VIVEKANANDHA COLLEGE OF ARTS AND SCIENCES FOR WOMEN

(AUTONOMOUS) ELAYAMPALAYAM, TIRUCHENGODE

DEPARTMENT OF BIOTECHNOLOGY - I M.Sc. BIOTECHNOLOGY

ODD SEMESTER – MICROBIOLOGY

MODEL EXAMINATION – FEB 2022

NAME OF THE COURSE: COURSE CODE: PG DURATION: 3 Hrs

MICROBIOLOGY
MAX MARKS: 75 20P1BTC04 Date: 03/03/2022

SECTION – A (1 X 20 = 20 MARKS) ANSWER ALL THE QUESTIONS

1. Fleming discovers lysozyme in-------------
a. 1931 b. 1911 c. 1921 d. 1941
2. Who discovered the bacteria that cause cholera?
a. Pierre b. Robert Koch c. Louis Pasteur d. Rudolf
Berthelot Virchow
3. Microbes use inorganic compounds as electron donor is called?
a. Chemotrophs b. Organotrophs c. Lithotrophs d. Phototrophs
4. Cell wall biosynthesis is inhibited by antibiotics by inhibiting-----------
a. LPS b. Cellulose c. Peptidoglyca d. proteins
n
5. Which of the following is not found in the kingdom Monera?
a. Organelles b. Organized cell c. Ability to d. Ability to use
structure reproduce energy
6. % Similarity (%S) of each strain to every other strain is calculated by which method?
a.  Intuitive b. Numerical c. Genetic d. DNA
Method Taxonomy Relatedness homology
experiments
7. Which of the following is a substitute for crystal violet in gram’s staining procedure?
a. Methylene b. Bromocresol c. Safranin d. Phenolpthalen
blue green e
8. Which of the following is not used to determine DNA relatedness in the phylogenetic
 system?
a. Thermal b. Genome size c. GC content d. Amino acid
stability of sequences
related DNA
9. Pseudo-murein is present in cell wall of --------
a. Streptococci b. Staphylococci c. Methanococci d. Micrococci
10. Which bacteria have an unusual capsule among the following?
a. Haemophilus b. Klebsiella c. Streptococcu d. Bacillus
influenzae pneumoniae s anthracis
pneumoniae
11. What does a viral DNA becomes after being associated with the bacterial
chromosome?
a. Gene b. Prophage c. Plasmid d. plaque
12. What is the shape of the nucleoid of poxviruses?
a. Circular b. Brick-shaped c. Irregular d. Dumbbell-
shaped
13. Rancidity in spoiled foods is due to-------
a. Lipolytic b. Proteolytic c. Toxigenic d. Saccharolytic
organisms organisms microbes microbes
14. In most of purple bacteria, the light harvesting centers are
a. B 850 & Fe-S b. B 850 & B 875 c. B 845 & B d. B 850 & B830
875
15. Hetrolactic acid bacteria produce------
a. Lactic acid b. Lactic acid + c. Lactic acid + d. Lactic acid +
only H2O + CO2 CO2 alchohol +
CO2
16. The free floating algae are known as---------
a. Phytoplankins b. Benthons c. Sea weeds d. None of these
17. Fixation of atmospheric nitrogen is by means of
a. Biological b. Lightining c. Ultraviolet d. All the above
process light
18. Role of bacteria in carbon cycle is-------
a. Photosynthesi b. Chemosynthesi c. Breakdown d. Assimilation
s s of organic of nitrogen
compounds compounds
19. Sulphur oxidizing bacteria is--------
a. Alcaligenes b. Pseudomonas c. Thiobacillus d. None of these
20. Quorum sensing is used by bacterial cells to determine which of the following?
a. the size of the b. the availability c. the speed of d. the density of
population of nutrients water flow the population

SECTION – B (5 X 5 = 25 MARKS) ANSWER ALL THE QUESTIONS

21. A) Explain about bacterial culture methods.
B) Write a short note on antimicrobial resistance.
22. A) Describe the criteria for classification of microbes.
B) Write a short note on methods of microbial identification.
23. A) Write a short note on Candidasis.
B) Explain the cultivation methods for viruses.
24. A) Explain the significance of Cyanobacteria and Mycobacterium species.
B) Write a short note on general characteristics of Archaea.
25. A) Explain about sulphur cycle with examples.
B) Brief about quorum sensing.

SECTION – C (3 X 10 = 30 MARKS) ANSWER ALL THE QUESTIONS

26. Give detailed account on sterilization methods.
27. Write a detailed account on classification of viruses with typical examples.
28. Write a detailed note on microbial diseases with examples in each class.
29. Give a detailed note on two commercially important microbes with relevant process.
30. Explain the microbial interaction with classical examples.