Buku Abstrak Isoc 2020 Fix

4th ISOC (International Seminar on Chemistry)
0
Welcome Speech from Chairperson of ISoC 2020
Assalamu’alaikumwarahmatullahiwabarokatuh,
Good Morning!
Honorable Rector of Institut Teknologi Sepuluh Nopember,
Distinguished Keynote and invited Speakers, participants, colleagues, ladies and gentlemen. It is
our great pleasure to warm welcome all of you to the 4th International Seminar on Chemistry
(ISOC) 2020, which theme “Hold The World with Chemistry”, organized by Department of
Chemistry, Faculty of Science and Data Analytics, Institut Teknologi Sepuluh Nopember (ITS). I am
very grateful to welcome to all keynote and invited speakers:
1. Prof. Geraldine Richmond, University of Oregon, USA.
2. Prof. M. Iqbal Choudhary, University of Karachi, Pakistan.
3. Prof. James Katudat Cairns, Suranaree University of Technology, Thailand.
4. Prof. Hamzah Fansuri, M.Si., Ph.D., ITS, Indonesia.
The 4th ISoC is a continuation of the 3nd ISoC, which has been held since 2018 with purpose to
promote and develop science and technology in chemistry. The ISoC itself is continuation of the
National Seminar on Chemistry (SENAKI), which has been held every year since 1999. ISoC is a
forum for academia, researchers, lecturers, educators, students (undergraduate and postgraduate), and
observers from universities, research institutions, industries and general public to exchange ideas and
latest information in the field of chemistry and its application. The conference is expected to foster
networking, collaboration and joint effort among the conference participants to advance the theory
and practice as well as to identify major trends in chemistry and its application.
We will spend about two days together for the conference. This conference is attended delegates
and contributors from Indonesia, Pakistan, USA, and Thailand. There are 146 papers presented in
this seminar, 142 to be presented orally and 4 papers by posters. Selected papers presented in the
conference will be published in the HAYATI Journal of Biosciences, International Journal of
Renewable Energy Development (IJRED) or AIP proceeding (both are indexed by Scopus).
The organizing committee would like to express our deepest appreciation to Rector ITS, Dean of
Faculty of Science and Data Analytics ITS, Head of department of Chemistry ITS, Keynote and
Invited speakers, and sponsors (Genecraft, Mitra gemilang, Raisha Collection, Science Hijab) for the
support, without all mentioned this conference may not be happened. Furthermore, my appreciation
goes to the members of the committee for their hard work and cooperative teamwork in the
preparation of the conference.
Finally, we wish all participants to enjoy a fruitful scientific discussions. Please feel free to ask
questions to committee members. We are here to serve you.
Thank you. Have a wonderful day.
Sri Fatmawati, M.Sc, Ph.D

Chairperson of the 4th ISOC 2020

1
Opening Speech from Rector of Institut Teknologi Sepuluh Nopember (ITS)
Assalamu’alaikumwarahmatullahiwabarokatuh,
Distinguished delegates! Our gratitude to Allah SWT, due to His blessing, we can continue
to perform activities and achieve a proud achievement, one of them by organizing International
Seminar on Chemistry (ISOC) 2020 with the theme of “Hold The World with Chemistry”. ISOC
is a continuation of the National Seminar on Chemistry (SENAKI) that has been held almost every
year since 1999 by the Department of Chemistry ITS. Implementation of the ISOC is now the fourth
time and will be held every two years. The first ISOC which was held in Bali in 2014.
On behalf of ITS, I congratulate the Department of Chemistry of ITS, as well as all the
participants in this seminar for their participation. I would like to express great appreciation to the
Keynote Speakers:
1. Prof. Geraldine Richmond, University of Oregon, USA.
2. Prof. M. Iqbal Choudhary, University of Karachi, Pakistan.
3. Prof. James Katudat Cairns, Suranaree University of Technology, Thailand.
4. Prof. Hamzah Fansuri, M.Si., Ph.D., ITS, Indonesia.
and also to invited speaker who had been taking the time to share their knowledges and experiences
to us. I also want to express my thanks and high appreciation to the entire committees and Department
of Chemistry of ITS for all their efforts so this international seminar event can be held.
As we know, more problems arise in the world where we live. Global warming due to
greenhouse gas emission, environmental problems triggered by population growth and increasing of
human life standards, increasing food demand and energy consumption, and also health problems,
were the major problems that closely related with the future and the survival of mankind. On the
other hand, the earth also has limitations for supporting the human life, especially in the term of
shortages of unrenewable resources and the decreasing of environment quality. Therefore, some
efforts should be done to solve the problems. Research and development should be improved to
optimize the natural resources exploration, also, exploration for obtaining alternative renewable
resources should be done to replace the unrenewable resources. Chemistry as one of the basic science
of course has an important role in these efforts. Discussion, exchange of ideas and collaboration
between researchers, educators, observer, and practitioners from all of Universities, research
institutions, industry and the public are such way to find the solutions, which in turn can contribute
to sustain our life in the future.
Finally, we really hope, may what we discussed in this seminar will contribute ideas to the
solutions of the complex problems that we are facing nowdays. By this event, we discuss together,
we work together in the same purpose, to get better life in the future.
Thank you for all of your attention. Enjoy the seminar!

Billahitaufik walhidayah, wassalaum’alaikum wa rahmatullahi wa barokatuh.
With best wishes

Surabaya, October 07, 2020

2
ISOC 2020 Agenda
ISOC 2020, Day 1 (07 October 2020) Link : http://bit.ly/isocday1 Password: F3QHYWNE
Time Agenda
07.30-08.30 Validation and zoom preparation
08.30-08.45 Schedule explication and national anthem "Indonesia Raya" and Hymne ITS
08.45-09.00 Welcoming speech from Chair of ISoC 2020
09.00-09.10 Welcoming remarks of Rector ITS and opening ceremony
09.10-09.15 Photo Session
09.15-09.55 Keynote 1 : Prof. Geraldine Richmond (University of Oregon, USA)
09.55-10.00 Token of Appreciation (virtual) by Vice Rector ITS
10.00-10.40 Keynote 2 : Prof. M. Iqbal Choudhary (University of Karachi, Pakistan)
10.40-10.45 Token of Appreciation (virtual) by Head of Department of Chemistry - ITS
10.45-11.00 Coffee Break (Ice breaker)
11.00-11.30 OWSD – Indonesia : Women in Chemistry
11.30-13.00 LUNCH BREAK
ORAL SESSION
Room 1 Room 2 Room 3 Room 4 Room 5 Room 6 Room 7
13.00-13.10 Invited 1 Invited 2 Invited 3 Invited 4 Invited 5 Invited 6 Invited 7
13.10-13.20 O20200017 O20200025 O20200027 O20200081 O20200001 O20200015 O20200105
13.20-13.30 O20200118 O20200031 O20200116 O20200095 O20200045 O20200103 O20200141
13.30-13.40 O20200004 O20200047 O20200177 O20200126 O20200164 O20200117 O20200149
13.40-13.50 O20200108 O20200065 O20200077 O20200135 O20200026 O20200145 O20200093
13.50-14.00 O20200063 O20200086 O20200053 O20200059 O20200170 O20200148 O20200138
14.00-14.10 COFFEE BREAK
14.10-14.20 O20200037 O20200143 O20200071 O20200021 O20200171 O20200056 O20200106
14.20-14.30 O20200085 O20200144 O20200062 O20200072 O20200151 O20200153 O20200110
14.30-14.40 O20200096 O20200161 O20200115 O20200082 O20200179 O20200061 O20200102
14.40-14.50 O20200057 O20200165 O20200146 O20200094 O20200130 O20200088 O20200109
14.50-15.00 O20200155 O20200052 O20200150 O20200114 O20200174 O20200147 O20200154
15.00-15.10 CLOSING DAY 1

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ISOC 2020, Day 2 (08 October 2020) Link : http://bit.ly/isocday2 Password: NTL2HO34
Time Agenda
08.00-09.00 Validation and zoom preparation
09.00-09.40 Keynote 3 : Prof. James Katudat Cairns (Suranaree Uniersity of Technology, Thailand)
09.40-09.45 Token of Appreciation (virtual) by Director of Research ITS
09.45-10.25 Keynote 4 : Prof. Hamzah Fansuri (Institut Teknologi Sepuluh Nopember, Indonesia)
10.25-10.30 Token of Appreciation by Chair of ISOC
10.30-11.00 Coffee Break (Ice breaker)
11.00-11.15 Indonesian Chemical Society (HKI): Chemistry in Indonesia
11.15-11.30 Indonesian Young Academy of Science (ALMI): Science in Indonesia
11.30-13.00 LUNCH BREAK
ORAL SESSION
Room 1 Room 2 Room 3 Room 4 Room 5 Room 6 Room 7
13.00-13.10 O20200034 O20200012 O20200180 O20200011 O20200162 O20200013 O20200119
13.10-13.20 O20200036 O20200038 O20200097 O20200067 O20200018 O20200078 O20200091
13.20-13.30 O20200040 O20200039 O20200158 O20200070 O20200167 O20200083 O20200055
13.30-13.40 O20200044 O20200008 O20200159 O20200076 O20200022 O20200090 O20200098
13.40-13.50 O20200113 O20200024 O20200160 O20200073 O20200163 O20200129 O20200112
13.50-14.00 O20200182 O20200099 P20200002 P20200003 P20200004 P20200005 O20200184
14.00-14.10 COFFEE BREAK
14.10-14.20 O20200087 O20200042 O20200181 O20200054 O20200041 O20200127 O20200069
14.20-14.30 O20200033 O20200046 O20200029 O20200084 O20200066 O20200137 O20200064
14.30-14.40 O20200104 O20200173 O20200176 O20200183 O20200075 O20200142 O20200166
14.40-14.50 O20200125 O20200019 O20200178 O20200185 O20200035 O20200156 O20200023
14.50-15.00 O20200172 O20200043 O20200175 O20200186 O20200030 O20200133
15.00-15.10 CLOSING DAY 2

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CONTENTS
Welcome Speech from Chairperson of ISoC 2020 ................................................. 1

Opening Speech from Rector of Institut Teknologi Sepuluh Nopember (ITS) .... 2
ISOC 2020 Agenda................................................................................................... 3
CONTENTS ............................................................................................................. 5
KEYNOTE SPEAKER .......................................................................................... 15
“Understanding Environmentally Important Processes at Water Surfaces” .............. 16
Biocatalytic, Phytochemical, and Synthetic Chemistry Approaches in Hitto- Lead in
Drug Discovery- Highlights of our Recent Work ..................................................... 17
Chemical Applications of Beta-Glucosidases and Related Enzymes ........................ 18
Development of Perovskite-based Membrane Catalyts for Methane Partial Oxidation
in ITS ....................................................................................................................... 19
INVITED SPEAKER............................................................................................. 20
Methyl Orange Dye Transformation by Brown-Rot Fungi ....................................... 21
Paper-based Analytical Devices (PADs) and Their Analytical Applications ............ 22
Amine Functionalized Mesoporous Carbon as CO2 Storage Materials ..................... 23
Unfolding Diversity of Carotenoid Pigments in Indonesian Unique Species ............ 24
Advanced Design Photocatalyst for Waste Water Remediation by Aerosol Process 25
Phytochemical Characterization and in vitro Biological evaluation of phytochemical
from Diospyros lotus ............................................................................................... 26
ORAL PRESENTER ............................................................................................. 27
Biodegradation of DDT by Co-cultured of Pleurotus eryngii and Pseudomonas
aeruginosa ............................................................................................................... 28
Antibacterial and Toxicity Activities of Indonesian herbal medicine extracts used for
postpartum treatment ............................................................................................... 29
Polyol from Epoxidized Candlenut Oil (Aleurites Moluccanus) and Poly Ethylene
Glycol (PEG) 200 : Kinetic Study ............................................................................ 30
The Beneficiacion of Sumbawa Manganese Ore using Response Surface Method
(RSM) ...................................................................................................................... 31

5
Development of PVDF/LiCl Polymeric Membrane for Harvesting of
Nannochloropsis sp. ................................................................................................. 32
Application of Ionic Rectification for Water Purification: Desalination with a Nafion
Cationic Diode ......................................................................................................... 33
Analysis of Heavy Metals (Pb, Cd, Cu and Cr) with Inductively Coupled Plasma
Spectrometer (ICPS) on Cracker of Sea Cucumber (Phylloporus spiculatus) at
Kenjeran, Surabaya, Indonesia ................................................................................. 34
Anti-cancer Activity of Okra Polysaccharides on Human Liver Cancer Huh7it Cells
Growth and Natural Killer Cell ................................................................................ 35
Characteristics of Edible Film Derived from Amylose Fraction of Sago Starch ....... 36
Cracking of Jatropha Oil into Biofuel over Hierarchical Zeolite Supported NiMo
Catalyst Catalytic ..................................................................................................... 37
Synthesis and Characterisation of Magnetic Nanoparticles Fe/Fe Oxide of Sodium
Borohydride Reduction Results within Chitosan Hydrogel ...................................... 38
Effect of Catalys and Cross Linker Concentrations on The Chemical Properties of
Sago Starch .............................................................................................................. 39
Green Synthesis of Au-Doped ZnO NANOPARTICLES (ZnO:Au NPs) by In-situ
Process using Imperatacylindrica L Leaf Extract ..................................................... 40
Surface Modification of Nanoporous Carbon Using Gamma Irradiation Treatment as
Supercapacitor Material ........................................................................................... 41
Improvement N2/SF6 Separation Performance on P84 Derived Carbon Membrane by
Incorporating of Zeolite-Carbon Composite ............................................................. 42
Effects of Temperature and Neutralizing Agent on Lactic Acid Production by
Rhizopus sp. Fermentation ....................................................................................... 43
Synthesis and Characterization of ZnO Nanoparticles Using Pumpkin Seed Extract
(Cucurbita moschata) by the Sol-Gel Method .......................................................... 44
Chemical Characteristic of Fly Ash from 3 Kalimantan’s Power Plants as Potential
Source for Synthesis of Aluminosilicate-Based Material ......................................... 45
Optimization Topical Cream of Nanoemulsion Bromelan from Pineapple Core as
Antiinflamation and Antibacterial of Propionbacterium Acnes with In Vitro Testing
in Animals Test ........................................................................................................ 46
Irradiation Grafting of Hydrophilic Monomer onto Chitin ....................................... 47

6
Mineral, nutrition and phytochemical of ethnomedicine of Parkia Roxburghii G.Don
................................................................................................................................. 48
Active Methylene Variations on 3-Substituted Coumarin Synthesis ........................ 49
Simulation of Modified Sorghum Flour using Lactobacillus Plantarum Bacteria .... 50
Synthesis of Vanillyl Alcohol Derivatives ............................................................... 51
Phenolic Compounds from The Rhizomes of Indonesian Mango Ginger (Curcuma
amada)..................................................................................................................... 52
Adsorption of Carbon Dioxide in Porous Carbon Containing Monoehtanolamine
(MEA) : The Effect of Carbon Surface Pretreatment ............................................... 53
Adsorption of Lithium and Calcium-Containing Solution for Seperation Application
................................................................................................................................. 54
Optimization Study of Alkyl Polyglycoside C12 Synthesis using Indirect Method .. 55
Physiogenetik And Root Architecture of Tobacco Plants (Nicotiana tabacum) Under
Periodic Waterlogging Stress ................................................................................... 56
Influence of Binders on Properties of Biomomass Briquettes from Durian peel (Durio
kutejensis Becc) ....................................................................................................... 57
Influence of Particle size of Lai peel (Durio kutejensis Becc) charcoal on Properties
of Biomomass Briquettes ......................................................................................... 58
Synthesis of Hemiterpene 1-Bromo-3-Methyl-2-Butene Derivatives ....................... 59
Interpenetrated Polymer Network of Bacterial Cellulose ......................................... 60
Biodiesel Synthesis from Waste Cooking Oil and using CaO derived from green
conch shell waste and limestone as Catalyst by Microwave ..................................... 61
Modification of Rubber Seeds (Hevea brasiliensis) as Adsorbent to Improve The
Quality of Used Fried Cooking Oil .......................................................................... 62
Effect of pH, Temperature, and Electrolytes on Swelling and Release Behaviors of
PVA/AAM/GO Based Hydrogel Composities ......................................................... 63
Characterization of Mineral Content from Puntaru Beach Iron Sand........................ 64
New QSPR Study for Predicting Critical Micelle Concentration of Gemini Cationic
Surfactants Using the Online Chemical Modeling Environment (OCHEM) ............. 65
The Effect of Amine Types on Breakthrough Time for CO2 Gas Seperation
Performance ............................................................................................................. 66

7
Differential Pulse Voltammetry Study for Quantitative Determination of Disporsium
(III) in Acetonitril Solution ...................................................................................... 67
Phytochemical and Antioxidant Activities of Robusta Coffee Leaves Extracts from
Alor Island, East Nusa Tenggara .............................................................................. 68
Bioluminescence Study of Red Dragon Fruit (Hylocereus polyrhizus) Extract in
Various Solvents ...................................................................................................... 69
Detection of Triethylamine on Supramolecular 3-[(E)-(4-Acetylphenyl)Diazenyl]-4-
Hydroxybenzaldehyde Compound ........................................................................... 70
Synthesis of Solid and Hollow TiO2 Nanofibers with Electrospinning Method ........ 71
Hepatoprotective Effect of Crude Extract Polysaccharide Ganoderma applanatum on
Carbon Tetrachloride-induced Liver Fibrosis in Mice .............................................. 72
Microencapsulation of Gadolinium Citrate Using Silica for Contrast Agent
Development and Its Encapsulation Efficiency Determination................................. 73
Synthesis and Characterization of Ion Imprinted Polymer for Selective Separation of
Cd(II) ....................................................................................................................... 74
Zeta Potential Examination of β-Carotene Encapsulated In Starch-Chitosan/Tripoly-
phosphate Microparticles ......................................................................................... 75
Response Surface Methodology of Calcite Precipitation using Sodium
Lignosulfonate and Polyaluminium Chloride ........................................................... 76
The Effect of Stannous Chloride Catalyst in the Formation of Pla from DL-Lactic
Acid as an Eco-Friendly Plastic Raw Material ......................................................... 77
Methylene Blue Removal from Waste Water Using Sodium Lignosulfonate and
Polyalumunium Chloride: Optimization With RSM ................................................. 78
The Effect of Precursors on Synthesis of TiO2 and Their Photocatalytic Activity on
Degradation of Phenol ............................................................................................. 79
Molten Salt Synthesis of Photocatalyst Material SrTix-1FexO3 (x= 0, 0.05, 0.1, 0.15,
and 0.2) .................................................................................................................... 80
Multianalytical characterizations for investigating the origin of prehistoric rock art
pigments of Karim Cave, Sangkulirang, East Kalimantan, Indonesia ....................... 81
Characteristics of Fish Protein Concentrate Extracted from Snakehead fish (Channa
striata) using Two Different Extraction Methods ..................................................... 82

8
Introducing Closed Form Solution of Riccati Differential Equation of Constant
Coefficient for Solving Some Model of Chemical Processes ................................... 83
Optimization of Zinc Oxide Nanoparicles Biosynthesis Using Saccharomyces
Cerevisiae with Box-Behnken Design ..................................................................... 84
Compisition and Density of Marine Litter in Alor Island of Nusa Tenggara Timur.. 85
Characteristics of Cepu Block Oil, Wonocolo Formation, East Java Indonesia : Study
of aliphatic biomarkers ............................................................................................ 86
Synthesis and Characterization of Cr-Co/γ-Alumina Catalyst for Ethanol Dehydration
................................................................................................................................. 87
Synthesis, Characterization, and Identification of Metallic Soap (Zn-, Al-, and Mg-
Soap) and Its Potential as a Sunscreen ..................................................................... 88
“SIAB” Smart E-Stoichiometry As A Chemistry Learning Acid Base On Android
Mobile ..................................................................................................................... 89
Terpenoid from Indonesian Temu Mangga (Curcuma mangga, Val) Rhizomes and
Review of Its Anticancer Towards MCF-7 Breast Cells ........................................... 90
Physicochemical Properties of Microcrystalline Cellulose Doped Imidazole
Membrane for Direct Methanol Fuel Cell ................................................................ 91
Alkaloid and Terpenoids From Indonesian White Turmeric (Curcuma zedoaria)
Rhizomes and Its Potency as Antibacterial Agent .................................................... 92
Physicochemical Properties of Prehistoric Rock Art Pigments in Jupri Cave,
Sangkulirang, Indonesia ........................................................................................... 93
Detailed studies of prehistoric rock art pigments in Tewet Cave, Sangkulirang site,
East Kalimantan, Indonesia ...................................................................................... 94
Green Coke Graphite as Anode in Lithium Ion Battery ............................................ 95
Intramolecular Hydrogen bond and Vibrational Spectroscopic Study of Cellulose
Oligosaccharide Using Density Functional Theory .................................................. 96
Reactive Oxygen Species (ROS) Hydrogen Peroxide Scavenger Activities of Dimeric
Complex (µ-4,4'-Bipyridine)-Bis{Perchlorate-Salen-Manganese(III)} Through
Vitamin C Inhibition ................................................................................................ 97
Kinetics of Citronella Oil Extraction from Cymbopogon nardus Leaves using
Microwave-Assisted Green Technique .................................................................... 98
Isolation of Cellulose from Teak Wood Using Hydrothermal Method ..................... 99
9
Synthesis of NiCl2 Impregnated Mesoporous Carbon and Its Adsorption Activity on
CO2 ........................................................................................................................ 100
The Effect of Alkaline Concentration in the Alkaline Delignification-Assisted
Sonication on Sengon Wood .................................................................................. 101
Synthesis of Bimetallic Ni-Co Modified Mesoporous Carbon and Its Catalytic
Activity for Selective Hydrogenation of Phenylacetylene to Styrene ..................... 102
Optimization of Non-Catalytic In-Situ Biodiesel Production from Rice Bran Using
Historical Data Design (HDD) ............................................................................... 103
Analysis Of Cd Metal Ions Using Screen Printed Electrode (SPE) Modified With 4-
Carboxyphenyl Diazonium Salt ............................................................................. 104
IIn Vitro Free Radical Scavenging Activity of Flavonoid Glycoside Compounds from
The Leaf Extract of Protium Javanicum Burm. F .................................................. 105
Computational Investigation of Interaction between β-Cyclodextrin and
Hydroxychloroquine .............................................................................................. 106
Theoretical Investigation of Interaction between Hydroxypropyl-β-Cyclodextrin and
Hydroxychloroquine .............................................................................................. 107
Antibacterial Activity of Flavonoids from Ethyl Acetate Extract of Milk Banana
Peel(Musa x paradisiaca L.) .................................................................................. 108
Investigation of Envelope Protein Gene in Indonesian Severe Acute Respiratory
Syndrome Coronavirus 2 Isolates: A Bioinformatics Study ................................... 109
A comparison of nine different models to correlate the solubility of azobenzene
compounds in supercritical carbon dioxide ............................................................ 110
Oleic-Imidazoline Synthesis and Its Performance as Corrosion Inhibitor towards
Carbon Steel .......................................................................................................... 111
Microwave Assisted Synthesis of 4-Methyl Coumarin Derivatives from Various
Dihydroxybenzene ................................................................................................. 112
Zinc Ferrite Nanoparticles Synthesized Via Hydrothermal Method Using Simbang
Darah Extract as Capping Agent and Their Activities ............................................ 113
Cascade Charge Transfer on Nanostructured BiVO4/TiO2/Dye/Co-Catalyst for Water
Oxidation ............................................................................................................... 114
Synthesis Of Zinc Oxide (ZnO) Nanoparticles By Polyol Method And It’s
Application On Photocatalytic Reduction Of Paracetamol Concentration .............. 115

10
Determination of Several Heavy Metals in Staple Foods from Traditional Markets in
Capital City of Indonesia using Neutron Activation Analysis ................................ 116
Antioxidant of Beta-Carotene Emulsion from Red Virgin Palm Oil (RVPO) ......... 117
Effect of Operating Conditions on The Flux Recovery of Lithium From Geothermal
Brine Using Forward Osmosis ............................................................................... 118
Microwaved-assisted Extraction from Microalgae Chlorella Sp. for Biodiesel Raw
Materials ................................................................................................................ 119
Kinetics Study of Microwave Hydrodestillation (MHD) Fresh and Dried
Cymbopogon Nardus Leaves ................................................................................. 120
Chemical Pre-treatments Effect for Reducing Lignin on Cocoa Pulp Waste for
Biogas Production .................................................................................................. 121
Application of The Multi Soil Layering (MSL) System to Treat Laboratory
Wastewater ............................................................................................................ 122
Immobilization of Trypsin onto Porous Monolith Methacrylate on High-Performance
Liquid Chromatography Applications .................................................................... 123
The Combination of Natural Dyes from Jengkol Pod Waste (Archidendron jiringa)
and Liquid Waste of Gambier (Uncaria gambir Roxb) on Coloring of Cotton Fabrics
............................................................................................................................... 124
Isolation and Molecular Docking Study of A Novel Ergone Steroid from Endophytic
Fungus Aspergillus Carbonarius of Sponge Aaptos Suberitoides........................... 125
Optimization of Lignin Removal Pre-Treatment Process in Acetone-Butanol-Ethanol
(ABE) Production from Empty Fruit Bunches of Oil Palm .................................... 126
Computational Study of Nickel Doped ZnO Cluster for Cationic and Anionic Dye127
Calculations of Linoleic and Linolenic Acid Esterification Using ZnO Catalyst DFT
............................................................................................................................... 128
The Effect of Stirring and Fermentation Time of Coconut Cream on The Yield and
Quality of Virgin Coconut Oil (VCO) .................................................................... 129
Annealing Treatment for Enhancing of H2/C3H8 Separation Performance on
Polysulfone Membrane .......................................................................................... 130
Synthesis and Characterization of Thermosensitive-Ionic Based Gel in Various
Temperatures ......................................................................................................... 131

11
Study on Electrochemical Analysis of Uric Acid using Screen Printed Electrode
(SPE) Modified Co, Ni, and Co/Ni ........................................................................ 132
Synthesis And Phase Transformation Of Hydroxyapatite From Indonesian Natural
Sources .................................................................................................................. 133
Adsorbent From Bagasse To Trap The Red Procion Substance In Batik Waste ..... 134
Modification of Gold Electrodes Using 3-Mercaptopropionic Acid and EDTA as a
Copper Sensor Application. ................................................................................... 135
Functionalization Single-Walled Carbon Nanotubes with a HNO3/H2SO4 Mixtures
Through Different Treatments: A DFT Supported Study ....................................... 136
Dry Mechanosynthesis and Characterization of Carbonate Apatite Based on
Indonesian Natural Sources.................................................................................... 137
Isolation, Selection and Identification of Sulphur Oxidizing Bacteria (SOB) from
Sludge of Tofu Industry Wastewater ...................................................................... 138
Adsorption of Mercury (II) From Aqueous Solution By Crumb Rubber Sludge:
Isotherm and Kinetic Studies. ................................................................................ 139
Alkali Metal Doped ZnO Nanoclesters for Dye Photodegradation: Computational
Studies ................................................................................................................... 140
Non Polar Extracts from Stem Bark of Garcinia lasoar against Staphylococcus aureus
............................................................................................................................... 141
Effect of Drying Temperature on Characteristics of Gelatin from Nila Fish
(Oreochromis niloticus) Skin ................................................................................. 142
Synthesis and Characterization of NaY Zeolite From Capkala Kaolin with Variation
Crystallization Time .............................................................................................. 143
Synthesis of Zeolite A Supported Fiber and Its Aplication for CH 4 Adsorption ..... 144
The Effect of Different Type of Additional Silica Source on The NaY Zeolite
Synthesis from Bangka Belitung ............................................................................ 145
Preparation of Molecular Imprinted Polymer (MIP) for Selective Extraction of Gallic
Acid ....................................................................................................................... 146
Analysis of Antioxidant Activity of Black Tea Extract (Camellia sinensis L.) and the
Effect on Organoleptic Properties of Cassava Getuk .............................................. 147
Effect of Addition Black Tea Extract (Camellia Sinensis) on Antioxidant Activity of
Pukis Cake ............................................................................................................. 148
12
Biodecolorization and Biotransformation of Methylene Blue by Mixed Cultures of
Brown-rot Fungus Daedalea dickinsii and Filamentous Fungus Aspergillus oryzae
............................................................................................................................... 149
PVDF/LiCl Membrane For Up-Concentration Of Nannochloropsis sp. Microalgae
Harvesting And Its Cleaning .................................................................................. 150
Content and Morphology of Skeletonema Costatum on Nutrient N and Si Stress Lipid
............................................................................................................................... 151
WonoYo: Improving Local Dairy Product Potential by Combining Fortification of
Yogurt Fermentation and Online Branding Marketing ........................................... 152
Biotransformation of Methylene Blue by Mixed Fungal Cultures of Gloeophyllum
trabeum and Aspergillus oryzae ............................................................................. 153
Biodecolorization and Biotransformation of Methylene Blue by Mixed Cultures of
Brown-rot Fungus Gloeophyllum trabeum and Filamentous Fungus Trichoderma
viride ..................................................................................................................... 154
The Antibacterial activity of Vanillin Derivative Compounds ............................... 155
Catalytic Cracking Polystyrene Waste by Co-Reactant Biodiesel Waste Coconut Oil
and Its Performance at Gasoline Engine ................................................................. 156
The Ability of Mixed Cultures Daedalea dickinsii and Trichoderma viride on
Biodecolorization of Methylene Blue Dye ............................................................. 157
A New Route Synthesis of Al-MCM-41 From Red Mud Waste for Reutealis
Trisperma Oil Deoxygenation ................................................................................ 158
The Effect of Various Templates for Synthesis Aluminosilicate as Catalysts on Oleic
Acid Deeoxygenation Reaction .............................................................................. 159
The Effect of Variation Linker’s Amount on Synthesis of Mesoporus ZnO for
Photodegradation of Methylene Blue ..................................................................... 160
The Adsorption and Photocatalytic Activity Test of ZSM-5 for Methylene Blue
Decolourization ..................................................................................................... 161
Bio-degradation of Methylene Blue (MB) by Ralstonia pickettii ........................... 162
Synthesis of ZIF-67 in The Presence of Acetic Acid as Methyl Orange Adsorbent 163
Synthesis of Al-HKUST-1 and Its Application as A Congo Red Adsorbent........... 164
Synthesis of Indolin-2-One Derivatives and Their In Vitro Anticancer Activity
Against Widr Cell Line .......................................................................................... 165
13
Isolated Compound from Stachytarpheta jamaicensis Leaves................................ 166
Antioxidant Activity of Rhodomyrtus tomentosa (W. Ait) Hassk and Melastoma
malabatchricum L. Leaf Extract ............................................................................ 167
Optimization of Bioactive Compounds of Capsicum annuum ................................ 168
Terpenoid from Chromolaena odorata Leaves ...................................................... 169
POSTER PRESENTER ....................................................................................... 170
Copper (Cu) Content in Sea Water and Sediment and Its Bioavailability in Balangan
Beach Bali ............................................................................................................. 171
Novel Fast Firing Method to Synthesiza Tetracalcium Phosphate .......................... 172
4-Carboxy Phenyl Diazonium Modified Carbon Paste Electrode (CPE) for Cu(II)
Analysis ................................................................................................................. 173
Sonication-Assisted Green Synthesis of Silver Nanoparticles Using Aqueous Extract
of Piper retrofractum Fruits and Their Antimicrobial Activity .............................. 174

14
KEYNOTE SPEAKER

15
“Understanding Environmentally Important Processes at Water Surfaces”
Geraldine Richmond
Presidential Chair and Science and Professor of Chemistry
University of Oregon
Keynote Speaker
ABSTRACT
Although the special properties of water have been valued and appreciated for centuries, as scientists
we continue to be perplexed by the molecular make-up of water in all its forms. Equally perplexing
is the surface of water, the entry gate for anything going into our water. This presentation will
highlight what we have learned in our laser and theoretical studies about the intriguing molecular
characteristics of a water surface and how its behavior plays a role in environmentally important
processes.

16
Biocatalytic, Phytochemical, and Synthetic Chemistry Approaches in Hitto-
Lead in Drug Discovery- Highlights of our Recent Work
M. Iqbal Choudhary and Atta-ur-Rahman FRS
Ministerial Standing Committee for Science and Technology Cooperation in OIC Countries,
33 Constitution Ave, Islamabad, Pakistan
International Center for Chemical and Biological Sciences, University of Karachi, Karachi-75270, Pakistan
Email: Iqbal.choudhary@iccs.edu
Keynote Speaker
ABSTRACT
As developing world face food security issues, it also confronts with the drug insecurity challenges. The
current drug development paradigm is cost, time and labor intensive, which developing countries cannot
afford to invest. Therefore, there is a need of appropriate, efficient, and cost-effective strategies for drug
development, which must be based on indigenous knowledge resource base, S&T capacity, and people-
friendly approval process. In the last two decades, we identified a large number of new- and novel
chemical substances, and their synthetic analogues with potent activities against various disease- related
targets.
During last four decades, our research has been focused on the discovery of chemical constituents from
medicinal plants used in traditional medicines as well as identifying new biotransformed products with
therapeutic potential. This has resulted in the identification of several novel and new lead molecules
against various therapeutic targets. During this presentation, some recent examples of our studies on
bioactive natural products and biotransformation will be presented.
Breast cancer is the most common cancer in women worldwide, with nearly 1.7 million new cases
diagnosed in 2014 (second most common cancer overall). This represents about 12% of all new cancer
cases, and 25% of all cancers in women. Incidence rates vary from 19.3 per 100,000 women in Eastern
Africa to 89.7 per 100,000 women in Western Europe. In most of the developing regions the incidence
rates are below 40 per 100,000. It has been reported in 2015 as the most prevalent cancer in women.
Approximately one-third of all breast cancer patients, and two-thirds of postmenopausal breast cancer
patients have hormone-dependent (estrogen-dependent) breast cancers, which express estrogen receptors,
and require estrogen for tumor growth. Aromatase inhibitors are currently being tested as primary
prevention therapy in large randomized trials. While only a few chemotherapies such as exemestane and
tamoxifen are in clinical practices for the inhibition of aromatase functions. Therefore, there is a need to
identify new structural analogues of available drugs, and evaluate their anticancer potential. Recently, we
have synthesized the new derivatives of existing aromatase inhibiting drugs through biotransformation,
and via conventional synthesis to evaluate their potential against aromatase enzyme. Besides, we have
also synthesized a number of new structural analogues of aromatase inhibitors, and other steroidal drugs,
including exemestane, formestane, testolactone, mibolerone, boldenone, indomethacin, levonorgestrel
through biotransformation. This has provided an efficient method for the synthesis of new analogues of
existing aromatase inhibitors. The new analogues of the mentioned drugs were found to be moderate to
potent inhibitors of aromatase enzyme, as compared to reference drugs letrazole, and exemestane.
Glycation is a nucleophilic reaction which leads to post-translational modifications of biomolecules (e.g.,

proteins, lipids, nucleic acids, etc.), and results in the long-term complications in diabetes. Several
synthetic and natural compounds were evaluated for their antiglycating ability such as indolelinked
triazoles, carbazole-linked triazoles, polyphenols, cyclopeptide alkaloids, steroids, coumarins, flavonoids,
etc.
During this presentation, underlying philosophy and approach of our research on cost-effective discovery
of lead molecules will be discussed.
17
Chemical Applications of Beta-Glucosidases and Related Enzymes
James R. Ketudat Cairns
Keynote Speaker
ABSTRACT
School of Chemistry, Institute of Science & Center for Biomolecular Structure, Function and
Application, Suranaree University of Technology, Nakhon Ratchasima 30000, THAILAND
Laboratory of Biochemistry, Chulabhorn Research Institute, Bangkok 10210, THAILAND
Beta-glucosidases (E.C. 3.2.1.21) catalyze hydrolysis of the glycosidic bond connecting a
nonreducing terminal glucosyl residue to a carbohydrate or aglycone and play a myriad of roles in
living organisms. We have investigated beta-glucosidases from various glycoside hydrolase families
via functional and structural studies, with the most emphasis on enzymes from families GH1 and
GH116. While studying GH1 enzymes, we found that rice Os9BGlu31 acts as a transglycosidase
more than as a hydrolase and identified several mutants that have higher activity and broader
substrate specificity than the parent enzyme. These allowed us to synthesize various
glucosylconjugates for use in our research, including phytohormone glucoconjugates that we could
use to study the enzymes responsible for metabolizing them. While investigating the catalytic
residues of Thermoanaerobacterium xylanolyticum TxGH116 beta-glucosidase, we found that its
catalytic acid/base and nucleophile mutants could efficiently produce glucosylazides that could be
used to produce glucosyltriazoles. Some of these compounds were effective inhibitors of human
alpha-glucosidase or bacterial beta-glucosidases, suggesting this could be a promising method to
develop new drugs. These cases provide good examples of how basic research can lead to production
of novel compounds for further application.

18
Development of Perovskite-based Membrane Catalyts for Methane Partial
Oxidation in ITS
Hamzah Fansuri
Department of Chemistry, Institut Teknologi Sepuluh Nopember, Faculty of Science and Data
Analytics,Sukolilo 60111, Surabaya, Indonesia
h.fansuri@chem.its.ac.id
Keynote Speaker
ABSTRACT
Methane is an important component in natural gas and biogas. Methane is a flammable organic substance.
The abundance of methane in nature is quite high and can be made chemically from other materials such
as coal or biomaterials as biogas. The result of burning methane as an energy source is much cleaner than
coal, so that methane hass very potential as a future energy source. Unfortunately methane is very difficult
to capture and store. Converting methane into other fuels with a higher boiling point such as methanol,
either directly or indirectly, is an effort that has been developed to address the drawbacks of methane
utilization. One method of conversion is by partial oxidation using metal oxide based catalysts. However,
besides being able to catalyze the oxidation reaction of menthane to methanol, the catalyst can also
completely oxidize methane and methanol into water and carbon dioxide. The main cause of complete
oxidation is the presence of gaseous oxygen in the reaction system. The complete oxidation of methane
and methanol by oxygen is thermodynamically more spontaneous. The enthalpies of the complete
oxidation of methane and methanol are −882.0 and −715.0 kJ/mol, respectively, compared to the partial
oxidation of methane to methanol or syn gas (a mixture of hydrogen and carbon monoxide gas, which is
the raw material for the synthesis of various hydrocarbons) are -126.4 and -36.0 kJ/mol, respectively.
Therefore, control of partial oxidation reactions cannot be carried out thermodynamically, but have to be
done kinetically by limiting the oxygen involved in the reaction.
Perovskite oxide-based catalyst membranes can be used to control the oxygen supply in the methane
partial oxidation reaction because the reaction use lattice oxygens of the perovskite as the sole source of
oxygen for the partial oxidation reaction. Oxygen is only available on the perovskite oxide surface.
Methane in direct contact with the membrane surface are the only molecules that will be oxidized so that
the partial oxidation reaction can be controlled through: i) frequency of contact between methane
molecules and the perovskite oxide surface and ii) the rate of reduction-reoxidation processes in the
perovskite oxide structure. However, there are problems and obstacles in the development and application
of perovskite oxide-based catalyst membranes. These problems include: i) the difficulty of sealing the
membrane in the reactor so that it does not leak; ii) the thickness of the membrane of choice is very limited
and iii) the reactivity of perovskite oxides at reaction temperatures ranging between 650 and 950 oC. This
discussion will reveal the research journey of perovxite oxide-based membrane catalysts at ITS as a
membrane catalyst for methane oxidation reactions. Topics to be pesented include the synthesis of
perovskite oxides, the manufacture of membranes, both in flat and hollow fibers configuration; and iii)
the characterization and performance testing of the membranes.

