Tdeal Chemostatt (modide- v

eneral eq is
I-o tDtA] O Cif Con of compí
A =o at imlet od
understeady state ,

(omp i anteving
to COmp i (in
nput-FF (onxowrcte) i Con" o omp
Output Fa at outet
Rate of
Gi'sappeaance of i om Va (-) Ve-Volume o
Teactos due to TeaxD
Teacto
-i-3 Tate o
Rode o mation
Poltprmatiom = VR Cfi) Cno: o moes ot i
isapaned pes unit

Vo lume) time
.becomes x

Fif F+ Va (-16) - 0
VA(-) - Fif-Fei
r ta-fif E Ci-i
VR
Ve

Ve Vo VR Spau hime
F Vol: lowrate F

E Diluion sate
unit time VR Space veloci ty
unit t i m e-
 Can be wsiten as
AR-DC-CE2
D lurion Tate
tos Constant density s4stem
o mean
D= L Space veloaity
=

Tesiqence time.

Monad Chemostat model

Tox Cellulas teax'S mar qtowth va
Pmax
P- Pmax
S ofo1
ks tS

We know that,
s-D[-CE)
X=
tof Comp
DS x- Xf) toó bioma SS
P =D [P-Pe) tos PToduct
TS = D [s-SE) D Substtate
Tate oenmation o biomass
Tx-dx
dt -
But, P d
Xdt
P X = dx
dt
from and
Px-dx- dx
dt
-
PSpeake qrowth
But 1x =D Lx-xE] Yate

P X DX-Dxf
=

Dxf= D- -

Sterile eed is used, mo cels /biomass present

= O D-)
We krow that, gield z5 -AX

=-0X= ASx ya\s x =

- ASya|s
Px=D[x-MEJ D(-Yas) [s-SE)=

PX = D[s-SJ
- Yals

Px
Yx|s
DISS-PX -0-
Yas
eq'
Kewshng t
monod
usig

PLS-s]- PmaxSx-0
ks+Y
-L
Yels P Tf-x] + Ymax SX
ks +S
Because Yals=- AX
AS

As -AX
Yxs
- Cant be o
Va|s
DD f-XJ+ max
S=0- ks+S
DxF-DX + PmaxSX = 0
ks+S

Dxf+X tmoxS-DJ-o
ks
D+xmoS -D-o ks+S
Monod
chemostat
DS-s-moxS O model
ks+S]Yas eas
Dhen youve Sterile eed , Xf= o and x =XsBene
Xsterile PmaxSsterile
ks+Ssteri le
-= o
PrnaxSshesile -D =O
ks+Ssterile rnax-Dj sslerie = DkS

Pmax
Ssterile =
Dks +Dssterile
S Sterile D ks
Pmax-D

when sterile eed is used

This is the Sushate con
Subshtuting im ,

DSF-S] mox SX O
-

Cks+s)Ys

FmaxS X
Dsf-s =

Cts+ 9s
Yis S s J ks+s=Xsterile
maxS

Xsterle Y\s Sf- ks D

Pmax-D

Ihis is monod chemos tat model.

 Substate Optake and Poduct
omationm

Sbshate Uplake i
without exacellulon dt
Ys x +msX

when Tate expvessed m terms of Spqiouwth vate,

x=PX.
s PX +ms X
als
But PPrmaxS
ks+S

Ys PmaxS +ms |>

s ts)yaJ
S-0 only maintainance.
Witn extracellulas.pct
+P +mg X
Vas YP1s Sp sp 7aBe o
PX+PX tmsX
Ys
P+ms X
ypls
 Geneyal Case when biomass, Subshate and
and pdt
pedt
omation is here m
chemcslat
Vmax max sate
max owth Tte
Pmax F,St
m MM Constant Xi,P do X,s,P
kd dea4th iate.

genesal eq is
dMMo+ Kq-Re
d
Sinae chemostat is Staady state
dM 0
biomass Subshate at
FX FSi FP
FX FP
Mo
pXV
pxv

kdxv
toi BiomasS 9 kd
FXi-FX+PxV-kdxV =0
FCxi-X) =Ptkd VX
Fx-Ki) (-kd] xv
=D = Dx-XiJ=P-kd]x-0
V
his is the
genenal eq"
To stenle cuture, Xi=0 and Pkd sP
DX =PX= D= (kdech
D- tms
kmtS
= Dkm+ DS Pm S
Dkm-Pm-D) S
S Dk
Pn-D
to Substiate 9
fsi-Fs Rc =o
Re =P+ 4 +Ms XV
Ys Ypls
osi-s3 =P +4 +msx
Ys YPs
tos pdt
FPi-Fp +Apx V-o
F P-J 9pXV
D[P-R) = VpX
P= +