Protocol report

Presto_MVP_200uL_VX2_Mmtm_V2_1,,,
2022-06-27 13:32:54+08:00 1/6

General info

Protocol information
Protocol name Presto_MVP_200uL_VX2_Mmtm_V2_1,,,
Modified by hannah.pezzi
Kit name MagMAX MVPI and MVP II
Description MagMAX MVP for Viral RNA extraction from 400uL Liquid sample

Run information
Execution started 2022-06-27 13:13:53+08:00
Executing user ameer.affandi
Total warnings 0
Total errors 0

Laboratory information
Laboratory name
Address
Phone
E-Mail

Instrument parameters
Instrument name 713-8G0101
Instrument type KingFisher Presto
Firmware version 1.2.3
Instrument serial number 713-8G0101

Software parameters
Software version BindIt Software 4.0 for KingFisher Instruments

Run log
2022-06-27 13:13:53 Presto_MVP_200uL_VX2_Mmtm_V2_1,,,: started
2022-06-27 13:13:53 Temperature: 28 / 33 °C
2022-06-27 13:13:56 Pick-Up: 'Tip1' load.
2022-06-27 13:13:56 Load plate: started
2022-06-27 13:14:07 Load plate: started
2022-06-27 13:14:20 RotateDial: started
2022-06-27 13:14:22 Change plate: started
2022-06-27 13:14:22 Remove plate: started
2022-06-27 13:14:22 Load plate: started
2022-06-27 13:14:22 Extraction0: started
2022-06-27 13:14:22 Change plate: started
2022-06-27 13:14:22 Remove plate: started
2022-06-27 13:14:22 Load plate: started
2022-06-27 13:14:23 Temperature: 31 / 33 °C
2022-06-27 13:14:25 Change plate: started
2022-06-27 13:14:25 Remove plate: started
2022-06-27 13:14:25 Load plate: started
2022-06-27 13:14:25 Extraction: started
Protocol report
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2022-06-27 13:14:25 Change plate: started

2022-06-27 13:14:25 Remove plate: started
2022-06-27 13:14:25 Load plate: started
2022-06-27 13:14:53 Temperature: 40 / 34 °C
2022-06-27 13:15:23 Temperature: 48 / 35 °C
2022-06-27 13:15:53 Temperature: 55 / 35 °C
2022-06-27 13:16:23 Temperature: 62 / 36 °C
2022-06-27 13:16:53 Temperature: 68 / 37 °C
2022-06-27 13:17:23 Temperature: 69 / 37 °C
2022-06-27 13:17:53 Temperature: 69 / 36 °C
2022-06-27 13:18:23 Temperature: 70 / 36 °C
2022-06-27 13:18:53 Temperature: 70 / 36 °C
2022-06-27 13:19:23 Temperature: 70 / 36 °C
2022-06-27 13:19:53 Temperature: 70 / 35 °C
2022-06-27 13:20:23 Temperature: 66 / 35 °C
2022-06-27 13:20:53 Temperature: 63 / 34 °C
2022-06-27 13:21:04 Wash1: started
2022-06-27 13:21:06 Change plate: started
2022-06-27 13:21:06 Remove plate: started
2022-06-27 13:21:06 Load plate: started
2022-06-27 13:21:23 Temperature: 61 / 34 °C
2022-06-27 13:21:53 Temperature: 67 / 34 °C
2022-06-27 13:22:23 Temperature: 70 / 35 °C
2022-06-27 13:22:53 Temperature: 70 / 35 °C
2022-06-27 13:23:23 Temperature: 70 / 35 °C
2022-06-27 13:23:41 Wash2: started
2022-06-27 13:23:43 Change plate: started
2022-06-27 13:23:43 Remove plate: started
2022-06-27 13:23:43 Load plate: started
2022-06-27 13:23:53 Temperature: 70 / 35 °C
2022-06-27 13:24:23 Temperature: 70 / 35 °C
2022-06-27 13:24:24 Elution1: started
2022-06-27 13:24:24 Change plate: started
2022-06-27 13:24:24 Remove plate: started
2022-06-27 13:24:24 Load plate: started
2022-06-27 13:24:53 Temperature: 70 / 35 °C
2022-06-27 13:25:00 Change plate: started
2022-06-27 13:25:00 Remove plate: started
2022-06-27 13:25:00 Load plate: started
2022-06-27 13:25:23 Temperature: 70 / 35 °C
2022-06-27 13:25:53 Temperature: 69 / 35 °C
2022-06-27 13:26:23 Temperature: 69 / 35 °C
2022-06-27 13:26:53 Temperature: 70 / 35 °C
2022-06-27 13:27:23 Temperature: 70 / 35 °C
2022-06-27 13:27:53 Temperature: 70 / 35 °C
2022-06-27 13:28:23 Temperature: 70 / 35 °C
2022-06-27 13:28:53 Temperature: 70 / 35 °C
2022-06-27 13:29:23 Temperature: 70 / 35 °C
2022-06-27 13:29:53 Temperature: 70 / 35 °C
2022-06-27 13:30:21 Elution2: started
2022-06-27 13:30:23 Temperature: 70 / 35 °C
2022-06-27 13:30:53 Temperature: 67 / 34 °C
2022-06-27 13:31:23 Temperature: 64 / 34 °C
2022-06-27 13:31:53 Temperature: 61 / 33 °C
2022-06-27 13:32:23 Temperature: 58 / 33 °C
2022-06-27 13:32:31 Leave: 'Tip1' leave.
Protocol report
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2022-06-27 13:32:33 Remove plate: started

