551NMENT

CHoph& Heme
Prolais
Subntted to
| Mrs. AkshataMaam
biochemiskpy lalam
Subnitted b.
OLy Maiumdei.
Yeoo b.Se. Nwetng

Subwited Cn: 1/07/2024

 Chlorephul
Chloroph a pmoteinacaeus piqmer
involed in- photosurdhesis,
ocu3 in 3eseal distinet dooms
Chloropul
-chlorophyla 7 major
- chlorophuyll b
p e ebound. in kiowr plantz 4 green alae, chloephps
e d ase tound, oten cotha, in diPacert algae
choncphes a nae pe tsund In 30sre golder
Oga.
Baelbuo-chlorophull oevg in eodain baclixia
ceeug in membrangus
green plang, chlorophyel
ealleol-
disklika unit eallod hypakoids, maqanelke
anleus.
chloropla gises plants heir green

Sructure_Chlorophy
a t e Soluble eklonophu proteing (Wsep) ove
hornotdpameie poteims hat bind douve (4) hlonophyl!
(ch) molkeules in iderdieal bindingg siBes, cohich rok
WSefs acod moddl do sludy preten-piqmont irracekion
ho ehlorophll nolocula onsist ot a
centnal mognosiurm otorn(Ha2) Auveundad baa
nieoqen ertaininq uotioa ealic a - PoPphypin ina
D
attachocto the in isa lona) Rorbon-hugbogen sidai
chain. knoSN as a phyol ehain.
 ceuronce Mot nahoaly oeuvoung qpecm eqdahles
econtatn- eklonophu.
rody 3ich in eWonophy! potiro.ecouus udsbae
include
Spinach brocal
Colad greens Gueer epbooey
Mautand gneens
Chlorella 7yposou
G9een beons pe
Spiuulina
AValta Mateha (grear 190
Paslo
besides ehlorophal Ahose ventalas preride a
vadepeb hoalthsul vidomins minesol,.

enifts
aditionally, hlomephugl*
a health supplement in medieiv:
sce nd as

cserld. ChlorophUl ean helybodl do

be
-
tcotet oe Skin dicac
mino lkor:s
(denalilis)
-body domes
- dighlimy' eotain ini, ttes
 Some ob the peterdial centbtl ob chdorophll
inelude :-
dnti-agina Kemec- spphgina?
d
a qel eontaining
chloraphaylin to he al
tduces iqrs, ot photoogimaagir? dusa o
AUm expouvo)
exeeuiw

Aene Jreatment ppena gel eriainin

Chlonophylin holps to
secuuee hacial aene& Jaqe vigi bla pores.
Body Buildina) fnoperies Suggestd b
D D
Kesearehoy hot
Goheatgneas use hich ia sieh in ekloroph
holpded an tneakirg hemoglobin desieiencp dösoodso,
SAAch o3
anaeioa f thalassemia

Wound- healino) froperies MediaionContani

eset un
ehlorophu provoi
healing) and 9cccluuees
odorg. (Somo doetbna
ppoerilbe tij medicakin taday).
Caneer Jreatmert Chlorophyl kas shseor
pokentio as a
eaneo
Aaeatmertt in seme eorduetd in animal,
Caes or
proro to eases ike eo lor eamcan
ne caMeRLuko diease,.
 Ctho possible haallh benidit îmelude chlonopl
eseets on
nereased entocoy
Homonal alonce
A-lhoilt, dibromujalgio solie
Leight oss.

Chlorophullas ofroeh
ho ma coeniend mathod ot epoarstng
ho9ewlatfve values i as qptiadensihp (OD),or (D) per
o t sup cocioht peu ml. et solution "cm. absopior

JLe percorlage c ehloroph 1 is D (900)

(too)/5.Gnd
o 1.61 D

Aguous ebaet ot Spinoch 4 spidisra

eanes ied highl opaloscomt poeparations shich
Ooce not in rue 30lution.

2. he dillenences beluoeenha en laat pigmert f

eklorophyl n organic uolends ae shon to be
dese to embinaien. ocllorophyl asih prodetn inhe
lea
3. Jhe ebbeet ot Jomagenty on enraets ot he
inueskigated
eklorophl-n protein compcurd has ban
eo-lh stRong ocid ond olkali macirtep the.absenption
D
spectum aeld co'nweekina oempKund tothe
4 oluali spe saponidgino) tte_
phaeophugtn dastvatise
oi ehlonophill
estocddied grops
AX 9caklp aeid 3elutiens dermativw ho prolcin.
pH= 4-5) aunc maelid he alosenpien speelbum f
luoreseenee as eadie doseribed don Ho imbad Joah

h e protein is doralevead bydougina?.

os
Lo eome. ot aleohol en oeelone paaeipitate Ord
oderatuwee he proten; ehergingHe speebusm Ord oho
propesehes highes eoncentrediens caue disoeicdior
ESioikire? Jibereina) e pigment.
5 Deliagras such os, diatorin bila sall, ad
Sodium desezycholota elasibe) e leat eaacia
but doratoe tho protein ekanqing he
spackum
othas popetieg.

9nhilbtrro oqonts ob phoaBosumthesiy ataoitet ebteket

n the
absorphion speobum ob ehlenephll pretein
Compeumds.
She ed obsonption bend ot eklerophl poeses
he same eineticen salue n orgamie sevent j.e.
ethe on pesolewrm etha , and n
aguo leae
eaos elaitied b digitonin althouah Jhe bond
b
positiong o0 diuant
ho protein Comtent coas 46-57. yicldng9
an
aweaqo valea ob AG Pots pon doo dsot
D
proteinh.
Jhis emresponds a a ohle-ophulk eonmtond od boee
molaereles e elloropha 4 1 ol hloehlb
 he Svedberg unit ot 17,5O
9 t is sugcetad hat is nay 9epesent o
dodirite Combinine) Talio ot a 4b mtha protein
moleeulo.

AMPLES OF Hemeproteing
Here ase sOrne esarnples ard inborrolidn
okout he heme ptotein
. Hemoglobin t h e quankitatiely most importpm

hemoproten..
Casvien oi oxuggen in the blecd.
9t i
i it Jhe body conaing 60 qrems (t.6 peunds) ot
D
homoolobiri ; }t J3 eplaeecd aveu 40 da.
Nowmal hemsaleoin r a v e r 9agouire aythosi-

-zi'no 6-9 q ot new hornaglebir.daila a procesy

whieh wes albout 14. otha dielaouy amino acide

SOO mg ot heme ae rheized dail ben -

Coyugalion oth Ha neualp yrtheaizecl globín

D
otein.

|4 Cutocheame: Coehomes ate pesent inthe

miroohomdoüa 4 in Hhe endeplasmie selieulum(ee
in tHo imporat eloetreon
h a y poodieipaBe
remspomt hain(EIe) seaetions,

Jhoie hall lide is aboutta hor

8 rupBophar Cygenase uptophar
a
hemoproten ct mumadiasu) melabolism.
cata.ly zes tho eonsessicn ot 3uptophan
N-doorruykynwenine usin agno

he odant
about & hows.
A hak JHe i ony
h e ae Onhp a gew ob he many
- homoproteins.

4Catalas 4 eroridasehoy @aety coith

hydbeagern peoi de(H20).

ezemas
tha body oqainst
protect
hose
h e s e enymes
hycbtoqen pexoide.
uneonhroled
ovidation bp
D
s alaout hsus.
habe hall ide go

hose ao@ ony a dew ot ma

amp

ot theemoproteing