Professional Documents
Culture Documents
SEDE: CONCEPCIÓN
DOCENTES:
Año 2018
UNIVERSIDAD NACIONAL DE CATAMARCA
FACEN – INGLÉS TÉCNICO
CELL BIOLOGY
Cell biology (formerly called cytology, from the Greek κυτος, kytos, "vessel") is a branch of
biology that studies the different structures and functions of the cell and focuses mainly on the
idea of the cell as the basic unit of life. Cell biology explains the structure, organization of the
organelles they contain, their physiological properties, metabolic processes, signaling
pathways, life cycle, and interactions with their environment. This is done both on a
microscopic and molecular level as it encompasses prokaryotic cells and eukaryotic cells.
Knowing the components of cells and how cells work is fundamental to all biological sciences;
it is also essential for research in bio-medical fields such as cancer, and other diseases.
Research in cell biology is closely related to genetics, biochemistry, molecular biology,
immunology, and developmental biology.
The study of the cell is done on a molecular level; however, most of the processes within the
cell are made up of a mixture of small organic molecules, inorganic ions, hormones, and water.
Approximately 75-85% of the cell’s volume is due to water making it an indispensable solvent
as a result of its polarity and structure. These molecules within the cell, which operate as
substrates, provide a suitable environment for the cell to carry out metabolic reactions and
signaling. The cell shape varies among the different types of organisms, and are thus then
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classified into two categories: eukaryotes and prokaryotes. In the case of eukaryotic cells -
which are made up of animal, plant, fungi, and protozoa cells - the shapes are generally round
and spherical, while for prokaryotic cells – which are composed of bacteria and archaea - the
shapes are: spherical (cocci), rods (bacillus), curved (vibrio), and spirals (spirochetes).
Cell biology focuses more on the study of eukaryotic cells, and their signaling pathways, rather
than on prokaryotes which is covered under microbiology. The main constituents of the
general molecular composition of the cell includes: proteins and lipids which are either free
flowing or membrane bound, along with different internal compartments known as organelles.
This environment of the cell is made up of hydrophilic and hydrophobic regions which allows
for the exchange of the above-mentioned molecules and ions. The hydrophilic regions of the
cell are mainly on the inside and outside of the cell, while the hydrophobic regions are within
the phospholipid bilayer of the cell membrane. The cell membrane consists of lipids and
proteins which accounts for its hydrophobicity as a result of being non-polar substances.
Therefore, in order for these molecules to participate in reactions, within the cell, they need to
be able to cross this membrane layer to get into the cell. They accomplish this process of
gaining access to the cell via: osmotic pressure, diffusion, concentration gradients, and
membrane channels. Inside of the cell are extensive internal sub-cellular membrane-bounded
compartments called organelles.
cell has completed its growth process, and if it is found to be damaged or altered it undergoes
cell death, either by apoptosis or necrosis, to eliminate the threat it cause to the organism’s
survival.
Reference
https://en.wikipedia.org/wiki/Cell_biology
ACTIVIDADES:
3. Extraiga los siguientes tipos de palabras, consigne líneas e interprételas (no menos de
5 de cada categoría):
Palabras transparentes: Biology (L.2) Cancer (L.8) idea (L.3) fundamental (L.41)
indispensable (L.13)
Palabras conocidas: animal (L.19) Bacteria (L.20) proteína (l.24) factors (L.52)
organismos (L.39)
Palabras técnicas: Prokaryotic (L.6) eukaryotic (L.6) Methabolic (L.16) membrana
(L.32) archaea (L.20)
Palabras repetidas: cell, molecules, and, they, of
Palabras clave: Hydrophilic, biology, molecules,
4. Lea el texto en forma global para decidir cuál de las siguientes frases sintetiza mejor el
tema central. Justifique su elección.
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6. Observe el gráfico y mencione los componentes de la célula que puede identificar sin
consultar el diccionario.
Cellia, Lysosome, Cytoplasm, Ribosome, Centrioles
7. Coloque en orden las ideas centrales de los párrafos. Relee las primeras oraciones de
cada párrafo (o también conocidas como oraciones tópicas) para poder resolver este
punto.
8. Con información del primer párrafo, marque correspondencia entre las siguientes
frases.
