications
Preliminary Steps
 Open the left side door when replacing the μl syringe, diluent syringe, or lyse syringe on the left
clapboard.
 The base panel cover should be removed before replacing the sheath fluid syringe on the front
panel.
Disassembly
1. Pull out the peripheral fluid tubes connected to the syringe module to be replaced.
2. Use the 2.5mm hex-socket screwdriver to disassemble the two M3×8 stainless-steel inner hex
screws affixing the syringe module. Then take out the syringe module. See the picture below (the
module shown here is for lyse syringe).
Figure 6-15 Replacing the syringe
1- Syringe module 2- M3×8 stainless-steel inner hex screw (×2)
Installation
6.6.2 Replacing the Motor
Tools/Spare Parts
 The replacement syringe module with the same specifications
Preliminary Steps
 Open the left side door when replacing the μl syringe, diluent syringe, or lyse syringe on the left
clapboard.
 Follow the procedure for dismantling the front panel cover when replacing the sheath flow
syringe on the front panel.
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6 Repairs
Disassembly
1. Pull out the peripheral fluid tubes connected to the syringe module to be replaced.
2. Use the 2.5mm hex-socket screwdriver to disassemble the four M3×8 combination screws
affixing the syringe module. Then take out the syringe module. See the picture below (the
module shown here is for lyse syringe).
3. Take out the two M3×8 inner hex screws on the shield cover, then remove the white bolt from the
motor. Next, remove the four M3×8 inner hex screws from the back of the motor for the
replacement.
1- Motor bolt 2-Motor
Installation
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6.7 Replacing WBC and RBC Bath Assemblies
6.7.1 Dismantling and Replacing the WBC Bath Assembly
Purpose
The WBC counting bath assembly can be disassembled and replaced by following the procedures
specified in this section; however, in each case, "Preliminary Steps" needs to be taken before you
begin.
Tools/Spare Parts
 The replacement WBC counting bath assembly with the same specifications
Preliminary Steps
1. Run the draining sequence of the software to drain any residual liquid inside the machine.
Disassembly
1. Use a #2 (Ph2) Phillips screwdriver to disassemble the M3×8 cross-recessed pan-head
combination screw in the lower section of the shield cover to remove the cover; to take out the
screw, move slightly upwards for 3mm or so and apply force in a direction perpendicular to the
right clapboard.
Figure 6-16 Dismantling and replacing the WBC bath assembly (1)
1- Shield cover of the counting 2-Cross-recessed pan-head combination

bath screw
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2. Pull out the peripheral fluid tubes attached to the WBC counting bath assembly. Pay attention to
handling the residual fluid; try to prevent it from flowing onto the other components in the
machine, and wipe clean if necessary.
combination screw for grounding on the middle clapboard of the shield case cover and pull out
the grounding wire.
1- WBC counting chamber 2- M3×8 cross-recessed pan-head combination

assembly screw
4. Use 2.5mm hex wrench to remove the two M3×8 stainless-steel hex-socket screws affixing the
upper section of WBC counting bath assembly.
1- WBC counting 2- M3×8 stainless-steel inner hex screw

chamber assembly (×2)
5. Carefully and slowly take out the WBC counting bath assembly. Pay attention to the wiring in the
process of moving to avoid breaking the wires.
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6 Repairs
6. Remove the protective ring from the shield case and open the wire clip to take out the inner wire
along the stretching direction of the wire. Then take out the WBC counting bath assembly as a
whole.
7. The further dismantling of the WBC counting chamber assembly. See the picture below for
details.
1- LED 2-Front counting bath
3-Flat rubber gasket 6*4.5*0.5 4-WBC aperture
5-Flat rubber gasket 6*3*0.5 6-O-shaped ring 5.5*1.0
7-Electrode of the rear bath 8-O-shaped ring 6.5*1.0
9-Rear chamber 10-Optical receiver
11-Optical filter of the counting 12-HGB bracket

bath assembly
Installation
 All the wirings need to be set up according to the working procedures or the original positions of
 The aperture, seal gasket, and seal ring need to be correctly positioned and oriented in the right
direction.

1. Reinstall the shield case cover.
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6.7.2 Dismantling and Replacing the RBC Bath Assembly
Purpose
The RBC counting bath assembly can be disassembled and replaced by following the procedures
specified in this section; however, in each case, "Preliminary Steps" needs to be taken before you
begin.
Tools/Spare Parts
 The replacement WBC counting bath assembly with the same specifications
Preliminary Steps
Disassembly
combination screw in the lower section of the shield cover to remove the cover; to take out the
screw, move slightly upwards for 3mm or so and apply force in a direction perpendicular to the
right clapboard. Refer to the corresponding section in 6.7.1 Dismantling and Replacing the
WBC Bath Assembly.
2. Pull out the peripheral fluid tubes attached to the RBC counting bath assembly. Pay attention to
handling the residual fluid; try to prevent it from flowing onto the other components in the
machine, and wipe clean if necessary.
combination screw for grounding on the middle clapboard of the shield case cover and pull out
the grounding wire.
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Figure 6-20 Dismantling and replacing the RBC bath assembly (1)
1- RBC counting chamber assembly 2- M3×8 cross-recessed pan-head combination

screw
4. Use 2.5mm hex wrench to remove the two M3×8 stainless-steel inner hex screws affixing the
upper section of the RBC counting bath assembly.
1- RBC counting chamber assembly 2- M3×8 stainless-steel inner hex screw (×2)
5. Carefully and slowly take out the RBC counting bath assembly. Pay attention to the wiring during
the process of moving to avoid breaking the wires.
6. Remove the protective ring from the shield case and open the wire clip to take out the inner wire
along the stretching direction of the wire. Then take out the RBC counting bath assembly as a
whole.
7. The further dismantling of the RBC counting chamber assembly. See the picture below for
details.
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1-Front counting bath 2-Flat rubber gasket 6*4.5*0.5
3-RBC aperture 4-Flat rubber gasket 6*3*0.5
5-O-shaped ring 5.5*1.0 6-Electrode of the rear bath
7-O-shaped ring 6.5*1.0 8-Rear chamber
Installation
 The aperture, seal gasket, and seal ring need to be correctly positioned and oriented in the right
direction.

1. Reinstall the shield case cover.
6.8 Dismantling and Replacing the Mix Assembly

Purpose
The mix assembly can be disassembled and replaced by following the procedures specified in this
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6 Repairs
section; however, in each case, "Preliminary Steps" needs to be taken before you begin.
Tools/Spare Parts
 Corresponding mix assembly that needs replacing
Preliminary Steps
2. Perform "6.2 Preparatory Work Before Repairs".
Disassembly
1. Take note of the plug numbers of the corresponding cables for all the motors and the
optocouplers on the mix assembly to ensure proper reconnection, and then pull out all the motor
cables and optocouplers plugs.
2. Use a #2(Ph2) Phillips screwdriver to remove the four M4×10 cross-recessed pan-head
combination screws which affix the mix assembly to the top of the front panel (see the following
figure). Meanwhile, securely hold the mix assembly at the back of the front panel to prevent it
from dropping.
Figure 6-23 Dismantling the mix assembly
1 - Mix assembly 2- Front panel

3. After all the four M4×10 cross-recessed pan-head combination screws are removed, you can
manually remove the mix assembly at the back of the front panel as shown below in the figure.
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6 Repairs
1
1
1 - Mix assembly
4. Further dismantling of the mix assembly—remove the mix jaw as shown in the following figure.
Figure 6-24 Mix assembly--removing the mix jaw
1-jaw arm body 2-jaw torsion spring
3-jaw body (2x) 4-jaw sheet (2x)
5-lower fixed plate on the jaw 6-M3×6 cross-recessed

countersunk-head screw (3x)
7- rubber slip-proof sheet on the jaw 8-M3×8 inner socket screws

(2x)
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9-tube pressure plate on the jaw 10-M4×8 socket set screws
11-jaw yaw axis

