005/EXAM2-BIO/9/V/2022

SEMESTER EXAM
MIDDLE SCHOOL
AY 2022-2023

Student’s Name

ID Number Grade

BIOLOGY 9700/01
Semester 1 Assessment May 2023
(90 minutes)
Additional Materials: Multiple Choice Answer Sheet
Soft, clean eraser
Calculator
Soft pencil (type B or HB is recommended)

READ THESE INSTRUCTIONS FIRST

Instructions
This paper consists of two parts:
Section A: 20 numbers of Multiple-Choice Questions
Section B: 4 numbers of Structured Questions
For Section A choose the best answers and write the letter of your
chosen answers in the boxes provided on page 2.
For Section B, answer all questions in this book using blue or black pen
only on the spaces provided.
Do all your rough works in this exam paper.
. For Examiner’s Use
Information
Total mark for Section A is 20. Section A
There is no deduction for incorrect answer for these sections.
Total mark for Section B is 25.
The point allocated is stated at the end of each question. Improper
answers, missing units, incorrect rounding (up to 3SF) for calculation Section B
results and or missing work processes will penalize your marks.

Advice Total
Do not spend too long on any question. If you have time at the end, go
back and answer any question you missed out.

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SEMESTER EXAM
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AY 2022-2023
Section A: Multiple-Choice Questions
For each question there are four possible answers, A, B, C, and D. Choose the one you consider to
be correct and write your answers in the boxes provided below.

1 2 3 4 5 6 7 8 9 10

11 12 13 14 15 16 17 18 19 20

1 The fluidity of the cell membrane can be changed by a number of factors.

As the fluidity of cell membranes decreases, which process would be least affected?

A active transport
B diffusion
C endocytosis
D osmosis

2 The diagram represents three possible ways through a cell surface membrane.

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MIDDLE SCHOOL
AY 2022-2023
Which process is represented by each arrow?

3 Which mode of transport is energy independent?

A diffusion
B active transport
C exocytosis
D endocytosis

4 Cells require the following for normal functioning.

1 glucose

2 water

3 amino acids

4 oxygen

Which molecules require a protein for transport across the cell surface membrane?

A 1 only
B 1 and 3
C 1, 2 and 4
D 1, 2, 3 and 4

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AY 2022-2023
5 What form of cellular transport is shown in the diagram below?

A exocytosis
B facilitated diffusion
C protein group
D endocytosis

6 The graph shows the results of an investigation into the effect of amylase on starch at three
different temperatures.

Which statements are correct conclusion using these results?

1 The optimum temperature is 500C

2 The initial rate of reaction is highest at 700C

3 The higher the temperature the more quickly the enzyme denatures.

A 1, 2 and 3

B 1 and 2 only

C 1 and 3 only

D 2 and 3 only

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SEMESTER EXAM
MIDDLE SCHOOL
AY 2022-2023

7 The graph shows the effect of temperature on the rate at which the enzyme in a biological
washing powder digests and removes fruit juices.

Which statements explain the shape of the graph at temperature higher than X?

1 Bonds are broken between the R groups of the amino acids in the
polypeptide of the enzyme.

2 There are more collisions between the enzyme and its substrate.

3 The tertiary structure of the enzyme is altered.

4 The shapes of the active site and the substrate are no longer complementary.

A 1, 2 and 3
B 1, 2 and 4
C 1, 3 and 4
D 2, 3 and 4

8 Which statements about non-competitive inhibitors correct?

1 The inhibitor can bind to the enzyme whether or not the enzyme has its
substrate in the active site.

2 The inhibitor may bind at allosteric site.

3 The inhibitor may not prevent substrate binding at the active site, but will
prevent product formation.

A 1, 2 and 3
B 1 and 2 only
C 1 and 3 only
D 2 and 4 only

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9 The graph shows the effect of an enzyme on a reaction.

Which labels are correct?

A X is the reaction without the enzyme, W is the activation energy.

B X is the reaction with the enzymes, Z is the overall energy change.
C Y is the reaction with enzyme, W is the difference in activation energy.
D Z is the energy gained by the products, W is the activation energy with enzyme.

10 The diagram shows an enzyme, its substrate and an enzyme- substrate complex.

Which statement explain how the substrate is able to enter the active site of the enzyme?

A Contact between substrate and the enzyme causes a change in the enzyme shape.
B The shape of the active site and the shape of the substrate are complementary.
C The substrate within the active site forms hydrogen bonds with amino acids.
D When the enzyme/substrate complex forms, the tertiary structure of the enzyme
changes.

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11 Diffusion is a result of........''

Which option correctly completes the sentence?

A cellular energy forcing molecules to collide with each other.

B cellular energy moving molecules across a partially permeable membrane.
C molecules constantly moving and colliding with each other.
D molecules being attracted to each other.

12 A water- soluble signalling molecule binds to the cell surface membrane, causing the
following processes to occur.

1 Enzyme catalysed reaction

2 2nd messenger production

3 Protein receptor conformational change

4 G-protein activation

Which is the correct sequence of events in the cell signalling pathway?