19
INVITED SPEAKER

20
Methyl Orange Dye Transformation by Brown-Rot Fungi
Adi Setyo Purnomo, Refdinal Nawfa, Surya Rosa Putra
Department of Chemistry, Faculty of Science and Data Analytics, Institut Teknologi Sepuluh Nopember
(ITS), Kampus ITS Sukolilo, Surabaya 60111, Indonesia
adi_setyo@chem.its.ac.id
Invited Speaker
ABSTRACT
An investigation was conducted to assess the ability of three species of brown-rot fungi to decolorize
and transform methyl orange dye. Methyl orange (MO) was decolorized in a potato dextrose agar
(PDA) medium by Daedalea dickinsii, Fomitopsis pinicola, and Gloeophyllum trabeum at different
concentrations of 50, 75, and 100 mg L−1. Based on the values of the decolorization index (DI), the
highest MO decolorization was found approximately 91% by F. pinicola, followed by D. dickinsii
and G. trabeum of 82% and 76%, respectively, at a concentration of 50 mg L −1. F. pinicola had the
highest MO transformation with percent decolorization values of approximately 97%, followed by
D. dickinsii and G. trabeum of 93% and 67%, respectively, after a 14-day incubation period in potato
dextrose broth (PDB). G. trabeum transformed MO into five metabolic products: compounds 1, 2, 3,
4, and 5, while F. pinicola transformed MO into six metabolic products: compounds 3, 6, 7, 8, 9, and
10. Among brown-rot fungi, D. dickinsii had more metabolic products, with compounds 3, 4, 6, 11,
12, 13, 14, 15, 16, 17, and 18. Based on the identification of metabolic products, novel bio-
transformation was proposed that brown-rot fungi initially transformed MO via three pathways: (1)
demethylation, (2) desulfonylation, and (3) hydroxylation. This study indicated that brown-rot fungi
can be used to decolorize and transform MO dye as well as proposed novel transformation of MO by
brown-rot fungi.
Keywords: Methyl Orange; Dye; Fomitopsis pinicola; Gloeophyllum trabeum; Daedalea dickinsii.

21
Paper-based Analytical Devices (PADs) and Their Analytical Applications
Akhmad Sabarudin
Department of Chemistry, Faculty of Science, Brawijaya University, Indonesia
Research Center for Advanced System and Material Technology, Brawijaya University, Indonesia
sabarjpn@ub.ac.id
Invited Speaker
ABSTRACT
Paper-based Analytical Devices (PADs) are a simple analytical platform that satisfies a combination
of low cost, portability, and easy-to-use. The PADs consist of two components, such as a paper and
a liquid barrier. The hydrophilic area in the PADs is used to load reagents and samples. The
hydrophobic barrier is created by constructing a pattern using water-insoluble material on a paper
substrate to provide a leak-proof barrier of the liquid path in the hydrophilic area of the PADs. In
these works, we will discuss the preparation of PADs and their applications to analytical and
bioanalytical chemistry, i.e., analysis of metallic elements, cystatin C, serum creatinine, hepatitis B
virus (HPV), and other applications discussed later.
Keywords: PADs, microfluidic, paper, wax printing, quantitative analysis.

22
Amine Functionalized Mesoporous Carbon as CO2 Storage Materials
M Faisal, A Z Pamungkas, Y K Krisnandi*
Solid Inorganic Framework Laboratory, Department of Chemistry, Faculty of Mathematics and Natural
Science, Universitas Indonesia, Depok 16424
yuni.krisnandi@sci.ui.ac.id
Invited Speaker
ABSTRACT
Mesoporous carbon has been successfully synthesized through soft template method with Pluronic
F-127 as pore directing agent; phloroglucinol and formaldehyde as carbon sources. Furthermore, the
surface of mesoporous carbon was modified with ethylenediamine (EDA) and triethylenetetraamine
(TETA) to enhance its capability as CO2 storage material. As comparison, microporous activated
carbon was also modified with TETA and tested its capability as CO2 storage material. The effect of
certain percentages of additional EDA (50 and 100 wt.%) or TETA (10, 30 and 50 wt.%) immobilized
into the surfaces of mesoporous carbon and activated carbon and its CO2 adsorption capability were
investigated. The characterization shows that EDA or TETA has been successfully incorporated
inside the pore. The CO2 adsorption test shows that for MC-EDA50, the CO2 adsorption capability
are significantly increased along CO2 flow time, whereas the CO2 adsorption capability of MC-
EDA100 is not as good as MC. Higher amount of EDA may provide more amine functional groups
to react with CO2, but it may also block CO2 diffusion into the pore of MC-EDA100. The CO2
adsorption test also shows higher percentage of TETA incorporated induced higher CO2 adsorption
capability, until it saturated in MC-TETA50. The highest CO2 adsorption capability (69%) was given
by MC-TETA30 within 30 minutes adsorption. The improvement of CO2 adsorption capability at
low TETA loadings (10 wt.% and 30 wt%) proved that its four amine functional groups gave
advantages for CO2 adsorption. On the other hand, incorporation of TETA into activated carbon is
not beneficial at all and reached a CO2 adsorption capability about 13.2% of the parent activated
carbon.
Keywords: Mesoporous Carbon, Activated Carbon, Ethylenediamine, Triethylenetetramine, CO 2,

23
Unfolding Diversity of Carotenoid Pigments in Indonesian Unique Species
Tatas Hardo Panintingjati Brotosudarmo
a
Ma Chung Research Center for Photosynthetic Pigments (MRCPP) and Department of Chemistry,
Universitas Ma Chung, Jl. Villa Puncak Tidar N-01, Malang 65151, East Java, Indonesia
b
Laboratory for Soft Materials Spectroscopy, University of Bayreuth, 95440 Bayreuth, Germany
c
Akademi Ilmuwan Muda Indonesia (ALMI), Jalan Medan Merdeka Selatan No. 11, Senen, Gambir,
Jakarta Pusat 10110, Indonesia
tatas.brotosudarmo@machung.ac.id
Invited Speaker
ABSTRACT
Natural pigments are the most obvious and eye-catching substances that can be found in vegetable
leaves, fruits, flower, seaweeds, microalgae, and many more. Carotenoids are a family of yellow to
orange-red pigments, structurally consisted of 40-carbon atom to form 8-isoprene skeletons. They are
widely distributed in nature. In 1953s, there were 30 carotenoids has been discovered. In 1971, the
number of carotenoids determined increased to 273 compounds. Until 1995, the number had reached
563 carotenoids. At the time of writing this abstract, there were 1204 natural carotenoids in 722 source
organisms that have been identified and deposited in the Carotenoid Database (see
http://carotenoiddb.jp/). Carotenoids are very interesting system. Their chemical structures determine
the light-absorption properties and chemical reactivity that form the basis of most functions. Specifics
interactions with other molecules in the immediate vicinity strongly influence the properties of a
carotenoid and are also crucial to functioning. Carotenoids play important role in the photosynthesis
reactions, i.e. to harness the sunlight energy, to transfer the excited energy to chlorophylls, and
responsible for photo-protection and as antioxidants. Its consumption can result in various health
benefits, with potential for therapeutic application. My research group study carotenoids in some most
incredible marine microbes and exotic fruits found in Indonesian territory. We study the different
composition of carotenoids in these unique species, where and in which forms the carotenoids are
found in their native molecular environment, how does the metabolic process involved with regards
to carotenoids, and also investigate their phycological functions. In this conference, I will introduce
part of our exciting findings that have been delivered by the members of my research group. These
research findings include evaluation of provitamin A carotenoids during the ripening stages of Agung
Semeru Banana (Musa paradisiaca L.), the kappa ()-end group carotenoids of Papuan Red Fruit
(Pandanus conoideus L.), and sulfur-containing carotenoids from a marine coral symbiont
Erythrobacter flavus strain KJ5.
Keywords: carotene, chromatographic separation, MS/MS analysis, xanthophyll

24
Advanced Design Photocatalyst for Waste Water Remediation by Aerosol
Process
Osi Arutanti
Research Center for Physics, Indonesian Institute of Sciences, Kawasan Puspiptek, Serpong,
Indonesia
osiarutanti@yahoo.com
Invited Speaker
ABSTRACT
Since environmental problems have become an important issue yet to be solved, the solar energy
driving photocatalysis process based on semiconductors is considered a promising technique for
wastewater remediation. The critical considerations for photocatalyst wastewater treatment remain
the same; the photocatalyst material's efficiency and efficiency of the photocatalyst synthesis process.
Here, nanostructured photocatalyst has been successfully prepared by the aerosol process, i.e., Flame
Assisted Spray Pyrolysis (FASP) and Electric Spray Pyrolysis (ESP). The result showed that this
method effectively designs visible-active photocatalyst for wastewater remediation because of their
advantages, such as continuous operation, high production rate, and controllable process. The effect
of several process parameters on the particle formation and morphology was studied and used to
decompose rhodamine B (RhB) and amaranth as the organic waste model under visible light.

25
Phytochemical Characterization and in vitro Biological evaluation of
phytochemical from Diospyros lotus
Abdur Rauf
Department of Chemistry, University of Swabi, Anbar-23561, K.P.K, Pakistan
Invited Speaker
ABSTRACT
The main aims of this study was the isolation of seven new dimeric napthoquiones namely di-
naphthodiospyrol A (1), di-naphthodiospyrol B (2), di-naphthodiospyrol C (3), di-naphthodiospyrol
D (4), di-naphthodiospyrol E (5), di-naphthodiospyrol F (6), di-naphthodiospyrol G (7) from the
chloroform soluble fraction of Diospyros lotus. The chemical structure of all isolated compounds
were elucidated by advanced spectroscopic analysis which include 1H-NMR, C13-NMR, HSQC,
HMBS, NOESY and J-resolved experiment. All the isolated compounds (1-7) were also evaluated
for their effects on the reversion of multidrug resistant (MDR) mediated by P-glycoprotein using
rhodamine- 123 exclusion screening test on human MDR1 gene transfected mouse gene transfected
L5178 and L5178Y mouse T-cell lymphoma. The compounds (1-7) were also assessed for
antiproliferative effect on L5178Y mouse T-lymphoma cell line. The tested compounds showed
promising MDR reversing effect in a dose dependent manner against mouse T-lymphoma cell line.
All isolated compounds (1-7) were also screening for urease inhibition activity which showed
excellent activity.
Key words: Diospyros lotus; phytochemicals, urease; reversion of multidrug resistant

26
ORAL PRESENTER

27
Biodegradation of DDT by Co-cultured of Pleurotus eryngii and Pseudomonas
aeruginosa
Diana Maulianawati1, Adi Setyo Purnomo2*, Ichiro Kamei3
1
Department of Aquaculture, Faculty of Fisheries and Marine Science, Universitas Borneo Tarakan, Amal
Lama No.01, Tarakan Timur, Tarakan, 77123 Indonesia
2
Department of Chemistry, Faculty of Science and Data Analytics, Institut Teknologi Sepuluh Nopember,
Kampus ITS Sukolilo, Surabaya 60111, Indonesia
3
Department of Forest and Environmental Science, Faculty of Agriculture, University of Miyazaki, 1-1,
Gakuen Kibanadai-nishi, Miyazaki 889-2192, Japan
*Corresponding author: adi_setyo@chem.its.ac.id
Oral Pesenter
ID Paper : O20200001
ABSTRACT
Dichloro-diphenyl-trichloroethane (DDT) is a synthetic insecticide that widely used around the

world, which has a negative effect on human health and the environment. The objective of this
research was to investigate the ability of bacterium Pseudomonas aeruginosa in co-culturing with
white-rot fungus Pleurotus eryngii to degrade DDT. The various volume of P. aeruginosa (1 ml ≈
1.5 x 109 CFU) were added into 10 mL of P. eryngii culture for a 7-days of incubation. Approximately
82% of degradation of DDT were obtained from co-cultures with the adjunct of 10 ml of P.
aeruginosa during the 7-day incubation period, which had the best ratio of optimization of 0.57. The
confrontational assay showed that P. aeruginosa gave no effect on the growth of P. eryngii (0.39
cm/day). DDD (1,1-dichloro-2,2-bis(4-chlorophenyl) ethane), DDE (1,1-dichloro-2,2-bis(4-
chlorophenyl) ethylene) were detected as metabolic products from the DDT degradation by co-
cultures bacterium P aeruginosa and fungus P. eryngii. This study indicated that bacterium P.
aeruginosa can be used to enhance DDT degradation by whire-rot fungus P. eryngii.
Keywords: Bioremediation; Biodegradation; Pleurotus eryngii; Pseudomonas aeruginosa; DDT.

28
Antibacterial and Toxicity Activities of Indonesian herbal medicine
extracts used for postpartum treatment
Wiwit Denny Fitriana 1 , Sri Banun Titi Istiqomah2 , Taslim Ersam 3 , Adi Setyo Purnomo 3 , Devi
Anggraini Putri3 , Nurlatifah3 and Sri Fatmawati3,a)
1
Faculty of Sciences and Technology, Universitas Pesantren Darul Ulum, Jombang 61481, Indonesia
2
Faculty of Health, Universitas Pesantren Darul Ulum, Jombang 61481, Indonesia
3
Department of Chemistry, Faculty of Science, Institut Teknologi Sepuluh Nopember, Surabaya 60111,
Indonesia
*Corresponding author e-mail: fatma@chem.its.ac.id
Tel / Fax: +62-31-5943353 / +62-31-5928314
Oral Pesenter
ABSTRACT
Postpartum treatments have been used by local women in Indonesia for some time now. One
commonly used postpartum treatment is the consumption of a traditional herbal medicine called
PHM-1 during the first 40 days after childbirth. In addition, a second medicine known as PHM-2 is
taken for days 41-80. However, sufficient scientific evidence about the benefits of this postpartum
herbal medicine does not exist. In this study, the antibacterial abilities of PHM-1 and PHM-2
therefore was evaluated and showed positive potential for both. Low IC 50 values were obtained
against pathogenic bacteria such as Escherichia coli, Staphylococcus aureus, Bacillus subtilis,
Pseudomonas aeruginosa, Rhodopseudomonas palustris, Ralstonia pickettii, and Staphylococcus
epidermidis and compared with chloramphenicol as a positive control. The antibacterial activity of
both PHM-1 and PHM-2 against R. pickettii had the highest inhibitory activity as evidenced by the
lowest IC50 values of 11.16 and 6.26 μg/mL, respectively. Furthermore, an in vivo acute toxicity test
showed that PHM-1 produced a low LD50 value of 3.28 g/kg BW for both of male and female mice.
These results support the use of both PHM-1 and PHM-2 as antibacterial treatments for postpartum
women.
Keywords: antibacterial, toxicity, postpartum treatment, Indonesian herbal, LD50

29
Polyol from Epoxidized Candlenut Oil (Aleurites Moluccanus) and Poly
Ethylene Glycol (PEG) 200 : Kinetic Study
May Kurnia Pratiwi1, a) Rochmadi1,b) and Budhijanto1, c)
1
Department of Chemical Engineering, Faculty of Engineering,
Universitas Gadjah Mada, Yogyakarta 55281, Indonesia
maykurnia96@mail.ugm.ac.id
rochmadi@ugm.ac.id
budhijanto@ugm.ac.id
Oral Pesenter
ABSTRACT
Polyol is the main raw material to produce polyurethane which is widely used in the commercial and
industrial fields. The use of candlenut oil to become polyols by epoxidation process is a new
alternative method, which has advantages: renewable raw materials and biodegradable polymer. The
purpose of this research is to study the rate of reaction of epoxidized candlenut oil with PEG 200,
together with the characteristics of the polyols produced. The candlenut oil has iodine number of
109.9 mg I 2 /g. This experiment was carried out in a three necked flask as batch reactor equipped
with magnetic stirrrer, thermometer, waterbath, reflux condenser, and heater. In the first process,
epoxidation of candlenut oil at 60 o C took 4 hours as the optimal time to produce epoxy of candlenut
oil with the oxirane number of 6.092 g/mol. The second process was polymerization of epoxidized
candlenut oil and PEG 200-based polyol at 110oC, 120oC, 130oC, and 140oC, running for 45 minutes
to 180 minutes. The results showed that reaction rate of secondary hydroxyl chains is greater than
that of primary hydroxyl. The proposed kinetic model fitted well in the early stage of reaction, before
the reaction mixture become viscous.
Keywords: candlenut oil, epoxidation, poly ethylene glycol, polymerization, polyol

30
The Beneficiacion of Sumbawa Manganese Ore using Response Surface Method
(RSM)
Alfikri Khair1, a) Haryudini Arsa Putri 2, Suprapto3, b), Yatim Lailun Ni”mah4
1-4
Department of Chemistry, Institut Teknologi Sepuluh Nopember, Surabaya, Indonesia.
Corresponding author: a) alfikrik@gmail.com
b) suprapto@chem.its.ac.id
Oral Pesenter
ABSTRACT
The beneficiation of manganese ore from Sumbawa, East Nusa Tenggara, Indonesia has been carried
out by leaching using sulfuric acid (H2SO4). The effects of sulfuric acid concentration, leaching
temperature, reaction time were optimized. The leaching parameters were set based on Box-Behnken
Design and optimized using Response Surface Methodology (RSM). The beneficiation product was
prepared for the synthesis of lithium manganese oxide (LiMn 2O4) that will be applied in lithium ion
batteriy cathode. X-Ray Flouroscense (XRF), X-Ray Diffraction (XRD) and Atomic Absoption
Spectroscopy (AAS) were performed for manganese ore and product characterization. The optimum
yield of manganese ore leaching was 79.57%. The optimum yield produced was achieved by leaching
manganese ore in sulfuric + acid 2.5 M, leaching temperature of 70 °C and leaching for 9 hour.
Keywords: Manganese, Manganese Ore, Leaching, Response Surface Method

31
Development of PVDF/LiCl Polymeric Membrane for Harvesting of
Nannochloropsis sp.
Taufik Qodar Romadiansyaha , Badrut Tamam Ibnu Alia , Widi Citra Lestaria,
Marisa Permatasaria , Dini Ermavitalinib and Nurul Widiastutia1
a
Department of Chemistry, Institut Teknologi Sepuluh Nopember, 6011 Sukolilo, Surabaya, Indonesia
b
Department of Biology, Institut Teknologi Sepuluh Nopember, 6011 Sukolilo, Surabaya, Indonesia
Oral Pesenter
ABSTRACT
One of the biggest challenges in implementing microalgae-based biofuels is the effective harvesting
process. Filtration membrane has become one of the flexible methods in microalgae harvesting. This
study investigated the harvesting of Nannochloropsis sp. using a modified PVDF membrane at
various LiCl as an additive using thermally induced phase separation (TIPS) method with N-methyl-
2-pyrrolidone (NMP) solvent. The optimum membrane composition was determined at various LiCl
additive from 1% to 3% (w/w). The prepared membrane was characterized using SEM to determine
the morphology of the membrane and FTIR to determine the structure of the membrane chemical
properties. These results revealed that hydrophilicity of the membrane increased as indicated by the
increasing of water permeation value, which were 516 L/m 2 h; 546 L/m 2 h; 660 L/m 2 h; 614/m 2
h for neat PVDF; PVDF/LiCl 1%; PVDF/LiCl 2%; PVDF/LiCl 3% membrane, respectively.
PVDF/LiCl 2% membrane showed the highest water permeation (660 L/m 2 h) and the highest
average flux 150 L/m 2 h, as well as 100% of microalgae rejection of Nannochloropsis sp harvesting.
Keywords: PVDF/LiCl membrane, Nannochloropsis sp. harvesting, TIPS, LiCl as additive

32
Application of Ionic Rectification for Water Purification: Desalination with a
Nafion Cationic Diode
Budi Riza Putra1,2, Frank Marken1
1
Department of Chemistry University of Bath Claverton Down Bath BA2 7AY United Kingdom
2 Indonesia Defense University, Kawasan IPSC Sentul, Sukahati, Kec. Citeureup, Bogor, Jawa Barat, 16810
E Mail : budiriza@gmail.com/brpp20@bath.ac.uk
Oral Pesenter
ABSTRACT
A proof-of-principle (prototype) device of desalination or salination process driven by AC-electricity

has been developed based on asymmetrically deposited Nafion ionomer onto 100 microholes array
as a cationic diode material combined with Fumasep FAS-130 as an anion conducting membrane.
The prototype utilizes a 3D-printed four-chamber system with two internal chambers to extract
and/accumulate the salt in order to demonstrate desalination or salination processes. The
demonstration of desalination or salination processes have been shown in three operational modes
(device configurations) for an aqueous 250 mM NaCl solution. It has been demonstrated the process
of 50% desalination/salination using three operational modes in a prototype device. Due to the
experimental restrictions such as (i) material resistivity lowering rectification effect, (ii) rectification
ratio limitations for the 100 microholes array, and (iii) insufficient stability of ionomer membranes
under AC-pulse-driven operation, the charge efficiency is estimated to be only 12% over 18 hours
experiment time. However, this chapter describes the new technology for desalination/salination
applications with some perspectives for the improvements in the future.
Keywords: ionic rectification, water desalination prototype, Nafion, desalination/salination process,

cyclic voltammetry

33
Analysis of Heavy Metals (Pb, Cd, Cu and Cr) with Inductively Coupled Plasma
Spectrometer (ICPS) on Cracker of Sea Cucumber (Phylloporus spiculatus) at
Kenjeran, Surabaya, Indonesia
Ririn Sumiyani1, Nurmalina Mulliani1, Catherina Ratna1, Orchidea Rachmaniah2
1
Faculty of Pharmacy, University Surabaya, Jl Tenggilis Mejoyo, Kalirungkut, Surabaya, 60294, Indonesia
2
Chemical Engineering Department, Institut Teknologi Sepuluh Nopember, Sukolilo, Surabaya, 60111,
Indonesia
orchidea@chem-eng.its.ac.id / Orchidea Rachmaniah
Oral Pesenter
ABSTRACT
Cracker of Sea Cucumber (Phylloporus spiculatus) is favorite and popular snack from Surabaya. It
generally produced by small-medium sized enterprise (Usaha Kecil Menengah/UKM) at Kenjeran,
Surabaya. Due produced by UKM, usually it has a P-IRT (Produk-Industri Rumah Tangga) code for
its production and distribution. Therefore, as it is consumed, a requirements should be fulfilled either
according to National Agency of Drug and Food Control (Badan Pengawas Obat dan
Makanan/BPOM) or World Health Organization (WHO) especially in heavy metals content.
Regulation of BPOM No. 5/2018, strictly regulates a maximum allowable limit of heavy metals of
processes foods from Crustacea and Echinodermata. They are 0.25, 0.20, and 0.10 mg/kg,
respectively for As, Pb, Hg, and Cd. While Cr and Cu is only permitted at maximum 0.5 ppm and 10
mg/day, respectively, by FAO (Food and Agriculture Organization). Samples of crackers of Sea
Cucumber were collected from 12 fishermen and Sea Cucumber processors at Kenjeran market,
Surabaya. They were randomly chosen and analyzed by ICPS. A validation method of ICPS analysis
was previously established for quantitative analysis of heavy metals; including selectivity, linearity,
limit of detection (LOD), limits of quantitation as well as accuracy, and precision. It was found that
none of samples containing Cd. However, Pb, Cu, and Cr of heavy metals were detected exceeding
the maximum allowable limit. They were 252.88 mg/kg, 34.15 mg/kg, and 1.6 ppm, respectively, for
Pb, Cu, and Cr. A counseling to the UKM producer of crackers of Sea Cucumber need to be
conducted, informing a good food processing practices. A simultaneous analysis of sea water and
other sea biota at Kenjeran Area is also necessary, knowing whether the contamination comes from
the pollution of the sea water or form the Sea Cucumber itself.
Keywords: Cracker, Contamination, Sea cucumber, Heavy metals, Kenjeran.

34
Anti-cancer Activity of Okra Polysaccharides on Human Liver Cancer Huh7it
Cells Growth and Natural Killer Cell
Suhailah Hayaza, Win Darmanto, Sri Puji Astuti Wahyuningsih, Raden Joko Kuncoroningrat
Susilo
Universitas Airlangga , Indonesia
suhailah.hayaza@gmail.com / +6281216609751
Oral Pesenter
ABSTRACT
Liver cancer or scientifically known as Hepatocellular Carcinoma (HCC) is an uncontrolled growth

of malignant cells in the liver and is one of the deathliest diseases in the world. The purpose of this
study was to determine the potential of okra raw polysaccharides extract (ORPE) as an anti-cancer
agent, based on its effect on growth and apoptosis percentage of liver cancer cells Huh7it, as well as
natural killer (NK) cell activation. ORPE extraction was carried out using absolute ethanol and water.
Huh7it cells were grown in DMEM medium and cultured 24 hours before treatments. NK cells were
isolated from male mice (Mus musculus), BALB/c. Cells were divided into 4 groups: splenocyte
control group (K), negative control group (KN), positive control group given doxorubicin dose of 10
μg/mL (KP), and ORPE (P) treatment groups. The ORPE group was divided into 5 subgroups based
on their doses: ORPE dose of 50 µg/mL (P1), dose of 100 µg/mL (P2), dose of 200 µg/mL (P3), dose
of 400 µg/mL (P4), and dose 600 µg/mL (P5). Measurement of Huh7it cell growth and NK cell
activation was carried out using the MTT method. Measurements of Huh7it cell apoptosis were
analyzed by flowcytometry. The percentage data of Huh7it cell growth and NK cell activation were
analyzed statistically with p = 0.05. The results showed that ORPEs were significantly able to inhibit
Huh7it cell growth, increase NK cell activation, and trigger apoptosis in Huh7it cells. The most
optimal anti-cancer ability shown was ORPE dose of 600 µg/mL.
Keywords: raw polysaccharides extract, apoptosis, Huh7it, natural killer cell, cell growth

35
Characteristics of Edible Film Derived from Amylose Fraction of Sago Starch
Witta Kartika Restu1, Dewi Sondari2, Teo Duri Yosta3, Anna Muawanah3
1
Polymer Chemistry Group, Research Center for Chemistry, Indonesian Institutes of Sciences (LIPI),
Kawasan Puspiptek Serpong, South Tangerang, Banten, Indonesia 15314
2
Technology Process of Biomass and Bioremediation Group, Research Center for Biomaterial, Indonesian
Institutes of Sciences (LIPI), Kawasan Cibinong Science Center, Jl. Raya Bogor Km.46, Cibinong, Bogor,
West Java, Indonesia 16911
3
Department of Chemistry, Syarif Hidayatullah State Islamic University, J Jl. Ir H. Juanda No.95, Cempaka
Putih, Ciputat, South Tangerang, Banten, Indonesia 15412
e-mail: witt001@lipi.go.id
Oral Pesenter
ABSTRACT
The present study investigated the edible film properties that derived from the fractionation process
of sago starch. Amylose plays a critical role in in term of food application. Amylose that derived
from sago starch fractionation was prepared as the material for edible film. The obtained amylose
was produced using butanol solvent in the fractionation process. Then each of them was characterized
by its thickness, morphology and contact angle. The resulted edible film from amylose has the
thickness of 0.23 mm. This thickness was in accordance with one of the requirement of materials to
become edible film. The morphology that was characterized by scanning electron microscope (SEM)
resulted in a smooth surfaces without pores or cracks. The measurement of hydrophobicity showed
the contact angle of 48.2°.
Keywords: amylose, sago starch, fractionation, edible film

36
Cracking of Jatropha Oil into Biofuel over Hierarchical Zeolite Supported
NiMo Catalyst Catalytic
Isalmi Aziz1 (U), Tri Retnaningsih1, Dhika Gustama1, Nanda Saridewi2, Lisa Adhani3
1
Department of Chemistry, Faculty of Science and Technology, State Islamic University Syarif Hidayatullah
Jakarta. Indonesia
2
Deparement of Education Chemistry, State Islamic University Syarif Hidayatullah Jakarta. Indonesia
3
Department of Engineering, Faculty of Technic, Bayangkara Univeristy, Indonesia
isalmikimia@uinjkt.ac.id
Oral Pesenter
ABSTRACT
The NiMo/hierarchical zeolite (NiMo/HZ) catalyst can used of catalytic cracking of Jatropha oil into
biofuel. Hierarchical zeolite (HZ) was synthesized using Lampung natural zeolite. Furthermore,
impregnation with various of NiMo metal (9; 1, 8: 2 and 7: 3) was carried out. The produced catalyst
was characterized by X-Ray diffraction (XRD), Surface Area Analyzer and the Temperature-
Programmed Desorption of ammonia (NH3-TPD). The catalyst activity test was carried out using a
batch of stainsless steel reactors at 375oC for 2 hours. The synthesized hierarchical zeolites show
characteristics of large surface area and acidity that are 108.5 m 2/g and 0.668 mmol/g, respectively.
The highest crystallinity of NiMo/HZ is the NiMo(7: 3)/ZH. The acidity analysis results showed that
the Ni-Mo impregnation was able to increase the acidity of the catalyst. It is figured out from the
experiment that NiMo/HZ catalysts have functioned well in the conversion of jatropha oil into
biofuels. The NiMo(7:3)/HZ catalyst resulted higher selectivity than NiMo(8:2)/HZ and
NiMo(9:1)HZ. The biofuels selectivity were 36.38% gasoline, 14.44% kerosene, and 15.99% diesel.
Keywords: Activation, desilication, natural zeolite, gasoline

37
Synthesis and Characterisation of Magnetic Nanoparticles Fe/Fe Oxide of
Sodium Borohydride Reduction Results within Chitosan Hydrogel
1
Sudirman, 1Weldan Z. Lubis, 1Mujamilah, 1Grace Tj. Sulungbudi, 2Nur Rahmayani
1
Center for Science and Technology of Advanced Materials-National Nuclear Energy Agency,
Kawasan Puspiptek Serpong, Tangerang Selatan, Banten 15314, Indonesia
2
Department of Chemistry, FMIPA-IPB
Jl. Tanjung Kampus IPB Dramaga, Bogor 16680, Indonesia
sudirmanoemar@gmail.com / 08118071258
Oral Pesenter
ABSTRACT
Nanoparticles Fe/Fe oxide coated by chitosan hydrogel have been successfully synthesized through
the diffusion process of Fe2+ ions into chitosan hydrogel followed by chemical reduction process
using sodium borohydride (NaBH4) as a reducing agent. The ratio of Fe 2+ ions to NaBH4
concentrations and chitosan hidrogel size were varied to obtain samples with a high value of
magnetization. The phase formation of chitosan, Fe and Fe oxide nanoparticles were identified by X-
Ray Diffractometer (XRD). Distribution pattern and morphology as well as the concentration of
nanoparticles formed within hydrogel were characterized by Scanning Electron Microscope-Energy
Dispersive Spectroscopy (SEM-EDS). The total amount of diffused Fe2+ ions was also measured by
atomic absorption spectrophotometer (AAS). Characterisation by Vibrating Sample Magnetometer
(VSM) indicates the formation of superparamagnetic samples with values of saturation magnetization
(Ms) maximum of 23.6 emu/g obtained at Fe: NaBH4 concentrations ratio of 1:5 and 1 mm sized of
chitosan hydrogel. This optimum condition is achieved with the total amount of diffused Fe 2+ ions
of 27.04% from its initial amount. The typical peak shift of the Schiff base observed in Fourier
transform infrared spectrophotometer (FT-IR) from 1629 cm-1 became 1174 cm-1 (C-N vibration
strain) and 1653 cm-1 (N-H secondary vibration) ensure the bond between nanoparticles Fe/Fe oxides
with chitosan hydrogel.
Keywords: Hydrogel, Chitosan, Nanoparticles, Fe/Fe oxide, NaBH4

38
Effect of Catalys and Cross Linker Concentrations on The Chemical Properties
of Sago Starch
Dewi Sondari1,a), Witta Kartika Restu2,b), Athanasia Amanda Septevani2,c), Riska Suryaningrum1,d),
Sandi Sulfiandi1,e), Rahmawati Putri1,f)
1
Research Center for Biomaterial, Indonesian Institutes of Sciences (LIPI), Kawasan Cibinong Science
Center, Jl. Raya Bogor Km.46, Cibinong, Bogor, West Java, Indonesia 16911
2
Polymer Chemistry Group, Research Center for Chemistry, Indonesian Institutes of Sciences (LIPI),
Kawasan Puspiptek Serpong, South Tangerang, Banten, Indonesia 15314
a)
Corresponding author : sondaridewi@gmail.com
b)
witt001@lipi.go.id
c)
atha001@lipi.go.id
d)
riska_suryaningrum17@yahoo.com
e)
sandi@biomaterial.lipi.go.id
f)
rahmawepe2@gmail.com
Oral Pesenter
ABSTRACT
This study was carried out to analyze the chemical characteristics of sago starch cross-linked with
sodium trimetaphosphate (STMP) and used sodium chloride as catalyst. The effect of cross linker
and catalyst (Starch/STMP/Catalyst) on swelling factor, solubility, freeze thaw stability were
investigated. The chemical properties of native and modified sago starch were comparatively studied.
Modified sago starch was produced by adding the different amounts of sodium trimetaphosphate (0.5,
1.0 and 2.0 gr) and catalyst of sodium chloride (2.0 and 4.0 gr) into reactor in water bath hotplate at
70 oC and 300 rpm for an hour. The spectra of FTIR showed new bands related to phosphorylation,
the intensity of vibration peak of –P=O bending at 1150–1414 cm-1 and P-O-C stretching at 995-
1050 cm-1 appeared in the spectra of the samples. The spectra from Fourier transform infrared (FTIR)
spectroscopy confirmed that the native sago starch was successfully modified.
Keywords: sago starch, cross link, sodium trimetaphosphate, FTIR

39
Green Synthesis of Au-Doped ZnO NANOPARTICLES (ZnO:Au NPs) by In-
situ Process using Imperatacylindrica L Leaf Extract
1
Emil Budianto, 2Iwan Syahjoko Saputra, 1Yoki Yulizar, 3Sudirman
1
Department of Chemistry, Faculty of Mathematics and Natural Sciences,
University of Indonesia, Depok 16424, Indonesia
2
Academy of Analytical Chemistry Caraka Nusantara,
Depok 16951, Indonesia
1
Center for Science and Technology of Advanced Materials-National Nuclear Energy Agency,
Kawasan Puspiptek Serpong, Tangerang Selatan, Banten 15314, Indonesia
iwan28chemistry@gmail.com / 08118071258
Oral Pesenter
ABSTRACT
ZnO:Au nanoparticles were synthesized using eco-friendly substance of Imperatacylindrica L (ICL)

leaf extract by the in-situ process. The function of Imperatacylindrica L leaf extract as a mediumfor
the formation reaction of ZnO:Au nanoparticles. Powders of ZnO:Au was analyzed using Diffuse
Reflectance Spectroscopy (UV-Vis DRS), Fourier Transform Infra-red Spectroscopy (FT-IR), X-ray
Diffraction (XRD), Scanning Electron Microscopy (SEM), Energy Dispersive X-ray (EDX) and
Raman Spectroscopy. The calculation results of UV-Vis DRS showed the value of the band gap of
ZnO changed from 3.2 eV to 2.5 eV. FT-IR showed a reduced -OH absorption peak at wavenumber
3429 cm-1, 1182 cm-1and the vibration at 502 cm-1 indicated that ZnO:Au nanoparticles successfully
synthesized. XRD showed ZnO:Au NPs crystallite size of 21.37 nm and decreasing peaks of
crystallinity in the miller index (10 0). The results of SEM-EDX showed the changes of morphology
ZnO:Au NPs from hexagonal to sphere with the composition of elements Zn, O and Au.Raman
spectroscopy showed a strong vibration peaks at 320 cm-1, 410 cm-1, 590 cm-1 and 1090 cm-1.
Keywords: Green synthesis, Nanoparticle,; ZnO:Au, Imperatacylindrica L

40
Surface Modification of Nanoporous Carbon Using Gamma Irradiation
Treatment as Supercapacitor Material
Annisa, Imam Prasetyo, Teguh Ariyanto, Deni Swantomo
Universitas Gadjah Mada, Yogyakarta
annisa96@mail.ugm.ac.id
Oral Pesenter
ABSTRACT
Nanoporous carbons, due to high specific surface area and high pore accessibility, have been utilized
as material electrode supercapacitors. To obtain high power and energy densities, it is necessary to
introduce functional groups in the carbon surface. In this research, the effect of gamma irradiation to
modify surface chemistry of nanoporous carbon synthesized from coconut shell was examined.
Alterations of surface chemistry were studied by using Fourier Transform Infrared Spectroscopy
(FTIR) and textural pore changes were determined by nitrogen sorption analysis. The results showed
that after irradiation, oxygen functional groups increased and reached optimum point at radiation
dose 25 kGy. While the specific surface area of nanoporous carbon decreased after subjected to
irradiation. Cyclic Voltammetry (CV) was used to analyze the capacitive behavior of nanoporous
carbon. CV measurements showed an improvement of specific capacitance for the irradiated
nanoporous carbon. The highest specific capacitance of 121 F/g was obtained at radiation dose 25
kGy.
Keywords: surface chemistry, nanoporous carbon, gamma irradiation, specific capacitance,

supercapacitor.

