Model: CS-T240Plus
Auto-Chemistry Analyzer Operation Procedure
This operation procedure is applicable to the Auto-Chemistry Analyzer,
hereinafter referred to as the Analyzer.
1 Power on
1.1 Check before power on
(1)Sample adding system: check whether the probe and the mixing bar are
covered with water droplets and dirt; whether it is bent or blocked; whether each
rinsing bath is dirty or blocked. If the above situation occurs, please refer to
“Auto-Chemistry Analyzer Maintenance Procedure” for maintenance.
(2)Detergent: Before the test, check the detergent on the left side of the Analyzer
and add it when it is insufficient.
Please use the original detergent.
(3)Waste liquid tank: Ensure that there is enough space in the waste liquid tank to
hold the waste. When the tank is full, please drain the waste and clean the waste
liquid tank immediately.
(4)Printer: Check whether the printer is properly installed and whether the paper
is sufficient.
(5)Analyzer Worktable: Check whether the Analyzer worktable is clean and free
of debris.
(6)Power supply: Check that the UPS power switch is in the ON state.
(7)Connection: The analyzer is correctly connected to the computer host with a
communication cable.
The new cuvettes should be immersed in 2% CS-Anti-Bacterial
Phosphor-Free Detergent for more than 8 hours before installation, then
rinsed repeatedly with tap water, then rinsed with pure water, dried in air,
and then installed on the reaction disk.
1.2 Power on
(1)Turn on the main power switch above the power socket on the right side of the
Analyzer.
In order to ensure the refrigerating effect of the sample reagent
compartment, when the reagent is stored, the main power switch should be
in the ON state.
(2)Turn on the power switch (analysis part power supply) next to the main power
switch on the right side of the Analyzer.
(3)Turn on the computer, enter the “Auto-Chemistry Analyzer” operating
software, the Analyzer enters the standby state before proceeding to conduct the
next step.
1.3 Reagent preparation after power on
To ensure accurate test results, please use the original reagents.
1.3.1 Colorimetric item
(1)Click “Reagent” on the main interface of the software to check the remaining
amount of each reagent.
(2)Reagent replacement: Replacing the reagents in time with the expected amount
of measurement on the day (reagents of different lot numbers cannot be mixed),
the reagent position is displayed on the screen, and attention should be paid to the
presence of air bubbles in the reagent bottle.
(3)Reagent remaining volume scan: Select the item to be scanned, click the
[Volume Scan] button, and the Analyzer automatically performs the remaining
reagent scan of the selected reagent position.
1.3.2 Preparation of ISE item
(1)Activate ISE
Before performing the ISE test, you must first select “Use ISE” in the “Other
Info” window in the “System Setting”. All settings on ISE in the software are
activated.
Only when the Analyzer is equipped with an ISE module and ISE is in
standby, “Use ISE” can be selected; otherwise this option is gray.
(2)Place reagent
Place the internal standard solution, diluent, and reference solution in the
electrolyte reagent rack on the left side of the analysis unit.
(3)Input the remaining amount of reagent
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In the “Item Parameters” window in “System Setting”, select the ion item and set
the ISE reagent volume. After the Analyzer uses ISE reagent, the remaining
amount is automatically decremented and displayed in the ISE reagent volume
setting workspace.
(4)In the “System Maintenance” window, execute rinse of all ISE pipelines.
2 Sample collection and pretreatment
The analyzer is mainly used for the clinical biochemical tests of blood (serum,
plasma), urine, cerebrospinal fluid, and pleural effusion. As the anticoagulant in
the plasma interferes with the test results, serum samples are mostly used for
clinical purpose. Accurate collection, preservation, and separation of samples are
an important part of clinical biochemical tests. Improper sample collection and
pretreatment may affect the accuracy of test results.
2.1 Sample collection
(1)Blood samples
Sample container: Biochemical vacuum blood collection tube.
Collection site: Elbow veins are often involved.
Collection volume: 3mL~5mL.
(2)Collection of other body fluids
Pleural fluid, ascites, cerebrospinal fluid, etc., should be collected using sterile
puncture, and the amount collected should not be less than 1mL.
2.2 Pretreatment of serum samples
(1)Preparation of serum sample: After blood coagulation, centrifuge at a speed of
3000R/min for 5 minutes to 10 minutes. In summer, the serum precipitates faster.
It is advisable to separate the serum about half an hour after the blood coagulation.