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FACEN – INGLÉS TÉCNICO
concentration gradients
membrane channels
host
survival
cell growth
12. Observe las palabras en negrita para completar el cuadro. Luego, interprete las frases.
ADJETIVO GRADO DE COMPARACIÓN INTERPRETACIÓN
POSITIVO COMPARATIVO SUPERLATIVO
most of the processes within the cell (l. 12 ) X La mayoría de los
procesos adentro de la
célula
a mixture of small organic molecules (l. 13) X La mezcla de pequeños
organismos
moleculares
Cell biology focuses more on the study (l. 22) X La biología celular se
focaliza mas en el
estudio
as the organism gets larger so too does the X Mientras el organismo
number of cells present(l. 40) se hace más grande,
también lo hace el
número de celular
presentes
13. Identifique F.N. (Frase Nominal) y F.V. (Frase Verbal) de las siguientes oraciones.
Identifique el núcleo de la frase nominal.
● F.N: Research in S.N. cell biology V.P.C: is closely related F.V: to genetics, biochemistry,
molecular biology, immunology, and developmental biology.
● F.N. S.N Cell biology V.P.C: focuses F.V more on the study of eukaryotic cells, and their
signaling pathways, rather than on prokaryotes which V.P.C is covered F.N under
microbiology.
● F.N:The cell membrane consists of lipids and proteins which accounts for its hydrophobicity
as a result of being non-polar substances.
● F.N:The growth and development of S.N: the cell V.P.C: are essential for F.V: the
maintenance of the host, and survival of the organisms.
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a) In a medical sense, IMMUNOLOGY deals with the body’s system of defense against
disease-causing microorganisms and with disorders in that system’s functioning.
d) Although biological LIPID are not large macromolecular polymers (e.g., proteins, nucleic
acids, and polysaccharides), many are formed by the chemical linking of several small
constituent molecules.
UNIVERSIDAD NACIONAL DE CATAMARCA
FACULTAD DE CIENCIAS EXACTAS Y NATURALES
INGLÉS TÉCNICO
TRABAJO PRÁCTICO Nº 2
TEXTO: THE CHEMISTRY OF CELLS
we recognize as living, such as the ability to grow, to move, to reproduce, and to respond
in an informed way to changes in their environment.
DNA (deoxyribonucleic acid) and RNA (ribonucleic acid) (see Nucleic Acids) are the
information-carrying macromolecules that encode the complex form and composition of an
organism and allow it to be perpetuated from one generation to another. Unique among
molecules, they provide directions for their own replication, thereby providing a basis for
the continuity of life. DNA molecules are extremely long polymers made from four
nucleotide bases —adenine, cytosine, guanine, and thymine—often represented by the
letters A, C, G, and T, in a linear sequence. A typical chromosome in a human cell, for
example, consists of two strands of DNA, each of which might contain 50 million to 100
million bases in a unique and precisely determined sequence. The cell has 46
chromosomes of this kind and the same set of chromosomes, each with an essentially
identical base sequence, can be found in every one of the other hundreds of millions of
cells in the body. One of the most exciting developments in recent years has been the
elucidation of the complete sequence of nucleotides in the DNA of many organisms,
including humans (see Human Genome Project).
RNA molecules are built to a similar plan as DNA, but they are much shorter and slightly
different chemically; their four bases are adenine, cytosine, guanine, and uracil rather than
thymine (represented by A, C, G, and U, not T). RNA molecules are made as copies of
selected regions for the DNA, usually for the purpose of making protein.
If we liken DNA to the program, or software, of the cell, then proteins are its hardware: the
physical bricks from which the cell is built. Protein molecules perform a bewildering variety
of functions. As well as providing building blocks, proteins also act as enzymes to catalyze
the myriad reactions inside a cell. Proteins embedded in the plasma membrane form
channels and pumps that control the passage of small molecules into and out of the cell.
Some proteins carry messages from one cell to another while others act as signal
integrators that relay sets of signals from the plasma membrane to the nucleus of
individual cells. Yet others serve as tiny molecular machines with moving parts that propel
organelles through the cytoplasm or untangle knotted DNA molecules. Highly specialized
proteins act as antibodies, toxins, hormones, antifreeze molecules, elastic fibres, ropes, or
sources of bioluminescence.
