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Review
Laser-Based Additive Manufacturing of Magnesium Alloys
for Bone Tissue Engineering Applications: From Chemistry
to Clinic
Mohammad Ghasemian Fard 1 , Fariborz Sharifianjazi 2 , Sanam Sadat Kazemi 3 , Hosein Rostamani 4,5
and Masoud Soroush Bathaei 5,6, *
Abstract: Metallic biomedical implants are made from materials such as stainless steel, titanium,
magnesium, and cobalt-based alloys. As a degradable biometal, magnesium (Mg) and its alloys are
becoming more popular for applications in bone tissue engineering. Mg-based alloys have been found
to be biocompatible, bioabsorbable, and bioactive, allowing them to be used as orthopedic implants
with a low Young’s modulus. Computer-aided design can be used to design scaffolds with intricate
porous structures based on patient-specific anatomical data. These models can be materialized rapidly
Citation: Fard, M.G.; Sharifianjazi, F.;
and with reasonably acceptable dimensional accuracy by additive manufacturing (AM) techniques.
Kazemi, S.S.; Rostamani, H.; Bathaei, It is known that lasers are the most widely investigated energy source for AM’ed Mg, as they offer
M.S. Laser-Based Additive some distinct advantages over other forms of energy. Recent studies have focused on developing
Manufacturing of Magnesium Alloys biodegradable Mg scaffolds by using laser-based AM techniques. In this paper, we aim to review the
for Bone Tissue Engineering recent progress of laser-based AM for Mg alloys and survey challenges in the research and future
Applications: From Chemistry to development of AM’ed Mg scaffolds for clinical applications.
Clinic. J. Manuf. Mater. Process. 2022,
6, 158. https://doi.org/10.3390/ Keywords: magnesium; laser; additive manufacturing; implant; tissue scaffold; bioactivity
jmmp6060158
Figure
Figure 2. 2.
TheThe WE43Mg
WE43 Mgalloy
alloy cubes
cubes in
inthree
threedifferent
differentsizes developed
sizes by Dr.
developed by M.
Dr.S.M.
Bathaei’s groupgroup
S. Bathaei’s
using the LPBF technique for bone tissue engineering applications.
using the LPBF technique for bone tissue engineering applications.
Bone regeneration
Bone regeneration at the site ofatdamage
the site of damage
should beshould be promoted
promoted by successful
by successful implantsimplants
that restore the physical function of bone [30]. In general, they should
that restore the physical function of bone [30]. In general, they should possess character‐ possess characteristics
that are to
istics that are similar similar
thosetoofthose
nativeofbone.
nativeThese
bone. properties
These properties
include: include: (i) the mechanical
(i) the mechanical
properties should be matched to support loading and reduce/minimize the level of stress-
properties should be matched to support loading and reduce/minimize the level of
shielding effect; (ii) biocompatibility, suited for cell adhesion/spreading, and does not
stress‐shielding effect; (ii) biocompatibility, suited for cell adhesion/spreading, and does
release toxic ions to extracellular medium; (iii) an extremely porous structure that facilitates
bone ingrowth and enables robust biological fixation of the implant to the bone [33]. The
natural degradability, biocompatibility, and osteoporotic nature of Mg and its alloys has
led to their rapid development as revolutionary metallic biomaterials in the field of bone
tissue engineering [3–5]. Mg and its alloys have a relatively similar mechanical strength to
that of human bones, as seen in Table 1. Mg and it alloys have high biocompatibility and
similar mechanical properties with natural bone properties that meet two requirements of
implant design intrinsically [7–10]. Moreover, the permeability of the porous metal used
in bone implants is also an important characteristic as a third requirement, since blood
must travel through them in order to transport cells [36]. By utilizing the AM technique
as a new technology, porous Mg-based scaffolds that are tailored to the anatomical needs
of a patient can be manufactured layer-wise using well-developed architectures derived
from computer-aided design (CAD) models in accordance with the patient’s anatomical
requirements [38]. Unique abilities that could not be reached in any way in traditional
Mg production methods can be used, such as die cast [39]. It is also possible in AM
to adjust the modulus of the implant or porous mesh by varying the porosity and strut
size [40]. Among the types of patients who would benefit from AM strategies are those
J. Manuf. Mater. Process. 2022, 6, 158 5 of 18
who require a resection of bone tumors, treatment following fractures, and other types of
bone loss, whether regular or irregular. It has been established that LPBF, formerly known
as selective laser melting (SLM), is the most widely explored method for the AM of Mg and
its alloys [13,14,25], with very few attempts to use direct laser deposition (DLD). Therefore,
DLD was excluded from the main body of the present review.
