1. Which of the followings CANNOT be produced by the process of fermentation?

A. NAD+
B. NADH
C. Alcohol
D. Lactic acid

2. In Gal4/UAS system, the target cell type to induce ectopic gene expression can be
selected, by changing
A. Promoter of Gal4
B. Gal4 gene
C. UAS sequence
D. Transgene regulated by UAS

3. CRE recombinase cannot be used in

A. Creating conditional knockout mice
B. Cell-type specific transgene expression
C. Expression of GFP
D. Forward genetic screening

4. Which of the followings are NOT related to Tet-off system?

A. loxp
B. Doxycycline
C. Inducible expression
D. Reversible

5. Epitope tags can be used in

A. Knockdown
B. Affinity purification
C. Knockout
D. Mutagenesis

6. When AAV virus is made in mammalian cell culture, which of the followings is
correct?
A. The original AAV genome contains E2A and E4 which is required for packaging.
B. The gene flanked by ITRs will be included in the virus particle.
C. The genes are packaged in the form of RNA.
D. Plasmid DNAs transfected to the cell will included in the virus particle.
7. When the gene of interest is ubiquitously expressed, but you want to know the role
of the gene only in the germ line cells, which of the followings is not suitable to
conduct?
A. Conditional knockout
B. Tissue specific knockdown
C. Tissue specific overexpression
D. Constitutive knockout

8. Good model organisms should meet at least some of the criteria except
A. Easy to breed
B. Having a special ability
C. Having a special diability
D. Cute

9. Which of the followings is NOT in 3R in animal research?

A. Replacement
B. Reproducible
C. Refinement
D. Reduction

10. Pluripotent stem cells can be

A. Found in the fibroblast
B. Directly used to give birth of human child
C. Created by introducing transcription factors
D. Used for only research, but not for the tissue regeneration by the ethical issue

11. siRNA screening can be easily done

A. in living mice
B. by mutagenesis using EMS
C. by temperature sensitive mutants
D. with barcoding the construct

12. To search for an interacting protein, which of the following can be used?
A. Affinity purification followed by LC-MS/MS analysis
B. LC-MS/MS analysis using the cell extract
C. Transcriptome analysis
D. Single cell RNA-sequencing
13. Gain of function phenotype cannot be obtained by
A. Overexpression
B. Dominant mutation
C. Recessive mutation
D. Ectopic expression

14. Optogenetics uses

A. Light
B. Optomotor response
C. Optimization of the chemical compounds
D. Optical tweezers

15. Genome sequencing to discover the disease-related mutation is usually done with
A. Blood cells for somatic mutation
B. Blood cells for germ-line mutation
C. Hairs for somatic
D. Nails for germ-line mutation

16. Polyclonal antibodies are good in terms of

A. Reproducibility
B. The use of hybridoma
C. Containing various antibodies recognizing the same antigen, giving good signals
D. Easy-purification from bacteria

17. In Drug development, there are phase I to IV. Phase II is

A. with model animals
B. with healthy human subject
C. with large number of patients
D. with limited number of patients

18. Immunotherapy targeting to PD1 is not related to

A. Tumor
B. T-cell
C. Gene therapy
D. monoclonal antibody
19. Transplanted organ cannot be rejected by the function of
A. T-cell
B. Immunosuppression
C. MHC (Major Histocompatibility Complex) genes
D. HLA (Human Leukocyte Antigen) genes

20. Informed consent can be obtained in the case

A. Only when a doctor delivers the necessary information in writing.
B. When the patients are forced to agree with the operation.
C. When the patients do not understand the operation.
D. When a doctor delivers the necessary information verbally.