PLANT SCIENCE

(AGR122)

LAB 3 : STUDY OF POLLEN GERMINATION

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Prepared by;

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rs e Beverly Angel Boris (2020285568)
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Group;
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AT1101A3
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Lecturer;
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Ts. Rozlianah Fitri Said

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Date of submission;
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24 December 2020

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 INTRODUCTION :
To study pollen germination on a slide first we need to understand what the meaning of
pollination is and how it germinates. It is an essential part of a flowering plant’s life cycle.
Pollination is a process where pollen grains are transferred from the anthers to the stigma of
the same or different flowers unlike the study of pollen germination on slide. This process is
interceded by biotic and abiotic means. In biotic means, the pollination is carried out by a
mediator such as animals, birds, and insects. Abiotic means the pollen is transferred through
a non-living substance such as wind or water and not an organism. When we study pollen
germination on a slide the observation differs.

OBJECTIVES :
To study pollen germination on a slide.

MATERIALS & APPARATUS :

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a) Fresh mature flower

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b) Glass slide

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c) Glass rod rs e
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d) Dropper
e) Compound microscope
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f) 10 mg boric acid
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g) 30 mg magnesium sulphate
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h) 20 mg potassium nitrate
i) 10 g sucrose
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j) 100 ml of distilled water

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PROCEDURES :
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1. The pollen germination medium should be prepared by dissolving 10 grams sucrose, 10

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milligrams boric acid, 30 milligrams magnesium sulphate and 20 milligrams potassium nitrate
in 100 ml of distilled water.
2. The solution should be stirred by using a glass rod.
3. Take some nutrient solution by using a dropper and put two drops on a clean glass slide.
4. Take a mature flower and a few pollen grains should be dusted from its stamen on to the
drop on the slide.
5. Place the grass slide on the stage of the compound microscope after 5 minutes.
6. The slide should be observed through the microscope regularly for about half an hour.

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 RESULTS :

MAGNIFIC DRAWING DESCRIPTION

ATION
After an hour, the pollen grain is
uninucleate (has one nucleus)
in the beginning.

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In the first 10 minutes of

liberation, it becomes 2 celled,

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with a small generative cell and

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a vegetative cell.
400X

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Another 10 minutes, in the

nutrient medium the pollen
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grain germinates. The tube cell

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enlarges and comes out of the

pollen grain through one of the
400X germ pores to form a pollen
tube.
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At the end of 10 minutes, the

tube nucleus descends to the
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tip of the pollen tube. The

generative cell also passes into
it and divides into 2 male
400X gametes.

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 DISCUSSIONS :
Even during the experiment to study pollen germination on a slide, you will observe that
some pollen grains fail to germinate. Sometimes the nutrient mixture fails to do the job and in
real life when pollen doesn’t germinate, it’s because sometimes when the grain falls on the
stigma it does not get the right conditions to carry out germination. To study pollen
germination on a slide correctly remember, all pollen tubes don’t have the same length and
the newly developed ones have smaller tubes, unlike the old ones which are longer in size.
The pollen fly to stigma then the tube grows downwards.

CONCLUSION :
In this experiment, to study pollen germination on a slide the germinated pollen grains are
viable. When we study pollen germination on a slide we need to keep these things in mind,
first do not use dirty slides for dusting pollens, make sure it dry and clean. Next, drop only a
few drops of the nutrient median (2 – 3 drops). Finally, the observation is carried out on a
cavity slide that has a depression at the centre and use freshly plucked flowers only.

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