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  • Ascorbic Acid Schematic Diagram

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    Kat Aquino Eullo
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    This document describes a method for analyzing phosphates using ascorbic acid. The method involves preparing stock and standard phosphate solutions, mixing sulfuric acid, antimony, and potassium tartrate to form a combined reagent, adding the combined reagent to samples and standards and allowing them to react, measuring the absorbance at 880 nm, and plotting absorbance versus concentration to determine the phosphate levels in the samples.

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    This document describes a method for analyzing phosphates using ascorbic acid. The method involves preparing stock and standard phosphate solutions, mixing sulfuric acid, antimony, and potassium tartrate to form a combined reagent, adding the combined reagent to samples and standards and allowing them to react, measuring the absorbance at 880 nm, and plotting absorbance versus concentration to determine the phosphate levels in the samples.

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    © All Rights Reserved
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    6 views1 page

    Ascorbic Acid Schematic Diagram

    Uploaded by

    Kat Aquino Eullo
    This document describes a method for analyzing phosphates using ascorbic acid. The method involves preparing stock and standard phosphate solutions, mixing sulfuric acid, antimony, and potassium tartrate to form a combined reagent, adding the combined reagent to samples and standards and allowing them to react, measuring the absorbance at 880 nm, and plotting absorbance versus concentration to determine the phosphate levels in the samples.

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    © All Rights Reserved
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    Phosphate Analysis using Ascorbic Acid Method

    SMEWW 23rd ed, 4500-P E

    1
    5N sulfuric acid Stock phosphate solution

    Antimony Standard phosphate solution


    2 Mix after
    potassium
    each
    tartrate addition Combined Samples Calibration standards
    reagent
    Ammonium 3
    1 drop phenolphthalein
    molybdate
    Add combined reagent N
    Ascorbic Red color
    4 to sample/standards
    acid present?
    and react for 10-30 min
    Y

    Read absorbance
    Add drop of
    at 880 nm
    5N sulfuric acid and mix

    Plot absorbance vs
    concentration

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