In chemical terms, proteins are long linear polymers of amino acids joined head to tail by
peptide bonds. In contrast to DNA and RNA, which are made of nucleotide subunits that
are chemically very similar to each other, proteins are built up from an assortment of 20
amino acids that differ greatly in their chemical “personalities”. Each chain of amino acids
folds into a particular shape, or conformation, in which some amino acids are buried on the
inside, and other are exposed to the surrounding water. It is the chemistry of amino acids
on a protein surface that specify its interactions with other molecules and determine its
function, as an enzyme, or structural protein, or whatever. The sequence of amino acids in
a protein, on which its properties depend, is itself specified by the sequence of nucleotide
bases in one particular region of the DNA. A complicated machinery inside the cell first
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FACEN – INGLÉS TÉCNICO
copies this region of DNA into a smaller RNA molecule, and then uses this RNA to direct
amino acids to the machinery that links them together, in the correct sequence.
ACTIVIDADES:
2. Lea el texto atentamente y subraye las palabras clave. Luego, exprese la idea central y
subraye la/s funciones del texto que predominan: NARRACIÓN, RELACIÓN VERBAL-
VISUAL, INFORME, INSTRUCCIÓN, DESCRIPCIÓN, CLASIFICACIÓN, DEFINICIÓN.
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FACEN – INGLÉS TÉCNICO
7
PROPIEDADES tales como la habilidad para crecer, moverse, reproducirse y
responder en una forma 8UNIFORME a los cambios en su 9AMBIENTE
5. Transcriba diez (10) palabras que desconozca y con ayuda del diccionario consigne su
equivalente castellano.
6. Indique la función que cumplen las formas –ing en cada caso. (Sustantivo – Adjetivo –
Verbo). Luego, interprételas.
a. carrying (l. 12) Verbo b. providing (l. 14) Verbo c. exciting (l. 22) Adjectivo
d. including (l. 24) adjectivo e. making (l. 28) Verbo f. moving (l. 36) Adjectivo
macromolecules (l. 7)
subunits (l. 8)
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FACEN – INGLÉS TÉCNICO
11. Responda:
a. Mencione las cuatro bases nucleótidas que conforman las moléculas de ADN.
Adenina, Guanina, Timina, Citosina
b. ¿Cuántas bases tiene cada cadena de ADN? Cada cadena tiene 2 bases.
c. ¿Cuáles son las cuatro bases de las moléculas de ARN? Adenina, uracilo, Guanina,
Citosina.
12. Una las palabras de la columna izquierda con las de la derecha para formar frases.
Luego, interprételas.
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FACEN – INGLÉS TÉCNICO
b. La palabra AND (l. 11) establece una relación de ADICION entre las ideas.
El autor de este texto lo utiliza para conectar las siguientes ideas:
Idea 1: ADN
Idea 2: ARN son la información llevada a las macromoléculas.
d. La palabra BUT (l. 25) establece una relación de CONSTRANSTE entre las ideas.
El autor de este texto lo utiliza para conectar las siguientes ideas:
Idea 1: Las moléculas de ARN son construidas a un plan similar al ADN
Idea 2: son cortas y pequeñas, químicamente diferentes.
15. Analice los pre y posmodificadores de las siguientes frases nominales. Luego,
interprételas.
c. extremely long polymers made from four nucleotide bases (l. 15-16)
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FACEN – INGLÉS TÉCNICO
Lea rápidamente el texto, para corroborar lo que predijo acerca del contenido del
mismo.
Have you ever been inside a greenhouse on a cold winter day? It might be cold outside, but inside
the greenhouse lush green plants flourish in the warmth and sunshine. Greenhouses are made of
glass and are designed to hold heat inside. Our planet's atmosphere also traps energy, sort of like
a greenhouse. Energy from the Sun can enter the Earth’s atmosphere, but not all of it can easily
find its way out again.
What blocks the Sun’s energy from escaping from the Earth? Unlike a greenhouse, the Earth
does not have a layer of glass over it! Instead, molecules in our atmosphere called greenhouse
gases absorb the heat. Greenhouse gases include water vapor, methane, ozone, nitrous oxide,
and carbon dioxide. There may not be much of some of these gases in our atmosphere, but they
can have a big impact. Each greenhouse gas molecule is made of three or more atoms that are
bonded loosely together. These molecules are able to absorb heat, which makes them vibrate.
They eventually release the heat energy and it is often absorbed by another greenhouse gas
molecule.