41
Improvement N2/SF6 Separation Performance on P84 Derived Carbon
Membrane by Incorporating of Zeolite-Carbon Composite
Alvin Rahmad Widyanto (ITS), Irmariza Shafitri Caralin (ITS), Nurul Widiastuti (ITS)*, Triyanda
Gunawan (ITS), Rika Wijiyanti (ITS), Wan Norhayati Wan Salleh (UTM), Ahmad Fauzi Ismail
(UTM), Mikihiro Nomura (SIT), Kohei Suzuki (SIT)
Sukolilo, Surabaya 60111, Indonesia
Advanced Membrane Technology Research Center (AMTEC), Universiti Teknologi Malaysia, 81310 UTM
Skudai, Johor Darul Ta’zim, Malaysia
Department of Applied Chemistry, Shibaura Institute of Technology, 3-7-5 Toyosu, Koto-ku, Tokyo 135-
8548, Japan
alvin16@mhs.chem.its.ac.id; irmariza16@mhs.chem.its.ac.id; nurul_widiastuti@chem.its.ac.id; triyanda@its.ac.id;
wijiyantirika@gmail.com; w-norharyati@utm.my; afauzi@utm.my; Lscathy@shibaura-it.ac.jp; mc18021@shibaura-
it.ac.jp
Oral Pesenter
ABSTRACT
SF6 gas is one of the greenhouse gasses which contributed to the global warming effect. Separation
of SF6 and N2 was investigated on the P84 and P84/ZCC carbon membrane. Carbon membranes were
prepared via pyrolysis process at 700°C under N2 atmosphere. All of the carbon membranes were
characterized by X-Ray Diffraction (XRD) and Scanning Electron Microscopy (SEM). XRD results
show a typical peak of carbon membranes at 2θ~24° () and 41-42° which correspond to the
amorphous and aromatic graphite structures, respectively. The scanning electron microscopy image
observed that the addition of ZCC filler has been successfully incorporated without producing surface
defect on the carbon membrane. The introduction of zeolite-carbon composite particles into the co-
polyimide matrix enhanced the selectivity of N2/SF6 by 136.85%. The permeability of N2 has
improved by 7.65%. Yet the permeability of SF6 has decreased by 54.55%, which indicated the
addition of ZCC filler gives a molecular sieving effect. It concluded that the P84/ZCC carbon
membrane shows better N2/SF6 separation performance than the pristine P84 carbon membrane.
Keywords: Carbon membrane, Gas Separation, Zeolite-Carbon Composite, N2/SF6, Permeability.

42
Effects of Temperature and Neutralizing Agent on Lactic Acid Production by
Rhizopus sp. Fermentation
Whiny Hardiyati Erlianab, Tri Widjajaa*, Tectona Indahc, Daryl Susilod and Annisa Meilinda Dewie
Department of Chemical Engineering, Institut Teknologi Sepuluh Nopember Surabaya, Indonesia
a)
Corresponding author: triw@chem-eng-its.ac.id
b)
whinyrliana@gmail.com
c)
indahtectona@gmail.com
d)
darylsusilo@gmail.com
Oral Pesenter
ID Paper: O20200026
ABSTRACT
Lactic acid is a raw material for Poly Lactic Acid (PLA) as a biodegradable polymer. This study
aims to investigate the effects of temperature and neutralizing agent on lactic acid production. One
of the lignocellulosic biomass which has the potential as a feedstock to produce lactic acid is sugar
palm starch waste (SPSW). SPSW has high cellulose content (40.10%) and lignin (27.24%). The
presence of lignin content can inhibit the process of cellulose hydrolysis into reducing sugar.
Therefore, the pretreatment process is necessary to remove lignin—the acid-organosolv pretreatment
using H2SO4 0.2 M and 30% ethanol with 3% NaOH as a catalyst can reduce lignin content to 27%.
Subsequently, The hydrolysis process using the cellulase enzyme from Trichoderma reseei for 24 h
at 60 oC and 125 rpm. The concentration of reducing sugar (5.3144 g/L) was analyzed using the DNS
method. Afterward, the aerobic fermentation process has carried out using Rhizopus oryzae and
Rhizopus arrhizus with various temperatures (30, 34, and 42 oC) and CaCO3 concentrations as
neutralizing agents (0.6 and 0.8 (w/v)). The highest lactic acid concentration was obtained after
incubation during 48 h at 150 rpm is 2,1751 g/L for Rhizopus oryzae and 2,7062 g/L for Rhizopus
arrhizus.
Keywords: Acid-Organosolv, fermentation, hydrolysis, rhizopus sp., waste.

43
Synthesis and Characterization of ZnO Nanoparticles Using Pumpkin Seed
Extract (Cucurbita moschata) by the Sol-Gel Method
Nanda Saridewi1*, Heri Tamtowi Syaputro2, Isalmi Aziz3, Dasumiati4, Biaunik Niski Kumila5
UIN Syarif Hidayatullah Jakarta Jl. Ir. H. Juanda 95, Ciputat, Banten, Indonesia 15412
* Corresponding author : nanda.saridewi@uinjkt.ac.id
Oral Pesenter
ID Paper: O20200027
ABSTRACT
Pumpkin (Cucurbita moschata) is one of the easiest plants to grow in Indonesia. So far, pumpkin
seeds have only been thrown away and become part of organic waste. Pumpkin seed extract has a
functional group that can function as a reducing agent, stabilizer, and capping agent in the synthesis
of nanoparticles ZnO by the sol-gel method. This study aims to test the ability of pumpkin seed
extract in synthesizing ZnO nanoparticles using the sol-gel method. This research phase begins with
the pumpkin seeds being dried and made into flour. Pumpkin seed flour was extracted and reacted
with Zn(CH3COO2. 2H2O) as a precursor with variations in the concentration of the precursors, i.e.
0.05; 0.1; and 0.15 M at Ph 8 with the addition of 0.1 M NaOH solution. The extract of pumpkin
seeds was characterized using the Fourier Transform-Infrared (FTIR) instrument. ZnO nanoparticles
were characterized using X-ray Diffraction (XRD) and Transmission Electron Microscope (TEM)
instruments. FTIR results from pumpkin seed extract showed that there are hydroxy and carboxyl
groups which allow them to act as reducing and stabilizing agents as well as capping agents in the
synthesis process of ZnO nanoparticles. XRD results showed good crystallinity for each of the ZnO
nanoparticles with a hexagonal crystal system. TEM morphology results identified that the
nanoparticles obtained had a spherical shape and had a relatively uniform particle size distribution
with an average particle size of 28.07 nm.
Keywords: ZnO, pumpkin seed, crystallinity, nanoparticle, sol-gel

44
Chemical Characteristic of Fly Ash from 3 Kalimantan’s Power Plants as
Potential Source for Synthesis of Aluminosilicate-Based Material
Rendy Muhamad Iqbal1*, Elda Alyatikah1, Erwin Prasetya Toepak1, Rasidah1, Lilis Rosmainar1,
Siswo1, and Hamzah Fansuri2
1
Department of Chemistry, Faculty of Mathematic and Natural Science, Universitas Palangka Raya,
Kampus UPR Tunjung Nyaho, Palangka Raya 73111, Indonesia
2
Department of Chemistry, Faculty of Science and Data Analytic, Institut Teknologi Sepuluh Nopember,
Kampus ITS Sukolilo, Surabaya 60111, Indonesia
*
Corresponding author: iqbal.rm@mipa.upr.ac.id
Oral Pesenter
ABSTRACT
Efforts to fulfill the needs of electricity in Indonesia are still dominated by the use of fossil fuels such
as coal in Steam Power Plants. However, the process of burning coal causes various environmental
problems, one of which is fly ash. Fly ash is classified as a dangerous and toxic material. Although
it is regarded as hazardous material, fly ash contains silica (SiO2) and alumina (Al2O3) which can be
used as raw material for synthesis of aluminosilicate-based materials such as geopolymers, zeolites
and others. In this study, the chemical composition, SiO 2/Al2O3 ratio, and fly ash type will be
examined from three different sources of fly ash such as power plants owned by a private company
located in the Barito area (FA1), Asam-Asam's (FA2), and Pulang Pisau's (FA3) power plant. The
chemical composition was tested by ASTM D 3682-13. Based on data, FA1 has the highest SiO2 and
Al2O3 composition with a percentage of 56.44% and 31.31%, respectively. FA2 has the highest Fe 2O3
composition with a percentage of 23.10% and FA3 has the highest CaO composition with a
percentage of 23.46%. The highest SiO2/Al2O3 ratio is 4.61 from FA2, while the lowest SiO2/Al2O3
ratio is 1.80 from FA1. Based on the chemical composition of fly ash, FA1 and FA2 are classified as
F type, while FA3 is classified as C type. From these data, it can be concluded that the fly ash from
the three power plants still have the potential to be used as source for synthesis of aluminosilicate-
based materials.
Keywords: aluminosilicate, fly ash, geopolymer, power plants

45
Optimization Topical Cream of Nanoemulsion Bromelan from Pineapple Core
as Antiinflamation and Antibacterial of Propionbacterium Acnes with In Vitro
Testing in Animals Test
Denny Juli Pasaribu, Sumi Hudiyono, S. Setiasih
Universitas indonesia , Indonesia
Dennyjuli.dj@gmail.com / 082258837701
Oral Pesenter
ABSTRACT
Pineapple (Ananas Comosus) contains many nutrients, especially bioactive compounds known as
bromelain. This enzyme is commonly found in pineapple core. The use of bromelain has been widely
proven in the medical field as anti-inflammatory, antibacterial, antitumor, cardiovascular treatment
and many others. In this research, the benefits of bromelain as anti-inflammatory and antibacterial
are implemented as nanoemulsion for the purpose of topical use. With a small droplet size it is able
to penetrate the surface of the skin, with the result that the intended use can be achieved. The method
of this research is high homogenizer which is one of the methods of making nanoemulsion formula.
There are three nanoemulsion formulas, all of these formulas have characteristics and stability that
fulfill the nanoemulsion standards. Nevertheless, amongst of the three formulas, the third formula is
better because it has a smaller droplet size of 22.04 nm with the viscosity of all of them being in the
gel range of 12000cps. The proteolytic activity of enzymes in this formula shows the reduction of
activity that is not significant compared to formula one and two.
Keywords: Bromelain, Nanoemulsion, Anti-inflammatory, Antibacterial

46
Irradiation Grafting of Hydrophilic Monomer onto Chitin
Gatot Trimulyadi Rekso
Center for Research and Development of Isotopes and Radiation Technology - National Nuclear Energy
Agency Jl . Lebakbulus Raya No 49, Jakarta 12240, INDONESIA
Fax 62 21 7691607, E-Mail : Gatot2811@yahoo.com
Oral Pesenter
ABSTRACT
Studies on radiation grafting of a hydrophilic monomer onto chitin for ion exchange applications
were examined. Over the past several years, chitin has been receiving increased attention for its
use, such as ion exchange. They are utilized scarcely because of problems associated with poor
solubility, hydrophilicity, and low reactivity. Graft copolymerization induced by irradiation is
one of the methods for chitin modification to improve its properties such as hydrophilicity and
reactivity. This research aims to increase the ability of chitin as ion exchange for the heavy metal
adsorbent. The hydrophilic monomers used were acrylic acid and acrylamide. In the present
experiment, the pre-radiation peroxidized method was employed. The irradiation of the sample
was carried out in the Co-60 gamma irradiation source at room temperature. The monomers
solutions acrylic acid or acrylamide than introduce into irradiated chitin, and the graft
polymerization was carried out in a nitrogen atmosphere. The presentation of grafting has been
determined as a variation of monomer concentration and temperature as a function of time of
reaction. The grafted chitin obtained was washed and dried in a vacuum, and the percentage of
grafting was measured by the gravimetric method. Measuring the capacity of adsorption of
grafted chitin to Cr, Cu, and Hg metal ion was determined by using AAS and CV AAS. The
result shows that using acrylamide gives a percentage of grafting higher compare with acrylic
acid. The FTIR spectrum demonstrates the presence of monomer grafted onto chitin with the
appearance of the carbonyl functional group. Chitin grafted with acrylic acid gives a higher rate
of adsorption of metal ion Cr, Cu, and Hg compare grafted with acrylamide. The high rate of
adsorption is for metal ion Hg. It was found 294.3 mg/g for chit-g-Aac and 257.3 mg/g for Chit-
g-Aam.
Keywords: Chitin, irradiation, grafting, ion exchange

47
Mineral, nutrition and phytochemical of ethnomedicine of Parkia Roxburghii
G.Don
Lailatul Badriyaha, Atmira Sariwatia*, Venty Suryantib, Desi Suci Handayanib, Sri Fatmawatic, Adi
Setyo Purnomoc
a
Institut Ilmu Kesehatan Bhakti Wiyata Kediri
b
Universitas Sebelas Maret
c
Institut Teknologi Sepuluh Nopember
E Mail* : atmirasari@gmail.com
Oral Pesenter
ABSTRACT
Indonesia is a country that has very rich natural resources, so that these various plants can be used in
daily life. One of them is Parkia Roxburghii G.Don, which is believed to be rich in benefits,
especially in health. Mineral needs in the body must be measured in order to fulfill comsumption in
the body to be precise. So, analysis of the mineral contents of the Parkia Roxburghii G. Don plant
was carried out on the leaves, stems and seeds. Proximate and photochemical analysis respectively
to determine nutrition and the qualitative content of antioxidants. The method used spectrometer of
XRF to determine the minerals. The results showed that the mineral conten of leaves, stems and seeds
were Ca, K, Fe, P, Mn, Re, Cu, Ba, Zn. The highest mineral content of leaves, stems and seeds was
Ca respectively 51,7; 73,9; 45,2 mg/100 g of sample. The results of phytochemicals was positively
contain tannins, saponin, flavonoids, terpenoids,
Keywords: Parkia Roxburghii, Mineral, Nutrition and phytochemical

48
Active Methylene Variations on 3-Substituted Coumarin Synthesis
Q R Tristiyana1, D U C Rahayu1*, and H Dianhar2
1
Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Indonesia, Depok
16424, Indonesia
2
Chemistry Study Program, Faculty of Mathematics and Natural Sciences, Universitas Negeri Jakarta,
Jakarta 13220, Indonesia
E-Mail: dyahutamicr@sci.ui.ac.id
Oral Pesenter
ABSTRACT
Coumarin derivatives are commonly used as antibacterial agents, especially those related to diarrhea
and acute respiratory infections. These infections drive researchers to find coumarine-based
antibacterial agents as the most widely used in therapeutic drugs worldwide. In this study, various
active methylene was used to synthesis 3-substituted coumarin following Knoevenagel condensation
under microwave-assisted condition. The highest yield of 3-substituted coumarin synthesis, 3-acetyl
coumarin (1), was achieved using ethyl acetoacetate (43.65 + 0.50% yield) in the optimum condition
of 1.2:1 ratio mol of salicylaldehyde/ethyl acetoacetate and 30 mol% of diethyl amine using 100W
microwave irradiation for 60s under solvent-free condition. Moreover, ethyl 3-coumarin carboxylate
(2, 16.33% yield) was successfully synthesized under optimum conditions of 1 by replacing active
methylene to diethyl malonate and 900s irradiation time. These two compounds were elucidated
using FTIR, UV-Vis, and NMR spectral data. However, 3-cyano coumarin (3) failed to be
synthesized using ethyl cyanoacetate and malononitrile as active methylenes under optimum
conditions of 1. This finding reveals that ethyl acetoacetate is the most reactive methylene among
others.
Keywords: activemethylene, 3-substituted coumarin, Knoevangel, microwave-assisted,

antibacterial.

49
Simulation of Modified Sorghum Flour using Lactobacillus Plantarum Bacteria
Setiyo Gunawan*, Laura Mahdalena, Ardita Azhari
Department of Chemical Engineering, Institut Teknologi Sepuluh Nopember, Surabaya. Indonesia
*Corresponding Author’s Email : gunawan@chem-eng.its.ac.id/ Telp: +62-31-5946240/Fax: +62-31-5999282
Oral Pesenter
ABSTRACT
The value of wheat imports always increases every year, this makes Indonesia a country that is
dependent on wheat imports. Efforts to reduce this dependency include the production of MOSOF
(Modified Sorghum Flour). One ingredient that can be used as a mosof making material is sorghum
(Sorghum bicolor L. Moench). Sorghum has great potential to be developed in Indonesia because it
is quite tolerant of infertile or critical soils so that less productive land can be planted. However, the
use of this plant is still very limited. In this research, sorghum is used as a substitute for wheat flour.
The stages in this research are data processing using 2 methods, namely the curve fitting sigmaplot
method and the 4th order Runge Kutta method, simulate the data obtained into the Super Pro Designer
9.5 program with variable values of α to get the optimum lactic acid yield. And if the results obtained
from the simulation are close to the data obtained, then the process of making modified sorghum
flour can begin. Validation results obtained using simulation show output that is close to experimental
data, where Haldane's kinetic model gives the best R 2 for 0.999811 by using the 4th order Runge
Kutta method and result parameter value 𝜇max, Ks, and Ki are respectively 0.8743 / hour, 0.0349 g
/L, and 6.7114 g/L. At a value of 𝜶 is 0.00008 get the optimum yield of lactic acid and lactic acid
obtained at 96 hours was 0.00779 g/L.
Keywords: Fermentation, Haldane, Lactobacillus Plantarum, Sorghum, Superpro Designer

50
Synthesis of Vanillyl Alcohol Derivatives
Reni Rahayu1, Mardi Santoso*1
1
Department of Chemistry, Institut Teknologi Sepuluh Nopember, Surabaya, Indonesia
*Corresponding author: tsv09@yahoo.com
Oral Pesenter
ABSTRACT
Eugenol (1) was oxidized with nitrobenzene to produce vanillin (2) after that vanillin (2) was reduced
to give vaillyl alcohol (5). Synthesis of vanillyl alcohol (5) derivatives, 4-(isopropoxymethyl)-2-
methoxyphenol (9), 4-(isopropoxymethyl)-2-methoxyphenyl isobutyrate (11a) and synthesis of 4-
(isopropoxymethyl)-2-methoxyphenyl butyrate (11b) is reported here. (9) was prepared from
etherification vanillyl alcohol (5) and substituted acyl halides groups gave in a good yield and purity,
and was characterized by spectroscopy methods (NMR, GC-MS. IR)..
Keywords: Vanillin, Vanillyl alcohol, Synthesis, Esterification, Identification

51
Phenolic Compounds from The Rhizomes of Indonesian Mango Ginger
(Curcuma amada)
M Amalia1, D U C Rahayu1*, H Dianhar2, and P Sugita3
1
16424, Indonesia
2
3
Department of Chemistry, Faculty of Mathematics and Natural Sciences, IPB University, Dramaga Bogor
16680, Indonesia
Oral Pesenter
ID Paper: O20200037
ABSTRACT
The rhizomes of Curcuma amada (Zingiberaceae), locally known as Temu Mangga (Mango Ginger),
are usually consumed as herbal medicine since it has been reported to show antibacterial, anticancer,
antihyperglycemic, and antitubercular activities. Many reports had also been explored for its
terpenoid contains, but the information of phenolic compounds from C. amada is still limited. This
study aims to isolate phenolic compounds from the ethanol extract of mango ginger rhizomes grown
in Bogor, Indonesia. C. amada rhizomes were macerated in ethanol to give brownish-black crude
extract (5.084%, m/m). The phytochemical screening test to the crude extract exhibited the presence
of flavonoid, alkaloid, terpenoid, tannin, and saponin. The crude extract then was fractionated and
purified using sequence of chromatographic techniques to afford two isolated fractions, C1 and F1.
According to FTIR, UV-Vis, and LC-MS/MS spectral data, the C1 fraction was identified as
curcumin, while the F1 fraction was suggested to be naringenin. This study reveals scientific
information on phenolic compounds from Indonesian C. amada rhizomes.
Keywords: Curcuma amada, curcumin, mango ginger, naringenin, and antibacterial.

52
Adsorption of Carbon Dioxide in Porous Carbon Containing
Monoehtanolamine (MEA) : The Effect of Carbon Surface Pretreatment
Xena Callista1,a, Imam Prasetyo1,b, Teguh Ariyanto1,c
1
The Carbon Material Research Group, Department of Chemical Engineering, Faculty of Engineering,
a
xenacallista@mail.ugm.ac.id, bimam.prasetyo@ugm.ac.id, cteguh.ariyanto@ugm.ac.id
Oral Pesenter
ABSTRACT
This research aims to investigate the effect of pretreatment of carbon surface oxidation in the CO 2
adsorption. First, porous carbon was oxidized using 10% H2O2 at 30, 50, and 70oC. The porous
carbon, which was oxidized or without oxidation, was impregnated using 5% monoethanolamine
(MEA). Adsorption of CO2 was carried out with a pressure up to 1 atm at 30oC. The material was
characterized by Scanning Electron Microscopy (SEM), Fourier Transform Infrared Spectroscopy
(FTIR), and N2-sorption analysis. The results showed that the surface oxidation increases oxygen
functional groups, but it decreases the surface area of porous carbon. Interestingly, the oxidized
carbon (oxidation at 30 and 50oC) exhibited an increase in the performance of the CO2 adsorption
capacity up to 34% with respect to pristine carbon. When MEA was introduced to the oxidized
carbon, it further increased the CO2 uptake. The highest adsorption capacity of 2.88 mmol CO2/gram
material was achieved.
Keywords: Adsorption, Carbon dioxide, Monoethanolamine, Oxidation, Porous carbon

53
Adsorption of Lithium and Calcium-Containing Solution for Seperation
Application
Dwi Amalia1,a, Indra Perdana1,b, Chandra Wahyu Purnomo1,c
1
a
dwiamalia@mail.ugm.ac.id, biperdana@ugm.ac.id, cchandra.purnomo@ugm.ac.id
Oral Pesenter
ABSTRACT
Lithium is currently one of the most needed elements due to its main role in lithium-ion batteries
(LIBs) and its scarce availability in the world. Spent LIBs become a potential source of lithium,
especially for countries with no resource of lithium in nature. It is necessary to develop an efficient
lithium recovery process which can produce lithium compound for further reuse. Lithium can be
recovered from LIBs cathode through a hydrometallurgical process, which requires sequential
precipitations of components. Calcium is frequently added to the precipitation process, which at the
end might disturb the purification of lithium compound products. This work studied the purification
of lithium from calcium-containing solution by adsorption. The solution might resemble the leaching
solution of spent LIBs cathode. The artificial solution containing 2000 ppm lithium and 500 ppm
calcium were adsorbed using 0.5 grams of cationic resin for 24 hours at 30, 50 and 70°C, respectively.
Experimental results showed that calcium has a higher adsorption affinity against lithium. The
adsorption at 30°C was dominated by calcium intake of 81.07%, while lithium was only 18.67%.
This result indicates that the adsorption method using cationic resins can be used to selectively
separate lithium from the calcium-containing solution, which is advantageous for further application
in LIBs recycling.
Keywords: Adsorption, Calcium, Lithium-ion battery, Purification, Resin

54
Optimization Study of Alkyl Polyglycoside C12 Synthesis using Indirect Method
F P A Kinanti1, D U C Rahayu1*, A P Gustianthy2, and Y K Krisnandi1
1
16424, Indonesia
2
PT Pertamina Research and Technology Center, Jl. Raya Bekasi KM 20, Pulogadung, Jakarta 13920,
Indonesia
Oral Pesenter
ABSTRACT
Alkyl polyglycosides (APGs) are classified as non-ionic and green surfactants that can be synthesized
from renewable materials such as glucose and fatty alcohol. APGs are commonly applied as
surfactants in enhancing oil recovery since APGs exhibit excellent interfacial activities, emulsified
abilities, foaming performance, and wettabilities. In this research, the mixture of APG C12 was
synthesized following the indirect method, which was consisted of two stages, i.e. butanolysis and
transacetalization. Butanolysis was carried out by reacting glucose technical grade with 1-butanol
(1:2.8, m/m) and PTSA catalyst 1 mol% at 106 oC for 6h. The mixture of APG C4 then was further
applied in transacetalization without purification process by adding 1-dodecanol with some
optimizations, i.e. mass ratio of 1-dodecanol, reaction time, and temperature. The optimum condition
to afford the mixture of APG C12 was the mass ratio of glucose and 1-dodecanol 1:7.5 m/m with
PTSA catalyst 1 mol% at 115oC for 4h. All the mixture of APGs were analyzed for its remaining
reducing sugar level using Benedict’s test and further characterized using FTIR, UV-Vis, and LC-
MS/MS spectral data. Moreover, physical characteristic tests such as compatibility, emulsion
stability, solubility, and interfacial tension tests were also conducted to all the mixture of APGs. This
study reveals that APGs can be synthesized using the indirect method with moderate to good physical
characteristics which can be used for enhancing oil recovery as further application.
Keywords: alkyl polyglycoside, non-ionic, green surfactant, indirect method, and enhanced oil
recovery.

55
Physiogenetik And Root Architecture of Tobacco Plants (Nicotiana tabacum)
Under Periodic Waterlogging Stress
Tutik Nurhidayati, Hery Purnobasuki, Sucipto Hariyanto,
Vita Siti Fatimah, Zulfan Febriawan
1
(Tutik Nurhidayati, Department of Biology, Institut Teknologi Sepuluh Nopember, Surabaya, Indonesia, Jl.
Arief Rahman Hakim Kampus ITS Surabaya 60111, Indonesia) btutikn72@gmail.com
2
(Hery Purnobasuki, Departement of Biology, Faculty of Science and Technology, Airlangga University.
Surabaya, East Java 60115. Indonesia, Indonesia).ahery-p@fst.unair.ac.id
2
(Sucipto Hariyanto, Departement of Biology, Faculty of Science and Technology, Airlangga University.
Surabaya, East Java 60115. Indonesia, Indonesia).asucipto-h@fst.unair.ac.id
1
(Vita Siti Fatimah, Department of Biology, Institut Teknologi Sepuluh Nopember, Surabaya, Indonesia, Jl.
Arief Rahman Hakim Kampus ITS Surabaya 60111, Indonesia), ereeivita@gmail.com
1
(Zulfan Febriawan, Department of Biology, Institut Teknologi Sepuluh Nopember, Surabaya, Indonesia, Jl.
Arief Rahman Hakim Kampus ITS Surabaya 60111, Indonesia), ezfebriawan@gmail.com
a)
Corresponding author: hery-p@fst.unair.ac.id
Oral Pesenter
ABSTRACT
Periodic waterlogging stress is the limiting factor of growth of tobacco. This study aims to investigate the
response of physiology, molecular and architectural roots of some varieties of tobacco plants (Nicotiana
tabacum) on a periodic waterlogging stress. The method used in this study is a periodic waterlogging stress
first stage to be continued waterlogging stress second stage (partial submergence) for each 12 days. Indicators
tobacco experiencing stress waterlogging of periodic review of aspects of physiology such as chlorophyll
content, productivity, the ratio of the canopy: roots and Net Assimilation Rate (NAR), as well as on the genetic
aspects include the expression of genes responsive to stress waterlogging periodically include genes Lateral
Root primordium 1 (LRP1) and genes Xyloglucan Endotransglycosylase / Hydrolase (XTH) as well as rooting
architecture. Rooting architectural aspects include morphology in the root zone. The results showed that
periodic waterlogging stress reduced tobacco productivity by 50-70%, chlorophyll content by 42-48%, and
Net Assimilation Rate (NAR) by 76.97 - 109.4%, and increased canopy: root ratio by 2.7- 6. Varieties Jepon
Emas has the best response to the parameters of chlorophyll content with the lowest decrease of 42.15%, and
NAR of 76.97%, as well as an increase in the ratio of canopy: the lowest root of 2.78, while on productivity
parameters, Jinten varieties have the best response with the lowest decrease of 50.23%. The Prancak 95 variety
is the most sensitive variety under periodic waterlogging stress conditions. The results showed that periodic
waterlogging stress reduced tobacco productivity by 50-70%, chlorophyll content by 42-48%, and Net
Assimilation Rate (NAR) by 76.97 - 109.4%, and increased canopy: root ratio by 2.7- 6. Varieties Jepon Emas
has the best response to the parameters of chlorophyll content with the lowest decrease of 42.15%, and NAR
of 76.97%, as well as an increase in the ratio of canopy: the lowest root of 2.78, while on productivity
parameters, Jinten varieties have the best response with the lowest decrease of 50.23%. The Prancak 95 variety
is the most sensitive variety under periodic waterlogging stress conditions. From the genetic aspects,
responsive gene expression Lateral Root primordium 1 (LRP1) and gene Xyloglucan Endotransglycosylase /
Hydrolase (XTH) were up-regulated under waterlogging stress treatment for all varieties. On Architecture
Prancak 95 varieties rooting showed the highest decline of 15% -78% on the parameters of the number of root,
root diameter, root fraction horizontal, IJA and ICA. Jinten highest varieties decreased 15% in root length.
Varieties Jepon highest decreased 35% fraction of vertical root and experienced the highest increase of 27% -
35% on adventitious roots and a shoot-root ratio.
Keywords: Nicotiana tabacum, Physiogenetic, Periodic, Root Architecture,Waterlogging Stress

56
Influence of Binders on Properties of Biomomass Briquettes from Durian peel
(Durio kutejensis Becc)
Andi Dulung Wirabuana, Ratna Surya Alwi
Fajar University, Indonesia
ratnasya@gmail.com
Oral Pesenter
ABSTRACT
Briquettes are widely used as renewable energy to solve the problems of excessive use of wood as
fuel. However, the performance of briquettes depends on the types of pyrolysis, including the nature
of binders used during the preparation. Their performances are related problems such as low yield
and energy content that are significantly improved with the use of adhesives in the right compositions.
In this work, we investigated the effect of binders compositions on the quality of briquettes from the
pyrolysis of Durian lai peel (Durio kutejensis Becc). The briquettes were prepared from Durian peel
charcoal by adding starch as a binder at various concentrations of 3%, 4%,5%, and 6% (w/w).
Subsequently, analysis of moisture content, volatile matter ash content, and calorific value were
performed. It was found that the best quality obtained was binder concentration of 3 % (w/w).
Keywords: Briquiettes, Binders, Durian peel, Charcoal, Pyrolysis.

57
Influence of Particle size of Lai peel (Durio kutejensis Becc) charcoal on
Properties of Biomomass Briquettes
Andi Dulung Wirabuana, Ratna Surya Alwi
ratnasya@gmail.com
Oral Pesenter
ABSTRACT
Briquettes are widely used as renewable materials to energy generation due to their excellent energy
obtained. However, the performance of briquettes depends on the types of pyrolysis, including the
particle size of charcoal used during the preparation. Their performances are related problems such
as low yield and energy content. Therefore, the knowledge particle size of charcoal is essential to
develop biomass briquettes. In this study, we investigated the effect of particle size of charcoal on
the quality of briquettes from the pyrolysis of Durian lai peel (Durio kutejensis Becc). The briquettes
were prepared from Durian peel charcoal at a various particle sizes of 0.25 mm, 0.425 mm, 1.70 mm,
and 2.00 mm. Subsequently, analysis of moisture content, volatile matter, ash content, and calorific
value were performed. It was found that the best quality of briquette obtained was particle size of
2.00 mm.
Keywords: Briquettes, Particle size, Durian peel, Charcoal, Pyrolysis.

58
Synthesis of Hemiterpene 1-Bromo-3-Methyl-2-Butene Derivatives
Devi Anggraini Putri, Mardi Santoso
Department of Chemistry, Faculty of Sciences, Institut Teknologi Sepuluh Nopember, Indonesia
devianggrainiputri@gmail.com / tsv09@yahoo.com
Oral Pesenter
ABSTRACT
Hemiterpene 1-bromo-2-methyl-2-butene (prenyl bromide) is widely used as a prenyl source for

organic synthesis. One of them is the development of synthetic fragrances for the perfume industry
which is growing rapidly for daily consumption. Based on our literature study, most fragrances
contain of terpenes. There are two common groups used as fragrances such as oxygenated
monoterpenes and sesquiterpenes. Interestingly, hemiterpenes are potential but they are rarely
isolated from natural products. In this research, three hemiterpene derivatives have been successfully
synthesized from prenyl bromide and obtained in good yields. Furthermore, the synthesis method
was conducted by two steps. The first step was prepared by acid-base reaction, followed by
nucleophilic substitution of 1-bromo-3-methyl-2-butene. The hemiterpene derivative structures were
confirmed by NMR and mass spectroscopy.
Keywords: hemiterpene, fragrance, prenyl bromide

59
Interpenetrated Polymer Network of Bacterial Cellulose
Nanang Masruchin1,2,*, Adelia Hajar Diastirini3, Fazhar Akbar1, Arif Nuryawan4,*
1
Research Center for Biomaterials, Indonesian Institute of Sciences (LIPI), Jl Raya Jakarta Bogor Km 46
Cibinong, 16911, Indonesia
2
Pusat Kajian Nanoselulosa, Indonesian Institute of Sciences (LIPI), Jl Raya Jakarta Bogor KM 46
Cibinong, 16911, Indonesia
3
Polymer Chemistry Department, Polytechnic STMI Jakarta, Ministry of Industrial, Republic of Indonesia
4
Department of Forest Products Technology, Faculty of Forestry, Universitas Sumatera Utara, Jl. Tri
Dharma Ujung No. 1 Kampus USU Medan, 20155, North Sumatera, Indonesia
E Mail : (Nanang Masruchin : masruchin@biomaterial.lipi.go.id) (Arif Nuryawan : arif5@usu.ac.id)
Oral Pesenter
ABSTRACT
This study was aimed to obtain strong and lightweight material from pristine nanocellulose of
bacterial cellulose (BC) which produced from Acetobacter xylinum bacteria. The nanocellulose
hydrogel resulted from inter- and intrafibrillar hydrogen bonding cellulose was used as the first
network. While the second network was formed by immersion of vacuum and oven dried of BC in a
mixture of citric acid and glycerol in 1 to 1 ratio for 2, 4, 6 and 24 h followed by crosslinking initiation
by heating at 130 oC for 15 h. The composite film was characterized for mechanical testing, infrared
spectroscopy and microscopy analysis. The result showed that vacuum dried BC film obtained high
strength compared to oven dried BC film which suggested that slow water evaporation minimize
fibrils hornification and effectively facilitated the penetration of glycerol and citric acid. The
optimum strength was obtained at 6 h immersion indicated that the higher second network loading
resulted in brittle material. Infra red spectra showed that ester linked was formed which corresponded
to the formation of interpenetrated polymer network.
Keywords: bacterial cellulose, IPN, hydrogen bonding, ester linked, lightweight material

60
Biodiesel Synthesis from Waste Cooking Oil and using CaO derived from green
conch shell waste and limestone as Catalyst by Microwave
Budiani Fitria Endrawati 1 , Niar Kurnia Julianti 2 , Azmia Rizka Nafisah 2 , Chandra Suryani
Rahendaputri 3 , Endah Mutiara 4
1
Departement of Industrial Engineering, Institute Technology of Kalimantan Balikpapan, Indonesia
2
Departement of Chemical Engineering, Institute Technology of Kalimantan Balikpapan, Indonesia
3
Department of Enviromental Engineering, Institute Technology of Kalimantan Balikpapan, Indonesia
4
Department of Chemistry, Institute Teknology of Sepuluh Nopember Surabaya, Indonesia
a) Corresponding author: <wati.bdi@gmail.com>
b) emmputri@gmail.com
Oral Pesenter
ABSTRACT
Biodiesel is well known in the industry that is a substitution of diesel oil in the transportation sector.
Crude palm oil is mostly used for biodiesel production. However, the price of crude palm oil is
fluctuate that cause high cost of biodiesel production. On the other hand, restaurants produce an
abundance waste cooking oil and has cheaper price than crude palm oil. The objective of this study
is reduce free fatty acid from waste cooking oil by microwave assisted esterification with low reaction
power and its application for biodiesel. Although conventional method is have less efective energy
transfer and longer reaction time to get high yield and conversion. Therefore, we used a microwave
assisted esterification to lower free fatty acid value by sulfuric acid. Biodiesel synthesis using CaO
derived from from green conch shell waste and limestone (1:1 w/w) as heterogenous catalyst by
microvawe assisted transesterification. The experimental results indicated that the best performance
esterification is 0,0241 mg NaOH/g of acid value by reaction time of 15 minute at microwave power
400 W. The highest biodiesel yield (83,89%) was found by metanol oil ratio 1:12 at microwave power
of 400 W and reaction time of 15 minute.