Figure 4. Mg‐based bone implant manufacture using a typical LPBF technique that uses laser‐based
Figure 4. Mg-based bone implant manufacture using a typical LPBF technique that uses laser-based
AM principle [44].
AM principle [44].
Most
Mostofof the experiments
the experiments onon
thethe
PBFPBFof Mgof alloys
Mg alloys haveconducted
have been been conducted using
using lasers lasers
[50–52].
[50–52]. Theconcentrate
The lasers lasers concentrate high concentrations
high concentrations of heat overofa limited
heat over
areaaoflimited area bed,
the powder of the
powder
meltingbed,
the melting the powder
powder over a limitedover a limited
timeframe timeframe
[53]. [53].solidification
Then, rapid Then, rapid occurs
solidification
as a
result of this short-timed heat flux. It is possible to carry heavier loads due
occurs as a result of this short‐timed heat flux. It is possible to carry heavier loads due to to this rapid
solidification,
this which refines
rapid solidification, the grain
which compared
refines to conventional
the grain compared production methods
to conventional [54,55].
production
The vaporization
methods of some
[54,55]. The elements ofofMg-based
vaporization powderof
some elements occurs when the
Mg‐based powder
powder is heated
occurs when
to a high temperature [56]. In the melt pool, powder vaporization builds
the powder is heated to a high temperature [56]. In the melt pool, powder vaporization up vapor pressure
locallyup
builds [57]. In apressure
vapor melt pool, molten
locally material
[57]. is spattered
In a melt outward
pool, molten by pressure,
material forming
is spattered out‐
low-density structures that may reduce the stress-shielding effect [57,58]. In addition to
ward by pressure, forming low‐density structures that may reduce the stress‐shielding
variations in the chemical composition, this also leads to variations in particle size [59]. For
effect [57,58]. In addition to variations in the chemical composition, this also leads to
printed components to perform well under corrosion conditions, alloying elements must
variations in particle size [59]. For printed components to perform well under corrosion
have good solubility during AM and create corrosion resistance intermetallic phases [59].
conditions, alloying
PBF is influenced in elements must
a significant wayhave
by thegood
power solubility during
of the laser and AM and create
scanning speed incorrosion
order
resistance intermetallic phases [59]. PBF is influenced in a significant way by the power of
the laser and scanning speed in order to determine the melt pool, vaporization, and the
deposition results [60]. There are many factors that affect the quality of depositions, in‐
cluding laser power and scanning speed [61]. However, the effects of each factor on the
J. Manuf. Mater. Process. 2022, 6, 158 6 of 18
to determine the melt pool, vaporization, and the deposition results [60]. There are many
factors that affect the quality of depositions, including laser power and scanning speed [61].
However, the effects of each factor on the other are difficult to describe on their own [60].
J. Manuf. Mater. Process. 2022, 6, 158 In combination, they are essential for determining how much energy is transferred 7 to
of Mg
19
powder via the laser [60]. It has been reported that spherical Mg powder particles with a
purity of 99.9% and a mean size of 24 µm were used with 155.56 J/mm3 energy density
to achieveenergy
decreasing 97.5% density
density[62].
[69].Moreover, researchers
A porous scaffold found that
composed in response
of diamond unittocells
an increase
could
or decrease in energy density, the material’s relative density and
also be constructed with a WE43 alloy. Unit cells with a low energy density of 100 J/mm3 mechanical strength
deteriorated
were [63]. In strut
able to achieve the case of of
sizes Mg400alloy
μmpowders, it was found
[60]. In another study,that
theMg and
laser zinc elements
power was 15
within ZK60 powder were heavily vaporized when subjected to 1250 J/mm 3 of energy
W, and the scanning speed was 20 mm/s, which allowed the Mg‐Al alloy to melt com‐
density
pletely [56]. The
(Figure 5b) melt
[70]. pool
This stabilized
is equivalentandto the vapor
187.5 pressure
J/mm wasenergy.
