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N-Based, P-Based, and K-Based Organic Liquid Fertilizer on


Improving the Growth of Water Spinach (Ipomoea aquatica)

A Research Paper

Presented to the Faculty of FEU Roosevelt Cainta

Secondary Education Department

In Partial Fulfillment of the Requirements for Graduation from


Senior High School

Presented by:

Group Number 3 of

Grade 12 – Science, Technology, Engineering, and Mathematics


(STEM)

Agustin, Crystal Jean B.

Chua, Roven C.

De Leon, Juan Miguel Nathaniel

Esperida, Rodrigo

Mendoza, Albert F.

Morata, Beyonce Mikaela D.P.

Palijado, John Emmanuel R.

Villanueva, Hael Olynne Chyle I.

June 2021
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FEU ROOSEVELT CAINTA


Secondary Education
Department
Sumulong Highway, Cainta, Rizal
Telephone No.: (02) 681 – 6210 (Local 129)

APPROVAL SHEET

This thesis “N-BASED, P-BASED, AND K-BASED ORGANIC LIQUID FERTILIZER ON


IMPROVING THE GROWTH OF WATER SPINACH (IPOMOEA AQUATICA)”, prepared and
submitted by Group 3 of Grade 12 – Science, Technology, Engineering and Mathematics (STEM) in
partial fulfillment of the requirements for graduation from Senior High School has been examined and
recommended for acceptance and approval.

June 2021 MR. KHISHI I. NISHINO


Research Adviser

PANEL OF EXAMINERS

MS. KIMBERLY F. BRENIO


CHAIRPERSON OF THE PANEL

MS. JOANA C. CAPINDIT


RESEARCH PAPER CRITIC

MS. CRISELLE JOY B. UBALDO


FACULTY REPRESENTATIVE

Accepted and approved in partial fulfillment of the requirements for graduation from Senior High
School on the __th day of _________, 20___.

MR. JOHN CRISTOPHER B. UBALDO


OIC, Basic Education Division
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Table of Contents

Acknowledgement. . . . . . . . . . . . . . . . . . . . . . v

Dedication . . . . . . . . . . . . . . . . . . . . . . . . vi

Abstract . . . . . . . . . . . . . . . . . . . . . . . . . vii

Chapter I: The Problem and its Background

Introduction. . . . . . . . . . . . . . . . . . . . . 1

Background of the Study . . . . . . . . . . . . . . . 4

Theoretical Framework . . . . . . . . . . . . . . . . 9

Conceptual Framework. . . . . . . . . . . . . . . . . 12

Statement of the Problem. . . . . . . . . . . . . . . 14

Hypothesis. . . . . . . . . . . . . . . . . . . . . . 15

Scope and Delimitation of the Study . . . . . . . . . 15

Significance of the Study . . . . . . . . . . . . . . 17

Definition of Terms . . . . . . . . . . . . . . . . . 21

Chapter II: Review of Related Literature and Studies

Related Literature. . . . . . . . . . . . . . . . . . 22

Related Studies . . . . . . . . . . . . . . . . . . . 27

Chapter III: Research Design and Methodology

Methods of Research . . . . . . . . . . . . . . . . . 30

Respondents / Subject of the Study. . . . . . . . . . 31

Locale of the Study . . . . . . . . . . . . . . . . . 31

Research Instruments. . . . . . . . . . . . . . . . . 32

Data Gathering Procedure. . . . . . . . . . . . . . . 33

Statistical Tools / Treatment of Data . . . . . . . . 36


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Chapter IV: Presentation, Interpretation and Analysis of Data

Problem # 1: [What is the average and standard deviation of

each of the four (4) setups in terms of the following:

a)Fresh leaf mass, b) Dry leaf mass, c) number of leaves,

d) final height] . . . . . . . . . . . . . . . . . . . . . .

. 39

Problem # 2: [Is there a significant difference between the

fresh leaf masses of the four (4) setups?]. . . . . . 42

Problem # 3: [Is there a significant difference between the

dry leaf masses of the four (4) setups?]. . . . . . . 44

Problem # 4: [Is there a significant difference between the

number of leaves of the four (4) setups?] . . . . . . 46

Problem # 5: [Is there a significant difference between the

final heights of the four (4) setups?]. . . . . . . . 47

Chapter V: Summary, Conclusions, and Recommendations

Summary . . . . . . . . . . . . . . . . . . . . . . . 50

Conclusions . . . . . . . . . . . . . . . . . . . . . 52

Recommendations . . . . . . . . . . . . . . . . . . . 60

References . . . . . . . . . . . . . . . . . . . . . . . . 62

Appendices

Appendix A (Data Sheet) . . . . . . . . . . . . . . . 71

Appendix B (Solutions). . . . . . . . . . . . . . . . 74

Appendix C (Documentation). . . . . . . . . . . . . . 88

Curriculum Vitae . . . . . . . . . . . . . . . . . . . . . 91
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Acknowledgement

The researchers would like to express their gratitude to

these following people:

Mr. Khishi I. Nishino, for wholly giving his valuable

knowledge in teaching the fundamental notions in Inquiries,

Investigation, and Immersion (III); to tend to the student's

needs. He has supervised the students in completing their

corresponding research papers.

Subject teachers of 12-STEM, for being kind, understanding,

and encouraging in providing the researchers' adequate

opportunity to accomplish what is required in completing this

research.

FEU Roosevelt Cainta, for being the foundation of the

researchers in honing and sharpening their skills with quality

that is essential for this research's completion.

Family and friends, for being the leverage in pulling the

researchers above the limits that helped us produce the results

of this research and present people the relevant knowledge about

this research.

God Almighty, for providing wisdom, strength, and guidance

to the researchers for the accomplishment of the study.


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Dedication

This study is dedicated to the farmers affected by various

natural disasters. They can create their organic liquid

fertilizer with the help of the knowledge and information

provided by this study so that they can recover from the damages

dealt by storms and floods. This study is also dedicated to

people interested in growing plants at home, such as plantitos

and plantitas. They can use the information in this research as a

reference in making homemade organic fertilizers that can be

applied to their home-grown plants. Lastly, the researchers

dedicate this study to the environment to lessen the use of

chemical-based fertilizers that harm the environment and to

promote organic farming for better sustainability in agriculture.

Abstract

This research has investigated the utilization of organic

liquid fertilizers concentrated with the essential elements for

plant growth, such as nitrogen, phosphorus, and potassium, to

water spinach. The researchers created N-based organic liquid

fertilizer made with pumpkin seeds, P-based organic liquid

fertilizer made with green beans, and K-based organic liquid

fertilizer made with a baked potato. A total of 40 water

spinaches are planted and were subjected to the following

fertilizers. When the plants are harvested, the researchers


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measure the following necessary data: fresh leaf mass, dry leaf

mass, number of leaves, and final height. All data were listed in

a data sheet wherein the researchers used various statistical

tools to interpret the gathered data. The statistical tools that

were applied in this study are based on the findings of this

study; the following conclusions were drawn.

1. The results in calculating the average and standard

deviation of the four setups are the following:

a) The N-based setup has the highest mean in terms of

fresh leaf mass, while the no fertilizer setup has the

lowest mean. Furthermore, the average distance between

the data points and the mean of each component is

small.

b) The N-based setup has the highest average dry leaf

mass, while the no fertilizer setup has the lowest.

Additionally, the data points and the mean of each

component are close to one another.

c) The P-based setup has the highest mean in terms of

number of leaves, while the no fertilizer setup has the

lowest. In addition, the data points have a short

distance between the mean of each setup.

d) The N-based setup has the highest average final height,

while the no fertilizer setup has the lowest. Moreover,


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the data points in each setup are widely scattered.

2. The no fertilizer setup and the concentrated organic liquid

fertilizers have a significant difference in terms of fresh

leaf mass. After comparing each setup, it was proven that

all organic fertilizers are significantly better than the no

fertilizer setup. Among the three organic fertilizers, the

N-based organic fertilizer is the best performing.

3. The no fertilizer setup and the concentrated organic liquid

fertilizers have a significant difference in dry leaf mass.

When each setup was compared, it was proven that all organic

fertilizers performed better than the result of no

fertilizer setup. At the same time, the performance of each

organic fertilizer is equal.

4. The no fertilizer setup and the concentrated organic liquid

fertilizers have no significant difference in number of

leaves, and this means all setups performed equally.

5. The no fertilizer setup and the concentrated organic liquid

fertilizers have a significant difference in terms of final

height. After comparing each setup, it was proven that

organic fertilizers are better than the no fertilizer setup


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except the N-based. Additionally, the P-based and K-based

organic fertilizers have the best performance.


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Chapter I

THE PROBLEM AND ITS BACKGROUND

Introduction

When Super Typhoon Rolly (also known as the world's

strongest typhoon for the year 2020) struck the Philippines, it

brought devastating winds that dealt massive damages to various

infrastructures and, most notably, agriculture. In Bicol province

alone, the Department of Agriculture (DA) stated that Rolly

annihilated ₱ 1.1 billion worth of crops. Also, they estimated

that 17,059 hectares with 65,833 metric tons of agricultural

products were severely affected (The Inquirer Staff, 2020).

While on a national scale, the National Disaster and Risk

Reduction Management Council (NDRRMC) said that over ₱11 billion

worth of infrastructure and agricultural products and facilities

were destroyed (Peralta-Malonzo, 2020). Considering the amount of

damage dealt by Super Typhoon Rolly, it is evident that farmers

and farming companies suffer from severe injuries inflicted by

natural disasters that can likely affect the Philippines' state.

As a solution for the problem, the researchers aim to

produce organic liquid fertilizers that are rich in essential

elements for plant growth. Organic liquid fertilizers are

solutions rich in nutrients that are essential for plant growth.

They derived vital nutrients from fermented organic materials and


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natural products. Additionally, due to their precise

composition and easy utilization, organic liquid fertilizers can

penetrate the soil and deliver nutrients to the plant because of

their fluid nature. Moreover, one-liter packing of a

commercialized organic liquid fertilizer contains 12%-18% of

humic acid, 15%-20% of fulvic+ acid, and 15%-30% of seaweed gel

as micronutrients, 20%-30% of amino, 20%-25% of nitrobenzene, and

plant growth regulator (PGR Liquid) a combination of seaweed,

humic, fulvic brassinolide, and chelated elements (Agri Bio

Chemicals, 2014).

Furthermore, organic fertilizer is recognized as organic

when it only contains organic matter such as animal and plant

matter or by-products of a living organism. Some examples are

bone meal, blood meal, feather meal, meat meal, fish meal,

seaweed meal, or any extracts of these meals. In parallel, water-

soluble building blocks of proteins, which are amino acids, can

also be utilized as organic fertilizers (LebanonTurf, 2013).

