Table I: Mass of Eggs (g) vs Time (minutes)

Sucrose concentration
Time (min) 0% 10% 20% 30% 40% unknown
0 94.7 85.9 85.4 91.6 85.7 90.1
15 99.3 87.0 85.2 91.8 84.6 90.1
30 102.7 87.8 84.7 91.2 83.9 89.7
45 104.3 88.3 84.3 90.0 82.3 89.0

Egg Osmosis: Mass vs. Time

110

105

100

95
Mass (g)

90

85

80

75

70
0 15 30 45 60

Time (min)
75

70
0 15 30 45 60

Time (min)
 Once the data has been entered in Table I, the graph below
will be generated. However, this graph isn't really
informative.

The data in Table I will be manipulated to generate Tables II

and III and the graph on the next worksheet. The isotonic
percentage and the unknown percentage are then
calculated automatically.

ass vs. Time

0% Percent
LinearSucrose
(0%)
10%
Linear (10%)
20%
Linear (20%)
30%
Linear (30%)
40%
Linear (40%)
unknown
Linear (unknown)

60 75
60 75
Table II: Mass Change of Eggs (g) vs Time (minutes)

Mass change (g)

Time (min) 0% suc 10% suc 20% suc 30% suc 40% suc unknown
0
15
30
45
60

Table III: Total Mass Change of Eggs (g) vs Sucrose Concentration (%)
0% 10% 20% 30% 40%
0% suc 10% suc 20% suc 30% suc 40% suc
Tot. Change +9.6 +2.4 -1.1 -1.6 -3.4

Isotonic point plot

Mathematically,
0.0% the equation
0 of the best-fit line is:
Mass Change vs. Percent Sucrose, 0 → 75 min 23.9% 0
23.9% -5
where m is the slope and b is the y-intercept.
+5.0
Rearrange that equation to solve for x (the percent sucrose):
Unknown point plot
0.0% 0.0
+4.0 23.9% 0.0
23.9% percentage,
For the isotonic -5 y = 0.
+3.0
For the unknown percentage, y = calculated mass change for the
unknown.
+2.0

+1.0
Mass change (g)

0.0 slope (m) -30

y-intercept (b) 7.18

-1.0 Isotonic % 23.9%

Unknown % 23.9%
-2.0

-3.0

-4.0

-5.0
0% 10% 20% 33 30% 40%

Percent sucrose