Dr.

Bhatia Medical Coaching Hyderabad

Bio-Chemistry – T& D.
Face to Face Date :- 18-12-2021
1. A person presented with diarrhoea, dementia dermatitis (typical castle neck appearance) this patient
may have

A. B3 deficiency
B. B6 deficiency
C. Hartnup disease
D. Carcinoid syndrome

2. Identify the heteropolysaccharide from the disaccharide repeat unit given in the picture

A. Heparan sulphate
B. Heparin
C. Hyaluronic acid
D. Dermatan sulphate

3. A 12 years old intellectually disabled boy having short stature, protuberant abdomen with umbilical
hernia, prominent forehead. His vision is normal. His parents are normal. What is metabolic defect
in this disorder?
A. L-iduronidase
B. Iduronate sulfhatase
C. Aryl Sulfatase B
D. Beta Glucoronidase

4. A 2 month old infant presented with generalized muscle weakness with a “ floopy infant “
appearance. He also has other features like macroglossia, feeding difficulty, hepatomegaly and
hypertrophic cardiomyopathy. What is the diagnosis?
A. Type I GSD
B. Type II GSD
C. Type III GSD
D. Type IV GSD
5. A 6-months old child presented with fasting hypoglycemia, hepatomegaly, growth retardation and.
Once this child reached puberty, he no longer has hypoglycemia, muscle weakness, hepatomegaly.
He reached normal adult height also. What is the most probable diagnosis?

A. Type I GSD
B. Pompe’s disease
C. Coris disease
D. Andersen disease

6. Identify the correct regulatory molecules:

A. A= Glucagon; B=insulin
B. A=epinephrine; B=Glucagon
C. A= Epinephrine; B= Insulin
D. A= insulin; B= Glucagon

7. A 2 years old boy presented with fasting hypoglycemia, hepatomegaly, doll like facies and thin
extremities. Kidneys are enlarged but no splenomegaly. What is the most probable GSD?

A. Type 1 GSD
B. Type II GSD
C. Type III GSD
D. Type IV GSD
8. A 3 weeks old neonate who began vomiting 2 days after birth, usually with in 30 minutes after
breastfeeding. He also has abdominal distension with enlargement of liver, with jaundice. The
consulting doctor did two urine dipstick test, one specific for glucose was negative, second test
specific for reducing sugar was positive. What is the diagnosis?

A. Hereditary fructose intolerance

B. Classic galactosemia
C. Essential fructosuria
D. Von gierkes disease

9. Routine examination of the urine of an asymptomatic patient showed a positive reaction with
benediet test (a copper reduction method of detecting reducing sugars). But a negative reaction with
the glucose oxidase test. Which one of the following sugars is least likely t be present (assuming a
single elevated saccharide)?
A. Lactose
B. Fructose
C. Sucrose
D. Galactose

10. True of isoenzymes?

A. Same rate of work, catalize same reaction and have similar kinetics
B. Same quaternary structure
C. Same group of classification used
D. Have similar distributions in organs

11. Which one of the following statements concerning glycolysis is correct?

A. Hexokinase is important in hepatic glucose metabolism only in the absorptive period following
consumption of a carbohydrate containing meal.
B. Fructose 2.6 Bisphosphate is a potent inhibitor of phosphofructokinase.
C. The regulated reactions are also the irreversible reactions
D. The conversion of glucose to lactate yields two ATP and two NADH.

12. Which reaction in the figure below occurs in both muscle and liver but has substantially different
qualities in the two?
 A. Reaction A
B. Reaction B
C. Reaction C
D. Reaction D

13. Enzymes involved in Gluconeogenesis:

a) Pyruvate kinase
b) PEP carboxy kinase
c) Pyruvate carboxylase
d) Glyceraldehyde 3 P dehydrogenase
Which of the following enzymes are involved in gluconeogenesis?
A. 1 and 2
B. 2 and 3
C. 2,3,4
D. 1,2,3

14. A 3 years old man presented with symptoms of weakness, fatigue, shortness of breath, and dizziness.
His hemoglobin level was less than 7 g dl (normal for male being greater than 13.5 g/dl) red blood
cells isolated from patient showed abnormally low level of lactate production. A deficiency of which
one of the following enzymes would be the most likely cause of this patient’ s anemia?
A. Phosphoglucose isomerase
B. Phosphofructokinase
C. Pyruvate kinase
D. Glucose -6- phosphate dehydrogenase