61
Modification of Rubber Seeds (Hevea brasiliensis) as Adsorbent to Improve The
Quality of Used Fried Cooking Oil
Gatut Ari Wardani, Melfa Ristia, Rosmaya Dewi
Department of Pharmacy Bakti Tunas Husada Health Science College E
Corresponding author: gatutariwardani@stikes-bth.ac.id
Oral Presenter
ID paper : O20200047
ABSTRACT
Cooking oil is one of the basic needs of the Indonesian people to meet their daily needs. Repeated
use of cooking oil by heating at high temperatures can affect the quality of cooking oil so that when
consumed, it is not suitable for your health. One of the methods used to improve the quality of used
cooking oil (waste cooking) is by using NaOH2% activated rubber seed adsorbent. This study aims
to determine the quality of rubber seed shell adsorbents and the ability of rubber seed shell adsorbents
to improve the quality of used cooking oil such as organoleptic properties, free fatty acid content,
and water content and evaporated material. The results of this study indicate that the variation of the
adsorbent activation time is 0.5; 12; 24 and 48 hours obtained the optimum activation time at 0.5
hours. Variation of adsorbent contact time for used cooking oil that is 30, 60, 90, and 120 minutes
cannot improve organoleptic properties such as color and odor. Still, it can reduce the value of free
fatty acid levels with an optimum time of 30 minutes with a percent reduction reaching 55.21% and
reduce the amount of water content and evaporating material with an optimum time of 120 minutes
with a percent decrease reaching 73.33%.
Keywords: adsorbent, rubber seed shell, activation, used cooking oil

62
Effect of pH, Temperature, and Electrolytes on Swelling and Release Behaviors
of PVA/AAM/GO Based Hydrogel Composities
Fitri Khoerunnisa1*, Mita Nurhayati1, Rimadina Nurul Hikmah1, Hendrawan1, Fitri Dara2, Yaya
Sonjaya1, Muhammad Nasir2, Hafiz Aji Aziz1
1Department of Chemistry, Indonesia University of Education, Bandung 40154 Indonesia
2Research Unit for Clean Technology, Indonesian Institute of Sciences, Bandung 40135 Indonesia
Corresponding Author: fitri@upi.edu
Oral Presenter
ABSTRACT
Hydrogels have been used in variety of applications, ranging from tissue engineering to drug delivery
as controlled release material. In this study, the effect of pH, temperature, and electrolytes on swelling
and release behavior of PVA/AAM/GO based hydrogel composites (HC) has been carefully
investigated. The HC film were successfully prepared by solution mixing method at optimum volume
ratio of PVA/AAM/GO of 5:10:4, respectively. The structural morphology characterization of HC
was carried out by means of FTIR spectroscopy, X-ray diffraction, and scanning electron microscopy
(SEM).In particular, the effect of environmental conditions on the swelling behavior of HC was
performed on the variation of pH (2, 7 and 9), NaCl concentration (% w/w) (1%, 4%, 10%, 25% and
28%), and temperature (4 °C, 26 °C and 40 °C). The FTIR spectra confirmed the chemical interaction
between PVA, AAm, and GO in HC matrix that predominantly occur through hydrogen bonding.
The insertion of GO into polymer matrices notably modified the morphological structure of
superabsorbent hydrogel composites, as ascertained by FTIR, SEM images and XRD patterns. The
environmental condition remarkably affected the swelling behavior of HC, where the swelling
behavior notably increased at pH 9 and temperature of 40°C conditions. The addition of NaCl also
significantly enhanced the swelling capacity of HC up to 560% at optimum concentration of 25%.
This result inferred that HC exhibited a great sensitivity toward pH, temperature, and electrolyte
concentration.
Keywords: Hydrogel composites, swelling behavior, PVA, AAM, GO

63
Characterization of Mineral Content from Puntaru Beach Iron Sand
Martasiana Karbeka, Faryda V. Lamma Koly, Nini Mau Tellu
Tribuana Kalabahi University, Indonesia
Corresponding author: martasianakarbeka@untribkalabahi.ac.id
Oral presenter
ABSTRACT
Natural iron sand is a magnetic material that has considerable potential as a source of iron ore
(Fe). The presence of iron sand in nature is mixed with other non-magnetic material so that the
sample preparation is done. The iron sand samples obtained were extracted using an external
magnet to separate magnetic and non magnetic materials. Extracted magnetic material is crushed
then washed with distilled water. Iron sand samples that have been washed with distilled water
are characterized by X-Ray Fluorescence (XRF) knowing the types of elements contained in iron
sand, Fourier Transform Infrared (FT-IR) Spectrophotometer and to identify functional groups
present in iron sand and X-Ray Diffraction (XRD). The results of the characterization of Puntaru
iron sand using X-Ray Fluorescence (XRF) showed that the puntaru iron sand was dominated by
Fe (77.80%), Ti (8.51%), Al (5.47%) and Si (3.76%) ) and several other minor elements. The
highest percentage of Fe element was confirmed as iron oxide in the form of Fe3O4 (magnetite).
The results of the analysis of magnetic properties show that Puntaru's iron sand is soft magnetic.
Keyword: iron sand, puntaru beach, magnetite, preparation, mineral oxides

64
New QSPR Study for Predicting Critical Micelle Concentration of Gemini
Cationic Surfactants Using the Online Chemical Modeling Environment
(OCHEM)
Ely Setiawan 1,2, 3, a) , Karna Wijaya1 and Mudasir Mudasir1
1
Department of Chemistry, Universitas Gadjah Mada, Yogyakarta, 55281, Indonesia
2
Austrian-Indonesian Centre for Computational Chemistry (AIC), Department of Chemistry, Universitas
Gadjah Mada, Yogyakarta, 55281, Indonesia
3
Department of Chemistry, Universitas Jenderal Soedirman, Purwokerto, 53122, Indonesia
a)
Corresponding author: ely.setiawan@mail.ugm.ac.id
Oral Presenter
ABSTRACT
In this paper, the relation between the critical micelle concentration (cmc) and the molecular
structure of 231 gemini cationic surfactants with the various hydrophilic head group has been
developed using a quantitative structure-property relationship (QSPR) method. The QSPR models
were developed using the Online CHEmical Modeling environment (OCHEM) platform. It
provides several machine learning methods and molecular descriptors sets as a tool to build QSPR
models. The molecular descriptors were calculated by eight different software packages including
Dragon v6, OEstate and ALogPS, CDK, ISIDA Fragment, ChemAxon, Inductive Descriptor,
Mordred, and PyDescriptor. A total of 64 QSPR models were generated with 6 top-ranked models.
Based on the statistical coefficient of QSPR models, the model 5 which is constructed from
combination of ASNN method and Mordred descriptors, provided the best QSPR models. The
model 5 performed the highest predictive result with R 2 = 0.95, q2 = 0.95, RMSE = 0.17, and
MAE = 0.11. The model was freely available at https://ochem.eu/model/25147470 and can be
used for estimation of cmc of new gemini cationic surfactants compound at the early steps of
gemini cationic surfactants development.
Keywords: critical micelle concentration, gemini cationic surfactant, machine learning, OCHEM,
QSPR

65
The Effect of Amine Types on Breakthrough Time for CO2 Gas Seperation
Performance
Kuni Masruroh, Rochim Bakti Cahyono, Imam Prasetyo, Teguh Ariyanto
Gadjah Mada University, Indonesia
kunimasruroh@mail.ugm.ac.id, rochimbakti@ugm.ac.id, imampras@ugm.ac.id, teguh.ariyanto@ugm.ac.id
Oral Presenter
ABSTRACT
Methane (CH4) and carbon dioxide (CO2) are the main components of a renewable energy source of
biogas. Separation of CO2 from biogas is significantly important to improve biogas performance, due
to heating value in biogas depends on the concentration of methane. One of the gas separation
technologies that has been widely used in chemical industries is carbon molecular sieve (CMS). This
research explores the potential of CMS for biogas purification. CMS was prepared by modification
of palm kernel shell-derived porous carbon using amine groups such as monoethanolamine (MEA),
2-amino-2-methyl-1-propanol (AMP), and diethanolamine (DEA). The CMS was characterized
using N2 sorption analysis, fourier transform infrared spectroscopy, thermogravimetric analysis, and
scanning electron microscopy. The effect of amine types on the separation parameters was studied
using a breakthrough experiment. The results showed that among the three types of amines, CMS
impregnated with MEA had the highest performance for CO2 gas separation.
Keywords: amine groups, biogas, breakthrough time, carbon molecular sieve, CO2 gas separation

66
Differential Pulse Voltammetry Study for Quantitative Determination of
Disporsium (III) in Acetonitril Solution
Santhy Wyantuti *, Uji Pratomo, Shauvina Ayutias Asyifadewi, Yeni Wahyuni Hartati, Husein
Hernandi Bahti
Department of Chemistry, Faculty of Mathematics and Natural Sciences, Padjadjaran University. Jl. Raya
Bandung Sumedang Km 21. Jatinangor, Sumedang 45363. Tel / Fax 022-7794391
* email: santhy.wyantuti@unpad.ac.id
Oral Presenter
ABSTRACT
Dysprosium has gained global interest due to the main application of modern technology. The
presence of this rare earth element (REE) can be determined by several spectroscopic methods.
Recently, a voltammetry method provides an alternative method for simple and fast detection of REE.
However, to the best of our knowledge, the experiment was usually carried out in an aqueous solvent.
Therefore, we curious to study the response of REE in an organic solvent by the voltammetry method.
In this research, we conduct a study for quantitative detection by differential pulse voltammetry of
dysprosium and its mixture with samarium, europium, and gadolinium in acetonitrile. A Box-
Behnken design was applied to predict the optimum condition of the measurements. Three factors,
namely potential deposition, deposition time, and amplitude modulation were found to have the
optimal conditions of -1.0 V, 83.64 seconds, and 0.0929 V, respectively. Our study shows the
detection limit value as 0.6462 mg/L and the quantitation limit 2.1419 mg/L with the precision value
99.97%.
Keywords: Acetonitrile, Box-Behnken design, Dysprosium, Differential Pulse Voltammetry

67
Phytochemical and Antioxidant Activities of Robusta Coffee Leaves Extracts
from Alor Island, East Nusa Tenggara
Rosalina Y. Kurang, Rinda Kamengon
Departmen of Chemistry, Faculty of Math and Science, Universitas Tribuana Kalabahi, Indonesia
Corresponding author: rosalinayuliana89@gmail.com
Oral Presenter
ABSTRACT
Coffee is a very promising commodity. Besides being consumed as a famous drink with high caffeine
content, coffee also has many compounds that can act as antioxidants. One part of the coffee plant
that acts as an antioxidant is the leaf. Robusta coffee (Coffea canephora) is one species of the genus
Coffee. The aims of this study were to determined the phytochemical content and antioxidant activity
of Robusta coffee leaves from Alor Island, East Nusa Tenggara. Robusta coffee leaves were extracted
by maceration for 24 hours with methanol. The extracts were evaluated for their phytochemical and
antioxidant activity. DPPH assay was used to determine the antioxidant activity. The result showed
that Robusta coffee leaves from Alor Island gave the antioxidant activity with IC50 56,337 ppm.
Phytochemical analysis revealed that the bioactive compound from Robusta coffee leaves is
composed of alkaloids, flavonoids, terpenoids, and phenolic.
Keywords: antioxidant, DPPH assay, phytochemical, robusta coffee

68
Bioluminescence Study of Red Dragon Fruit (Hylocereus polyrhizus) Extract in
Various Solvents
Yehezkiel Steven Kurniawan1, Edi Setiyono1, Muhammad Riza Ghulam Fahmi1, Hendrik Oktendy
Lintang1,2, and Leny Yuliati1,2*
1
Ma Chung Research Center for Photosynthetic Pigments,
2
Department of Chemistry, Faculty of Science and Technology, Universitas Ma Chung, Indonesia
E-mail: leny.yuliati@machung.ac.id
Oral Presenter
ABSTRACT
Special attention has been given to red dragon fruit (Hylocereus polyrhizus) because it contains
unique natural products and exhibits wide biological activities. However, their bioluminescence
properties and their potential use as photonic material have not been addressed yet. In the present
work, simple extraction of red dragon fruit was carried out to study the bioluminescence properties
of the corresponding extracts in various solvents. At first, the flesh and peel of red dragon fruit were
separated, dried, and macerated for 24 hours using distilled water, ethanol, and acetone, separately.
The results demonstrated that the red dragon fruit exhibited different luminescence properties
depending on the fruit section and the used solvent. It was revealed that the ethanolic extract of red
dragon fruit either of flesh or peel gave the highest fluorescence intensity among the others. The flesh
of the red dragon fruit extract showed two excitation peaks at 228 and 293 nm, yielding only a single
emission peak at 335 nm when monitored at both excitation wavelengths. Meanwhile, the peel of red
dragon fruit extract showed two excitation peaks at 290 and 359 nm, yielding different emission
properties. Excitation at 290 nm gave one emission peak at 339 nm, while the excitation at 359 nm
gave two emission peaks at 436 and 671 nm. Such strong bioluminescence properties observed in a
wide range of UV and visible regions demonstrated the potential use of the red dragon fruit extract
as a photonic material.
Keywords: Bioluminescence, Fluorescence, Maceration, Red Dragon Fruit, Solvent

69
Detection of Triethylamine on Supramolecular 3-[(E)-(4-
Acetylphenyl)Diazenyl]-4-Hydroxybenzaldehyde Compound
Leny Yuliati1,2*, Tiffany Meyrista Diandra Ismahar Nazella1, and Krisfian Tata Aneka Priyangga2
1
Ma Chung Research Center for Photosynthetic Pigments,
2
Department of Chemistry, Faculty of Science and Technology,
Universitas Ma Chung, Indonesia
Corresponding author: leny.yuliati@machung.ac.id
Oral Presenter
ABSTRACT
Triethylamine (TEA) has been reported to cause irritations that occurred in the system of respiratory,
ocular, and dermal tissues. Therefore, the development of materials that able to detect the TEA is
required. In this work, we reported the synthesis of a new supramolecular azo compound for potential
detection of TEA in solution, which was 3-[(E)-(4-acetylphenyl)diazenyl]-4-hydroxybenzaldehyde.
The successful formation of the azo compound was supported by the thin layer chromatography
(TLC) and Fourier transform infrared spectroscopy (FTIR). The optical and fluorescence properties
of the compound were examined by diffuse reflectance ultraviolet-visible (DR UV-vis)
spectrophotometer and spectrofluorometer. DR UV-vis showed the wide light absorption from UV
to visible region up to ca. 600 nm. The excitation and emission spectra revealed that the azo
compound could be excited at 237 and 280 nm as the main excitation wavelengths, in which both
excitation wavelengths resulted in a single emission peak at 312 nm. The detection of the TEA was
examined by monitoring the changes in the emission intensity before and after the addition of TEA.
The higher the amount of added TEA, the lower the emission intensity at 312 nm. The quenched
emission intensity was more observed at the excitation wavelength of 237 nm than 280 nm,
suggesting that the TEA would favor the interactions with the azo compound at the excitation sites
of 237 nm. This study demonstrated the potential detection of TEA on the supramolecular azo
compound.
Keywords: Azo compound, Detection, Fluorescence, Supramolecular, Triethylamine

70
Synthesis of Solid and Hollow TiO2 Nanofibers with Electrospinning Method
Hadi Nugraha Cipta Dharma1,c), Safiratul Firdaus1, d), Nurul Widiastuti1, a), Juhana Jaafar2, b)
1
Institut Teknologi Sepuluh Nopember, Indonesia
2
Universiti Teknologi Malaysia, Malaysia
a)
Corresponding author: nurul_widiastuti@chem.its.ac.id
b)
juhana@petroleum.utm.my
c)
cipta16@mhs.chem.its.ac.id
d)
safiratul16@mhs.chem.its.ac.id
Oral Presenter
ABSTRACT
Owing to its high stability and non-toxicity, TiO2 has gained immense attention as photocatalyst
candidate for organic pollutant degradation through photogenerated radicals. As promising
nanostructure, TiO2 nanofibers was found to be interesting with several advantages such as
flexibility in its surface function, high mechanical stability and very large surface area to volume
ration toward the light irradiation. In this study, solid and hollow TiO2 nanofibers were
synthesized using electrospinning method. The obtained solid TiO2 nanofibers were characterized
using scanning electron microscope – energy dispersive X-ray (SEM-EDX) that revealed
morphological form with average diameter of (331.6 + 148.4) nm and elemental composition of
titanium (Ti), oxygen (O) and carbon (C) by 61.65%, 29.08% and 9.27%. Meanwhile, SEM result
also shown nanofibers form of polymeric template for hollow TiO 2 nanofibers with average
diameter of (460.1 + 72.3) nm. Moreover, obtained hollow TiO2 nanofibers have similar result
from previous study.
Keywords: degradation, photocatalyst, TiO2, nanofibers, electrospinning

71
Hepatoprotective Effect of Crude Extract Polysaccharide Ganoderma
applanatum on Carbon Tetrachloride-induced Liver Fibrosis in Mice
Raden Joko Kuncoroningrat Susilo (U), Suhailah Hayaza, Saikhu Akhmad Husen, Dwi Winarni,
Sri Pudji Astuti, Win Darmanto
Department of Biology, Faculty of Science and Technology, Universitas Airlangga, Surabaya 60115,
Indonesia
Corresponding author: jokokuncorosusilo@gmail.com
Oral Presenter
ABSTRACT
Liver fibrosis is a treatable liver disease. Ganoderma applanatum is a fungus known for alternative
medicine. Previous studies have reported that G. applanatum can act as an antioxidant, anti-
inflammatory, and anticancer. This study aimed to evaluate the hepatoprotective effect of crude
polysaccharide extract G. applanatum (CEPG) on liver fibrosis due to carbon tetrachloride (CCl 4)
induction. BALB/c mice were given CCl4 intraperitoneally (2 mL/kg; 1: 3) for eight times in four
weeks to produced a hepatic fibrosis model and were divided into five groups along with a control
group (water only). Silymarin (100 mg/kg) and CEPG (25, 50, and 100 mg/ kg) were given daily for
four weeks and 5 hours after CCl4 administration. Biochemical markers on serum and liver
homogenate for measurement of ALT, AST, laminin, HA, TNF-α, and IL-6 levels were determined
by kit. The administration of CCl4 significantly increased ALT and AST levels. Likewise with the
levels of laminin, HA, TNF-α, and IL-6. Otherwise, the CEPG treatment group was able to reduce
these hepatic disease parameters. The hepatoprotective effect of CEPG on liver fibrosis may be due
to its anti-inflammatory effects. CEPG can be a potential hepatoprotective agent for later clinical
therapy.
Keywords: Anti-inflammatory, Carbon tetrachloride, Crude extract polysaccharides, Fibrosis,

Ganoderma applanatum

72
Microencapsulation of Gadolinium Citrate Using Silica for Contrast Agent
Development and Its Encapsulation Efficiency Determination
Amalia Septiani, Anni Anggraeni, Ari Hardianto, Diana Rakhmawaty Eddy
Department of Chemistry, Universitas Padjadjaran , Indonesia
Corresponding author: diana.rahmawati@unpad.ac.id
Oral Presenter
ABSTRACT
Gadolinium is one of rare earth elements. In its forms of coordination compounds, gadolinium is
widely used as a diagnostic contrast agent because it provides a better image for Magnetic Resonance
Imaging (MRI). However, toxic gadolinium ions can be released from the coordination compounds
and poison the human body. This problem can be solved through the encapsulation of gadolinium
coordination compounds, such as Gd-citrate, using silica. Silica encapsulation offers a solution to use
Gd-citrate as a safe MRI contrast agent. Since the resulting silica-encapsulated Gd-citrate is a colloid,
therefore, the encapsulation efficiency should be determined by using the standard addition method
to minimize errors in matrix, not the external standard. This study aimed to prepare silica-
encapsulated gadolinium citrate (Gd-C6H5O7@SiO2) and determine the encapsulation efficiency by
using the standard addition method. Gadolinium citrate was encapsulated using the Stӧber sol-gel
method, by mixing Gd-citrate, ethanol, aqua injection, TEOS, and ammonia. Particle size analysis
using PSA provides information on the average size of Gd-C6H5O7@SiO2 of 1.53 µm. The result of
visible light spectrophotometry with the standard addition method showed a high encapsulation
efficiency value of 90.44%.
Keywords: Gadolinium, MRI, microcapsule, silicon dioxide (SiO2), standard addition.

73
Synthesis and Characterization of Ion Imprinted Polymer for Selective
Separation of Cd(II)
R. Y. Pahlawan, D. A. Nurania), I. Abdullah, and R. Wibowo
Department of Chemistry, Faculty of Mathematics and Natural Sciences (FMIPA), Universitas Indonesia,
a)
Corresponding author: d.arifa@sci.ui.ac.id
Oral Presenter
ABSTRACT
Cadmium become one of toxic heavy metals that can be dangerous for human lives. At very low
concentration, cadmium cannot be determined by instrument easily. Ion Imprinted Polymer (IIP) is
used to recognize and separating heavy metal ion that works with trapping method in polymer
matrices that are crosslinked with chelating agent. In this research, IIP via bulk polymerization with
cadmium ion as template, 8-Hydroxyquinoline as ligand, methyl methacrylate as functional
monomer, EGDMA as Crosslinker, and AIBN as Initiator was successfully synthesized. The effect
of ratio of ligand and monomer (1:1, 1:2, 1:3) to IIP was studied. The synthesized IIP was
characterized by AAS, FTIR, SEM, and TGA. Adsorption capacity of IIP reach optimum capacity at
pH 6 with 60 minutes contact time. IIP with ratio 1:2 has the adsorption mechanism of both IIP and
NIP followed to the Freundlich equation with R2 0.9214 and maximum adsorption capacity of Cd(II)
was 335.58 mg/g for IIP and 105.46 mg/g for NIP respectively. Furthermore the kinetics model
followed the pseudo first order with R2 0.9722, this results show that the adsorption mechanism took
place on initial time of adsorption. IIP exhibited good selectivity to Cd 2+ ions in the presence of Pb2+
and Cr3+ interferences.
Keywords: Ion Imprinted Polymer, 8-Hydroxyquinoline, Cd(II) ion, Polymerization, and Adsorption

74
Zeta Potential Examination of β-Carotene Encapsulated In Starch-
Chitosan/Tripoly-phosphate Microparticles
Agnes Dyah Novitasari Lestari1,2*, Dwi Siswanta2, Ronny Martien3, Mudasir Mudasir2*
1
Department of Chemistry, Universitas Papua, Manokwari, Indonesia
2
Department of Chemistry, Universitas Gadjah Mada, Yogyakarta, Indonesia
3
Department of Pharmacy, Universitas Gadjah Mada, Yogyakarta, Indonesia
Corresponding author: mudasir@ugm.ac.id, a.dyahnovitasari@gmail.com / phone: +6281328891911
Oral Presenter
ABSTRACT
This study examined zeta potential of some encapsulation products of β-carotene in the starch-
chitosan/TPP matrices. the effects of weight ratio of starch to chitosan, β-carotene loading, and
tripolyphosphate (TPP) addition level on zeta potential of the microparticle products were
determined. The native and hydrolyzed starches as well as low molecular weight chitosan were used
in the preparation of microparticles. The synthesis of microparticle was carried out by dropwise
addition of ethanolic dispersion of β-carotene into the starch-chitosan/TPP aqueous mixture. The
results showed that zeta potentials of all synthesized microparticles are between 5.8-13.2 mV with a
negative charge, except for microparticle with the weight ratio of starch to chitosan of 4:16. The
increase in β-carotene addition level from 10 to 20 mg did not change zeta potential charge. From
the variation of TPP addition in the range of 400-600 mg, it was found that the highest colloidal
stability was shown by TPP addition of 600 mg for the composition of weight ratio of starch to
chitosan of 10:10 and β-carotene addition of 10 mg. These results also confirm that the encapsulation
products exhibit colloidal instability in water.
Keywords: starch, β-carotene, chitosan, encapsulation, zeta potential

75
Response Surface Methodology of Calcite Precipitation using Sodium
Lignosulfonate and Polyaluminium Chloride
Muhammad Ihsan Ibnu Adi, Suryo Purwono, Muhammad Mufti Azis
Gadjah Mada University, Yogyakarta, Indonesia
Corresponding author: muhammadihsan95@mail.ugm.ac.id, spurwono@ugm.ac.id, muhammad.azis@ugm.ac.id
Oral Presenter
ABSTRACT
Lignin is a potential natural resource and can be converted into various value-added products. Lignin
based flocculant, sodium lignosulfonate (SLS) and polyaluminium chloride (PAC) as the coagulant
were used to treat wastewater containing calcite suspension. This study aimed to optimize the
flocculation process using both SLS and PAC by the response surface methodology (RSM). The
experiments were carried out using jar test for a constant medium stirring speed and coagulant dosage
by varying pH and flocculant dosage. A second order model was proposed based on a central
composite design (CCD) with single response of settling time. The analysis result showed that the
optimum condition was achieved for 15.53 min of settling time using 6.91 and 0.42 g/L for pH and
flocculant dosage, respectively. At optimum condition, a settling time of 16.65 min or 7.18% from
the RSM analysis was obtained. A validation experiment indicates that the result was closed to the
predicted value, which shown that the proposed model can be used to describe well the flocculation
process.
Keywords: Flocculation, Optimization, Wastewater, Sodium lignosulfonate, Response surface

methodology

76
The Effect of Stannous Chloride Catalyst in the Formation of Pla from DL-
Lactic Acid as an Eco-Friendly Plastic Raw Material
Liza Saharani Hamzah, Akbar Hanif Dawam, Juliandri
Department of Chemistry, Universitas Padjadjaran , Indonesia
Corresponding author: juliandri@unpad.ac.id
Oral Presenter
ABSTRACT
Poly Lactic Acid (PLA) is a type of plastic made from renewable biomass that can be used like
conventional plastics, but can degraded by the activity of microorganisms into water and carbon
dioxide gas so that it is eco-friendly, and can reduce the accumulation of plastic waste. This study
was conducted to determine the effect of stannous chloride catalyst in the PLA production from a
racemic mixture of DL-lactic acid using a simple azeotropic condensation method. PLA synthesis
was carried out by adding catalyst concentration variation 0.01, 0.015, and 0.02% (w/w). The
molecular mass calculation of the polymer were performed using the Ostwald viscometer, the results
obtained were 15740, 17783, and 24044 g/mol. PLA characteristics are determined by Fourier
Transform Infrared Spectroscopy (FTIR), Thermogravimetric Analysis (TGA), and Differential
Scanning Calorimetry (DSC). After comparing with standard PLA, FTIR spectra results were
identified as typical peaks of PLA. The results of TGA characterization showed that the PLA obtained
by adding a catalyst of 0.02% (w/w) had the highest thermal stability. The DSC results obtained for
the PLA melting point values are 100, 102.8, and 103.2⁰C. Based on this research, the concentration
of stannous chloride catalyst directly affects the increase in molecular mass which determines the
physical properties of PLA bioplastics.
Key words: azeotropic condensation, catalyst, DL-lactic acid, poly lactic acid, stannous chloride

77
Methylene Blue Removal from Waste Water Using Sodium Lignosulfonate and
Polyalumunium Chloride: Optimization With RSM
Lutfa Rahmawati, Muhammad Mufti Azis, Rochmadi
Department of Chemical Engineering, Faculty of Engineering, Gadjah Mada University, Indonesia
Corresponding author: lutfarahmawati@mail.ugm.ac.id, muhammad.azis@ugm.ac.id, rochmadi@ugm.ac.id
Oral Presenter
ABSTRACT
The growth of textile industry is accompanied by the increase of wastewater containing synthetic
dyes. Without proper treatment, synthetic dyes may possess environmental burden. Here, removal of
methylene blue as the model compound of synthetis dye has been investigated with the aid of
flocculants. Combination of two types of flocculants: Sodium Lignosulfonat (SLS) and
Polyalumunium Chloride (PAC) was optimized for methylene blue removal using Response Surface
Methodology (RSM). We have implemented a centered composite design (CCD) with 3 replicates
with single response of methylene blue removal efficiency. The experiments were conducted with
jar test units and methylene blue removal was estimated using UV-Vis spectrophotometer. The result
showed that the maximum of color removal could be achieved at SLS 0.773 g and PAC 1 g, from
which the color removal was 99.7% and the minimum of color removal was 38.7% at SLS 0.5 g and
PAC 1.5 g. The analysis also confirmed that the color removal improved by increasing SLS dosage.
Keywords: Surfactants, Flocculation, Methylene blue, RSM, Jar test

78
The Effect of Precursors on Synthesis of TiO2 and Their Photocatalytic Activity
on Degradation of Phenol
Muhamad Diki Permana, Atiek Rostika Noviyanti, Diana Rakhmawaty Eddy
Department of Chemistry, Universitas Padjadjaran, Indonesia
Corresponding author: diana.rahmawati@unpad.ac.id
Oral Presenter
ABSTRACT
Photocatalysis can degrade phenols into other compounds that are relatively safer for the
environment. One of the most widely developed photocatalysis materials is titanium dioxide
(titania/TiO2). TiO2 is widely used because it has high catalytic activity properties, is stable and the
price is relatively cheap. Tetrabutyl orthotitanate (TBOT) and tetraisopropoyl orthotitanate (TTIP)
are the most commonly used precursors of TiO2. These differences in precursor can cause the phase
difference of TiO2 crystals that are formed which further influences its nature in the activity of
photocatalysis. In this study, TiO2 was synthesized from a variety of precursors, TBOT, and TTIP
through the sol-gel method and calcination temperatures of 800 ℃. The physicochemical properties
of the synthesized product were studied using X-Ray Diffraction (XRD) and UV-Vis
Spectrophotometer. The synthesized TiO2 was applied to degrade phenol under UV irradiation. TiO2
which was synthesized from TBOT and TTIP precursors had higher crystallinity and lower bandgap
than P25 Degussa.TiO2 from TBOT precursors has more anatase structure, higher bandgap energy,
and has a higher percentage of phenol degradation than TiO2 from TTIP precursor, which was
82.38% for TiO2 from TBOT precursor and 56.14% for TiO2 from TTIP precursor after UV
irradiation for 2 hours.
Keywords: phenol, photocatalyst, TiO2, TBOT, TTIP

79
Molten Salt Synthesis of Photocatalyst Material SrTix-1FexO3 (x= 0, 0.05, 0.1,
0.15, and 0.2)
Aldi Dwi Prasetiyo, Dwi Rahayu Novianti, Hasal Maulidianingtiyas, Anton Prasetyo*
Department of Chemistry, Faculty of Science and Technology, Universitas Islam Negeri Maulana Malik
Ibrahim Malang, Jalan Gajayana 50 Malang, 65144 Indonesia.
Corresponding author: anton@kim.uin-malang.ac.id
Oral Presenter
ABSTRACT
SrTiO3 is a perovskite-type structure material which received many attention because it has a wide
potential in various fields such as piezoelectric and photocatalyst. As photocatalyst material, the band
gap energy of SrTiO3 is 3.2 eV as results will works in the UV light wavelength. Doping metal to
SrTiO3 was reported to decreases its band gap energy. In this work, we synthesized SrTix-1FexO3 (x=
0; 0.05, 0.1, 0.15, and 0.2) using molten salt method. Diffractogram of SrTix-1FexO3 (x= 0, 0.05, 0.1,
0.15, and 0.2) showed that the samples were successfully synthesized, but at x= 0,2 have small
impurities of TiO2 phase. SEM images showed that the morphology of particles is cubic and iron
dopant may cause agglomeration. UV-Vis DRS spectra showed that iron dopant can reveal the light
absorption in visible wavelength region.
Keywords: photocatalyst, strontium titanat, iron dopant, molten salt synthesis, visible light
absorption

80
Multianalytical characterizations for investigating the origin of prehistoric rock
art pigments of Karim Cave, Sangkulirang, East Kalimantan, Indonesia
M. M Ilmi, N. Nurdini, E. Maryanti, C. Saiyasombat, P. Setiawan, G. T. M. Kadja, Ismunandar
a
Division of Inorganic and Physical Chemistry, Institut Teknologi Bandung, Jl. Ganesha 10, Bandung
40132, Indonesia
b
Synchrotron Light Research Institute, 111 University Ave., Muang, Nakhon Ratchasima 30000, Thailand
c
Division of Visual Communication and Multimedia, Institut Teknologi Bandung, Jl. Ganesha 10, Bandung
40132, Indonesia
d
Research Center for Nanosciences and Nanotechnology, Institut Teknologi Bandung, Jl. Ganesha 10,
Bandung 40132, Indonesia
Corresponding author: $kadja@chem.itb.ac.id, *ismu@chem.itb.ac.id
Oral Presenter
ABSTRACT
Sangkulirang, East Kalimantan is a home of one of the oldest rock art in the world. Among more
than fifty caves, Karim cave comprises three distinctive pigments colors, i.e., purple, dark red, and
red pigments. This study employed a series of multianalytical characterizations to investigate the
physicochemical properties of the pigments in order to reveal the information about the origin of
pigment preparation during prehistoric era. The SR-XRD (Synchrotron Radiation-X-ray Diffraction)
pattern of all pigments exhibited that hematite (Fe 2O3) serves as major content of the pigments. Also,
based on the Scherer crystallite size calculation, the purple pigment exhibited largest crystallite size
followed by dark red, and red pigments. Based on XRF (X-ray Fluorescence) analysis, purple
pigment comprises the highest hematite content. Furthermore, the Fe K-edge XANES (X-ray
Absorption Near-Edge Spectroscopy) was utilized to reveal the discrepancy of the electronic
transition properties of the pigments. It shows that the edge energy (E 0) of all pigments are located
at 7126.6 eV, showing the content of Fe3+ iron species. Interestingly, the deconvolution of pre-edge
spectra exhibited the difference of absorption ratio of t 2g/eg due to the presence of the distortion of
octahedral coordination in hematite crystal. The centroid peak analysis confirmed that the purple
pigment exhibited highest octahedral distortion. As conclusion, the geometrical distortion is playing
a major role in generating the different colors of the pigments. These discrepancies are likely to be
originated from different heating treatment prior to the application of the pigments. It can be inferred
that the purple pigment was prepared by a higher temperature and/or longer heating treatment
followed by dark red and red pigments.
Keywords: Rock art, Karim cave, hematite, synchrotron, XANES

81
Characteristics of Fish Protein Concentrate Extracted from Snakehead fish
(Channa striata) using Two Different Extraction Methods
Diah Ikasari1) and Mohamad Indra Nata Wijaya2)
1)
Research Center for Marine and Fisheries Product Processing and Biotechnology,
KS Tubun Petamburan VI Jakarta Pusat 10260
2)
Padjajaran University, Jl. Raya Bandung Sumedang KM.21, Hegarmanah, Jatinangor, Kabupaten
Sumedang, Jawa Barat 45363
Corresponding author: ikadiah263@gmail.com
Oral Presenter
ABSTRACT
The present study aimed to investigate the characteristics of fish protein concentrate extracted from
Snakehead fish (Channa striata) using two different methods, namely heating and combination
methods. Using heating method, the cooked minced meat was mixed with distilled water and heated
at 80ºC prior centrifugation, drying and grinding into powder, while in the combination method the
minced meat was treated with combination techniques between washing and acidification. The
protein concentrates were analyzed for parameters of yield, colour, proximate value and functional
properties, including water holding capacity, water absorption capacity, fat absorption capacity,
activity and stability of emulsion. Results showed that yield of protein concentrate produced from
combination and heating methods were 21% and 27%, respectively. Combination method produced
fish protein concentrate with higher values of moisture and protein contents compared to heating
method, while it remains low for ash and fat contents. Both methods resulted in FPC with
insignificant different in color, however, FPC produced by combination method demonstrated higher
ability to hold and absorb water which is desirable for food formulation.
Keywords: snakehead fish, fish protein concentrate, heating method, combination method,
extraction.

82
Introducing Closed Form Solution of Riccati Differential Equation of Constant
Coefficient for Solving Some Model of Chemical Processes
Ali Yunus Rohedi 1
1
Department of Physics, Faculty of FSAD
Sepuluh Nopember Institute of Technology (ITS) Surabaya, Indonesia
1
Corresponding author: rohedi@physics.its.ac.id
Oral Presenter
ABSTRACT
Some chemical processes in batch reactor are commonly modelled in the form of Riccati differential
equation of constant coefficient. In order to provide analytical tool required for development the
model of chemical process, we need the exact formula of Riccati differential equation. In this paper
we introduce closed form formula for solution of Riccati differential equation of constant coefficient
as alternative method for analysing the chemical processes in batch reactor besides the common
procedure of series reaction sequence.
Keywords: reaction sequence, chemical processes, Riccati differential equation

83
Optimization of Zinc Oxide Nanoparicles Biosynthesis Using Saccharomyces
Cerevisiae with Box-Behnken Design
Fitry Mulyani, Safri Ishmayana, Diana Rakhmawaty Eddy
Department of Chemistry, Universitas Padjadjaran, Indonesia, 45363
Corresponding author: +62 813-2273-1173, diana.rahmawati@unpad.ac.id
Oral Presenter
ABSTRACT
Zinc oxide nanoparticles has wide application as catalyst, antimicrobial agent, drug delivery, etc. due
to its intrinsic properties. Various methods can be applied to synthesize nanoparticles, one of which
is biosynthesis process. Biosynthesis is one of alternative methods to prepare nanoparticle.
Biosynthesis is more eco-friendly over chemical and physical methods. In the present study
optimization of zinc oxide nanoparticles biosynthesis using the yeast Saccharomyces cerevisae was
performed by applying response surface method Box-behnken design (BBD). Three factors were
optimized in the present study including concentration of zinc acetate as precursor (X1), concentration
of S. cerevisiae fermentation broth (X2), and incubation time (X3). Mass of zinc oxide nanoparticles
(Y) was recorded as the response of the experiment. The product was then characterized by Fourier
Transform Infrared Spectroscopy (FTIR) and Particle Size Analyzer (PSA). The optimum condition
for the preparation of zinc oxide nanoparticles was found at 0.3 M, 100% (v/v) and 24 hours for zinc
acetate concentration, media concentration and incubation time, respectively. FTIR analysis showed
peaks at ~ 600 cm-1 which is characteristic for ZnO stretching. PSA analysis showed the smallest size
was 218.6 nm (12%) when the synthesis was performed at the optimum condition, while when the
incubation time was prolonged for 120 h, the size was decreased to 134.2 nm.
Keywords: biosynthesis, Box-Behnken, nanoparticles, Saccharomyces cerevisiae, ZnO

84
Compisition and Density of Marine Litter in Alor Island of Nusa Tenggara
Timur
Faryda Veronica Lamma Koly1, Alexandra Maheswari Waskita2, Paulus Edison Plaimo3, I Made
Dharma Jaya Aryawan
1
Department of Chemistry, Faculty of Math and Science, Universitas Tribuana Kalabahi, 85817
2
Marine and Fisheries Directorate, World Wildlife Fund, 12540
3
Department of Fishery, Faculty of Agriculture and Fisheries, Universitas Tribuana Kalabahi, 85817
Corresponding author: faryda.veronica.l.k@gmail.com/081238065856
Oral Presenter
ABSTRACT
The habit of trash littering as well as illegal waste dumping into the river and the ocean is commonly
found in various regions in Indonesia, including Alor island. River flow and water run-off causes
most of waste dumped on the land end up in the coastal and at the end is carried away to the ocean.
The inappropriate existence of anthropogenic waste causes damage to marine and coastal ecosystems,
for instance plastic ocean, while the ocean provides livelihood for most coastal people in Alor. In
addition, dirty coastal areas due to trash littering and illegal waste dumping can be another issue in
terms of marine tourism activity which is currently being promoted by the Alor Regency government.
Therefore, further research is needed in order to support the making of waste management regulation
as well as educational and awareness raising within people of Alor island. This research supported
by citizen science of Plastic Free Ocean Network Alor identified the composition and concentration
of marine litter in Alor island using the line transect method developed by CSIRO. The result showed
that the top three marine litter compositions in all locations during January-June 2019 were soft
plastic, hard plastic and glass. The density of marine litter on Alor Island is categorized as high
density as a whole. The location that contributed the highest marine litter density was Kadelang
Beach.
Keywords: Alor, citizen science, CSIRO, marine litter, plastic waste

85
Characteristics of Cepu Block Oil, Wonocolo Formation, East Java Indonesia :
Study of aliphatic biomarkers
Yulfi Zetra1,a , R.Y. Perry Burhan1,2, Awalia Wardatil Firdaus1,
Zjahra Vianita Nugrahaeni1
1
Molecular Geochemistry Laboratory, Department of Chemistry, Institut Teknologi Sepuluh Nopember,
Surabaya, Indonesia
2
PEM-AKAMIGAS CEPU, Indonesia
a)
Corresponding author: yzetra@gmail.com
Oral Presenter
ABSTRACT
The research of organic geochemistry has been carried out through the report of aliphatic
hydrocarbon fraction biomarkers to Cepu Block oil, Wonocolo Formation, East Java. Organic
geochemistry studies are carried out through biomarker analysis such as the depositional
environment, the origin of organic compounds, and the maturity of Cepu Block crude oil samples.
The biomarker was analyzed using the extraction, fractionation, and structure identification
methods using the Gas Chromatography-Mass Spectrometer (GC-MS) instrument. The odd-to-
even carbon number predominance of n-alkanes with a CPI value of 1.04 which indicates that the
organic material sample had low maturity. The indicator of maturity is also supported by the
Ts/Tm ratio of 0.52, and the abundance of 17α(H),21β(H)-hopane which is lower than
17β(H),21α(H)-hopane, and the high abundance of 5α(H)-7-cholestene. The pristane/phytane
(Pr/Ph) ratio of 3.25 and Ts/(Tm+Ts) ratio of 0.34 indicates that the oil sample came from an
oxidative depositional environment. The bimodal distribution of n-alkane biomarkers with a ratio
of long-chain to short chains n-alkanes (∑2/∑1) of 1.25 indicates that the source of oil organic
compounds comes from higher plants, bacteria, and algae. The presence of hopane and
homohopane, 8β(H)-drimane and homodrimane compounds, and also the sterane group indicates
the presence of bacterial input with bacteriohopanetetrol precursors produced by Archaebacteria.
The results that have been obtained indicate that the Cepu Block oil sample, Wonocolo Formation
has various organic sources with low maturity.
Keywords: Cepu Block oil, organic geochemistry, biomarkers, aliphatic hydrocarbons, KG-SM.