The organic liquid fertilizers will be administered to water

spinach. Kangkong, regularly known as water spinach, is a yield

that has filled in Asian societies for ages—loaded up with health

benefits, from a critical presence of dietary filaments to

significant measures of fundamental nutrients. It is known for

being an incredible supplier of nutrients and minerals. Water


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spinach is a superb wellspring of nutrient A, which is essential

for skin, hair, and vision. Aside from nutrients, water spinach

is a primary wellspring of fundamental minerals like iron,

magnesium, and calcium. Magnesium and calcium are essential for

the soundness of bone and teeth. Water spinach contains 21% of

your day-by-day calcium and 18% of your everyday magnesium per

100 grams. Also, water spinach is known as an incredible

wellspring of phosphorus. Water spinach is likewise incredibly

wealthy in electrolytes, like sodium and potassium. One hundred

grams of water spinach can represent 7.5% of your daily sodium

admission and 6.6% of your daily potassium consumption (East-west

seed group, 2018).

Furthermore, the researchers wanted to explore if developing

an organic liquid fertilizer rich in potassium, phosphorus, and

nitrogen that can improve plant growth. This innovation might

help farmers and agricultural companies to recover from the

damages dealt by natural disasters. During the water spinach

development stages, the researchers aim to use organic liquid

fertilizers to experiment on water spinach subjects. The

researchers envision that this particular subject can give

valuable data to prove their effectiveness and reliability. Thus,

this study would determine the effectiveness of the utilization

of organic liquid fertilizer. The research can then bring new

knowledge to provide helpful information regarding growing plants


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using organic fertilizers to farmers and agricultural companies

that struggle to bounce back after a calamity.

Background of the Study

In Southeast Asia, the Philippines is among the most brutal

punished by catastrophic events, especially storms, floods, and

dry seasons. These natural calamities ecologically affect the

influenced zones and individuals who live there. Moreover, the

agribusiness and common assets areas are exceptionally powerless

because of the direct exposure to natural catastrophes and

unwanted outcomes (D.C. Israel, R.M. Briones, 2012).

Due to all the damage caused by natural calamities, one of

the possible solutions, especially in the country's agriculture,

is fertilizer use. Fertilizers supply the supplements that yield

eliminate from the soil. It enhances the soil's supplement stocks

with minerals that can be immediately consumed and utilized by

crops. It furnishes plants with a wide range of supplements that

they need to develop further and sound, including nitrogen,

phosphorus, and potassium.

The fertilizer is a natural or artificial substance

containing chemical elements that improve plants' growth and

productiveness. The utilization of excrement and manures as

composts is presumably nearly as old as agribusiness. Most

nitrogen manures took from manufactured smelling salts. This


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substance compound is utilized either as a gas or in a water

arrangement of regular or counterfeit beginning that is applied

to soil or to plant tissues to supply at least one plant

supplement vital for the development of plants (Stewart, R.

E.,2018).

Organic fertilizers accumulate from the remains or by-

products of organisms. The fertilizer is rich in the three

essential nutrients but unequal proportions. However, organic

fertilizers do not negatively affect the soil as chemical

fertilizers sometimes do. It improves the soil's structure,

movement of water and feeds beneficial microbes. However, it may

cost more materials than chemical fertilizers because they are

less concentrated (Pokorny, 2015).

Organic fertilizers have started to draw in the expanding

consideration of ranchers from young nations. They help to get a

more significant yield level without applying pesticides and

other fake fertilizers that antagonistically influence the nature

of completed items.

Mesopotamia can likewise flaunt the utilization of waterway

mud and peat-sediment as fertilizers for horticultural terrains.

Theophrastus from Eresos, a well-known botanist and biologist on

those far off days, depicts in his works the requirement for

preparing the soil in the development of vegetable harvests and,


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obviously, wheat. It was this item he gave the unique profile in

his book.

During the time of feudalism in Western Europe, in

particular between the eleventh and thirteenth hundreds, once

dispatched the starting to the dynamic extension of planting

acreages, which on occasion were of little reasonableness for

these reasons because of the severe level of peat arrangement.

Natural fertilizers can renew and reestablish the humus

layer and expand the measure of supplements just as the soil

structure. The experience of the created nations shows that the

higher the level of their turn of events, the more consideration

returned to utilizing, specifically, natural fertilizers, to

which the top job in this interaction allotted (Synagra Group,

2017).

An existing study of the Effects of Organic and Inorganic

Fertilizer on Nutritional Composition of Amaranthus spinosus L.

by A.A.J. Mofunanya. J.K. Ebigwai. O.S. Bello, and A.O.

Egbe (2015), as introduced in Table 1.1, the result uncovered

that organic fertilizer delivered fundamentally ( p<0.05 ) higher

impact on crude, fiber, protein, ash, fat, vitamin C and an in

all the plant parts when contrasted and inorganic fertilizer.

Mean values recorded for the above boundaries in the leaf test

were 3. Mean values for ash, fiber, and vitamin C were higher in
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the root and inflorescence. The relative impact of organic and

inorganic fertilizer on the mineral organization of Amaranthus

spinosus is scrutinized in Table 1.2. Results showed that organic

fertilizer created essentially ( p<0.05 ) higher Zn, Cu, Mg, K, Fe,

Ca, Na, and P than inorganic fertilizer in all the plant tests

considered.

Table 1.1. Comparative study of the effect of organic and inorganic fertilizer

on proximate composition of Amaranthus spinosus L. (mg/100 g dry matter)

Values are Mean±SD, n=3 replicates, p<0.05 . Percentage was

obtained by expressing the difference between the values for

control and organic/inorganic fertilizer as a percentage of the

control.
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Table 1.2. Comparative study of the effect of organic and inorganic fertilizer

on mineral composition of Amaranthus spinosus L. (mg/100 g dry matter)

Values are Mean±SD, n=3 replicates, p<0.05 . Percentage

difference was obtained by expressing the difference between the

value for control and organic/inorganic fertilizer as a

percentage of the control.

The researchers chose this study to make sustainable and

agricultural practices that incorporate a diverse construction of

farming methods from producing organic liquid fertilizer that is

the fundamental nutrient in plants; N, P, and K to upgrade soil

productivity and supplement crop efficiency.


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Theoretical Framework

Theory of Mineral
Nutrition of
Plants

Phosphorus Potassium
Nitrogen Organic
Organic Organic Liquid
Liquid Fertilizer
Liquid Fertilizer Fertilizer

Utilization of Effects of Using


Organic Liquid Organic Liquid
Fertilizers to Fertilizer to
Water Spinach Water Spinach

Improvement in
Excessive quality and
Inadequate Use quantity of
Application
water spinach

Sustainable crop
production and
Law of Minimum Trofobiose Theory
preservation of
agrosystem

Better vegetative
Vulnerable to growth and soil
Slow growth
infection characteristics

Figure 1.2. The Theoretical Framework of the study.

The "Theory of Mineral Nutrients of Plants" produced by Carl

Sprengel but later reformed, and Justus von Liebig stated. It

says that certain minerals are needed to be present in a plant to

ensure its growth and soil fertility. The common minerals are

nitrogen (N), phosphorus (P), and potassium (K)(Böhm, Kirkham, &


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van der Ploeg, 1999). The researchers attempt to create three

organic liquid fertilizers concentrated on these three minerals

that are essential in plant growth.

There is a tendency that the utilization of organic liquid

fertilizer is ineffective due to the inadequacy of the

fertilizer's amount or quality. There is a possibility of things

not yielding the desired result should be acknowledged. According

to Justus von Liebig's "Law of the Minimum," if one of the

essential plant nutrients is insufficient, plant growth will be

poor even when all other essential nutrients are abundant. Also,

both slow-release and fast-released fertilizers have advantages

and disadvantages that affect the plant's growth. Unbalanced

rate, combination, and quantity of nutrients to the soil and the

plant would lead to adversity.

The researchers would be utilizing the concentrated organic

liquid fertilizers to the water spinach to make the research

progress. An application would be a significant factor to avoid

over-usage of minerals. As stated in the Trofobiose Theory by

Wagner L. Polito, vegetal organisms or food crops would become

vulnerable to infestation and diseases like insects due to

excessive substance use. By further studying the water spinach

with the experiment, researchers would find not only how organic

liquid fertilizers contributed to the plant's growth.


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As a result of applying the elements of organic liquid

fertilizer such as nitrogen, phosphorus, and potassium, there

will be a noticeable difference in crop production in contrast

with non-fertilized soil. The organic liquid fertilizer will

affect the water spinach viability and its reproduction on

fertilized soil(Drãhici, 2015). Due to the concentrated organic

liquid fertilizer, the soil will become enriched with sufficient

plant growth and development nutrients (Bermejo, Legaz, Martínez-

Alcántara, Martínez-Cuenca, & Quiñones, 2016). Since the soil

have the essential elements, the agrosystem within the soil is

preserved, resulting in sustainable crop production (Fiore et

al., 2013). The researchers aim to promote organic farming and

reduce the use of chemical-based fertilizers that tends to harm

the environment by creating an organic fertilizer that utilizes

little to no chemical or acidic components.

Conceptual Framework

Input
Pumpkin seeds, baked Process Output
potato, and green The creation of N-based, Observing, comparing,
beans as the main P-based, and K-based and contrasting on the
ingredients for each organic liquid fertilizer significant differences
creating a specific all the ingredients. occurred on water
concentrated organic Application of organic spinaches that are
liquid fertilizer to each
liquid fertilizer namely: subjected to N-based, P- 13
nitrogen, phosphorus, water spinach with their based, K-based and no
Figure 1.1. The Conceptual Framework of the study.
and potassium. respective component fertilizer setup.
The The independent (Nitrogen, Potassium,
experimentation started by All of theinformation
gathering observation
variables of the study Phosphorus). will revolve on water
regarding the respective elements; Nitrogen, Phosphorus, and
are the N-based spinach subjects which
Potassium
fertilizer, P-components
based and choosing pumpkin seeds,arebaked
the potato and
dependent
fertilizer, and K-based variable of the
green beans as the main ingredients that contain a study.
relative
fertilizer.
amount of the element for each concentrated organic liquid

fertilizers. After the deliberation, the researchers began to

gather all the ingredients to create the concentrated organic

liquid fertilizers. Simultaneously, all subjects were planted

ahead of time in the same location to ensure that all the test

subjects will have an equal timeframe, type of environment, soil

type, and temperature to grow and develop.

The creation of concentrated organic liquid fertilizer began

by assembling all the ingredients. By using the methods found in

relevant articles, the product would undergo fermentation to

break down the components for better absorption of nutrients

within each element. When the fermentation period is finished,

the concentrated organic liquid fertilizers is ready to fertilize

the experimental subjects. However, the organic liquid fertilizer

will only be used respectively on the specific water spinach

growth stage that the professionals recommend. The concentrated

organic liquid fertilizers will be applied until the water

spinach experimental subjects reach harvest time. Furthermore,


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the observations will be logged after the plants are harvested.

