PACSn

DATE

Oefine fcllowine demms

steilizeaion
Sterilizc1tion is rcess muim1 usedfo
kill l1 foems o f m i c a v o r q n i sna ss

sfores
This rotess s C u i e2d Out o mcuinttuîm
SAeile nviaommem +

fcsteuz iZertion &

cs tenizizcitiion i C dyfe of
SHerilfzeatiom which is used o kill e 1
e athagemic mica0 ca gomiSm.s
ese nd4 Ahe Food oducs.

3 mono chaome Stuinimg

momoComE traimim i5 also
Kmovm us Simple Suinin9
In which bucteriq Ce stuined
sin
usime Single Sfui'
Ex methylent blue ,Scutacmim.
 FAGE NO.

uwve) bucteaig
E:ShuPhylacotcus E sche ichfc
Stztptocacus. etc. Sclmomellc efC
( ASimg1e lufeareel 2 Adouble,lule-ed
mooth cellhwull WuvyCell wq
2Thickmessof cell Thickme ss_of Cel11
Wcll is 2o ho o . walls 8to o
momete umome er.
(32 Peptidogly um
a2 fePHHcloq lyceum
Tcuyes is cthica luy e r s a thi
layer /also Ccumbe luye/etem
my lAi faye ed. Simle Juyth-eo
C42 esence Of (9 Absemce o f
hichoic CAid hichoc_ciolds.

((S The oute

memreime 1s membume s
Cobsent esem
C62 meso some 1 5 C62 mesosormme s
more2 omimemt less komincmt:

C prd Comtemnt CE Liprd (emtemt

2o fo 30 /
C pepaly Sathuzide le?2 ipopaly scuchseid
Absem ese
3 Duw

PACE
DATE:
****

hutaclase
Sfeum

Vlve

Aljustble
Sufety vale

clumf
ressurf
9uwge

entoruted
hetallie basckef

Stainless Steel
hellow Vesse!

stumd
Jetnt

lectric
Supplq
 PAGE NO.:
DATE:

Hotair ovem

Ven

feim

Ceuse
Conti!m?

aséestrs
Jeske

Eotes
sea *
heik
Regilerte

doer
(4 fatlt feorm. PAGE M:
DATE

FTm Flurdhiogly olletde medie

cpm Soyer ben Caseim digest media

hertmote Om moist heaf SHeoilizetion

e y e of
msmoist heatStezilizeytiom
Het Steailizutiom
fart of
And Heat steilizetion is
Chysical eilizctioom methsd.

Steailizcution i s ome bt
mois hec
efective method or teilizutiom
the Vey-
for h e temS Whiclh Le Ccim

Sheailize 6 dyheat
CIato.cluve diffent locids
By rsim9
likeglass yntere ea-mems, abee
Stoppes md mis Cellemeoy s Ore

Steailizedcut "121 'clempacthur e Cl nd

15 Psi essuze.
uaeStecim 1S used for moistheci
Steailizatiom
Water ut Hish cssu leelis used
1 moist heeyt t e a i lizatton,
1 tis c m o e ertective mehod whe
Compured withdoy heer Steilfactiov.
Ahe hum CEMHccL medi.ce Sectos
siSMsed imthe steailizetiom of
Cressim9s,shee4s, Susgical aragnos tic
e pymems Comtuinens Comtrrmnu te
tems.
 PAGE NO. :

DATE:

meis+ hecat Cctn be used inSHeailizction

Cet diFfemt emprutuz
meisthectSBe»ilkizutiomsnulI)
involves Ahe se oF SHeum ut
121 1 3 ' .
meist hecf Ceambe Cctteqoaiz ed sote
fhree oups
Temotu e below loo
(i Temmputhot at loo'c
Ch) LEmpudLo e above oo c

cilTe. below lee'c

The aroce ss which. kills Call nom-S
Sfore
Tmimg cthogems bmilk 6
Subjectn9 the milK.o aembeture.
o f 6 3 'C rer 30 minytes or
1 3 e fe 20Secomd s Ts kmowm
S Pusteizctiom of milk