15. A middle-aged woman presented with fissures in mouth, tingling sensation. Investigation showed
reduced glutathione reductase activity. Which vitamin deficiency causes this?
A. Vitamin B1
B. Vitamin B2
C. Vitamin B6
D. Vitamin B12

16. A 48 years old male with excessive drinking over an extended period of time while eating poorly,
admitted to the hospital with” high output” heart failure. Which of the following enzymes is most
likely inhibited?
A. Isocitrate dehydrogenase
B. ∝- ketoglutarate dehydrogenase
C. Succinate thiokinase
D. Citrate synthase

17. Thiamine level is best monitored by (recent update)-

A. Transketolase level in blood
B. Thiamine level in blood
C. G6PD activity
D. Reticulocytosis

18. Biological role of metallothionein is to sequester harmful metal ions. These bind: -
A. CD++, Cu++ and Zn++
B. A1+++, Hg++ and NH4+
C. Pt+++, As+++ and PO4
D. Fe+++, Na+ and K+
19. A buffer that is most effective at PH of about 8.3 is: -
A. Acetate buffer
B. Bicarbonate buffer
C. Phosphate buffer
D. Tris buffer

20. A low-level carbon dioxide labeled with C14 is accidentally released into the atmosphere surrounding
industrial worked as they resume work following the lunch hour. Unknowing, they breathe the
contaminated air for 1 hour. Which of the following compounds will be radioactively labeled?
A. All of the carbon atoms of newly synthesized fatty acid
B. About one half of the carbon atoms of newly synthesized fatty acids
C. About one third of the carbons of newly synthesized malonyl CoA
D. About one 4th of the carbons of newly synthesized malonyl CoA

21. A A 6-months old boy was hospitalized following a seizure. History revealed that for several days
prior, his appetite was decreased due to a "stomach virus". At admission, his blood glucose was 24
mg/dl (age-referenced normal is 60-100). His urine was negative for ketone bodies, but positive for a
variety of dicarboxylic acids. A tentative diagnosis of medium-chain fatty acyl CoA dehydrogenase
(MCAD) deficiency is made. In patients with MCAD deficiency, the fasting hypoglycemia is a
consequence of:
A. Decreased acetyl CoA production
B. Decreased ability to convert acetyl CoA to glucose
C. Increased conversion of acetyl CoA to acetoacetate
D. Increased production of ATP and NADH

22. When the liver is actively synthesizing fatty acids, a concomitant decrease in β oxidation of fatty
acids is due to which of the following?
A. Inhibition of a translocation between cellular compartments
B. Inhibition by an end product
C. Activation of an enzyme
D. Detergent in adipocyte lipolysis

23. A 4 years old boy of middle-eastern ancestry is known to have inherited favism a sensitivity to fava
beans due to glucose-6 – phosphate dehydrogenase deficiency (MIM305900). Which of the
following indicates the primary pathway affected by this deficiency and the pathway secondarily
affected because one of its substrates will be deficient?
A. Pentose phosphate pathway fatty acid synthesis
B. Pentose phosphate pathway fatty acid oxidation
C. Citric acid cycle, Pentose phosphate pathway
D. Citric acid cycle glycolysis

24. Abetalipoproteinemia is a genetic disorder characterised by malabsorption of dietry lipids

steatorrhoea, accumulation of intestinal triglcerides and hypolipoproteinemia. A deficiency of
which apoprotein would account for this clinical picture?
A. apo B 100
B. apo B 48
C. apo CII
D. apo E
25. A 7-years old boy presented with severe abdominal pain. On examination, he had xanthomas.
Blood sample was taken for work-up, blood sample had milky appearance of plasma. Which of the
following lipoprotein is increased?
A. LDL
B. HDL
C. Chylomicron
D. Chylomicron remnants

26. Clinical Picture Is Pathognomonic of Which Dyslipidemia?

A. Abetalipoproteinemia
B. Tangier’s Disease
C. Fish Eye Disease
D. Sitosterolemia

27. A 30-years- old male patient came to medicine opd for swelling which looks like grapes, he said
initially it was small cluster of swelling which later increased in size. he also complained of
yellowish pigmentation of creases of palms. his fasting lipid profile was done, both cholesterol and
triacycerils level was elevated. what is the diagnosis?