5. Further dismantling of the mix assembly--remove the deflection optocoupler as shown in the
following figure.
Figure 6-25 Mix assembly-removing the deflection optocoupler
1-deflection optocoupler fixed block on 2-optocoupler OPB890T11

the jaw
3-M3×8 inner hex screw (2x)

6. Further dismantling of the mix assembly--remove the upper and lower optocouplers as shown in
the following figure.
Figure 6-26 Mix assembly-removing upper and lower optocouplers
1-M3×8 inner hex screw (×4) 2-Upper optocoupler
3-Lower optocoupler
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7. Further dismantling of the mix assembly--remove the front and rear optocouplers as shown in the
following figure.
Figure 6-27 Mix assembly-removing front and rear optocouplers
1-rear optocoupler 2- M3×8 inner hex screw (×4)
3- front optocoupler
6.9 Replacement of DIFF Bath Assembly

Purpose
The DIFF bath assembly can be disassembled and replaced by following the procedures specified in
this section; however, in each case, "6.2 Preparatory Work Before Repairs" and "6.8 Dismantling and
Replacing the Mix Assembly" needs to be taken before you begin.
Tools/Spare Parts
 The corresponding DIFF bath assembly for replacement
Preliminary Steps
Disassembly
1. First pull out the peripheral fluid tubes connected to the DIFF bath assembly and note that there
will be a little residual liquid left in the tubing. Wipe it off with some absorbent such as tissue
paper to avoid contamination or erosion.
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2. Use a Phillips screwdriver to remove the M4×10 cross recessed pan-head combination screw in
the lower section of DIFF bath. Slowly take out the DIFF bath assembly in a direction
perpendicular to the right clapboard. Note that the wiring of the heating plate and the
temperature sensor will be pulled out; try to avoid breaking the wires or damaging the insulation
layer. Unplug the connector to remove the DIFF bath assembly, as shown in the picture below.
Figure 6-28 Replacement of DIFF bath assembly
1- DIFF bath assembly 2-Cross-recessed pan-head combination

screw
Installation
 The back of the lower section of the DIFF bath assembly needs to be pressed against the right
clapboard and no wires or other objects must be between them.
 When placing the DIFF bath assembly, try to prevent impurities from entering the tubing and
causing clogging.

6.10 Dismantling and Replacing the Sample Transfer Unit

Purpose
The driver panel can be disassembled and replaced by following the procedures specified in this
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6 Repairs
begin.
Tools/Spare Parts
 Corresponding sample transfer unit assembly and components for replacement
Preliminary Steps
2. Perform "6.2 Preparatory Work Before Repairs".
Disassembly
1. Removing the switch which detects if the sample rack is loaded in place—Use a Phillips
screwdriver to remove the two M2×10 cross-recessed pan-head screws which affix the micro
switch (see the following figure), unplug the corresponding cables on the switch, and remove the
micro switch. You can then replace the micro switch with spare parts designed for the same
model number.
Figure 6-29 Removing the switch which detects if the sample rack is loaded in place
1- micro switch which detects if the 2- M4×10 cross-recessed pan-head

sample rack is loaded in place combination screw (×2)
2. Removing the switch which detects the presence of test tubes on the sample rack—Use a
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6 Repairs
2.5mm hex wrench to remove the two M3×8 stainless steel inner hex screws which affix the
micro switch on the test tube presence support (see the following figure), unplug the
corresponding cables on the switch, and remove the micro switch. You can then replace the
micro switch with the same specialized spare parts from the manufacturer.
Figure 6-30 Removing the switch which detects the presence of test tubes on the sample rack
1-micro switch which detects the presence 2- M3×8 stainless-steel inner hex
of test tubes on the sample rack screw (×2)
3- test tube presence support

3. Dismantling the sample transfer unit —Take note of the plug numbers of the corresponding
cables for all the motors and optocouplers on the sample transfer unit to ensure proper
reconnection, and then pull out all the motor cables and optocouplers plugs from the sample
transfer unit. Use a #2(Ph2) Phillips screwdriver to remove the five M4x 10 cross-recessed
pan-head combination screws (see the following figure) which affix the transfer unit on the base
panel of the main unit, and then remove the sample transfer unit from the main unit.
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Figure 6-31 Dismantling the sample transfer unit
1-sample transfer unit 2-base panel of the main unit
3-front panel of the main unit 4- M4×10 cross-recessed pan-head

combination screw (5x)
4. Further dismantling of the sample transfer unit—removing the test tube rack workbench
Use a #2(Ph2) Phillips screwdriver to remove the eight M3x 8 cross-recessed pan-head
combination screws (see the following figure) which affix the test tube rack workbench, and then
remove the test tube rack workbench.
Figure 6-32 Further dismantling of the sample transfer unit—test tube rack workbench
1-test tube rack workbench 2- M3×8 cross-recessed pan-head

3-hexagonal copper stud
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6 Repairs
5. Further dismantling of the sample transfer unit—removing the vertical loading assembly of the
test tube rack
Use a #2(Ph2) Phillips screwdriver to remove the four M3×8 cross-recessed pan-head
combination screws (see the following figure) which affix the vertical loading assembly of the test
tube rack, and then remove the vertical loading assembly of the test tube rack.
Figure 6-33 Further dismantling of the sample transfer unit
—loading assembly of the test tube rack
1- vertical loading assembly of the test 2- M3×8 cross-recessed pan-head

tube rack combination screw (×4)
6. Further dismantling of the sample transfer unit—removing the unloading assembly of the test
tube rack
Use a #2(Ph2) Phillips screwdriver to remove the three M3×8 cross-recessed pan-head
combination screws (see the following figure) which affix the unloading assembly of the test tube
rack, and then remove the vertical loading assembly from the sample transfer unit.
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—unloading assembly of the test tube rack
1- unloading assembly of the test tube 2- M3×8 cross-recessed pan-head

rack combination screw (×3)
7. Further dismantling of the sample transfer unit—removing the horizontal feed driver assembly of
the test tube rack
Use a #2(Ph2) Phillips screwdriver to remove the two M3×8 cross-recessed pan-head
combination screws (see the following figure) which affix the conveyor belt pressure plate, and
then remove the conveyor belt pressure plate. Now use a #2(Ph2) Phillips screwdriver to remove
the three M3×8 cross-recessed pan-head combination screws which affix the horizontal feed
driver assembly of the test tube rack, and then remove the assembly from the sample transfer
unit.
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6 Repairs

—horizontal feed driver assembly of the test tube rack
1- M3×8 cross-recessed pan-head 2-conveyor belt pressure plate

3-horizontal feed driver assembly of

the test tube rack
8. Further dismantling of the sample transfer unit—removing the horizontal feed assembly of the
test tube rack
Use a #2(Ph2) Phillips screwdriver to remove the two M4×10 cross-recessed pan-head
combination screws (see the following figure) which affix the horizontal feed assembly of the test
tube rack, and then remove the assembly from the base panel of the sample transfer unit.
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6 Repairs

—horizontal feed assembly of the test tube rack
1-horizontal feed assembly of the 2- M4×10 cross-recessed pan-head

test tube rack combination screw (×2)
3-base panel of the sample

transfer unit
6.11 Replacement of the Main Control Panel

Purpose
The main control panel can be disassembled and replaced by following the procedures specified in
you begin.
Tools/Spare Parts
 Corresponding specifications needed to replace the main control panel
Preliminary Steps
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6 Repairs
3. Open the left side door.
Disassembly
1. Pull out the peripheral wires and connectors attached to the main control panel.
2. As shown in Figure 6-37, remove the two M4×8 cross recessed pan-head combination screws
affixing the pallet for the main control panel. Push the PCB handle to the left and then to the right
so as to detach the plug that connects the main control panel to the rear panel from the socket.
Take out the main control panel assembly along the PCB pallet chute.
Figure 6-37 Replacement of the main control panel
1-Main control panel 2-PCB handle