The gathered data will be then interpreted for the study’s

conclusion.

The researchers are taking the timeframe as an essential

factor in this study, including fermentation and application

days. concentrated organic liquid fertilizers is a fertilizer

that needs a sufficient time to develop and improve the water

spinach growth. After the given timeframe for the test subjects

to grow and develop, all observation will be recorded on data

sheets to compare the results of each setup to one another. This

study will be considered a success if there are significant

differences between the three experimental water spinach subjects

and controlled water spinach subjects.

Statement of the Problem

The study aims to determine the effects of utilizing concentrated

organic liquid fertilizers to water spinach growth. These

research questions below will provide further information that

the researchers would like to find out:

1. What is the average and standard deviation of each of the

four (4) setups in terms of the following?

a) Fresh leaf mass

b) Dry leaf mass


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c) Number of leaves

d) Final heights

2. Is there a significant difference between the fresh leaf

masses of the four (4) setups?

3. Is there a significant difference between the dry leaf

masses of the four (4) setups?

4. Is there a significant difference between the total

number of leaves of the four (4) setups?

5. Is there a significant difference between the final

heights of the four (4) setups?

Hypothesis

1. There is no significant difference between the no fertilizer

setup and the three concentrated organic liquid fertilizers

in terms of fresh leaf masses.

2. There is no significant difference between the no fertilizer

setup and the three concentrated organic liquid fertilizers

in terms of dry leaf masses.

3. There is no significant difference between the no fertilizer

setup and the three concentrated organic liquid fertilizers

in terms of total number of leaves.

4. There is no significant difference between the water-only

setup and the three concentrated organic liquid fertilizers

in terms of final heights.


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Scope and Delimitations

The study will focus on organic farming, whereas this

research produced organic liquid fertilizer to deliver essential

nutrients for water spinach. Since our organic liquid fertilizers

are concentrated in nitrogen, phosphorus, and potassium when

growing, water spinach requires specific levels of the said

elements to have a high-quality harvest. There is no ideal season

for water spinach here in the Philippines; thus, the experiment

started in March and ended in May 2021. The researchers will use

only garden soil in all setups to have uniformity among them. All

plants were watered simultaneously during the experimentation.

Additionally, the organic liquid fertilizers were also

administered on experimental setups at the same time. Once the

water spinaches reach the harvest time, the experiment will end.

For the scope of the study, plant growth factors such as

required season, water, sunlight, and nutrients are only

considered in this study. Other variables that can affect the

growth of the water spinaches, such as temperature, weather,

humidity, and soil acidity, are not considered in this study.

These variables are not considered since the plant subjects'

environment is nonmanipulable, meaning the researchers cannot

control the said variables for an experimental setup. Changes in


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temperature, weather, and humidity will occur naturally, while

the researchers do not measure the soil acidity during the

experiment.

Furthermore, the researchers will ferment the ingredients

that will be used on each organic fertilizer. However, this study

will not scientifically determine how the nutrients within the

ingredients were collected. In addition, the researchers will

only focus on the specific nutrient content of the ingredients,

nitrogen for pumpkin seeds, phosphorus for green beans, and

potassium for baked potatoes. Other nutrients and minerals that

are present in each ingredient are disregarded.

Moreover, the gathered data in this research regarding plant

growth only included fresh leaf mass, dry leaf mass, number of

leaves, and final height. Other measurable data that can

determine plant growth aside from the mentioned variables are not

considered in this study.

Significance of the Study

This research will be beneficial to the following:

Agricultural Businesses. Plant-based agricultural

businesses will benefit from this study since the study is all

about organic farming. Any businesses that are interested in

transferring to organic agriculture can gather important


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information about it. Additionally, the knowledge about creating

organic liquid fertilizers would also be helpful to their field.

Gardeners. The study will help them take care of the

plant within their vicinity as the organic liquid fertilizer will

be able to sufficiently give the plant the nutrients it needs. It

will also give the idea towards the gardener that they can

harvest the food out of their very own garden hence easing the

monetary pressure when it comes to buying food at the local

supermarket. Due to the pandemic many people are looking for them

to burn time throughout the pandemic lockdown, and they found

their way in planting. The study will surely give ideas to the

local gardeners on how to effectively plant water spinach.

Farmers. The concentrated organic liquid fertilizers can

help the farmers provide the essential nutrients for the plant

growth of their various crops. In addition, this study provided

information about organic fertilizer creation, which can guide

farmers on making their organic fertilizer so that they will not

buy anymore from the market, resulting in lesser expenses.

Consumers. Fertilizers conclude a substantial part in

furnishing crops with the supplements they need to develop and

collect nutritious food. It helps convey sufficient food to take

care of the whole people. However, excessive use of chemical

fertilizers on plants can affect the health of the consumers. It


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may lead to respiratory problems, cardiac diseases, and various

types of cancers. Chemical fertilizers also contribute to various

diseases such as the West Nile virus, malaria, and cholera

(Kerkar, 2019). In contrast, organic fertilizers ensures that the

produced crops are free from harmful chemicals. Due to this

reason, consumers who eat organic products have lower risk to

diseases like cancer, stroke, and skin disorders (Patil, 2020).

Environment. The use of chemical fertilizers can harm the

environment. It can affect the waterways due to chemical runoffs

which can result to oxygen depletion that can kill fishes.

Additionally, chemical fertilizers also increase the air

pollution. Excess nitrogen from chemical fertilizers contributes

to the release of greenhouse gases such as carbon dioxide and

nitrous oxide into the atmosphere. This effect is caused by using

massive amounts of chemical fertilizer than the plants can

readily absorb. Lastly, uncontrolled use of chemical fertilizers

can cause solid acidification due to decreased organic matter in

the soil (Chandini, Kumar, Kumar, & Prakash, 2019). In

comparison, organic fertilizers are much safer for the

environment since they are made out of organic materials that are

biodegradable and does not affect environmental pollution (Patil,

2020). Hence, the organic liquid fertilizers are more suitable

for the environment compared to chemical fertilizers.


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Students. They can expand their knowledge in the field of

science. This study can also help students to produce their

alternative solution for improving the development of the plant.

Science Teachers. Since young generations have

inquisitive minds, they need suitable mentors that can fulfill

their curiosity. In this study, science teachers can derive

experiments on making organic liquid fertilizers by methods used

in this study. Through experimentation, the knowledge of students

that are eager to learn about science and plants can expand. In a

way that they can produce their study or research about plants or

fertilizer in future. Furthermore, the outcomes of this research

paper can serve as valuable information regarding agricultural

science. Wherein teachers can use them as lessons to teach their

students regarding planting crops and organic fertilizer

creation.

Future Researchers. This research paper can be use as

relevant information to support other research papers from the

near future, it does not only benefit to future researchers it

can also benefit to those people who have their own garden in

their yard they can use this research paper to have an idea on

how to make an Organic Liquid Fertilizer, Future researchers

would also be able to check ideas or claims that proves other

research statements.
21

Definition of Terms

The following terms have been used operationally in this

study:

K-based. A potassium rich organic liquid fertilizer.

N-based. An organic liquid fertilizer that is

concentrated with nitrogen.

No Fertilizer Setup. It is a group of water spinach that

were not subjected to any organic fertilizer; hence they only

receive water-only treatment.

Organic Liquid Fertilizer. It is an experiental solution

used in the study to shower plants with essential nutrients to

improve their quality and growth.

P-based. A phosphorus concentrated organic liquid

fertilizer.

Water Spinach. It is the subject plant in the study that

experiences the three concentrated organic liquid fertilizers and

no fertilizer treatment.
22

CHAPTER II

REVIEW OF RELATED LITERATURE AND STUDIES

This chapter contains the literatures and studies which are

used as the foundation of this research paper.

Related Literature

Water spinach, or kangkong, is an edible plant that is

common in Southeast Asia. It has a nutty flavor that is similar

to spinach. The day before planting, the water spinach seeds

should be soaked in water to start the germination process. The

seeds must be placed in a shallow basin with a thin covering of

water. Then, plant the seeds inside trays after the germination

period. Dig 1 to 2 inches in each hole and cover the seeds with

soil. The try should reach the minimum requirement of depth 2 to

3 inches to allow the plant to develop its roots. Once the

seedling reaches 4 to 6 inches, it should be transferred to a

larger container. The water spinaches should be at least 6 inches

apart when transplanted (Kurtz, 2021). The container must have a

depth of 12 inches to allow the roots to grow.

Furthermore, water spinach can grow well in either full or

partial sun. The plant should have at least 4 hours of sunlight

daily for the development of its leaves. Daily watering is

required to keep the moisture of the soil (Handy, 2020). After

three weeks, the water spinach is ready for harvest since water
23

spinach is one of the high-yielding plants (East-West Seed,

2021). The ideal season for water spinach in the Philippines is

year-round since the plant can grow anytime in a tropical climate

(Balcony Garden Web, n.d.).

The various articles are essential for the researchers

because of numerous valuable information about growing the

subject plant, water spinach. One of the critical pieces of

information in the articles is the container's required size for

growing water spinach. This learning will ensure that the

researchers will use the right amount of soil to grow the roots

of the water spinach. It also indicates the plants' necessary

spacing to provide enough room for the water spinaches to reach

their maximum size and avoid suffocating them from such

nutrients. Additionally, the researchers will follow the harvest

time provided by the articles, which is three weeks or twenty-one

days.

Nitrogen, phosphorus, and potassium are essential for

healthy plant growth since they are the macronutrients for

plants, and each performs a different role. Nitrogen is

fundamental for plants to create proteins that are significant

for the development of cells inside the plant. As such, nitrogen

is needed for robust plant growth, speedy development of shoots,

healthy flower bud development, and a good quality harvest. It is


24

also a fundamental chemical in the photosynthesis cycle, by which

plants convert sunlight into useable energy. In comparison,

phosphorus plays several critical roles in physical plant

development. Firstly, it is used by the plant to transport

nutrients and energy around itself so that all parts of the plant

will remain healthy. With nitrogen, it helps in the process of

photosynthesis, and it is also a crucial component in the

formation of nucleic acids, which help form the plant’s DNA, and

so helps plants grow strong and develop solid roots. Lastly,

potassium is vital in guaranteeing all the physiological

processes in a plant function normally. It is a component that

assists the plant in activating enzymes, form sugars, and

synthesize proteins. Potassium exists in two forms in the soil,

one soluble and the other not. Plants can only use the soluble

form of potassium, as it functions within the stomata, the cell

system within the plant that uses water to cycle nutrients around

all parts of the plant. Good levels of potassium help the plant

use moisture efficiently, which helps prevent disease and heat

damage and reduce the need for the plant to be irrigated

(Regenerative, 2014).