3 of laser reduced Theafter the laser
Mg–9%Al
density
alloy maywas
havereduced
a rangeto of250 J/mm3 [64].
acceptable energy A poor relative
density regions density
basedof on82.25%
a studywas thesame
of the result
of the incomplete fusion of powder particles. With an energy density of 416.67 J/mm 3,
material at an energy density of 155.6 J/mm3. In addition to powder quality and layer
a maximum relative density of 94.05% could be achieved, as seen in Figure 5a [65]. In
thickness, several parameters affect this range of energy density [71].
another case, with an energy density of 238 J/mm3 , WE43 was printed at a relative density
LPBF of metallic materials must also take porosity into account, which is a common
of 99.4%. WE43 is an alloy of Mg that contains the primary alloying elements yttrium
problem [72]. An analysis of how processing parameters affect the porosity of Mg spe‐
and neodymium [66]. There is evidence suggesting that optimal printing parameters are
cifically has been presented in the following Table 2. Changing any one of the listed pa‐
generally found when the energy density of the melt pool is low, resulting in a high part
rameters will result in a different porosity, so it is difficult to extract a trend based on the
density and low vaporization of the alloying elements inside the melt pool [67]. In addition,
effect of any individual processing parameter [72]. In order to achieve high density and
porosity increases with increasing energy density. The porosity increased from 0.4% to 17%
low porosity, alloy composition dictates the ‘printability’ of the alloy during the opti‐
when the laser power was decreased from 195 W to 135W, and the scanning speed increased
mum processing windows [73]. It is inevitable that porous materials will tear and crack;
from 800 mm/s to 1200 mm/s [68]. In split Hopkinson pressure bar tests, the dynamic
however, it is necessary to avoid hot tearing or cracking while porous materials are pre‐
strength decreased with decreasing energy density [69]. A porous scaffold composed of
sent [74]. Aunit
diamond LPBF component’s
cells could also quality is deteriorated
be constructed with a most
WE43severely
alloy. Unit by hot tearing
cells with aand low
cracking. When the temperature3 gradient remains high, even when
energy density of 100 J/mm were able to achieve strut sizes of 400 µm [60]. In another the constitutional
super‐cooling is lower,
study, the laser power wascolumnar
15 W, grains
and theare more susceptible
scanning speed was to 20 hot
mm/s, tearing
which[75]. Cavities
allowed the
and
Mg-Al alloy to melt completely (Figure 5b) [70]. This is equivalent to 187.5 J/mmtemper‐
hot‐tearing cracks can occur across the full length of columnar grains when 3 of laser
ature andThe
energy. liquid volumealloy
Mg–9%Al fraction
may decrease.
have a rangeSinceof there has been
acceptable limited
energy research
density regions onbased
the
impacts of alloying
on a study of the sameandmaterial
processing
at anparameters
energy densityon hot tearing
of 155.6 J/mmduring LPBF in to
3 . In addition Mg, it is
powder
unclear what effect they have [76].
quality and layer thickness, several parameters affect this range of energy density [71].
Figure 5. (a) Influence of laser scanning speed on relative density of ZK60 [65] and (b) grain size
Figure 5. (a) Influence of laser scanning speed on relative density of ZK60 [65] and (b) grain size
variation of Mg–9%Al powder as a function of laser power and scan speed [70].
variation of Mg–9%Al powder as a function of laser power and scan speed [70].