Installation

1. Close the corresponding left side door.
6.12 Replacement of the Driver Panel

Purpose
The driver panel can be disassembled and replaced by following the procedures specified in this
begin.
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6 Repairs
Tools/Spare Parts
 The replacement driver panel with the same specifications
Preliminary Steps
Disassembly
1. Pull out the peripheral wires and connectors attached to the driver panel.
2. Disassemble the driver panel below the main control panel by referring to the procedure for
disassembling the main control panel.
Installation

6.13 Replacement of Reagent Testing Panel

Purpose
The reagent testing panel can be disassembled and replaced by following the procedures specified
in this section; however, in each case, "6.2 Preparatory Work Before Repairs" needs to be taken
before you begin.
Tools/Spare Parts
 The replacement reagent testing panel with the same specifications
Preliminary Steps
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6 Repairs
Disassembly
1. Remove the four M3×8 cross recessed pan-head combination screws affixing the protective
cover of the reagent testing panel and remove the protective cover.
Figure 6-38 Replacement of reagent testing panel (1)
1- Protective cover 2-M3×8 cross-recessed pan-head

2. Pull out all the exposed peripheral wires connected to the reagent testing panel.
3. Remove the four M3×8 cross-recessed pan-head combination screws affixing the reagent testing
panel and carefully take out the reagent testing panel. Make sure that the metal parts will not
scratch the wiring at the rear of the panel. Pull out the rear wiring to remove the reagent testing
panel.
Figure 6-39 Replacement of reagent testing panel (2)
1- Reagent detection 2- M3×8 cross-recessed pan-head

panel combination screw (×4)
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6 Repairs
Installation
 Pay attention to the position of wires when installing the sensor panel so as to prevent the wires
from breaking.

6.14 Replacing the Sheath-flow Sensor and Temperature

Sensor
6.14.1 Disassembling and Replacing the Sheath-flow Sensor
Purpose
The sheath-flow sensor can be disassembled and replaced by following the procedures specified in
this section; however, in each case, "6.2 Preparatory Work Before Repairs", "6.9 Replacement of
DIFF Bath Assembly" and "6.11 Replacement of the Main Control Panel" need to be taken before
you begin.
Tools/Spare Parts
 The replacement sheath-flow sensor with the same specifications
Preliminary Steps
3. Open the left side door and the front panel cover, and take off the main control panel and the
driver panel.
Disassembly
1. Pull out the first plug at the top from the back of the reagent testing panel, then open the wire
clips for the sheath-flow sensor on the inner wall of the left clapboard to take out the wire.
2. Pull out the tube attached to the sheath-flow sensor and remove it from the wire clip.
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6 Repairs
Installation
Follow the corresponding disassembly steps in reverse order. Plug the wire connector into the
reagent testing panel and re-connect the tubing.

1. Re-install the driver panel, the main control panel and the front panel cover, and close the
corresponding left side door.
6.14.2 Disassembling and Replacing the Temperature Sensor
Purpose
The temperature sensor can be disassembled and replaced by following the procedures specified in
you begin.
Tools/Spare Parts
 2 hex-socket screwdriver
 The replacement temperature sensor with the same specifications
Preliminary Steps
2. Open the left side door and the front panel cover.
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6 Repairs
Disassembly
Loosen the screws in the bracket for temperature sensor to take the sensor out. Then replace it with
a new sensor and tighten the screws.
Figure 6-40 Disassembling the temperature sensor
1-Temperature sensor 2-Temperature sensor bracket
3-M3×8 fixation screws
Installation

1. Re-install the front panel cover, and close the corresponding left side door.
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7 Software Installation and Upgrade
7.1 Software Installation
7.1.1 Preparation
Confirm the PC configuration.
RAM ≥2G
Hard disk ≥20G
CPU ≥1.4G
Graphics Card OpenGL 2.0 or above
Display screen Display aspect ratio: 10:6

Resolution: ≥1280*768
Operation system Windows XP/Windows 7/Windows 8/Windows 8.1/Windows 10
7.1.2 Procedure for Software Installation

1. Insert the installation CD into the CD drive of the PC, then browse the PC folders to find the CD
directory. Double-click the software "Setup.exe", and a dialogue box will be displayed with
command prompt information. Choose the installation language and click OK (see Figure 7-1).
Figure 7-1 Choose the installation language
2. In the subsequent dialog boxes, keep clicking "Next", then select "Install" in the following screen
(see Figure 7-2).
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Figure 7-2 Confirm the installation
3. At last, click "Finish" button to complete the software installation (see Figure 7-3).
Figure 7-3 Complete the software installation
138
7.1.3 Troubleshooting Software Installation

If the installation process fails or a Net Framework installation exception is identified after installation,
manually download Net Framework 4.0 software for installation. After the installation is complete,
repeat the procedures in "7.1.2 Procedure for Software Installation" to complete software installation.
7.2 Software Upgrade
7.2.1 Upgrading the Main System

1. Double click "upgradestudio.exe" to launch the upgrade tool.
It takes about half a minute for the upgrade tool to connect to the machine. Please be patient.
2. Click the "Open File" button, then select and open the upgrade file
"upgrade_1105_vxxxx_XXXXXXXXXXXX.tar" in the popup dialog box.
"xxxx" indicates the version of the upgrade file, and "XXXXXXXXXXXX" indicates the release
date of the upgrade file.
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3. Click "Upgrade".
The upgrade confirmation dialog box will pop up.
4. Click "Yes".
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The successful upgrading of the upgrade tool is shown in the picture below.
5. After it is successfully upgraded, power off the device and then close the upgrade tool.
7.2.2 Upgrading the Software Interface

Note: When upgrading the user interface, the current version needs to be closed; otherwise the
upgrade will not be successful.
1. Launch the IPU upgrade tool, select the language, and click "OK".
2. Keep clicking "Next," and then select "Install" in the screen as shown below.
141
3. Click "OK".
4. Click "OK" to continue the upgrade.
5. Finally, click "Finish" to complete the upgrade.
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7.2.3 Troubleshooting Host Upgrade

Error message Cause analysis Solution
Invalid upgrade file. Incorrect copy of upgrade Re-download the upgrade

file. file.
Extract upgrade file fail.
Upgrade file name was
changed.
Action timeout Server connection Check if the IP address of

timeout. the host is correct.
Upgrade action timeout Check if there is anything
while in the process of wrong with the host
upgrade. network connection.
All versions are the same, no need to upgrade All version numbers, Exit the upgrade program.
before and after upgrade,
are the same and no
upgrade is required.
Data upgrade fail, version is XXX XXX version's data Exit and restart the
compatibility process fails upgrade.
while the application
program is being
upgraded.
Create app tar file fail The model of the main Replace with the upgrade
unit program does not package that correctly
match that of the upgrade corresponds to the main
package. unit.
Ssh run script Upgrading bootstrap Restart the main unit and
program fails. try to upgrade again.
/dymind/app/upgrade/script/up_uboot.sh fail
Ssh run script Upgrading kernel program Restart the main unit and
fails. try to upgrade again.
/dymind/app/upgrade/script/up_kernel.sh fail
Ssh run script Upgrading main control Restart the main unit and
panel FPGA fails. try to upgrade again.
/dymind/app/upgrade/script/up_main_fpga.sh
fail
Ssh run script Upgrading driver panel Restart the main unit and
FPGA fails. try to upgrade again.
/dymind/app/upgrade/script/up_driver_fpga.sh
fail
Ssh run script Upgrading driver panel Check if the dial-up status
MCU fails. of the driver panel board
/dymind/app/upgrade/script/up_driver_mcu.sh is OFF.
fail
Restart the main unit and
try to upgrade again.
Ssh run script Upgrading autoloader Restart the main unit and
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Error message Cause analysis Solution

/dymind/app/upgrade/script/up_as_fpga.sh panel FPGA fails. try to upgrade again.
fail
Ssh run script Upgrading autoloader Check if the dial-up status

panel MCU fails. of the driver panel is OFF.
/dymind/app/upgrade/script/up_as_mcu.sh fail
Restart the main unit and
try to upgrade again.
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8 Comprehensive Device Debug
Since the replacement and maintenance of some components can lead to changes in relevant tuning
parameters, this section introduces the tuning procedures for the parameters that may be affected.
8.1 Position Adjustment

