Dr.

Bhatia Medical Coaching Institution Hyderabad

Bio- Chemistry – 2
April Batch , Date :- 04-01-2022

1. Which of the following are not derived from tyrosine?

7. A 4-year old boy of a first degree consanguineous
a. DOPA
couple was noted by the parents to have darkening of
b. Norepinephrine
the urine to an almost black colour when it was left
c. Serotonin
standing. He has normal siblings, and there are no
d. Thyroxine
other associated medical problems. Growth and
development to date are normal. Which of the
2. A child presents to OPD with complaints of frequent
following enzymes is likely deficient in this patient?
nausea and vomiting episodes. On detail history and
a. Homogentisate oxidase
examination, the child says that the episodes are
b. Phenylalanine hydroxylase
precipitated by consumption of meat, eggs and other
c. Tyrosinase
protein rich foods. The doctor prescribes
d. Fumarylacetoacetate hydroxylase
phenylbutyrate with dietary restriction for proteins.
The mode of action of phenylbutyrate in this patient
8. Amino acid lost in urine in cystinuria are all except:
is by :
a. Cystine
a. Decreasing nitrogen absorption
b. Cysteine
b. Providing extra ATP
c. Lysine
c. Removing Nitrogen by alternate route
d. Argine
d. Increasing gut motility
9. Deficiency of which of the following amino acids can
3. A child presents with light sensitivity, eczema and hair
cause the condition shown in the figure:
loss. The urine has a characteristic odour. There is
history of epileptic episodes. Guthrie test is positive.
All of the following are true except:
a. Due to deficiency of enzyme phenylalanine
hydroxylase
b. White patch of hair due to tryptophan deficiency
c. Phenylacetate present in urine
d. Mental retardation is present

4. In phenylketonuria the main aim for first line therapy

is
a. To replace the deficient product
b. To reduce the substrate for the enzyme
c. Change in confirmation of the enzyme a. Tryptophan
d. Supplementation of the limiting AA b. Phenylalanine
c. Cysteine
5. An infant presents with with hepatomegaly, d. Tyrosine
jaundice,and marked edema. There is a distinctive
cabbag-like odor in urine. Mother gives history of 10. Which of the following urine odours is not correctly
poor weight gain since birth. Tyrosine and methionine matched:
are elevated in blood. The enzyme deficiency is likely a. Sweaty feet odour: isovaleric academia
to be: b. Mousy/musty odour: Phenylketonuria
a. PHPP dehydrogenase c. Burnt sugar odour: MSUD
b. Tyrosine ligase d. Swimming pool odour: Tyrosinemia
c. Fumarylacetoacetate hydrolase
d. Tyrosine transminase 11. Glutathione does all of the following except?
a. Participates in the transport of amino acids across
6. Substrate linking Krebs’s cycle with urea cycle is: some cell membranes
a. Aspartate b. Scavenge peroxides
b. Fumarate c. Decreases the stability of erythrocyte membranes
c. Alanine d. Form conjugates with some drugs to increase
d. Arginine water solubility

12. Auxiliary proteins assisting in folding include allexcept:

a. Heat shock proteins
b. Chaperonins
c. Ubiquitin
d. Calnexin
13. A previously diagnosed hepatitis C patient presents a. Waldenstrom macroglobulinemia
with end-stage liver disease. he has developed b. Rheumatoid arthritis
profound muscle wasting, marked ascites, and severe c. Non-Hodgkin lymphoma
hepatic encephalopathy. Mild jaundice is present. d. Sickle cell anemia
Which of the following are likely to be present in
normal amounts? 21. The picture shows the separation of a mixture of
a. Albumin proteins by a combination of techniques. This process
b. Immunoglobulins is called two dimensional electrophoresis. The
c. Plasma Enzymes techniques used for obtaining this result are:
d. Acute Phase Proteins

14. True about protein structure is?

a. Secondary structure is decided by amino acid
sequence
b. Tertiary structure is responsible for stability.
c. Sulphide bond is present in secondary structure of
protein
d. Primary structure is lost on heating/denaturation

15. Which of the following glycoprotein has both

lubricant and protective action?
a. Immunoglobulin
a. SDS PAGE and HPLC
b. Mucin
b. HPLC and IEF
c. Collagen
c. IEF and SDS PAGE
d. Albumin
d. Native PAGE and IEF
16. Which of the following is a conformational disorder? 22. A girl with habit of eating paint peeled off from the
a. Prion Disease wall presented with complaints of acute abdominal
b. Osteogenesis imperfect
pain on and off with tingling sensation of limbs
c. Wilson’s disease
associated with weakness of the limbs. Which of the
d. Goodpasture’s syndrome
enzyme will show reduced activity in this patient?
a. ALA synthase
17. Type of collagen found in wound at day 5 of healing?
b. ALA dehydratase
a. Type I
c. Uroporphyrinogen III synthase
b. Type II
d. Coproporphyrinogen oxidase
c. Type III
d. Type IV
23. Which of the following is not a finding of acute
intermittent porphyria?
18. Menke’s disease is due to defect of which enzyme?
a. Lysyl oxidase a. Abdominal Pain
b. Photosensitivity
b. Lysyl Hydroxylase
c. Neuropsychiatric complaints
c. Prolyl oxidase
d. Positive Ehrlich’s Test
d. Prolyl Hydroxylase
19. Defective protein in Marfan’s syndrome is:
24. Which of the following enzymes does not contain
a. Collagen
Zinc?
b. Fibrillin
a. Alcohol dehydrogenase
c. Elastin
b. Carbonic anhydrase
d. Ankyrin
c. ALA Dehydratase
d. Aconitase
20. Monoclonal pattern serum protein electrophoresis,
asseen below, is seen in:
25. Which of the following graph is of Gamma chain
Haemoglobin?
 31. DNA Methylation is not related with?
a. Mismatch repair
b. DNA Replication
A A c. Gene silencing
B d. Capping