2022-06-27 13:32:47 Remove plate: started
2022-06-27 13:32:53 Temperature: 55 / 33 °C
2022-06-27 13:32:53 Presto_MVP_200uL_VX2_Mmtm_V2_1,,,: completed
Protocol report
Presto_MVP_200uL_VX2_Mmtm_V2_1,,,
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Reagent info

Sample 96 DW plate
Name Well volume [µl] Total reagent volume [µl] Type
Binding/Bead Mix 275 - Reagent
Sample 200 - Reagent
PK/MS2 10 - Reagent

Wash 96 DW plate
Name Well volume [µl] Total reagent volume [µl] Type
Wash Soln 500 - Reagent

80p_EtOH 96 DW plate
Name Well volume [µl] Total reagent volume [µl] Type
80% Ethanol 1000 - Reagent

Elution 96 DW plate
Name Well volume [µl] Total reagent volume [µl] Type
Elution Solution 100 - Reagent

TipComb 96 DW plate
Name Well volume [µl] Total reagent volume [µl] Type
- - - -

Empty 96 DW plate
Name Well volume [µl] Total reagent volume [µl] Type
NoReagent 50 - Reagent

Dispensed reagents

The protocol does not contain dispensed reagents

Protocol report
Presto_MVP_200uL_VX2_Mmtm_V2_1,,,
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Steps data

Tip1 96 DW tip comb

Pick-Up TipComb

RotateDial Empty
Beginning of step Precollect No
Release beads No
Mixing [none]
Heating during mixing No
End of step Postmix No
Collect beads No

Extraction0 Sample
Beginning of step Precollect No
Release beads No
Mixing [none]
Heating during mixing No
End of step Postmix No
Collect beads No

Extraction Sample
Beginning of step Precollect No
Release beads Yes
Mixing / heating: Mixing time, speed 00:05:00, Fast
Heating temperature [°C] 70
Preheat Yes
End of step Postmix No
Collect count 5
Collect time [s] 0

Wash1 Wash
Beginning of step Precollect No
Release time, speed 00:00:10, Bottom mix
Mixing / heating: Shake 1 time, speed 00:00:10, Bottom mix
Shake 2 time, speed 00:00:10, Fast
Loop count 2
Heating during mixing No
End of step Postmix No
Collect count 5
Collect time [s] 1

Wash2 80p_EtOH
Beginning of step Precollect No
Release beads No
Mixing / heating: Mixing time, speed 00:00:20, Medium
Heating during mixing No
End of step Postmix No
Collect beads No
Protocol report
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Elution1 Elution
Beginning of step Precollect No
Release beads Yes
Mixing / heating: Shake 1 time, speed 00:00:15, Bottom mix
Shake 2 time, speed 00:00:45, Medium
Loop count 5
Heating temperature [°C] 70
Preheat Yes
End of step Postmix No
Collect beads No

Elution2 Elution
Beginning of step Precollect Yes
Release beads No
Mixing / heating: Mixing time, speed 00:01:00, Slow
Heating during mixing No
End of step Postmix No
Collect count 2
Collect time [s] 15

Leave TipComb