Note: The swab height needs to be reset after the replacement of the sampling assembly and
relevant parts of the sampling swab.
1. Select "Service > Position Adjustment", and then click "Swab Position".
2. Click "Start" and check the distance between the bottom of the sample probe and the bottom of
the swab. If the sample probe is lower than the swab, click "Single Step Up"; if higher, click
"Single Step Down".
Each click will move the sample probe slightly. In the end, the bottom of the sample probe will be
aligned with the bottom of the swab.
3. Click "OK".
4. Click "Check" to run the initialization of the sample probe. Check if the bottom of the sample
probe is still aligned with the bottom of the swab. If it is, click "OK" to complete the tuning; if not,
repeat Steps 2~4.
8.2 Mix Position Adjustment

Note: Mix position debugging is required after the replacement of the mix assembly and its
relevant parts.
1. Place the empty test tube rack at the loading position, select "Service > Position Adjustment >
Mix Position", and click "Start ".
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2. Click "Prepare" when the mix assembly stops running.

3. Clip the test tube in the jaw when the jaw stops going down, then check if the tube is upright. If it
is not, click the "Single Step Left" or "Single Step Right" button and adjust it until it is upright.
4. Observe if the bottom of the test tube is aligned with the test tube rack or hole. If it is not, move
the mix assembly left or right to make them aligned and then tighten up the four M4×10
combination screws which affix the assembly.
5. Click the "Single Step Left" or "Single Step Right" button, confirm that the center of the test tube
146
is basically in line with that of the test tube rack, and then click "OK".
6. Place the test tube at position No.10 of the test tube rack, and then place the test tube rack onto
the loading platform. Click "Check" and observe whether there is anything wrong with the test
tube grip and placement. If the position has deviated, start the debugging process over.
8.3 Barcode Scanner Debugging

Note: Position debugging is required after the replacement of the barcode scanner and its
relevant parts.
1. Place a tube with a legible barcode at position No. 10 of the empty test tube rack with the
barcode directly facing the opening of the test tube rack. Then place the test tube rack onto the
loading platform, select "Service > Position Adjustment > Scan Position", and click "Start
Debugging".
2. Observe if the beam of the barcode scanner is exactly in the center of the opening of the test
tube rack. If it is not, adjust the position of the scanner and tighten up the two M3×8 combination
screws which affix the scanning assembly.
3. Place a test tube with a barcode at position 10-1 of the test tube rack with the barcode directly
facing the opening of the test tube rack, and then click "Start Debugging". A "click" sound
indicates the scanning has been successful. If you hear no "click" sound, please debug the
scanner again or check its quality.
8.4 HGB Voltage Gain Setting

The HGB voltage gain setting needs to be performed for the replacement of WBC bath, HGB
wire and main control panel.
1. In the "Setup > Host Settings" screen, click "Gain Settings".
2. Click the up/down adjustment button after the filling blank for HGB value and HGB background
voltage value will change accordingly within 4.5+/0.3V.
3. Click "OK" (see Figure 8-1).
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Figure 8-1 HGB voltage gain setting
8.5 Gain Calibration

Note: Gain calibration needs to be performed after the replacement of WBC bath, RBC bath,
and main control panel.
1. Click "Service > Gain calibration" to access the Gain calibration screen. See Figure 8-2.
Figure 8-2 Gain calibration
2. Fill in the "Target" cells corresponding to W-MCV and MCV with the reference values for quality
control.
3. Perform the QC test for three times in a row for the first run.
The results for each time will be automatically displayed.
 If the CV falls within reasonable parameters, the screen will show the CV and Gain values for
the first run. Go to step 4.
 If it does not fall within reasonable parameters, then the system will delete the previous
results. Please repeat this step.
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4. Perform the QC test for three times in a row for the second run.
The results for each time will be automatically displayed.
 If the CV falls within reasonable parameters, the screen will show the CV and Gain values for
the second run, and show the final results. Please click "OK" to complete gain calibration.
 If it does not fall within reasonable parameters, then the system will delete the previous
results. Please repeat this step for gain calibration.
8.6 Calibration of Calibrators

Note: The calibrators need calibration after the replacement of the WBC bath, the RBC bath,
and the main control panel.
8.6.1 Calibration in Whole-blood Mode

1. In standby mode, click "Cal" to enter the calibration screen.
2. Select "Calibrator" (see Figure 8-3).
Figure 8-3 Calibration of calibrators
3. Input the calibrator lot No. "JZQX-01" in the text box for "Lot No.", and click the "Exp. Date"
control to set the expiry date of the calibrator.
4. Input the target value of the current calibrator parameter.
The calibration mode is "Whole Blood" by default, so there is no need to set the mode.
5. Set the well-shaken calibrator under the sample probe, then press the aspiration key on the
analyzer or "Start" button on the screen to start the calibration counting.
6. Repeat Step 5 for a total of 12 times to get 12 results of calibration counting.
 After the counting is complete, a dialog box will pop up indicating that the test is complete.
Click the "Save" button to save the calibration result.
 If significant differences are found across results, a dialog box for data abnormality will pop
up. Please redo the calibration.
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8.6.2 Calibration in Predilute Mode

1. In standby mode, click "Cal" to enter the calibration screen.
2. Click "Mode & ID" at the bottom right corner and select "Predilute" in the popup dialog box. Then
select "OK".
3. Input the calibrator lot No. "JZYXS-01" in the text box for "Lot No.", and click the "Exp.Date"
control to set the expiry date of the calibrator.
4. For calibration using the prediluted calibrators, please refer to 8.6.1 Calibration in Whole-blood
Mode steps 4~6 in whole-blood mode.
8.7 LIS Connection

If the analyzer needs to be connected to laboratory information system (hereinafter referred to as
LIS), you can complete the connection by following the steps in this section.
8.7.1 Preparation
In order to connect to LIS, please make sure:
 The analyzer main unit and software have been installed successfully.
 The computer installed with analyzer software has equipped with double NIC (10/100M adaptive
network card).
8.7.2 Installing LIS Workstation

1. Install LIS workstation and set instrument type and model.
2. Enter LIS workstation network setup interface after installation and set monitoring IP address and
port number.
Refer to Description of LIS Communication Protocol for Dymind Hematology Analyzers to

complete the support of the LIS workstation to the LIS communication Protocol.
8.7.3 Connecting the Analyzer Main Unit and Software

1. Start the analyzer.
2. Taking Window 7 operating system as an example, click the network connection icon ( ) on
the desktop of the system where the analyzer software has been installed and click the Open
Network and Sharing Center on the pop up box.
The system launches the screen as shown in Figure 8-1.
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Figure 8-1 Network and Sharing Center
3. Click Change adapter settings on the right side of the interface to access the Network
Connections interface, as shown in Figure 8-2.
Figure 8-2 Network Connections
4. Right click on the network card to be set and click Properties in the pop-up shortcut menu.
151
Figure 8-3 Analyzer Network Connection Properties
5. Select Internet Protocol Version 4 (TCP/IPv4), and then click Properties.

The interface as shown below pops up on the screen.
Figure 8-4 Setting the IP Address of The Operating System
152
6. Click Use the Following IP address to set IP address as 10.0.0.102, subnet mask as
255.255.255.0; other parameters can be skipped.
The IP address of analyzer is 10.0.0.101. To make sure analyzer software can be connected to the
main unit normally, same network segment should be used in PC as 10.0.0.102 to connect to the
main unit.
7. Click OK to confirm the settings.

8. Check if the connection is successful.
a. Press combination key [Windows+R] to open the Run window.
b. Input cmd, and then click OK.

The cmd.exe window pops up.
c. Input ping 10.0.0.101 command.
10.0.0.101 is the IP address of the analyzer.
d. Check the running results of the Ping cmd.

