Neutron NEET 2024 Zoology
Biotechnology: Principles and Processes
PNS – 01
Assertion & Reason
1. Assertion: In a genetic engineering
process, it is necessary to prepare sterile 4.
ambience
Reason: Sterile ambience inhibits the
growth of undesirable microbes during
manufactures of product
[NCERT Page No- 164]
(a) If both assertion and reason are true
and reason is the correct explanation
of assertion.
(b) if both assertion and reason are true
but reason is not the correct
explanation of assertion.
(c) If assertion is true but reason is false
(d) if both assertion and reason are false
2. Assertion: Traditional hybridisation 5.
procedures used in plant and animal
breeding often leads to multiplication of
undesirable genes
Reason: Asexual reproduction preserves
the genetic information while sexual
reproduction permits variations.
[NCERT Page No- 164]
(a) If both assertion and reason are true
and reason is the correct explanation
of assertion.
(b) If both assertion and reason are true
but reason is not the correct
explanation of assertion.
(c) If assertion is true but reason is false 6.
(d) if both assertion and reason are false
3. Assertion: Multiple copies of gene of
interest is synthesized in vivo using PCR
Reason: Taq polymerase remain active
during low temperature
[NCERT Page No- 174]
(a) If both assertion and reason are true
and reason is the correct explanation
of assertion.
(b) If both assertion and reason are true
but reason is not the correct
explanation of assertion.
(c) If assertion is true but reason is false 7.
(d) if both assertion and reason are false
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Assertion: Downstream processing and
quality control testing vary from product
to product
Reason: The process includes separation
and purification of the product
[NCERT Page No- 174]
(a) If both assertion and reason are true
and reason is the correct explanation
of assertion.
(b) If both assertion and reason are true
but reason is not the correct
explanation of assertion.
(c) If assertion is true but reason is false
(d) if both assertion and reason are false
Assertion: Transformation is a process
in which piece of DNA is introduced in
host bacterium
Reason: DNA is hydrophilic molecule
[NCERT Page No- 169]
(a) If both assertion and reason are true
and reason is the correct explanation
of assertion.
(b) If both assertion and reason are true
but reason is not the correct
explanation of assertion.
(c) If assertion is true but reason is false
(d) if both assertion and reason are false
Assertion: Use of chitinase enzyme is
necessary for isolation of DNA from
fungal cell
Reason: Fungal cell wall is made up of
chitin.
(a) If both assertion and reason are true
and reason is the correct explanation
of assertion.
(b) If both assertion and reason are true
but reason is not the correct
explanation of assertion.
(c) If assertion is true but reason is false
(d) if both assertion and reason are false
Assertion: Genetic engineering requires
both nucleases and ligases
Neutron NEET 2024 Zoology
Biotechnology: Principles and Processes
PNS – 01
Reason: Ligases produce the nick in the
recombinant DNA molecule.
(a) If both assertion and reason are true
and reason is the correct explanation
of assertion.
(b) If both assertion and reason are true
but reason is not the correct
explanation of assertion.
(c) If assertion is true but reason is false
(d) if both assertion and reason are false 11.
8. Assertion: Agrobacterium tumefaciens a
pathogen of dicot plants transforms
normal cell into a cancerous cell
Reason: cloning vector have specific
sequences called ‘ori’ region where DNA
replication is initiated
(a) If both assertion and reason are true
and reason is the correct explanation
of assertion.
(b) If both assertion and reason are true
but reason is not the correct
explanation of assertion.
(c) If assertion is true but reason is false 12.
(d) if both assertion and reason are false
9. Assertion: Restriction enzymes
recognize palindromic sequences.
Reason: Palindromic sequences read
same in both directions of the two
strands
[NCERT Page No- 165]
(a) If both assertion and reason are true
and reason is the correct explanation
of assertion.
(b) If both assertion and reason are true
but reason is not the correct
explanation of assertion. 13.
(c) If assertion is true but reason is false
(d) if both assertion and reason are false
10. Assertion: Restriction enzymes are only
endonucleases
Reason: Restriction endonucleases
functions by inspecting a length of DNA
sequence and makes a cut randomly
[NCERT Page No- 166]
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Sankalp
(a) If both assertion and reason are true
and reason is the correct explanation
of assertion.
(b) If both assertion and reason are true
but reason is not the correct
explanation of assertion.
(c) If assertion is true but reason is false
(d) if both assertion and reason are false
Assertion: Restriction enzymes cuts the
strand of DNA and produces sticky ends
Reason: Stickiness of the ends facilitates
the action of the enzyme DNA polymerase
[NCERT Page No- 166]
(a) If both assertion and reason are true
and reason is the correct explanation
of assertion.
(b) If both assertion and reason are true
but reason is not the correct
explanation of assertion.
(c) If assertion is true but reason is false
(d) if both assertion and reason are false
Assertion: DNA fragments move towards
the anode under an electric field.
Reason: Smaller the DNA fragment size
the farther it moves
[NCERT Page No- 168]
(a) If both assertion and reason are true