This
This method
umd + is
Kmowm csfusteal2cutioi
tvidel7 dppli ed i n dall-
tike mi1k amd burtea
ByHhis method 90mSiome mice
_ miS C e desdaoyed
Vcuccine Ccmbe epu ed im Vceimc 6ctt
temp. ot 6o' c e houre.
b usterizatiov, steuí.sed milk a
 PACE
DATE

m cut Joo 'c

Beilim t
mimteg kills ull VegetuHive
bueterid cme Some buctei cl Sor es
Thereeae +is mo ecomnended
or tea ili2ciomotinstumtnts
Sua4icca cedes
An edmmesp hee o. a e e stettma ís
sed Seile Coulire medic
which may decompos-e fE ubjected
to Highe temru e
A Koch or Armol ol SecimM
Steii2e
USull Sed
on_ iem media Cem net 6
SHeili2ed 6y this nethsd.
Tym dullis cfiom
iTemp.. abave Joo'c
Hect im tom ofkrtiru ted Stecm)uder
essures MOst dependluble cqqentor Ste
The Jub cautus desigmed Ao s e Stecimn

imd er cluted hecsue is Culted cutocluve

Sutputed stecm is c more etticient

ghelt2tm ctgent fhut h f u i beccise.

fupe emd Cloth woppers

e
 clusifices4ion of SteilreoHon
method. FAGE RO. TMeKk7
DATE

physiceal method
D Da hed S4eilizaiom
e.g Tncimeridion dizectluma 9
g e d he , ne cA etc
moist_heet SHeilizeHom
Eg asteiri zeitiom Ciatocluve
dllizutiom.
CKecdiaion lColdsfeailizcitiom
C 1 21e oéUV S UV ligh=
C2 40misim 9 ciclicom X-uN, -Ju,P**ay,
( f 1buion_mechcamicul methed
i Asbesto s AHea Seit2 flter)
ib smtcrcd 9lus.s SlHea (moaten
filea
2 El4e Gum dles cercim'c Filter)
Civ) memm baune fite ulHtou file
IChemicc method s
C cseous Steai2e14om
egtormeulodl ehyd e thylene onide
2

Byusim9 disnfectamfs
g Cese Pheno ettr
( haite dowm ineiple f foedure for
c Stuininq metho d m PACE n
acid-Fes t 3tui'ming mehod DATE
u

Krem-Stuimimo method
Kinciple Kocm Stuining isOne of he
mos t Jomkorteumt widel used
difhremtial Stuimim9 shi ch wus
ais Cove ed 6y chizistiam oum JnJ4.
Thisfechmifue divide bucteig jrho wo
Frou
CKrum- fositive:Those which getuín
Kimu Stui n \iKa Cmstt violet un d
urfeur_deep_yioletin Colour: c S.urêus
U12 CKumNeeutive Thosewhich lose h e
i m y Sfuin om capplicutHom ot decelouise
md tuxehe olour of 0 CounterSten
ike 5cufarumim o r
busie chsi..
-hey uPPec redj loloum x : E.celi

>Kumtuimim9 sefour difrremt Jeugemts

O Coy stul violet (BimuoStuim) The
Cystu iolet Stuim S us ed Fist amd
Stuims all Cells deep Viole fm
Calou
O mordamt(oum's Todime) mandemt
mub e dehmed cs um Suestmce thut
omsum Tmsoble Comlemd th
uim Semwes do ix Colouro bacteaiul cell.
O Decelourisin9 agemt Cethyl alohol, Js h)
Cel_ Wellof Um lositive ce Comtuiless
ipidoM uppl of decolouismg ugemt like
Clcoholor ucetone
ShaimKeE OEcellwall
tukes Place dveto dehfcha tiom f dec-ccuse
Perpmectbility for Cy-1-m9ibomucleute
(omplex
 (eumtcr Stuim (Sufau miv) This is frne
eugealt tu shui ded, those cells hut
huveb eem lerviousl dccolou1sed
Since onl CKcm-ve Cells u>e decolouised
they mcuy moh abso b (ouea stui
CCoumatre) Cetf actuin tht violef tolou
o imuy Stuin.
tvcedu>t
irepue asmeur oom cvided bucterial
Susiemsiom om decm 9reuse taee sli de.
Allow Smeur to urday f then heat Fix
uSuul mummex COve he Smeu b Cistl
viole Keep for mimte
ush the mcu with H,o f (ovez it t
CAum Lodime miaau te
WushSmtcr widh o de colouaize &ith
Clohol vey Cucult 4iu he ushina
doesnt Comtain vlef Celou
foT Noamma poeu 1o te Is Seccnd cne mevght
Kmse the Smeurwith Ho md unterstuim
wih Saf>umin o dilntted CLL bol u s eh si
for mnute
wush Smec with watex camd callew i
to uir do) R c chrep of eil athe
mec R xamime
imeY SiomObjecAve
 PAOE
UATE
Actd-fst Suiming metho
inciple Acid faxt Skuimin9 is CHmo4h er widel
ec diffr em tiul stuimi cedure i
bucteriolo
ThisStuin develoled by Puul Eholichin 13dz
ZiebI
Nee ls em imdepen dentl op31 eol cid
fust Auim iY_l8s2 )883 1S lormn17
vied
Seme bueteaicq desist decolouoiseudion 6 a t
CACe Cnd culcohol e t ekerred us Caeid
Faust eagamisms Acid Alcohol is ver
1tensive decolouo bed
histuimim9 divide bucteia ind twe rou?
2 Acid fust Cmdd
2 No- Acid fust
hisapcedue extemsivel) sed
digmess nmmubertaulosis m.lepacie.
Acid fstness ope1Letuin faycabuciea
of No cuedíu is(o2e/ce ted tui th hig