As is shown below, analyzer software can be connected to the main unit normally.
As is shown below, analyzer software fails to be connected to the main unit.
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Please analyze the connection failure reason according to the data sent by the screen, and
start to reconnect after the solving the problem If the problem still exists, please contact the
hospital network administrator to handle it.
8.7.4 Connecting Analyzer Software with LIS

1. Use a network cable and another network card to connect the computer installed analyzer
software to the LIS local area network.
2. Log on the auto hematology analyzer software; if done, skip this step.
3. Click LIS Communication in the Setup > General Settings interface.
The LIS Communication interface displays. See Figure 8-5.
Figure 8-5 LIS Communication Settings
4. Input the IP address and port of LIS workstation in Network Settings area.
Find the IP address and port of LIS in the network setup interface in the LIS workstation; if IP
address can’t be found, try the method below:
154
a. Enter the operating system of LIS workstation.

b. Press combination key [Windows+R] to open the Run window.
c. Input cmd, and then click OK.
d. Input the ipconfig command into the cmd.exe window popped out.
The interface shows similar content as follows:
The IPv4 address in the red box is the IP address of LIS workstation.
The IP address 192.168.8.44 of the LIS workstation shown as above is used as an example, real IP
should be in the same network segment with LIS server.
5. Click OK to save the settings.

6. Check if the connection is successful.
The LIS icon in the upper right side on the analyzer screen turns from gray to multi-color
, which indicates auto hematology analyzer software is connected to LIS successfully.

If the icon stays gray, the connection fails. Please check if the IP address and port of LIS is
correct and reconnect as the steps above; if the problem still exists, please contact the hospital
network administrator to handle it.
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9 Alarms and Solutions
This section introduces error messages that can appear in the analyzer, possible causes, and
troubleshooting steps to be taken by the operator. If the problem persists after troubleshooting,
please take hardware issues into account and consider replacing the relevant parts or panels.
9.1 Trouble Analysis and Solution

For the following issues, please click the error message box at the bottom right corner of the
software interface, then click "Remove Error" in the popup dialog box. Usually the problem will be
automatically resolved; if it persists, refer to the "Solution" column for further maintenance.
Problem Solution
No. Problem Description
Name
The sample syringe fails to This syringe problem can occur while it is being
leave its initial position. moved. Please refer to the following solution:
The sample syringe fails to 1. Follow the instructions in "6.6 Replacing the
return to its initial position. Sheath Flow Syringe Assembly" to disassemble
the syringe, then remove the dust cover and
The sample syringe takes optocoupler. Plug the optocoupler into the
too many steps to return to connector of the syringe optocoupler. The user
its initial position. enters the "Status > Sensor" screen and covers
the center of the optocoupler with a piece of
The sample syringe is busy. paper. Check if the optocoupler status shown in
the screen is blocked; if yes, then the optocoupler
Sample syringe timeout
is working properly.
The lyse syringe fails to 2. Follow the instructions in "6.6.2 Replacing the
Syringe leave its initial position. Motor" to disassemble the syringe motor and
1 replace it with a new motor. Then go to syringe
problem The lyse syringe fails to
self-test under "Self-test > Syringe"; if the syringe
return to its initial position.
is working, then the maintenance has been
The lyse syringe takes too successful.
many steps to return to its
initial position.
The lyse syringe is busy.
Lyse syringe timeout
The diluents syringe fails to

leave its initial position.
The diluent syringe fails to

156
Problem Solution
Name
The diluent syringe takes

too many steps to return to
its initial position.
The diluent syringe is busy.
Diluent syringe timeout
The sheath syringe fails to

leave its initial position.
The sheath flow syringe

fails to return to its initial
position.
The sheath flow syringe

takes too many steps to
The sheath flow syringe is

busy.
Sheath flow syringe timeout
The horizontal motor fails to 1. The user enters the "Status > Sensor" screen
leave its initial position. and covers the center of the optocoupler with a
piece of paper. Check if the optocoupler status
The horizontal motor fails to shown in the screen is blocked. If it is, then the
return to its initial position. optocoupler is working properly; if not, then refer
The motor fails to move to to "6.3.2 Replacing the Optocoupler" on how to
the WBC position. replace the optocoupler.
2. Run the machine and observe if the synchronous
The motor fails to move to pulley is slipping. If it is, then replace the stopper
the DIFF position. screws of the pulley (you will need to apply
thread adhesive).
The motor fails to move to
Horizontal
the open sampling position. 3. Refer to "6.3.3 Replacing the Sampling Assembly
2 motor in X- or Y-direction" to disassemble the sampling
problem The horizontal motor fails to assembly and make sure the motor wiring is
move to the autoloader secure. If the wiring is OK, then disassemble the
sampling position. motor and replace it with a new one. After the
installation, make proper adjustments to the belt's
The horizontal motor is
tension, then go to the assembly self-test under
busy.
“Self-test > Syringe”. If the assembly is working,
Optocoupler Timeout in the then the maintenance process is OK.
horizontal motor
The optocoupler of the

horizontal motor is not
working properly.
The vertical motor fails to 1. The user enters "Status > Sensor" screen and
Vertical motor leave its initial position. covers the center of the vertical optocoupler with
3 a piece of paper. Check if the corresponding
problem The vertical motor fails to optocoupler status shown in the screen is
return to its initial position. blocked. If it is, then the optocoupler is working
157
Problem Solution
Name
The motor fails to move into properly; if not, then refer to "6.3.2 Replacing the
position to isolate the air Optocoupler" on how to replace the optocoupler.
bubbles. 2. Run the machine and observe if the synchronous
pulley is slipping. If it is, then replace the stopper
The motor fails to move to screws of the pulley (you will need to apply
the DIFF bath position. thread adhesive).
The motor fails to move to 3. Refer to "6.3.3 Replacing the Sampling Assembly
the counting bath position. in X- or Y-direction" to disassemble the motor
from the sampling assembly and make sure
The motor fails to move to motor wiring is secure. If the wiring is OK, then
the open sampling position. disassemble the motor and replace it with a new
one. After the installation, make the proper
The vertical motor fails to
adjustments to the belt tension, then go to the
move to the pre-piercing
assembly self-test under “Self-test > Syringe”. If
position.
the assembly is working, then it has been
The vertical motor fails to successfully fixed.
move to the autoloader
sampling position.
The vertical motor is busy.
Vertical motor timeout
The optocoupler of the

vertical motor is not working
properly.
The loading motor fails to 1. The user enters "Status > Sensor" screen and
leave its initial position. covers the center of the vertical optocoupler with
a piece of paper. Check if the corresponding
The loading motor fails to optocoupler status shown in the screen is
Loading motor action properly; if not, then refer to 6.10 Dismantling and
overtime. Replacing the Sample Transfer Unit on how to
replace the optocoupler.
Loading motor busy. 2. Run the machine and observe if the synchronous
Loading The optocoupler of the
screws of the pulley (you will need to apply
4 assembly loading motor is not working
thread adhesive).
problem properly.
3. Refer to "6.10 Dismantling and Replacing the
The loading assembly is not Sample Transfer Unit" to disassemble the motor
at its initial position. from the loading assembly and make sure motor
wiring is secure. If the wiring is OK, then
Loading assembly busy.
disassemble the motor and replace it with a new
Loading fails. one. After the installation, make the proper
assembly self-test under "Self-test > Other
Loading action overtime. Self-test". If the assembly is working, then it has
been successfully fixed.
Feeding The feeding motor fails to 1. The user enters "Status > Sensor" screen and
5
assembly leave its initial position. covers the center of the vertical optocoupler with
158
Problem Solution
Name
problem The feeding motor fails to a piece of paper. Check if the corresponding
return to its initial position. optocoupler status shown in the screen is
blocked. If it is, then the optocoupler is working
Feeding motor action properly; if not, then refer to 6.10 Dismantling and
overtime. Replacing the Sample Transfer Unit on how to
Feeding motor busy.
The optocoupler of the pulley is slipping. If it is, then replace the stopper
feeding motor is not working screws of the pulley (you will need to apply
properly. thread adhesive).
3. Refer to "6.10 Dismantling and Replacing the
The feeding assembly is not
Sample Transfer Unit" to disassemble the motor
at its initial position.
from the loading assembly and make sure motor
Feeding assembly busy. wiring is secure. If the wiring is OK, then
Feeding failed. one. After the installation, make the proper
Feeding action overtime. Self-test”. If the assembly is working, then it has
6 The unloading motor fails to 1. The user enters "Status > Sensor" screen and
leave its initial position. covers the center of the unloading optocoupler
with a piece of paper. Check if the corresponding
The unloading motor fails to optocoupler status shown in the screen is
Unloading motor action properly; if not, then refer to "6.10 Dismantling
overtime. and Replacing the Sample Transfer Unit" on how
to replace the optocoupler.
Unloading motor busy. 2. Refer to "6.10 Dismantling and Replacing the
Unloading
Sample Transfer Unit" to disassemble the motor
assembly The optocoupler of the
from the loading assembly and make sure motor
problem unloading motor is not
wiring is secure. If the wiring is OK, then
working properly.
The unloading assembly is one. After the installation, make the proper
not at its initial position. adjustments to the belt tension, then go to the
Unloading assembly busy. Self-test". If the assembly is working, then it has
Unloading fails.
Unloading action overtime.
7 1. The user enters "Status > Sensor" screen and