and reason is the correct explanation
of assertion.
(b) If both assertion and reason are true
but reason is not the correct
explanation of assertion.
(c) If assertion is true but reason is false
(d) if both assertion and reason are false
Assertion: Genetic engineering can
overcome the drawbacks of traditional
hybridization
Reason: Genetic engineering can create
desired DNA sequences to meet specific
requirements
[NCERT Page No- 166]
(a) If both assertion and reason are true
and reason is the correct explanation
of assertion.
Neutron NEET 2024 Zoology
Biotechnology: Principles and Processes
PNS – 01
(b) If both assertion and reason are true
but reason is not the correct 17.
explanation of assertion.
(c) If assertion is true but reason is false
(d) if both assertion and reason are false
14. Assertion: Hind II recognise a specific
sequence of 5 base pairs
Reason: Hind II is the first restriction
exonuclease
[NCERT Page No- 165]
(a) If both assertion and reason are true
and reason is the correct explanation
of assertion.
(b) If both assertion and reason are true
but reason is not the correct
explanation of assertion.
(c) If assertion is true but reason is false
(d) if both assertion and reason are false 18.
15. Assertion: E.Coli having pBR322 with
DNA insert at BamH I site cannot grow in
medium containing tetracycline
Reason: Recognition site for BamHI is
present in tetR region of pBR322
[NCERT Page No- 169]
(a) If both assertion and reason are true
and reason is the correct explanation
of assertion.
(b) If both assertion and reason are true
but reason is not the correct
explanation of assertion.
(c) If assertion is true but reason is false 19.
(d) if both assertion and reason are false
16. Assertion: During gel electrophoresis,
the DNA fragments move towards the
anode
Reason: DNA fragments are created with
the help of restriction endonuclease
[NCERT Page No- 168]
(a) If both assertion and reason are true
and reason is the correct explanation
of assertion.
(b) If both assertion and reason are true
but reason is not the correct
explanation of assertion.
(c) If assertion is true but reason is false
(d) If both assertion and reason are false
#SankalpSelectionKa
Sankalp
Assertion: Insertion of recombinant
DNA within the coding sequence of β-
galactosidase results in colourless
colonies.
Reason: Presence of insert results in
inactivation of β-galactosidase gene
known as insertional inactivation
[NCERT Page No- 170]
(a) If both assertion and reason are true
and reason is the correct explanation
of assertion.
(b) If both assertion and reason are true
but reason is not the correct
explanation of assertion.
(c) If assertion is true but reason is false
(d) if both assertion and reason are false
Assertion: The matrix used in gel
electrophoresis is made up of agarose
Reason: The DNA fragments separate
according to their size through sieving
effect [NCERT Page No- 168]
(a) If both assertion and reason are true
and reason is the correct explanation
of assertion
(b) If both assertion and reason are true
but reason is not the correct
explanation of assertion.
(c) If assertion is true but reason is false
(d) if both assertion and reason are false
Assertion: Plasmids and bacteriophages
have the ability to replicate
independently within bacterial cell
Reason: Some plasmids may have a
high copy number whereas others may
have one or two per cell
[NCERT Page No- 168]
(a) If both assertion and reason are true
and reason is the correct explanation
of assertion.
(b) If both assertion and reason are true
but reason is not the correct
explanation of assertion.
(c) If assertion is true but reason is false
(d) if both assertion and reason are false
Neutron NEET 2024 Zoology
Biotechnology: Principles and Processes
PNS – 01
20. Assertion: Disarmed Ti plasmid can be
utilized as a cloning vector
Reason: A desirable gene is ligated to Ti
plasmid to transfer the desirable DNA
[NCERT Page No- 170]
(a) If both assertion and reason are true
and reason is the correct explanation
of assertion.
(b) If both assertion and reason are true
but reason is not the correct
explanation of assertion.
(c) If assertion is true but reason is false
(d) if both assertion and reason are false
24.
21. Assertion: The separated DNA
fragments can be visualized after
staining the DNA
Reason: PCR is used to amplify the DNA
[NCERT Page No- 168]
(a) If both assertion and reason are true
and reason is the correct explanation
of assertion.
(b) If both assertion and reason are true
but reason is not the correct
explanation of assertion.
(c) If assertion is true but reason is false
(d) if both assertion and reason are false
Sankalp
Reason: Both bacteria and yeast
multiply very fast to from huge
population, which express the desired
gene [NCERT Page No- 164]
(a) If both assertion and reason are true
and reason is the correct explanation
of assertion.
(b) If both assertion and reason are true
but reason is not the correct
explanation of assertion.
(c) If assertion is true but reason is false
(d) if both assertion and reason are false
Assertion: Selection of recombinants
due to inactivation of antibiotics is
cumbersome procedure
Reason: It requires simultaneous
planting on two plates having different
antibiotics [NCERT Page No- 170]
(a) If both assertion and reason are true
and reason is the correct explanation
of assertion.
(b) If both assertion and reason are true
but reason is not the correct
explanation of assertion.
(c) If assertion is true but reason is false
(d) if both assertion and reason are false

