Name: Ira Monique H.

Cabaden Date Performed:  March 22, 2021

Year and Course: 1st year, BSN Date Submitted:  March 23, 2021
Subject title: Microbiology and Parasitology Code number: 9232
Name of Professor: Ms. Emelie Grace E. Cachero Score: ______________________

ACTIVITY 1
Safety in the Laboratory: General Rules inside the Laboratory

I. INTRODUCTION

In addition to safety risks associated with any clinical laboratory, such as chemical, fire,
electrical and radioactive hazard, the microbiology laboratory presents the hazard of exposure
to infectious agents, or biohazards. Biohazards are biological substances that may present a
health risk to humans. Guidelines to ensure safety in the clinical laboratory have been compiled
by several agencies. The recommendation of those agencies can be used to develop safety
regulations for the student microbiology laboratory.

II. OBJECTIVES

1. Determined the different safety guidelines for Clinical Laboratory.

2. Justify the differences of the 4 Biosafety levels.
III. ACTIVITY

a) On a bond paper make an illustration of the Safety Guidelines for the Clinical
Laboratory. Your illustration should be clear, precise and can be understood easily.
Kindly attach it here. (For your guide, refer to the given PDF)
 b. Complete the table:

BIOSAFETY BSL-1 BSL-2 BSL-3 BSL-4

LEVEL

Description Is considered the This level includes This level is considered The highest level of
lowest level of working with high containment. Since biosafety with a
Biosafety. Also, it agents that it involves working with maximum containment.
includes working provides moderate agents indigenous or It consists of working
with agents that risk. This level also exotic that can cause with dangerous agents
provides minimal includes serious lethal disease. that could cause life-
threats to a healthy microorganism Also, air movement are threatening diseases.
individual. related to supervised at this level Also, these diseases
Moreover, most laboratory- due to the potential might come without any
students with lab acquired infections. transmission through existing vaccines or
courses operate at aerosols. treatments.
this level.

Sample Bacillus subtilis HIV Mycobacterium Filovirus and Ebola Virus

Organisms tuberculosis

Pathogen Agents present in Agents that are Present agents at this Agents present are
type this level provides related to human level could either be dangerous with the
least to almost no diseases that tend indigenous or exotic and existing potential to
potential to cause to potentially cause possess the ability to cause fatal disease
harm in both healthy moderate hazard to transmit via aerosol which can be
humans and both aspect; the transmission. Moreover, transmitted through
environment. humans and they are supervised due aerosols.
environment. to its potential to cause
serious lethal disease.

Autoclave None None Pass-thru autoclave Pass-thru autoclave

Requirements

IV. CONCLUSON

The read topic that was about Safety in the Clinical Microbiology Laboratory. It
includes precautions and guidelines associated with the safety of the workers and the
environment towards operating the Laboratory. In conclusion, the presented
precautions and guidelines must be supervised in order to prevent the occurrence of
harm in the Laboratory area. By doing so, agents with potential risk of causing serious
diseases will be prevented.