86
Synthesis and Characterization of Cr-Co/γ-Alumina Catalyst for Ethanol
Dehydration
Izza Aliyatul Muna1, Achmad Roesyadi1, a), M. Mahfud1, Firman Kurniawansyah1
1
Department of Chemical Engineering, Faculty of Industrial Technology and Systems Engineering, Sepuluh Nopember
Institute of Technology, Surabaya, East Java, 60111, Indonesia.
a)
Corresponding author: aroesyadi@yahoo.com
Oral Presenter
ABSTRACT
Primary alcohol dehydration reaction catalysis by using γ-Al2O3 catalyst can produce ether
compounds and alkenes. The combination chrome-cobalt and alumina catalyst could convert ethanol
to diethyl ether in the ethanol dehydration process. The purpose of this study was to determine the
performance of Cr-Co with the support of γ-Al2O3 catalyst for ethanol dehydration to diethyl ether.
Cr-Co/γ-Al2O3 catalyst was prepared using incipient wetness impregnation.γ-Al2O3 catalyst was
impregnated with chrome and cobalt metals at various metal loading 5 and 10% and impregnated
ratio of Cr-Co (1:1; 1:2; and 2:1). The samples were calcined at 550℃ for 3 hours in nitrogen and
then reduced continuously by hydrogen at 600℃ for 5 hours. Then catalyst was analyzed for
characterization using BET, XRD, and SEM-EDX. Treatment of γ-Al2O3 in various ratio of chrome
and cobalt impregnation decreased the total surface area. Increasing metal loading in Cr-Co
impregnation on γ-Al2O3 decreased total surface area and increased average pore diameter.
Impregnation process lowered the crystallinity but still maintained the material crystal structure. The
reduced surface area of the catalyst does not mean the effectiveness of the catalyst is reduced, but the
metal will actually increase the active site of the catalyst and will affect the rate of reaction in the
catalyst.
Keywords: ethanol, dehydration, γ-Al2O3, impregnation, catalyst

87
Synthesis, Characterization, and Identification of Metallic Soap (Zn-, Al-, and
Mg-Soap) and Its Potential as a Sunscreen
Sutrisno 1,a), Husni Wahyu Wijaya1,b), Dedek Sukarianingsih1,c), and Muhammad Nizar Dwi
Santiajo1,d)
1
Department of Chemistry Universitas Negeri Malang, Jl. Semarang 5 Malang 65145, Malang - Indonesia
a)
Corresponding author: sutrisno.kimia@um.ac.id
b)
husni.wahyu.fmipa@um.ac.id
c)
dedek.sukarianingsih.fmipa@um.ac.id
d)
rnizardwisantiaji@gmail.com
Oral Presenter
ABSTRACT
Zn-soap, Al-soap, and Mg-soap are known as synthetic metallic soaps. These substances cannot be
used as a surface-active agent or cleaning agent but has potential as the sunscreen active compound.
This research aims to synthesize metallic soap (zinc-, aluminum-, and magnesium-soaps) from
sunflower seed oil and test its activity as a sunscreen. The synthesized procedure was carried out with
the following steps (1) synthesis of K-soap through the saponification of oil with KOH, (2) synthesis
of metallic soap through the trans-saponification of soap-K with its chloride salts, (3) characterization
of physical properties and identification through IR and XRF spectra of synthesized metallic soap,
and (4) sunscreen activity test with UV spectrophotometry. The as-synthesized metallic soaps
resulted in the distinguished feature. The Zn-soap formed a white solid with melting point 72-76oC
and soluble in chloroform. Similar to Zn-soap, the Mg-soap melted at 102-120oC. However, the white
solid soap of Al-soap melted at 76-90oC and dissolved in chloroform and methanol. XRD patterns of
the as-synthesized Zn-soap showed the unique peaks from 2θ 4-12° that is unique for the layer Zinc
oleate, zinc linoleate, and zinc stearate structure. However, the broad peak from 2θ 14-28° was
dominant for Mg- and Al-soap. The metal content in the as-synthesized soaps that were analyzed
using the XRF revealed the percentage substitution of K-soap by Zn, Al, and Mg, i.e. 99, 76, and
81%, respectively. SPF value of Zn-soap, Al-soap, and Mg-soap sunflower seed oil was 2.78, 0.96,
and 1.63, respectively.
Keywords: metalic soap synthesis, Zn-soap, Al-soap, Mg-soap

88
“SIAB” Smart E-Stoichiometry As A Chemistry Learning Acid Base On
Android Mobile
Linaniyyatul Masruroh, Fikri Ramadhan,
Sagara Firdiantama, Berlian Al Kindhi
linaniyyatul.17104@mhs.its.ac.id
Oral Presenter
ABSTRACT
SIAB “Stoichiometry Acid Base” is a chemical calculator application that is useful for calculating
the quantitatively between reagents (reactants) and products (reaction products) in acid base reaction.
So far, the stoichiometry calculation is solved manually by referring to the periodic table. This
research, we want to make stoichiometric application that can be used for learning chemical reactions
easily, especially in acid base reaction. The application that we build can be used on Android
platform, so it can be accessed anytime and anywhere to facilitate learning. There are 3 step to
implementation, first step is problem survey, to find out whether the chosen problem is related to the
target. Second step is analysis, to make sure that the problem relates to the target, and the target is
experiencing the chosen problem. Last step is application design to build and design SIAB apps, for
the application design step there are 3 part of step. The first step, we search and gather sources that
contain about acid and base chemical reaction theory, which will be used as a reference material to
be displayed in the application. Then the second step is the fulfillment of the required application
assets including image and audio which for help user to make more interesting and understanding
easily. The third step is determining the core software used to make the application. From the results
of the implementation test of our application, 90% of students find this application helpful to
determine the results of chemical reactions between several molecules and easier to study.
Keywords: android, chemistry, digital, education.

89
Terpenoid from Indonesian Temu Mangga (Curcuma mangga, Val) Rhizomes
and Review of Its Anticancer Towards MCF-7 Breast Cells
Alicia Nathalia Angel Ganur1, Purwantiningsih Sugita1*, Dyah Utami Cahyaning Rahayu2, Hanhan
Dianhar3, and Ichsan Irwanto4
1
Department of Chemistry, Faculty of Mathematics and Natural Sciences (FMIPA), Institut Pertanian
Bogor, Dramaga Bogor 16680, Indonesia
2
3
Chemistry Study Program, Faculty of Mathematics and Natural Sciences (FMIPA), Universitas Negeri
Jakarta, Jakarta 13220, Indonesia
4
Natural Resources and Planning Unit, PT Hatfield Indonesia, Bogor, Indonesia
*
Corresponding authors’ email: purwantiningsih@apps.ipb.ac.id
Oral Presenter
ABSTRACT
Temu mangga (Curcuma manga Val) which belongs to Zingiberaceae family are largely distributed
in South East Asian countries including Indonesia. These plants have been traditionally used for
treatment of various diseases. In this research we focused to isolate terpenoid compounds from C.
mangga Val. rhizomes cultivated in Trop BRC garden LPPM-IPB, West Java, Indonesia in January
2019. C. mangga rhizomes were macerated in acetone then followed by increasing polarity partitions
with n-hexane, ethyl acetate, and methanol, respectively. n-Hexane fraction was further fractionated
by using various chromatography techniques to yield one isolated fraction. The isolated fraction then
was subjected by LCMS/MS. According to LC-MS/MS data, the isolated fraction was a mixture
consisted of terpenoids derivatives, i.e. zedoarondiol (1), curcumenol (2), curcumenone (3), and 13-
Hydroxygermacrone (4). Based on literature studies, terpenoids from Indonesian Curcuma Genus
could be developed as anticancer agent to inhibit MCF-7 breast cells’ growth.
Keywords: Curcuma mangga, terpenoid derivatives, anticancer, MCF-7 breast cells

90
Physicochemical Properties of Microcrystalline Cellulose Doped Imidazole
Membrane for Direct Methanol Fuel Cell
A. Priyangga1, A.B. Pambudi1, L. Atmaja1,a), J. Jaafar2
1
Departement of Chemistry, Faculty of Science and Data Analytics, Institut Teknologi Sepuluh Nopember,
Keputih Sukolilo, Surabaya, 60111, Indonesia
2
Advanced Membrane Technology (AMTEC) Research Centre, Universiti Teknologi Malaysia, 81310 UTM
Johor Bahru, Johor, Malaysia
a)
Corresponding author: lukman_at@chem.its.ac.id
Oral Presenter
ABSTRACT
Energy consumption is estimated to increase until 2040 mainly in fossil fuels usage as energy source.
Fossil fuels give negative impacts in human healthy and environment due to air pollution as the result
of combustion. Fuel cell is the one of many alternatives to produce cleaner energy. Nafion is the
membrane based perflourosulfonic acid which is still used as commercial electrolyte membrane for
fuel cell. There are several drawbacks that belongs to the nafion membrane such as high methanol
permeability and methanol uptake. The application of biomaterial microcrystalline cellulose (MC)
and the addition of imidazole (Im) are expected to diminish methanol permeability and increasing
the water uptake of the membrane. In this research, it is important to determine some composite
membrane’s performance which contains MC and Im. The composite membrane of MC/Im was
fabricated via phase inversion method using 5 wt.% H2SO4 coagulation bath then some membranes
was immersed in 2 wt.% phosphotungstic acid (PTA) solution. The lowest methanol permeability
and methanol uptake is obtained by MC/Im membrane with 4,67×10 -7 cm2.s-1 and 3.19%,
respectively. The water uptake of MC/Im achieves 45.52% which is higher than MC and MC/PTA
at 35.94% and 41.75%, respectively. Based on the MC/Im properties, the membrane has high
potential to be a new composite membrane for fuel cell.
Keywords: Cellulose, imidazole, composite membrane, fuel cell

91
Alkaloid and Terpenoids From Indonesian White Turmeric (Curcuma zedoaria)
Rhizomes and Its Potency as Antibacterial Agent
O. D. Syurga1, D. A. Setyani2, G. Syahbirin1, D. U. C. Rahayu2,
H. Dianhar3 and P. Sugita1*
1
Department of Chemistry, Faculty of Mathematics and Natural Sciences (FMIPA), IPB University, Bogor,
Indonesia
2
Depok, Indonesia
3
Chemistry Study Program, Faculty of Mathematics and Natural Sciences (FMIPA), Universitas Negeri
Jakarta, Jakarta, Indonesia
*
Corresponding authors’ email: purwantiningsih@apps.ipb.ac.id
Oral Presenter
ABSTRACT
Curcuma zedoaria (Zingiberaceae), also known as white turmeric, has been cultivated as a spice and
vegetable in South and Southeast Asia including Indonesia. It is commonly used traditionally for
treatment of various diseases. The objective research to isolate alkaloid and terpenoids from C.
zedoaria rhizomes cultivated in Trop BRC garden LPPM-IPB, West Java, Indonesia and reviewed
by literature studies its potency as antibacterial agent. C. zedoaria rhizomes were macerated in
methanol then followed by increasing polarity partitions with n-hexane, ethyl acetate, and methanol,
respectively. n-Hexane fraction was further fractionated by using various chromatography techniques
to yield one isolated fraction. The isolated fraction then was subjected by LCMS/MS. According to
LC-MS/MS data, the isolated fraction was afforded as a mixture consisted of alkaloid and terpenoids
derivatives, i.e. piperine, curzerenone and curcumenol. The literature studies revealed that C.
zedoaria rhizomes extract has a higher potential as an antibacterial against gram-positive than gram-
negative bacteria. In conclusion, Indonesian C. zedoaria contained alkaloid and terpenoids which
could be developed as antibacterial agent according to literature review.
Keywords: Curcuma zedoaria, piperine, curzerenone, curcumenol, antibacterial

92
Physicochemical Properties of Prehistoric Rock Art Pigments in Jupri Cave,
Sangkulirang, Indonesia
Evi Maryantia,1, Moh Mualliful Ilmia, Nadya Nurdinia, Pindi Setiawanb, Chatree Saiyasombatc,
Grandprix T.M. Kadjaa,d, Yana M. Syahe and Ismunandara
a
Division of Inorganic and Physical Chemistry, Institut Teknologi Bandung, Jl. Ganesha 10, Bandung
40132, Indonesia
b
Division of Visual Communication and Multimedia, Institut Teknologi Bandung, Jl. Ganesha 10, Bandung
40132, Indonesia
c
Synchrotron Light Research Institute, 111 University Ave, Muang, Nakhon Ratchasima 30000, Thailand
d
Research Center for Nanosciences and Nanotechnology, Institut Teknologi Bandung, Jl. Ganesha 10,
Bandung 40132, Indonesia
e
Division of Organic Chemistry, Institut Teknologi Bandung, Jl. Ganesha 10, Bandung 40132, Indonesia
*Coresponding Author: kadja@chem.itb.ac.id,ismu@chem.itb.ac.id
Oral Presenter
ABSTRACT
The Sangkulirang site, East Kalimantan, has hundreds of rock art images with various motifs and
hues which are spread in more than fifty caves, including the Jupri caves. In this study, various
analytical characterization were utilized to determine the physicochemical properties of the rock art
pigments with different colors, i.e., red, purple, and black hues in Jupri Cave. The analytical
characterizations, including Synchrotron Radiation X-ray Diffraction (SR-XRD), XRF, FTIR, SEM
and XANES, showed that the pigments from the Jupri caves contain hematite (Fe2O3) as the main
component of the pigment, with gypsum (CaSO4.2H2O) and calcite (CaCO3) as the rock base. The
results indicated the differences in content, concentration, crystallinity, and hematite particle size in
the three pigment samples. These different physicochemical properties contribute to pigment hue
variations. More detailed chemical studies are needed to explain the source of the pigment starting
material and preparation techniques in the manufacture of pigments.
Keywords: Rock art, physicochemical properties, Jupri cave, hematite, pigment.

93
Detailed studies of prehistoric rock art pigments in Tewet Cave, Sangkulirang
site, East Kalimantan, Indonesia
Nadya Nurdinia, Evi Maryantia,1 , Moh. Mualliful Ilmia, Pindi Setiawanc, Chatree Saiyasombatd,
Grandprix T.M. Kadjaa,e, Ismunandara
a
Division of Inorganic and Physical Chemistry, Institut Teknologi Bandung, Jl. Ganesha No. 10, Bandung
40132, Indonesia.
c
Division of Visual Communication and Multimedia, Institut Teknologi Bandung, Jl. Ganesha No. 10,
Bandung 40132, Indonesia.
d
Synchrotron Light Research Institute, 111 University Ave, Muang, Nakhon Ratchasima, Thailand
e
Research Center for Nanosciences and Nanotechnology, Institut Teknologi Bandung, Jl. Ganesha No. 10,
Bandung 40132, Indonesia.
f
Center for Catalysis and Reaction Engineering, Institut Teknologi Bandung, Jl. Ganesha No. 10, Bandung
40132, Indonesia
*corresponding author: kadja@chem.itb.ac.id, ismu@chem.itb.ac.id
Oral Presenter
ABSTRACT
Recent reports have suggested that the oldest figurative prehistoric rock art is located in Indonesia.
Tewet cave, located in Sangkulirang site-Kalimantan, is one of the notable rock art sites in Indonesia.
Three colors (red, brick red, and purple) from Tewet cave have been investigated using several
advanced instruments (Synchrotron XRD, Fe K-edge XANES, XRF, SEM, and FTIR) to identify the
physicochemical characteristics. The Synchrotron-XRD was used to identify the minerals whereas
Fe K-edge XANES was carried out to obtain the local geometries and electronic structure
information. Further physicochemical characterizations were performed on XRF (the chemical
compositions), FTIR (the bonding and functional groups), and SEM (morphology). Based on the
overall results of the characterization, hematite was the pigment's main component while gypsum
and calcite composed the rock substrate. The color difference of hematite was generated by the
distortion of the octahedral geometry. This distortion occurs due to an energy shift between the t 2g
and eg orbitals which produced different hues. The purple sample has the highest geometry distortion
among other samples followed by brick red and red samples, respectively. This phenomenon is likely
to be induced by different thermal treatment of red pigment, either deliberately or unintentionally,
resulted in purple pigment. We anticipated this study to provide more comprehensive information in
understanding the chemistry and engineering of prehistoric rock art pigments.
Keywords: hematite, physicochemical characteristics, pigment, rock art, synchrotron.

94
Green Coke Graphite as Anode in Lithium Ion Battery
Fajar Rifqi Fadhilaa, Rahmat Wibowoa, Hanida Nilasaryb, Achmad Subhanc, Yuni Krisyuningsih
Krisnandia
F R Fadhila1, R Wibowo1, H Nilasary2, A Subhan3, Y K Krisnandi1
1
SIF Laboratory Department of Chemistry FMIPA Universitas Indonesia, Depok 16424, Indonesia
2
Research & Technology Center - PT. Pertamina (Persero), Indonesia
3
Pusat Penelitian Fisika LIPI, Kawasan Puspitek, Serpong, Tangerang Selatan 15314, Indonesia
yuni.krisnandi@sci.ui.ac.id
Oral Presenter
ABSTRACT
Lithium-ion battery as an energy storage platform has been developed in the last 2 decades with
variations in electrodes composition. Lithium-ion battery as an energy storage platform has been
developed in the last 2 decades with variations in electrodes composition. This battery could be
optimized up to 80% of its ability in storing energy. Anode material that commonly used in lithium
ion battery is graphite, having a layered structure that can maximize the intercalation process of
lithium ions. Graphite has been successfully synthesized from green coke which is a by-product of
thermal cracking process used by petroleum companies to change fuel oil residues. Green coke
graphite synthesis was carried out by mixing green coke material with Super P as conductive carbon,
Polyivinylidine Fluoride (PVDF) as binder (8:1:1), and N-N Dimetyl Acetamid (DMAC) as solvent,
then used as anode sheet on coating stage with copper foil using doctor blade. Commercial graphite
were also used as anode sheet as comparison. The green coke anode was characterized using FTIR,
XRD and SEM-EDS. Electrochemical performance was characterized using CV, GCD, and EIS.
Cycling performance of green coke anode in Li-ion batteries produces reversible capacity and
coulombic efficiency of 202.59 mAh g-1 and 79.77 %, respectively. Green coke anode produce lower
coulombic efficiency when compared to graphite anode (91.51%). However, the combination of the
use of petroleum waste as raw material and good electrochemical performance would make graphite
green coke a promising material for a low cost battery for large scale energy storage.
Keywords: Graphite, Green Coke, Electrochemical, Anode, Lithium-ion battery

95
Intramolecular Hydrogen bond and Vibrational Spectroscopic Study of
Cellulose Oligosaccharide Using Density Functional Theory
Agung Bagus Pambudi1, Arif Priyangga1, Djoko Hartanto1 and Lukman Atmaja1, *)
1)
Department of Cemistry, Institut Teknologi Sepuluh Nopember, Surabaya, Indonesia
*)
E-mail: lukman.at@gmail.com
Oral Presenter
ABSTRACT
The structural geometry, intramolecular hydrogen bond (H-bond) and IR spectra of cellulose
oligosaccharide with degree of polymerization up to 5 are studied by density-functional theory (DFT)
at CAM-B3LYP/6-31G(d) level in this study. Bader's QTAIM is used to elucidate long-range forces
of non-bonding atom such as intramolecular H-Bond and van der Waal's interaction. The electrostatic
potential map is used to depict the reactive sites of the oligosaccharides. A comparative experimental
and theoretical approach was used validate the IR spectra of the cellulose oligomer. Based on
calculated Infrared spectra, the predicted spectra have good agreement with the available
experimental data. This research has significant importance on characterization of oligomeric
cellulose for specific purposes such as mechanistic study of proton transport and ion adsorption that
close related to intramolecular H-Bond.
Keywords: Cellulose, DFT, Hydogen Bond, IR Spectra, Oligosacharide

96
Reactive Oxygen Species (ROS) Hydrogen Peroxide Scavenger Activities of Dimeric Complex
(µ-4,4'-Bipyridine)-Bis{Perchlorate-Salen-Manganese(III)} Through Vitamin C Inhibition
Alya Rafida, Yusi Deawati, Diana Rakhmawaty Eddy
Padjadjaran University , Indonesia
rafidaalya@gmail.com
Oral Presenter
ABSTRACT
Manganese(III)-salen complex compounds and their derivatives have been extensively studied and
have shown quite a high ability in counteracting reactive oxygen species (ROS), such as hydrogen
peroxide (H2O2). However, research on the ability of manganese (III)-salen complex compounds as
a scavenger to H2O2 is still limited to compounds that have a monomeric structure. Meanwhile, one
dimeric complex, containing 4,4'-bipyridine ligand bridge, has been found its crystal structure, which
is complex (µ-4,4'-bipyridine)-bis{perchlorate-salen-manganese(III)},[{Mn(salen)(ClO4)}2(µ-4,4'-
bpy)]. The complex has only been tested for one type of ROS, superoxide radicals, and showed very
high activity. Thus, it is very interesting to know the dimeric complex activity against ROS H2O2.
Therefore, in this study, the ability of dimeric complex compounds [{Mn(salen)(ClO 4)}2(µ-4,4'-
bpy)], was being tested to scavenge H2O2. The dimeric complex [{Mn(salen)(ClO4)}2(µ-4,4'-bpy)]
was synthesized from the monomeric complex [Mn(salen)(H2O)Cl].H2O reacted with 4,4'-bipyridine
in the methanol solvent and saturated potassium perchlorate. From this study,
[{Mn(salen)(ClO4)}2(µ-4,4'-bpy)] black-brown solid was obtained with the yield of 39.04%, and
have composition of elements C = 50.47% (50.57%); H = 3.35% (3.64%); and N = 8.47% (8.42%),
which according to the theoretical percent in parentheses. While the IR spectrum of the compound
of the synthesized complex showed the presence of Mn-N, Mn-O dan ClO4 bonds, respectively at
wave numbers of 471.9 cm-1; 597.9 cm-1 and 1032.4-1147.2 cm-1. At the last step, the H2O2 scavenger
activity showed that the dimeric complex of [{Mn(salen)(ClO4)}2(µ-4,4'-bpy)] can scavenge the
reaction of vitamin C with ROS H2O2 or have %inhibition of 24%.
Keywords: scavenger, H2O2, manganese(III)-salen, 4,4'-bipyridine, perchlorate, dimeric

97
Kinetics of Citronella Oil Extraction from Cymbopogon nardus Leaves using
Microwave-Assisted Green Technique
Haqqyana Haqqyanab), Anisa Estu Murbawani, Hasna Nabilla, Ali Altway, Mahfud Mahfuda)
Institut Teknologi Sepuluh Nopember, Indonesia.
a)
mahfud@chem-eng.its.ac.id
b)
haqqyana.19022@mhs.its.ac.id
Oral Presenter
ABSTRACT
As a native plant to the subtropical and tropical areas, Cymbopogon nardus is widely cultivated
and utilized for pharmaceutical purposes. Microwave-assisted hydrodistillation (MHD) was
preferred as a greener method to extract essential oils, compared to its conventional
alternatives. The yields and kinetics of essential oil extraction from Cymbopogon nardus leaves
were studied under different values of microwave power at atmospheric pressure. Forty grams
of feed was introduced to a distilled flask containing a 500mL of water solvent upon extraction.
Each experiment was conducted for a total of 180 min. irradiation time with 30 min. intervals.
This method facilitated a high yield of citronella oil (4.12-4.24%) with a shorter extraction
time. Known kinetic models such as pseudo-first and pseudo-second-order kinetic models were
used to model the citronella oil extraction process. Results indicated that the studied models
gave a relatively good fit to the experimental data, with the pseudo-second-order kinetic model
showed a general better fit. Thus, the model could be used to describe the extraction kinetics
of citronella oil from Cymbopogon nardus leaves. Moreover, GC-MS analysis of the citronella
oil showed that the main constituents obtained were geraniol, isohomogenol, citronella, and b-
citronellol. These results suggested that the MHD method could be a prominent alternative to
provide essential oils in higher quality and quantity at reduced time.
Keywords: Citronella oil, Microwave-assisted hydrodistillation, kinetic modelling,

Cymbopogon nardus leaves, green extraction

98
Isolation of Cellulose from Teak Wood Using Hydrothermal Method
Prida Novarita Trisanti(a), Muhammad Rifan, Pandu Akbar, Ignatius Gunardi and Sumarno
Department of Chemical Engineering, Institut Teknologi Sepuluh Nopember, Surabaya, East Java, 60111,
Indonesia
Corresponding author: pridanova@chem-eng.its.ac.id
Oral Presenter
ABSTRACT
Teak wood is a type of hard wood that is abundant in Indonesia. Generally, this wood is widely
used for housing structures and furniture. But from these uses, the resulting waste has not been
widely used. One of the uses of this waste is to isolated of the cellulose contained in teak wood,
thus giving it a high value later. Teak wood powder was isolated through a base delignification
process followed by hydrothermal to obtain good quality cellulose. This study aims to determine
the characteristics of cellulose resulting from the combination of these processes. In the base
delignification process, wood powder is processed using 0.2 M NaOH at 40OC for 30 minutes. In
order for the process to run optimally, this process is assisted by ultrasound. Then proceed with
the hydrothermal process at a pressure of 100 bar and a temperature of 200OC for various times.
From the Chesson analysis it can be seen that the composition of lignin and hemicellulose was
decreased. And from the scanning electron microscopy analysis, it can be seen that the resulting
cellulose is in the form of rods with the highest crystallinity degree of 79.33%.
Keywords: Teak wood, cellulose, isolation, base-delignification, hydrothermal process

99
Synthesis of NiCl2 Impregnated Mesoporous Carbon and Its Adsorption Activity
on CO2
A Afriani1, I Abdullah1,a), Y K Krisnandi1
1
Department of Chemistry, FMIPA Universitas Indonesia, Kampus UI Depok, Depok 16424, Indonesia
a)
Corresponding author: iman.abdullah@sci.ui.ac.id
Oral Presenter
ABSTRACT
The continuous increase of CO2 concentrations in the atmosphere for decades has influenced the
global climate change.Efforts to overcome the high concentration of CO2 need to be done, one
of which is the Carbon Capture and Storage (CCS) method through CO2 capture on an
adsorbent. In this research, mesoporous carbon impregnated with NiCl2 (NiCl2/MC) was employed
for the adsorption of CO2 gas. Mesoporous carbon was successfully synthesized using soft template
method which was evidenced by the results of the XRD analysis which showed typical diffraction
patterns of carbon materials which were 25.68o and 43.26o with the Miller index of (002) and (100),
respectively. Mesoporous carbon materials and NiCl 2/MC were characterized by FT-IR, XRD, SAA
and SEM-EDX. The results of the SAA characterization showed that NiCl2/MCbelongs to materials
that have meso-sized pores with the pore size of 9.6 nm. The CO2 adsorption test using MC and
NiCl2/MC were carried out by passing CO2 gas through the adsorbent for 15 minutes and the result
showed that MC material obtained a value of 0.46 mmol CO2 per gram of adsorbent while NiCl2/MC
was able to obtain a value of 8.90 mmol CO2 per gram of adsorbent.
Keywords: Mesoporous Carbon, NiCl2, impregnation, CO2 capture, adsorption

100
The Effect of Alkaline Concentration in the Alkaline Delignification-Assisted
Sonication on Sengon Wood
Prida Novarita Trisanti(a), Muhammad Rifan, Pandu Akbar, Ignatius Gunardi and Sumarno(b)
Department of Chemical Engineering, Institut Teknologi Sepuluh Nopember, Surabaya, East Java, 60111,
Indonesia
(a)
Corresponding author: pridanova@chem-eng.its.ac.id
(b)
onramus@chem-eng.its.ac.id
Oral Presenter
ABSTRACT
Sengon wood is classified as soft wood because of its characteristics and is widely
used in Indonesia. Utilization of Sengon wood produces wood dust that can still be
used because of its high cellulose content. Sengon wood contains 41.17% cellulose,
22.26% hemicellulose and 17.51% lignin. In this study, cellulose extraction was
carried out using alkaline delignification-assisted ultrasound methods. The effect of
alkaline concentration was studied to obtain cellulose with good characteristics. The
delignification process was carried out by mixing sawdust with NaOH solution
(1:30 w/v). The process was carried out at 40 oC for 30 minutes with various
concentrations of NaOH (0.2 M - 0.7 M). Process results were analyzed using
Chesson analysis, X-ray Diffraction (XRD), Fourier-transform infrared
spectroscopy (FTIR) and Scanning electron microscopy (SEM). Based on the data,
the highest decreasing of hemicellulose and lignin composition was achieved at the
lowest concentration of NaOH (0,2 M). Morphologically, it appears that alkaline-
delignification-assisted ultrasound is able to expand contact area of NaOH solution
and tear the surface structure.
Keywords: Sengon wood, Alkaline, Delignification, Ultrasound, Cellulose

101
Synthesis of Bimetallic Ni-Co Modified Mesoporous Carbon and Its Catalytic
Activity for Selective Hydrogenation of Phenylacetylene to Styrene
Iman Abdullah*, T M Ratu, M Ridwan, Y K Krisnandi
Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Indonesia, Indonesia
iman.abdullah@sci.ui.ac.id
Oral Presenter
ABSTRACT
Polystyrene, a polymer composed of styrene monomer, is widely used as the main raw material in
food container production. However trace quantities of phenylacetylene in styrene can poison the
catalyst used in the styrene polymerization process. Therefore, it is necessary remove it, for instant
by catalytically converting it to styrene using selective hydrogenation method. Mesoporous carbon
(MC) has been successfully synthesized via soft template method using phloroglucinol and
formaldehyde as carbon precursors, and pluronic F127 as mesopore template. The as-synthesized
MC then impregnated with bimetallic Ni-Co(NiCo/MC), followed by reduction for 4 hour at 400 °C
under a flow of H2. The catalysts were characterized using FTIR, XRD, SEM-EDX, SAA and TEM.
Both MC and bimetallic NiCo/MC then were used as catalysts in phenylacetylene selective
hydrogenation at 303 K and 323 K for 5 h with H2 as the reducing agent. The as-synthesized MC
modified monometallic Ni (Ni/MC) was also tested as comparison. The reaction product was
measured using gas chromatography with flame ionization detector. The results show that the
NiCo/MC catalyst gives a yield of 1.42% and 1.33% for the reaction at 303 and 323 K, respectively.
Whereas Ni/MC catalyst gives a higher yield of 4% for the reaction at 323 K.
Keywords: Mesoporous Carbon, Hydrogenation Reaction, Bimetallic NiCo, Phenylacetylene,

Styrene

102
Optimization of Non-Catalytic In-Situ Biodiesel Production from Rice Bran
Using Historical Data Design (HDD)
Ari K. Putra, Fathi H. Fachrudin, Siti Zullaikah*, Rosada Y. Naulina, Sri Utami, Rifky P.
Herminanto, Orchidea Rachmaniah
Department of Chemical Engineering, Institut Teknologi Sepuluh November (ITS), Kampus ITS Keputih
szulle@chem-eng.its.ac.id, szullehakim@gmail.com
Oral Presenter
ABSTRACT
Biodiesel has become one of the essential fuels in the present and future scenario and it can be
produced from vegetable oil and animal fat. However, current feedstocks to produce biodiesel slow
the growth of biodiesel blend implementation due to the high cost of feedstock production. As a
result, rice bran oil is claimed to be a potential feedstock for high yield biodiesel. In this study, a
mixture of subcritical water-ethanol mixture with co solvent and CO2 as pressurizing gas was
employed in converting rice bran to biodiesel. The full process of biodiesel production was carried
out by response surface methodology (RSM) in conjunction with the historical data design (HDD)
for statistically optimization and modeling. From the ANOVA, it was found the yield of biodiesel
achieved an optimum level of 95.26% at 160oC, under a specific optimized condition of ethanol-
water concentration 79.59% (v/v) and ethyl acetate as co solvent. By increasing temperature and time
of reaction, it was found that the yield of biodiesel achieved an optimum level of 113.08% using ethyl
acetate as co solvent and ethanol-water concentration of 80% (v/v), under a specific optimized
condition of 2.50 h and 200oC.
Keywords: Rice bran; Biodiesel; Historical data design; Subcritical ethanol-water mixture

103
Analysis Of Cd Metal Ions Using Screen Printed Electrode (SPE) Modified With
4-Carboxyphenyl Diazonium Salt
Dessy Armadanie, Rahmat Wibowo, Dita Arifa Nurani(a)
Department Chemistry, Faculty Mathematics and Natural Svience, University of Indonesia, UI Campus
Depok 16424 Indonesia
a)
Oral Presenter
ABSTRACT
Screen Printed Electrode (SPE) has several advantages in chemical sensing application such as
has a small size, low cost, and can be used for in-situ analysis. In this research, SPE was modified
with 4-Carboxyphenyl Diazonium Salt by cyclic voltammetry at potential +1,5 V to -1,0 V for 3
cycle (SPE/4-CPD CV). The other electrodeposition method of 4-CPD was conducted by
chronoamperometric at potential -0,6 V for 300 s (SPE/4-CPD Ch). Diazonium salt was chosen
for modification on the SPE surface because of its stability to be modified on both conductive and
semi conductive surfaces. The modified electrodes was characterized by FTIR and SEM-EDX
prior to its application in Cd (II) analysis. Cd (II) detection was achieved by immersing the
deposited electrode into the sample solution, followed by the electrochemical measurement of
adsorbed Cd (II) by cyclic voltammetry. The described method provided the linearity of Cd (II)
analysis 0,9984 for SPE/4-CPD CV and 0,9935 for SPE/4-CPD Ch in concentration range of
0,001-0,005 ppm. The sensitivity of SPE/4-CPD CV 0,0049 was higher than SPE/4-CPD Ch
0,004. The performances of modified electrodes indicates that SPE/4-CPD CV and SPE/4-CPD
Ch could be considered as a tool in metal ions analysis.
Keywords: Screen Printed Electrode, Heavy Metals, 4-Carboxyphenyl Diazonium Salt,

Electrodeposition, Cyclic Voltammetry.