The pinch motor fails to covers the center of the pinch optocoupler with a
leave its initial position. piece of paper. Check if the corresponding
Pinch motor optocoupler status shown in the screen is
problem blocked. If it is, then the optocoupler is working
The pinch motor fails to properly; if not, then refer to "6.8 Dismantling and
return to its initial position. Replacing the Mix Assembly" on how to replace
the optocoupler.
159
Problem Solution
Name
Pinch motor action pulley is slipping. If it is, then replace the stopper
overtime. screws of the pulley (you will need to apply
thread adhesive).
3. Refer to "6.8 Dismantling and Replacing the Mix
Assembly" to disassemble the motor from the
Pinch motor busy. sampling assembly and make sure motor wiring
is secure. If the wiring is OK, then disassemble
the motor and replace it with a new one. After the
installation, make the proper adjustments to the
The optocoupler of the belt tension, then go to the assembly self-test
pinch motor is not working under "Self-test > Other Self-test". If the
properly. assembly is working, then it has been
successfully fixed.

The elevating motor fails to covers the center of the elevating optocoupler
leave its initial position. with a piece of paper. Check if the corresponding
optocoupler status shown in the screen is
The elevating motor fails to properly; if not, then refer to "6.8 Dismantling and
return to its initial position. Replacing the Mix Assembly" on how to replace
the optocoupler.
Elevating Elevating motor action
screws of the pulley (you will need to apply
motor overtime.
thread adhesive).
problem
Assembly" to disassemble the motor from the
Elevating motor problem delevating assembly and make sure motor wiring
is secure. If the wiring is OK, then disassemble
the motor and replace it with a new one. After the
installation, make the proper adjustments to the
The optocoupler of the belt tension, then go to the assembly self-test
elevating motor is not under "Self-test > Other Self-test". If the
working properly. assembly is working, then it has been
successfully fixed.

The mix motor fails to leave covers the center of the mix optocoupler with a
its initial position. piece of paper. Check if the corresponding
Mix motor The mix motor fails to return properly; if not, then refer to "6.8 Dismantling and
problem to its initial position. Replacing the Mix Assembly" on how to replace
the optocoupler.
Mix motor action overtime. screws of the pulley (you will need to apply
thread adhesive).
160
Problem Solution
Name
Assembly" to disassemble the motor from the mix
Mix motor busy. assembly and make sure motor wiring is secure.
If the wiring is OK, then disassemble the motor
and replace it with a new one. After the
The optocoupler of the installation, make the proper adjustments to the
unloading motor is not belt tension, then go to the assembly under
working properly. “Self-test > Other Self-test”. If the assembly is
working, then it has been successfully fixed.
10 1. The user enters the "status>sensor" screen of

the operation interface and covers the center of
Autoloader busy. any optocoupler with a piece of paper in
sequence. Check if the corresponding
properly; if not, then refer to 6.10 Dismantling and
Autoloader Autoloader action overtime. Replacing the Sample Transfer Unit on how to
problem replace the optocoupler.
2. Check if there is anything wrong with the cable
connections of the autoloader panel. If not,
unplug and reconnect the cables or replace them.
3. Check if there is anything wrong with the power
Autoloader did not move. supply of the autoloader panel. If not, check the
wiring of the power supply or replace the
autoloader panel.
11 1. Check if the counter stopper sheet is blocked. If it

is, refer to 6.10 Dismantling and Replacing the
Counter falsely triggered. Sample Transfer Unit for clearing.
2. Check if the wires of the counter optocoupler are
properly connected. If they are not, refer to 6.10
Dismantling and Replacing the Sample Transfer
Unit for replacing the wires.
Counter
Counter trigger abnormal. 3. The user enters "Status > Sensor" screen and
problem
covers the center of the counter optocoupler with
a piece of paper. Check if the corresponding
Abnormal counter status. properly; if not, then refer to 6.10 Dismantling and
Replacing the Sample Transfer Unit on how to
12 1. Check if the cables of the mix assembly are

properly connected. If they are not, unplug and
reconnect the cables or replace them.
Mix assembly 2. Check if the synchronous pulley is getting loose.
Mix assembly busy.
busy. If it is, refer to 6.8 Dismantling and Replacing the
Mix Assembly and replace the pulley (you will
need to apply thread adhesive to the stopper
161
Problem Solution
Name
screws).
3. Check if the synchronous belt has the proper
The mix assembly is not at tension. If it does not, tighten it up.
its initial position. 4. The user enters "Status > Sensor" screen and
covers the center of the optocoupler with a piece
of paper. Check if the corresponding optocoupler
status shown in the screen is blocked. If it is, then
the optocoupler is working properly; if not, then
refer to "6.8 Dismantling and Replacing the Mix
Assembly" on how to replace the optocoupler.
Mix action overtime. 5. Enter Self-test > Other Self-test, run the mix
assembly’s self-diagnostic, and check if the
motor is working properly. If it is not, refer to 6.8
Dismantling and Replacing the Mix Assembly and
replace the motor.

covers the center of the unloading end
optocoupler with a piece of paper. Check if the
corresponding optocoupler status shown in the
screen is blocked. If it is, then the optocoupler is
Unloading working properly; if not, then refer to "6.10
optocoupler Unloading tray is full. Dismantling and Replacing the Sample Transfer
problem Unit" on how to replace the optocoupler.
2. Check if the wires of the optocoupler at the
unloading end position are properly connected. If
they are not, refer to 6.10 Dismantling and
Replacing the Sample Transfer Unit and replace
the wires.
The CC Refer to "6.11 Replacement of the Main Control

source The CC source voltage is Panel" to replace the main control panel.
14
voltage is abnormal.
abnormal
Refer to "5.1 Integral Replacement of the Optical

Assembly" to disassemble the optical shield cover
and see if the optical laser is on with normal power
The laser input (the normal measurement range of the optical
The laser current is
15 current is power meter is 4.8+/-0.5.) If the optical laser is not
abnormal.
abnormal on or the measured optical power is far lower than
4.8, this means that the laser is burnt out. Please
refer to "5.1 Integral Replacement of the Optical
Assembly" to replace the entire optical assembly.
The 12V Abnormal +12V power. Refer to "6.11 Replacement of the Main Control
power is not Panel" to replace the main control panel.
16
working
properly Abnormal -12V power.
162
Problem Solution
Name
1. Take another measurement after performing the

soaking with probe cleanser to see if the problem
has been resolved. If not, enter into the system
for multiple times to check HGB background
voltage. If the voltage is steady and exceeds the
standard ratings, please perform the following
procedures.
Abnormal HGB background
2. Go to "Setup > Host Settings > Gain Settings"
voltage
screen, adjust the current HGB gain and set HGB
(The rated range of background voltage within 4.5+/-0.1V.
Abnormal background voltage is
17 background 3. If the problem persists, please try to clean the
4.2~4.8V; a system
voltage transmitting end and the receiving end of the
message "abnormal
HGB bracket. These two areas should not be
background voltage" will be
cleaned with alcohol or organic solvents. Instead,
shown to remind the user to
use a rubber pipette bulb for purging. If the
adjust HGB gain.)
counting bath is contaminated with overflown
liquid, wipe it clean with tissue paper that will not
flake.
4. If the problem persists after the above steps are
taken, please consider replacing any relevant
components such as the HGB bracket or analog
panel.
Check if the ambient temperature is within the