In winter, the clot shrinks slowly. The blood can also be placed in a 37℃ water
bath to promote the serum to precipitate. When the blood is left for too long, the
biochemical components are prone to change. If the time is too short, the serum is
not completely separated. The fibrin suspended in the serum will block the probe
and affect the normal operation of the test. (After centrifugation, make sure that
no fibrin filaments are floating in each sample, otherwise the probe will become
clogged).
(2)Preparation of body fluid samples: clear and transparent body fluid samples
can be directly loaded for use. Turbid or flocculent ones should be centrifuged
and then the supernatant should be taken. The supernatant should be free from
hemolysis, fibrin, clots, blood cells and flocculent suspensions, and the amount of
supernatant should not be less than 1 mL.
● Samples with severe hemolysis and a sample volume of less than 1 mL are
unqualified serum samples and must be collected and centrifuged again.
● Samples of bodily fluids that have been stored for more than 4 hours may
have been contaminated with bacteria, and they are unacceptable samples.
● Samples should be submitted immediately after collection.
● The amount of serum used for testing should be greater than 200μL.
3 General operating procedures
3.1 Calibration process
(1)Place the calibrator in the set position.
(2)Calibration of colorimetric item: After entering the “Calibration Info” window,
click the type of calibration you want to select in the “Calibration Info” window,
select the item to be calibrated, click [Register], and the calibration item will be
saved in the calibration list. In the calibration registration interface, set the
calibrator position and save it.
(3)ISE Calibration: After entering the “Calibration Info” window, on the “ISE
Calibration” interface, select the ISE item from the pull-down menu, enter the
concentration, disk number and position of the item, click [Save Settings], and
check “Execute ISE Calibration.”
The sample volume of ISE is a fixed value of 45 μL and does not require the
operator to set it.
(4)Click [Send Test], select “Calibration Test”, and click [Start Test] to execute
the calibration test.
Before the test, confirm whether the status of the item to be calibrated in the
calibration list is execution. During routine testing, the calibration test is
performed first.
REV. 09-2020

Auto-Chemistry Analyzer Operation Procedure
3.2 QC process
3.2.1 Prepare QC serum
Place the control serum in the set position.
3.2.2 QC test
(1)Register QC item: Enter the “QC Registration” interface in the “QC
Management” window. Enter or select the Cup type, QC name, QC validity
period, QC lot number, QC item, sample type, target value, and standard
deviation on the right side of the interface. Click [Add QC]. The QC item is saved
to the left QC item list. Click [Edit Position], select the registered QC item from
the list, set the place for placing the QC serum, click the [Update] button, and the
QC location is set successfully.
(2)QC Test: Click to select the “Execute” check box before the QC list, click
[Send Test], select “QC Test” on the “Start Test” interface, and click [Start Test]
to execute the QC test. After the test, check the QC results on the “Daily QC”
screen (Daily QC results can be accumulated and viewed in the monthly QC).
The QC test can be performed only when the QC item status is in execution.
During routine testing, QC tests are performed first.
3.3 Sample registration
3.3.1 Single sample registration
In the main interface, click the “Sample” option, and enter the following content:
(1)Sample number, disk number, and position number.
(2)Barcode ID number (You may not enter barcode).
(3)Sample types: serum, urine, plasma, cerebrospinal fluid, and pleural effusion.
(4)Sample cup types: test tubes, standard cups, and micro cups.
(5)Sample volume: normal volume, decrement, and increment.
(6)Dilution: Dilution.
(7)Select a test item or a combination of items.
After entering the above contents, click [Register] to register.
(8)Registering patient info: After registering the sample info, register the sample
number, medical record number, name, gender, age, patient type, test sending
department, test sending doctor, nationality, examiner, inpatient area, ward, bed
number, test sending date, etc. on the “Patient Info” interface. After registering,
click [Register], the sample number will be automatically incremented to the next
number, and repeat the above operation.
3.3.2 Batch sample registration
Perform batch registration if multiple sample test items are the same:
In the “Sample Registration” interface, according to the content of the “3.3.1
Single sample registration” section, register the initial sample info of this batch,
and click [Batch Register] to enter the end sample number or the batch sample
number, and click [Register] to complete the batch sample registration.
When conducting routine sample batch registration, you must first register
sample info and then register patient info.
3.4 Routine sample test
(1)According to the registered position, place the sample in the appropriate
position on the sample reagent disk.
(2)Click [Send Test], select “Routine” on the “Start Test” screen, enter the
starting sample number and ending sample number, and click [Start Test].