32. CpG islands show:

a. Methylation
b. Demethylation
c. Acetylation
B d. Phosphorylation
D C
33. Klenow fragment is due to absence of which
enzymatic activity?
a. A a. 5’-3’ polymerase activity
b. B b. 5’-3’ exonuclease activity
c. C c. 3’-5’ polymerase activity
d. D d. 3’-5’ exonuclease activity

26. A newborn is found to have mild jaundice at delivery.
Mother is Rh negative. There is history of prior
delivery at home for a previous pregnancy, the older
sibling is Rh positive. Which of the following will be
incorrect in this patient?
a. Raised unconjugated bilirubin
b. Raised stool uroblilinogen
c. Raised urine urobilinogen
d. Increase urine bilirubin
27. Heme breakdown produces all except:
a. Ferric ion
b. CO
c. CO2
d. Biliverdin
28. A patient presents to the OPD with well developed jaundice
along with other symptoms. On detailed examination and
investigations, the patient is provisionally diagnosed to be
suffering from carcinoma of head of pancreas. Which of
the following is not true for this patient?
34. Hayflick limit is?
a. Total no. of times a cell can divide in its lifetime in
vivo
b. Total number of progenies a stem cell can make
c. Total number of times a cell can divide in cell
culture
d. Total number of times a cancer cell can divide
35. Which of the following is not a constitutive
heterochromatin?
a. Satellite DNA
b. Barr Body
c. Centromere
d. Telomere
36. In SCID, what type of DNA repair is defective?
a. Single strand DNA break repair
b. Nucleotide excision repair
c. Mismatch repair
d. Non-Homologous End Joining
37. About mt.DNA, all are true except:
a. It is double stranded and circular
b. A total of 37 genes are encoded by the mt DNA
c. All proteins of ETC come from mt DNA genes
d. Introns are not present in mt DNA

38. A 19 year old male patient presents to emergency with

apahsia, cortical blindness and hemiparesis. There is
a. Decreased urobilinogen in urine history of migraine like headaches and seizures. The
b. Decreased bilirubin in urine patient's friend also gives hsitory of exrcise
c. Dark yellow (mustard coloured) urine intolernace. The maternal uncle of the patient had
d. Pale (clay coloured) stool similar history. Based on the detailed family history,
the following pedigree chart was created. What type of
29. MRP-2 deficiency is seen in which disorder? inheritance pattern is depicted in the pedigree chart?
a. Dubin Johnson Syndrome
b. Criggler Najjar Syndrome-I
c. Criggler Najjar Syndrome-II
d. Gilbert Syndrome

30. Severe jaundice is seen in?

a. Dubin Johnson Syndrome
b. Criggler Najjar Syndrome-I
c. Criggler Najjar Syndrome-II
d. Gilbert Syndrome
 45. Which enzyme has proof reading function in PCR?
a. Taq polymerase
b. Pfu polymerase
c. T-7 polymerase
d. Bst polymerase

46. Northern blot used for

a. DNA
b. RNA
c. Protein
d. DNA protein interaction

47. Name the phenomenon:

a. Autosomal Dominant
b. Mitochondrial inheritance
c. Autosomal Recessive
d. X-Linked recessive

39. In which RNA, 5’ end is uncapped?

a. mRNA
b. snRNA
c. pri-mi-RNA
d. tRNA

40. Attachment at 3’ end of tRNA after post-

transcriptional modification is: a. Transformation
a. Poly A tail b. α- Complementation
b. 7-methylguanosine c. Yeast Double hybrid
c. 5- methylguanosine d. Translation
d. CCA
48. In the following figure of agarose gel
41. All are ribozyme, except:
a. 23S rRNA electrophoresis identify the length of the marked
b. 28S rRNA PCR amplified DNA fragment
c. Peptidyl transferase
d. 18S rRNA
42. Assertion: According to central dogma
DNA………>RNA ................ >Proteins
Reason: Reverse of central dogma in retro virus ie
Reverse Transcriptase
convert
RNA………>DNA………..>RNA >Proteins
a. Assertion is True, Reason is True, Reason is
thecorrect explanation for Assertion
b. Assertion is True, Reason is True, Reason is not
thecorrect explanation for Assertion
c. Assertion is True, Reason is False a. 480bp
d. Assertion is False, Reason is False b. 312bp
c. 215bp
43. Which does not happen from 5’ to 3’ direction? d. Cannot be predicted
a. DNA Replication
b. DNA Repair 49. Single Nucleotide Polymorphism is due to:
c. RNA Editing a. Insertion
d. Transcription b. Deletion
c. Inversion
44. Translocation of ribosome is carried out by: d. Point Mutation
a. EF-2
b. eIF-4E 50. Restriction Endonuclease will cut at:
c. eIF-1,3 a. AAGCTT
d. RF-3 b. AGAGAG
c. AAGAAG
d. AGTTG