Considering nitrogen, phosphorus and potassium are essential

elements for the plants' growth and development. The researchers

selected various ingredients of each component to develop an

effective organic liquid fertilizer. Green beans are picked for


25

the phosphorus component as it is a popular source of protein and

it contains at least 250 mg of phosphorus per cup (Julson, 2018).

As for the potassium component, the researchers will use baked

potato as the main ingredient as it contains the highest

potassium content per single potato with 925 mg (Healthwise

Staff, 2020). Furthermore, this study's outcome is highly based

on observation regarding the effects of organic liquid fertilizer

on the lettuce and its growth and development.

Moreover, the researchers used molasses in order to ferment

the ingredients of each component. The amount of molasses should

be equal to the amount of the organic material. Furthermore, the

fermentation process of the plant materials must be at least

seven days to extract the nutrients within the various

ingredients. The containers must be on a shaded area to ensure

the success of the fermentation (Davenport, 2019). Seeing this

information, the researchers will ferment the plant ingredients

within seven days or one week to successfully produce the

concentrated organic liquid fertilizers.

The weather plays an enormous part in the plant's growth.

Most field crops are reliant on the weather to provide life‐

sustaining water and energy. It can result in production

casualty, particularly whenever it is during the ideal season. El

Niño gives rise to water deficiencies, postpones planting


26

seasons, and for the worse part, decreases crop yields. The

organic liquid fertilizer would be helpful especially in farmers

during the drought season. According to an article named "Organic

fertilizer to rehab Iloilo's El Niño-hit farms" by Gail Momblan

(2019), Iloilo Provincial Office (PAO) said it is peering toward

utilizing fluid, natural manures to restore rice ranches hit by

the new delayed drought or El Niño. Fertilizer can help the

plant's growth as well as it can increase the soil water

availability.

The researchers want to produce organic liquid fertilizer

that is manageable to make without consuming money and distribute

healthy agricultural products to the community. Also, our farmers

can produce additional products if they conduct their crops with

organic liquid fertilizer, making their farms more efficient.

Moreover, water spinach requires 15% nitrogen, 15%

phosphorus, and 15% potassium on its fertilizer since it requires

an NPK level of 15-15-15. Additionally, the fertilizer must be

administered on the first and second week of planting, 100 gfor

the first week and 50 g for the second (East-West Seed, 2018).

For these reasons, the researchers will produce the N-based, P-

based, and K-based organic liquid fertilizers with a

concentration of 15%. Hence, the solution will be 85% water, and

the remaining 15% will be the concentrated organic liquid


27

fertilizer. Furthermore, the researchers will rely on the

information provided by the article on when the organic liquid

fertilizers should be administered which is first and second week

of planting.

Related Studies

Previous research has developed significant ways of

determining results on experiments in which water spinach is the

subject. In the study entitled “The Effects of Spent Mushrooms

Compost and Various Composting Starter Combination on the Growth

and Yield of Kangkong” (2019) by researchers Mardiyani, Murwani,

and Lestari from the University of Islam Malang, Indonesia, the

study observed the following data: final height of plants, number

of leaves, fresh leaf mass per plant, and dry leaf mass per

plant. The final heights of the plants are gathered before the

plants are harvested; the measurement is from the ground up to

the highest point of the plant. While number of leaves is

collected by counting the number of leaves of kangkong. The fresh

leaf mass is weighed when the kangkong subjects are harvested. It

implies the weight of the plant right after the harvest or the

amount of plant material with water content. On the other hand,

dry leaf mass is collected by dehydrating the plants and weighing

them after. It implies the amount of plant material of the plant

without water or the constant mass of the plant.


28

Considering the study of Mardiyani, Murwani, and Lestari,

the researchers will follow the necessary data that they applied

to their research which are fresh leaf mass, dry leaf mass, final

height, and the number of leaves. Moreover, the researchers

envision that the following data will provide vital information

to determine the significant differences between the four setups.

Moving on, the researchers select pumpkin seed as the main

ingredient of nitrogen concentrated organic liquid fertilizer.

Considering it possesses a high nitrogen content of 5.53%

(Asaduzzaman et al., 2015).

While the usage of the sunlight in getting the dry weight is

done in the study of the researchers Abas, Ismail, Khatib, and

Sajak entitled Effects of Different Drying Treatments and Solvent

Ratios on Phytochemical Constituents of Ipomoea Aquatica and

Correlation with -Glucosidase Inhibitory Activity (2016). Other

methods are complicated with more usage of chemical composition

and complex mathematical formulas. However, the most traditional

way is by drying in the sun. The samples were arranged in a

drying rank and exposed to the sun for ten days. The researchers

ensured the complete dryness of the subject before further

analysis. The dried water spinaches are then grounded into a fine

powder and then sent to a laboratory to check the total dry

weight.
29

Organic fertilizer gives the plants nutrients in which they

cannot regularly get from plain soil. Plants react to the climate

surrounding them. Some may not fully grow because of the climate,

while others may take advantage of the climate to fully mature.

According to a study conducted in 2017, "Validating the

organic production systems for lettuce in Camarines Sur,

Philippines." Despite the difficulties associated with limited

science-based technology, shortage of organic inputs, high labor

costs, and farmers' reluctance to break the chemical-based

farming habit, organic agriculture can still provide

comprehensive and long-term solutions to food security issues,

health, and environmental protection (Cervantes, Laynesa, &

Pacis, 2017).

The study of Cervantes, Laynesa, and Pacis is closely

related to this research paper because they both have the same

goal. Their research and the researchers' study aim to promote

organic farming to lessen the use of chemical-based products in

agriculture. The said study gives sufficient evidence that

organic agriculture would work in the Philippines due to its

sustainability. Even if organic agriculture still has flaws, it

can still provide a better agricultural system that anyone can

benefit from. Thus, the researchers would use this study as proof

that practicing organic farming in the Philippines is feasible.


30

CHAPTER III

RESEARCH DESIGN AND METHODOLOGY

This chapter discusses the methods of research employed, the

subjects and locale of the study, the sampling techniques and

statistical tool used, and the data gathering procedure.

Methods of Research

This study is an experimental research. The researchers aim

to conduct an experiment and compare the results. The researchers

found out that pumpkin seeds, beans, and baked potato are good

ingredients in creating organic liquid fertilizers. These

ingredients undergo fermentation to produce concentrated organic

liquid fertilizers. The pumpkin seeds for N-based organic

fertilizer, beans for P-based organic fertilizer, and baked

potato for potassium K-based organic fertilizer. The researchers

applied the methodology used from previous studies to evaluate

the significant differences regarding the plant’s growth, which


31

are measuring the final height of the plant, counting the number

of leaves, and weighing the fresh leaf mass and the dry leaf mass

of the harvested water spinach. By applying the concentrated

organic liquid fertilizers to the water spinaches, the

researchers expect that each plant’s growth will differentiate

from one another.

Subject of the Study

The subjects of the study are the water spinaches that

receive organic liquid fertilizers during their growth, such as

the three setups, namely N-based, P-based, and K-based organic

fertilizers. Additionally, the other water spinaches that

experience the no fertilizer treatment are also subjects of the

study. Water spinach (Ipomoea aquatica), or kangkong, is an

edible plant that is common in the Southeast Asia region. Due to

the tropical climate of the Philippines, the said plant has no

specific ideal season since it can grow all year. And the harvest

time of the plant is three weeks or twenty-one days. The

researchers acquired the water spinach seeds from a supermarket

at Mayamot, Antipolo City. Furthermore, fresh leaf mass, dry

leaf mass, number of leaves, and the final height of the water

spinaches will be assessed through series of measurements when

the experiment concluded.


32

Locale of the Study

The researchers carry out the experimentation in the house

of one of the researchers in St. Oaks Residences, located in

Barangay San Isidro, Cainta, Rizal, near La Colina Subdivision

and Xentro Mall Antipolo. The stated house can accommodate all of

the pots and containers used in planting and growing water

spinaches in this study. Moreover, the researchers also conduct

the creation of the three organic liquid fertilizers and the one-

week fermentation process in the same house. However, due to

personal reasons, the water spinaches were transported to the

province of the selected researcher to finish the experimentation

process. The new location of the study is Purok 3, Barangay

Sampaloc, Pantabangan, Nueva Ecija. The nearest landmark on the

said location is the barangay hall of Sampaloc. The house in the

new location also facilitates gathering the needed statistical

data in the study's experiment, such as the fresh leaf mass, dry

leaf mass, number of leaves, and final plant's height.

Research Instruments

For this experimental study, the researchers created a data

sheet to record statistical data after the experimentation.

Additionally, there are several things to be considered as the

study focuses on the organic liquid fertilizers' effects to

improve water spinach growth, such as fresh leaf mass, dry leaf
33

mass, number of leaves, and final plant's height. When the

researchers obtain these various measurements, the researchers

will log them into the said data sheet for documentation. All

data are closely recorded to avoid any error in the study.

Moreover, the data sheet promotes the collocation of

observations, analysis, and measured data, which is highly

important for determining significant differences and

establishing precise results.

Data Gathering Procedure

In order to gather all necessary data for the study, the

researchers were guided by the following steps and procedures:

1. Prior to the day of planting, the water spinach seeds were

soaked in water on a basin to start the germination process.

2. The seeds are then transferred to eggs tray with a depth of

two inches to allow the plant roots to grow. The researcher

dig one inches to place the seeds and after that they were

covered in soil.

3. The water spinaches are watered daily to maintain the

moisture of the soil. And the researchers ensure that the

plant will receive at least 4 hours of sunlight.

4. When the seedlings reach the height of 4 inches, they were

transferred to large containers that have a depth of 12


34

inches. The seedlings are 6 inches apart when they were

planted for them to have sufficient space to grow.

5. The researchers conducted fermentation of the ingredients of

each concentrated organic liquid fertilizer.

a) The first step is adding the main ingredient to the

mixture and molasses with the same masses.

a. The researchers selected pumpkin seeds as the main

ingredient for the N-based fertilizer since it has

a high nitrogen content.

b. For the P-based fertilizer, the researchers picked

green beans as the main ingredient since it is a

popular source of protein and has a high content

of phosphorus.

c. The researchers will use baked potato as the main

ingredient for the K-based fertilizer since it

contains the highest potassium content per single

potato.

b) The three (3) organic liquid fertilizers are assigned

in separate containers and left in a dim room for 7

days. Then, the mixtures are filtered to remove a solid

particle from the liquid fertilizer.

6. There would be four (4) setups of plants: no fertilizer, N-

based organic liquid fertilizer, P-based organic liquid


35

fertilizer, and K-based organic liquid fertilizers. Each of

these setups has ten (10) plants to be monitored.

7. For the no fertilizer setup, the plants are watered daily to

maintain the moisture of the soil that allows the plant to

efficiently collect nutrients.

8. The N-based, P-based, and K-based organic liquid fertilizers

are administered on the first and second week of planting.

Specifically, during 100 g of fertilizer on the seventh day

and 50 g fourteenth day of planting.