A. Familial Chylomicronemia Syndrome

B. Familial Defective Apo B
C. Sitostrelomia
D. Familial Dysbetalipoproteinemia

28. On laboratory investigations in a patient, LDL was highly elevated but the level of LDL receptors was
normal. Which of the following is most probable cause?
A. Phosphorylation of LDL receptors
B. Lipoprotein lipase deficiency
C. Apo B-100 mutation
D. Cholesterol Acyl Co-A transferase deficiency
 29. The recently discovered protein, proprotein convertase subtilisin/kexin type 9 (PCSK9), has been
identified as a potential target for antiatherogenic drugs BECAUSE:
A. It decreases the number of LDL receptors exposed at the cell surface, thus LDL uptake is lowered
and blood cholesterol levels rise.
B. It inhibits the binding of apo B to the LDL receptor, thus blocking uptake of the lipoprotein and
raising blood cholesterol levels.
C. It increases the absorption of cholesterol from the intestine.
D. It prevents the breakdown of cholesterol to bile acids in the liver.

30. In this is figure of mitochondria location of enzymes are pointed by A, B, C. For each item listed
below, select the appropriate location from the drawing shown. Select the one best answer

A. Carnitine shuttle – B
B. F0F1 ATP synthase – C
C. HMG – CoA lyase – B
D. Carnitine acyltransferase -1 - A

31. The reaction: Acetoacetate + Succinyl CoA →Acetoacetyl CoA + succinate occurs in all of the
following EXCEPT
A. Brain
B. Striated muscle
C. Liver
D. Cardiac muscle

32. Niemann-Pick disease, like other neurolipidoses, present in infancy or childhood with plateauing of
development and neurologic regression. The accumulating substance is a phospholipid made in
which of the following steps in the figure below?

A. Step D
B. Step A
C. Step B
D. Step C
33. The following histopathological picture is seen on bone marrow examination. What is the likely
diagnosis?

A. Fabry’s Disease
B. Gaucher’s Disease
C. Niemann Pick Disease
D. Sandhoff’s Disease

34. Which of the following enzymes has decreased activity in fasting except?
A. Glucokinase
B. Hormone sensitive lipase
C. Glycogen synthase
D. Acetyl CoA carboxylase

35. In a diabetic patient

A. RQ decreases and on administrasion of insulin RQ increases
B. RQ increases and on administrasion of insulin RQ decreases
C. No effect of insulin on RQ
D. No effect of diabetes on RQ

36. Which one of the following statements about protein structure is correct?
A. Proteins consisting of one polypeptide can have quaternary structure
B. The formation of a disulfide bond in a protein requires that the two participating cysteine residues
be adjacent to each other in the primary sequence of the protein
C. The stability of quaternary structure in proteins is mainly a result of covalent bonds among the
subunits
D. The Information required for the correct folding of a protein is contained in the specific sequences
of amino acids along the ploypetide chain.

37. The peptide ala-arg-his-gly-glu is treated with peptidases to release of the amino acids. The
solution is adjusted to pH 7, and electrophoresis is performed. In the electrophoretogram depicted
below, the amino acid indicated by the arrow is most likely to be.
A. Glycine
B. Arginine
C. Glutamate
D. Histidine

38. Most accepted method for HbA1cC estimation is

A. Affinity chromatography
B. Ion exchange chromatograprhy
C. Mass spectrometery
D. Xray crystallography
39. FIGLU is a metabolite of –
A. Folic acid
B. Histidine
C. Tyrosine
D. Alanine

40. In a patient with cystic fibrosts caused by the AF508 mutation, the mutant cystic fibrosis
transmembrane conductance regulator (CFTR) protein folds incorrectly. The patient's cells modify
this abnormal protein by attaching ubiquitin molecules to it. What is the fate of this modificd CFTR
protein?
A. It performs its normal function, as the ubiquitin largely corrects for the effect of the mutation
B. It is secreted from the cell
C. It is placed into storage vesicles
D. It is degraded by the proteasome

41. PKU is a congenital aminoacid metabolic disorder.In one of the following rare variants of PKU The
enzyme deficient is
A. Histidine decarboxylase
B. Phenylalanine Hydroxylase
C. Dihydropterin Reductase
D. Tyrosine Kinase

