BENEDICT’S TEST

GROUP LACTOSE SUCROSE MALTOSE GALACTOSE FRUCTOSE

1 RED YELLOW- RED RED RED

GREEN

2 YELLOW YELLOW- ORANGE RED BRICK RED

GREEN

3 YELLOW GREEN ORANGE DARK RED

YELLOW

4 YELLOW- BLUE- ORANGE BROWNISH- BROWNISH RED

ORANGE GREEN RED

5 LIGHT-OR LIGHT ORANGE ORANGE DARK

ANGE BLUE ORANGE

6 YELLOW GREEN ORANGE BROWN BROWN

7 YELLOW- GREEN ORANGE ORANGE BROWN

ORANGE

8 YELLOW GREEN RED BROWNISH BROWN

ORANGE ORANGE

9 RED GREEN RED RED RED

FEHLING'S TEST

GROUP LACTOSE SUCROSE MALTOSE GALACTOSE FRUCTOSE

1 RED NO RED RED RED RED

PRECIPITATE

2 YELLOW OLIVE ORANGE RED RED

GREEN

3 YELLOW OLIVE BROWN BROWN BROWN

GREEN ORANGE YELLOW ORANGE

4 RED NO RED RED RED RED

PRECIPITATE

5 LIGHT GREEN RED BROWN RED

ORANGE RED

6 DARK GREEN ORANGE DARK BROWN

ORANGE ORANGE

7 ORANGE GREEN RED RED RED

8 RED OLIVE RED RED RED

GREEN

9 RED OLIVE RED RED RED

GREEN
 RESULTS

TESTS REAGENTS PRINCIPLE POSITIVE RESULTS

Fehling’s (reducing Copper (II) Sulfate, Oxidation, Reduction, Brick red precipitate
sugar) Sodium Hydroxide Reaction = Copper (I)
Oxide

Benedict’s Copper (II) Sulfate, Oxidation, Reduction, Brick red precipitate

(reducing sugar) Reaction = Cu2O

Barfoed’s (reducing Copper (II) Sulfate, Oxidation, reduction Brick red precipitate
monosaccharides) Carboxylic acid

Sellwanoff’s 6N HCl (dehydrating Dehydration, Ketose

(ketoses) agent) condensation Dark red
Resorcinol - Cherry red
condensation
Aldose
Pink

Bial’s (pentoses) Arcinol, ferric Dehydration, Blue-green (bluish)

chloride, hydrochloric condensation Precipitate
acid

RESULTS

TESTS REAGENTS PRINCIPLE POSITIVE RESULTS

Anthrone's H2SO4 (Sulfuric Acid) Hydrolysis, Bluish green

(carbohydrates) - dehydration dehydration (furfural
Anthrone - derivatives),
condensation condensation (bluish
green complex)

Molisch's H2SO4 (Sulfuric Acid) Hydrolysis, Purple colored ring

(carbohydrates) - dehydration dehydration (furfural
alpha-naphthol in derivatives) ,
95% ethanol - condensation(purple
condensation colored complex)

Mucic Acid Nitric Acid Oxidation of White crystal

(galactose and galactose to galactic
lactose) acid

Iodine (starch) Iodine solution Indicator Blue violet precipitate