Hot air oven mostwidelyusedsterilizerofdryheatfor

glassware
Uses
Glassware Syringesfetridishes
Surgicalinstrumentlike forcepsscalpels scissors
Oilyfluidswhich are impermeabletowater
Timetemperature pressure
Pressure
1600
for 120min
1700
for 60min
1800
for 40min
Autoclave

Use
sterilize
culturemedia
Rubbergoods

Gowns
Dressing
Time Temp Pressure
121 degrees 15 bslsa.in 15 20minutes
126degrees 2016s 1111 Dominates
133degrees 3016s sa in Bominutes
 Bacterialwaterbath
Uses heatinactivate bacterialvaccines
I hour 560 C

Centrifuge
a techniqueusedforseparationofparticlesfrom a
solution

Inoculatinglook
Use smear streak or takeinoculumfrom
cultural microorganism
of
Pass it at an anglethroughflameof
gasburner
untilentirelengthbecomesorangeofheat
 Sterilecottonswab
Use biologicalsamplecollectiontoavoidsample
contamination

sterilized Autoclave thenhot air oven

Plasticdisposablesyringe
of
Use injection drugs vaccines or
forextraction
ofblood
Sterilization Gamma Radiation EthyleneOxide

Glasssyringe
Use Injectionofdrugs
Sterilization Hot air coven

Microfifette
Use accuratelyandpreciselytransfervolumesof
liquid in microliterrange

GlassSlide
Use prepare smear
Sterilized hot air oven

clarityslide
Demonstratebacterialmotility
byhangingdrop
preparation
method
 Sterilize flaming oven

Peptonewater

Use indoletesting basalmediumforcarbohydrate

fermentation Routineculture
Sterilize Autoclave

Nutrient broth

Use Liquidmediumusedforcultivatingwidevariety
oforganisms Routineculture
sterilization Autoclave 121 15min
 NutrientAgarPlate
Solidsimple

Use Growthof non fastidiousorganism

Sterilization cheatedtodissolveagar then
autoclave
E coli Salmonella S aureus

Blood
agar

Use Growth
of mostaerobic anaerobicbacteria
hemolysis
 Degree of
Differentiates
amongGram coda
Sterilize
Autoclave
for 15min at 121C
5 10 t blood
H influenzae S pneumoniae Neisseria

MacConkey

SelectiveSolid Differential
Allowsgrowth
of gram 0 organism
DifferentiatesLactose fermenting
Enterobacteriae Klebsiella E coli

Chocolate
agar

EnrichedSolidmedia
H
 influenzae h

Robertsoncookedmedia

RobertsonCookedMeat
AnaerobicBacteria Indelicateorganisms

Lowenstein JensenMedia
Mycobacteriaculture
Culture
of M tuberculosis
Gram cocci
staphylococcus Streptococcus
 GramO bacilli
E coli Klebsiella Pseudomonas

Acid fast bacilli

Mtuberculosis

LFcolonies on MacdonkeyAgar

Lactose
Fermenting

Non lactose
fermenting
 ASTplate Mullerhiltonagar

Evaluatesusceptibility
ofa microbe todifferent
antibiotics

Range ofactivityofantibiotic
FactorsaffectingAST
pHofmedium
Thickness of medium
Cultureduration
Moisture

Gram Buddingyeastcell

albicans
IndoleTest

Positive pink orredcolorringinalcoholicdayer

O nocolorchange
Positive E coli
O Enterobacterogen

Methyloud
Positive bright red in colorindicating
acidity
O yellow orangecolor
E coli
Enteroboaterogon

TSI
 Abilityoforganismtofermentglucose lactose or
sucrose
DifferentiatesSalmonella Shigella

CitrateUtilization
Test
Abilityof a organism to use citrate as solesourceof
monoammoniumphosphateas sole source
carbon
of
nitrogen
Org Klebsiella Enterobacter

Bluecolor turbidity citrateutilization

green nogrowth

Ureasetest
Presence
of enzyme urease in bacteria
Proteus Vulgaris
E coli

Gram stain reagent

Primary edge crystalvalet
 iodine mordant
Acetone decolorizes
Counterstaining safranine

ZN stainreagent
Carbolfuschin primary stain
Methylene blue counterstain

BloodcultureBottle

Use Presense bacteria

of or fungi in blood
Autoclave
 DNAmodel

PetriDish
cultivatemicroorganism
Sterilize hot air cover

spiritdamp
Sterilize inoculationlook

Objectivedens compoundmicroscope
of
Present near object used ironing
for
EyePiece
Present near
age viewingofobject

P vivax
Causes Malaria
Sporozoites infective
stage