9. On the third week, the water spinaches are harvested.

10. When the water spinaches were harvested, the

researchers count the sprouted leaves of each water spinach

to collect the number of leaves.

11. Afterward, the researchers get the water final height

of water spinaches by measuring height of the water spinach

from the ground to leaves in centimeters (cm ) using a

measuring tape.

12. The fresh mass is also determined right after the water

spinaches were harvested. It is measured in grams ( g) using

a electronic weighing balance to ensure accurate

measurements.

13. On the other hand, the dry mass is achieved after the

harvested water spinaches are dehydrated and weighed in


36

grams ( g) using a weighing balance. The researchers used a

dried the water spinach subjects under the sun for 10 days

to ensure that the subjects are completely dehydrated.

14. Once all of the required data are collected, the

researchers will interpret the recorded measurements.

Treatment of Data

The study needed the following statistical tools to treat

the data gathered.

A. Mean

Σ xi
x=
n

Where:

x i = data

n = number of data points

This statistical tool is used to obtain the

computational average of the control and the three

concentrated organic liquid fertilizers.


37

B. Standard Deviation


2
Σ( x i−x)
s=
n−1

Where:

x i = data

x = mean

n = number of data points

This statistical tool is applied to know the distance

between the mean and each data points of this study.

C. Analysis of Variance (ANOVA)

1. Correction Factor
2
(Σ ¿ ¿ i Σ j x ij )
C= ¿
n

2. Total Sum of Squares (TSS)


2
TSS=Σ i Σ j ( xij ) −C

3. Sum of Squares among Groups (SSAG)

SSAG= Σ i
( (Σ j xij )2
ni )
4. Sum of Squares within Groups (SSWG)

SSWG=TSS−SSAG

5. Degree of Freedom

a) df among=i−1

b) df total =n−1
38

c) df within=n−i

6. Mean Square (MS)

SSAG
MS among =
df among

SSWG
MS within=
df within

7. Standard Deviation within Groups

SD within= √ MS within

8. F-Ratio

MS among
F=
MS within

This statistical tool was used in order to know the

significant differences between the control and the three

concentrated organic fertilizers in the study.

D. T-test (One-tailed)

t=¿ x 1−x 2∨ ¿ ¿


2 2
s s2
1
+
n1 n 2

Where:

x 1 - mean of the 1st group of data

x 2 - mean of the 2nd group of data

2
s1 – standard deviation of the 1st group of data

s22 - standard deviation of the 2nd group of data

n1 – sample size of the first group of data


39

n2 - sample size of the first group of data

This statistical tool was used in order to know which

setups performed better:

a) N-based organic liquid fertilizer and no fertilizer

b) P-based organic liquid fertilizer and no fertilizer

c) K-based organic liquid fertilizer and no fertilizer

d) N-based and P-based organic liquid fertilizers

e) N-based and K-based organic liquid fertilizers

f) P-based and K-based organic liquid fertilizers

Chapter IV

PRESENTATION, INTERPRETATION, AND ANALYSIS OF DATA

This chapter shows the gathered data which were tabulated

and interpreted for better understanding.

The researchers measure the necessary data for the study

such as total fresh leaf mass, total dry leaf mass, number of

leaves, and final height when all of the forty (40) water spinach

subjects are harvested.

Problem # 1: What is the average and standard deviation of each

of the four (4) setups in terms of the following:

a) Fresh leaf mass

No fertilizer N-based P-based K-based


x 3.2118 5.6995 5.0446 4.401
s 1.01976 0.88088 1.445 1.04866
Table 4.1. The result of total fresh leaf mass of water spinaches.
40

On the Table 4.1, the fresh leaf mass of water spinach

subjects that were administered with water-only and the three

organic liquid fertilizers has a range of 1 g to 7 g. The

water-only setup has the lowest average fresh leaf mass, while

the N-based setup has the highest. Additionally, the other two

concentrated organic fertilizer setups also exceed the average

fresh leaf mass of the water-only setup. This implies that the

three organic liquid fertilizers increased the fresh leaf mass

of the water spinach subjects.

Moreover, the standard deviation of water-only setup, P-

based, and K-based fertilizer are higher than one (1) which

means the distances of their data points and their mean are

far. On the other hand, the distance between the N-based data

points and its mean is close to each other since its standard

deviation is less than one (1).

b) Dry leaf mass

No fertilizer N-based P-based K-based


x 1.9002 3.0423 2.9435 2.6459
s 0.70046 0.82822 0.64254 0.77699
Table 4.2. The result of total dry leaf mass of water spinaches.

As seen in the Table 4.2, the dry leaf mass of water

spinach subjects that were applied with water-only and

concentrated organic liquid fertilizer has a range of 1 g to 4

g. The N-based setup has the highest average fresh leaf mass,
41

while the water-only setup has the lowest. As well as the

other two concentrated organic fertilizer setups also surpass

the average dry leaf mass of the water-only setup. This means

that the three organic liquid fertilizers improved the dry

leaf mass of the water spinach subjects. Furthermore, the

standard deviation of the four (4) setups is lower than one

(1) which means that the data points of each setup are close

to the mean.

c) Number of leaves

No fertilizer N-based P-based K-based


x 7.7 8.4 8.6 8.2
s 0.94868 0.69921 0.96609 1.03280
Table 4.3. The result of number of leaves of water spinaches.

In Table 4.3, the number of leaves of water spinaches that

were subjected to water-only treatment and three concentrated

organic liquid fertilizers vary from 6 to 10 leaves. The P-

based setup has the highest average fresh leaf mass, while the

water-only setup has the lowest. The other two concentrated

organic fertilizer setups also excel the average number of

leaves of the water-only setup. Which means that the three

concentrated organic liquid fertilizers can increase the

number of leaves of the water spinach subjects.


42

In addition, the standard deviations of the setups are lower

than one (1) which means that the data points of each setup

are close to the mean, except the K-based setup which has a

greater than one (1) standard deviation.

d) Final Height

(cm) No fertilizer N-based P-based K-based


x 165.1 195.6 228.5 217.1
s 39.61888 40.08103 44.71950 25.82613
Table 4.4. The result of final height of water spinaches.

As can be observed in the Table 4.4, the final heights of

water spinaches that were subjected to water-only treatment

and three concentrated organic liquid fertilizers has a range

of 107 cm to 289 cm . The water-only setup has the lowest

average fresh leaf mass, while P-based setup has the highest.

The other two concentrated organic fertilizer setups also have

a higher average final height than the water-only setup. Which

means that the three concentrated organic liquid fertilizers

can improve the final height of the water spinach subjects.

Moving on, the standard deviations of the four (4) setups are

higher than one (1), this means that the data points on the

four setups are far from their mean.

Problem # 2: Is there a significant difference between the fresh

leaf masses of the four (4) setups?

ANOVA
43

Source of
SS df MS F P-value F crit
Variation
Between
33.72805 3 11.24268 8.98775 0.000141 2.866266
Groups
Within
345.03203 36 1.25089
Groups
Total 78.76008 39
Table 4.5. The result of analysis of variance in fresh leaf mass.

The researchers used analysis of variance (ANOVA) in order

to get the significant difference between the water-only setup

and the three concentrated organic liquid fertilizers. If the

computed F value is greater than the F critical value, then the

null hypothesis is rejected, otherwise accept null hypothesis.

Reject Ho: There is a significant difference between the three

concentrated organic liquid fertilizer and the control in terms

of fresh leaf mass.

As observed in table 4.5 that the F critical value is less

than the F , hence the null hypothesis is rejected.

Since the null hypothesis is rejected, the researchers

proceed in calculating for the one-tailed t-test to determine

which setup is better from other. The table below provides the

results in each pair of groups.

Groups t CV Findings Interpretations


N-based and
5.301057 1.833113 t > CV Reject HO
No fertilizer
P-based and
3.280761 1.833113 t > CV Reject HO
No fertilizer
44

K-based and
2.977871 1.833113 t > CV Reject HO
No fertilizer
N-based and
1.018977 1.833113 t < CV Fail to reject HO
P-based
N-based and
3.018737 1.833113 t > CV Reject HO
K-based
P-based and
0.892571 1.833113 t < CV Fail to reject HO
K-based
Table 4.6. The result of one-tailed t -test in fresh leaf mass.

Based on Table 4.6, all three concentrated organic liquid

fertilizers are better than the no fertilizer setup in terms of

fresh leaf mass. While the best performing fertilizer among the

setups is the N-based organic liquid fertilizer. Even if N-based

and P-based is equal, the N-based fertilizer is set to be the

best since P-based and K-based are equal, but the N-based is

better than the K-based.

The researchers expect this outcome since a study entitled

“Effects of Nitrogen Rates on Growth and Quality of Water Spinach

(Ipomea Aquatica)” (2018) by Ibrahim, Rahman, and Zain proved

that N-based fertilizers increase the biomass production of water

spinach. However, their study also showed that nitrogen

fertilizers do not improve the plant’s water absorption.

Considering that the fresh leaf mass of the water spinach

consists of plant material and water, the result of this study

slightly contradicts the outcome of the previous study.

Problem # 3: Is there a significant difference between the dry

leaf masses of the four (4) setups?


45

ANOVA
Source of
SS df MS F P-value F crit
Variation
Between
8.01099 3 2.67033 4.812869 0.006416 2.866266
Groups
Within
19.97392 36 0.554831
Groups
Total 34.975 39
Table 4.7. The result of analysis of variance in dry leaf mass.

Reject Ho: There is a significant difference between the three

concentrated organic liquid fertilizer and the control in terms

of dry leaf mass.

On table 4.7 that the F critical value is less than the F ,

thus the null hypothesis is rejected.

Since the null hypothesis is rejected, the researchers

proceed in calculating for the one-tailed t-test to determine

which setup is better from other. The table below provides the

results in each pair of groups.

Groups t CV Findings Interpretation


N-based and
2.918817 1.833113 t > CV Reject HO
No fertilizer
P-based and
2.816002 1.833113 t > CV Reject HO
No fertilizer
K-based and
2.501541 1.833113 t > CV Reject HO
No fertilizer
N-based and
0.318711 1.833113 t < CV Fail to reject HO
P-based
N-based and
1.089353 1.833113 t < CV Fail to reject HO
K-based
P-based and
0.824213 1.833113 t < CV Fail to reject HO
K-based
Table 4.8. The result of one-tailed t -test in dry leaf mass.
46

In Table 4.8, the result has shown that the three

concentrated organic liquid fertilizers are significantly better

than the no fertilizer setup in terms of dry leaf mass. However,

all organic fertilizers performed equally.

The researchers expected this outcome because the study

entitled “Effects of Nitrogen Rates on Growth and Quality of

Water Spinach (Ipomea Aquatica)” (2018) by Ibrahim, Rahman, and

Zain showed that N-based fertilizers increase the biomass

production of water spinach. In their study, the biomass of the

control or the no fertilizer setup is significantly lower than

the water spinaches that were subjected to nitrogen fertilizer.