4. TheLPBF
Advantages of LPBF
of metallic over must
materials Otheralso
AMtake
Processes
porosity into account, which is a common
It is worth
problem [72]. Annoting thatofLPBF
analysis has the added
how processing advantage
parameters affectofthe
being able of
porosity toMgproduce
specifi-
highly porous
cally has been and fine structures
presented as well
in the following as accommodate
Table 2. Changing any many
one offorms, not only
the listed pris‐
parameters
matic ones [72].
will result Thus, it isporosity,
in a different widely soused
it istodifficult
manufacture metallic
to extract scaffolds
a trend based onandtheimplants. In
effect of any
addition,
individualosseointegration studies have
processing parameter [72]. In shown
orderthat implants
to achieve candensity
high be designed
and low with pref‐
porosity,
erentially porous layers
alloy composition to enhance
dictates adhesion of
the ‘printability’ between theduring
the alloy bone tissues and implant
the optimum ma‐
processing
terial [75]. A human bone consists of three distinct anatomical cavities: haversian canals,
osteocytic lacunae, and canaliculi [66–69]. A bone’s mechanical characteristics and pro‐
cesses are remodeled in these three cavities. Living tissues can flourish in porous struc‐
tures, as nutrients can be transported [49–54]. It is possible to design components with a
modulus similar to the bone’s, which reduces stress‐shielding problems in metallic or‐
J. Manuf. Mater. Process. 2022, 6, 158 7 of 18
windows [73]. It is inevitable that porous materials will tear and crack; however, it is
necessary to avoid hot tearing or cracking while porous materials are present [74]. A LPBF
component’s quality is deteriorated most severely by hot tearing and cracking. When the
temperature gradient remains high, even when the constitutional super-cooling is lower,
columnar grains are more susceptible to hot tearing [75]. Cavities and hot-tearing cracks
can occur across the full length of columnar grains when temperature and liquid volume
fraction decrease. Since there has been limited research on the impacts of alloying and
processing parameters on hot tearing during LPBF in Mg, it is unclear what effect they
have [76].
Table 2. Processing-relative densities of LPBF’ed Mg and its alloys for bone tissue engineering applications.
Parameters
Powder Size and Input Energy Relative
Alloys Spot Size Speed Thickness Hatch Ref.
Shape (µm) Power (W) Density (J/mm3 ) Density (%)
(µm) (mm/s) (µm) Spacing (µm)
90 100 300 96.1
Mg Pre-alloyed 43, s 1 90 100 100 30 100 >300 Evaporated
[77]
Mg Pre-alloyed 24, s 70 80 500 30 30 156 97.5 [78]
1250 63 88.2 [79]
195 800 200 41 99.7
195 800 250 33 98.3
WE43 25–63, s 195 100 1200 30 200 27 96.6 [67]
135 1200 200 19 87.6
WE43 25–63, s 200 125 700 30 40 238 99.9 [28]
WE43 25–63, s 200 70 1100 40 130 35 99.7 [80]
WE43 25–63, s 200 90 700 30 40 238 99.8 [81]
120 960 30 104 98.6
WE43 30, p 45, 63, s 150 90 1200 40 104 99.0 [82]
300 1200 208 99.5
GZ151K 25–65, s 200 – 700 30 70 136 97.9 [83]
100 267 98.7
300 89 99.9
500 53 99.7
700 100 38 99.8
GZ112K 31–44, s 80 100 1000 30 27 96.9 [65]
1500 18 71.8
500 50 107 99.5
500 150 36 96.5
G10K 63, s 80 – 200 30 100 133 99.2 [84]
Mg-1Zn Blended Mg-5.5 99.4
Mg-2Zn Zn (36, s), Mg 98.2
Mg-6Zn 180 150 700 20 70 183 94.7 [47]
(31, s) and Zn
Mg-12Zn (19, s) 98.9
6.7 750 94.5
8.3 600 97.4
ZK60 30, s 50 150 10 100 100 500 88.6 [85]
11.7 420 72.8
300 417 94
500 250 93
ZK60 30, s 200 150 700 20 80 179 88 [86]
900 139 84
1 Spherical shape.
structure with such complex external shapes and intricate internal structures is difficult
to fabricate using traditional casting and powder metallurgy techniques. Changing the
specifications of these production techniques may alter the dimensions of the pores, but
it will only produce a porous structure that is arbitrarily arranged [80]. Using LPBF, on
the other hand, can create porous metals with a predefined external form and an inter-
nal structure that complements the rigidity of bone and reduces or diminishes the need
for stress shields during manufacturing [81]. Compared with directed energy deposition
(DED), which is also widely used for the fabrication of metals, PBF has a smaller beam
spot, finer powder, and a thinner layer, which provide better dimensional accuracy [10]. In
addition, the LPBF process is characterized by its high energy density and the absence of
sacrificial binders, which allows for nearly complete densification of metal parts, which is
an advantage over other metal additive manufacturing technologies such as binder jetting.