The greenhouse effect is useful because trapping some energy keeps the temperatures on our
planet mild and suitable for living things. Without its atmosphere and the greenhouse effect, the
average temperature at the surface of the Earth would be zero degrees Fahrenheit. However, too
many greenhouse gases can cause the temperature to increase out of control. Such is the case
on Venus where greenhouse gases are abundant and the average temperature at the surface is
more than 855 degrees Fahrenheit (457 degrees Celsius).
You might hear people talking about the greenhouse effect as if it is a bad thing. It is not a bad
thing, but people are concerned because Earth’s greenhouse is warming up very rapidly. This is
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FACEN – INGLÉS TÉCNICO
happening because we are currently adding more greenhouse gases to our atmosphere, causing
an increased greenhouse effect. The increased Greenhouse Effect is causing changes in our
planet that can affect our lives.
ACTIVIDADES:
a. it (l. 4)
b. they (l. 9)
c. that (l. 10)
d. which (l. 11)
e. them (l. 11)
planet's gases
greenhouse atmosphere
gas degrees Celsius
average temperature
457 molecule
6. Analice los pre y posmodificadores de las siguientes palabras e interprete las frases
analizadas:
● gases (l.9)
● atoms (l.10)
● temperature (l.15-16)
8. Responda las siguientes preguntas. Indique, luego, las líneas de donde extrajo la
información.
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FACEN – INGLÉS TÉCNICO
10. Complete:
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FACEN – INGLÉS TÉCNICO
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A census in 1914 revealed that Argentina had approximately 105 million hectares of
forest. Today, studies estimate a remaining 28 to 45 million hectares. According to the
United Nations (UN) Food and Agriculture Organization (FAO), some 16,288,000 hectares
of Argentina’s forest cover have been lost between 1980 and 2000.
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FACEN – INGLÉS TÉCNICO
ACTIVIDADES:
1. Lea rápidamente el texto y consigne la idea central del mismo. Indique su función.
3. Indique qué significan las siguientes siglas según el texto: UN, FAO, WWF, FVSA.
4. Responda:
a. ¿Cuál es el saldo de hectáreas de bosque que quedan actualmente según estiman
los estudios?
b. ¿Cuál es una de las amenazas para la conservación de yungas?
c. ¿A qué se denomina bioma?
d. ¿Cuáles son las acciones que llevaron a cabo las organizaciones WWF y FVSA en
forma conjunta para combatir los problemas ambientales?
e. ¿De qué se ocupa la FVSA actualmente?
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FACEN – INGLÉS TÉCNICO
The microscope shows us that cells exist in many different sizes and shapes. Some of the
smallest bacterial cells are short cylindrical objects less than 1 µm in length. At the other
extreme, nerve cells have complex shapes including many long thin extensions, and may
reach lengths of several metres (those in the neck of a giraffe provide a dramatic
example). Between these extremes, plant cells are typically 20-30 µm long, polygon-
shaped with box-like boundaries defined by rigid cell walls. Most cells in animal tissues are
compact in shape, 10-20 µm in diameter with an irregular and often richly folded surface.
Despite their many differences in appearance and function, all cells have a surrounding
membrane (termed the plasma membrane) enclosing a water-rich substance called the
cytoplasm. All cells carry out multiple chemical reactions that enable them to grow,
produce energy, and eliminate waste, together termed metabolism (from a Greek word
meaning “change”). All cells contain hereditary information, packed into a central nucleus
and encoded in molecules of deoxyribonucleic acid (DNA), which directs the cell's
activities and enables it to reproduce, passing on its characteristics to its offspring. These
and other similarities too numerous to mention, and including many identical or nearly
identical molecules, demonstrate that all modern cells are related to one another. In other
words, there must have been an unbroken continuity between modern cells—and the
organisms they compose—and the first primitive cells that appeared on Earth.
ACTIVIDADES:
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FACEN – INGLÉS TÉCNICO
4. Indique si las siguientes oraciones son verdaderas (V) o falsas (F) según el texto.
Justifique su respuesta.