3.5 Emergency sample test
(1)Emergency sample registration: Select “Emergency”, register according to
steps (1) to (6) in 3.3.1, and click [Register]. Other operation is the same as that of
routine sample registration.
(2)After registration is completed, click [Send Test], select “Emergency” on the
“Start Test” screen, enter the starting sample number and ending sample number,
and then click [Start Test]. The emergency sample will be tested first.
3.6 Add samples
According to your needs, choose one of the following adding methods:
(1)Add a new sample: During the testing process, register a new sample (sample
info, patient info) on the “Sample Registration” screen, either by routine or
emergency sample registration. After registration, click [Send Test] again. After
selecting “Routine” or “Emergency” on the “Start Test” screen, enter the starting
sample number and ending sample number, and then click [Start Test] to send the
test adding command.
(2)Add other test items to the samples that have been tested: Click [Add Item] in
the “Result Query” window of the “Test Result” window, and select the sample
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Model: CS-T240Plus
number and analysis item to be added from “Analysis Items” and click [Add] to
save adding info; or input the sample number in “Manual Items”, select the item
info, and click [Add] to save the adding info. After registration, click [Send Test],
enter the starting sample number and ending sample number, and then click [Start
Test] to send the test adding command.
As for the addition of the manual item results, you must first register the
manual item info in the “Item Maintenance” window of the “System Setting”
screen; then you can add them to test results.
3.7 Recheck samples
(1)Automatic registration of recheck samples
On the “Item Retest” window of the “Test Result” interface, click [Condition
Setting] to set the recheck conditions. After the sample test is completed, the
Analyzer automatically registers the sample satisfying the conditions in the
recheck list and tests according to the set recheck conditions.
(2)Manual registration of recheck samples
After the sample test is completed, if there is a sample that needs to be rechecked,
select “Recheck” before the corresponding item on the test result screen. This
item is registered in the recheck list. Under the “Item Retest” interface, you can
select the dilution, increment, and decrement tests for the recheck item. After
confirming, click [Send Test], select “Rerun Test”, and click [Start Test].
3.8 Confirm and print results
(1)Results audit: After confirming the results on the “Result Query” interface of
“Test Result”, click [Audit], enter the sample number to be audited in “Sample
No. Range” and click the “Audit” button. Then the audit of the sample results can
be completed.
The sample of the audit must have the patient’s name and result. The sample
that has been audited cannot be deleted. Only the administration authority
can modify the result of the audit sample.
(2)Result printing: After confirming the result, click [Print] and enter the sample
number to be printed in “Sample No. Range” to print the sample result.
If a result is to be rechecked, the rechecked results must be audited to
cover the initial test results. If not, the initial test results will be printed.
4 Shutdown procedure
(1)After the test is completed on a daily basis, click [System Offline] on the main
screen, and then click [Log out], turn off the computer host and the monitor.
(2)Turn off the Analyzer’s power supply.
(3)Turn off the printer.
5 Precautions during testing
(1)The detergent should be regarded as a corrosive liquid. Once in contact with
your skin or eyes, rinse with plenty of water.
(2)Some items, due to the reagent formulation, may affect the results of analysis
of other items in the analysis process. The degree of contamination varies
according to the reagent. For details, contact the manufacturer or distributor of the
reagent. It is recommended that operators set up cross-contamination reagents
separately. If they cannot be completely separated, cross-contamination between
items can be reduced by using the cross-contamination avoidance function.
(3)When the Analyzer reads the barcode, it must perform a reset operation first,
and the probe mechanism, mixing mechanism, etc. will be started. Therefore, the
cover of the sample reagent disk should be closed. Do not reach into the motion
range of the moving parts of the Analyzer. Doing so may cause injury.
(4)Samples of hemolysis, lipemia, and jaundice will affect the test results.
(5)It should be ensured that the sample does not contain clots and is not turbid.
Otherwise, the probe will be blocked, which will seriously affect the test results.
(6)Some substances in the sample, such as drugs, anticoagulants, preservatives,
etc., may interfere with certain test results.
(7)Don’t leave the sample open for a long time, otherwise the sample will
volatilize and affect the test result.
(8)Failure to perform “system maintenance” according to the User Manual may
cause the Analyzer to become dirty or damaged and affect the test results.
(9)If system sleep is performed, it is not necessary to turn off the power of the
Analyzer and the power of the host computer or the monitor.