Hence, the result of the experiment accepts the outcome of the

mentioned study.

In addition, potassium fertilizers are also expected to

increase the constant mass of the water spinach since, based on

the study of Linnemann, Hitipeuw, and Westphal entitled Influence

of potassium fertilizer on yield of upland kangkong (Ipomoea

aquatica Forsk.), potassium fertilizers increase the content of

plant material in stems and leaves of water spinach.

Problem # 4: Is there a significant difference between the number

of leaves of the four (4) setups?

ANOVA
Source of
SS df MS F P-value F crit
Variation
47

Between
4.475 3 1.491667 1.760656 0.172124 2.866266
Groups
Within
30.5 36 0.847222
Groups
Total 34.975 39
Table 4.9. The result of analysis of variance in number of leaves.

Accept Ho: There is no significant difference between the three

concentrated organic liquid fertilizer and the control in terms

of number of leaves.

On Table 4.9, the F value is less than the value of F

critical, thus the null hypothesis is accepted. As a result,

there are no significant differences between the four setups. It

can also be observed in the Table 4.3 that the average number of

leaves of each setup are close to each other.

The researchers do not expect this outcome since, based on a

previous study, N-based fertilizers can increase the leaf

production of water spinach (Ibrahim, Rahman, & Zain, 2018). The

study’s result showed that N-based fertilizers performed better

than the no fertilizer setup. The outcome of the previous study

contradicts the result of the experiment. Wherein the no

fertilizer setup and the N-based fertilizer performed equally.

Thus, the outcome of the experiment contradicts the previous

study’s result.

Problem # 5: Is there a significant difference between the final

heights of the four (4) setups?


48

ANOVA
Source of
SS df MS F P-value F crit
Variation
Between
23321.08 3 7773.692 5.321743 0.003865 2.866266
Groups
Within
52586.7 36 1460.742
Groups
Total 75907.78 39
Table 4.10. The result of analysis of variance in final height.

Reject Ho: There is a significant difference between the three

concentrated organic liquid fertilizer and the control in terms

of final height.

It can be seen in table 4.10 that the F critical value is

less than the F , hence the null hypothesis is rejected.

Since the null hypothesis is rejected, the researchers

proceed in computing for the one-tailed t-test to determine which

setup is better from other. The table below provides the results

in each pair of groups.

Groups t CV Findings Interpretation


N-based and
1.61432 1.833113 t < CV Fail to reject HO
No fertilizer
P-based and
3.02636 1.833113 t > CV Reject HO
No fertilizer
K-based and
3.90415 1.833113 t > CV Reject HO
No fertilizer
N-based and
0.318711 1.833113 t < CV Fail to reject HO
P-based
N-based and
1.303876 1.833113 t < CV Fail to reject HO
K-based
P-based and
1.412339 1.833113 t < CV Fail to reject HO
K-based
Table 4.11. The result of one-tailed t -test in final height.
49

In Table 4.11, the result has shown that only two

concentrated organic fertilizers (P-based and K-based) performed

better than the no fertilizer setup in terms of final height.

Additionally, the three organic fertilizers performed equally.

However, the best performing organic fertilizers are P-based and

N-based. The researchers' interpretation is based on the fact

that despite the fertilizers have equal performances; still, the

N-based organic fertilizers do not surpass the results of the no

fertilizer setup.

The result in the final height of the water spinaches is

unexpected for the researchers since based on the study of

Ibrahim, Rahman, and Zain entitled Effects of Nitrogen Rates on

Growth and Quality of Water Spinach (Ipomea Aquatica) (2018), the

result of their study showed that N-based fertilizers

significantly improved the plant height of water spinaches

compared to water spinaches that were not administered with any

nitrogen fertilizer. In contrast, the result of this study's

experimentation showed that the result of K-based organic liquid

fertilizer and the no fertilizer setup on final height are equal

(Linnemann, Hitipeuw, & Westphal, 1986). Hence, the outcome of

the study contradicts the study of Ibrahim, Rahman, and Zain but

accepts the result of the study of Linnemann, Hitipeuw, and

Westphal.
50

Chapter V

SUMMARY, CONCLUSIONS, AND RECOMMENDATIONS

This chapter presents the summary of findings, conclusions,

and recommendations from the analysis mentioned in the previous

chapter.

Summary

Since this is an experimental research, the researchers must

gather all the necessary data by using data sheet. The

researchers want to promote the use of organic fertilizers to


51

lessen the usage of chemical fertilizers. Hence, the researchers

found out the essential nutrients for plant growth, such as

nitrogen, phosphorus, and potassium. The researchers also

searched for plants that are rich in the said nutrients to

produce concentrated organic liquid fertilizers. The ingredients

used in each component are pumpkin seeds for N-based, green beans

for P-based, and baked potato for K-based. After the three

concentrated organic fertilizers are made, they were subjected to

water spinach subjects. When the subject plants were harvested,

the researchers collected the necessary data to determine the

significant differences of each setup. The said necessary data

are fresh mass, dry mass, number of leaves, and final height of

the plant. In order for the researchers to interpret the gathered

data, the researchers used mean (x ), analysis of variance (F), and

one-tailed t -test (t) .

Here is the summary of each result:

1. The results in calculating the average and standard

deviation of the four setups are the following:

a) The N-based setup has the highest mean in terms of

fresh leaf mass, while the no fertilizer setup has the

lowest mean. Furthermore, the average distance between

the data points and the mean of each component is

small.
52

b) The N-based setup has the highest average dry leaf

mass, while the no fertilizer setup has the lowest.

Additionally, the data points and the mean of each

component are close to one another.

c) The P-based setup has the highest mean in terms of

number of leaves, while the no fertilizer setup has the

lowest. In addition, the data points have a short

distance between the mean of each setup.

d) The N-based setup has the highest average final height,

while the no fertilizer setup has the lowest. Moreover,

the data points in each setup are widely scattered.

2. The no fertilizer setup and the concentrated organic liquid

fertilizers have a significant difference in terms of fresh

leaf mass. After comparing each setup, it was proven that

all organic fertilizers are significantly better than the no

fertilizer setup. Among the three organic fertilizers, the

N-based organic fertilizer is the best performing.

3. The no fertilizer setup and the concentrated organic liquid

fertilizers have a significant difference in dry leaf mass.

When each setup was compared, it was proven that all organic

fertilizers performed better than the result of no

fertilizer setup. At the same time, the performance of each

organic fertilizer is equal.


53

4. The no fertilizer setup and the concentrated organic liquid

fertilizers have no significant difference in number of

leaves, and this means all setups performed equally.

5. The no fertilizer setup and the concentrated organic liquid

fertilizers have a significant difference in terms of final

height. After comparing each setup, it was proven that

organic fertilizers are better than the no fertilizer setup

except the N-based. Additionally, the P-based and K-based

organic fertilizers have the best performance.

Conclusion

After the experiment, the researchers concluded the

following:

1. The conclusions for the first problem are the following:

a) In terms of fresh leaf mass, the mass of the water

spinach subjects ranges from 1 g to 7 g. In the

individual fresh leaf mass, the no fertilizer setup has

a range of 1 g to 5 g with an average of 3.212 g and a

standard deviation of 1.020, the N-based setup has a

range of 4 g to 7 g with an average of 5.560 g and a

standard deviation of 0.881, the P-based setup has a

range of 3 g to 7 g with an average of 5.0446 g and a

standard deviation of 1.445, and the K-based setup has


54

a range of 2 g to 5 g with an average of 4.401 g and a

standard deviation of 1.049.

b) In terms of dry leaf mass, the mass of the water

spinach subjects ranges from 1 g to 4 g. In the

individual fresh leaf mass, the no fertilizer setup has

a range of 1 g to 3 g with an average of 1.9002 g and a

standard deviation of 0.701, the N-based setup has a

range of 2 g to 4 g with an average of 3.042 g and a

standard deviation of 0.828, the P-based setup has a

range of 1 g to 4 g with an average of 2.944 g and a

standard deviation of 0.64254, and the K-based setup

has a range of 1 g to 3 g with an average of 2.646 g

and a standard deviation of 0.777.

c) In terms of number of leaves, the water spinaches have

6 to 10 leaves. In the individual number of leaves, the

no fertilizer setup has 6 to 9 with an average of 7.7

and a standard deviation of 0.949, the N-based setup

has 7 to 9 with an average of 8.4 and a standard

deviation of 0.699, the P-based setup has 8 to 10 with

an average of 8.6 and a standard deviation of 0.966,

and the K-based setup has 7 to 9 with an average of 8.2

and a standard deviation of 1.033.

d) As for the final height of the water spinaches, it

ranges from 107 cm to 289 cm . When it comes to


55

individual final height, the control varies from 107 cm

to 231 cm with an average of 165.1 cm and a standard

deviation of 39.619, the N-based varies from 147 cm to

270 cm with an average of 195.6 cm and a standard

deviation of 40.081, the P-based varies from 154 cm to

289 cm with an average of 228.2 cm and a standard

deviation of 39.619, and the K-based varies from 161 cm

to 247 cm with an average of 217.1 cm and a standard

deviation of 25.826.

2. In the significant difference between the no fertilizer

setup and the concentrated organic liquid fertilizers in

terms of fresh leaf mass, the F value is greater than the F

critical value. Therefore, the null hypothesis is rejected.

a) In the one-tailed t-test between the N-based organic

liquid fertilizer and the no fertilizer setup, the

result has shown that the t statistic is greater than

the t critical value. Therefore, the null hypothesis is

rejected; this means that the N-based is better than

the no fertilizer setup.

b) In the one-tailed t -test between the P-based organic

liquid fertilizer and the no fertilizer setup, the

result has shown that the t statistic is greater than

the t critical value. Therefore, the null hypothesis is


56

rejected; this means that the P-based is better than

the no fertilizer setup.

c) In the one-tailed t -test between the K-based organic

liquid fertilizer and the no fertilizer setup, the

result has shown that the t statistic is greater than

the t critical value. Therefore, the null hypothesis is

rejected; this means that the K-based is better than

the no fertilizer setup.

d) In the one-tailed t -test between the P-based organic

liquid fertilizer and the N-based, the result has shown

that the t statistic is less than the t critical value.

Hence, the null hypothesis is accepted; this means that

N-based and P-based organic liquid fertilizer are

equal.

e) In the one-tailed t -test between the N-based organic

liquid fertilizer and the K-based, the result has shown

that the t statistic is greater than the t critical

value. Therefore, the null hypothesis is rejected; this

means that the N-based is better than the K-based.

f) In the one-tailed t -test between the K-based organic

liquid fertilizer and the P-based, the result has shown

that the t statistic is less than the t critical value.

Hence, the null hypothesis is accepted; this means that


57

P-based and K-based organic liquid fertilizer are

equal.