Laser and electron beam spot sizes are currently between 50 and 100 microns, powder
sizes are between 20 and 50 microns, and the thickness of each powder layer is between
20 and 80 microns for LPBF and electron beam powder bed fusion (EBPBF), respectively.
Therefore, the dimensional deviation between the as-built geometry and the designed
geometry is decreased as a result of the higher forming accuracy of LPBF when compared
to EBPBF [13]. Its high dimensional accuracy, high performance, geometric freedom, and
geometric freedom without rigid support make LPBF suitable for metal implants. There are
many disadvantages to the wire arc additive manufacturing (WAAM) of Mg in comparison
with LBPF, including the possibility of scaffold structures that might be too coarse to be
used in medical applications, the availability of wire (no custom alloys), and the high level
of processing required. Moreover, compared to LPBF, WAAM produces components with
a high deposition rate and surface roughness, resulting in a higher degradation rate of
WAAM’ed Mg implants in physiochemical solutions [14].
such as the AZ91, WE43, and Mg-Gd alloys studied [70]. Thereafter, the grain boundary
becomes brittle and responsible for local failures (e.g., slip and twin transmission across
grain boundaries, grain boundary sliding, etc.). Once this happens, they do not have the
ability to accommodate plastic deformation. It would also be possible for the sputtering
powder or vapor to re-deposit on the surface of the sample, which would result in the part
being poorly consolidated or with a weak bond to the sample [13].
Table 3. Input energy density, grain size, tensile, and electrochemical properties of LPBF’ed Mg and
its alloys.
Mg 97.5 1–5 – – – – 74 3
88.2 – 177 32 [77]
Mg 300 – 52.4 – – – – – [70]
Mg-9Al 250 10–20 70 – – – – – [88]
Mg-9Al 156 1–3 – 274 1 Hank’s solution – – [89]
139 1.6 219 273 3.3
156 1.8 – 233 287 3.1 – –
AZ61 179 2.1 225 261 2.8 [90]
208 2.5 216 239 2.1
120 4.5 70 2.7
140 8 80 – – – – 2.4
AZ61 160 10 93 12 [91]
180 13 90 1.5
AZ91 167 274 296 1.2 – –
83 1–2.9 85–100 237 254 1.8 – – [92]
SBF solution
AZ91 68.6 1–10 115 – – – – – [47]
AZ91 104 1–1.5 – 265 328 3.8 – – [93]
AZ91 278 3.3 – 308 345 1 – – [79]
AZ91-SiC 278 1.1 – 260 300 2 – – [67]
AZ91–2Ca – – – 235 332 3.2 – – [29]
120 34 5.1
WE43 150 27 - - - - 5.0 6–7.2 [28]
300 18 4.4
WE43 238 1 – 296 308 12.2 – – [80]
WE43 35 1–3 – 214 251 2.6 – – [82]
WE43 238 20.4 – – – – – – [82]
G10K 133 27 80 180 228 2.2 0.1 M NaCl – – [83]
J. Manuf. Mater.
GZ151K Process.
136 2022, 6, 158 2 368 3 – – [94] 10 of 1
Mg-1Zn 50 145 11
Mg-2Zn – 46 345 70 2.5 – –
Mg-6Zn 183 65 50 1.5 [95]
Mg-12Zn 83 75 3.2
ZK30 – 80 – – – 17.8 1.23
2000 [96]
ZK30-Cu the sample,
98 which would result in the partSBFbeing solution
poorly47.8
consolidated
2.12 or with a wea
1 Hardness; 2 yield strength; 3 ultimate tensile strength; 4 elongation.
bond to the sample [13].
Figure6.6.Comparison
Figure Comparison of tensile
of tensile properties
properties of Mgofalloys
Mg alloys developed
developed by LPBFby LPBF
and otherand other conventiona
conventional
production techniques
production techniques [87].[87].
Table 3. Input energy density, grain size, tensile, and electrochemical properties of LPBF’ed M
and its alloys.
