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3 2 1 TRANSLOCATION OF MATERIALS PLATETT
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CONTENTS TOPIC 1: TOPIC 2: TOPIC 3: ll.Translocation through ll.Translocation through ll.Translocation through an artificial membrane an artificial membrane the cell membrane a. Osmosis b. Dialysis a. Animal Cell What is Translocation? Act, process, or an instant of changing location or position:such as the conduction of soluble material(such as metabolic product) from one part to another. CONTENTS TOPIC 4: TOPIC 4: TOPIC 3: ll.Translocation through ll.Translocation through ll.Translocation through the cell membrane the cell membrane the cell membrane b. Plant Cell c. Diffusion d. Surface Tension Translocation through an Artificial Membrane A. OSMOSIS MATERIAL: Materials: thistle tube beaker rubber band iron stand burette clamp yema wrapper table salt table sugar Procedure: 1.Fill the bulb of a thistle tube with a saturated salt solution up to its constricted portion. 2.Cover the thistle tube with yema wrapper and secure it with a rubber band. 3.Immerse the bulb of the thistle tube in a big beaker with water suspending it by means of a burette clamp to an Iron stand. 4.Be sure that the levels of both liquids are the same. 5.Observed the level of the solution inside the thistle tube after an hour. 6.Repeat the same procedure using sugar solution. 7.Observe. Result: Since the solution is unsaturated it contains less than the maximum amount of solute that is capable of being dissolved. After an hour of observation, the group noticed that the salt dissolved and formed a homogeneous solution. Similar to the observation of sugar, because sugar is a solid solute with a liquid solvent, which is water, the sugar dissolved as it distributed uniformly throughout the water. Translocation through an Artificial Membrane: Dialysis Materials : Yema wrapper Rubber band Starch pastpaste Iodine Glucose Benedict’s solution Benedict’s Test Reagent Result : Benedict's reagent begins as an aqua-blue liquid. It turns yellow to orange when heated in the presence of reducing sugars. The higher the concentration of reducing sugar, the "hotter" the final color of the reagent. Blue to blue- green or yellow-green is often considered negative, yellowish to bright yellow is considered very positive, and bright orange is considered quite positive. Translocation through the cell membrane: Animal Cell A. Animal Cell Materials: Citrated blood, test tube, test tube rack, glass slide, microscope, black thread, medicine dropper, isotonic, hypotonic, and hypertonic solutions. Procedure: Procedure: 1.Place a 1ml of isotonic, hypotonic, and hypertonic solutions in each (3) test tubes. 2.Place these test tubes on test tube rack and stretch black thread across the back of the test tube rack. 3.Add a two (2) drops of blood in each test tube and determined the time for hemolysis to take place. This is indicated by the black thread seen through the solutions. Result Isotonic solution - Osmolality of the both fluids is equal. As such, though water diffuses in and out, there’s no net change in the volume of cell. Hypertonic solution- the cell has a lower osmolarity than the extracellular fluid, water will leave the cell. As a result, the cell shrinks, a process known as plasmolysis. Hypotonic solution- Water travels from a low osmolarity zone to a high osmolarity region. Because the extracellular fluid has a low osmolarity, water would rush into the cell in this situation.The cell would expand and eventually burst. SLIDE A Result SLIDE B
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WITH WATER WITH SALT Translocation through the cell membrane:Diffusion Materials: 4.Allow the water inside the beaker to 6.Note the time 7.Observe . Beaker required for the 8.Repeat the KMnO4 Crystal become very still. 5.Carefully place colored ions to procedure by Intermediate pad travel 1 cm along a heating the water several crystals of Procedure: KMnO4 at the line towards the inside the beaker to 1.Place 20 mL water bottom of the beaker center. 800 C. into a beaker. where the lines are 2.Place the beaker perpendicular to the over a piece of beaker edge. intermediate pad. 3.Get the temperature of the water in the beaker. RESULTS: Materials: 7.Observe . Beaker 8.Repeat the KMnO4 Crystal At 800C (Hot Water) - The crystals of KMnO4 spread faster in procedure by Intermediate pad water at higher temperatures, indicating that temperature heating the water influences the speed with which molecules spread from higher Procedure: inside the beaker to to lower concentrations until they achieve equilibrium. 1.Place 20 mL water 800 C. into a beaker. At room temperature – The crystals of KMnO4 spreads slowly 2.Place the beaker to the water in the beaker . over a piece of intermediate pad. 3.Get the temperature of the water in the beaker. Translocation through the cell membrane:Diffusion Materials: 4.Allow the water inside the beaker to 6.Note the time 7.Observe . Beaker required for the 8.Repeat the KMnO4 Crystal become very still. 5.Carefully place colored ions to procedure by Intermediate pad travel 1 cm along a heating the water several crystals of Procedure: KMnO4 at the line towards the inside the beaker to 1.Place 20 mL water bottom of the beaker center. 800 C. into a beaker. where the lines are 2.Place the beaker perpendicular to the over a piece of beaker edge. intermediate pad. 3.Get the temperature of the water in the beaker. THANK TOT