3. In the significant difference between the no fertilizer

setup and the concentrated organic liquid fertilizers in

terms of dry leaf mass, the F value is greater than the F

critical value. Therefore, the null hypothesis is rejected.

a. In the one-tailed t -test between the N-based organic

liquid fertilizer and the no fertilizer setup, the

result has shown that the t statistic is greater than

the t critical value. Therefore, the null hypothesis

is rejected; this means that the N-based is better

than the no fertilizer setup.

b. In the one-tailed t -test between the P-based organic

liquid fertilizer and the no fertilizer setup, the

result shown that the t statistic is greater than the t

critical value. Therefore, the null hypothesis is

rejected; this means that the P-based is better than

the no fertilizer setup.

c. In the one-tailed t -test between the K-based organic

liquid fertilizer and the no fertilizer setup, the

result shown that the t statistic is greater than the t

critical value. Therefore, the null hypothesis is

rejected; this means that the K-based is better than

the no fertilizer setup.


58

d. In the one-tailed t -test between the P-based organic

liquid fertilizer and the N-based, the result has

shown that the t statistic is less than the t critical

value. Hence, the null hypothesis is accepted; this

means that N-based and P-based organic liquid

fertilizer are equal.

e. In the one-tailed t -test between the K-based organic

liquid fertilizer and the N-based, the result has

shown that the t statistic is less than the t critical

value. Hence, the null hypothesis is accepted; this

means that N-based and K-based organic liquid

fertilizer are equal.

f. In the one-tailed t -test between the K-based organic

liquid fertilizer and the P-based, the result has

shown that the t statistic is less than the t critical

value. Hence, the null hypothesis is accepted; this

means that P-based and K-based organic liquid

fertilizer are equal.

4. In the significant difference between the no fertilizer

setup and the concentrated organic liquid fertilizers in

terms of number of leaves, the F value is less than the


59

critical value of F. Hence, the null hypothesis is

accepted.

5. In the significant difference between the no fertilizer

setup and the concentrated organic liquid fertilizers in

terms of final height, the F value is greater than the F

critical value. Therefore, the null hypothesis is rejected.

a. In the one-tailed t -test between the N-based organic

liquid fertilizer and the no fertilizer setup, the

result has shown that the t statistic is less than the

t critical value. Hence, the null hypothesis is

accepted; this means that the no fertilizer setup and

N-based organic liquid fertilizer are equal.

b. In the one-tailed t -test between the P-based organic

liquid fertilizer and the no fertilizer setup, the

result has shown that the t statistic is greater than

the critical value of t . Therefore, the null hypothesis

is rejected; this means that the P-based is better than

the no fertilizer setup.

c. In the one-tailed t -test between the K-based organic

liquid fertilizer and the no fertilizer setup, the

result has shown that the t statistic is greater than


60

the t critical value. Therefore, the null hypothesis is

rejected; this means that the K-based is better than

the no fertilizer setup.

d. In the one-tailed t -test between P-based organic liquid

fertilizer and N-based, the result has shown that the t

statistic is less than the t critical value. Thus, the

null hypothesis is accepted; this means that the P-

based and N-based organic liquid fertilizers are equal.

e. In the one-tailed t -test between K-based organic liquid

fertilizer and N-based, the result has shown that the t

statistic is less than the t critical value. Thus, the

null hypothesis is accepted; this means that the K-

based and N-based organic liquid fertilizers are equal.

f. In the one-tailed t -test between P-based organic liquid

fertilizer and K-based, the result has shown that the t

statistic is less than the t critical value. Thus, the

null hypothesis is accepted; this means that the P-

based and K-based organic liquid fertilizers are equal.

Recommendations

1. The researchers would like to suggest the use of containers to

separate each setup orderly. It is also advisable to use a


61

measuring tape to gather the final height of the plant since

it is flexible and can accurately follow the plant’s height.

While the use of an electronic weighing scale in weighing the

fresh and dry mass of the plant is also recommended

considering it provides precise measurements.

2. The researchers recommend using sunlight to get the dry leaf

mass of the plant if dehydrators or ovens are not accessible

for future researchers.

3. Future researchers should find other literature and studies

regarding the effects of N-based, P-based, and K-based

fertilizers on water spinach since there is only a small

number of sources that are available on the internet,

primarily studies or articles regarding the influence of

phosphorus concentrated fertilizers on water spinach growth

does not exist on the internet. Finding studies related to P-

based fertilizers and water spinach is essential to a more

meaningful interpretation of the significant differences

between the setups.

4. Further studies focusing on different types of plants and

other ingredients on each component should be carried out.

Additionally, conducting studies with various combinations of

the three concentrated organic liquid fertilizers is also

recommended.
62

5. The researchers recommend that pumpkin seeds, green beans, and

baked potato can be used as an ingredient in producing organic

liquid fertilizers. The pumpkin seeds are for N-based, green

beans for P-based, and baked potato for K-based organic liquid

fertilizer.

6. The researchers would recommend creating and using organic

fertilizers when growing plants at home or even at farms. It

is also recommended to use any organic materials as long as

they are rich in essential nutrients.

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71
72

Appendix A
APPENDICES
(Data Sheet)
73

Water Spinach (No fertilizer)


Fresh Leaf Dry Leaf Number of Final
Subject
Mass ( g) Mass ( g) Leaves Height (cm )
1 5.028 3.354 9 223
2 4.742 2.916 9 231
3 2.739 1.405 8 178
4 2.694 1.831 7 137
5 3.502 1.359 7 174
6 1.983 1.442 8 143
7 3.196 2.313 8 165
8 3.358 1.437 8 167
9 2.893 1.506 7 126
10 1.983 1.439 6 107
Table 6.1. Gathered data on water-only water spinach subjects.

Water Spinach (N-based)


Fresh Leaf Dry Leaf Number of Final
Subject
Mass ( g) Mass ( g) Leaves Height (cm )
1 5.209 2.347 8 175
2 5.324 2.843 7 147
3 7.094 4.436 9 270
4 6.498 2.351 9 248
5 5.743 3.043 9 221
6 6.109 4.154 9 198
7 5.301 3.543 9 165
8 6.502 2.247 8 200
9 4.032 3.406 8 170
10 5.183 2.053 8 162
Table 6.2. Gathered data on N-based water spinach subjects.
74

Water Spinach (P-based)


Fresh Leaf Dry Leaf Number of Final
Subject
Mass ( g) Mass ( g) Leaves Height (cm )
1 4.210 2.403 9 211
2 6.324 2.553 10 250
3 3.094 4.143 8 167
4 4.293 3.350 8 154
5 6.593 3.546 7 215
6 7.031 2.684 10 289
7 5.330 2.503 8 248
8 3.094 3.148 8 212
9 6.183 1.963 9 274
10 4.294 3.142 9 265
Table 6.3. Gathered data on P-based water spinach subjects.

Water Spinach (K-based)


Fresh Leaf Dry Leaf Number of Final
Subject
Mass ( g) Mass ( g) Leaves Height (cm )
1 5.583 2.901 9 240
2 4.849 3.256 8 222
3 5.320 3.144 9 247
4 4.091 1.768 7 211
5 2.892 1.439 8 161
6 3.224 1.889 8 193
7 2.934 2.491 6 213
8 5.158 3.690 9 243
9 4.591 3.515 9 227
10 5.368 2.366 9 214
Table 6.4. Gathered data on K-based water spinach subjects.
75
76

Appendix B
(Solutions)
77

( g) No Fertilizer N-based P-based K-based


5.028 5.209 4.210 5.583
4.742 5.324 6.324 4.849
2.739 7.094 3.094 5.320
2.694 6.498 4.293 4.091
Total Fresh Leaf 3.502 5.743 6.593 2.892
Mass 1.983 6.109 7.031 3.224
3.196 5.301 5.330 2.934
3.358 6.502 3.094 5.158
2.893 4.032 6.183 4.591
1.983 5.183 4.294 5.368
x 3.2118 5.6995 5.0446 4.401
s 1.01976 0.88088 1.445 1.04866
Table 7.1. The result of total fresh leaf mass of water spinaches.

( g) No Fertilizer N-based P-based K-based


3.354 2.347 2.403 2.901
2.916 2.843 2.553 3.256
1.405 4.436 4.143 3.144
1.831 2.351 3.350 1.768
Total Dry Leaf 1.359 3.043 3.546 1.439
Mass 1.442 4.154 2.684 1.889
2.313 3.543 2.503 2.491
1.437 2.247 3.148 3.690
1.506 3.406 1.963 3.515
1.439 2.053 3.142 2.366
x 1.9002 3.0423 2.9435 2.6459
S 0.70046 0.82822 0.64254 0.77699
Table 7.2. The result of total dry leaf mass of water spinaches.
78

No Fertilizer N-based P-based K-based


9 8 9 9
9 7 10 8
8 9 8 9
7 9 8 7
Number of 7 9 7 8
Leaves 8 9 10 8
8 9 8 6
8 8 8 9
7 8 9 9
6 8 9 9
x 7.7 8.4 8.6 8.2
s 0.94868 0.69921 0.96609 1.03280
Table 7.3. The result of number of leaves of water spinaches.

(cm) No Fertilizer N-based P-based K-based


223 175 211 240
231 147 250 222
178 270 167 247
137 248 154 211
174 221 215 161
Final Height
143 198 289 193
165 165 248 213
167 200 212 243
126 170 274 227
107 162 265 214
x 165.1 195.6 228.5 217.1
s 39.61888 40.08103 44.71950 25.82613
Table 7.4. The result of final height of water spinaches.
79

Anova: Single Factor (Fresh leaf mass)


SUMMARY
Groups Count Sum Average Variance
No Fertilizer 10 32.118 3.2118 1.039903
N-Based 10 56.995 5.6995 0.775949
P-Based 10 50.446 5.0446 2.088016
K-Based 10 44.01 4.401 1.099692
ANOVA
Source of
SS df MS F P-value F crit
Variation
Between
33.72805 3 11.24268 8.98775 0.000141 2.866266
Groups
Within
345.03203 36 1.25089
Groups
Total 78.76008 39
Table 7.5. The result of analysis of variance in fresh leaf mass.

Anova: Single Factor (Dry leaf mass)


SUMMARY
Groups Count Sum Average Variance
No Fertilizer 10 19.002 1.9002 0.516811
N-Based 10 30.423 3.0423 0.685948
P-Based 10 29.435 2.9435 0.412851
K-Based 10 26.469 2.6459 0.603715
ANOVA
Source of
SS df MS F P-value F crit
Variation
Between
8.01099 3 2.67033 4.812869 0.006416 2.866266
Groups
Within
19.97392 36 0.554831
Groups
Total 34.975 39
Table 7.6. The result of analysis of variance in dry leaf mass.
80

Anova: Single Factor (Number of leaves)


SUMMARY
Groups Count Sum Average Variance
No Fertilizer 10 77 7.7 0.9
N-Based 10 84 8.4 0.488889
P-Based 10 86 8.6 0.933333
K-Based 10 82 8.2 1.066667
ANOVA
Source of
SS df MS F P-value F crit
Variation
Between
4.475 3 1.491667 1.760656 0.172124 2.866266
Groups
Within
30.5 36 0.847222
Groups
Total 34.975 39
Table 7.7. The result of analysis of variance in number of leaves.