5.3. Biocompatibility
As AM’ed Mg alloys are the most promising materials for bone tissue implants, there
is a need to consider LPBF’ed Mg alloys’ biocompatibility. The human body requires Mg to
function properly. Due to its biodegradability, Mg is capable of gradually transferring a
load from the implant to the regenerated bone if the implant is biodegradable enough [101].
Mg has a degree of biocompatibility and bioactivity that allows it to promote the prolif-
eration and differentiation of cells even though it is biocompatible [102,103]. In addition
to stabilizing DNA and RNA, it promotes bone growth and healing. Biocompatible alloy-
ing elements are also needed when designing bioabsorbable biomaterials based on Mg
alloys [104]. However, a rapid breakdown of Mg scaffolds provides significant amounts of
hydroxides, Mg ions, and ions from alloying elements to be released in large quantities,
which have an adverse effect on the viability of cells and biocompatibility of the alloy. The
study of cell interactions with biomaterials can be performed by culturing cells [105–107].
LPBF’ed WE43 has been found to be biocompatible in vitro in numerous studies [108].
Four hours after immediate seeding, MG-63 cells in direct contact with WE43 appeared
to be dying as determined by live-dead staining, followed by dual-channel fluorescent
optical imaging (FOI) [109]. After seeding Ti-6Al-4V, on the other hand, no cell death can be
observed after 4 h. It was found that a substantial number of cells survived 24 h after direct
contact with WE43 scaffolds that had been pre-incubated in physiological serum-containing
culture medium for 48 h [110]. Using a similar methodology, it was reported that poor
cellular adherence to the scaffold was observed in a similarly designed study using indirect
extract-based assays (LDH, XTT, and BrdU). RE-based Mg alloys themselves appear to
possess no cytotoxic potential; however, the vast reaction on their bare metal surfaces causes
high levels of hydrogen gas to be evolved and pH shifts local to the surfaces, impairing
the metabolic efficiency of the cells [111]. LPBF’ed WE43 scaffold was found to contain
no viable cells, and only a few dead cells were visible by direct live/dead staining. There
is a possibility of resolving this problem if the surface of the substrate can be modified,
using plasma electrolytic oxidation, which seems to be a suitable niche for adhering cells
when the surface is passivated [112]. Additionally, this technique appears to have reduced
degradation byproduct releases, thus indicating that hardly any signs of cell impairment
were observed. Additionally, there are reports that LPBF scaffolds are manufactured on
pre-alloyed Mg-Nd-Zn-Zr (JDBM) materials, as shown in Figure 7a–i [113]. Similarly, in the
trial of cell adhesion to scaffolds coated with dicalcium phosphate dihydrate vs. scaffolds
left uncoated, more cells adhered to the scaffold coated with dicalcium phosphate dihydrate.
Neither the coated nor uncoated LPBF’ed JDBM scaffolds showed a significant difference in
terms of cytotoxicity in this study: both examples stimulated cell proliferation (Figure 7j–o).
The findings presented in this study and the verification of these findings require a fuller
study and verification, because it is highly unlikely that a direct cell response will not at
least be induced by uncoated AM’ed Mg scaffolds.
Moreover, the presence of bacteria in the implant may cause the implant to loosen or even
fail, demonstrating the importance of inhibiting infections caused by bacteria [114–118]. The
positively charged Cu ions released by the body are capable of attaching to the negatively
charged bacterial cell walls, impairing the permeability of the membranes and eventually
causing the bacterial cell to lyse and die. Additionally, Cu ions can also activate some en-
zymes by interacting with their thiol groups, resulting in bacterial death. Moreover, copper
functions as a cofactor in a wide range of metabolic enzymes in the human body [119].
LPBF’ed ZK60-Cu alloys prepared by Xu et al. exhibited strong antibacterial properties
when they contained Cu [95]. This study indicated that ionized Cu ions, combined with an
alkaline environment, could effectively kill bacteria by damaging the structure of cellular
membranes, causing enzymes to denaturate, inhibiting deoxyribonucleic acid replication
and destroying DNA.
