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ees Dd) Descbe wetesh lo

hs@)
Sa)WeMerm b Alia - * e eserh l o t Sol-efines Colled

h e pofeln tnnuhobla or werlerh sloA


a wide dahalhical-eahaue n Vnecular

Diogy ond imuno genedS Ao defet pecic


phoAein ih a aple doPne homogenahe

She
SO

37

weslem Llt ugi a anlibocy hak


Pecs ghle o e wodiled wh pore cid

In barefthe Saple ndergoe Pd»leln dehauehon,


golowed by gel eleefvo phoholh
Spnthelie or anlnal- deived andi bodly CKhowh as
Ahe phmar andi' bod, ) i4, Oeated hat Ke Coghiges
9nd binds do a
Specihi faget ppotein. he
ele ctbopho desi eh b)ane 14 washed in Solutio
Cohfein Ihg he
pmay aAb edby ,bodve exc8 Ghbr
19 wane d
A Seconda ad'body 1 added shi'eh Raojnis ea
Ctud bihds do the piman ondi bod Secohdety
auibody t'$ ViGuaieed Hro uyh va bios, medods
Suoh as Seni
houod ecehee. Qhd Radio aclhhy, allosin
indibect deleeio oAe peciJr asgek odem

he a e wolerh blot i, pay on h e Souhen


c

blet a echnigue for DNA defe cdion valned


te
Hs mvenlor Lnbish bolosist Edwin Southerh.
SiUoyy
detecdou
NA is Aced as hoymer blot Ac
e x werlem Wo
Cwa gineh by w. Neal Burhel
B8,aAhouyn Ahe wmcthed
Hsel otginakcd
h 1973n Jaloytery hay ow bin athe
iedic h Miecher insiLde
B Between 1S79
in Bahel.SHzeslaud.
and 9o2b ha been
h he enhioncdd
dtle,ob eaveol Cnd
key woyds
ove han
4oo,o0 R.L Med sfeo
Qud weyt be the t Pollcaous"
ugcd ppod ein -airnayJtal
fechne
Soulhe lo
BOot+ bioly)
ERectroLo-Hy
uey b
the t soeten DNA was done n 1865
Fiedseh Miedher CuyrenAG, t i cRoudte
ooce dune n dlecuNar bioleqy or deren Rlc akoge
Por the chece Melhod, fhere and Seleelhny the
Coect
ctoe w ane e oh Kr ophml2aHon
aud Cfcdton 0Cedaes PCSeuBrliidy deleeho
ConGidehed fo shos e aiado bedween
eConme FCial H.
Baaie rcedure -

Cols wdh ane o be Sfudte e eeed to be


Colle Ched
opon fo epege dhe
Bean dhe CeMnebra ue
ONA alhg wi e Cyebopkasun tuhh (ce reis)

Lipidsonhe Ce e orethe and he het clez


ane b k e down wih dederqeuds od Seidachans.

Brea y o w ofein Adding a rofease

(Peievl)
EeaPr dou PNA by adting ah KNase (Cprul)

h e SAion i4, ealed ith ohcen-baled


Selt Solbeio (Saline) fo ake b dobit, c h a
baken poteus, dhds uA lumt
and teyedree,
Coutetfyecte t n e SoQarto wuth Sepyetes dhe
claped Cellnevr cdebis, dron the DyA
DNA Pihcaon o dderget Proheins Sg qncd
Reagenth ued luinhe cell Dy4 Skb he
Lest Cow uged poCednes
A sato he NA s died,oved 1n q
sRighy alkaline b-fler, usally in a
TE bu Ffe ov h e puve e

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