Anova: Single Factor (Final Height)


SUMMARY
Groups Count Sum Average Variance
No Fertilizer 10 1651 165.1 1569.656
N-Based 10 1956 195.6 1606.489
P-Based 10 2285 228.5 1999.833
K-Based 10 2171 217.1 666.9889
ANOVA
Source of
SS df MS F P-value F crit
Variation
Between
23321.08 3 7773.692 5.321743 0.003865 2.866266
Groups
Within
52586.7 36 1460.742
Groups
Total 75907.78 39
Table 7.8. The result of analysis of variance in final height.
81

t -Test: Paired Two Samples for Means


N-based No fertilizer
Mean 5.6995 3.2118
Variance 0.775949 1.039903
Observation 10 10
Pearson Correlation -0.21509
Hypothesized Mean Difference 0
Df 9
t Stat 5.301057
P(T<=t) one-tail 0.000247
t Critical one-tail 1.833113
P(T<=t) two-tail 0.000493
t Critical two-tail 2.262157
Table 7.9. The one-tailed t -test result between N-based and no fertilizer in

terms of fresh leaf mass.

t -Test: Paired Two Samples for Means


P-based No fertilizer
Mean 5.0446 3.2118
Variance 2.088016 1.039903
Observation 10 10
Pearson Correlation 0.002379
Hypothesized Mean Difference 0
Df 9
t Stat 3.280761
P(T<=t) one-tail 0.004759
t Critical one-tail 1.833113
P(T<=t) two-tail 0.009518
t Critical two-tail 2.262157
Table 7.10. The one-tailed t -test result between P-based and no fertilizer in

terms of fresh leaf mass.


82

t -Test: Paired Two Samples for Means


K-based No fertilizer
Mean 4.401 3.2118
Variance 1.099692 1.039903
Observation 10 10
Pearson Correlation 0.254738
Hypothesized Mean Difference 0
Df 9
t Stat 2.977871
P(T<=t) one-tail 0.007752
t Critical one-tail 1.833113
P(T<=t) two-tail 0.015503
t Critical two-tail 2.262157
Table 7.11. The one-tailed t -test result between K-based and no fertilizer in

terms of fresh leaf mass.

t -Test: Paired Two Samples for Means


N-based P-based
Mean 5.6995 5.0446
Variance 0.775949 2.088016
Observation 10 10
Pearson Correlation -0.49758
Hypothesized Mean Difference 0
Df 9
t Stat 1.018977
P(T<=t) one-tail 0.167411
t Critical one-tail 1.833113
P(T<=t) two-tail 0.334822
t Critical two-tail 2.262157
Table 7.12. The one-tailed t -test result between N-based and P-based in terms

of fresh leaf mass.


83

t -Test: Paired Two Samples for Means


N-based K-based
Mean 5.6995 4.401
Variance 0.775949 1.099692
Observation 10 10
Pearson Correlation 0.013737
Hypothesized Mean Difference 0
Df 9
t Stat 3.018737
P(T<=t) one-tail 0.007254
t Critical one-tail 1.833113
P(T<=t) two-tail 0.014509
t Critical two-tail 2.262157
Table 7.13. The one-tailed t-test result between N-based and K-based in terms

of fresh leaf mass.

t -Test: Paired Two Samples for Means


P-based K-based
Mean 5.0446 4.401
Variance 2.088016 0.603715
Observation 10 10
Pearson Correlation -0.66376
Hypothesized Mean Difference 0
Df 9
t Stat 0.892571
P(T<=t) one-tail 0.197663
t Critical one-tail 1.833113
P(T<=t) two-tail 0.395325
t Critical two-tail 2.262157
Table 7.14. The one-tailed t -test result between P-based and K-based in terms

of fresh leaf mass.


84

t -Test: Paired Two Samples for Means


N-based No fertilizer
Mean 3.04235 1.9002
Variance 0.685948 0.516811
Observation 10 10
Pearson Correlation -0.2757
Hypothesized Mean Difference 0
Df 9
t Stat 2.918817
P(T<=t) one-tail 0.008532
t Critical one-tail 1.833113
P(T<=t) two-tail 0.017065
t Critical two-tail 2.262157
Table 7.15. The one-tailed t -test result between N-based and no fertilizer in

terms of dry leaf mass.

t-Test: Paired Two Samples for Means


P-based No fertilizer
Mean 2.9435 1.9002
Variance 0.412851 0.516811
Observation 10 10
Pearson Correlation -0.47949
Hypothesized Mean Difference 0
Df 9
t Stat 2.816002
P(T<=t) one-tail 0.010089
t Critical one-tail 1.833113
P(T<=t) two-tail 0.020178
t Critical two-tail 2.262157
Table 7.16. The one-tailed t -test result between P-based and no fertilizer in

terms of dry leaf mass.


85

t -Test: Paired Two Samples for Means


K-based No fertilizer
Mean 2.6459 1.9002
Variance 0.603715 0.516811
Observation 10 10
Pearson Correlation 0.207592
Hypothesized Mean Difference 0
Df 9
t Stat 2.501541
P(T<=t) one-tail 0.016888
t Critical one-tail 1.833113
P(T<=t) two-tail 0.033776
t Critical two-tail 2.262157
Table 7.17. The one-tailed t -test result between K-based and no fertilizer in

terms of dry leaf mass.

t -Test: Paired Two Samples for Means


N-based P-based
Mean 3.04235 2.9435
Variance 0.685948 0.412851
Observation 10 10
Pearson Correlation 0.129477
Hypothesized Mean Difference 0
Df 9
t Stat 0.318711
P(T<=t) one-tail 0.378609
t Critical one-tail 1.833113
P(T<=t) two-tail 0.757218
t Critical two-tail 2.262157
Table 7.18. The one-tailed t -test result between N-based and P-based in terms

of dry leaf mass.


86

t -Test: Paired Two Samples for Means


N-based K-based
Mean 3.0423 2.6459
Variance 0.685948 0.603715
Observation 10 10
Pearson Correlation -0.02678
Hypothesized Mean Difference 0
Df 9
t Stat 1.089353
P(T<=t) one-tail 0.152153
t Critical one-tail 1.833113
P(T<=t) two-tail 0.304306
t Critical two-tail 2.262157
Table 7.19. The one-tailed t -test result between N-based and K-based in terms

of dry leaf mass.

t -Test: Paired Two Samples for Means


P-based K-based
Mean 2.9435 2.6459
Variance 0.412851 0.603715
Observation 10 10
Pearson Correlation -0.02976
Hypothesized Mean Difference 0
Df 9
t Stat 0.824213
P(T<=t) one-tail 0.215559
t Critical one-tail 1.833113
P(T<=t) two-tail 0.431119
t Critical two-tail 2.262157
Table 7.20. The one-tailed t -test result between N-based and K-based in terms

of dry leaf mass.


87

t -Test: Paired Two Samples for Means


N-based No fertilizer
Mean 195.6 165.1
Variance 1606.489 1569.656
Observation 10 10
Pearson Correlation -0.12389
Hypothesized Mean Difference 0
df 9
t Stat 1.61432
P(T<=t) one-tail 0.070458
t Critical one-tail 1.833113
P(T<=t) two-tail 0.140916
t Critical two-tail 2.262157
Table 7.21. The one-tailed t -test result between N-based and no fertilizer in

terms of final height.

t -Test: Paired Two Samples for Means


P-based No fertilizer
Mean 228.5 165.1
Variance 1999.833 1569.656
Observation 10 10
Pearson Correlation 0.23119
Hypothesized Mean Difference 0
df 9
t Stat 3.02636
P(T<=t) one-tail 0.007165
t Critical one-tail 1.833113
P(T<=t) two-tail 0.014331
t Critical two-tail 2.262157

Table 4.22. The one-tailed t -test result between P-based and no fertilizer in

terms of final height.


88

t -Test: Paired Two Samples for Means


K-based No fertilizer
Mean 217.1 165.1
Variance 666.9889 1569.656
Observation 10 10
Pearson Correlation 0.226077
Hypothesized Mean Difference 0
df 9
t Stat 3.90415
P(T<=t) one-tail 0.001798
t Critical one-tail 1.833113
P(T<=t) two-tail 0.003597
t Critical two-tail 2.262157
Table 4.23. The one-tailed t -test result between K-based and no fertilizer in

terms of final height.

t -Test: Paired Two Samples for Means


P-based N-based
Mean 228.5 195.6
Variance 1999.833 1606.489
Observation 10 10
Pearson Correlation -0.77004
Hypothesized Mean Difference 0
df 9
t Stat 1.303876
P(T<=t) one-tail 0.112318
t Critical one-tail 1.833113
P(T<=t) two-tail 0.224637
t Critical two-tail 2.262157
Table 4.24. The one-tailed t -test result between P-based and N-based in terms

of final height.
89

t -Test: Paired Two Samples for Means


K-based N-based
Mean 217.1 195.6
Variance 666.9889 1606.489
Observation 10 10
Pearson Correlation -0.02121
Hypothesized Mean Difference 0
df 9
t Stat 1.412339
P(T<=t) one-tail 0.095741
t Critical one-tail 1.833113
P(T<=t) two-tail 0.191481
t Critical two-tail 2.262157
Table 4.25. The one-tailed t -test result between K-based and N-based in terms

of final height.

t -Test: Paired Two Samples for Means


P-based K-based
Mean 228.5 217.1
Variance 1999.833 666.9889
Observation 10 10
Pearson Correlation -0.24537
Hypothesized Mean Difference 0
df 9
t Stat 0.63396
P(T<=t) one-tail 0.270941
t Critical one-tail 1.833113
P(T<=t) two-tail 0.541882
t Critical two-tail 2.262157
Table 4.26. The one-tailed t -test result between P-based and K-based in terms

of final height.
90

Appendix C
(Documentation)
91

Germination of water spinach Water spinach seeds after


seeds germination process

Water spinach shoots on egg Transplanting water spinach


tray shoots to containers

Water spinach subjects on the Application of organic


1st week of planting fertilizer to water spinach
92

Water spinach subjects on the Water spinach subjects on the


2nd week of planting 3rd week of plant

Weighing the dry leaf mass of The N-based organic liquid


water spinaches fertilizer

The N-based organic liquid The K-based organic liquid


fertilizer fertilizer
93

CURRICULUM
VITAE
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Name: Crystal Jean B. Agustin

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