A 1-2-3-4
B 3-4-1-2
C 1-2-4-3
D 2-4-3-1

13 Which statement describes carrier proteins in cell membranes?

A Carrier proteins are found on the outer surface of the membranes allowing cell
recognition.
B Carrier proteins are involved in moving substances through the membranes by either
active transport or passive transport.

C Carrier proteins allow the binding of ligand molecules, which causes changes within
cells.

D Carrier proteins are involved in moving substances through the membranes by passive
transport through water-filled pores.

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14 What happens to a typical bacterium when it is placed in surroundings that have a higher
water potential than the water potential inside the cell?

A The bacterium bursts because the cell wall has no structural function.
B The bacterium dies because water leaves the cell by osmosis.
C There is no change because the cell wall is impermeable to water.
D There is a net movement of water into the bacterium.

15 When red blood cells are put into pure water, they burst (haemoylsis).

Which statements explain this haemolysis?

1 The water potential of the surrounding liquids lower than the water potential of the
contents of the red blood cell.
2 The cell surface membranes of red blood cells are not supported by cell walls.
3 More water moves into the red blood cells by osmosis than leaves the cells.
4 Water enters the red blood cells by osmosis but does not leave the cells.

A 1 and 3
B 1 and 4
C 2 and 3
D 2 and 4

16 Cholesterol is an integral component of the cell membrane.

Which statement about cholesterol is correct?

A It allows ions to pass freely through the cell surface membrane.

B It has hydrophobic head and a hydrophilic tail.
C It helps to regulate the fluidity of the cell surface membrane.
D It reduces the mechanical stability of the phospholipid bilayer.

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17 Which of these can pass directly through cell surface membrane without using a carrier
proteins or a channel protein?

1 Ca2+

2 CO2

3 C6H12O6

A 1 and 2
B 1 and 3

C 2 and 3
D 2 only

18 Companion cells use ATP to move hydrogen ions out of the cell and co-transporter proteins
to allow hydrogen ions to return with sucrose molecules.

Which two processes are involved in this movement?

A active transport and diffusion

B active transport and osmosis
C exocytosis and diffusion
D exocytosis and facilitated diffusion

19 How does increasing substrate concentration affect the rate of an enzyme-catalysed reaction
in the presence of a competitive inhibitor?

A The rate of the reaction slows.

B The rate of the reaction increases.
C The rate of the reaction slows initially and then recovers.
D The rate of the reaction is not affected by additional substrate.

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SEMESTER EXAM
MIDDLE SCHOOL
AY 2022-2023

20 The graph shows the effect of substrate concentration on the rate of an enzyme-controlled
reaction. The enzyme concentration is constant.

Which statement about the graph is correct?

A Between X and Z, the temperature is limiting.

B Between X and Z, the number of enzyme molecules is limiting.
C Between X and Y, the number of substrate molecules is limiting.
D Between Y and Z, the number of substrate molecules is limiting.

[Total: 20]

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Section B: Structured Questions
Answer the following questions on the spaces provided.

21 (a) The reaction shown below is an enzyme- catalysed reaction.

(i) Name the part of the enzyme labelled U.

......................................................................................................................... [1]

(ii) With reference to Fig.1.2, explain the mode of action of enzymes.

.........................................................................................................................

.........................................................................................................................

.........................................................................................................................

.........................................................................................................................

.........................................................................................................................

.........................................................................................................................

......................................................................................................................... [4]

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22 One role of the cell surface membrane is to control the entry and exit of substances.

(a) Compare the Table 1.1 to show the transport mechanism across cell surface
membranes and example of materials transported.

Table 1.1

[2]

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AY 2022-2023
(b) Each transport mechanism across cell surface membranes has a characteristics
set of features.

In each of the boxes below, state one example of a transport mechanism that
matches the pathway shown.

[4]

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MIDDLE SCHOOL
AY 2022-2023
23 The fluid mosaic model of membrane structure was first proposed in 1972by Singer and
Nicolson. The model describes in detail how the components of a membrane are
organised.

(a) Some of the components of the cell membrane are:

 phospholipid molecules

 protein molecules

 cholesterol molecules

(i) In the box below, draw a labelled diagram of a section through a cell surface
membrane to show how the above components are organised within the
membrane.

The diagram should include other named components of the membrane.

Label the inner and outer surfaces of the membrane.

[5]

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MIDDLE SCHOOL
AY 2022-2023
(ii) Suggest why "fluid mosaic" is an appropriate term to use to describe
membrane structure.

.........................................................................................................................

.........................................................................................................................

.........................................................................................................................

.........................................................................................................................

.........................................................................................................................

.........................................................................................................................

......................................................................................................................... [4]

24 Fig. 1.1 is a diagram of a cell surface membrane

Fig. 1.1

Use a label line and the appropriate letter to label each of the following on Fig.1.1

P protein for active uptake of potassium ions

Q protein for facilitated diffusion of polar molecules

R receptor site for a hormone

S hydrophilic heads of phospholipids on the internal surface of the membrane

T molecule that modifies the fluidity of the membrane [5]

[Total Marks : 55]

END OF ASSESSMENT

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