22. Assertion: Recombinant DNA is directly
injected into the nucleus of animal cell
Reason: Microinjection is also called as
biolistic or gene gun
[NCERT Page No- 171]
(a) If both assertion and reason are true
and reason is the correct explanation
of assertion.
(b) If both assertion and reason are true
but reason is not the correct
explanation of assertion.
(c) If assertion is true but reason is false
(d) if both assertion and reason are false
25. Assertion: Restriction enzymes belong to
a larger class of enzymes called nuclease.
Reason: ligases are also a type of
restriction endonuclease
[NCERT Page No- 165]
(a) If both assertion and reason are true
and reason is the correct explanation
of assertion.
(b) If both assertion and reason are true
but reason is not the correct
explanation of assertion.
(c) If assertion is true but reason is false
(d) if both assertion and reason are false

23. Assertion: In recombinant DNA

technology, human genes are often
transferred into bacteria (prokaryotes) or
yeast (eukaryotes).

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Neutron NEET 2024 Zoology Sankalp
Biotechnology: Principles and Processes
PNS – 01
Answer Key
S1. Ans. (a) S14. Ans. (d)

S2. Ans. (b) S15. Ans. (a)

S3. Ans. (d) S16. Ans. (b)

S4. Ans. (b) S17. Ans. (a)

S5. Ans. (b) S18. Ans. (b)

S6. Ans. (a) S19. Ans. (b)

S7. Ans. (c) S20. Ans. (a)

S8. Ans. (b) S21. Ans. (b)

S9. Ans. (b) S22. Ans. (c)

S10. Ans. (d) S23. Ans. (a)

S11. Ans. (d) S24. Ans. (a)

S12. Ans. (b) S25. Ans. (c)

S13. Ans. (a)

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