XN-L Series PM Standard Procedure

Sysmex Corporation
Customer Support Headquarters

First edition December 01, 2014

 (First Edition)
XN-L Series Preventive Maintenance Checklist

Items to be checked Schedule Check Remarks

Check data
Check data using control
before ☐
blood plasma
maintenance
Transducer aperture cleaned Once a year ☐
RBC Detector Draining isolation chamber Once a year ☐
Block
Clean inside. Once a year ☐

RBC/HGB Cleaning chamber Once a year ☐

Chamber Clean Filter No. 13 Once a year ☐
WBC chamber Cleaning chamber Once a year ☐
and RET
chamber Clean Filter No. 13 Once a year ☐
Clean Waste chamber Once a year ☐
Rinse Trap Chamber Once a year ☐
Waste Chamber
Replace pinch valve Every 3 years or 60,000
□
Pharmed Tube (3 pcs) cycles
Clean Flowcell Once a year ☐
FCM Unit
Check laser current value Once a year ☐
Clean Vacuum Line Once a year ☐
Vacuum Line
Rinse Trap Chamber Once a year ☐
Clean pipette/piercer Once a year ☐
pipette or probe Piercer Replacement Every 120,000 cycles ☐
(XN-350)
Piercer Clean Rinse Cup Once a year ☐
(XN-450/XN-550)
Clean protecter Once a year ☐
Sheath syringe Check for leakage Once a year ☐
WB pump Check for leakage Once a year ☐
Sampler (XN- Lubricate the drive unit. Once a year ☐
550) Clean inside. Once a year ☐
Check 0.06 MPa Once a year ☐
Pneumatic Check -0.03 MPa Once a year ☐
system Every 2 years or 30,000
Replace air pump ☐
cycles
Check/Adjustment
Once a year ☐
pipette/piercer position
Mechanical
Check/Adjustment sampler
Adjustment
position Once a year ☐
(XN-550)
Adjustment blood aspiration
Once a year ☐
sensors
Electronics
Adjusting RBC Clog Level Once a year ☐
Adjustment
Adjust HGB background count
Once a year ☐
level
Adjustment Sensitivity adjustment Once a year ☐
sensitivity/data Check data calibration Once a year ☐
Inside and Clean Main Unit covers Once a year ☐
outside of Main
Unit Clean inside Once a year ☐
Clean IPU Touch Panel Once a year ☐

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 (First Edition)

Items to be checked Schedule Check Remarks

Cleaning peripheral device

Once a year ☐
(printer, etc.)

Run Scandisk Once a year ☐

IPU
Check program version Once a year ☐
ID Read Test ☐

Operation Check Host Communication Test ☐

Check data using control
☐
material or samples
Reset Cycle Counter of
Check after maintenance ☐
maintenance Reset
Final performance check ☐
“Schedule” is only a guideline and differs according to status of use.
If “Schedule” is blank, it means that the items need to be checked at every periodic maintenance.
Replaced part which is out of PM, parts for recommended in future are described in remarks box.

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【Details of Adjustment Items】
Adjustment Items Specified Range Value Before Adjustment Value After Adjustment
CLOG LEVEL 100 +/- 1
ADJUSTMENT
HGB background convert 5000 +/- 200
blood aspiration sensor 5000 +/- 200
blank
Blood Asp sensor
13000 +/- 500
Sample - blank
Sensitivity Adjustment XN-CAL Target Value (Lot No.　　　　　　　)
WDF-X Target value +/-3ch
WDF-Y Target value +/-3ch
WDF-FSC Target value +/-2h
RET-RBC-X Target value +/-3ch
RET-RBC-Y Target value +/-3ch
RET-RBC-Z Target value +/-2ch
MCV Target value +/-1fL
MPV Target value +/-3fL
HGB Target value +/- 0.2g/dL

【Details of Check Items】

Adjustment Items Specified Range Checked value
Laser Current 120 +/- 20mA
Program version -
* Refer to Job List or QC Chart for control, or sample analysis values.

Maintenance
Report No.
Facility Name
Instrument Name XN-350／XN-450／XN-550 Serial No.
Maintenance
Date
Name of Service
Engineer

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 (First Edition)
XN-L Series PM Standard Procedure
[Required jigs and controls, etc.]
Code Name Remarks
- XN CHECK Control blood
- XN CHECK BF Control blood for BF
- XN CAL Calibration by Calibrator