104
IIn Vitro Free Radical Scavenging Activity of Flavonoid Glycoside Compounds
from The Leaf Extract of Protium Javanicum Burm. F
N.M. Puspawati, I.A. Raka Astiti Asih, I.D.G.Yoga Paramatha
Universitas Udayana, Indonesia
made_puspawati@unud.ac.id
Oral Presenter
ABSTRACT
Protium javanicum Burm. F, locally known as tenggulun, is one of the Indonesian medicinal plants
that contains flavonoids as a source of natural antioxidant. This research aims to determine in vitro
free radical scavenging activity (IC50) of n-hexane, ethyl acetate, and n-butanol extracts of tenggulun
leaf and identify the flavonoid compounds present in the most active extract. In vitro free radical
scavenging activity (IC50) was done using DPPH (2,2-diphenyl-1-pycrylhidrazyl), and identification
of the flavonoid compounds using LCMS/MS (Liquid Chromatography-Mass Spectrometry/Mass
Spectrometry). The results demonstrated that the ethyl acetate extract gave the highest free radical
scavenging activity with (IC50) value of 11.86 ppm followed by n-butanol 19.73 ppm, and n-hexane
25.52. Separation of the ethyl acetate active extract was initially done using vacuum liquid
chromatography with gradient elution from 100% of n-hexane to 100% of methanol producing 6
combined fractions (A, B, C, D, E, and F). The fraction E showing the most flavonoid content was
further purified by column chromatography silica gel with ethyl acetic: acetic acid: formic acid: water
(100: 10: 10: 26) as a mobile phase and 10 fractions were obtained. Fractions EC, EG, and EI which
showed relatively pure based on thin layer chromatography analysis were then identified. Based on
the mass spectra data, the fractions of EC, EG, and EI were tentatively identified as flavonoid glycoside
quercitrin, kaemferol-3-O-rutinoside, and rutine respectively. These compounds may act
synergistically to scavenge free radicals.
Keywords: Glycosides Flavonoids, Protium javanicum Burm.F, LC-MS/MS

105
Computational Investigation of Interaction between β-Cyclodextrin and
Hydroxychloroquine
Atthar Luqman Ivansyah1,2
1
Master Program in Computational Science, Faculty of Mathematics and Natural Sciences, Institut
Teknologi Bandung, Jalan Ganesha No. 10, Bandung, West Java, 40132
2
Analytical Chemistry Research Group, Department of Chemistry, Faculty of Mathematics and Natural
Sciences, Institut Teknologi Bandung, Jalan Ganesha No. 10, Bandung, West Java, 40132
atthar@compscience.itb.ac.id
Oral Presenter
ABSTRACT
At the end of 2019, there was an outbreak with symptoms similar to SARS in the People's Republic
of China caused by the SARS-CoV-2 virus. The massive spread of the virus, including in Indonesia,
caused WHO to issue a pandemic status. One alternative to reducing COVID-19 patients is to use
existing drugs, until a vaccine is found and ready for use. Currently, hydroxychloroquine has been
widely researched as a candidate for anti-COVID-19 drugs, which has now entered the clinical trial
stage. However, the solubility of hydroxychloroquine in water is so small that it has an impact on its
low bioavailability in the human body. One way to increase the solubility of hydroxychloroquine in
water is through the formation of stable inclusions complex with β-cyclodextrin. In this research, a
molecular docking study was conducted to investigate the interaction between β-cyclodextrin and
hydroxychloroquine. The results of the molecular docking study show that β-cyclodextrin and
hydroxychloroquine form stable inclusion complex, with the binding energy value of 4.792 kcal/mol
and dissociation constant value of 0.307 mM.
Keywords: SARS-CoV-2, COVID-19, Hydroxychloroquine, β-Cyclodextrin, Inclusion Complex

106
Theoretical Investigation of Interaction between Hydroxypropyl-β-Cyclodextrin
and Hydroxychloroquine
Atthar Luqman Ivansyah1,2
1
Master Program in Computational Science, Faculty of Mathematics and Natural Sciences, Institut
Teknologi Bandung, Jalan Ganesha No. 10, Bandung, West Java, 40132
2
Analytical Chemistry Research Group, Department of Chemistry, Faculty of Mathematics and Natural
Sciences, Institut Teknologi Bandung, Jalan Ganesha No. 10, Bandung, West Java, 40132
atthar@compscience.itb.ac.id
Oral Presenter
ABSTRACT
At the end of 2019, there was an outbreak with symptoms similar to SARS in the People's Republic
of China caused by the SARS-CoV-2 virus. The massive spread of the virus, including in Indonesia,
caused WHO to issue a pandemic status. One alternative to reducing COVID-19 patients is to use
existing drugs, until a vaccine is found and ready for use. Currently, hydroxychloroquine has been
widely researched as a candidate for anti-COVID-19 drugs, which has now entered the clinical trial
stage. However, the solubility of hydroxychloroquine in water is so small that it has an impact on its
low bioavailability in the human body. One way to increase the solubility of hydroxychloroquine in
water is through the formation of stable inclusion complex with hydroxypropyl-β-cyclodextrin. In
this research, a molecular docking study was conducted to investigate the interaction between
hydroxypropyl-β-cyclodextrin and hydroxychloroquine. The results of the molecular docking study
show that hydroxypropyl-β-cyclodextrin and hydroxychloroquine form stable inclusion complex,
with the binding energy value of 4.808 kcal/mol and dissociation constant value of 0.299 mM.
Keywords: SARS-CoV-2, COVID-19, Hydroxychloroquine, Hydroxypropyl-β-Cyclodextrin,

Inclusion Complex

107
Antibacterial Activity of Flavonoids from Ethyl Acetate Extract of Milk Banana
Peel(Musa x paradisiaca L.)
1*
Wiwik Susanah Rita, 1Ida Ayu Raka Astiti Asih, 2I Made Dira Swantara, 1Ni Luh Yuli Damayanti
1
Chemistry Department, Faculty of Mathematic and Natural Sciences Universitas Udayana Badung, Bali,
Indonesia
2
Magister of Chemistry Department, Faculty of Mathematic and Natural Sciences, Universitas Udayana
Badung, Bali, Indonesia
*Corresponding author: susanah.rita@unud.ac.id
Oral Presenter
ABSTRACT
Infectious diseases caused by Staphylococcus aureus and Escherichia coli can be treated by milk
banana peels ((Musa × paradisiaca L.). This study aims to reveal the antibacterial activity of milk
banana peel ethyl acetate extract against Staphylococcus aureus and Escherichia coli, and to identify
their active compounds. Extraction was conducted by maceration at room temperature, followed by
partition. Separation was carried out by column chromatography with mobile phase of chloroform:
ethyl acetate (7.5:2.5) and stationary phase of silica gel 60. Antibacterial assay was performed by
well diffusion method and identification of active compounds was analyzed by UV-Vis
spectrophotometry and LC-MS/MS. One kg of dried powder of milk banana peels was extracted with
8 L methanol produced 124.70 g of crude extract. The extract was dissolved into methanol 70%, then
the methanol was evaporated so that water extract remained. The water extract was partitioned with,
n-hexane, ethyl acetate, and n-butanol respectively. Antibacterial activity assay to the fractions shows
that ethyl acetate extract was the most active to inhibit the growth of both bacteria. The MIC of the
extract was 1% with inhibition of 5.00 mm. Separation with column chromatography resulted 5
fractions. Identification of the most active fraction with UV-Vis showed that the isolate gave
maximum absorption at λ 339.00 nm (band I) and λ 262.00 nm (band II) which were thought to be
flavonol (3-OH substituted). Analysis with LC-MS/MS shows that the most active fraction contained
3-methacrylate flavonols and 3-(furan-2yl) acrylate flavonols.
Keyword: Flavonoids, Antibacterial Cctivity, Musa × paradisiaca L., Staphylococcus aureus,

Escherichia coli

108
Investigation of Envelope Protein Gene in Indonesian Severe Acute Respiratory
Syndrome Coronavirus 2 Isolates: A Bioinformatics Study
Arif Nur Muhammad Ansori
Faculty of Veterinary Medicine, Universitas Airlangga, Indonesia
arif.nma-17@fkh.unair.ac.id / +6282144647832
Oral Presenter
ABSTRACT
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the seventh coronavirus that has
crossed the species barrier to infect human populations. It is a rapidly spreading virus that has poses
a significant public threat and is a considerable burden on the global economy and human health. We
characterized the envelope (E) protein gene of Indonesian SARS-CoV-2 isolates to investigate the
genetic composition and construct a molecular phylogenetic analysis. In the present work, we
retrieved the Indonesian SARS-CoV-2 isolates from the GISAID EpiCoVTM and the Wuhan-Hu-1
isolate (reference sequence) from GenBank®, NCBI. We analyzed nucleotide variants and amino acid
changes using Molecular Evolutionary Genetics Analysis (MEGA) X and analyzed gene similarity
using the LALIGN web server. Then, we used MEGA X to construct a molecular phylogenetic
analysis. Interestingly, we report the genetic composition of the E protein gene in Indonesian SARS-
CoV-2 isolates. Furthermore, we also presented the molecular phylogenetic tree of Indonesian
SARS-CoV-2 isolates and other coronaviruses. In summary, we propose that there were no
significant changes in the genetic composition of the SARS-CoV-2 E protein gene when compared
to the Wuhan-Hu-1 isolate from China. However, this is a preliminary study and we recommend that
molecular epidemiology and surveillance programs against COVID-19 in Indonesia be improved.
Keywords: COVID-19, envelope protein gene, SARS-CoV-2

109
A comparison of nine different models to correlate the solubility of azobenzene
compounds in supercritical carbon dioxide
Ratna Surya Alwi and Andi Sry Iryani
ratnasya@gmail.com
Oral Presenter
ABSTRACT
Supercritical technologies have been developed in the textile industry during recent decades. To
develop supercritical technology, solubilities of dye compounds in supercritical carbon dioxide and
their correlations are highly essential. The present study aims are to compare nine different empirical
and semiempirical models (Chrastil, Kumar and Johnston, Adachi and Lu, del Valle and Aguilera,
Sung and Shim, Sparks et al., Garlapati and Madras, Bian et al., and Si-Moussa et al.) as a function
of density and temperature to correlate the solubility of azo dyes in supercritical carbon dioxide.
Results indicated that the model developed by Adachi and Lu is the best in terms of correlating the
experimental solubility of the Azo compounds in Supercritical carbon dioxide.
Keywords: Supercritical carbon dioxide, Azo compounds, empirical and semi-empirical models.

110
Oleic-Imidazoline Synthesis and Its Performance as Corrosion Inhibitor
towards Carbon Steel
S Fitriani, S Cahyani, D U C Rahayu*, I Abdullah, D A Nurani, and Y K Krisnandi
16424, Indonesia
Oral Presenter
ABSTRACT
Carbon steel is the principal material used in oil and gas pipelines for production lines. However,
carbon steel is vulnerable to corrosion due to its corrosive media which is causing economic losses.
Corrosion rate can be slow down using imidazoline, one of the organic corrosion inhibitors. In this
study, oleic-imidazoline was successfully synthesized from triethylenetetramine (TETA) and oleic
acid (OA) at 140°C with various stirring speeds of 500, 750, and 1000 rpm. The reaction mixture
then was purified, identified using TLC, and characterized using UV-Vis, FTIR, and 1H-NMR
instruments. Oleic-imidazoline showed a maximum absorption peak at the wavelength of 204 nm
(UV-Vis), absorption at the wavenumber of 1600 and 1500 cm-1 belonging to C=N and C-N-C bonds,
respectively, (FTIR), and a peak at the chemical shift of 2.17 ppm indicating H-C-C=N (1H-NMR).
The performance as corrosion inhibitor from oleic-imidazoline towards carbon steel was conducted
using the weight loss method in 1% (w/v) NaCl solution then compared with a commercial sample.
The highest inhibition efficiency percentage (%IE) of oleic-imidazoline and commercial sample were
86.67% and 80.00%, respectively, at 500 ppm. The %IE value of oleic-imidazoline obtained from
the weight loss method was not significantly different from the electrochemical method (87.95%,
500 ppm). Oleic-imidazoline from this study reveals good performance to inhibit the corrosion
towards carbon steel and is expected to be developed in an industrial scale.
Keywords: imidazoline, oleic-imidazoline, corrosion inhibitor, and carbon steel.

111
Microwave Assisted Synthesis of 4-Methyl Coumarin Derivatives from Various
Dihydroxybenzene
R S Al-Laily1, D U C Rahayu1*, S Handayani1, and H Dianhar2
1
16424, Indonesia
2
Oral Presenter
ABSTRACT
Coumarin is a group of heterocyclic compounds naturally found in plants. Coumarin-derived
compounds have been widely synthesized and some have shown antibacterial activity. On the other
hand, antibiotic resistance of some bacterial strains has urged the research and development of the
new antibacterial compounds. In this study, 7-hydroxy-4-methyl coumarin was successfully
synthesized via Pechmann condensation between resorcinol and ethyl acetoacetate with the presence
of SnCl2.2H2O as catalyst assisted by microwave irradiation. The synthesized compound was
obtained with an optimum yield of 49.85±5.4% by reacting resorcinol/ethyl acetoaceate molar ratio
1:1 (mol/mol) and 10 mol% catalyst for 260s reaction time under solvent-free condition. This
compound was identified by TLC and melting point apparatus then was further characterized using
FTIR, UV-Vis, and NMR (1D and 2D) instruments. Other 4-methyl coumarins derivatives from
catechol and hydroquinone were failed to obtain the desired products which proved that both
dihydroxybenzene have low reactivity compared to resorcinol in Pechmann condensation. This study
reveals that various dihydroxybenzene can affect 4-methyl coumarin synthesis via Pechmann
condensation which the synthesized compounds can be analyzed for its antibacterial activities for
further research.
Keywords: antibacterial, coumarin, dihydroxybenzene, microwave assisted, and Pechmann

condensation

112
Zinc Ferrite Nanoparticles Synthesized Via Hydrothermal Method Using
Simbang Darah Extract as Capping Agent and Their Activities
Rahmayeni*, Rike Febrialita, Yeni Stiadi, Yulia Eka Putri, and Zulhadjri
Chemistry Departement, Andalas University, Padang, 25163, Indonesia
*Email: rahmayenni@sci.unand.ac.id
Oral Presenter
ABSTRACT
This paper reports a Simbang Darah (Iresine herbstii) leaf extract-mediated synthesis of ZnFe2O4
nanoparticles by the hydrothermal method. Biochemical compounds contained in Simbang Darah
leaf extract can act as a capping agent. X-ray diffraction and FTIR confirm the formation of the cubic
spinel crystal structure of ZnFe2O4 nanoparticles. SEM and TEM characterization represents the
morphology of ZnFe2O4 spinel ferrite have a uniform spherical shape in nanoscale. EDX results
showed that the compositional mass ratio were relevant as expected from the synthesis. For the
analysis of DRS UV-Vis, ZnFe2O4 nanoparticles have an absorption edge in the visible region with
Eg in the range of 2.05-2.11 eV. The samples exhibit superparamagnetic behavior from VSM
analysis. Based on the photocatalytic activity was obtained that the ZnFe2O4 nanoparticle indicate
the effective photodegradation on direct red 81 dye with degradation percentage reaches 99.66% after
2 h irradiated under solar light. These results show ZnFe 2O4 stable and come to be a promising
material for dye degradation of photocatalytic under solar light. Moreover, ZnFe 2O4 nanoparticles
show the antibacterial activity on S. aureus and E. coli.
Keywords: ZnFe2O4, hydrothermal, Iresine herbstii, direct red 81, antibacterial

113
Cascade Charge Transfer on Nanostructured BiVO4/TiO2/Dye/Co-Catalyst for
Water Oxidation
Uji Pratomo*1, Indra Purnama2, and Jacob Yan Mulyana3
1
Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Padjadjaran,
Jatinangor, West Java, Indonesia
2
Research Center for Sustainable Indonesia Bangun Indonesia Berkelanjutan Ltd, Pekanbaru, Riau 28289,
Indonesia
3
Department of Applied Chemistry, Graduate School of Urban Environmental Sciences, Tokyo Metropolitan
University, 1-1 Minami-ohsawa, Hachioji, Tokyo 192-0397, Japan
Oral Presenter
ABSTRACT
A titania-coated BiVO4 photoanode surface-modified with ruthenium dye Rutbipy and molecular
water oxidation co-catalyst CuTCPP is fabricated for the first time. The coating of BiVO 4 with
transparent TiO2 nanoparticle and the subsequent deposition of Rutbipy and CuTCPP onto the TiO2
layer improves the photocurrent at the thermodynamic water oxidation potential (0.82 V vs. NHE pH
7) under 160 mW cm−2 illumination. A spectral monitoring of BiVO4/TiO2-Rutbipy film following a
controlled-potential experiment shows the accumulation of Ru3+ formed via hole transfer from BiVO4
to the titania-bound Rutbipy. Hole transfer from Rutbipy to CuTCPP is also evident from a
controlled-potential experiment conducted in the titania-only system. This study provides
experimental evidence and energetic grounds for the cascade hole transfer from BiVO4 to Rutbipy
and to the eventual CuTCPP, which suppress the electron-hole recombination rate and improve the
water oxidation kinetics in the photoanode system.
Keywords: Cascade hole transfer, Water Oxidation, Photo-electrocatalyst, Photo-electrochemistry,

Water Splitting

114
Synthesis Of Zinc Oxide (ZnO) Nanoparticles By Polyol Method And It’s
Application On Photocatalytic Reduction Of Paracetamol Concentration
Anisun Zakiyah 1, Yuly Kusumawati 1,a)
a)
Corresponding author: y_kusumawati@chem.its.ac.id
Oral Presenter
ABSTRACT
Zinc oxide nanoparticles were synthesized by polyol method with diethylene glycol (DEG) as
the polyol. Synthesis was carried out with z = 0,5 mol/L, h ratio = 5 and b ratio = 0. Zinc oxide
nanoparticles were characterized by XRD, FTIR, and SEM Instruments. The characterization
results showed that ZnO nanoparticles were successfully synthesized with hexagonal crystal
structure, average crystallite size from 0,857 to 1,62 nm, round and elongated particles shape,
and particles potentially form nanorods. ZnO nanoparticles were tested for their photocatalytic
activity in reducing the concentration of paracetamol solution under UV-LED irradiation and
without UV-LED irradiation. The biggest paracetamol concentration reduction was 37,9%
under natural initial pH of the solution (pH = 5), the dose of ZnO nanoparticles 0,2 gram in 25
mL of paracetamol 208,4 mg/L, and 3 hours photocatalysis time.
Keywords: Zinc Oxide Nanoparticles, Polyol Method, Photocatalytic, Paracetamol

115
Determination of Several Heavy Metals in Staple Foods from Traditional
Markets in Capital City of Indonesia using Neutron Activation Analysis
Syukria Kurniawatia), Endah Damastutib), Natalia Adventinic),Woro Yatu Niken
Syahfitrid) , Indah Kusmartinie), Diah Dwiana Lestianif) dan Muhayatun Santosog)
Center for Applied Nuclear Science and Technology, National Nuclear Energy Agency, Jl. Tamansari 71
Bandung, Indonesia 40132
a)
Corresponding author: syukria@batan.go.id
b)
endah_dt@batan.go.id, nadventini@yahoo.com, d)woro@batan.go.id, e)indahkus@batan.go.id,
c)
f)
diahdwi@batan.go.id, g)hayat@batan.go.id
Oral Presenter
ABSTRACT
Heavy metals can occur as residues in food because of their presence in the environment, as a result
of human activities such as farming, industry, car exhausts or from contamination during storage.
People can be exposed to these metals by ingesting contaminated food. Their accumulation in the
body can lead to harmful effects over time. The research has been carried out to determine several
heavy metals such as Co, Cr, Hg and Zn in selected foodstuffs from traditional markets in Jakarta, as
the capital city of Indonesia. Determination of heavy metals content was performed using Neutron
Activation Analysis (NAA) method. The range concentration of Co, Cr, Hg and Zn in the foods was
0.01-0.15 μg/g, 0.02-0.49 μg/g, 0.02 – 0.25 μg/g and 1.87-46.2 μg/g respectively. Health risk index
was also determined to assess the toxicity potential of heavy metals in foods, and the results showed
that the selected staple foods from Jakarta are safe for consumption.

116
Antioxidant of Beta-Carotene Emulsion from Red Virgin Palm Oil (RVPO)
Siti Agustina*, Eva Oktarina, Novi Nur Aidha, and Jiwo Hutomo
†
Industrial Research and Development Agency, Ministry of Industry, Republic of Indonesia
*Corresponding author: sitiagustina.rj@gmail.com
Oral Presenter
ABSTRACT
Indonesia is the largest producer and exporter of palm oil in the world. RVPO is an oil palm
derivative containing beta-carotene. Beta-carotene can be used in functional food, cosmetic and
pharmaceutical area. The stability of beta-carotene as antioxidants influenced by temperature,
light, and total oxygen. Stability of beta carotene can be approach by emulsification. The purpose
of this study was to obtain the formulation of beta-carotene emulsions as antioxidants from RVPO
using high pressure homogenation. The process condition used was 30 Mpa for 6 minutes. The
emulsion consists of 6 formulas with the variables used were the type of emulsifier (Tween 80
and CMC) and the ratio of aquadest: RVPO (2:1; 3:1; 4:1). Analysis of research results in the form
of antioxidant activity, emulsion stability, pH, and viscosity. The results show that the best
emulsion was using an emulsifier Tween 80 by comparison 3:1 that give antioxidant activity IC 50
205.95 mg/ml, antioxidant capacity 27.02 mg Vit. C/100 g sample, pH 5.95, and viscosity 110 cp.
The beta-carotene emulsion products can be used as preparations for cosmetics or
pharmaceuticals.
Keywords: emulsion, RVPO, antioxidant

117
Effect of Operating Conditions on The Flux Recovery of Lithium From
Geothermal Brine Using Forward Osmosis
P.C.B.W Mustika1,a), Sutijan1,b), H.T.B.M Petrus1,c)
1
Chemical Engineering Department, Faculty of Engineering, Gadjah Mada University, Sleman, Special
Region of Yogyakarta 55281, Indonesia
a)
pracipta95@mail.ugm.ac.id
b)
Corresponding author: sutijan@ugm.ac.id
c)
bayupetrus@ugm.ac.id
Oral Presenter
ABSTRACT
Along with the development of battery technology, the demand for lithium has increased, but the
availability of lithium from primary ore, the pegmatite, has continued to decline. One of the
alternatives is lithium extraction from brines. The process of lithium recovery from geothermal brine
can be carried out using forward osmosis. In this research, experiments will be carried out in the
laboratory using crossflow module to observe the effect of temperature and concentration on the
resulting flux. The brine geothermal feed was synthesized by mixing NaCl, KCl, LiCl, CaCl 2, and
MgCl2. The concentration of draw solution was varied, namely 5, 2, and 1M, while the temperature
was varied with values of 42, 36, and 30oC. The optimum conditions were obtained at a temperature
of 42oC and a concentration of 5M with a maximum flux of 77.81 LMH.

118
Microwaved-assisted Extraction from Microalgae Chlorella Sp. for Biodiesel
Raw Materials
Lailatul Qadariyah1,a), Donny Satria Bhuana1,b), Yurie Nurmitasari1,c),, Annisaa Rhaudiyah Reza1,d),,
Mahfud Mahfud1,e)
1
Chemical Engineering Department, Institut Teknologi Sepuluh Nopember, Surabaya, 60111, Indonesia.
e)
Corresponding author: mahfud@chem-eng.its.ac.id
a)
lqadariyah@chem-eng.its.ac.id
b)
ds_bhuana@chem-eng.its.ac.id
c)
yuriemitaa@gmail.com
d)
annisaareza@gmail.com
Oral Presenter
ABSTRACT
Microwaved-Assisted Extraction (MAE) is a method that is being developed for extracting a

material, including in extracting microalgae lipids as raw material for producing biodiesel.
However, in its implementation, it is necessary to examine the parameters that affect an extraction
process. Therefore, the method is investigated by studying the effects of extraction time (20; 35;
50; 65; 80 minutes), microwave power (300; 450; 600 watt) and microalgae mass (10; 20; 30
gram), to get maximum yield at extraction process. The first step is to extract microalgae that has
been mixed with H2SO4 then proceed with microwaving by adding NaOH. After extracting, a
centrifuge is carried out to separate the sediment and the supernatant. Then the supernatant is
added with 0.5M H2SO4 and centrifuged again. After that, extract it again by microwave by adding
50 mL of n-hexane. The result is evaporated to form a crystalline precipitate. The precipitate was
given enough methanol and N-hexane to bind oil. Filter the sludge and heat up to the weight of a
bottle containing a constant oil. From the method used to obtain a yield of 88.45% using the mass
of dry 10 gram microalgae at 300 watts of power for 35 minutes. Based on these results, it can be
said that the extraction process with Microwaved-Assisted Extraction is an efficient method
because it can save time and energy because the extraction occurs simultaneously. Furthermore,
the time of extraction, microwave power and microalgae mass will increase the opposite effect in
obtaining lipids (fewer lipids). This indicates that the three parameters are inversely proportional
in producing large lipids. In addition, the obtained lipids have 36.20% palmitic acid which has the
potential to be easily converted to biodiesel.
Keywords: Biodiesel, Chlorella Sp., Microalgae, Microwave Assisted Extraction.

119
Kinetics Study of Microwave Hydrodestillation (MHD) Fresh and Dried
Cymbopogon Nardus Leaves
Zuhdi Ma’sum1,a), Donny Satria Bhuana1,b), Mahfud Mahfud1,c)
1
Chemical Engineering Department, Institut Teknologi Sepuluh Nopember, Surabaya, 60111, Indonesia.
c)
Corresponding author: mahfud@chem-eng.its.ac.id
a)
zuhdimasum49@chem-eng.its.ac.id
b)
ds_bhuana@chem-eng.its.ac.id
Oral Presenter
ABSTRACT
Extraction citronella oil from Cymbopogon Nardus using Microwave Assisted Hydro Destillation
(MAHD) method using water as solvents in the process. MAHD is an alternative technique and
method to produce citronella oil and it has several advantages in terms of product quality and short
time process. In this study, the citronella oil was extracted from Cymbopogon Nardus leaves (fresh
and dry) used extraction time from 10 to 90 min and microwave power in the range at 300-600
W. The results of citronella oil extraction using the microwave hydro distillation method shows
that the yield of citronella oil obtained will increase rapidly at the beginning and then decrease
with the length of the extraction time. The yield obtained using MAHD could produce the highest
yield at 450 W and 90 min are 0.2636 g oil/100 g lemongrass dry leaves. In addition, the kinetic
study of MAHD model appears to be the best fit to explain with second-order than first-order
model. It would be proved by good correlation between the experimental data and model achieved
(R2>0.99) and (RMSE=0.0021). Based on the results obtained, second-order kinetic models could
actually describe the kinetics model of extraction process citronella oil from Cymbopogon Nardus
using MAHD.
Keywords: Citronella oil, Cymbopogon Nardus, Microwave Assisted Hydro Destillation, kinetic
modeling.

120
Chemical Pre-treatments Effect for Reducing Lignin on Cocoa Pulp Waste for
Biogas Production
Tri Widjaja,*) Siti Nurkhamidah, Ali Altway, Aisyah Alifatul Zahidah Rohmah, Ferdi Saepulah
Chemical Engineering Departmet, Faculty of Industrial Technology and Systems Engineering, Institut
Teknologi Sepuluh Nopember (ITS), Kampus ITS Sukolilo, Surabaya 60111 Indonesia
Corresponding author: triw@chem-eng.its.ac.id
Oral Presenter
ABSTRACT
There are many cocoa pulp waste in Indonesia; unfortunately, still a few of them has been utilized.
Cocoa pulp contains high enough of cellulose and hemicellulose that can be converted to reducing
sugar by using hydrolisis process. However, it also has immense lignin content that inhibits the
enzymatic hydrolysis process. How to reduce lignin contents will be studied in this research by
comparing pretreatments using NaOH solution and using NaOH-H2O2 mixture solution. Lignin,
hemicellulose, and pectin contents were analyzed by using UV Vis spectrophotometer and
gravimetric method was used to analyze cellulose contents. Morphology, and crystallinity index (CrI)
were analyzed by using Scanning Electron Microscope (SEM), and Xray Diffraction Pattern (XRD),
respectively. The experimental results showed that pretreatment with NaOH-H2O2 was the best
method with a decreasing in lignin content up to 85.67%. This result is supported by SEM results
showing that with the presence of pretreatment, the previously smooth surface of cocoa pulp becomes
rough surface and many void/cracks. XRD results show that the CrI of cocoa pulp waste decrease
after being chemically pretreatments, especially in NaOH-H2O2 pretreatments. COD removal with
NaOH-H2O2 is the highest than others. TS and VS decrease with the increasing of fermentation time.
Contribution of acetic acid in VFA total is higher than others and achieved maximum value after 15
days and decrease rapidly after 20 days and being constant after 25 days of fermentation. This result
agrees with biogas production and resulting methane with concentration of 66.07% and yield of 0.734
m3 CH4/kg VS.

121
Application of The Multi Soil Layering (MSL) System to Treat Laboratory
Wastewater
Salmariza Sy(1), Sofyan(1), Ardinal(1), Inda Trhee Anova(1), Hasni M(1), Marlusi(1), Tsugiyuki
Masunaga(2), Toshiyuki Wakatsuki(2,3
1
1Institute for Research and Standardization of Industry Padang, Indonesia
2
Faculty of Life and Environmental Sciences, Shimane University, Matsue 690‐8504, Japan
2,3
Faculty of Agriculture, Kinki University, Nara 631 ‐8505, Japan
1)
rizasalma@gmail.com
1)
sofyantk84@gmail.com
1)
ardinal_ok@yahoo.com
1)
indova99@gmail.com
1)
Hasnimustaf40@gmail.com
1)
lucyalaric23@gmail.com
2)
masunaga@life.shimane-u.ac.jp
2,3)
wakatuki@nara.kindai.ac.jp
Oral Presenter
ABSTRACT
Laboratory wastewater must be managed and handled properly so as not to damage the
environment. The management was carried out starting from the separation and labeling of the
wastewater according to its character, to processing it with a wastewater treatment plants
(WWTP). The chemical waste resulting from the analysis which was classified as hazardous (B3)
wastewater was separated and processed by a third party who has the authority, while the
wastewater from washing glass utensils from the sink was channeled to the laboratory wastewater
treatment plants (WWTP). The application of the Multi Soil Layering (MSL) system to treat
laboratory wastewater from the Institute for Research and Standardization of Industry Padang was
carried out by making a 2 x 3 x 2meter sedimentation tank, two 3 x 3 x 2.5meter MSL system
tanks, two 0.5 x 0.6 x 0.5 meters control tanks and a 2 x 3 x 1 meter tank for a fish pond. The
processing results revealed that the WWTP with the MSL system was effectively used to treat
wastewater from that laboratory. The analysis results showed a high reduction efficiency which
was able to reduce the pollutant parameters BOD, COD, TSS, Fatty Oil and Ammonia 95%, 88%,
89%, 64%, and 81%, respectively, which had met quality standards, and could reduce metal ions
that were still present in laboratory wastewater.

122
Immobilization of Trypsin onto Porous Monolith Methacrylate on High-
Performance Liquid Chromatography Applications
Suci Amalia1, 2, Dias Septiana1, Stevin Carolius Angga1, Elvina Dhiaul Iftitah1, Warsito1, Akhmad
Sabarudin1,3*
1
Department of Chemistry, Faculty of Science, Brawijaya University, Malang 65154, Indonesia.
2
Department of Chemistry, Faculty of Science and Technology, Maulana Malik Ibrahim Islamic State
University, Malang 65144, Indonesia.
3
Research Center for Advanced System and Material Technology (ASMAT), Brawijaya University, Malang
65145, Indonesia
*)
Corresponding author: sabarjpn@ub.ac.id
Oral Presenter
ABSTRACT
Column technology involving various materials and efficient reactions has attracted the attention of
researchers, especially in the manufacture of monolithic columns for analytical applications. In this
work, the laboratory-made organic-polymer monolithic column is modified with trypsin and further
applied as a nanobiocatalyst microreactor and a stationary phase for separating chiral compounds by
HPLC. The monolith was synthesized by in-situ copolymerization of glycidyl methacrylate (GMA)
and ethylene glycol dimethacrylate (EDMA) or trimethylolpropane trimethacrylate (TRIM) as a
crosslinking agent, with porogen of propanol/1,4-butanediol/H2O (7:4:1 v/v) and AIBN (1%, w/v) as
the radical polymerization initiator inside PEEK and silicosteel tubings (1.0 i.d x 100 mm) at 60 oC
for 12 h. A total monomer ratio (%T) and crosslinking agent (%C) of 40:25 and 28:12 were applied
to prepare poly-(GMA-co-EDMA) and poly-(GMA-co-TRIM), respectively. The produced
monoliths were further modified by introducing trypsin (10 mg/L) through the ring-opening reaction
of the epoxide group existing in the monolithic column. The monolithic poly-(GMA-co-EDMA)-
trypsin column was successfully applied as the nanobiocatalyst microreactor for the rapid and
efficient digestion of β-casein sample into its peptide fragments. The monolithic poly-(GMA-co-
TRIM)-trypsin column was used as the HPLC stationary phase for the separation of R/S-citronellal
enantiomers.
Keywords: monolith, protein, trypsin, citronellal, enantiomer, nanobiocatalyst

123
The Combination of Natural Dyes from Jengkol Pod Waste (Archidendron
jiringa) and Liquid Waste of Gambier (Uncaria gambir Roxb) on Coloring of
Cotton Fabrics
S. Sofyan1,a), F. Failisnur2b), and S. Silfia3c)
1
Balai Riset dan Standardisasi Industri Padang
Jalan Raya LIK No. 23 Ulu Gadut Padang, Indonesia
2
3
a)
Corresponding author: sofyantk84@gmail.com
b)
failisnur@gmail.com
c)
silfiabintiarsul@gmail.com
Oral Presenter
ABSTRACT
Natural colors for textile products are becoming a trend today as an alternative to reducing the
use of synthetic dyes. Jengkol pod waste is a useless resource and is disposed as waste.
Likewise with the gambier liquid waste which is produced from the pressing process of the
gambier leaves. Both of these resources contain tannins which can function as dyes. The use
of these two natural resources as natural dyes is an effort that can increase added value. The
research was aimed to color and see the quality of the dyed cotton fabric using a combination
of jengkol pod waste and gambier liquid waste. The research was conducted by extracting the
dye of the jengkol pod waste and gambier liquid waste, and testing the application of the dye
on cotton fabrics. Three types of mordants, namely Al2(SO4)3, CaO, and FeSO4 were used in
the coloring process. Treatment without mordant (control) was carried out as a comparison.
Cotton fabrics that had been dyed were tested for the quality of their coloring by testing the
color coordinate, color strength, and color fastness. The results showed that each mordant gave
a different color coordinate with brown color in the use of mordant CaO and mordant
Al2(SO4)3, and greenish gray color with mordant FeSO4. The highest color strength was
obtained in the use of mordant FeSO4 with K/S value of 11.678. Treatment with mordant CaO
was the best treatment in terms of color fastness of washing, acid sweat, alkaline sweat, light,
and rubbing with values of 4-5, 4-5, 3, 4, 4-5 respectively.

124
Isolation and Molecular Docking Study of A Novel Ergone Steroid from
Endophytic Fungus Aspergillus Carbonarius of Sponge Aaptos Suberitoides
Muhammad Syahrur Ramadhan, Reza Aditama,
Euis Holisotan Hakim
Bandung Institute of Technology, Indonesia
Muhammadsyahrur2@gmail.com
Oral Presenter
ABSTRACT
The discovery of lead compounds has shifted from the terrestrial to the marine organisms as they are
suspected to produce various important metabolites. Sponge-derived endophytic fungi are promising
sources of structurally novel and biologically active compounds. This study aims to isolate and
investigate the biological activity of secondary metabolites from endophytic fungi associated with
sea sponge Aaptos suberitoides. Endophytic fungi from sea sponge Aaptos suberitodes were
inoculated on malt extract agar with chloramphenicol 0.4% and cultivated on rice solid media at 28 0C
for its optimum cultivated time. The endophytic fungus from sea sponge Aaptos suberitodes was
identified molecularly as Aspergillus carbonarius. In this ongoing research, an ergone steroid
compound, (22E,24R)-ergosta-4,6,8(14),22-tetraen-3-one, was isolated from the EtOAc extract of
the endophytic fungus A. carbonarius. The structure was elucidated by spectroscopic methods
including 1D and 2D NMR techniques. This isolated steroid compound was the first reported in the
species A. carbonarius. In addition, molecular docking was carried out for the compound against the
active site of EGFR tyrosine kinase and Tymidilate synthase, targeted enzymes for colorectal cancer.
The molecular docking study successfully calculated the binding energy value respectively -9.7
kcal/mol and -10.6 kcal/mol and expressed the interaction between the isolated ergone steroid and
the active site of the enzymes. It is indicated that 22E,24R)-ergosta-4,6,8(14),22-tetraen-3-one has
great potential to inhibit the colorectal cancer cells.
Keywords: Endophytic, Aspergillus, Aaptos, Steroid, Docking

125
Optimization of Lignin Removal Pre-Treatment Process in Acetone-Butanol-
Ethanol (ABE) Production from Empty Fruit Bunches of Oil Palm
Lily Pudjiastuti1*, Tri Widjaja2, Ali Altway2, Inti Rohmania1, Nur Azizatur Rohmah1
1
Departement of Industrial Chemical Engineering, Sepuluh Nopember Institute of Technology Surabaya,
2
Departement of Chemical Engineering, Sepuluh Nopember Institute of Technology Surabaya, Sukolilo,
Surabaya 60111, Indonesia
*E-mail : lily_p@chem-eng.its.ac.id
Oral Presenter
ABSTRACT
Empty Fruit Bunch (EFB) is an abundant waste from palm oil refinery. Its high lignocellulose content
can be utilized as raw material of Acetone-Butanol-Ethanol (ABE). However, lignin content become
a hindrance in enzymatic hydrolysis and fermentation process. Alkaline Hydrogen Peroxide (AHP)
Pre-treatment was employed in this study to remove the lignin since it has high selectivity. The
optimum operating condition (EFP and AHP ratio, temperature, and time) was also studied using
Response Surface Methodology (RSM). The lignin removal was significantly influenced by the
operating condition based on Analysis of Variance (ANOVA) (P≤0.05). The highest removal of
lignin (91.6%) was obtained at 54,56 ⁰C, 20% ratio of EFB in AHP and 6 hr. The highest 97.75% of
lignin removal was attained from developed model at 43.57 ⁰C, 30.86% ratio and 6.75 hr.
Keywords: Empty Fruit Bunch, Alkaline Hydrogen Peroxide, Optimization, Response Surface
Methodology,

126
Computational Study of Nickel Doped ZnO Cluster for Cationic and Anionic
Dye
Nanik Ismi Oktavianti1, Garcelina Rizky Anindika1, Atthar Luqman Ivansyah2, Hendro Juwono1,
and Yuly Kusumawati1, a)
1
Department of Chemistry, Institut Teknologi Sepuluh Nopember, Surabaya, Indonesia
2
Department of Chemistry, Institut Teknologi Bandung, Bandung, Indonesia
a)
Corresponding author: y_kusumawati@chem.its.ac.id
Oral Presenter
ABSTRACT
In this study, nickel (Ni) doped ZnO cluster was used to evaluate adsorption interactions of
cationic (methylene blue) and anionic (methyl orange) dyes. They have been investigated
through DFT calculations by ORCA program. The interaction between dye and Ni-doped ZnO
has been studied in terms of energy gap (Eg), bond length, adsorption energy (Eads), and
Mulliken charge transfer to examine the sensitivity of Ni-doped ZnO towards cationic and
anionic dyes. It was found that Zn12O12 was more sensitive to adsorb MB than Ni-doped
Zn12O12, meanwhile MO molecule has strong interaction on Ni-doped Zn12O12 cluster.

127
Calculations of Linoleic and Linolenic Acid Esterification Using ZnO Catalyst
DFT
Anas Fahmi Imron1, Yuly Kusumawati1, Atthar Luqman Ivansyah2, Hendro Juwono1,*
1
Department of Chemistry, Institut Teknologi Sepuluh Nopember , Indonesia
2
Department of Chemistry,Institut Teknologi Bandung, Indonesia
*
E-Mail / Contact Details : hjachmad@gmail.com
Oral Presenter
ABSTRACT
DFT calculations were performed to study esterification mechanism of linoleic acid and linolenic
acid with methanol, as model reactions for biodiesel production using ZnO catalyst. ZnO is known
as a good photocatalyst which can also be employed for this purpose. The computations were
conducted using Orca version 4.2 with LANL2DZ basis set to obtain transition state (TS) projections
of the esterification, as well as their energies. The uncatalyzed and catalyzed reactants were modelled
to compare and see the effects of catalysis. The simulations show that the presence of ZnO catalyst
not only provides adsorption site but also activates the species and consequently eases the reaction
mechanism.
Keywords: ZnO, chemical computation, DFT, transition states

128
The Effect of Stirring and Fermentation Time of Coconut Cream on The Yield
and Quality of Virgin Coconut Oil (VCO)
Ita Ulfin, Harmami, Dewi Kurnia and Hendro Juwono,
Sepuluh Nopember Institute of Technology, Indonesia
hjachmad@gmail.com / 08155040488
Oral Presenter
ABSTRACT
The quality of Virgin Coconut Oil (VCO) has been conducted to study the effect of stirring and
fermentation time of coconut cream on yield using natural and bread yeast (S. cereviceae)
fermentation methods. The stirring and the fermentation time have been variation. The quality of
VCO were calculated as yield, according to SNI 7381: 2008 and APCC (Asian Pacifics Coconut
Community). The results showed that the highest VCO yield was found in the natural fermentation
method with a stirring time of 20 minutes and then 24 hours of fermentation, that is 26.63%, for the
bread yeast fermentation and 20.13 % for the 28 hours of fermentation without stirring. In both
methods, the longer fermentation and the stirring time of coconut cream, should be the higher and
the yield produced of VCO.
Keywords: Virgin coconut oil, fermentation, quality of VCO

129
Annealing Treatment for Enhancing of H2/C3H8 Separation Performance on
Polysulfone Membrane
Irmariza Shafitri Caralin1, Alvin Rahmad Widyanto1, Nurul Widiastuti1,a, Rika Wijiyanti1, Triyanda
Gunawan1, Zulhairun Abdul Karim2,3, Mikihiro Nomura4, Yuki Yoshida4
1
2
Advanced Membrane Technology Research Center (AMTEC), Universiti Teknologi Malaysia, 81310 UTM
Skudai, Johor Darul Ta’zim, Malaysia
3
Department of Energy Engineering, School of Chemical and Energy Engineering, Universiti Teknologi
Malaysia, 81310 UTM Skudai, Johor Darul Ta’zim, Malaysia
4
Department of Applied Chemistry, Shibaura Institute of Technology, 3-7-5 Toyosu, Koto-ku, Tokyo 135-
8548, Japan
a)
Oral Presenter
ABSTRACT
Recovery of heavier hydrocarbon gas (i.e. C3H8) from permanent gas (i.e. H2) during gas
streams process is a great importance economically. Separation of H 2/C3H8 gas using
polysulfone (PSF) polymeric membrane was studied with the effect of post-treatment process
such as annealing to improve the gas separation performance. PSF membrane annealed using
a muffle furnace under vacuum conditions at 150°C for an hour. The result showed that
annealing process caused a reduction in the mobility of the polymer chain, which was indicated
by a shift of 2θ in XRD results. The cross-section morphology of membrane annealed at 150°C
was examined by SEM. Annealing process of PSF membrane enhanced the H 2/C3H8 selectivity
by 619.77%, from 3.08 to 22.19. It was concluded that post-treatment PSF membrane could
improve the H2/C3H8 separation performance compared to PSF membrane without anneling
treatment.
Keywords: Polysulfone, Polymeric Membrane, Gas Separation, Annealing, H2/C3H8

Selectivity.