6. Observe las palabras en negrita para completar el cuadro. Luego, interprete las frases.
ADJETIVO GRADO DE COMPARACIÓN INTERPRETACIÓN
POSITIVO COMPARATIVO SUPERLATIVO
the smallest bacterial cells (l. 1-2)
short cylindrical objects less than 1 µm in length
(l. 2)
rigid cell walls (l. 6)
many differences (l. 8)
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FACEN – INGLÉS TÉCNICO
8. Marque el sustantivo núcleo de las siguientes frases y consigne si las palabras que lo
modifican son adjetivos (calificativos o determinantes), sustantivos o artículos. Luego,
interprételas.
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FACEN – INGLÉS TÉCNICO
Lea el texto en forma global. Observe el título y los subtítulos; luego, escriba debajo qué infiere
de ellos.
Título: GENERAL FEATURES OF CELLS ………………………………………………
A fundamental division, both in size and in internal organization, exists between prokaryotic cells
and eukaryotic cells. Prokaryotic cells, found only in bacteria and cyanobacteria (formerly known
as blue-green algae), are relatively small (1-5 µm in diameter) and simple; their genetic material
(DNA) is concentrated in one region of the cytoplasm but no membrane separates this region from
the rest of the cell. Eukaryotic cells, from which all other living organisms are made, including
protozoa, plants, fungi, and animals, are much larger (typically 10-30 µm in linear dimension) and
their genetic material is enclosed by membrane, forming a conspicuous spherical body termed a
nucleus. In fact, the name 'eukaryotic' comes from Greek words meaning 'true kernel, or nucleus';
'prokaryotic' means 'before nucleus'.
CELL SURFACE
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FACEN – INGLÉS TÉCNICO
A thin oily skin termed the plasma membrane encloses the contents of all living cells, defining the
boundary between the contents of the cytoplasm and the surroundings. The plasma membrane is
a continuous layer of lipid and protein molecules about 5 nanometres (nm) thick that acts as a
selective barrier to regulate the cell's chemical composition. When lipid molecules such as
phospholipids and cholesterol are mixed with water they spontaneously form thin sheets, or
bilayers, that close up into vesicles. Water-soluble ions and molecules are unable to cross a bilayer
unaided, but can cross if specific carrier proteins or channels made of protein are embedded in the
lipid. In this way the cell is able to maintain concentrations of such ions and small molecules that
are different from those of their surroundings. A different mechanism, involving small membrane
vesicles that add to or bud from the plasma membrane, allows animal cells to secrete or uptake
macromolecules and even particles the size of bacteria across their membranes.
Many other proteins are present in the plasma membrane, the actual mixture being a distinctive
feature of a particular cell type. Membrane proteins can work as enzymes to catalyze specific
reactions or serve to link the membrane to the matrix of proteins on the outside of the cell, or to the
cytoskeleton on the inside. Still other membrane proteins function as receptors that detect
substances such as growth factors in the cell’s environment and signal their presence to the
cytoplasm or nucleus.
The cells of bacteria, yeasts, fungi, and plants usually have, in addition to a plasma membrane, a
relatively thick and sturdy cell wall made of polysaccharides (predominantly cellulose in the case of
higher plants). The cell wall, which is external to the plasma membrane, maintains the shape of the
cell and protects it from mechanical damage, but it also restricts the movements of the cell and
limits the entry and exit of materials.
Although animal cells lack a rigid cell wall, they often secrete a tough “exoskeleton” which can
have a major influence on the form of the cell. In most tissue of the human body, for example, cells
are enclosed in an extracellular matrix composed of tough fibrous proteins such as collagen and
variable amounts of proteoglycans, made of proteins linked to long, highly charged
polysaccharides. The extracellular matrix is especially abundant in connective tissues, where it
forms the basis of bone and cartilage, but can also be found in endothelia, epithelia, nerve, and
muscle.
THE NUCLEUS
The most conspicuous organelle in most plant and animal cells is the nucleus, typically a
membrane-enclosed, roughly spherical body about 5 µm in diameter. Within the nucleus,
molecules of DNA and proteins are organized into long chromosomes, which usually occur in
identical pairs. Chromosomes are too stringy and intertwined to be identified separately unless the
cell is dividing. Just before a cell divides, however, its chromosomes become condensed and thick
enough to be seen as separate structures. The DNA inside each chromosome is a single very
long, highly coiled molecule containing a linear sequence of genes. Genes contain the coded
instructions for the assembly of RNA molecules and proteins needed to produce a functioning copy
of the cell.
