Name: Date:

Section: Score:

Experiment No. 2
Colloids

OBJECTIVES: At the end of the experiment, students are expected to learn how to:
1. Demonstrate and prepare the two types of colloidal system.
2. Demonstrate the differences between emulsoid and suspensoid in terms of
properties and reactions.

Discussion:
Colloidal solution is otherwise known as colloidal suspension. A colloid is a
microscopic material that is evenly distributed in another material.

There are two types of colloidal solutions being classified as emulsoid and suspensoid.
While emulsoids are lyophilic they are attracted to solvents. When in contract with a
liquid solution then tend to take up large amount of it causing a swell and form a gel.
A suspensoid on the other hand is lyophobic meaning it has no affinity for solvents.

Emulsoid are viscous and form permanent foam when shaken vigorously with air.
They are precipitated by electrolytes on in high concentrations. Suspensoids, on the
other hand, do not form permanent foam they are precipitated by electrolytes.

Suspensoid particle in water carry electrical charges and are precipitated by ions that
carry charges of opposite sign but not by ions that carry the same sign. There is a
difference, however, between the monovalent and bivalent ions.

Colloids, which carry charge of opposite signs mutually precipitate on another.

Reagents/Materials:
Liver 5% TCA Ethly Alcohol
Gelatin Ammonium Sulfate MgSO4 solid
0.45N NaCl solution Ferric Chloride 0.05N AgnO3 solution
0.45N NaCl solution 1N MgCl2 solution 10% MgSO4 solution
1N Na2SO4 Concentrated nitric acid
Apparatus: Weighing balance, spatula, beaker (250mL), Hot plate, glass rod, test
tubes, test tub holder, test tube rack, test tube brush, graduated cylinder (10mL),
stopwatch, glass dropper

PROCEDURE:
A. Preparation of an Emulsoid
a. In a beaker, dissolve 10g of gelatin in 200mL hot water by consistently stirring
b. Pour 1mL of the prepared emulsoid into a test tube and cool under tap water.
NOTE WHAT IS PRODUCED.
c. Bring the test tube with the emulsoid into the water bath and heat again. Observe
the result. Record your observation in the Data Table.
B. Preparation of suspensoid
a. Place 200mL of boiling water in a beaker
b. Add 1mL of saturated ferric chloride solution. NOTE WHAT IS PRODUCED.
c. Repeat the last two procedure in Part A. Compare the result with those in Part A.

C. Foam Formation
a. Shake 10mL of the 5% gelatin (Emmulsoid) vigorously with air.
b. Stand and note the result after 5minutes. Does it form permanent foam?
c. Shake 10mL of the colloidal ferric chloride solution (Suspensoid) vigorously with
air.
d. Stand and note the result after 15minutes. Does it form permanent foam?

Is there a difference in the foam formation of the two solution? Describe it.
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D. Precipitation with Electrolytes

a. Place 5mL of 5% gelatin solution in a test tube.
b. Add saturated ammonium sulfate drop by drop, until a permanent precipitate is
formed
c. Repeat the procedure using colloidal ferric chloride instead of gelatin and note
the result.

Observe and give the difference. Explain.

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E. Reversibility
a. Place 5mL of colloid ferric chloride in a test tube and add 1mL of 10% MgSO4
solution.
b. Allow to stand for ½ hour.
c. Treat 5mL of 5% gelatin solution in the same way.
d. If no precipitate forms in the latter, add solid MgSO4 until saturated.
e. Decant the supernatant fluid from each of two colloids.
f. Add an excess of water and note whether two colloids are reversible. Note down
the results and explain.

F. Precipitation of Suspensoid Particle with monovalent and Bivalent Ion which

Carry Charges of Opposite Signs
a. Place 5mL of 1% colloid ferric chloride solution in a test tube.
b. Add 0.45N NaCl solution a drop at a time counting the drops and shaking after
each addition until a permanent precipitate is formed. Note down the results.
c. Perform the same procedure nut using 1N solution of Na2SO4 and MgCl2.
Compare the result with those obtained with NaCl.
 G. Protective Action of Emulsoid
a. Place 5mL of 0.05N AgNO3 in the test tube
b. Add 5gtts of concentrated nitric acid
c. Then add 5mL of 0.45 NaCl note the copious precipitate
d. Repeat the experiment but add 1mL of 5% gelatin solution to each of the NaCl
and AgNO3 solution before mixing. NOTE THE RESULTS and EXPLAIN.

Procedure Observations

A. Preparation of an Emulsoid When cooled:

When heated:

B. Preparation of Suspensoid When cooled:

When heated:

C. Foam Formation Gelatin-Emulsoid:

FeCl3-Suspensoid:

D. Precipitation with Electrolytes Number of drops of ammonium sulfate added

to form a permanent precipitate:

Gelatin-Emulsoid:

FeCl3-Suspensoid:
Appearance of Precipitate:

Gelatin-Emulsoid:

FeCl3-Suspensoid:

E. Reversibility Number of drops of (ammonium sulfate)

added to form a permanent precipitate:

Gelatin-Emulsoid:

FeCl3-Suspensoid:

F. Precipitation of Suspensoid particle with

Monovalent and Binovalent Ions which Carry
Charges of Opposite Signs
G. Protective Action of Emulsoid

CONCLUSION:
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Post-Laboratory Questions:

1. How can colloids be made to settle? What use is made of this process?
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2. Why can’t colloids pass through membranes but it does with filter paper?
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3. Differentiate between Emulsoid and Suspensoid in terms:

1) Affinity for solvent
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2) Foam formation
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3) Precipitation with Electrolytes

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4) Reversibility
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References:
Campbell, M.K., & Farell, S.O. (2015). Biochemistry (8th ed.). (R. Lee, Ed.) Singapore:
Cengage Learning Asia.
Zumdahl, S.S., & DeCoste, D.J. (2015). Introductory Chemistry; A Foundation (8th ed.). (M.
Finch, Ed.). United State of America: Cegage Learning.