AP Biology Practice Enzyme FRQ Last Name: _________ First Name: ________ Per.

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a. Graph the data on the axes provided and calculate the rate of the reaction for the time period 0 to 30 minutes.

b. Explain why a change in the reaction was observed after 30 minutes.

c. Draw and label another line on the graph to predict the results if the concentration of the enzyme was doubled.
Explain your predicted results.

d. Identify TWO environmental factors that can change the rate of an enzyme-mediated reaction. Discuss how
each of those two factors would affect the reaction rate of an enzyme.
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An investigation was performed to determine the rate of oxygen released from the decomposition
(breakdown) of hydrogen peroxide by the human enzyme catalase. The investigation was performed at two
different temperatures; the data is presented in the table below. Normal human body temperature is 37 ⁰C.

Time Amount of Amount of

(minutes) Oxygen Oxygen
released released
(ml) at 20⁰C (ml) at 10⁰C
0 0 0
1 20 10
2 40 20
3 50 30
4 55 40
5 58 47
6 58 55
7 58 58

a. Graph the data, include: labels and units for the x & y axes, keep the scale consistent, correctly graph
the data & label the two lines or include a key. 5 pts.

b. Calculate the rate of reaction for the 20⁰C treatment between 0 and 2 minutes and explain this rate. 2
pts.

c. Calculate the rate of reaction for the 20⁰C treatment between 5 and 7 minutes and explain this rate. 2
pts.

d. Describe and explain the relationship between temperature and rate of enzyme-catalyzed reaction
shown in the data presented. 2 pts.

e. Add (and label) a line to the graph for what the reaction would look like at 37⁰C. 1 pt.
a. 1 pt. for x-axis being time, unit = minutes, scale interval consistent
1 pt. for y-axis being oxygen released, unit = ml, scale interval consistent
1 pt. for correctly graphed 20⁰C data points and smooth curve
1 pt. for correctly graphed 10⁰C data points and smooth curve
1 pt. for labels or a key
b. 1 pt. for calculation: (40 ml – 0 ml)/ (2 min – 0 min) = 40 ml/2 min. = 20 ml/min
1 pt. for explanation: This represents the highest rate of the reaction because
the substrate is not limited.
c. 1 pt. for calculation: (58 ml – 58 ml)/ (7 mi. – 5 min) = 0 ml/min
1 pt. for explanation: The rate is zero because the enzyme ran out of substrate.
d. 1 pt. for description: The rate of the enzyme-catalyzed reaction increased in
the 20⁰C as compared to the 10⁰C temperature treatment.
1 pt. for explanation: The reason for this is that increasing heat increases
molecular motion such that the rate of collision between enzyme and
substrate is increased, thus increasing the rate of reaction.
e. 1 pt. for adding a labeled line to the graph (see graph above) that has a steeper
slope than the 20⁰C and levels off to a rate of zero after 58 ml of oxygen was
released.