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  • Experiment 1

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    Maisonette Mich
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    They consist of long chains of amino acids, which are bonded together by peptide linkages and thus called polypeptides. There are about 20 amino acids, and the atoms most prevalent in these are carbon, hydrogen, oxygen, nitrogen, and sulfur. Each amino acid contains a carboxylic acid end and an amino end.

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    EXPERIMENT 1

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    They consist of long chains of amino acids, which are bonded together by peptide linkages and thus called polypeptides. There are about 20 amino acids, and the atoms most prevalent in these are carbon, hydrogen, oxygen, nitrogen, and sulfur. Each amino acid contains a carboxylic acid end and an amino end.

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    © All Rights Reserved
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    6 views1 page

    Experiment 1

    Uploaded by

    Maisonette Mich
    They consist of long chains of amino acids, which are bonded together by peptide linkages and thus called polypeptides. There are about 20 amino acids, and the atoms most prevalent in these are carbon, hydrogen, oxygen, nitrogen, and sulfur. Each amino acid contains a carboxylic acid end and an amino end.

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    © All Rights Reserved
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    EXPERIMENT 1:

    Elementary Composition of Proteins

    MATERIALS:
    ● C81H125N22O39P ● Na2CO3 ● HNO3 ● HCl
    ● CaHNaO2 ● KNO3 ● (NH4)2MoO4 ● BaCl2

    SET-UP:

    TEST (+) RESULT DESCRIPTION SAMPLE

    Carbon & Charring & ● Formation of charring indicates presence of Carbon


    Hydrogen Moisture ● Formation of moisture indicates presence of Hydrogen

    Nitrogen Litmus turned ● Evolution of NH3 gas turns litmus blue


    blue

    FUSION: To be used for sulfur and phosphorus tests


    ● 2 parts Na2CO3 : KNO3 in a crucible
    ● Add C81H125N22O39P and mix
    ● Heat until clear mixture is formed
    ● Cool, then dissolve with warm water, then filter

    Sulfur White ppt ● Presence of white ppt — BaSO4

    3SO42- + BaCl2 —> BaSO4↓ + 2Cl-

    Phosphorus Yellow ppt ● Presence of yellow ppt — (NH4)3PMo12O40

    3NH4 + 12MoO4 + PO43- + 24H+ —> (NH4)3PMo12O40↓ + 12H2O-

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