LC Technical Note

99 Analysis of Valsartan
GL Sciences Inc.

Valsartan is mainly used for treatment of high blood pressure. Valsartan dilates blood vessels and reduces blood
pressure because it acts as an antagnoist of angiotensin II receptor.
I this
In hi note, chromatograms
h off valsartan
l b d on the
based h condition
di i ddescribed
ib d iin the
h UUnited
i d States
S Pharmacopeia
Ph i (USP)
are shown. Using InertSustain C18, sharp peak was obtained from tablet samples as well as standard solution.

Standard solution Tablet sample

UV 225 nm 1. Valsartan (500 mg/L) UV 225 nm

The sample concentration was
200 400 600 800 100012001400
0

200 400 600 800 100012001400

0
adjusted to 400 mg/L as Valsartan.
1
1
mAU

mAU
2

2
0

0
0 2 4 0 2 4
Time (min) Time (min)
UV 273 nm UV 273 nm

1
1
200

200
mAU
U

U
mAU
100

100
0

0 2 4 0 2 4
Time (min) Time (min)

Conditions A sample pretreatment

Column : InertSustain C18 HP (3μm, 50 x 4.6 mm I.D.)
Column Cat. No. : 5020-14442 method for tablet sample
Eluent : A) CH3CN
: B) CH3COOH
Sample
: C) H2O Grind tablet into powder
A/B/C = 500/1/500, v/v/v Weigh the powder equivalent to
Flow rate : 1.0 mL/min 80 mg of valsartan.
valsartan
Column Temp. : 25 ℃
Detection : UV 225 nm and 273 nm Extraction
Injection Volume : 5 μL
Add mobile phase
Chemical structure Sonication extraction for 10 min
O Make up to 100 mL with mobile phase
H

N
COOH
N N Filtration
HN N
0.45 μm-membrane filter

Dilution
Structures are created using Chemistry 4-D Draw which is
2-fold dilution with mobile phase
provided by ChemInnovation Software, Inc.

HPLC
GL Sciences,
Sciences Inc.
Inc Japan
22-1 Nishishinjuku 6-chome, Shinjuku-ku, Tokyo, 163-1130 Japan
TEL: +81-3 (5323)6620 FAX: +81-3 (5323)6621
http://www.glsciences.com/products/contact.html
E-MAIL: world@gls.co.jp