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3 2 1
TRANSLOCATION OF
MATERIALS
PLATETT

100 001 067 256

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CONTENTS
TOPIC 1: TOPIC 2: TOPIC 3:
ll.Translocation through ll.Translocation through ll.Translocation through
an artificial membrane an artificial membrane the cell membrane
a. Osmosis b. Dialysis a. Animal Cell
What is Translocation?
Act, process, or an instant of changing location or position:such
as the conduction of soluble material(such as metabolic product)
from one part to another.
CONTENTS
TOPIC 4: TOPIC 4: TOPIC 3:
ll.Translocation through ll.Translocation through ll.Translocation through
the cell membrane the cell membrane the cell membrane
b. Plant Cell c. Diffusion d. Surface Tension
Translocation through an
Artificial Membrane
A. OSMOSIS
MATERIAL: Materials:
thistle tube
beaker
rubber band
iron stand
burette clamp
yema wrapper
table salt
table sugar
Procedure:
1.Fill the bulb of a thistle tube with a saturated salt
solution up to its constricted portion.
2.Cover the thistle tube with yema wrapper and secure it
with a rubber band.
3.Immerse the bulb of the thistle tube in a big beaker with
water suspending it by means of a burette clamp to an
Iron stand.
4.Be sure that the levels of both liquids are the same.
5.Observed the level of the solution inside the thistle tube
after an hour.
6.Repeat the same procedure using sugar solution.
7.Observe.
Result:
Since the solution is unsaturated it contains less than the
maximum amount of solute that is capable of being
dissolved. After an hour of observation, the group noticed
that the salt dissolved and formed a
homogeneous solution. Similar to the observation of
sugar, because sugar is a solid solute with a liquid
solvent, which is water, the sugar dissolved as it
distributed uniformly throughout the water.
Translocation through an
Artificial Membrane: Dialysis
Materials :
Yema wrapper
Rubber band
Starch pastpaste
Iodine
Glucose
Benedict’s solution
Benedict’s Test Reagent Result :
Benedict's reagent begins as an aqua-blue liquid. It turns
yellow to orange when heated in the presence of reducing
sugars. The higher the concentration of reducing sugar,
the "hotter" the final color of the reagent. Blue to blue-
green or yellow-green is often considered negative,
yellowish to bright yellow is considered very positive, and
bright orange is considered quite positive.
Translocation through the
cell membrane: Animal Cell
A. Animal Cell
Materials:
Citrated blood, test tube, test tube rack, glass slide,
microscope, black thread, medicine dropper, isotonic,
hypotonic, and hypertonic solutions.
Procedure:
Procedure:
1.Place a 1ml of isotonic, hypotonic, and hypertonic
solutions in each (3) test tubes.
2.Place these test tubes on test tube rack and stretch
black thread across the back of the test tube rack.
3.Add a two (2) drops of blood in each test tube and
determined the time for hemolysis to take place. This is
indicated by the black thread seen through the solutions.
Result
Isotonic solution - Osmolality of the both fluids is equal.
As such, though water diffuses in and out, there’s no net
change in the volume of cell.
Hypertonic solution- the cell has a lower osmolarity than
the extracellular fluid, water will leave the cell. As a
result, the cell shrinks, a process known as plasmolysis.
Hypotonic solution- Water travels from a low osmolarity
zone to a high osmolarity region. Because the
extracellular fluid has a low osmolarity, water would rush
into the cell in this situation.The cell would expand and
eventually burst.
SLIDE A
Result SLIDE B

RHOEO LEAF RHOEO LEAF


WITH WATER WITH SALT
Translocation through the cell membrane:Diffusion
Materials: 4.Allow the water
inside the beaker to 6.Note the time 7.Observe .
Beaker required for the 8.Repeat the
KMnO4 Crystal become very still.
5.Carefully place colored ions to procedure by
Intermediate pad travel 1 cm along a heating the water
several crystals of
Procedure: KMnO4 at the line towards the inside the beaker to
1.Place 20 mL water bottom of the beaker center. 800 C.
into a beaker. where the lines are
2.Place the beaker perpendicular to the
over a piece of beaker edge.
intermediate pad.
3.Get the
temperature of the
water in the beaker.
RESULTS:
Materials: 7.Observe .
Beaker 8.Repeat the
KMnO4 Crystal At 800C (Hot Water) - The crystals of KMnO4 spread faster in
procedure by
Intermediate pad water at higher temperatures, indicating that temperature
heating the water
influences the speed with which molecules spread from higher
Procedure: inside the beaker to
to lower concentrations until they achieve equilibrium.
1.Place 20 mL water 800 C.
into a beaker. At room temperature – The crystals of KMnO4 spreads slowly
2.Place the beaker to the water in the beaker .
over a piece of
intermediate pad.
3.Get the
temperature of the
water in the beaker.
Translocation through the cell membrane:Diffusion
Materials: 4.Allow the water
inside the beaker to 6.Note the time 7.Observe .
Beaker required for the 8.Repeat the
KMnO4 Crystal become very still.
5.Carefully place colored ions to procedure by
Intermediate pad travel 1 cm along a heating the water
several crystals of
Procedure: KMnO4 at the line towards the inside the beaker to
1.Place 20 mL water bottom of the beaker center. 800 C.
into a beaker. where the lines are
2.Place the beaker perpendicular to the
over a piece of beaker edge.
intermediate pad.
3.Get the
temperature of the
water in the beaker.
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