lipid Comtent
Dueto high 1id Cortent of el! ve. L, cicie

Fast cells hu & Yeleativel low lmecat.

t dye f i_diffiuult oStuin.
for Staimimo of buct eiu fenetau tio O
with S ees
Kaima
aimur)dye is fucili uted interSiFiE
PhenelLwhich.ees cs ehemitea!

Heut also vsed acts u s fhysicul j 27t eus.me

Omee these cells ue Stuime, +is
o decoloua ise i t h aci d
diffic l
cumdalohol.
 PAGE RO. :
DATE:

The Aid fast SAuimne G) difent eugzet

Bimu-y stui (cun bol fuchsi )
Cunbol fuchs Phemolie Stuir Thut-
Jehible in lipoidal material tohich
(Onstitufe major foatiom of myobucteiu1
Cell wall_ callows lemeitiom etomdig
of red Staim
Al ceNs ckfeco xed in Colou atter
Pp] of
mun Stum
Decelorizn9- CuemE Cuud-uteohal er lsÍ Hs
Smec is Coeled be foae decolauaisationa
which allow wuxy cel Substuomce to hnden
om cafpmDf decolouismq semt_LidauT
CellsShows Tsistumce o decelaLLisuhlcL
Sun moreSoubl e im cellulur

Waxes Ahum deelouri

sim tues emt
maoyStuin u i l ) emmoed duin9
duing
eolouisutim monc ueid u t cels
Obsemved as Coleuless.

(omter Stuim (methylene

blue)
(3
(3 bucteiqubsa6 taR
on uid fust
Coumterstuim CAPpeCLY CAS ble ox
Co lour Acid ust cel|
while
ot imu
n e e d tolau
Stui
 PACE
DATE

fhecedye

epcre c meu o vem

uspemsom
vem ucdoria
Allo Smeu o cr day t h en hev
for Heed Smeu With Cusbelfuchsi
Stuin heat SLde hem belowl1
m ss_ minutes
Domt boil Hh& Stiy f_Cnsure hu
Stuir doegm dey Out
Allow slide 4s Ceol mimutes Pesent he
reuKudge oEE slidzin ubs EgNent Sar
wasa i t h tu? wute
Oscolois a Hae Slide wing acd-alcohel
umil Curb« fuchsim ruils e weh iem
meur
wush w i t h wuter*Coumte Srin
wit C GMeus SoM oF muleuc hi+
Sreem0 methylem_ue t mmie
wash_Smeur tul wuter dm cimd
ummim umdey e i l i m m ê a s i e
OiecA
 FAGE O.
DAT

Jher tnete on sderilityndictos

mdiccrtim9o momihuring DE SHerii2ciDy
ecess cu eachieved 6y we of hysia1
chemitul obiological Tndicurtorz ok he
ht
Seilr tiom fesfermumte
OPhysice ZmdicuhorS
d moi's heet
het
R u d io SAeni lizatim
v us deus_me-ta
filhutim
( chemice Tndiceter
2 Browme's tubes
cii2 Lvihme ss hibes
( HectSemsitve fu le
oy) Koy e Sceehct
chemiccl dosimete
Biclo9icul Tndicuto