Operatio
Items to be checked Procedure
n time

Check data Analyze normal sample or control material

Check data using control blood
before 5 min. and print average values or record them
plasma
maintenance using stored data/QC chart.
Apply CELLCLEAN to a brush for clog
Transducer aperture cleaned
removal and clean the aperture.
RBC Detector Perform draining sequence for RBC isolation
Draining isolation chamber 10 min.
Block chamber
Clean inside. Remove dust, etc. from inside of Main Unit.
Drain chamber by selecting Maintenance -
Drain Waste Fluid Chamber. Clean waste
Cleaning chamber 6 min. line and chamber by pouring CELLCLEAN
RBC/HGB
and perform "Drain Waste Fluid Chamber"
chamber
again.
WBC Chamber
RET Chamber Remove Filter No. 13 in waste line from
reaction chamber clean it using syringe with
Clean Filter No. 13 10 min.
CELLCLEAN. Make sure that push the
syringe in reverse direction.
Clean using CELLCLEAN and distilled
water.
5 - 15
Clean Waste chamber If necessary, clean waste chamber by
min.
selecting Maintenance Drain Waste Fluid
Waste Chamber Chamber.
Clean using CELLCLEAN and distilled
Rinse Trap Chamber 5 min.
water.
Replace pinch valve Pharmed
10 min. Replace with TUBE PHARMED 5X3 (50mm).
Tube (3 pcs)
Disconnect tube at nipple No. on WC2 and
front tube on SV 12. Dip one end of the tube
into a cup with CELLCLEAN. Connect a
Clean Flowcell 5 min.
syringe to the other end of the tube and
FCM Unit move piston more than 20 times to clean the
inside of flow cell.
Check laser current value by selecting
Check laser current value 5 min.
Maintenance - Sensor.

Disconnect vacuum line tube at the nipple

No. 7 on WC1 and aspirate methanol to
Clean Vacuum Line
clean vacuum line. Discard the methanol in
Vacuum Line 5 min.
the trap chamber.
Clean using CELLCLEAN and distilled
Rinse Trap Chamber
water.
Clean pipette outer with gauze moistened
Clean pipette/piercer
with CELLCLEAN.
pipette Replace piercer with ASP ASSY (PM)
(XN-350) Piercer Replacement
Piercer
5 min. No.8.
(XN-450/XN-550) Clean using CELLCLEAN and distilled
Clean Rinse Cup
water.
Clean using CELLCLEAN and distilled
Clean protecter
water.
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Operatio
Items to be checked Procedure
n time
Check for leakage at syringe clean it if
Sheath syringe Check for leakage 5 min. necessary. Clean the motor shaft and
lubricate it.
Check for leakage at syringe clean it if
WB pump Check for leakage 5 min.
necessary.

Lubricate the drive unit. Lubricate rack related mechanism.

Sampler (XN-
5 min.
550) Remove instrument covers and remove dust,
Clean inside.
etc. in the instrument.
Check the 0.06MPa pressure by selecting
Check 0.06 MPa
Maintenance - Sensor.
Pneumatic 5 min.
Check the 0.03MPa pressure by selecting
system Check -0.03 MPa
Maintenance - Sensor.
Replace air pump 10 min. Replace with Air Pump Set No. 1.
Check/Adjustment pipette/piercer Check the piercer position. Adjust it if
5 min.
position necessary.
Mechanical
Check/Adjustment sampler
Adjustment Check the positions for Sampler, Hand and
position 5 min.
Bar code reading. Adjust them if necessary.
(XN-550)
Adjustment blood aspiration Check the blood aspiration sensor. Adjust it if
sensors necessary.
Electronics Check the RBC clog sensor. Adjust it if
Adjusting RBC Clog Level 5 min.
Adjustment necessary.
Adjust HGB background count Check the HGB blank level. Adjust it if
level necessary.
Check the sensitivity using XN CAL. Adjust it
Sensitivity adjustment
Adjustment if necessary.
20 min.
sensitivity/data Check the sensitivity using XN CAL. Adjust it
Check data calibration
if necessary.
Clean Main Unit covers Clean main unit cover.
Clean inside Clean inside of the main unit.
Inside and
outside of Main 5 min. Wipe the touch panel using gauze moistened
Clean IPU Touch Panel
Unit with ethanol.
Cleaning peripheral device
Clean peripheral devices.
(printer, etc.)
Run Scandisk Run Scandisk
IPU 5 min. Check the program version by selecting
Check program version
“Version" in toolbar.
Check whether ID is read correctly by
ID Read Test
analyzing samples.
Check whether host computer
Host Communication Test communication performed correctly by
Operation Check 10 min.
analyzing samples.
Check whether the values are the same
Check data using control material
before and after the work with samples or
or samples
control blood.
Reset the cycle count of the replaced parts
Reset Cycle Counter of
by selecting Maintenance - Status
maintenance
Confirmation - Counter after entering Service
Reset
Check after Mode.
10 min.
maintenance Turn ON the power and check that the
instrument is operating without errors during
Final performance check
sample analysis.
Cofirm that IPU or main unit is normal mode.
*　Operation time is an indication and does not include removal of covers, etc.

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 (First Edition)
XN-L Series Periodic Replacement Parts List

Time for Replacement (Year)

Part No. Part Name 1. 1. 2. 3. 4. 5. 5.
0.5 2.0 3.0 4.0 6.0
0 5 5 5 5 0 5
Ye Ye
TUBE PHARMED_BPT_3 X s s
442-6485-1
5
Every 60,000 cycles
Ye Ye Ye
s s s
051-0471-1 AIR PUMP SET NO.1 (PM)
Every 30,000 cycles
AN965961 ASP_ASSY(PM) No. 8 Every 120,000 cycles
“Time for Replacement” is only a guideline and differs according to status of use.

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 (First Edition)
Revision History

Edition Number Date of Revision Revision Details

First edition December 1, 2014 This is the first edition.