42. A cardiovascular researcher is conducting a study to evaluate the relationship between folate levels
and cardiovascular disease. The results of the study show that persons with lower folate levels
have twice the risk of cardiovascular disease mortality than do those with higher folate levels.
Which of the following enzymes is most closely related to the role of folate in lowering the risk of
cardiovascular disease?
A. Dihydrofolate reductase
B. Glycinamide ribonucleotide transformylase
C. Homocysteine methyltransferase
D. Ribonucleotide reductase

43. What is the structure indicated below?

A. Guanosine
B. Purine
C. Pyrimidine nucleoside
D. Purine nucleoside
44. True about APO-B-48 & APO-B-100 is
A. APO-B-48 and APO-B-100 are made up of 48 and 100 amino acids respectively
B. APO-B-48 and APO-B-100 are of molecular mass 48 and 100 kDa respectively
C. APO-B-48 has 48% mass of APO-B-100
D. APO-B-48 and APO-B-100 are required for LDL and chylomicrons respectively

45. A mutation to this sequence in eukaryotic mRNA will affect the process by which the 3'-end poly-A
tail is added to the mRNA.
A. CAAT
B. GGGGCG
C. AAUAAA
D. TATAAA

46. Taql is a restriction endonuclease commonly used to cut human DNA into pieces before inserting it
into plasmid. Which of the following is most likely to be the recognition sequence for this enzymes.
A. AAGG
B. AAGA
C. GGCC
D. TAAT

47. Telomeres are complexes of DNA and protein that protect the ends of linear chromosomes. In most
normal human somatic cells, telomeres shorten with each division, in stem cells and in cancer cells,
however, telomeric length is maintained. In the synthesis of telomeres:
A. Telomerase, a ribonucleoproteein. provides both the RNA and the polymerase needed for
synthesis.
B. The RNA of telomerase serves as a primer.
C. The polymerase of telomerase is a DNA-directed DNA polymerase.
D. The shorter. 3'-»5' strand gets extended

48. The base sequence of the coding strand of DNA used for transcription, has the base sequence GTG.
What is the base sequence of RNA product?
A. GTG
B. GUG
C. CAC
D. GAG

49. In the following partial sequence of mRNA, a mutation of the template DNA results in a change in
codon 91 to UAA. What type of mutation is it?

A. Silent
B. Nonsense
C. Missense
D. Frame shift
50. Cytosolic Cytochrome C mediates
A. Apoptosis
B. Electron transport
C. Krebs cycle
D. Glycolysis

51. Which is true about histone acetylation

A. Increased euchromatin formation
B. Increased heterochromatin formation
C. Methylation of DNA
D. DNA replication

52. Mechanism involved in RNA interference is

A. gene knock down
B. Gene knock in
C. RNA editing
D. protein editing

53. A culture of E.coli is grown in a medium containing glucose and lactose. The expression of the
lactose operon over time in the cells is shown in the graph below. Which statement best describes
the change that occurred t point A?

A. Lactose was added to the culture

B. cAMP concentration increased in the cells
C. Repressor protein dissociated from the operator
D. Repressor protein became bound to the operator

54. To determine the global patterns of gene expression in two different types of tumour cells. Which
of the following techniques would be most appropriate for this purpose: -
A. Southern blot
B. Northern blot
C. Western blot
D. Microarray

55. The DNA sequence to be amplified by PCR is shown below. the dashed line indicates the internal
sequence where the potential mutation is located. Which answer choice represent the pair of
primers that should be used in PCR
5TCCTGAGC-------------------AAATGTGT3
3AGGACTCG------------------TTTACACA5
A. AGGACTCG and TTTACACA
B. GCTCAGGA and AAATGTGT
C. GCTCAGGA and ACACATTT
D. TCCTGAGC and ACACATTT
56. A 24 years old female is admitted for observation 3 days after contacting a flu-like illness.
Laboratory studies includes an ammonia level of 400 mol/l (normal 0-35). With reference to the
urea cycle diagram below, and given that ornithine transcarbamoylase is mutatd select the option
that indicates the compound deficient in this female and the compound derived from condensation
of CO2 and NH4+?

A. Compound A deficient, compound E from CO2 and NH4+

B. Compound B deficient, compound E from CO2 and NH4+
C. Compound C deficient, compound A from CO2 and NH4+
D. Compound C deficient, compound B from CO2 and NH4+