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VP-03 Electronics Gain Adjustment Version - 202667-102_3852_7

Inspect tools for damage, ensure calibration is not expired and replace if necessary.

At the completion of this VP: G110 - After repair is complete, verify per released Operation and Service Procedures. If the system/instrument
produces results, ENSURE appropriate Quality Control is in specification and calibrate as necessary.

VP-03 Electronics Gain Adjustment

Purpose Module CELL-DYN Emerald, CELL-DYN

To adjust the WBC and RBC/PLT gains and verify the HGB gain. Emerald 22 and CELL-DYN Emerald
Perform this procedure: 22 AL

After replacing Counting Chamber or Counting Head

CPU
Preamp

Materials Time 30 min.

Required Standard Reference Particles (SRP):
8160601101 MICROSPHR POLYMER 5.0 DIA EMLD

8160601201 MICROSPHR POLYMER 3.0 DIA EMLD

8160601301 MICROSPHR POLYMER 7.0 DIA EMLD

Note: An RBC/PLT SRP mixture of 6 drops of 5µm +4 drops of
3µm spheres is used in the Gain Adjustment process.

WARNING
Potential Biohazard

Note: The Standard Reference Particles (SRP) part numbers listed above are specific for use on the CELL-DYN Emerald, CELL-
DYN Emerald 22 and CELL-DYN Emerald 22 AL.

Note: Instructions are for software version 2.1.0 and above for Emerald, version 3.0.0 and above for Emerald 22, and version
1.1.0 and above for Emerald 22 AL.

Action Steps Reference

Preparation
1. Logon. Use the Field Service
password: AFSE.
2. For the Emerald and Emerald 22:
From the MAIN MENU, click
[MAINTENANCE]
[ABBOTT SERVICE]
[SET GAINS].

3. For the Emerald 22 AL: From the

MAIN MENU, click
[MAINTENANCE]
[ABBOTT SERVICE]
[OPTICAL/GAIN ADJUST].

WBC Gain Emerald Screen

Adjustment 1.Verify AUTO box is checked on
CELL-DYN Emerald instrument. On
Emerald 22 and Emerald 22 AL,
adjustment is always AUTO.
2. Verify bead size is correct. If not,
enter correct value from bottle label in
the DIAMETER field [1].
3. Shake the 7.0µm microspheres with a
vortex system.
4. Aspirate microspheres on the
Emerald or Emerald 22 instruments:

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a. Place the well-mixed 7µm
undiluted bead solution under
the sample probe.
b. Click [WBC GAIN].

5. Aspirate microspheres on the

Emerald 22 AL Instrument.

a. Put 10 drops of the 7.0µm

microspheres into a new no
EDTA sample tube.
b. Place the open tube of 7.0µm
microspheres into the Open
Tube Holder for aspiration..
c. Click [LATEX WBC]).

6. At the end of the cycle, verify the Emerald 22 Screen

channel recovery value is within ± 2
channels from the target channel [2],
and the WBC GAIN is within 210 -
250 [3] for CD-Emerald, the WBC
GAIN is within and within 166 - 220
[3] for CELL-DYN Emerald 22 and
the LATEX WBC is within 166 - 220
[3] for CELL-DYN Emerald 22 AL.

7. Continue with Action: RBC/PLT

Gain Adjustment or proceed to
Action: Verification.

Emerald AL 22 Screen

RBC/PLT Emerald Screen

Gain 1.Verify AUTO box is checked on
Adjustment CELL-DYN Emerald instrument. On
Emerald 22 and Emerald 22 AL,
adjustment is always AUTO.
2. Verify bead size is correct, if not-enter
correct sizes from bottle label in the
DIAMETER field [1].
3. Prepare the RBC/PLT bead mixture
by combining 6 drops of 5µm beads
with 4 drops of 3µm beads in a tube.
4. Shake the RBC/PLT mixture with a
vortex system.
5. Aspirate microspheres on the
Emerald or Emerald 22 instruments:

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a. Place the well-mixed 3µm and
5µm microspheres bead
solution under the sample
probe.
b. Click [RBC PLT GAIN].

6. Aspirate microspheres on the

Emerald 22 instruments.

a. Place the well-mixed 3µm and

5µm microspheres bead
solution into a new, no EDTA,
sample tube.
b. Place the open tube of the
well-mixed 3µm and 5µm
microspheres bead solution
into the Open tube Holder for
aspiration.. Emerald 22 Screen
c. Click [LATEX WBC]).

7. At the end of the cycle, verify the

channel recovery value is within ± 2
channels from the target channel,
and following [2]:

8. Continue with Action: HGB Gain

Adjust, or proceed to Action:
Verification.

Emerald Emerald
Instrument Emerald
22 22 AL
RBC Gain
target range 210-250 160-220 160-220
[2]
PLT Gain
target range 210-250 160-220 160-220
[2]

9. Continue with Action: HGB Gain

Adjust, or proceed to Action: Emerald AL 22 Screen
Verification.

HGB Gain
Adjustment 1. Click [ADJUST HGB] icon.
2. At the end of the Cycle, verify that the
HGB BLANK gain value is between
150-254

Note
There is no HGB target
channel adjustment.

3. Continue with Actions: WBC Gain

Adjust, RBC/PLT Gain Adjust, or
Proceed to Action: Verification.
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Verification
1. Click [CONFIRM].
2. Click YES when the WARNING
window appears, to save the
adjustments.

Note
To exit the screen without
changing the value(s), click
[ESC].

3. Log in as Operator and run

background.
4. Verify calibration by running controls
and calibrate, if necessary.

CELL-DYN Emerald Service and Support Manual (Version 202661-107) • © 2007, 2018 • Abbott Laboratories, Abbott Park, Illinois • All rights reserved. • CELL-DYN and
CELL-DYN Emerald are trademarks of Abbott Laboratories in various jurisdictions.

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