130
Synthesis and Characterization of Thermosensitive-Ionic Based Gel in Various
Temperatures
Eva Oktavia Ningrum1, Suprapto1, Achmad Dwitama Karisma1, Ely Agustiani1, Hikmatun Ni’mah2
1
Department of Industrial Chemical Engineering, Faculty of Vocational Studies, Institut Teknologi Sepuluh
Nopember, Kampus ITS Sukolilo, Surabaya, 60111, Indonesia
2
Department of Chemical Engineering, Faculty of Industrial Technology, Institut Teknologi Sepuluh
Nopember, Kampus ITS Sukolilo, Surabaya 60111, Indonesia
1)
Corresponding author: eva-oktavia@chem-eng.its.ac.id
Oral Presenter
ABSTRACT
The rapid development of technology in industry produces heavy metals waste which belongs to B3
waste, thus management is needed to maintain the ecosystem and life around it. One of the industries
which needs B3 waste management is electroplating industry containing metal elements, one of them
is chromium. One of the methods used to regenerate the heavy metals waste is by utilizing zwitterion
betaine gel. However, the weakness of this method is the presence of intra-intra chain association so
that the ionic group ability to adsorb the ion is inhibited by the charge group interaction. Therefore,
in this research, the intra-intra chain of these gels were minimalized by synthetizing anionic and
cationic gel separately using NIPAM-co-Chitosan and NIPAM-co-Acrylic Acid copolymer. NIPAM-
co-Chitosan and NIPAM-co-Acrylic Acid gels were synthetized through polymerization reaction
using N,N'-methylenebisacrylamide (MBAA) as the cross linker with NIPAM-co-Chitosan and
NIPAM-co-Acrylic Acid concentration ratio of 9:1, 8:2, 7:3, and 6:4 at temperature of 10 oC. Heavy
metal adsorbed simultaneously by the charge group in gel and desorbed by NIPAM thermosensitive
ability. Several analysis were done to study the characteristic of the gel produced, including FTIR,
NMR, swelling degree, adsorption ability and gel morphology through SEM. Based on the FTIR
analysis result on NIPAM-co-Chitosan and NIPAM-co-Acrylic Acid gels, only a few vinyl group
seen on its functional group, so that it can be summed up that the synthesis of the gel copolymer
carried out was successful. The highest swelling degree on NIPAM-co-Chitosan was at synthesis
temperature of 10 oC, while on NIPAM-co-Acrylic Acid was at synthesis temperature of 30 oC. The
lowest swelling degree on NIPAM-co-Chitosan was at synthesis temperature of 70 oC, while on
NIPAM-co-Acrylic Acid was at synthesis temperature of 70 oC. Furthermore, based on SEM result,
porous was found on both gel at synthesis temperatures of 30 oC, 50 oC, and 70 oC.

131
Study on Electrochemical Analysis of Uric Acid using Screen Printed Electrode
(SPE) Modified Co, Ni, and Co/Ni
Aldi Wahyudin1, Rahmat Wibowo1, a), and Dita Arifa Nurani1
1
a)
Corresponding author: rahmat.wibowo@sci.ui.ac.id
Oral Presenter
ABSTRACT
Abnormal uric acid levels in the blood and urine cause various diseases so that a fast uric acid
detection is needed. The use of sensors to detect uric acid electrochemically using a Screen Printed
Electrode (SPE) has been carried out. This research was conducted to detect uric acid level with
precision. Measurement of the active surface area of the electrodes was measured using a cyclic
voltammetry with an active electrode surface area of 87.16 %. Modification of the electrodes was
carried out using the chronoamperometric technique of Co, Ni, and CoNi metals at a fixed
potential in sequence of -0.9 V; -0.8 V; -0.9 V for 180 seconds. The SPE modification was
characterized using Scanning Electron Microscopy / Energy Dispersive X-Ray Sprectroscopy
(SEM / EDS). The optimum pH obtained is pH 7 for testing uric acid using bare SPE and has a
linearity value of 0.9109. The results of the linearity test, LOD value, and sensitivity obtained
from the modification of the electrodes Co / SPE, Ni / SPE, and CoNi / SPE against testing of uric
acid were 0.9185, 3.02 x 10-3 M, and 2049.4 µA mM-1 cm-2 for Co / SPE; 0.9923, 0.169 x 10-3 M,
and 9874 µA mM-1 cm-2 for Ni / SPE; and 0.989, 2.34 x 10-3 M, and 3563,8 µA mM-1 cm-2 for
CoNi / SPE.
Keywords: Co/SPE; Cyclic Voltammetry; Ni/SPE; Screen Printed Electrode; Uric Acid

132
Synthesis And Phase Transformation Of Hydroxyapatite From Indonesian
Natural Sources
Kristanto Wahyudi1, a), Herlina Damayanti1, Ria Juliana Manullang1, Ayu Ratnasari1, Nurhidayati2,
and Anita Yulianti3
1
Center for Ceramics, Ministry of Industry, Bandung, West Java, Indonesia
2 Institution of Research and Standardization of Industry Banjarbaru, South Kalimantan, Indonesia
3 Faculty of Dentistry, Airlangga University, East Java, Indonesia
a)
Corresponding author: kristantowahyudi@gmail.com
Oral Presenter
ABSTRACT
Hydroxyapatite (HAp) is an essential material for human bone growth. Several methods of
hydroxyapatite synthesis that have been developed are hydrothermal, mechanochemical, and wet
precipitation methods. Synthetic Hydroxyapatite applications may vary widely from bone repair,
metal implant coating to artificial bone. This study used Padalarang lime and food grade phosphoric
acid 85%. Wet precipitation method was proposed based on calculated the stoichiometric molar ratio
of Ca/P corresponding for Hydroxyapatite and followed by heat treatment from 600 oC, 700oC, 800oC,
900oC, 1000oC, 1100oC, 1200oC and 1400oC. Structural characterization and thermal analysis were
done using X-ray diffraction (XRD) and TGA/DTA. The XRD results showed that HAp undergoes
phase transformation. Heat treatment from 600 oC – 900oC gave slowly crystallization on HAp. At
temperature 1100oC, XRD pattern showed the similar crystalline with natural HAp. At temperature
1200oC and 1400oC, XRD pattern slightly appeared OCP crystalline. The result of TGA/DTA
showed endothermic reactions at temperatures of 165.6 oC, 264.2oC, 359.5oC and 446.8oC
respectively and an exothermic reaction of 446.8 oC to 1200oC. The largest mass loss was 6.16%
during heat treatment. Wet precipitation synthesis is the simple method to produce Hydroxyapatite
from Indonesian natural sources. Variations of heat treatment give affected on phase transformation
from amorphous HAp to crystalline HAp.

133
Adsorbent From Bagasse To Trap The Red Procion Substance In Batik Waste
Camelia Amsa Fahira1), Dewi Riana Damayanti1), Alifia Febrianti1), Retno A.S. Lestari2, 3)
1
Student of Chemical Engineering Department, University of 17 Agustus 1945 Semarang
2
Lecturer of Chemical Engineering Department, University of 17 Agustus 1945 Semarang
3
Study Center for Environmental and Energy Alternative 17 Agustus 1945 University, Semarang, Indonesia
a)
cameliafahira@gmail.com
Oral Presenter
ABSTRACT
The batik and textile industry is one of the producers of liquid waste from the coloring process.
Liquid waste in the batik coloring process is very dangerous. There are several methods of
processing batik liquid waste, one of which is the adsorption method using activated carbon
adsorbent. In this research, activated carbon is made from bagasse which is activated with NaCl.
The chemical composition of bagasse is 50% cellulose, 25% hemicellulose, and 25% lignin. The
purpose of this experiment was to determine the process of making activated carbon adsorbent
from bagasse and to determine the absorption rate of bagasse adsorbent against red procion dye
in batik industrial wastewater. Research on the use of activated carbon from bagasse to absorb red
procion dye from batik industrial waste has been carried out. Activated carbon was made by
carbonization process at 4000C for 30 minutes. The activated carbon produced was carried out by
FTIR characterization to determine its functional groups. The optimum conditions for the
adsorption of activated carbon from bagasse against red procion were carried out by variable
contact time, weight of activated carbon, and pH. The results of FTIR characterization on activated
carbon from bagasse have -CO- and -OH functional groups. The optimum conditions for
adsorption of activated carbon from bagasse were obtained at a contact time of 90 minutes with a
weight of 0.1 g of activated carbon and an optimum pH of 5 at 50 mL of red procion dye with a
concentration of 20 mg / L. The absorption effectiveness of red procion dye from batik industrial
wastewater by activated carbon from bagasse in optimum conditions is 69, 04%.

134
Modification of Gold Electrodes Using 3-Mercaptopropionic Acid and EDTA as
a Copper Sensor Application.
Mila Rahma Minanti Putri, Rahmat Wibowo, Dita Arifa Nurani
Department of Chemistry, Faculty of Mathematics and Natural Sciences (FMIPA),
University Of Indonesia, Indonesia
Milaputri35@gmail.com/ 087877235016
Oral Presenter
ABSTRACT
Modification of the gold electrode is an alternative method that can be used as a

chemical sensor for the analysis of Cu2+ metal ions. Modification of the gold electrode
was carried out by immobilizing 3-mercaptopropionic acid on the surface of the gold
electrode using the self assembled monolayer (SAM) method which was functionalized
with EDTA. Gold electrode characterization was performed by cyclic voltametry (CV),
fourier-transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM)
and energy dispersive spectrometer (EDS) techniques. The electrode area used is 0.809
cm2. Modifications and applications of Au-Bare electrode sensors, Au-3MPA and Au-
3MPA-EDTA against the metal ion detection of Cu 2+ have been successfully
performed. The top of the current Cu2+ metal ion oxidation state is found in pH 5 among
others, 8.07 x 10-5 A on Au-3MPA-EDTA electrodes, 5.92 x 10-5 A on Au-3MPA
electrodes and 7.42 x 10-5 A on Au-Bare electrodes at a potential range of 0.35 V.
Keywords: Gold electrodes; 3-mercaptopropionic acid; self assembled monolayer;

EDTA; cyclic voltammetry.

135
Functionalization Single-Walled Carbon
Nanotubes with a HNO3/H2SO4 Mixtures Through Different Treatments: A DFT
Supported Study
Herbi Yuliantoro1 b , Aswati Mindaryani2, Murni Handayani3, and Yuni Kusumastuti 4 a
1,2,4
Chemical Engineering Department, Faculty of Engineering, Gadjah Mada University, Indonesia
3
Research Center for Metallurgy and Materials, Indonesian Institute of Sciences.
a)Corresponding author: yuni_kusumastuti@ugm.ac.id
b) herbi.yuliantoro@mail.ugm.ac.id
Oral Presenter
ABSTRACT
Chemical functionalization of Single-Walled Carbon Nanotubes (SWCNT) is carried out to

improve the surface interaction capability when it is used in its end application such as drug
carriers. This research compares the ultrasonic bath and stirring process. Both methods were
conducted at room temperature. The functionalizations were performed by two different solvents
which are nitric acid and a mixture of sulfuric acid and nitric acid (3:1). The result of Fourier
Transform Infrared (FTIR) spectra shows the appearance of carboxyl group at 1730 cm-1 in
the sonication with mixed acid. Acidic content results by Boehm titration show that new acid
groups were attached. Both analyzes indicated that the –COOH group was successfully attached
to the SWCNT. The study of density functional theory (DFT) was used to support the experimental
results. DFT results show that increasing of the carboxyl group will improve the stabilization.
Keywords: SWCNT, Functionalization, Sulfuric Acid, Nitric Acid, DFT

136
Dry Mechanosynthesis and Characterization of Carbonate Apatite Based on
Indonesian Natural Sources
Herlina Damayanti1, a), Kristanto Wahyudi1, Karlina Noordiningsih1, Ayu Ratnasari1, and Devi
Rianti2
1
Center for Ceramics, Ministry of Industry Republic of Indonesia
2
Faculty of Dentistry, Airlangga University, Indonesia
a)
Corresponding author: herlinadamayanti49@gmail.com
Oral Presenter
ABSTRACT
Synthetic carbonate apatite is widely used in bone tissue engineering because of their similar
chemical composition with the inorganic component of hard tissue. In natural hard tissue, apatite is
supplemented by tracing ions such as carbonate. Carbonate apatite more bioactive than stoichiometric
hydroxyapatite. In this study, carbonate apatite powders were prepared by dry mechanosynthesis
method, the powder mixture of hydroxyapatite (HA) powders from Center for Ceramic based on
Indonesian limestone and carbonate sources came from CaCO 3 (Cirebon origin) and MgCO3. The
synthesized results were characterized by X-ray diffraction (XRD), X-ray fluorescence (XRF),
Fourier-transform infrared spectroscopy (FTIR), and scanning electron microscopy (SEM). The dry
mechanosynthesis method produced carbonate apatite, which is formed by solid to a solid reaction
between hydroxyapatite and carbonate sources. The XRD patterns peaks around 31,6 - 33o confirmed
the formation of the apatite phase. Based on FTIR spectra, the mixture of HA and MgCO 3 forms type
B of carbonate apatite as predominant and type A is not dominant. SEM image shows that the
particles have an irregular shape with agglomerates interconnected between fine particles formed
large particles. Dry mechanosynthesis is the right and simple method to produce carbonate apatite
from Indonesian natural sources. This method can reduce waste and cost during production.
Keywords: carbonate apatite, hard tissue, dry mechanosynthesis, Indonesian natural sources, solid
to solid reaction

137
Isolation, Selection and Identification of Sulphur Oxidizing Bacteria (SOB) from
Sludge of Tofu Industry Wastewater
Retno A.S. Lestari
University of 17 Agustus 1945 Semarang, Indonesia
retnotengaran@gmail.com
Oral Presenter
ABSTRACT
Alternative energy sources to substitute fossil fuel has been developed, and one of them is biogas.
However the content of H2S in biogas needs to be removed, because it promotes corrosion in the
equipment utilizing biogas. The elimination of H2S from biogas can be done using biological
processes. Elimination of H2S was performed by biofiltration, in which H2S degrading bacteria
immobilized on the packing material inside a column. Bacteria was taken from the local area, while
salak fruit seed was used as packing material. Biofilm-forming bacteria isolated from sludge was
taken from liquid wastewater treatment that convert tofu industry waste into biogas. To determine
the ability of salak fruit seeds as a packing material, it was performed biofilm bacteria immobilization
test that taken from the sludge to surface of salak fruit seeds. After 2 days it was observed that salak
fruit seed surface was covers with bacteria. From the results of immobilization of bacteria taken from
the sludge 15 isolates were obtained. From the test to the 15 isolates in degrading sulfide, it was
showed that all isolates have capability to degrading sulfide but with different abilities. Then 3 best
isolates were selected, namely isolates 7, isolates 11 and isolates 12, to be re-tested their capability
on forming biofilm on the surface of the salak fruit seeds. It was observed that isolates 12 is the best.
Further identification showed that isolate 12 was Bacillus cereus with the similarity value of 98%.
Keywords: Bacillus cereus, Biogas, Biofilm, Elimination, H2S

138
Adsorption of Mercury (II) From Aqueous Solution By Crumb Rubber Sludge:
Isotherm and Kinetic Studies.
Monik Kasmana1, Hadraha, Titania Eno Widyaningsiha, Salmariza Syb
a
Department of Environmental Engineering, Faculty of Engineering, University of Batanghari, Jambi,
Indonesia
b
Institute for Research and Standardization of Industry Padang, Indonesia
1
Corresponding author: monik.kasman@unbari.ac.id
Oral Presenter
ABSTRACT
The use of two different crumb rubber adsorbent (CRS), pure crumb rubber sludge (pCRS) and
modified crumb rubber sludge (mPCRS) with HNO3 activator was investigated for the removal of
Hg(II) from aqueous solution. Batch experiment was conducted to analyze the effect of adsorbent
dosage and contact time on the adsorption capacities of pCRS and mCRS. Adsorption isotherm and
adsorption kinetics was also analyzed to get description of the adsorption mechanism and the
adsorbent properties. The Langmuir isotherm provided the best correlation with the maximum
adsorption capacity was 16.00 mg/g an, 17.513 mg/g for respected to pCRS and mCRS. The kinetics
studies showed that the Hg(II) for both adsorbents adsorbed rapidly which can be adjusted to the
pseudo second order model. Our current study confirmed that CRS was effective adsorbent for the
removal of Hg(II) from aqueous solution which followed monolayer and multilayer chemisorption.

139
Alkali Metal Doped ZnO Nanoclesters for Dye Photodegradation:
Computational Studies
Garcelina Rizky Anindika a, Nanik Ismi Oktaviaa, Atthar Luqman Ivansyahb, Arif Fadlana, Yuly
Kusumawatia,*
a
Department of Chemistry, Institut Teknologi Sepuluh Nopember, Indonesia
b
Department of Chemistry, Institut Teknologi Bandung, Indonesia
*e-mail: y_kusumawati@chem.its.ac.id
Oral Presenter
ABSTRACT
ZnO is n-type metallic due to the zinc interstitials and oxygen vacancies. In order to improve its
applications, p-type doped ZnO is needed. For a good dopant, it should have appropriate solubility
and ionization energy. As we know, alkali have good electrical and thermal conductivity and
influence the properties of semiconductor nanoclusters (NCs) which help to achieve the desired
applications. The computational methods have been used to study the electronic properties of Zinc
Oxide (ZnO) nanoclusters dopped of several alkali. It has been found that doping processes of the
alkali metals are endothermic. Based on the results, alkali metal doping sensitive to ZnO nanocluster
so that it is transformed from intrinsic semiconductor with HOMO–LUMO energy gap of 3.77 eV to
an extrinsic semiconductor with the energy gap of ~1.11–1.95 eV. The doping of alkali metals
increases the work function of the ZnO which may influence the electron emission from the tube
surface.
Keywords: ZnO, Computational, Dopping, Alkali, Photodegradation

140
Non Polar Extracts from Stem Bark of Garcinia lasoar against Staphylococcus
aureus
Healthy Kainama1,2, Sri Fatmawati2, Mardi Santoso2, Adi Setyo Purnomo2, Hamdan Dwi Rizqi2,
Taslim Ersam2*
1
Universitas Pattimura–Indonesia
2
Institut Teknologi Sepuluh Nopember –Indonesia
E mail: healt_kainama@yahoo.com
Oral Presenter
ABSTRACT
Garcinia lasoar is an endemic plant from Maluku-Indonesia. The stem bark of G.lasoar used
empirically in traditional medicine as local wisdom. However antibacterial activities has never been
reported. This study aim is to determine the antibacterial activity of petroleum ether extract (PEE)
and n-hexane extract (HX) of stem bark G.lasoar with broth dilution method in concentration 500 to
31.12 μg/mL. The antibacterial activities of petroleum ether extract was also evaluated for Gram-
positive and Gram-negative bacteria with chloramphenicol and ampicilin as positive control. The
PEE and HX showed that antibacterial activities against Staphylococcus aureus with IC50 values of
5.92 ± 0.04 µg/mL and 59.73 ± 0,04 µg/mL, respectively. The antibacterial activities of both extracts
was lower than positive controls of chloramphenicol ((IC50 value of 0.0007 ± 0.01 µg/mL) and
ampicilin (IC50 value of 1.49 ± 0.03 µg/mL). The PEE and HX in concentration 500 μg/mL can
inhibit of Eschericia coli by only 53.39 ±0.03%. The inhibition of S.aureus in this concentration
showed similar to positive controls. This matter can be predicted as a synergism of terpenoids,
alkaloids, phenolics, flavonoids and tannins in extracs. In addition, the chemical composition of PEE
(five compounds) and (seven compounds) have effect to each of antibacterial activity. Thus PEE can
be used as an antibacterial source.
Keywords: antibacterial, endemic plant, Garcinia lasoar, Staphylococcus aureus, Maluku

141
Effect of Drying Temperature on Characteristics of Gelatin from Nila Fish
(Oreochromis niloticus) Skin
Suryanti, Theresia Dwi Suryaningrum and Bagus Sediadi Bandol Utomo
Research Centre and Development for Marine and Fisheries Product Processing and Biotechnology,
Jakarta, Indonesia.
suryanti_jkt@yahoo.com or yantijkt1975@gmail.com / Suryanti
Oral Presenter
ABSTRACT
This study aims to determine the effect of drying temperature of gelatin extract from nila fish
(Oreochromis niloticus) skin on the quality characteristics. Production of gelatin by using 0.05M
acetic acid with processing step was degreassing, demineralization, washing, extraction and drying.
Degreassing was by immersing skin in water at a temperature of ± 60 ° C for ± 10 seconds, then
draining it. Demineralization was carried out by immersing skin in a solution of 0.05 M for 2 hours
and washing process until reached neutral pH, and extracted with distilled water (1: 3 / w: w) at 80
o
C for 3 hours. Filtration was using a filter cloth and drying at room temperature (20 - 25 oC) and
oven on 55 oC until dry. Observations on gelatin for yield, proximat composition, gel strength, color,
and IR spectra of the gelatin molecule groups. Gelatin obtained from drying and oven on 55 oC has
a lower moisture content than drying temperature (20-25oC) but has higher protein content, and for
ash and fat content was not significant different. Gelatin obtained from drying oven 55 oC has yield
of 17% and gel strength of 226 g, drying temperature (20-25oC) has yield of 23% and gel strength
240 g. Color of gelatin measured based on the values of L, a *, b * which gelatin obtained from
drying oven at 55oC was greater than drying room temperature (20-25 oC). IR spectra of the gelatin
molecular groups from the two drying temperatures did not different, which have spectra at between
1336 cm-1 and 1744 cm-1. It means both of these gelatin have double helix structure molecule which
obtained from htdrolysis process of triple helix structure molecule of collagen during extraction
process.
Keyword: gelatin, nila fish, drying, temperature.

142
Synthesis and Characterization of NaY Zeolite From Capkala Kaolin with
Variation Crystallization Time
Muhammad Kafillah, Novia Amalia Sholeha, Didik Prasetyoko
Indonesia
muhammadkafillah91@gmail.com
Oral Presenter
ABSTRACT
Capkala kaolin from Bengkayang, West Borneo could be used as a raw material for NaY zeolite
synthesis because its main mineral content was constituted from 67% SiO 2 and 22% Al2O3 of total
weight. Those high content of silica and alumina on Capkala kaolin could be transformed to
aluminosilicate gel through the formation of sodium silicate and sodium aluminate as the precursor
of NaY zeolite.
This research aimed to determine the effect on synthesis of NaY zeolite from Capkala kaolin using
hydrothermal method. Prior to synthesis of NaY zeolite, Capkala kaolin initially treated by alkaline
fusion method with weight ratio of kaolin/NaOH 1:3. NaY zeolite was synthesized through two
stages of making seed gel with the molar ratio of 10,67 Na2O: 4 SiO2: Al2O3 : 180 H2O and feedstock
gel with the molar ratio of 4,3 Na2O: 10 SiO2: Al2O3 : 180 H2O. The synthesized feedstock gel was
added to the seed gel to form overall gel. The mixture was then transferred to 250 mL polypropylene
bottle for crystallization. The variation of crystallization time used in this research were 6, 12, 18 and
24 hours with hydrothermal temperature at 100ºC. The synthesized zeolite was characterized by FTIR
spectroscopy and X-ray diffraction. The experimental result reported that the mixture of NaY and
NaP zeolite at 6, 12 and 18 hours. Meanwhile, crystallization time at 24 hour showed result that pure
NaP zeolite.
Keywords: alkaline fusion, crystallization time, hydrothermal, Capkala kaolin, NaY zeolite

143
Synthesis of Zeolite A Supported Fiber and Its Aplication for CH4 Adsorption
Novia Amalia Sholeha1, Nurul Widiastuti1, Didik Prasetyoko1, Aishah Abdul Jalil2,3
1
Indonesia
2
Departement of Chemical Engineering, Faculty of Chemical and Energy Engineering, Universiti Teknologi
Malaysia, Malaysia
3
Centre of Hydrogen Energy, Institute of Future Energy, Universiti Teknologi Malaysia, Malaysia
noviaamaliasholeha@gmail.com
Oral Presenter
ABSTRACT
Methane is the largest component of natural gas and has widely known as an alternative fuel for fossil
fuels. Although the gravimetric energy density value of natural gas is greater, natural gas has
weaknesses in storage techniques. One of the reported materials that are developed for CH 4
adsorption is zeolite-A (LTA). This research aims to determine the effect of alkali activation
conditions in the synthesis of zeolite-A onto fiber. The molar ratio used in the zeolite-A synthesis
through hydrothermal process was 3,165 Na2O: 1 Al2O3: 1,926 SiO2: 128 H2O. The results of
characterization by using XRD showed that zeolite-A had characteristic of peak at 2Ɵ = 7.18; 10.07;
12.46; 16.11; 21.67; 23.99; 27.11; 29.94; and 34.18°. Moreover, SEM analysis displayed that zeolite-
A covered the surface of those fiber when the concentration of NaOH was 4 M. The methane
adsorption capacity of the catalysts was 4.644 % wt.
Keywords: Zeolite-A, fiber, alkali, methane

144
The Effect of Different Type of Additional Silica Source on The NaY Zeolite
Synthesis from Bangka Belitung
Zulfa Nur Aini, Novia Amalia Sholeha, Didik Prasetyoko
Indonesia
Zulfa0202@gmail.com
Oral Presenter
ABSTRACT
Zeolite NaY from Bangka Belitung kaolin had been synthesized through hydrothermal method using
various silica source i.e colloidal silica(L) and sodium silicate(S). The objective of this study was to
understand the effect of the different. The synthesis of zeolite NaY consist of metakaolinization(M),
acid activation on metakaolin with sulfuric acid (MS), aging for 24 hours at room temperature and
crystallization process for 24 hours at 100 °C. The molar ratio of NaY in this study was 6 Na 2O:
Al2O3: 10 SiO2: 180 H2O. The characterizations of synthesized zeolite NaY were performed by using
X-Ray Diffraction (XRD) and Fourier-transform Infared Spectroscopy (FTIR). Based on XRD
pattern and FTIR of the results, zeolite NaY formed using colloidal silica and zeolite P as a
competitive phase in NaY synthesis formed at the variation sodium silicate that indicated excess
crystallization time. Those results confirmed that the solid product were dependent upon the used
additional silica which influenced the phase of the zeolite material.
Keywords: zeolite NaY, Bangka Belitung kaolin, colloidal silica, sodium silicate

145
Preparation of Molecular Imprinted Polymer (MIP) for Selective Extraction of
Gallic Acid
L. S. Adi, D. A. Nurania), dan D. U. C. Rahayu
a)
Oral Presenter
ABSTRACT
Gallic acid (GA) can be found in a variety of foods, such as vegetables and fruits. Extraction of
GA is influenced by the complex nature of sample matrices, therefore selective separation is
required. The molecular imprinted polymer (MIP) method is cited as one of the most promising
methods because it has specific cavities sites of molecular target. MIP has many advantages, i.e.
can be synthesized at a low cost, has good stability under various physical and chemical
conditions, and has good reusability. In this study, molecular imprinted polymer has been
successfully synthesized by using gallic acid as a template in the porogen ethanol and
copolymerized by methyl methacrylate as a functional monomer and ethylene glycol
dimethacrylate as a cross-linker in the presence of 2,2’-azobisisobutyronitrile as an initiator. The
synthesized polymers were characterized by FTIR, SEM-EDX, and TGA. The isotherm study
reveals that MIP has multi layers binding sites that follows Freundlich equation with R 2 = 0.9625.
MIP shows the high adsorption capacity 3.29 mg/g at pH 5 for 90 minutes as compared to non-
imprinted polymer. The selectivity study showed the distribution coefficient of gallic acid (GA)
and ascorbic acid (AA) adsorption were 1.54 and 0.83, respectively, with relative selectivity
coefficient of GA to AA was 4.52.
Keywords: Molecular imprinted polymer, gallic acid, polymerization, adsorption

146
Analysis of Antioxidant Activity of Black Tea Extract (Camellia sinensis L.) and
the Effect on Organoleptic Properties of Cassava Getuk
Wahyu Dwi Gunawan1, Zjahra Vianita Nugraheni1, a), Hidayah Nur Laili1, Herdayanto Sulistyo
Putro1, Agus Wahyudi1
1
Surabaya, Indonesia
a)
Corresponding author: zjahravianita@chem.its.ac.id
Oral Presenter
ABSTRACT
The research of antioxidant activity of black tea extract and the effect of its addition on
organoleptic properties of cassava getuk has been successfully carried out. Black tea was extracted
using maceration method with water as solvent. Extraction was carried out with variations in the
immersion temperature, namely 50, 60, 70, 80, and 90°C. Testing of phenolic content, flavonoids,
and antioxidant activity was carried out on each black tea extract. The optimum result of phenolic
and flavonoid content were 1,003.346 ± 5.445 μg GAE/g of black tea and 320.065 ± 1.246 μg
QE/g of black tea at 80°C, respectively. The optimum antioxidant activity (% decolorization) was
46.798 % at 60°C. Organoleptic test results showed that panelists preferred cassava getuk without
the addition of black tea extract.
Keywords: Black Tea, Antioxidant Activity, Cassava Getuk.

147
Effect of Addition Black Tea Extract (Camellia Sinensis) on Antioxidant Activity
of Pukis Cake
Zjahra Vianita Nugraheni1,a , Hidayah Nur Laili1, Wahyu Dwi Gunawan1, Herdayanto Sulistyo
Putro2, Agus Wahyudi1, Yulfi Zetra1, R.Y Perry Burhan1
1
Molecular Geochemistry Laboratory, Department of Chemistry, Institut Teknologi Sepuluh Nopember,
Surabaya, Indonesia
2
Microorganism Chemistry Laboratory, Department of Chemistry, Institut Teknologi Sepuluh Nopember,
Surabaya, Indonesia
a)
Corresponding author: zjahravianita@chem.its.ac.id
Oral Presenter
ABSTRACT
Black tea contains a variety of active compounds that are proven to have health effects for the
body, one of which is antioxidant properties. Therefore, black tea can be used as an additive for
making functional foods. In this study extraction of black tea using maceration method with water
solvent has been done. Extraction was carried out with temperature variations of 50, 60, 70, 80,
and 90 °C. Each extract obtained was analyzed to determine the total phenolic content, total
flavonoids, and their antioxidant activity. The optimum results obtained in the test total phenolic
and total flavonoids content were 1003,346 ± 5,445 μg AGE / g of black tea and 320,065 ± 1,246
μg KE / g of black tea at a temperature variation of 80 °C. While the optimum results of the
antioxidant activity of black tea extracts were obtained at 60 °C with a percent decolorization were
46.798%. Addition of black tea extract to pukis cake can increase total flavonoid content,
antioxidant activity, resistance to molds, and affect color and texture in organoleptic tests. The
greatest antioxidant activity was obtained from the addition of black tea extract and coconut milk
(2: 3) with a percent decolorization of 89,269%.
Keywords: Black tea, antioxidant activity, pukis cake, functional food.

148
Biodecolorization and Biotransformation of Methylene Blue by Mixed Cultures
of Brown-rot Fungus Daedalea dickinsii and Filamentous Fungus Aspergillus
oryzae
Umirul Solichah Fauzany1, Adi Setyo Purnomo1, a), Hamdan Dwi Rizqi1, and Taufiq Rinda Alkas1
1
Department of Chemistry, Institut Teknologi Sepuluh Nopember (ITS), Kampus ITS Sukolilo,
Surabaya, 60111, Indonesia
Corresponding author E-mail: adi_setyo@chem.its.ac.id; adi.spurnomo@yahoo.com (A.S.
Purnomo)
Tel / Fax: +62-31-5943353 / +62-31-5928314
Oral Presenter
ABSTRACT
This study investigated the removal and transformation of methylene blue (MB) by mixed cultures
of brown-rot fungus Daedalea dickinsii and filamentous fungus Aspergillus oryzae. The ratio of D.
dickinsii and A. oryzae in mixed cultures was 1:1, which was incubated at 30 °C for 7 days in liquid
medium potato dextrose broth (PDB) The results showed that the cultures had the ability to remove
and transform MB (final concentration of MB was 95.24 mg L-1). Mixed cultures of D. dickinsii and
A. oryzae had the highest MB removal of 64.77%, while the single culture D. dickinsii and A. oryzae
only were 5.94% and 36.82%, respectively. Based on the result of LC-TOF/MS analysis, the peak
intensity of MB compound (m/z 284) on each culture’s chromatogram treatment had decreased
compare with the chromatogram control. The metabolites of MO decolorization by D. dickinsii were
C15H16N3S, C16H19N3SO, and C16H21N3SO, while metabolites of A. oryzae was C31H48N3S+. Besides,
the MO metabolites of mixed cultures were C26H37N2O3S, C9H8N2O3S, C28H38NO2S, and
C27H27N5S2. These results showed that mixed cultures of D. dickinsii and A. oryzae has high
significant of decolorization of MB.
Keywords: Biodecolorization; Biotransformation; Methylene blue; Daedalea dickinsii; Aspergillus

oryzae.

149
PVDF/LiCl Membrane For Up-Concentration Of Nannochloropsis sp.
Microalgae Harvesting And Its Cleaning
Badrut Tamam Ibnu Ali1, Marisa Permatasari1, Nurul Widiastuti1,a, Yuly Kusumawati1, Dini
Ermavitalini2, Taufik Qodar Romadiansyah1, Widi Citra Lestari1
1
2
Department of Biology, Faculty of Science and Data Analytics, Institut Teknologi Sepuluh Nopember,
a)
Oral Presenter
ABSTRACT
Increased concentration of Nannochloropsis sp. microalgae during harvesting process is a great

importance economically. Separation of Nannochloropsis sp. using Polyvinylidene Fluoride
(PVDF) polymeric membrane was studied with addition of 2% LiCl to improve up-concentration
performance. The Hermia model is used to predict the type of fouling on the membrane. Cleaning
with NaOCl 1000 ppm solution was carried out to overcome fouling on the membrane.
PVDF/LiCl membranes were prepared using the immersion-precipitation phase inversion method.
The results showed that the addition of LiCl caused an increase in membrane hydrophilicity as
indicated by a decrease in the value of the contact angle from 94.8° to 70-84°. Membrane
morphology changed from finger-like to sponge-like. Concentration of Nannochloropsis sp.
increased from 563.92 to 5097.5 ppm. The membrane performance after cleaning is still very good
because it produces recovery is more than 89.924%, and the microalgae rejection is 100%. It was
concluded that the PVDF membrane after the addition of LiCl could improve the up-concentration
performance of Nannochloropsis sp. and NaOCl solution was very effective in overcoming
fouling on the membrane.
Keywords: Polyvinylidene Fluoride, Polymeric Membrane, LiCl addition, Up-concentration,

Cleaning.

150
Content and Morphology of Skeletonema Costatum on Nutrient N and Si Stress
Lipid
1
(Tutik Nurhidayati, Department of Biology, Institut Teknologi Sepuluh Nopember, Surabaya, Indonesia, Jl.
Arief Rahman Hakim Kampus ITS Surabaya 60111, Indonesia) btutikn72@gmail.com
2
(Hery Purnobasuki, Departement of Biology, Faculty of Science and Technology, Airlangga University.
Surabaya, East Java 60115. Indonesia, Indonesia).ahery-p@fst.unair.ac.id
1
(Wahyu Osi D. P.,Department of Biology, Institut Teknologi Sepuluh Nopember, Surabaya, Indonesia, Jl.
Arief Rahman Hakim Kampus ITS Surabaya 60111, Indonesia), eosiwahyu@gmail.com
1
(Wirdatul Muslihatin, Department of Biology, Institut Teknologi Sepuluh Nopember, Surabaya, Indonesia,
Jl. Arief Rahman Hakim Kampus ITS Surabaya 60111, Indonesia), ewirdhabioitsn@gmail.com
Oral Presenter
ABSTRACT
Skeletonema costatum is a diatom microalgae that can be used as one candidate for biodiesel because
it has a TAGs 8% lipid content of dry weight with doubling time 0.340 cells/day. This study aims to
determine the effect of N and Si nutrients on the lipid content and morphology of S. costatum. The
method begins with a preliminary test of S. costatum culture with different concentrations of nutrient
N and Si, then measured cell density with Haemocytometer for making growth curves. The
observation parameters in this study included analysis of quantitative lipid content using the soxhlet
method and qualitatively using fluorescence microscopy, and morphological observations of S.
costatum using SEM. The design used was RAL and then analyzed using one way ANOVA (α =
0.05) and continued by the Tukey test. The results showed that nutrient N and Si stress proved the
lipid content and morphology of S. costatum. The less amount of nutrients N and Si in the growth
media, the total lipid content of S. costatum increased, while the management of N3Si2 (10.25%) and
N2Si2 (6.75%) differed significantly from the control (1.75%). The lipid content of TAGs was
approved by the presence of a yellow color on N3Si2 administration. Morphological observations of
S. costatum on the implementation of N3Si have changed the size of the S. costatum cell length,
which is 3.9 µm 70% smaller than the normal size of the control 10.7 µm.
Keywords: Morphology, Skeletonema costatum, Stress Nutrient (N and Si), Total Lipid, and TAGs

151
WonoYo: Improving Local Dairy Product Potential by Combining Fortification
of Yogurt Fermentation and Online Branding Marketing
Herdayanto Sulistyo Putro1 , a , Fahimah Martak1, Mardi Santoso1, Taslim Ersam1, Sri Fatmawati1,
Refdinal Nawfa1, Setiyo Gunawan2, Agus Raikhani 4, Ahnaf 1, Haritsa Syafdhani1, Fery Anugra 1,
Matt Bussar 1, Yusuf Syahril Alam 1, Achmad Rizal Hamzah 1, I’tatina Ayyu Rizqika 1, Frida
Wahyu Hairunnisa 1, Inne Febriyanti 3, Rifda Wiedsah 3, Adiniar Lailatul Melinda 1, Nur Hafidzah
Oktavia 1, Shalita Nafisah Putri Wahyudhie 1, Rachma Anni Ziyana 1, Farah Mahzumi 1, Izzah
Maulidah 1, Berliana Gricelda Andini Putri 1, Hanggi Fragandi 1, Agus Raikhani 4
1
Department of Chemistry, Institut Teknologi Sepuluh Nopember, Sukolilo, Surabaya 60111, Indonesia
2
Department of Chemical Engineering, Institut Teknologi Sepuluh Nopember, Sukolilo, Surabaya 60111,
Indonesia
3
Department of Business Management, Institut Teknologi Sepuluh Nopember, Sukolilo, Surabaya 60111,
Indonesia
4
Faculty of Engineering, Universitas Darul ‘Ulum, Jombang 61419, Indonesia
a)
Corresponding author: hsputro@chem.its.ac.id
Oral Presenter
ABSTRACT
Carangwulung Village has been known for their dairy farmers. They sell their products locally and
traditionally. As the production continues growing, it becomes a necessity to process fresh milk
further into different type of products with better economic value, such as ice cream, cheese, and
yoghurt. Yoghurt is one of the main dairy products which contains many benefits for our body. Thus,
yoghurt product was proposed to the farmers in order to improve the product quality and increase
farmer’s income. In this project, experiment was carried out in the laboratory to find a suitable
method for yogurt composition before being taught to the community. The aims of this project are
creating yoghurt with unique taste and providing halal certification training to supply insight mindset
of making better local products as well as halal labels. The resulting product is also marketed through
social media to increase sales. It is hoped that with this program, the economy of the Carangwulung
Village community will increase.
Keywords: Yoghurt, Community Service, Halal, Branding, Carangwulung

152
Biotransformation of Methylene Blue by Mixed Fungal Cultures of
Gloeophyllum trabeum and Aspergillus oryzae
Adelia Sabilah Prameswari1, Adi Setyo Purnomo1, a), Hamdan Dwi Rizqi1, and Taufiq Rinda Alkas1
1
(ITS), Kampus ITS Sukolilo, Surabaya, 60111, Indonesia.
Corresponding author E-mail: adi_setyo@chem.its.ac.id; adi.spurnomo@yahoo.com (A.S. Purnomo)
Tel / Fax: +62-31-5943353 / +62-31-5928314
Oral Presenter
ABSTRACT
This study investigated the biodecolorization of Methylene Blue (MB) by a mixed fungal cultures
of Gloeophyllum trabeum and Aspergillus oryzae. A. oryzae (10 mL) was added into G. trabeum
cultures (10 mL) and then MB was added until the final concentration reached 95,24 mg/L and
incubated at 30 °C for 7 days. All of mixed and single cultures had the ability to decolorize MB on
the liquid media of potato dextrose broth (PDB). The addition of A. oryzae to G. trabeum culture
showed the highest MB decolorization of 83.18%, whereas by G. trabeum alone it was only 65.72
% and A. oryzae alone 72.84 %. C16H20N3S, C19H22N3SO4, and C31H48N3S+ were identified as
product metabolites of MB decolorization by the mixed culture. This study indicated that the
addition of A. oryzae enhanced decolorization of MB by G. trabeum.
Keywords: Biodecolorization, Methylene blue, Mixed culture, Gloeophyllum trabeum, Aspergillus
oryzae.

