Central Philippine University

LIFE SCIENCES DEPARTMENT

Jaro, Iloilo City

Name: _Wallace Dave Hajjy Lim Date: _December, 2022

Course: _BSB Microbiology - 3__

Bio 3103 (General Ecology)

Laboratory Exercise No. 3
Identification and Quantification of Zooplankton as Primary Consumers in Aquatic
Ecosystem

I. Objectives

1. To identify the different species of zooplankton present in pond water; and

2. To know the method or technique in quantifying zooplankton present in pond
water.

II. Materials

Research microscope Sampling bottle

Water sample with zooplankton Coulter counter
Sedgwick-Rafter counting chamber Tissue paper
10 ml pipette Books as references for identification
5% formaldehyde/Lugol’s solution
Distilled water

III. Procedures

1. Get 100 ml water sampling containing zooplankton in one of the ponds in CPU
campus using a plastic sampling bottle.
2. Fix water sample with 5% formaldehyde or Lugol’s solution (5-10 minutes).
3. Clean the Sedgwick-Rafter counting chamber using tap water and tissue paper.
4. Put fixed 1 mL water sample in the Sedgwick-Rafter counting chamber using a
pipet or medicine dropper. Be sure that the whole area where the grid lines are
found will be covered by the water sample. No bubbles will be formed within the
area.
5. Using the microscope, examine the water sample.
6. Identify the species of zooplankton present.
7. Count and compute the number of population for each species of zooplankton.
Computations:
Number of species = Total counts/mL
8. Make 3 replicates out of the water sample and get the mean.
9. Illustrate the species of zooplankton present in the water sample.
 Sedgewick Rafter Counting Chamber

IV. Results, Illustrations and Discussion

Data is also provided to you for the computations of the number of species identified and
counted.

Data: Replicate 1: Pseudodiaptomus annandalei – 25, Acartia tsuensis – 15, Oithona sp. – 7,
Brachionus plicatilis – 32, Paracalanus parvus – 10, Tisbe sp. - 3

Data: Replicate 2: Pseudodiaptomus annandalei – 30, Acartia tsuensis – 19, Oithona sp. – 9,
Brachionus plicatilis – 34, Paracalanus parvus – 13, Tisbe sp. - 4

Data: Replicate 3: Pseudodiaptomus annandalei – 27, Acartia tsuensis – 17, Oithona sp. – 6,
Brachionus plicatilis – 37, Paracalanus parvus – 11, Tisbe sp. - 2

Data: Replicate 4: Pseudodiaptomus annandalei – 25, Acartia tsuensis – 15, Oithona sp. – 7,
Brachionus plicatilis – 32, Paracalanus parvus – 10, Tisbe sp. - 5

Data: Replicate 5: Pseudodiaptomus annandalei – 25, Acartia tsuensis – 15, Oithona sp. – 7,
Brachionus plicatilis – 32, Paracalanus parvus – 13, Tisbe sp. - 1

Data: Replicate 6: Pseudodiaptomus annandalei – 25, Acartia tsuensis – 15, Oithona sp. – 7,
Brachionus plicatilis – 32, Paracalanus parvus – 12, Tisbe sp. - 4
V. Conclusions: