BIONICS PROFILE

BIONICSENVIROTECH

Innovative Technologies for Biological Treatment

Submitted

by

Dr.M.PARAMESWARI Ph.D (AGRI. ENVIRO.), DIRECTOR –TECHNICAL

BIONICS ENVIRO TECH

PROPOSAL FOR THE IMPLEMENTATION OF MICROBIAL NANOZYME TO COMMON EFFLUENT

TREATMENT PLANT

Mr. K. Sasikumar MCA., Managing Director

Dr. M.Parameswari , PhD Agri. Enviro, Technical Director

B.Rajesh – General Manager Technical

 Our Contacts
Company Name: BIONICS ENVIRO TECH

Company Address:
2/185, RAM NAGAR, VAAIKALMEDU,

PERUNDURAI MAIN ROAD ERODE-638052, TamilNadu

Tel.: 9095000090

E-Mail: drparams2014@gmail.com, bionicsenvirotech@gmail.com,

info@bionicsenviro.com
Website: www.bioncisenviro.com
Contact Persons: Dr.M.Parameswari K. Shasikumar
Mobiles: 9095000090 8220227745
E-mails: drparams2014@gmail.com Kshaikumar@hotmail.com

BIONICS OFFCIES

HEAD OFFICE CORPORATE OFFICE REGIONAL OFFICE

2/185, RAM NAGAR, NO-1, GST ROAD, PRATAP COLONY, NEAR

VAAIKALMEDU, THAILAVARAM, POTHERI HOLY CHILD SCHOOL
PERUNDURAI MAIN ROAD CHENNAI-603203 RUHATTA, JAUNPUR
ERODE-638052 SADAR
UTTAR PRADESH-222
002
JOURNALS INTERNATIONAL RESEARCH PAPERS PUBLISHED.

1. Parameswari. M. 2013.Evaluating the impact of textile and dye effluent

irrigation and amendments on major nutrient nitrogen status of soil under
maize crop. Elixir International J. 65:20174-76. Current Index Copernicus,
Poland Value (ICV) is 5.09. ISSN: 2229-712 X.

2. Parameswari.M. 2013. Impact of textile and dye industry liquid and solid
waste and amendments on potassium status of soil under sunflower crop.
Elixir International J. 65:20180-82. Current Index Copernicus, Poland Value
(ICV) is 5.09. ISSN: 2229-712 X.

3. Parameswari. M. 2013. Evaluation of textile and dye industry liquid and

solid waste and amendments on exchangeable magnesium content of soil
under sunflower crop. Elixir International J. 65:20177-79. Current Index
Copernicus, Poland Value (ICV) is 5.09. ISSN: 2229-712 X.

4. Parameswari. M and C. Udayasoorian. 2013. Influence of textile and dye

effluent irrigation and amendments on Micronutrients Iron and Copper
status in soil under Maize crop. INT. J. Cur. Tr. Res. (2013) 2(1):163-167.ISSN
2278-8042

5. Parameswari. M and C. Udayasoorian 2013. Effect of textile and dye

effluent irrigation and amendments on exchangeable calcium and
magnesium content of soil under maize crop. INT. J. Cur. Tr. Res. (2013)
2(1):132-135. ISSN 2278-8042
6. Parameswari. M and C. Udayasoorian 2013 Impact of textile and dye
industry liquid and solid waste and amendments on phosphorus status of
soil under sunflower crop. International Res. J. of Biol. Sci. Vol. 2(6) ISSN
2278-3202.

7. Parameswari. M 2013. Vermicomposting of textile and dye sludge with

carbonaceous materials and its carbon and nitrogen status, Elixir
International J. 66: 21104-05.Current Index Copernicus, Poland Value (ICV)
is 5.09. 64A (2013) 19477-19480. ISSN: 2229-712 X.-

8. Parameswari. M 2013. Vermistabilisation of textile and dye sludge with

organic wastes and its phophorus and potassium value. Elixir International
J. 64A (2013) 19473-19476Current Index Copernicus, Poland Value (ICV) is
5.09. ISSN: 2241-712 X

9. Parameswari.M 2013. Micronutrients zinc, manganese, iron and copper

status of sunflower cultivated soil under textile and dye effluent and sludge
application with amendments. International J. Of Appli. and Natl. Sci. (
IJANS ); ISSN: 2319-.

10. Parameswari. M 2013. Textile And Dye Industry Effluent, Sludge And
Amendments On Dehydrogenase And Phosphatase Activity Of Soil Under
Sunflower Crop. International J. Of Appli. and Natl. Sci. ( IJANS ); ISSN: 2319-
4022.

11. Parameswari. M 2013. Textile and dye industry effluent, sludge and
amendments on heavy metals chromium, nickel, cadmium and lead status
of maize cultivated soil International J. Of Appli. and Natl. Sci. ( IJANS );
ISSN: 2319-4022.

12. Parameswari. M, Sumathi.M, 2012, Nitrogen transformation during

composting of poultry waste, International conference on Biologically
Active Molecules (ICBAM 2012). 98-113 Organized by Department of
Chemistry, Gandhigram Rural Institute- Deemed University, Dindigul,
TamilNadu, India Title of the Research Papers Accepted

13. Parameswari.M and C. Udayasoorian (2012) Evaluating The Nitrogen,

Phosphorus and Potassium Phytoremediation Efficiency Of Sunflower In
Textile And Dye Effluent Polluted Soil Habitat. WATER R&D. Vol.2, No.1,
2012

14. Parameswari.M and C. Udayasoorian (2012). Impact of various

concentration of textile and dye effluent and sludge on soil fertility, growth
and yield of maize crop. RASAYAN J. of Chemistry.vol-5, No.3,July-
September(2012) ISSN No.0974-1496.

15. Parameswari.M and C. Udayasoorian. 2013. Impact of dye effluent

irrigation with amendments on Micronutrients zinc and Manganese status
in soil under sunflower crop. Elixir International J. Current Index
Copernicus, Poland Value (ICV) is 5.09. ISSN: 2229-712.
NATIONAL JOURNAL RESEARCH PAPERS PUBLISHED

1. Parameswari. M and C. Udayasoorian (2013) Impact of textile and dye

effluent irrigation on the cereals like Paddy and Maize crops and its growth
parameters Journal of Environmental Science and Sustainability (JESS) Vol.1
(2): 69 – 73, 2013. ISSN: 2321-5577.

2. Parameswari. M and C. Udayasoorian (2012) Impact of textile and dye

effluent irrigation on germination and its growth in cereals. Res. Environ.
Life Sci., 6(2) 69-72 (2013). ISSN: 0974-4908.

3. Parameswari. M and C. Udayasoorian (2013) Influence of different

concentration of textile and dye effluent and sludge on soil fertility, growth
and yield of maize crop. Journal of Environmental Science and Sustainability
(JESS) Vol.1 (2): 61 – 65, 2013. ISSN: 2321-5577

4. Parameswari. M and C. Udayasoorian (2013). Evaluation of textile and

dye industry liquid and solid waste and amendments on Exchangeable
magnesium content of soil under sunflower crop,. Journal of Environmental
Science and Sustainability (JESS) Vol.1 (3): 89 – 92, 2013. ISSN: 2321-5577.

5. Parameswari. M and C.Udayasoorian (2013). Vermicomposting of textile

and dye sludge with carbonaceous materials and its carbon and nitrogen
status. Journal of Environmental Science and Sustainability (JESS) Vol.1 (3),
93-96 2013. ISSN: 2321-5577
6. Parameswari. M 2013. Phytoremediation efficiency of sunflower crop
under polluted soil habitat irrigation with textile and dye industry effluent
and sludge with amendments. Res. Environ. Life Sci. 6(4)125-128 (2013).
ISSN: 0974-4908 National Research Papers

7. Parameswari. M 2013. Influence of dye effluent irrigation with

amendments on micronutrients like zinc and manganese status in soil
under sunflower crop. Journal of Environmental Science and Sustainability
(JESS) Vol.2 (3): 59-61, 2013.

8. Parameswari. M. and C.Udayasoorian (2013). The impact of textile and

dye effluent irrigation and amendments on phosphorus status of soil under
maize crop, Environmental Science: Processes & Impacts.

9. Parameswari. M and C. Udayasoorian 2013. Textile And Dye Industry

Effluent, Sludge And Amendments On Dehydrogenase And Phosphatase
Activity Of Soil Under maize crop. Journal of Environmental Research And
Development .
SEMINARS/CONFERENCES/TRAININGS PAPER PRESENTED. NAME OF THE
SEMINAR / CONFERENCE/TRAINING-RESEARCH PAPERS PRESENTED.

1. Parameswari.M, S.Avudainayagam, 2004, Ammonical nitrogen

concentration during poulty waste composing,National seminar on New
frontiers of soil science Research towards sustainable Agriculture
(NEFROSSA- 2005) Dept. of soil science , Annamalai university & UGC

2. Parameswari.M, S.Avudainayagam, 2004, Organic Farming, National

seminar on conservation of agro – biodiversity in India – The Role of
stakeholders. Dr.G.R. Damodaran Colllege of Science, Cbe, Ministry of
Environment and Forest, Govt. of India.

3. Parameswari.M, Avudainayagam.S, 2005, Reduction of ammonia

volatization during composting of poultry waste, National conference on
frontiers in Environmental sciences & Engineering in India. Dept. of Env. Sci.
Bharathiyar Univ. ,Cbe & National Environmental Engineering Research
Institute, Nagpur.

4. Parameswari.M, Avudainayagam.S, 2005, Changes in calcium and

magnesium content during composting of poultry waste. National
symposium on Microbial Technology for productive Agriculture. Dept. of
Agricultural Microbiology, TNAU, Cbe.

5. Parameswari.M, Avudainayagam.S, Nithyadevi .A, 2005, Aerobic

composting of poultry waste with microbes for reduction of ammonia
volatiliazation , 4th National level biological congress on biotechnology – A
Boon to Humanity – 2005 , C-23 Muthayammal College of
Engineering,Namakkal. CSIR New Delhi, TNSCST Chennai.

6. Parameswari.M, Avudainayagam.S, Nithyadevi.A, 2005, Micronutrient

status and microbial changes during poultry waste composting, 4th
National level biological congress on biotechnology – A Boon to Humanity –
2005 , C-24 Muthayammal College of Engineering,Namakkal CSIR New
Delhi, TNSCST Chennai

7.Nithyadevi.A, Parameswari.M, Avudainayagam.S, 2005, Terminator,

Verminator and traiter technologies, 4th National level biological congress
on biotechnology – A Boon to Humanity – 2005 , C-24 Muthayammal
College of Engineering,Namakkal CSIR New Delhi, TNSCST Chennai

8.Nithyadevi.A, Parameswari.M, Avudainayagam.S, 2005, Evaluation of

antagonistic potential of trichoderma harzianum against banana
anthracnose, 4th National level biological congress on biotechnology – A
Boon to Humanity – 2005, E-8 Muthayammal College of Engineering,
Namakkal CSIR New Delhi, TNSCST Chennai .

9. Parameswari.M, Avudainayagam.S 2004, Aerobic composting of different

systems of poultry wastes, Southern Regional conference on microbial
inoculants, conducted by university of Agricultural Sciences, Bijapur-
Karnataka Dept. of Agricultural Microbiology, University of agricultural
Sciences Dharwad.
International Seminar/Conference- Research papers published Name of the
Seminar/Conference/ Training Organized

• Parameswari.M, Sumathi.M, 2012, Nitrogen transformation during

composting of poultry waste, International conference on Biologically
Active Molecules (ICBAM 2012). 98 Gandhigram Rurl university, Dindigul,
and University Grants Commission, New Delhi, Gandhigram Rural Institute .

• Parameswari.M, Sumathi.M and Esakkiammal.M 2012, international

workshop on climate change in agriculture; Adaptation and mitigation
strategies Impact of textile and dye effluent irrigation on the cereals like
Paddy and Maize crops and its growth parameters. Organized by Faculty of
Agriculture and Animal Husbandry Gandhigram Rurl university, Dindigul,
and University Grants Commission, New Delhi, Gandhigram Rural Institute
ABOUT BIONICS ENVIRO TECH

BIONICS ENVIRO TECH is an industry world leader in the field of biological

waste water treatment by manufacturing Nanozyme Bioculture and
implementing it in all industries. With presence in all states, BIONICS
ENVIRO TECH with strong Research & Development and a passion for
innovation is offering purely Nanozyme technologies with wide range of
solutions mainly focused on COD, BOD Reduction, Zero Sludge Process,
Zero Pollutant load in directed at addressing wastewater challenges in all
industries Effluent Treatment Plants Treatment Plants (CETP) and
Anaerobic Digester (AD) of any scale, any sector across the world with track
record.

Industrialization improves the economic value of a nation but

simultaneously it leads to the degradation of environment. In the present
proposal for the removal of pollutants from the Wastewater treatment
plants and CETPs by using innovative NANOZYME (Effective Microbes (EM)-
bacterial consortium manufactured in BIONICS ENVIRO TECH having
potential to degrade lingo-cellulosic compounds and other inorganic and
inorganic matter present in the industries.
WHAT IS NANOZYME ?

1. NANO-ZYME” which are Effective Microbes (EM) contain multiple strains of

aerobic and facultative anaerobic microbes, which are selected for their
compatible and specific enzyme, symbiotic metabolic pathways based on
different industrial effluent characteristics.

2. NANOZYME products are manufactured from a unique solid state

fermentation (SSF) process of selected cultures of beneficial bacteria.

3. Available Form: NANOZYME is in Paste Form.

4. Mixture of beneficial microbes which are 50-62 different strains of bacteria

based on Effluent characteristics.

AVAILABLE FORM ORGANIC SEMI-SOLID FORM

Number Of Bacterial Strains 60 Different Bacterial Strains

Stage Of Bacteria Living Bacteria

Number Of Bacterial Colonies 63 X 10-9 CFU/Ml

ROLE OF MICROBIAL NANOZYME IN WASTE WATER TREATMENT PLANTS:

PARAMETERS PERCENTAGE OF REDUCTION

Sludge Degradation Zero sludge

Chemical Oxygen Demand (COD) 95-98%

Biological Oxygen Demand (BOD) 98-100%

Color 90-100%

Odor 100%

Removal Of Suspended &

100%
Floatable Organic Debris

Reduces Total Suspended Solids

92-95%
(TSS)

Reduces Total Dissolved Solids

Depends on Characteristics
(TDS)

Increases Dissolved Oxygen (DO) Min. 3 - max - 6

NANOZYME USAGE IN INDUSTRY:

TEXTILE & DYE PROCESSING INDUSTRIES

PAPER AND PULP INDUSTRIES

TANNERIES & LEATHER

PHARMACEUTICAL INDUSTRIES

SUGAR & DISTILLERIES INDUSTRIES

MILK & DAIRY INDUSTRIES

BREWERIES, WINE & ALCOHOL PLANTS

NANOZYME APPLICATION :

Aeration Tank

Anaerobic digestion

Collection and Equalization Tank

Common Effluent Treatment Plants

(CETP)

Effluent Treatment Plants (ETP)

Sewage Treatment Plants (STP)

RANGE OF NANOZYME PRODUCTS

EFFLUENT TREATMENT PLANT

NANOZYME - BET - ETP - 7001 < 500 COD

NANOZYME - BET - ETP - 7002 500 - 2000 COD

NANOZYME - BET - ETP - 7003 > 2000 COD

Available in 50 Kgs and More. Dosage : 50 Kgs. / 100 KLD

SEWAGE TREATMENT PLANT

NANOZYME - BET - STP – 9011 < 300 COD

NANOZYME - BET - STP – 9012 300 - 1000COD

NANOZYME - BET - STP – 9013 > 1000 COD

Available in 50 Kgs and More. Dosage : 50 Kgs. / 100 KLD

 ANAEROBIC

NANOZYME BET- ANA-9001 NANOZYME - BET - BIOG - 7501

NANOZYME BET- ANA-9002 NANOZYME - BET - BIOG – 7502

NANOZYME BET- ANA-9003 NANOZYME - BET - BIOG – 7503

Available in 50 Kgs and More. Dosage : 50 Kgs. / 100 KLD

COMPOSTING

NANOZYME BET- COMP-7101

NANOZYME BET- COMP-7102

NANOZYME BET- COMP-7103

Available in 50 Kgs and More.

Dosage : 50 Kgs. / 100 KLD

How do BACTERIA breakdown any MOLECULE

Nanozyme contains 60 different bacterial strains, each

selected continually adapt and grow at degrading certain
waste materials. Biological treatment is the only method that
can degrade the waste.

Bacteria have the capability of producing many different

types of enzymes that degrade a wide variety of organic
materials such as fats, oils, cellulose, xylan, proteins starches
and all chemicals

Bacteria can produce the complete "team" of enzymes that

are necessary to degrade and consume the organic materials
present in their environment at any given time.

They can protect themselves from changes in environmental

conditions by forming colonies, biofilms, or spores.

Bacteria can adapt to a range of conditions and food supplies.

They can change the type of enzymes that they produce if the
food source changes.
How quickly do high enzyme producing bacteria and in what quantities?

• Production of enzymes begins as soon as the bacteria begin to grow. The

cells must obtain nutrients from their surroundings, so they secrete
enzymes to degrade the available food.

• The quantities of enzymes produced vary depending on the bacterial

species and the culture conditions (e.g., nutrients, temperature, and pH)
and growth rate.

• Hydrolytic enzymes such as proteases, amylases, and cellulses, etc. are

produced in the range of milligrams per liter to grams per liter.

ACTIVATED SLUDGE SYSTEMS

NANOZYME PREPARATION METHOD

Dosage: 50 kgs/ 100 KLD (For one month’s) (It will vary depends on COD, BOD,
TDS & Nutrient status of the Effluent).

PREPARATION METHOD:

NANOZYME 50 KG + NUTRIENTS 50 KG MIXED WITH 1000 LITERS OF WATER

↓ Keep it as such Incuba on 3 Days

NANOZYME BACTERIAL CULTURE READY FOR USAGE

Application Dosage details of incubated NANOZYME Bacterial cultures

From 1000 litres of prepared broth Volume

Dosage Day of Dosage of Nanzoyme Application to aeration tank
each dosage

1st Dosage 1st Day 50 Litres

2nd Dosage 2rd Day 50 Litres

3rd Dosage 3rd Day 50 Litres

4th Dosage 4th Day 50 Litres

5th Dosage 5th Day 50 Litres

6th Dosage Alternate Day 50 Litres

Subsequent Vary Depends on

dosage parameters 50 Litres
IMPORTANT ABOUT NANOZYME
COMPLEMENTARY SERVICES

• NANOZYME can solve the most complex wastewater treatment.

• Our experienced doctorates , professionally-trained staff and well equipped

laboratory facilities are available to provide a wide range of services,
including Treatment Plant .

• Assessment, Microscopic Biomass Examinations, Chemical Analysis, Bio-

treatability Testing, Wastewater Toxicity Testing, Bioremediation Studies
and Process Scale-up, Process Design and Development.

• Our production facilities and services are available at an hour of reach to

support you.

ARE REGULAR MAINTAINENCE APPLICATIONS REQUIRED?

• Usually yes. The augmented microbes may die out or be present in too low
a number after a period of low influent concentration to be effective when
the waste strength increases rapidly.

• Other conditions (pH, temperature, waste flow rate, alkalinity) will also vary
causing fluctuations in the population dynamics (relative numbers of
different microbes) of the system.
 • For these reasons, regular maintenance application is recommended in
almost every case.

• Some exceptions are required for start-up assistance and one-time batch
operations. For longer term batch wise treatment re-inoculation is
required as site conditions change.

NANOZYME for composting of solid waste:

• Range of products available for all type of solid waste

• Municipal solid waste

• Industrial sludge composting

• Agricultural waste composting

AREAS OF APPLICATIONS:

Municipalities; Food Industries, Pharmaceutical, Oil Fields, Petrochemicals, Power

Plants, Rural Areas,

RESEARCH & DEVELOPMENT IN LABORATORIES

1. In BIONICS ENVIRO TECH, we step new paths and break new foundation
with technological excellence.

2. Our products are formulated with intensive Research and Development

which suit specific needs of customers.
 3. Products are manufactured with stringent laboratory standards
incorporating modern equipment’s using international formulas - to
achieve ISO- 9001 standards and NABL accreditation for laboratories.

GAPS IN EXISTING CONVENTIONAL METHOD OF TREATMENT SYSTEM

The physical and chemical methods are not feasible techniques since they are
very expensive, experience operational problems and formation of hazardous by-
products or intensive energy requirement.

 Although the physical and chemical methods are on the track of treatment,
they are not on par with biological treatment because of cost
ineffectiveness and residual effects.

 The biological treatment is known to be effective in reducing the organic

load and toxic effects of paper mill effluents.

 None of the available conventional methods are permanent eco-friendly

disposal solution.

 Biological methods have been acknowledged for the degradation of

effluents.

 Biological methods involve microorganisms capable of degrading all waste

in natural environments.

 The biological colour removal process uses several classes of bacteria to

degrade the polymeric lignin derived chromophoric material.
  Several methods have been attempted by various researchers throughout
the world for the removal of colour from effluents.

 Being Energy Intensive and more expensive these methods were not
implemented at industrial sites.

WHY WE GO FOR INNOVATIVE MICROBIAL TECHNOLOGY BY USING NANOZYME ?

Compared with physicochemical ways, biological methods for wastewater

treatment are considered to be of cost benefit, eco-friendly and suitable for
reduction of the BOD and COD from the effluents. However, the conventional
biological processes have not effectively performed for removal of colour and
recalcitrant compounds from wastewater. Bioremediation is a pollution control
technology that uses biological systems to catalyze the degradation or
transformation of various toxic chemicals to less- harmful forms. So,
bioremediation is employed for the treatment of various industrial effluents .

a) This conventional treatment system is used in treatment of several

industrial wastewater types in order to remove COD, BOD, SS, and AOX. but
industries achieve only 70% BOD and 60% COD removal efficiency at
industrial effluent, even in full-scale activated sludge systems. this is
because of lack of selective degrading bacteria.

b) Industry uses conventional activated sludge treatment process in which

they are using nonspecific microorganisms and they kept food/microbe
(F/M) ratio low in the aeration tank.
c) A plant operating at low F/M ratio spectrum is characterized by a high
concentration of sludge.

d) Chemical oxidation/precipitation methods are tedious, provide an

additional environmental load. Biological methods are of-ten preferred
since it has many advantages like rapid biodegradation rates, low sludge
yield and excellent process stability.

e) E. Biological methods are of particular interest because they can also

reduce chemical and bio-logical demands (COD, BOD), which are also
significant problem in wastewater and so reduce holding times in aeration
and sedimentation ponds prior to wastewater discharge into the
environment.

f) So the intention of this project proposal is to implement the predominant

Effective bacteria (NANOZYME) to the MBBR and Aeration tank of the
paper and pulp effluent to decrease the degradation efficiency of the
bacteria.

a. The researchers are more focused on environmental friendly

technologies for the treatment ofwastewater. Therefore they use
biological approach for the removal of contaminants from the
effluent. The biological treatment processes able to reduce
colour, COD, BOD, and toxic low molecular weight chlorinated lignin
derivatives.
 b. A number of research studies have discovered that a group of
extracellular isoenzymescalled ligninases which are lignin peroxidase
(LiP), manganese-dependent peroxidase (MnP) and lactase produced
by some microorganisms are capable of degrading lignin present in
the effluent. some researchers evaluated the ability of these
bacteria's fluent treatment. Some investigators have made an
attempt to treat wastewater by the thermophilic temperature at
which they are discharged to reduce energy cost for treatment.

c. Some of researchers reported autochthonous bacteria have the

ability to use lignin, tannic acid, xylan, and cellulose as carbon source
and reduce the COD value up to 86.5% (back water) and 65%
(80 : 20, back water and black liquor mixture) of effluent generated
from small (agro-based).

NEED TO IMPLEMENT THE MICROBIAL NANOZYME TECHNOLOGY ?

In recent past, the colour of effluent discharge into waterways has become
important problem. All Industrial effluent has recognized as environmental
hazards and categorized one of the twelve most polluting industry in our country.
The dark brown colour of the effluent is mainly due to their high contents of
oxidized and partially degraded lignin. Reducing this colour before the effluent is
mainly due to their high contents of oxidized and partially degraded lignin.
Reducing this colour before the effluents are discharged into natural water is an
important goal. Therefore, more advanced alternative biological wastewater
treatment strategies will be required for the treatment of effluent holistically and
meet the new and more stringent discharge limits set for paper industry effluent.
Such new biological treatment technologies must be developed to degrade lingo-
cellulosic compounds and halogenated chemicals in effluents that pose the
greatest threat to human health.

Our specific novel NANOZYME effective microbes which can survive under such
extreme environment and can effectively degrade organic matter present in the
pulp and paper industry effluent. This formulation has been derived after a 15
years long trial process. It has been specifically formulated to break down through
aerobic and facultative anaerobic action, all the biodegradable substances in the
wastewater. It is also formulated with H2S degrading bacteria and able to work in
a pH range of 2.0 to 13.0.

ROLE OF MICROBES IN THE DEGRADATION OF EFFLUENT

An enzyme is a globular protein with an active site which bind to substrate

molecules and helps to catalyse a reaction by holding molecules in the correct
spatial conformation for the reaction to take place. The activities of the enzymes
are determined by their 3-dimensional structure. Most enzymes are much larger
than the substrates they act on and only a small portion of the enzyme around 3-
4 aminoacids is directly involved in catalysis.

Our products are based on the latest advanced technology in biotechnology

designed to meet the requirements of specific waste water treatment problems.
Biological treatment method by microbes is the only method that can
eliminate/degrade the waste problem. Specially selected and acclimated strains
of microorganisms supplied in NANOZYME microbial enzyme products produce
millions of times the levels of organic digesting catalysts produced by various type
of microorganisms found naturally in different waste. Bacteria are living
organisms that continually adapt and grow in the system. They consume the
waste, chemicals, medicals.

Effective Microorganism (EM) is the consortia of valuable microorganisms which

secretes organic acids and enzymes for utilization and degradation of
anthropogenic compounds. Combination of various strains will successively
promotes the growth of bacterial population, break down and digest the waste in
both aerobic and anaerobic conditions to a far greater degree than single
microbial strain. Bioremediation process involves detoxification and
mineralization, where the waste is converted into inorganic compounds such as
carbon dioxide, water and methane. When compounds are persistent in the
environment, their biodegradation often proceeds through multiple steps utilizing
different enzyme systems or different microbial populations.

BACTERIAL BIOREMIDIATION

Number of bacterial species has been assessed for their decolorization abilities
and a few of them have also been used commercially. The dominant aboriginal
microbes were found competent of reducing BOD up to 87.2%, COD up to 94.7%
and lignin content up to 97%. It has been observed that although, numerous
bacteria can decompose monomeric lignin substructure models, only a few strains
are able to attack lignin derivatives obtained from different pulping processes.
some of microbial enzyme was successfully used in a large-scale bio-bleaching
that bleaches pulp optimally at pH 9 and 65°C temperature
 1. NANOZYME effectively treats the suspended and floatable organic
debris.

2. Reduces more than 95% COD (Chemical Oxygen Demand),

3. Reduces more than 96 %BOD (Biological Oxygen Demand),

4. Reduces TSS (Total Suspended Solids) and 50% of TDS (Total dissolved
solids)

5. Cultures grow in either the aerobic and anaerobic conditions

6. LOW COST TREATMENT and Increases efficiency of the Effluent

treatment plant and saves energy costs.

7. Nanozyme is purely organic so, Non-corrosive, non-pathogenic and low

quantities of use, making it safe and easy to handle and store.

8. Results meet criteria for new Pollution Control norms.

9. No design or process modifications required

The importance of ligninolytic bacteria raised, because lignin-degrading bacteria

have wider tolerance of temperature, pH and oxygen limitation.
REACTION BY BACTERIA WITH POLLUTANTS

1. Bacteria break down organic matter into simple compounds like carbon
dioxide and water, and they cycle important nutrients such as nitrogen,
sulfur, and phosphorus.

2. The enzymes enable the microbes to use the organic contaminants as

sources of energy and/or as reproductive building blocks.

3. Bacteria can migrate to areas that are rich in specific nutrients that they
require for growth. Bacteria can also attach themselves to surfaces and
form communities known as biofilms.

4. The bacteria function as millions of tiny enzyme factories to produce the

correct balance of degradative power.

5. Bacteria can produce the complete "team" of enzymes that are necessary
to degrade and consume the organic materials present in their
environment at any given time

6. Bacteria can adapt to a range of conditions and food supplies. They can
change the type of enzymes that they produce if the food source changes.
They can protect themselves from changes in environmental conditions by
forming colonies, bio films, or spores
 7. Production of enzymes begins as soon as the bacteria begin to grow. The
cells must obtain nutrients from their surroundings, so they secrete
enzymes to degrade the available food.

8. The quantities of enzymes produced vary depending on the bacterial

species and the culture conditions (e.g., nutrients, temperature, and pH)
and growth rate. Hydrolytic enzymes such as proteases, amylases, and
celluloses, etc. are produced in the range of milligrams per liter to grams
per liter. Bacteria can grow very quickly, doubling their populations in as
little as 20-40 minutes. In some applications, it is common to "boost"
bacterial colonization.

CELLULOSE DEGRADATION BY BACTERIA

Cellulose is a structural polysaccharide that contains glucose. In order to access

this glucose for catabolism, the cellulose must be cleared by extracellular
enzymes. These pieces are then transported into the cell for energy generation
(catabolism) or production of biomass (anabolism). Some bacteria are important
participants in the extracellular cleavage of cellulose.
LIGNIN DEGRADATION BY BACTERIA

Lignin is the second most abundant renewable biopolymer in nature after

cellulose, yet it is degraded by only a small number of microorganisms.
Ligninolytic enzymes are involved in the degradation of the complex and
recalcitrant polymer lignin. This group of enzymes are highly versatile in nature
and they find application in a wide variety of industries. These aromatics can be
released from the lignin sturucture by microbial enzymes such as peroxidases and
oxidases. The enzymes utilize H2O2 and OH radicals to break the bonds in the
lignin. Peroxidases are classified as oxido reductases which are used for catalyzing
various oxidative reactions. They are isolated from various sources like plants,
animals and microbes. Peroxidases have wide applications in many areas like
industrial, medical and food processing. Enzymes carry out transformation of
molecules and also mediate transformation of energy. The term peroxidase in the
widest sense includes a group of specific enzymes such as NAD peroxidase, fatty
acid peroxidases, glutathione peroxidase as well as a group of very nonspecific
enzymes from different sources simply known peroxidases

In the present proposal for the removal of pollutants from the sewage water by
using NANOZYME (Effective Microbes (EM)- bacterial consortium manufactured in
BIONICS ENVIRO TECH having potential to degrade all organic and inorganic
compounds present in the Effluent water.
HOW BACTERIA AND ENZYMES WORK TO DIGEST ORGANIC WASTES?

This process is responsible for the digestion of organic waste.

BACTERIAL / ENZYME DIGESTION

Bacterial digestion is the process of bacteria, consuming organic matter. Enzymes

act to break the organic matter into water soluble nutrients, which the bacteria
digest. Using complex chemical reactions, the organic waste is metabolized down
to water and carbon dioxide (the final metabolic waste products), providing the
bacteria with energy for growth and reproduction. It may be simply shown by the
following equation:

AEROBIC DIGESTION

Organic waste + water -----Enzyme----> Water soluble nutrients + oxygen ----

Bacteria---> water + carbon dioxide

ANAEROBIC DIGESTION

Organic waste + Water ------Enzyme----> Water Soluble Nutrients -----Bacteria---

> Water + Carbon Dioxide

Organic waste is consumed by the bacteria, used as nutrients by the bacteria, and
is no longer present to produce odors, sludge, pollution, or unsightly mess.
WHERE TO APPLY NANOZYME IN TO YOUR TREATMENT SYSTEM

1. Collection/Equalization tank

2. Aeration Tank

1. Collection/Equalization Tank

Bacteria can produce the complete "team" of enzymes that are necessary to
degrade and consume the organic materials present in equalization tank. Bacteria
can adapt to a range of conditions and food supplies. They can change the type of
enzymes that they produce if the food source changes. They can protect
themselves from changes in environmental conditions by forming colonies,
biofilms, or spores. Production of enzymes begins as soon as the bacteria begin to
grow. The cells must obtain nutrients from their surroundings, so they secrete
enzymes to degrade the available food. The quantities of enzymes produced vary
depending on the bacterial species and the culture conditions (e.g., nutrients,
temperature, and pH) and growth rate. Hydrolytic enzymes such as proteases,
amylases, and celluloses, etc. are produced in the range of milligrams per litre to
grams per litre. Bacteria can grow very quickly, doubling their populations in as
little as every minute. So the retention time, aeration time, efficiency of MBBR
will increase while inoculating external source of Bacteria.

2. Aeration Tank -Activated Sludge Process

The activated sludge of the aeration basin of a wastewater treatment works is a
complex ecosystem of competing organisms. Small molecular weight compounds
diffuse into the bacteria (ingestion) through the cell wall. At the same time, some
larger complex molecules that have been synthesised within the bacteria pass
outwards. This process is referred to as secretion. The secretions include slimes
and gels , that may bond the bacteria together and also enzymes. The enzymes
break down large organic molecules into smaller monomers that are small
enough to be ingested. The bacteria use the ingested molecules for the synthesis
of new molecules, in the process of growth. When they have reached normal size,
the bacterium divides into two, and the process is repeated. If nutrient molecules
are not limiting, this results in exponential growth in the numbers of bacteria. The
bacteria in a wastewater treatment plant comprise both heterotrophs and
autotrophs. The heterotrophic or carbonaceous bacteria are the predominant
group of organisms. They are characterised by feeding mainly on organic carbon
molecules rather than inorganic ones. By contrast, the autotrophs take in
inorganic chemicals, and use these in the synthesis of organic compounds. The
nitrifying bacteria that remove ammonia from the wastewater are the most
important of this group. There are relatively few species of autotrophs, and since
they have low growth rates, they tend to be out-competed by the faster-growing
heterotrophs.
 ACTIVATED SLUDGE PROCESS COLLECTION & EQUALIZATION TANK

Common Effluent Treatment Plants (CETP)

Aerobic Treatment Plants
& Effluent Treatment Plants (ETP)

Anaerobic Digester Sewage Treatment Plants (STP)

Up-Flow Anaerobic Sludge Blankets

MBR (Membrane Bio Reactor)
Reactor (UASBR)

Composting Inoculam Biogas Production In Biogas Digester

Septic Tanks & Sludge Pits Agricultural Biofertilizers

In a well-maintained aeration tank, the bacteria are concentrated in the flocculent

material of the activated sludge, although some always occur free in the
wastewater. The flocs are formed from aggregates of non-living organic polymers
that are probably secreted by bacteria. The bacteria are adsorbed on to the
internal and external surfaces of the floc, and a medium sized floc may harbour
several million bacteria. Immediately after the wastewater enters the aeration
tank, the fine particulates, colloidal particles and large molecules, become
entangled with, and adsorbed to, the floc material. This has the advantage that
the enzymes that are secreted by the bacteria into the water, will tend to be
confined in the vicinity of the substrate, thereby facilitating their digestion.
BACTERIAL FLOC AND ITS METABOLISM

Some goes along the pathway of catabolism or Respiration and ends up as carbon
dioxide. This carbon is lost to the system. The remaining organic carbon follows
the anabolism or Growth pathway and ends up in new biomass. This carbon is
therefore retained in the system. The purpose of respiration is to provide the
energy that is required for growth and for the maintenance of the bacterium.
These three processes – Ingestion, Respiration and Growth - are very highly
coupled or meshed. No one process can go faster than the other. One implication
of this is that, for instance, if you measure the respiration rate, you are indirectly
also measuring the rate of growth and the rate of carbon ingestion

BIOLOGICAL OXYGEN DEMAND

‘Soft’ and ‘Hard’ BOD The time-course for the removal of the organic carbon
varies with the ability of the activated sludge bacteria to ingest it. Small molecular
weight compounds will start to be removed from the sewage immediately after it
has entered the activated sludge tanks. Their removal may be completed in 1 – 2
hours. This group of compounds is often referred to as the readily biodegradable
or ‘Soft’ BOD. Other, higher molecular weight compounds will take several hours
to be degraded and removed. Yet other compounds are more recalcitrant, and
may still be present after several days. This less readily biodegradable BOD is
often referred to as ‘Hard’ BOD.

WHAT HAPPEN WITHOUT AERATION / BLOWER OFF ?

1. Our technology is mainly to reduce the maximum treatment cost by

reduction of usage of number of blowers and blower running time so that
it REDUCES MORE THAN 50 % OF ELECTRICITY USAGE OF STP OPERATION.

2. Instead of giving continuous aeration (physical treatment) to oxidise the

organic and inorganicmatter, our nanozyme selective bacteria’s digest and
degrade the organic and inorganic matters.

3. Our Nanozyme contains more than 60 species of bacteria - this consortium

of aerobic bacteria, anaerobic bacteria, and facultative aerobic bacteria. so while
shutdown or without air, anaerobic bacteria digest and degrade the inert
matters.

MERITS OF ADVANCED BIOLOGICAL TREATMENT TECHNOLOGIES

These include possible mineralization of the dyes to harmless inorganic

compounds like carbon dioxide and water, and formation of a lesser quantity of
relatively harmless sludge. The use of microorganisms for the biodegradation of
dyes is an attractive alternative to the development of bioremediation processes
for the treatment of textile wastewater. Biological methods are environmentally
friendly, produce less sludge than physical and chemical systems, and are
relatively inexpensive, as the running cost is low. Microbial discoloration can
occur via biosorption, enzymatic degradation or a combination of both.

MERITS OF MICROBIAL NANOZYME PRODUCT

Microbial Nanozyme grow in either the presence or absence of oxygen

(Facultative microbes). Non-corrosive, non-pathogenic and low quantities of use,
making it safe and easy to handle and store.

This microbial NANOZYME effectively treats the suspended and floatable organic
debris. Reduces COD (Chemical Oxygen Demand), Reduces BOD (Biological
Oxygen Demand), Reduces TDS – Total Suspended solids, TSS (Total Suspended
Solids) Combination of various strains will successively promotes the growth of
bacterial population, break down and digest the waste in both aerobic and
anaerobic conditions to a far greater degree.

1. Seeding a NANOZYME, in to effluent system reduces the retention time

to a great extent.

2. Specially selected and acclimated strains of microorganisms supplied in

NANOZYME microbial enzyme products produce millions of times the
levels of organic digesting catalysts.

3. Bacteria break down organic matter into simple compounds like carbon
dioxide and water, and they cycle important nutrients such as nitrogen,
sulphur, and phosphorus.
4. The enzymes enable the microbes to use the organic contaminants as
sources of energy and/or as reproductive building blocks.

5. Bacteria can migrate to areas that are rich in specific nutrients that they
require for growth. Bacteria can also attach themselves to surfaces
and form communities known as biofilms.

6. Reduction of Usage of number of blowers and blower time so that it

REDUCES THE MORE THAN 50 % OF ELECTRICITY USAGE OF STP
OPERATION.

7. LOW COST TREATMENT; ONE TIME RECOMMENDED DOSAGE of

application further TRACE AMOUNT based on processing capacity.

8. Reduction of Up to 98% Reduction in BOD & 98% Reduction in COD load,

TDS, Inorganic, organics and heavy metals.

9. Reduction of sludge generation so that reduces the LABOUR COST by

employing SLUDGE HANDLING.

10. Reduction in COLOR AND ODOUR OF THE EFFLUENT.

11.More than 50 % REDUCTION OF USAGE OF CHEMICALS SO that

TREATMENT COST WILL GET REDUCED.
ARE REGULAR MAINTENANCE APPLICATIONS REQUIRED?

1. Usually yes. Just we will boost up with trace dosage. The augmented
microbes may die out or be present in too low a number after a period of
low influent concentration to be effective when the waste strength
increases rapidly.

2. Other conditions (pH, temperature, waste flow rate, alkalinity) will also vary
causing fluctuations in the population dynamics (relative numbers of
different microbes) of the system.

3. For these reasons, regular maintenance application is recommended in

almost every case.

4. Some exceptions are required for start-up assistance and one-time batch
operations. For longer term batch wise treatment re-inoculation is
required as site conditions change.
OUR FEW CLIENTS LIST:
About Dr.Parameswari Ph.D., Agri Enviro.,

Dr.M.PARAMESWARI Technical Director - Ph.D., in Agricultural Science from

TamilNadu Agricultural University with specialization in Industrial Wastewater
treatment heads the operations of the Company.

Dr.M.Parameswari has nearly 17 years experience in university research,

monitoring of industrial effluent treatment plants.

Research Paper Publication -International – 20, National – 16

Awards & Medals: ICAR -2001, University Grant Commission –NET June 2003
&December 2003, Agricultural Scientist Award- 2004 June, UGC PDF- 2014.

Awards:
Publication: The Times Of India - Chennai; Date:2009, May’ 28; Section: Times
City; Page Number: 4

‘I use my knowledge gained during my PhD to recycle effluents’ Parameswari

Shasikumar Page 1 of 1, Publication: The Times Of India - Chennai; Date:2009
May 28;Section:Times City; Page Number: 4 ‘I use my knowledge gained during
my PhD to recycle effluents’

Parameswari Sasikumar opted to be in the field, so she did not take up the
assistant professorship offered to her by the Tamil Nadu Agricultural University. “I
have cleared the national eligibility test required to become an academic, but am
interested in doing service on the industrial side,” says the 29- year-old. She has
done her B Sc Agriculture and M Sc and Ph D in Environmental Science in TNAU.
Her Ph D thesis was on solid and liquid waste management. “I still use the
knowledge I gained during my PhD to develop microbial enzymes to recycle
industrial effluents,” says Parameswari, who has worked in the industrial
consultancy and research department of R V S Institute in Thanjavur and as the
R&D head at J B Nag Water Technologies, a waste treatment company. She says
she has also done a performance audit report for the Pollution Control Board
under former director Dr Rayudu. She is currently working as project manager in
Exnora International and also does consultancy work for water treatment
Our vision
To be globally recognized as the first innovative technology in delivering energy efficient
biological treatment system for waste water treatment.

Our mission
To deliver to all the people of the world the most environmentally responsible,
affordable and effective water treatment systems

Our strategy
To transfer the Innovative Research Technologies to the industrial wastewater
treatment Plants and to Save Energy, the most useful and cost effective waste water treatment
systems

Our values
Innovation is the cornerstone of our business. We place the highest priority on
encouraging, developing, supporting and training our people to be the very best in the
industry Our customers are the ultimate judge of our performance and we strive to be
recognized as being the best in the industry.

Contact Our Technical Team

Dr.M.Parameswari PhD Agri ., Technical Director

Mobile : 9566376690,909500090,Mail: drparams2014@gmail.com,

Mr. B.Rajesh., GM - Technical

Mobile : +91 9484844000, Mail: marketing@bionicsenviro.com

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