Moreover, copper functions as a cofactor in a wide range of metabolic enzymes i
human body [119]. LPBF’ed ZK60‐Cu alloys prepared by Xu et al. exhibited strong
bacterial properties when they contained Cu [95]. This study indicated that ionize
ions, combined with an alkaline environment, could effectively kill bacteria by dam
J. Manuf. Mater. Process. 2022, 6, 158 12 of 18
the structure of cellular membranes, causing enzymes to denaturate, inhibiting deo
bonucleic acid replication and destroying DNA.
Figure
Figure7. (a–c) Micro-CT
7. (a–c) images,
Micro‐CT (d–f) SEM
images, (d–f)images,
SEM and (g–i) and
images, corresponding magnified images
(g–i) corresponding of
magnified ima
the as-polished B (a,d,g), D (b,e,h), and G (c,f,i) scaffolds. Cytocompatibility of (j–l) the G scaffolds
the as‐polished B (a,d,g), D (b,e,h), and G (c,f,i) scaffolds. Cytocompatibility of (j–l) the G sca
and (m–o) the G-DCPD scaffolds, where (j,m) show the results after 6 h, (k,n) show the results after
and (m–o) the G‐DCPD scaffolds, where (j,m) show the results after 6 h, (k,n) show the result
1 d, and (l,o) show the results after 3 d [113].
1 d, and (l,o) show the results after 3 d [113].
6. Conclusions, Challenges, and Future Perspectives
6. Conclusions, Challenges,
The biocompatibility and Future Perspectives
and biodegradability of Mg alloys make them ideal materials
for biomedical applications. Due to the manufacturing feasibility of complex-shaped
components, it would be possible to fabricate customized Mg components via additive
J. Manuf. Mater. Process. 2022, 6, 158 13 of 18
6.1. Bottlenecks
(1) Laser-based additive manufacturing presents a challenge in terms of producing pre-
alloyed powder. More research is needed in the area of blending magnesium powders
and building consistency.
(2) How the topology of scaffolds affects cell proliferation, new cell growth, and the
lattice structure of the fabricated Mg components that are fabricated using the additive
manufacturing process are still unexplored. This suggests that further studies should
be conducted using in vitro and in vivo methods for the Mg scaffolds manufactured
through LPBF.
(3) Mg implants are evaluated in vivo for their biodegradation performance out of both
processes. Therefore, the study of in vivo processes should be carried out in great
detail in order to succeed in clinical applications. For biomedical implants, LPBF of
Mg components has been shown to be an appropriate and promising alternative. An
alloy that is suitable for bio-implant application could be developed by evaluating
the Mg alloys used currently. An alloying element would be added according to the
strength considerations of the implant in question and its biocompatibility. This will
be considered in future work if a new Mg-based alloy is created.
6.2. Prospects
(1) As a result of the efficient infiltration and complete melting of Mg alloy, LPBF is
a suitable AM technology for the fabrication of Mg implants. This resulted in the
removal of voids and the creation of high-density components. The powder properties
that were used in the manufacturing process of the Mg scaffolds and implants, as well
as the printing parameters used in printing, play a major role in determining their
biological and mechanical properties.
(2) LPBF produces the Mg scaffold with a hierarchical porous structure that mimics the struc-
ture of the human bone in terms of micro- and macro-pores for personalized medicine.
(3) In comparison with other AM techniques, LPBF provides better dimensional accuracy,
because it has a smaller beam spot, finer powder, and a thinner layer. Additionally,
LPBF technology offers high-energy density, no sacrificial binder, and near-complete
densification of metal parts, which makes it superior to other metal additive manufac-
turing processes such as binder jetting and WAAM.
In conclusion, it is expected that future developments of the LPBF process of Mg,
particularly those for bone tissue engineering applications, will focus on a wide range of
properties, including mechanical properties, corrosion performance, biocompatibility, print-
ing properties, biomimetic properties, and biodegradation properties. It would be a reliable
method for building various organs and tissues with diverse mechanical requirements
whether the use of new types of steels as biomaterials with different compositions is being
developed. It would be a reliable way to achieve reprogrammed mechanical properties
and functions. The Mg-based alloys produced by LPBF will be used to make stents, screws,
plates, and scaffolds in the near future.
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