153
Biodecolorization and Biotransformation of Methylene Blue by Mixed Cultures
of Brown-rot Fungus Gloeophyllum trabeum and Filamentous Fungus
Trichoderma viride
Ninda Intan Pratiwi1, and Adi Setyo Purnomo1, a), Hamdan Dwi Rizqi1, Taufiq Rinda Alkas1,
Refdinal Nawfa1
1
(ITS), Kampus ITS Sukolilo, Surabaya, 60111, Indonesia.
Corresponding author E-mail: adi_setyo@chem.its.ac.id ; adi.spurnomo@yahoo.com (A.S. Purnomo)
Tel / Fax: +62-31-5943353 / +62-31-5928314
Oral Presenter
ABSTRACT
In this study, mixed cultures of brown-rot fungus Gloeophyllum trabeum and filamentous fungus
Trichoderma viride in decolorizing Methylene Blue (MB) has been investigated. T. viride culture
was mixed with G. trabeum culture then MB was added into the media to achieve final concentration
95.24 mg/L. The results showed single culture of G. trabeum and T. viride can decolorize MB only
31.50% and 53.89%, respectively. While, mixed cultures G. trabeum and T. viride reached to 74.72%
in a liquid medium of potato dextrose broth (PDB) for 7 days. The results showed mixed cultures had
the highest ability to decolorize MB among those variations. Based on the LC-TOF MS analysis, the
peak of MB in mixed cultures treatment has a lower intensity compared to the peak of MB in abiotic
control. The prediction of the product metabolites from biodecolorization G. trabeum were
C8H10N2SO, C6H8N2SO3, C16H20N3SO, C6H7NSO3, and C12H10N3S, while the metabolites from
biodecolorization T. viride were C16H23N3O3S, C22H26NO2S, and C31H48N3S. On the otherhand, the
metabolites from the MB biodecolorization by mixed cultures were C 16H23N3O3S, C31H48N3S,
C31H47N2OS and C17H20N2S. This study indicated that mixed cultures of brown-rot fungus G.
trabeum and filamentous fungus T. viride effective to decolorize MB dye.
Keywords: Biodecolorization; Methylene Blue; Gloeophyllum trabeum; Trichoderma viride.

.

154
The Antibacterial activity of Vanillin Derivative Compounds
Rini Retnosari1*, Ihsan Budi Rachman1, Sutrisno1, Meyga Evi Ferama Sari1, Dedek
Sukarianingsih1, and Yaya Rukayadi2
1
Department of Chemistry, Faculty of Mathematics and Natural Science, Universitas Negeri Malang, Jl.
Semarang No. 5 Malang, Indonesia
2
Faculty of Food Science and Technology and Institute of Bioscience, Universiti Putra Malaysia, 43400
UPM Serdang, Selangor Darul Ehsan, Malaysia
*Corresponding author: rini.retnosari.fmipa@um.ac.id
Oral Presenter
ABSTRACT
The aim this study was to perform synthetic compound of 9-(4-hydroxy-3-methoxyphenyl)-3,3,6,6-

tetramethyl-3,4,5,6,7,9-hexahydro-1H-xanthene-1,8(2H)-dione (1a) and 9-(3-ethoxy-4-
hydroxyphenyl)-3,3,6,6-tetramethyl-3,4,5,6,7,9-hexahydro-1H-xanthene-1,8(2H)-dione (1b) (1,8-
dioxo-hydroxanthene derivates) derivate from vanillin and to determine their antibacterial activity
against various bacteria such as Staphylococcus epidermidis, Propionibacterium acnes,
Staphylococcus epidermidis, Bacillus subtilis, Salmonella Typhimurium, and Pseudomonas
aeruginosa. Compound 1a and 1b were synthesized from dimedone and aromatic aldehyde (vanillin
and ethyl vanillin) in the presence of concentrated sulfuric acid and ethanol as solvent. Their
antibacterial activity was determined by well diffusion assay method with measuring zone of
inhibition in mm. Two derivatives of 1,8-dioxo-octahydroxanthene (compound 1a and 1b) has been
successfully synthesized. Compound 1b has strong antibacterial activity against Staphylococcus
epidermidis and compound 1a has medium antibacterial activity against Propionibacterium acnes,
Staphylococcus epidermidis, Bacillus subtilis, Salmonella Typhimurium, and Pseudomonas
aeruginosa. The replacing methoxy group with ethoxy group attached on aromatic aldehydes
enhance the antibacterial activity of 1,8-dioxo-hydroxanthene compounds.
Keywords: vanillin, ethyl vanillin, 1,8-dioxo-octahydroxanthene, antibacterial agents

155
Catalytic Cracking Polystyrene Waste by Co-Reactant Biodiesel Waste Coconut
Oil and Its Performance at Gasoline Engine
H. Juwono1, A.Hawky1, W. Trisunaryanti2, P. S. Utama2, L. Efiyanti3, S. l. Bahri4, K. Amri5, A.
Assari1 and Y. L. Ni’mah1
1
Chemistry Department, , Institut Teknologit Sepuluh Nopember, Surabaya, Indonesia
2
Chemistry Department, Universitas Gajah Mada, Indonesia
3
Pusat Penelitian Dan Pengembangan Hasil Hutan, BaLitbang Dan Inovasi, KLHK, Indonesia
4
Chemical Engineering Department, Universitas Riau Kampus Binawidya, Indonesia
5
Balai Teknologi Bahan Bakar dan Rekayasa Desain Badan Pengkajian dan Penerapan Teknologi ,
Indonesia
yatimnikmah@gmail.com / +6285732108646
Oral Presenter
ABSTRACT
Liquid fuel from polystyrene plastic waste (PS) with used cooking oil biodiesel co-reactant was
successfully made. This research was conducted using the catalytic cracking method using Pd/Al-
MCM-41/ceramic catalyst to produce various types of hydrocarbon fractions. The structure,
morphology, chemical bonds and porosity of the catalyst were examined by XRD, SEM-EDX, SEM,
Adsorption-desorption N2, FTIR-Pyridine. Catalytic cracking products were analyzed using Mass
Gas Spectroscopy (GC-MS). The results of GC-MS analysis of liquid fuels from catalytic cracking
showed that the largest content of the gasoline hydrocarbon fraction (C 7-C12). The oil from
PS/Kat120 catalytic cracking has the largest gasoline fraction of 85.64%. More gasoline fractions
were formed at the time of catalytic cracking for 120 minutes compared to 60 minutes. The liquid
fuel produced by catalytic cracking is added by Premium RON 88 and MTBE additives for research
on fuel performance using gasoline engines. Physical characteristics in the form of density, flash
point, calorific value, and octane number of a mixture of liquid fuels were also examined. The mixture
of CPSJ/Kat2 liquid fuel has the highest percentage of thermal efficiency of 26.33% with a density
value of 0.7515 g/ml, a flash point of -48.7℃, a heating value of 19160.61 kcal k, and an octane
number of 95.6. Characteristics of a mixture of liquid fuels produced in accordance with SNI 06-
3506-1994. In addition, the characteristics of the liquid fuel produced are also in accordance with the
standard of commercial gasoline.
Keywords: Polystyrene waste, liquid fuels, catalytic conversion, reusability number

156
The Ability of Mixed Cultures Daedalea dickinsii and Trichoderma viride on
Biodecolorization of Methylene Blue Dye
Santika Damayani1, Adi Setyo Purnomo1,a, Hamdan Dwi Rizqi1, Taufiq Rinda Alkas1, and
Herdayanto Sulistyo Putro1
1Department of Chemistry, Faculty of Science and Data Analytics, Institut Teknologi
Sepuluh Nopember (ITS), Kampus ITS Sukolilo, Surabaya 60111, Indonesia.
a)
Corresponding author
E-mail: adi_setyo@chem.its.ac.id ; adi.spurnomo@yahoo.com (A.S. Purnomo)
Tel / Fax: +62-31-5943353 / +62-31-5928314
Oral Presenter
ABSTRACT
This research aims to study biodecolorization of Methylene Blue (MB) by mixed culture of Daedalea
dickinsii and Trichoderma viride. Ten milliliters of filamentous fungus Trichoderma viride was
added into 10 mL of brown-rot fungus D. dickinsii culture. The results showed mixed cultures had
ability to decolorize MB in potato dextrose broth (PDB) after 7 days of incubation. The mixed
cultures decolorized MB up to 65.07%, whereas a single culture of Daedalea dickinsii and
Trichoderma viride could only decolorized MB up to 5.94% and 53.92%, respectively. The report of
LC-TOF/MS analysis found that there were some metabolites peaks due to the formation of
C17H20N2S and C31H47N2OS. This research showed that the mixed fungal cultures of Daedalea
dickinsii and Trichoderma viride can improve the decolorization of MB dye.
Keywords: Biodecolorization, Methylene Blue, Daedalea dickinsii, Trichoderma viride

157
A New Route Synthesis of Al-MCM-41 From Red Mud Waste for Reutealis
Trisperma Oil Deoxygenation
Muhammad Navis Muhaimin, Didik Prasetyoko, Reva Edra Nugraha, Holilah
Institut Teknologi Sepuluh Nopember (ITS), Surabaya, Indonesia
Navis16@mhs.chem.its.ac.id
Oral Presenter
ABSTRACT
Originated from the by-product of bauxite processing in alumina production, red mud solid waste
has caused severe environmental pollution. In this study, we reported the synthesis of Al-MCM-41
from red mud waste modified with citric acid treatment as catalyst for Reutalis trisperma oil
deoxygenation. Al-MCM-41 was synthesized at 80 ℃ for 12 h and 150 ℃ for 24 h. Citric acid
treatment has significantly reduce the Iron (Fe) content in red mud waste. Furthermore, the XRD
analysis showed the reducing content of Quartz phase and the formation of amorphous Al-MCM-41.
TGA analysis indicated the weight loss of water moisture and CTAB surfactant which confirm the
presence of mesoporous phase in Al-MCM-41. The deoxygenation reaction was conducted at 350 ℃
for 4 h under nitrogen atmosphere. The GC-MS analysis showed that the deoxygenated liquid product
consist of hydrocarbon, aromatic, carboxylic acid and cyclic compounds. Moreover, Al-MCM-41
has high selectivity towards diesel range (C11-18) hydrocarbon up to 90%.
Keywords: Al-MCM-41, deoxygenation, red mud, hydrocarbon, diesel

158
The Effect of Various Templates for Synthesis Aluminosilicate as Catalysts on
Oleic Acid Deeoxygenation Reaction
Hari Purnomo, Didik Prasetyoko, Reva Edra Nugraha
Institut Teknologi Sepuluh Nopember , Indonesia
Hrpurnomo17@gmail.com
Oral Presenter
ABSTRACT
Oleic acid is a mono unsaturated fatty acid which mainly exist in vegetable oil that can be used as a
model compound for deoxygenation reaction to produce fuels. There are several method used to
produce fuels from oleic acid such as: cracking, trans/esterification and deoxygenation. In this study,
green diesel were obtained through the process of oleic acid deoxygenation with various templates
in the synthesis of aluminosilicate catalysts, i.e (Ni/ZSM-5(Seed), Ni/ZSM-5 (TPAOH) and Ni/Al-
MCM-41(free-template)). The total weight of the metal loading is 10% wt. The catalysts were
synthesized under hydrothermal method at 80℃ and 150 ℃ with molar ratio of Si/Al=25. The
obtained catalysts were characterized using X-ray diffraction (XRD), Fourier transform infra-red
(FTIR) spectroscopy and nitrogen adsorption-desorption isotherm analysis. The catalytic test were
performed in semi-batch reactor at 320oC under N2 flow (50 ml/min) . Furthermore, the obtained
product from catalytic test were characterized by gas chromatography – mass spectrometry. The
analysis result shows that Al-MCM-41 (free-template) catalyst gives the highest yield hydrocarbon
of 88.56% followed by ZSM-5 (seed) and ZSM-5 (TPAOH), 84.43% and 80.90%, respectively.
However, the selectivity of diesel range hydrocarbon (C13-C17) ZSM-5 with TPAOH template exhibit
the highest value of 65.61% followed by ZSM-5 (Seed) and Al-MCM-41 (free-template), 62.89%
and 31.06 %, respectively.
Keywords: Oleic acid, fuels, green diesel, templates, deoxygenation.

159
The Effect of Variation Linker’s Amount on Synthesis of Mesoporus ZnO for
Photodegradation of Methylene Blue
Abdul Aziz1, Didik Prasetyoko1*, Maria Ulfa2, Hasliza Bahruji3, Mokhammad Fajar Pradipta4
1
Department of Chemistry, Faculty of Science and Data Analytics, Institute of Technology Sepuluh
Nopember, Jl. Keputih, Surabaya, East Java Indonesia
2
Study Program of Chemistry Education, Faculty of Teacher Training and Education, the March University,
Jl. Ir. Sutami 36A, 57126 Surakarta, Central Java Indonesia
32
Centre of Advance Material and Energy Science, Brunei Darussalam University
4
Department of Chemistry, Faculty of Mathematics and Natural Sciences, Gadjah Mada University, Jl.
Sekip Jogja, Central Of Java, Indonesia
*didikp@chem.its.ac.id
Oral Presenter
ABSTRACT
Mesoporous ZnO was attractive to use for photodegradation because of its high surface area and
unique geometric shape. ZnO was synthesized using gelatin as natural colloidal system instead of
synthetic which were expensive and difficult to be decomposed. In this study, the variation
concentration of gelation as linker concentrations (0.01, 0.03, 0.06) in a reflux reactor was carried
out as a control for ZnO porosity for the photodegradation of methylene blue. Properties of
mesoporous ZnO were characterized by XRD, FTIR, and SEM-EDX. The main peaks in the
diffraction pattern were observed at 2θ values of about 7.88, 16.08, 21.11, 24.35, 27.52, 32.98, 33.56,
34.94, 35.44, and 38.68°. The catalytic activity showed only 10.5% removal for ZnO with the
concentration of 0.06.
Keywords: ZnO, gelatin, photodegradation, methylene blue

160
The Adsorption and Photocatalytic Activity Test of ZSM-5 for Methylene Blue
Decolourization
Riki Subagyo1, Novia Amalia Sholeha1, Reva Edra Nugraha1, Holilah1, Didik Prasetyoko1*
1
Department of Chemistry, Faculty of Science and Data Analytics, Institute of Technology Sepuluh
Nopember, Jl. Keputih, Surabaya, East Java Indonesia
*didikp@chem.its.ac.id
Oral Presenter
ABSTRACT
ZSM-5 from red mud as bauxite waste was prepared by hydrothermal method at molar composition
of 10Na2O: 100SiO2: 2Al2O3: 1800H2O. ZSM-5 was characterized by XRD dan FTIR. The
characterization result show that ZSM-5 have MFI structure, that can be seen on the XRD results at
at 7.9°; 8.8°; 23.1°; 23.9° and 24.3° as a characteristic peak of ZSM-5 MFI. The absorption peaks at
540 (in the FTIR results) attributed to the as asymmetric stretching vibration of double five-
membered ring (D5R) which is characteristic of pentasyl MFI-type zeolit structure. The adsorption
and photocatalytic activity test of ZSM-5 for methylene blue decolourization were carried out in dark
condition and under UV-LED irradiation, respectively. The result of adsorption and photocatalytic
activity test show that ZSM-5 only undergoes adsorption processes either in dark conditions or in
UV-LED irradiation.
Keywords: Adsorption, Photocatalytic, ZSM-5, Decolourization, Metehylene Blue

161
Bio-degradation of Methylene Blue (MB) by Ralstonia pickettii
Asranudin1, Adi Setyo Purnomo1*, Didik Prasetyoko1, Yulinar1
(ITS), Kampus ITS Sukolilo, Surabaya 60111, Indonesia
*adi_setyo@chem.its.ac.id ; adi.spurnomo@yahoo.com
Oral Presenter
ABSTRACT
Bio-degradation of methylene blue (MB) dye using the bacterium Ralstonia pickettii was
investigated. MB was degraded in nutrient broth (NB) medium after adding MB at a concentration
of 100 mgL-1. MB degradation used Ralstonia pickettii approximately 98.11% after 18 hours of
incubation. Azure A (AA), Thionin, Leuco-Methylene blue, and C22H31N3SO5 (Glucose-MB) were
detected as MB metabolite products. Based on these metabolite, it was discovered that MB
degradation occurred through a reductase attack on the heterocyclic central chromophore group of
its structure which produced Leuco-MB and Glucose-MB. Meanwhile, the attack on the auxochrome
group by the N-demethylase enzyme resulted in Azure A and Thionin fragments. This is the first
scientific report to utilize Ralstonia pickettii on bio-decolorization and biotransformation of MB.
Therefore, the results of this research indicate that Ralstonia pickettii can be potentially used for the
biodegradation of dye waste
Keywords: Biodecolorization, biotransformation, Ralstonia pickettii, bacteria, methylene blue

162
Synthesis of ZIF-67 in The Presence of Acetic Acid as Methyl Orange Adsorbent
Dety Oktavia Sulistiono and Ratna Ediati
ratna.ediati@gmail.com
Oral Presenter
ABSTRACT
Zeolitic Imidazolate Framework–67 (ZIF-67) materials have successfully been synthesized in

aqueous solution at ratios of Co2+, 2-methylimidazole and triethylamine as deprotonating agent, i.e.
1 : 5 : 5, 1 : 10 : 5 and 1 : 10 : 10 , in the presence and the absence of acetic acid. The XRD patterns
of all synthesized materials showed characteristic peaks at 2 theta values similar to that of simulated
ZIF-67. The results of FTIR analysis showed appearance absorption bands at the same wavenumber
for all synthesized materials. The presence of acetic acid, however, resulted in the decrease in
intensities of the XRD peaks, and altered the surface morphology to become irregular. All materials
were examined their performance as adsorbent for removal of methyl orange in water. It was found
that the highest adsorption capacity of 214 mg/g was shown by ZIF-67(1:10:5 AA). The kinetics and
isotherms adsorption of all synthesized materials followed the pseudo second order kinetics and
Langmuir isotherms.
Keywords: ZIF-67, Adsorption, Methyl Orange, Acetic Acid

163
Synthesis of Al-HKUST-1 and Its Application as A Congo Red Adsorbent
Ratna Ediati a,*, Muhammad Rizal Al Hafiz a, Dety Oktavia Sulistionoca, Yuly Kusumawatia,
Fahimah Martak a
a
ratna.ediati@gmail.com
Oral Presenter
ABSTRACT
HKUST-1 and Al-HKUST-1 with mass addition variation of Al2O3 against HKUST-1 mass of 10,
20, 40, and 80% have been successfully synthesized using the solvothermal method. The
diffractogram patterns of synthesized HKUST-1 and Al-HKUST-1 displayed characteristic peaks at
2θ of 6.5, 9.3, 11.4 dan 13.3°, where all of this match with the standard HKUST-1 diffractogram
pattern. The FTIR spectra of the synthesized solids demonstrated an adsorption band at the same
wavenumber as the reported HKUST-1. The surface morphology of the synthesized HKUST-1 has
an octahedral form, however in the contrary, the synthesized Al-HKUST-1 has a random surface
morphology. The N2 adsorption-desorption data exhibited that the specific surface area of Al(10)-
HKUST-1 and Al(40)-HKUST-1 respectively are 512,6 dan 380,9 m2/g, all of which are smaller than
HKUST-1, which is 784,1 m2/g. The measurement results of the adsorption capacity of Al-HKUST-
1 against congo red dye (CR) in water shows that Al(20)-HKUST-1 has the highest adsorption
capacity of 315,15 mg/g, which follows the pseudo second order kinetic adsorption and Langmuir
isothermal adsorption.
Keywords: HKUST-1, Al2O3, Adsorption, Congo red, Adsorption Capacity

164
Synthesis of Indolin-2-One Derivatives and Their In Vitro Anticancer Activity
Against Widr Cell Line
Arif Fadlan (U), Budi Prayitno, Muhammad Riza Ghulam Fahmi, Mardi Santoso
tsv09@yahoo.com
Oral Presenter
ABSTRACT
The synthesis of indolin-2-one derivatives and in vitro anticancer activity on colon cancer were
reported in the present study. The compounds contain a fluorine or a bromine in position 5, and a
bromine in position 7. The results established that indolin-2-one with bromine in position 5 gave up
to a 4-fold improved cytotoxicity over indolin-2-one with fluorine in position 5. The indolin-2-one
with bromine in posititon 5 also exerted higher cytotoxicity compared to bromine in position 7. This
work highlights the importance of halogen and their position in cytotoxicity.
Keywords: Indolin-2-one derivatives, Anticancer, Synthesis, WiDr

165
Isolated Compound from Stachytarpheta jamaicensis Leaves
Maria Elfiana Ina Kewa Helan, Fadhilatur Rohmah, Sri Fatmawati
fatma@chem.its.ac.id
Oral Presenter
ABSTRACT
Stachytarpheta jamaicensis is one of the plants from Verbenaceae family that has the biodiversity in
Indonesia. This plant has been used as traditional medicine for treating illnesses, such as cough, colds,
fever, constipation, menstrual disorders, respiratory problems, and diabetes. S. jamaicensis contains
secondary metabollites of flavonoids, terpenoids, phenols, alkaloids, glycosides, tannins, and
saponins. The purpose of the research is to reveal secondary metabolites from the ethyl acetate
fraction. The method of S. jamaicensis isolation were fractionated by vacuum column
chromatography with gradient elution, and purified by recrystallization. The isolated compounds
were identified by 1H-NMR, 13C-NMR, HSQC, and HMBC. Two compounds that have been isolated
from leaves of S. jamaicensis. For further research, antioxidant and antidiabetic assays of isolated
compounds are needed to evaluate.
Keywords: Stachytarpheta jamaicensis, isolation, secondary metabolite

166
Antioxidant Activity of Rhodomyrtus tomentosa (W. Ait) Hassk and Melastoma
malabatchricum L. Leaf Extract
Muddatstsir Idris, Adi Setyo Purnomo, Fahimah Martak, Sri Fatmawati
Oral Presenter
ABSTRACT
Rhodomyrtus tomentosa (W. Ait) Hassk and Melastoma malabathricum L. are medicinal plants. Both
of these plants grow a lot in South Kalimantan, Indonesia. The local names of these plants are
karamunting bini (Rhodomyrtus tomentosa (W. Ait) Hassk) and karamunting laki (Melastoma
malabathricum L.). Traditionally, people use both plants to treat various diseases including diabetes.
This study used methanol extracts of R. tomentosa and M. malabathricum leaves to determine
antioxidant and anti-α-glucosidase activity in vitro. The antioxidant test used the DPPH method. The
methanol extract of R. tomentosa and M. malabathricum leaves had high antioxidant activity with
IC50 value of 7.79 and 8.58 μg/mL, respectively.
Keywords: Biological activities, anti-oxidant, secondary metabolites, Rhodomyrtus tomentosa,

Melastoma malabathricum

167
Optimization of Bioactive Compounds of Capsicum annuum
Devi Anggraini Putri, Nur Hasanah, Sri Fatmawati
Oral Presenter
ABSTRACT
Capsicum annuum is a herbaceous plant from Central and South America. This type of capsicum
plant produces many derivatives of capsaicinoids compounds. Capsaicin is the main capsaicinoids
compound in Capsicum annuum which produces a sensation of heat in this plant. This hot sensation
is widely used as a painkiller or analgesic drug. The purpose of this study was conducted to analyze
capsaicin compound in the ethanol extract of Capsicum annuum with various cultivars (big red
chilies, big green chilies, small red chilies and small green chilies). This plant extraction was carried
out by maceration method. After that, the ethanol extract of Capsicum annuum was analyzed for the
content of capsaicin compound by HPLC (High Performance Liquid Chromatography). From the
HPLC chromatogram, it was known that all of the ethanol extracts of Capsicum annuum contained
capsaicin as evidenced by the peak with retention time of 6.040-7.368 minutes. Not only that, the
seeds from red and green chilies contain the highest capsaicin compounds compared to other
variations. The results of this study are expected to become supporting data for further research
(optimization of the extraction method and analgesic test).
Keywords: Capsicum annuum, Capsaicin, HPLC

168
Terpenoid from Chromolaena odorata Leaves
Indah Ayu Risnah, Sri Fatmawati
Oral Presenter
ABSTRACT
Chromolaena odorata leaves, known as “Kirinyu” in Indonesia, is traditionally used as a folk

medicine in several region of the world such as Indonesia, Malaysia, Vietnam, Myanmar, and Nigeria
to cure some illnesses, like burns and soft tissue wounds, hemostatic, hypertension, skin wounds,
cough, and diabetes. Early studies have shown the activity of C. odorata leaves as an antioxidant,
antidiabetic, antibacterial, anti-inflammatory, and anticancer agent. This study aims to determine the
structure of the secondary metabolite that have been isolated. Dichloromethane fraction of C. odorata
leaves was loaded on a silica gel column chromatography with eluent n-hexane: dichloromethane
(increasing polarity) to yield 10 fractions (B1-B10). Fraction B2 was subjected to thin layer
chromatography to afford pure compound (130 mg) as yellow oily liquid. The structure of isolated
compound was identified by 1H-NMR, 13C-NMR, HMBC and HMQC, and indicated as a terpenoid.
For further research, the antidiabetic assay of the isolated compound is recommended.
Keywords: Chromolaena odorata, folk medicine, terpenoid.

169
POSTER PRESENTER

170
Copper (Cu) Content in Sea Water and Sediment and Its Bioavailability in
Balangan Beach Bali
I G. A. Kunti Sri Panca Dewi, Ni G. A. M. Dwi Adhi Suastuti, Marieta Krisensia
Departement of Chemistry Udayana University Bukit Jimbaran
Jurusan Kimia FMIPA Universitas Udayana, Indonesia
Corresponding author: kuntipancadewi@unud.ac.id
Poster Presenter
ID paper : P20200002
ABSTRACT
Balangan Beach is a very popular tourist destination which is located in the south of Bali near
Uluwatu Temple. Because there is a lot of tourist activities which cause pollution. The aimed of study
to determine the concentration of total Cu metals in water and sediments as well as to determine
bioavailability of Cu in the sediments. In order to determine the bioavailability of Cu in the
sediments, the sediment was extracted by the sequential extraction method, then the filtrate was
analyzed by using the Atomic Absorption Spectrophotometry (AAS) brand Shimadzu AA-7000 with
a calibration curve technique. The metal content of Cu obtained in water and sediments was (0.0247-
0.048) mg/L and (3.2742-6.1334) mg/kg respectively. The results of Cu fractionation in sediments
were as follows: EFLE fraction (easily, freely, leachable and exchangeable) of 0.130 mg/kg – 0.787
mg/kg, Fe/Mn oxide fraction of 0.165 mg/kg – 0.819 mg/kg; organic/sulfide of 0.456 mg/kg – 1.641
mg/kg and the resistance fraction of 1.008 mg/kg – 3.478 mg/kg. Therefore, the bioavailable fraction
in the sediment for Cu was 22.92% - 52.93%, and the resistance fraction was 47.06% - 77.06%, this
means that there is little Cu available for organism compared to resistant.
Keywords: Balangan Beach, bioavailability, Cu, sediments, sea water

171
Novel Fast Firing Method to Synthesiza Tetracalcium Phosphate
Muhammad S. Nizar, Naili S., Ria J. M.,
Rizki B.W., Irna R., Dadan S.,
Center for Ceramics, Ministry of Industry Republic of Indonesia
Corresponding author: nizar@kemenperin.go.id
Poster Presenter
ABSTRACT
Tetracalcium phosphate knows as a derivative of apatite base materials that are used in medical and
pharmacy applications such as for bone and dental cement and also for drugs. The synthesis of
tetracalcium phosphate requires several hours of calcining on high temperature furnace. In this paper,
the novel fast firing method was introduced to the synthesis process of tetracalcium phosphate to
modify the solid-state reaction by Ishikawa process to reduce energy and time for producing
tetracalcium materials. One base precursor for tetracalcium phosphate was prepared by reacting
calcium hydroxide and phosphoric acid with molar ratio Ca/P 2:1. The base precursors treated with
different calcination temperatures, time variation, and different quenching methods to verify which
treatment will have high yield tetracalcium phosphate material. The calcination temperature range is
1200oC-1600oC, while the calcination time varied from 3 hours to under 1 hour, also quenched by
air and water. The samples were characterized by XRD to show different mineralogy yield and semi
quantity measurement of tetracalcium content. Synthesized tetracalcium phosphate characterized
with XRD content several apatite minerals which are montetite, brushite, tricalcium, and
hilgenstokite with different ratio percentage content. The semi-quantitative method form XRD
analysis used to calculate the ratio. Tetracalcium phosphate yield range from 7,62%-56,7%. The best
result yield of 56,7% was treated with calcination temperature 1400oC under 1 hour and quenched
with air. The results show that the fast-firing method can optimize the current solid-state reaction
synthesis process, and it looks promising to develop this method to the production process.
Keywords: TetraCalcium Phosphate, biomaterial, fast-firing

172
4-Carboxy Phenyl Diazonium Modified Carbon Paste Electrode (CPE) for
Cu(II) Analysis
Dita N. Arifa, Pratiwi Yuliandari, Rahmat Wibowo
Depok 16424
Corresponding author: ypratiwi168@gmail.com
Poster Presenter
ABSTRACT
Industrial development can increase heavy metal pollution in water. Cu metal is an essential heavy
metal that is needed in a certain amount by organisms, while in excess the amount is capable of
causing toxic properties and environmental pollution. The presence of Cu metal in nature is
considered as a pollutant in the concentration range of 0.001 ppm to 0.015 ppm. The electrochemical
method can be used for detection of heavy metal. In this work, CPE was modified by electroreduction
of 4-carboxypheny diazonium by cyclic voltammetry method by contaminating CPE into a 4-CPD
solution at potential range -1.5 V to 1.0 V with 100 mV?s scan rate for 3 cycles. The modified of
CPE with 4-CPD were characterized using FTIR and SEM-EDX instruments. Analysis of Cu metal
using 4-CPD/CPE electrodes showed a good linearity value of 0.9909 with a low detection limit of
0.1635 ppm with the high sensitivity of 21.506 mA ppm cm -2. From these results it can be seen that
4-CPD/CPE can be used as a good Cu metal detector.
Keywords: 4-carboxyphenyl diazonium, para-amino benzoate, carbon paste electrode, grafting,

electrochemical method

173
Sonication-Assisted Green Synthesis of Silver Nanoparticles Using Aqueous
Extract of Piper retrofractum Fruits and Their Antimicrobial Activity
Suci Amaliyah1, a) Masruri Masruri1, b) Akhmad Sabarudin1,2 c) Sutiman Bambang Sumitro 3,
d)1
Department of Chemistry, Faculty of Science, Brawijaya University, Malang 65145, Indonesia. 2Resesarch
Center for Advanced System and Material Technology, Brawijaya University, Malang 65145,
Indonesia3Departement of Biology, Faculty of Science, Brawijaya University, Malang 65145,
Indonesia.Corresponding author: a)suciamaliyah91.sa@gmail.com b)masruri@ub.ac.id
c)
corresponding author email: sabarjpn@ub.ac.id
d)
sutiman@ub.ac.id
Poster Presenter
ABSTRACT
X`In this study, silver nanoparticle was synthesized using aqueous extract of Piper
retrofractum fruits by the sonication-assisted an eco-friendly method. This extract contains some
compounds that can act as reducing and capping agents for the formation of silver nanoparticles.
Silver nitrate was used as a starting material and was reacted with Piper retrofractum extracts. The
formation of silver nanoparticles was monitored by a color change from colorless to dark brown. The
synthesized AgNPs were characterized using UV-Visible, FTIR, XRD, and FESEM-EDS analysis.
The UV-Vis analysis showed the surface plasmon resonance of silver nanoparticles peak at 476 nm.
FTIR spectrum of silver nanoparticles peaks at 3420 cm -1, 2922 cm-1, 1632 cm-1, and 1021 cm-1 was
similar to the Piper retrofractum extract spectrum, which indicated the compounds might involve in
stabilizing nanoparticles, whereas the additional new peaks at 702 cm -1 and 535 cm-1 revealed the
characteristic band of Ag-O-H. The X-ray diffractogram showed the crystalline nature of silver
nanoparticles, and the average crystallite size was found around 24.86 nm. The spherical shape of
morphology silver nanoparticles was observed in the SEM photograph. EDS spectrum showed the
high presence of silver. Synthesized silver nanoparticles exhibited antibacterial activity
against E.coli and S.aureus. This study reported the successful green synthesis of silver nanoparticles
implementing of plant extract.
Keywords: green synthesis, silver nanoparticles, sonication, Piper retrofractum, antibacterial

174
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4th ISOC (International Seminar on Chemistry)

Sri Fatmawati, M.Sc, Ph.D

4th ISOC (International Seminar on Chemistry)

Thank you for all of your attention. Enjoy the seminar!

With best wishes

4th ISOC (International Seminar on Chemistry)

4th ISOC (International Seminar on Chemistry)

4th ISOC (International Seminar on Chemistry)

Welcome Speech from Chairperson of ISoC 2020 ................................................. 1

4th ISOC (International Seminar on Chemistry)

4th ISOC (International Seminar on Chemistry)

4th ISOC (International Seminar on Chemistry)

4th ISOC (International Seminar on Chemistry)

4th ISOC (International Seminar on Chemistry)

4th ISOC (International Seminar on Chemistry)

4th ISOC (International Seminar on Chemistry)

4th ISOC (International Seminar on Chemistry)

4th ISOC (International Seminar on Chemistry)

Glycation is a nucleophilic reaction which leads to post-translational modifications of biomolecules (e.g.,

4th ISOC (International Seminar on Chemistry)

4th ISOC (International Seminar on Chemistry)

4th ISOC (International Seminar on Chemistry)

4th ISOC (International Seminar on Chemistry)

Keywords: PADs, microfluidic, paper, wax printing, quantitative analysis.

4th ISOC (International Seminar on Chemistry)

Keywords: Mesoporous Carbon, Activated Carbon, Ethylenediamine, Triethylenetetramine, CO 2,

4th ISOC (International Seminar on Chemistry)

Keywords: carotene, chromatographic separation, MS/MS analysis, xanthophyll

4th ISOC (International Seminar on Chemistry)

4th ISOC (International Seminar on Chemistry)

Key words: Diospyros lotus; phytochemicals, urease; reversion of multidrug resistant

4th ISOC (International Seminar on Chemistry)

4th ISOC (International Seminar on Chemistry)

Dichloro-diphenyl-trichloroethane (DDT) is a synthetic insecticide that widely used around the

Keywords: Bioremediation; Biodegradation; Pleurotus eryngii; Pseudomonas aeruginosa; DDT.

4th ISOC (International Seminar on Chemistry)

Keywords: antibacterial, toxicity, postpartum treatment, Indonesian herbal, LD50

4th ISOC (International Seminar on Chemistry)

Keywords: candlenut oil, epoxidation, poly ethylene glycol, polymerization, polyol

4th ISOC (International Seminar on Chemistry)

Keywords: Manganese, Manganese Ore, Leaching, Response Surface Method

4th ISOC (International Seminar on Chemistry)

Keywords: PVDF/LiCl membrane, Nannochloropsis sp. harvesting, TIPS, LiCl as additive

4th ISOC (International Seminar on Chemistry)

A proof-of-principle (prototype) device of desalination or salination process driven by AC-electricity

Keywords: ionic rectification, water desalination prototype, Nafion, desalination/salination process,

4th ISOC (International Seminar on Chemistry)

Keywords: Cracker, Contamination, Sea cucumber, Heavy metals, Kenjeran.

4th ISOC (International Seminar on Chemistry)

Liver cancer or scientifically known as Hepatocellular Carcinoma (HCC) is an uncontrolled growth

4th ISOC (International Seminar on Chemistry)

Keywords: amylose, sago starch, fractionation, edible film

4th ISOC (International Seminar on Chemistry)

Keywords: Activation, desilication, natural zeolite, gasoline

4th ISOC (International Seminar on Chemistry)

Keywords: Hydrogel, Chitosan, Nanoparticles, Fe/Fe oxide, NaBH4

4th ISOC (International Seminar on Chemistry)

Keywords: sago starch, cross link, sodium trimetaphosphate, FTIR

4th ISOC (International Seminar on Chemistry)

ZnO:Au nanoparticles were synthesized using eco-friendly substance of Imperatacylindrica L (ICL)

Keywords: Green synthesis, Nanoparticle,; ZnO:Au, Imperatacylindrica L

4th ISOC (International Seminar on Chemistry)

Keywords: surface chemistry, nanoporous carbon, gamma irradiation, specific capacitance,