DNAmakes upthe genome Packauged

into chromosomes carry several 100 woo's of genes

s
Eukaryote prokaryote
severalmolecules
1 molecule

1 interphase G Phase SPhase s G Phase

Cellgrows4expands Cellcontinuesgrowing Cellcontinues
90 ofcellcycle DNA duplicates
growing
forcelldivision
Preparation

2 M phase mitosis
prophase PROMetaphase Metaphase Anaphase

Nucleolus disappeared NuclearEnvelopebegins Nuclearenvelope

gone Chromatids
pull apart
Chromatinscondenseinto disappearing Chromatidsline into daughter chromosomes
upalong
of2chromatids
chromosomesconsisting Asters
movetoopposite metaphaseplate Cell stretches out
Asterpairof centrosomes move ends of cell expandspindle

partand formearlymitoticspindle Presence

of
kinetochore attached
lolcentromere
kinetochoremicrowbute
fiber attached
span
to 1centromere
Wonka MtFibernocentromere

Telophase cytokinesis
Nucleolus 3 WEbegin terming
Spindle fibers break apart

Cleavage furrow First sign of cleavage cytokinesis process

a shallow groove near the old metaphase plate

cell cycle control system

Most imp G if itdoesntpassitwillgoto Go nondividing
Mcheckpoint Occursat prometaphase and willnot continue
if anychromosome is not attached
Gzcheckpoint between metaphase and anaphase doesn
continue until all chromosomes areattached to a spindle fiber
 cyclins calks
acdks must be attached to a cyclin to be active
MPF maturation promotingfactor is a cyclincdk complex that triggers a
cell to move past the Gz checkpoint
Acb as a proteinkinase

cyclin 3 Mpf peaks correspond

cyclin is degraded after Mphaseand replaced
with Cdk while cyclin accumulates untilthe Gz M
checkpoint where it binds to cdk toform Mpfwhich
is only active in M phase