BIOCHEMISTRY LABORATORY

NAME: Aubrey Cheeza Mae Faith V. Salud DATE: August 11, 2021 (Wednesday)
GROUP NO. ___________________ RATING: _____________

Laboratory Activity 4
DIALYSIS

I- DISCUSSION:
Colloidal solutions, difference between Dialysis and Osmosis and Applications

II- PROCEDURE:

1. In a small cellophane bag, put 5ml of 1% starch solution and 25ml of 1% NaCl solution
2. Tie a string around the top of the bag and suspend it on glass rod laid across your 400ml
beaker containing distilled water, such that the levels of the fluids inside and outside are the
same
3. After 30 minutes, test a few ml. of water in the beaker with a few drops of silver nitrate solution.
Result: The water increased in level; The movement solvent molecules from a
region of lower solute concentration to a region of higher solute concentration
pertains to the osmosis process.
4. Test another portion of the water in the beaker with a few drops of iodine solution.
Result: _The water showed two colors; the iodine solution which has a yellow
color became the color of the bottom part only. It separate the molecules in
solution by difference in their rates of diffusion, this process is what we also call
dialysis.
III- QUESTIONS:

1. What is dialysis?
Dialysis is the separation of suspended colloidal particles from dissolved ions or
tiny molecules (crystalloids) through the pores of semipermeable membranes at
uneven rates of diffusion.

2. What is proven by the results obtained with silver nitrate and starch?
There will be a change of color in to blue - black complex.

3. What is the significance of dialysis in the body?

Dialysis keeps your body in balance when your kidneys fail by eliminating
waste, salt, and excess water and preventing them from building up in the body.
potassium, sodium, and bicarbonate levels in your blood must be kept at a safe
level. assisting in the management of blood pressure

4. Have you observed any errors (personal, method, apparatus types of errors) during the activity
demonstration?
With the video activity demonstration given, they were not using gloves,
although the solutes and solvents are not that dangerous, when handling any
type of chemical and performing any kind of laboratory activity/experiments,
gloves must be worn. Disposable nitrile or neoprene gloves are usually
appropriate as protection from incidental splashes or contact with lab
chemicals.