DATA SIMULASI ST-1

1. Buatlah rancangan jumlah komponen dapar yg digunakan (dapar sitrat) utk

membuat dapar pH=3,5 , dapar pH=5,0 dan dapar pH=6,0 masing2 sebanyak 200 ml,
dengan kapasitas dapar =0,01. Bila diketahui pKa1=3,13, pKa2=4,76, pKa3=6,4.
Diketahui : BM Na2HSitrat.1,5H2O=263,11
BM NaH2Sitrat=214,11
BM As.Sitrat monohidrat = 210,14
BM Na3Sitrat.2H2O = 294,10
(Gunakan pers. Handerson-Hasselbach)

2. Tentukan komponen dapar manakah yg akan anda gunakan sebagai adjuster apabila
ada penurunan/peningkatan pH (sesuai kasus table yg anda kerjakan)

3. Apabila dibuat dapar pH=3,5 pH=5,0 dan pH=6,0 sebanyak 1L (pH dapar spt tabel),
ketika .diambil sampel 200 ml, perlu X ml adjuster.(data soal). Maka .untuk 1 liter
dapar berapakah jumlah (gram) adjuster yang ditambahkan  ?  Apakah kapasitas dapar
tetap ?

Tipe 4
pH pH terbaca pada pH meter Schott Conc Adjuster Vol adjuster yg ditambahkan
teoritis (dalam 20 ml hingga tercapai pH teoritis
3,5 4,72 15% 5,5 ml
5,0 6,55 15% 6,5 ml
6,0 5,20 15% 7,5 ml
3,5 4,87 15% 8,8 ml
5,0 6,85 15% 9 ml

Jawab :

1. Diketahui :
pH = 3,5 ; pH = 5,0 ; pH = 6,0
Volume = 200ml
Kapasitas dapar= 0,01
pKa1 = 3,13 ; pKa2 = 4,76 ; pKa3 = 6,4
BM Na2HSitrat.1,5H2O = 263,11
BM NaH2Sitrat = 214,11
BM As.Sitrat monohidrat = 210,14
BM Na3Sitrat.2H2O = 294,10

Ditanya : perhitungan dapar menggunakan pers Handerson-Hasselbach ?

Penyelesaian :
a. pH = 3,5
 pH = pKa + log [G]/[A]
3,5 = 3,13 + log [G]/[A]
0,37 = log [G]/[A]
 [G]/[A] = antilog 0,37
[G]/[A] = 2,3442
[G] = 2,3442 [A]

 pH = -log (H3O+)
3,5 = -log (H3O+)
(H3O+) = antilog (-3,5)
(H3O+) = 3,6123x10-4

 pKa = -log (Ka)

3,13 = -log (Ka)
(Ka) = antilog (-3,13)
(Ka) = 7,4131x10-4

Β = 2,3 x C x Ka ¿¿

( 7,4131 x 10−4 ) (3,6123 x 10−4 )

0,01 = 2,3 x C x 2
( 7,4131 x 10−4 +3,6123 x 10−4 )
C = 0,0207 M

 [A] + [G] = C
[A] + 2,3442 [A] = 0,0207 M
3,442 [A] = 0,0207 M
[A] = 6,0139x10-3 M

 [G] = 2,3442 [A]

[G] = 2,3442 . (6,0139x10-3 M)
[G] = 0,0141

[A] = H3 Sitrat [G] = NaH2 Sitrat

[A] = 6,0139x10-3 M [G] = 0,0141 M
mmol mmol
= 6,0139x10-3 x Mr (210,14) = 0,0141 x Mr(214,11)
ml ml
mg mg
= 1,2637 x 200 = 3,0189 x 200
ml ml
mg mg
= 252,74 = 603,78
200 ml 200 ml

b. pH = 5
 pH = pKa + log [G] / [A]
5 = 4,76 + log [G] / [A]
0,24 = log [G] / [A]
Antilog ( 0,24 ) = [G] / [A]
1,7378 = [G] / [A]
[G] = 1,7378 [A]
 pH = -log (H3O+)
-log [H3O+] = 5,0
 log [H3O+] = -5,0
[H3O+] = antilog (-5,0)
[H3O+] = 1 × 10-5

 pKa = -log (Ka)

4,76 = -log (Ka)
(Ka) = antilog (-4,76)
(Ka) = 1,7378 × 10-5

Β = 2,3 x C x Ka ¿¿

( 7,7378 x 10−4 ) (1 x 10−5)

0,01 = 2,3 x C x 2
( 7,7378 x 10−4 + 1 x 10−5 )
C = 0,0187 M

 [A] + [G] = C
[A] + 1,7378 [A] = 0.0187 M
2,7378[A] = 0.0187 M
[A] = 6,8412 × 10-3

 [G] = 1,7378[A]
[G] = 1,7378 (6,8412×10-3)
[G] = 0,0119 M

NaH2Sitrat = [A] Na2Hsitrat.1,5H2O = [G]

= 6,8412 × 10-3 M . 200 ml = 0,0119 M × 200 ml
= 1,3682 mmol × Mr = 214,11 = 2,38 mmol × Mr = 263,11
= 292,9539 mg / 200 ml = 626,2018 mg / 200 ml

c. pH = 6
 ph = pKa + log = [(G)/(A)]
6,0 = 6,4 + log = [(G)/(A)]
6,0 – 6,4 = log = [(G)/(A)]
Antilog (-0,4) = [(G)/(A)]
0,39810 = [(G)/(A)]
[G] = 0,39810 [A]