BIOCHEM: Enzyme Kinetics

K1
E + S K -1 ES
K2
E + P MICHAELIS MENTEN EQUATION Vmax I
Vmax
Zero Order Kinestics
K1 K2
E + S K -1 ES E + P * (Steady State Assumption) *
Rate=K[S]

* RATEF = RATE D
*
[ET] = [ES] + [E]
1
V0 First Order Kinetics
Rate=K[S]
2 Vmax
ES ES km [S] k 1 [E][S] = k-1 [ES] + k2 [ES] [E] = [ET] - [ES]
EXAMPLES
Statins ¥8 [S] K1 #w BEEBY
km= k-1k+ k2
i
K2 k 1 ([E T]-[ES])[S] = k-1 [ES] + k2 [ES]
- µmµ + •
µµµ•% mm. + ••

HMG-CoA Beza .
K -1 Meza ☒§-zz 1
Reductase COMPETITIVE k 1 [E T][S] - k 1 [ES][S] = [ES](k-1 + k2 ) km [S] 1 km+[S]
[I] How INHIBITION V0 = Vmax [S]
ACE-1 - B-

MAGNET
←
V0 =k2 [ES]
ACE km
•

Vmax=Normal k 1 ([E T.][S]) - ([ES][S]) = [ES](k-1+ k2) 1 km [S]

k1 k1 V V0 = Vmax [S] + Vmax [S]
[ES] = k0
MAO-1 2
☒m¥% + ¥4 HE [E T][S] - [ES][S] = [ES]km
.

[S] 1 km x 1 1
- *mµw V0 = Vmax [S] [S] + Vmax
MAO $BqKg• + + [ES][S] + [ES][S]
•B ••
•
Buzz .

Vmax = k2 [ET]
B o o z [EI] r
[I]
y= m x + b
Oxamic Acid [S] [ESI] NONCOMPETITIVE
INHIBITION
[E T][S] = [ES]km + [ES][S]
-
Allosteric [I] ¥80 -

Hoo No Vmax
LDH Site •oo→
µyµ µtµ%B Product
km=normal
= V0
2
,
Vmax [E T][S] = [ES](km + [S])
od od
(km+[S]) (km+[S])
C.I. = km N.C.I. = Vmax U.C.I. = km & Vmax
r

G3PDH HA Hoo HA [ES] = [E T][S] Vmax Vmax Vmax Vmax

HAH +
Vmax
☒mµ•%
Vmax
Arsenic µaµ +
- -
-

•z •s% C.I.
N.C.I

Phosphoinositide Mesa .

[I]
Boozy .
(km+[S]) V0 V0 V0
U.C.I

1 1
•B→_ 2 Vmax 2 Vmax 1
2 Vmax

[S]= km
•B→_
[ESI] UNCOMPETITIVE
.

Lithium ☒@• No
INHIBITION
k2 V0 = [ET][S] x
k2
Bipolar Disorder ☒Mµ•o% km a

k2 (km+[S]) 1
9
Product Vmax 1 km km [S] km [S] km km [S]
of
Vmax Vmax [S]

.I.

.I.
N.C
C.I.

U.C

I
No

No
= km+[S]

No
2
V0 = k2 [ET][S]
1

ax

ax
V0

ax
1

/Vm

/Vm

/Vm
1 V0

Sarin

km

km

km
V0

¥8 →• ¥3 (km+[S]) 2 Vmax [S] Vmax (km+[S])

:
[S]

i.
Vmax =
•koÑz• + •a← *aµ•• Vmax
-

(Nerve Gas) BEEB

1

BEEZ
. •
•• 1 1
Vmax Vmax
Vmax

V0 = Vmax [S]
2[S] = km + [S]

* *
[I] -1 1 -1
•
1 .
-1 1
SUICIDE - [S] - [S]
Aspirin
km [S] [S]

[IRREVERSIBLE]
sBBAbB← km
km+[S] km [S]

INHIBITION km = [S] AFFINITY Hexokinase

-
Cox 1
* km = [S] = —
2 Vmax *
km = [S] AFFINITY Glucokinase

BIOCHEMISTRY Michaelis Menten Equation &

•

☒ I

¥-1
Lineweaver-Burke plot
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