The ambient temperature specified range of 15~30°C; if yes, refer to "6.14.2
exceeds the working range. Disassembling and Replacing the Temperature
Sensor" to replace the temperature sensor.
Check if the wiring between the temperature sensor

DIFF bath temperature
and the heating plate in DIFF bath is loose. If the
exceeds the working range
connection is OK, replace the DIFF bath directly.
Abnormal
18
temperature Check if the wiring between the optical temperature
sensor and the heating rod in the DIFF bath is
loose; If the connection is OK, use a thermometer to
The temperature of the measure the temperature inside the aperture at the
optical system exceeds the top of the front-end optical assembly. Check if the
working range temperature is within the working range.
If it is not, replace the heating rod; otherwise,
replace the temperature sensor.
The positive-pressure Refer to "3.6.11 Problems with Creating Positive

chamber fails to create Pressure" for troubleshooting.
pressure
Pressure Abnormal pressure of

19 chamber positive-pressure chamber
problem (lower than normal)
Abnormal pressure of
positive-pressure chamber
(higher than normal)
163
Problem Solution
Name
The negative-pressure Refer to "3.6.12 Problems in Creating the Negative

chamber fails to create Pressure" for troubleshooting.
pressure
negative-pressure chamber
(lower than normal)
negative-pressure chamber
(higher than normal)
Flow chamber clogging. Refer to "3.6.6 Clogging of the Flow Chamber/DIFF

Clogging of Probe" to clean the flow chamber or sample probe
flow for multiple times. If the problem persists, refer to
20
chamber/sam Sample probe clogging. "6.14 Replacing the Sheath-flow Sensor and
ple probe Temperature Sensor" to replace the sheath-flow
sensor.
1. Check if the reagent has expired or is

contaminated.
2. Go to the "Service > Maintain" screen and click
cleanser soaking to clean the hydraulics. Then
return to the "Report" screen and conduct
background measurements to see if the problem
Abnormal background.
has been resolved.
Abnormal (One or multiple results of
21 3. If the problem persists, please check for any
background background measurement
peripheral interference from the grounding wire or
exceed the background
shield wire, or if any electric-brush devices such
range.)
as drills are being used on and off in the area.
This can influence the counting results.
4. If there is no such interference, please check the
airtightness of each syringe and the rear
chamber of the counting bath. If the airtightness
is unsatisfactory, then replace accordingly.
1. Go to the "Service > Maintain" screen, click

"Unclog".
2. Go to the "Self-test > Valve & Pump" screen, click
Abnormal
Valve 27 and check if it is working. If it is, refer to
22 WBC WBC clog
"3.6.4 Aperture Clogging of the WBC Channel"
counting
for operations.
3. Go to the "Service > Maintain" screen, and
perform "WBC Channel Cleanser Soak".
164
Problem Solution
Name
1. Go to the "Service > Maintain" screen, click

"Unclog".
2. Go to the "Self-test > Valve & Pump" screen, click
Abnormal Valve 27 and check if it is working. If it is, refer to
23 RBC clog
RBC counting "3.6.5 Aperture Clogging of the RBC Channel" for
operations.
3. Go to the "Service > Maintain" screen, and
perform "WBC Channel Cleanser Soak".
Check the connection of the float sensor at the rear

Waste
section of the machine; if the connection is OK,
24 container is Waste container is full.
replace the float sensor for waste overflow
full.
detection.
[DIL-A] expires. Expired reagent or insufficient residual amount.

This means that the reagent has gone out of date or
Insufficient [DIL-A]. the amount remaining is insufficient to support
[DIL-A] is not replaced. hydraulics operations such as counting. Please
following the troubleshooting procedures below:
[LYA-1] has expired. Go to the "Service > Reagent Management >
Setup" screen, scan the barcode of the new reagent
Insufficient [LYA-1].
as shown in the alarm message, and then load the
LYA-1 not replaced. reagent to resolve the problem.
Abnormal [LYA-2] has expired.
25
reagent
[LYA-2] has not been

replaced.
[LYA-3] has expired.
[LYA-3] has not been

replaced.
Check for the availability of the relevant reagent. If

the reagent is available, then check if the
optocoupler of the reagent testing panel is on. If not,
adjust the screw of the potentiometer and rotate
counterclockwise to see if the optocoupler can be
26 No reagent DIL-A running out. activated.
 If it works, drain the liquid from the tube and
use a multimeter to press the spot as shown
in the picture. Adjust the potentiometer and
set the voltage at 1.5±0.05V (the testing
points are all located in the left part of the
165
Problem Solution
Name
picture, among which Potentiometer A
corresponds to Testing Point A, and so on
and so forth).
LYA-1 running out or air

bubbles in inlet tubing.


 If it is not working, refer to "6.13 Replacement

of Reagent Testing Panel" to replace the
reagent testing panel.
Check if the right-side door is open. The door

should not press against the microswitch. If the
Right side door is open.
door is properly closed, please replace the
Door or cover microswitch on the side door.
27
open error
Open the optical shield cover, then close it again to
Optical assembly cover is
check if the problem has been resolved; if not,
open.
replace the optical microswitch.
9.2 Trouble Analysis and Solution on Network
166
Connections
The following refers specifically to trouble analysis and error identification regarding failures to
connect the analyzer operating software to the main unit
1. Confirming the power indicator light on the back panel
The 5 power indicator lights on the top right corner should always be lit and red in color once the
machine has been powered on, as shown in the following picture.
2. Confirming the status of the main control panel after power on

Multiple green status indicator lights on the main control panel should be on after the machine
has been powered on.
3. Confirming the status of the dial-up switch of the main control panel
Please confirm that the dial-up switch is in the "OFF" position as shown in the following picture.
4. Confirming the status of the network card indicator light

Confirm that the PC network card indicator light is green and blinking.
5. Confirm that the status of the main unit network card indicator light is green and blinking, as
shown in the following picture.
167
6. Confirming IP Settings
Enter the WINDOWS Network Connection Settings screen as shown in the following picture.
Confirm that the IP address settings are correct as shown in the following picture.
7. Confirming the network status test

a. Press [Win+R] key to enter the Run window.
b. Run the ping 10.0.0.101 command.
168
c. Check the running result of the ping command.

