anikera md ico

The eove) ne wayhed nd obie

CUY CÄM at Soc.The doied Jeoth tera J
frd nto PoLkS wih a y kine psic Sine.

Tha powo/end saed n e exbratid a Soxhlet

enksates u metkonal u ve day) ad e

awaten
odovck ohtained eNapootad uig
ka6oC The u d e etorutt oltaine4
uxd phepoona ARKaloid), flavamaid tah rin
AAKalaids -
Hc d duiny peument wo) lem

ttam m, hut rok ueakun kam 0:]m.

) 19 d mekkanal exkhat Padutikiened ehied

l00mo Chlogokonn amd O:lm Hd solukion bn a
Sepesatogy funne Two on -homo9 enLais dhe

ehtained amd t kentkiomwas baitie wit

n
whilr P komed
ppk omed
Gtmonua
Omonia Seluknon amd a P

wa indilatve ok & phen e d- alkal-

Seukion
koym baxc Selutncn
oid Aiion Choro
ek uhich ki louen
30U Ced two i c e ay
 Chganic Coponns CAllalaid) 1

The Chlonolom loypn wa Sepaatad bm

miuhe disad obl w yd a alvale

aut kr K Cnpaient.
tiglad
o'
) Flavovoidh
They ou dekunmindb eigig
Cud echtk, a akn md e r a c wi

SoCd 00. mekano akToom kempera Ay

9
ahauut had The Sahom wa tened ung
kihe &i U t w a ev
a kile Pap
wako hatt
doymp oen a

C)Tanni-
Thox de'dtoungd exkratig
Powdrd GN wiko Acetonsaand kelt
hou) allo rath milu wa Alteaå

wo Comdenyd wilk a
aby evapr
and Subsuembly died
0htaun Tannu oUhom
method 2

mIL wayhed, douedm

hotnd t a Powd en eumo
lod C,amd
CJeM af
drPowdSud Sayrple was Soaked n
Abt 0 Kem am!
m O200 M Volwmehne
99. etkanok
atlotwed Stand b 48h at oom teny tem
wa) &iltenud amd CXa Sodn
tA Soaked Samph (

wad moedy rolay eNaposalo

korcd im aVaccm
aVaccm
neNice awa
Tre dried
deicCalo oleikva
T e m r a l i a a j u n a

m a y

w a n g i a indula

mekkod 3 Collectid
u benK) wehe
ML
mo,TA amd
amd dued in Shode
Wa &kem in Sooml twaoH
S9 odied onk petuole
m o md hellon am and nekued Au3.

t/TheSduton wa) ala wed ttanmd se smakime

amd ten Ailkoud.
iu) The Vcume
Nolume o bkoate Setion wa rantalmd o l
Thei
d a ock sAuhion d tnhiloys
l00mwnth
lo0mA, hth wa)

A ,dok -t e d qsh wa died and

eiohael taken ditet on ky eypsmat

 Munayo Koengni
weho ka Ken
ThCuSy eane
Syrall pieces QUnd Thy whe died
Q
8oC
Fom u lo9 a Sanal
well to powdeh
9euxe in lo0ml- disitld wakn k ! ha
was n slthed aS,
The neltad Seluhion
amd bitbala Vdume wa mad yo loam
d i h i d w a l i n ' The anc.ddr tuck
uxy douyle
m Um 4 y (Va).
Sduhion iy
epnexd

nekkod 2
Aih-dued amd powdeed laue) o m. Koegini
(019) uhd r ehatkio twetkyl aceak
at moem np C12 haun). otmnt
EkOA)(3 Lt)
wa) uxd dihecly wikaut ptuitilatim to rdessta
extatt. Al
-hom ne Compayition ot K
oy eNa-
haLM, Et0Ae wa hemded uing a

Paa amd Ke Nemainung hesioe wa daied

SCheend Gxon nhibikiom pakenhja
 Pomdias pulds
The eak aksuios Was asn obued at 40c

m and milled into a kine Powdeh

m Om
wa extractid uilk
20 wv
nhoumd amateria
Tka T-20
uath (1:) wirg l a
TusHaX
EthOnal oncentretad wa)
model Dos 20 mmThe erbatk ar 4oc
Vacum ng a akony evapoet
Vmden ote
at -e br
mdi netigenation
amd Skoe2

w
methcd thoraughly woshed
The fheh leaves wehe tkem a Kruiks
peel Sead e
3emae Sail a dint while a
eoued and Cho ppes s
wR wRh Temoeed BoK
sehet n shode duicd Ar 2 weeKs sullbwed 9tndiny
wehe eKept n kue Sepeat
martne poukile)They
atoom kemp. mhil kuuA l d .
Closed Containesu
Extoaion mateuals uene Soaled in
Th pudveuzed plant
ContaineM and kept drd
3.SL o metkono in Sepasout
week itk occoasional sháxiny The uhole mixttten re

caton ollaw ed
Then lkead ooagk Stenilned

amd ConCenbalad
uhabman popeh
No1 lken
evaposalos The weight o
g a 9atovy
 exsact huib amd auve
Cude
A kotal o
09 Chude
l01, epeckively
13 clath pkam matias wa Kem PostA
exlhatt
amd. dichlaomelio
oned witK'Chlanokeat

pehcemtageields aleales
ostsruik
The
methanal (21:3., Is chloso
CKkaolrsm (19.5y. 231
(2o.9 2S:44) he)peckiey
amd dichlvometkone

melkad
skem onk d S. dulcis ue
h e
washed wtk wale7 immediatel ako, CoVakien
tpan k uay Choppeslnto Sall piecen,ai dhuee
9
at oom kemp: aheut lo day amd ksne!
nla potden K9)uth. uwa insuxd im 6L 4p
etkanol lor day o kempi2Tets), Aktth
day, ea extau AteradLough Gatbn
Calon plug
pla

and Kim agh whomom nd.1 Pitke papeh

The ebatk wa), Concembatid mden eduCed he
e belaus Socl auh Tokoy Vacuum wape
Comc.'eytaut w a s t t d ar Hc
ab.The
 method
hude
) The leaue
wehe Sepenaid sgm Stem acleamed
Paortis- amy mpusiktes
with a CRan U . The tan
d
Suhiectid o ais chying neatmemt at room ktmp.
wen
wa)
i Uhu Shade Umkil
a Conytant eight aChuied
23 1) )Th doed keaueN n e em 9hotnd nto ksine potde
a laboraly endeh amd stoved o c er
wng
rents amalyi
3Eoattion wan done y metkod dmedianieral.

K plant Sample wa)

ighed amd Soaked in
a to9 d
Ueckicn T elkoonol amd Suhje ckd ko Sonitation
dhied lo CmL o 10
ktmp: 26-4dc) wg a tkemo
kusne!
aa a Covtlld
CJea Jur I a s
L pure100 Ulho Semic
Wa)
wwatnam
1 Astn 7 The mi lde
hude extac Was
amd
iUo papen no eNaporako.
u g a pky
fapeh Ve CCum-euapora led hed e d
huite ug
VCed he)-Tke ocewa hepeataed
to achiene maXyiell Te Chuclk exhatk
m waper- ation
a San vGc
a d e z e -dsled
n
wehe ueighed
Cool Salke Phene dyh tm
4)Preos dsuea Sample wesra cd a Ye
mwhk kokn amalyin