The nucleus is enclosed in a two-layered membrane and interaction between the nuclear contents
and the cytoplasm is permitted through holes, called nuclear pores, in this membrane. A
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FACEN – INGLÉS TÉCNICO
specialized region, the nucleolus, is the site of assembly of particles containing RNA and protein,
which migrate through the nuclear pores to the cytoplasm and are modified to become ribosomes.
The nucleus controls protein synthesis in the cytoplasm by sending molecular messengers in the
form of RNA. This messenger RNA (mRNA), as it is called, is made in the nucleus according to
instructions in the DNA, after which mRNA conveys the messages to the cytoplasm via the nuclear
pores. Once in the cytoplasm, the mRNA attaches to ribosomes and a genetic message is
translated into the primary structure of a specific protein.
ACTIVIDADES:
1. Indique si los siguientes enunciados son verdaderos (V) o falsos (F), según la
información ofrecida por el texto. En caso de ser falsos, corríjalos en español.
d. El material genético de las células eucariotas se encuentra rodeado por una membrana.
CORRECCIÓN: ……………………………………………………..
d. a relatively thick and sturdy cell wall made of polysaccharides (l. 28-29)
h. a single very long, highly coiled molecule containing a linear sequence of genes
(l. 46-47)
2. Extraiga verbos en Presente Simple y Voz Pasiva (12 en total). Consigne líneas e
interprételos.
………………………………………….. ……………………………………………..
………………………………………….. ……………………………………………..
………………………………………….. ……………………………………………..
………………………………………….. ……………………………………………..
………………………………………….. ……………………………………………..
………………………………………….. ……………………………………………..
………………………………………….. ……………………………………………..
………………………………………….. ……………………………………………..
………………………………………….. ……………………………………………..
………………………………………….. ……………………………………………..
………………………………………….. ……………………………………………..
………………………………………….. ……………………………………………..
3. Complete:
c. Las células de las bacterias, levadura, hongos y plantas tienen, además de una
membrana plasmática, una pared ………………………………………………………..
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FACEN – INGLÉS TÉCNICO
6. Realice la siguiente actividad a partir del subtítulo “The Nucleus”. Coloque en orden
las ideas centrales de los párrafos. Relee las primeras oraciones de cada párrafo (o
también conocidas como oraciones tópicas) para poder resolver este punto.
composed of limits
separates contain
have made of
restricts divides
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FACEN – INGLÉS TÉCNICO
We have devised many ways to prevent air pollution. How? Some are so simple that you
may not even think of them at the right time. Others are a bit more demanding.
So where can we start reducing air pollution? It makes sense to attack this or any problem
by examining and addressing the most important factors contributing to it.
Be a Smart Driver. What does this mean?
Approximately half of urban air pollution comes from cars and trucks. So we have
identified two easy but very important ways to prevent air pollution – try to drive less and
be a smarter, more conservative driver.
Cutting down on the number and length of trips by car can help cut vehicle air pollution.
With gasoline and diesel prices being so high recently, many people have chosen to go
less distance for their summer vacations and to decrease their distances driven in town.
If you adopt a few smart driving habits this will help reduce your car's total emissions and
expenses and may be uncover hidden benefits like those listed below. Here are some
other ways to prevent air pollution:
Carpool - take turns and save money on parking.
Shop by phone or mail instead of driving - why waste your time?
Obey the speed limit - tickets cost more than gas.
Accelerate gradually - the engine will last longer, too.
Letting your car warm up while stationary can make it consume up to 50 % more fuel.
If you start driving immediately, the engine will reach its working temperature quicker.
Get regular tune-ups for your car - support the smog check program
Keep the air filter in your car clean - perform other routine maintenance in a timely
fashion
Shut off your engine while waiting for a train (or other long waits).
Walk or ride a bicycle when feasible - or try one of these cool electric bikes for fun.
ACTIVIDADES:
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FACEN – INGLÉS TÉCNICO
4. Complete:
7. Responda:
10. Con la ayuda del diccionario, y en función del texto, ubique las palabras que se
detallan a continuación en la columna correspondiente en el cuadro.
many (l. 1), them (l. 2), at (l. 2), makes (l. 3), the (l. 4), factors (l. 4), to (l. 4), this (l. 5),
approximately (l. 6), trucks (l. 6), important (l. 7), driver (l. 8), recently (l. 10), adopt (l.
12), engine (l. 20), a (l. 24).
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