There is nothing wrong with the network connection as indicated in the following picture.
There is something wrong with the network connection as indicated in the following picture.
It may be caused by abnormalities along the network cable. Please try again after replacing
the network cable.
169
Appendix A Maintenance Inventory
A.1 PCBA List

No. Material No. Material Name
1 22010013A PCBA of the main control panel
2 22010014A PCBA of the driver panel
3 22010002A PCBA of the rear panel
4 20010024A PCBA of the Reagent testing assembly
5 22010017A PCBA of the key panel
6 22010018A PCBA of the indicator light panel
7 22010015A PCBA of the autoloader panel
A.2 List of Cables and Wires

1 37010010A Control wire for AD input
2 37010013A Grounding cable for the outlet
3 37010014A Power cord for silicon photodiode
4 37010016A Power supply wire for switch 1
5 37010017A Power supply wire for switch 2
6 37010019A Optical grounding cable
7 37010020A Power supply wire for the outlet
8 37010021A Grounding wire for the rear panel
9 37020003A Control wire for the mixer
Control wire for the stepping motor of the sampling/sample

10
37020005A syringe
Control wire for the stepping motor of the lyse/diluent

11
37020006A syringe
Control wire for the stepping motor of the sheath flow

12
37020007A syringe
170
13 37020011A Control wire for the syringe optocoupler
14 37020013A Control wire for the horizontal optocoupler
15 37020016A Control wire for the temperature sensor
16 37020024A Control wire for the housing panel
17 37020025A Control wire for the laser
18 37020026A Control wire for the optical system
19 37020027A Signal wire for silicon photodiode
20 37020028A Reagent control wires
21 37020030A Network cable
22 37010026A 1105 Valve control wire
23 37010027A 1105 Control wire for heating
24 37010028A 1105 Valve control wire 1
25 37010029A 1105 Valve control wire 2
26 37010030A Optocoupler wire for the loading motor
27 37010031A Optocoupler wire for the unloading motor
28 37010032A Optocoupler wire for the lifting motor
29 37010033B Optocoupler wire for the swing motor
30 37010034A Control wire for the loading motor
31 37010035A Control wire for the unloading motor
32 37010037A Wire for the feeding micro switch
33 37010038A Power cable for the autoloader
34 37010039A Connection wire for the can
35 37010042A Control wire for the sampling motor
36 37010043A 1105 upgrade wire
37 37010044A Control wire for 1105 key panel
38 37020050A Control wire for the float sensor (1105)
39 37020051A Control wire for the sampling optocoupler (1105)
40 37020052A Control wire 2 for the sampling optocoupler (1105)
41 23990012A Wire for the gas pump
42 37010002A Power Cable
171
A.3 List of Wearing Parts

1 23990007A Wire for the location optocoupler
2 23990016A Wire for the temperature sensor
3 23990027A Outsourcing wire for 1105L optocoupler
4 23990028A Outsourcing wire for slow-down stepping motor
5 23990029A Outsourcing wire for 1105 side-out optocoupler
6 23990030A Wire for the loading microswitch
7 23990037A Outsourcing wire for 1105 aspiration micro switch
8 50990006A Built-in Barcode Scanner
9 53990036A Inline filter
10 60010027A Aspiration touch panel (black)
11 56010280A sampling needle (Piercing-Needle)
12 56010408A 1105- Cleansing swab
13 23990004A Wire for the 3-way electromagnetic valve (S)
14 23990002A Wire for the 3-way electromagnetic valve (L)
15 23990003A Wire for the 2-way electromagnetic valve (S)
16 23990001A Wire for the 2-way electromagnetic valve (L)
17 23990005A Wire for the pinch valve
18 24130009A One-way valve
19 23990008A Wire for the liquid pump
20 24050008A Synchronous belt (MXL) TBN72MXL025
25 24010002A Air pump
26 53990018A Air filters
27 24020005A Stepping motor
172
28 60100011A DH71 blood cell screen-printing housing
31 60100023A A1105OEM screen printing on the housing panel
32 60100028A B1105 screen printed housing panel
A.4 List of Consumable Parts

1 63010023A Double-layered tube for maintenance
2 63010024A EVA tube for maintenance
3 63010025A Slim No. 50 tube for maintenance
4 63010026A Wide No. 50 tube for maintenance
5 63010027A No. 3603 tube for maintenance
6 63010028A MPF tube for maintenance
7 63010029A 1.5mm Teflon tube (1100mm) for maintenance
8 63010030A 1.0mm Teflon tube for maintenance
9 63010031A 2.4mm TPU rubber tube for maintenance
10 63010032A 0.78mm silicone tube for maintenance
11 63010033A Pharmed tube for maintenance
12 63010034A 1.6mm thin silicone tube for maintenance
13 63010035A 1.6mm thick silicone tube for maintenance
14 63010036A 3.2mm silicone tube for maintenance
15 63010037A 2.0mm (i.d.) Teflon tube for maintenance
16 53050003A T420-1 connector
17 53050005A K420-6005 screw-thread connector
18 53050008A S220-6005 screw-thread connector
19 53050010A L420-1 connector
20 53050011A Y230-1 connector
21 53050012A Y420-1 connector
173
22 53050013A N430/420-1 connector
23 53050014A N420-1 Barrel connector
24 53050016A MTLL230-1one-way flow-adjusting connector
25 53050044A MTLL013-3 male Luer lock ring
174
A.5 List of Parts and Assemblies

1 20010040A 2.5ml syringe assembly made by Dymind

2 20010041A 10ml syringe assembly
3 20010042A Sheath flow syringe assembly
4 20010043A μl syringe assembly
5 20010007A WBC counting chamber assembly
6 20010008A RBC counting chamber assembly
7 60010012A Isolation chamber
8 20010054A Positive-pressure chamber assembly
9 20010055A Negative-pressure chamber assembly
10 20010018A DIFF bath assembly
11 20010019A Optical assembly
12 53200001A Towing-chain connectors
13 53990001A Towing chain
14 23990020A Belt pulley
15 56010004A 15MXL driven wheel
16 20010002A Power assembly
17 20010075A Mix assembly
18 20010078A Loading assembly for the autoloader
19 20010079A Feeding assembly for the autoloader
20 53990041A Linear sliding track MBC09SN-1-L-155-N-Z0
175

S-65.02.0018A (4.0) DH76 Auto Hematology Analyzer Service Manual

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Auto Hematology Analyzer

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Contents ............................................................................................................................................ iii

3.6.5 Aperture Clogging of the RBC Channel ......................................................................... 39

6.2.1 Open the Left Side Door ................................................................................................ 94

8 Comprehensive Device Debug ................................................................................................. 145

1.1 Who Should Read This Manual

1.2 Symbols and Legends

The operator should follow the instruction below each symbol to

The operator should follow the instructions below each symbol to

The operator should follow the instructions below each symbol to

When you see It means

Exercise caution to prevent puncture

Laser radiation warning:

Instruction for Moving

Alternating current (AC)

For in vitro diagnosis only

When you see It means

The device is in full compliance with the council directive concerning

Authorized Representative in the European Community

Humidity level for storage

Atmospheric pressure level for storage

Consult the operator’s manual

Let this side face upward.

When you see It means

Fragile, handle with care

The analyzer, after being scrapped, should not be disposed with

1.3 Safety Instructions

2.1 Mechanical Components

Figure 2-2 Back view of the analyzer

1 - Diluent inlet 2 - LYA-1 lyse inlet

3 - LYA-2 lyse inlet 4 - LYA-3 lyse inlet

5 - BNC socket for the diluent sensor 6 - Power input socket

7 - Waste outlet 8 - BNC socket for the waste sensor

9 - LAN network interface

Figure 2-3 Front view of the analyzer (housing removed)

1 - Sheath flow syringe assembly 2 - Small valve assembly

3 - Sample probe assembly 4 - Mix assembly

5- Sample Transfer Unit 3

1 - DIFF reaction bath assembly 2 - Impedance-counting reaction bath

3 - Filter 4 - Negative-pressure pump assembly

5 - Negative-pressure chamber 6 - Large valve assembly

7 - Positive-pressure chamber 8 - Positive-pressure pump

9 - Optical assembly 10 - Autoloader Panel Unit

11 - Mix assembly 12 - Sample probe assembly

Figure 2-5 Left-side view of the analyzer (Left-side door removed)

1 - μl syringe assembly 2 - Diluent syringe assembly

3 - Lyse syringe assembly 4 - Liquid-level detecting assembly

5 - Power switch assembly 6 - Driver panel assembly

7 - Main control panel assembly

2.2 Hydraulics System

2.3 Hardware System

2.4 Software System

3.2 Principle of the Hydraulic System

DIFF WBC/HGB RBC/PLT

module module module

LYA-3 LYA-2 LYA-1

3.3 Hydraulics Diagram for the Overall Unit

3.4 Time Sequence Description

3.4.1 DIFF Measurement Channel