STUOY OF MITDSIS IN ONLON RoOT TIPS

ATM:

Keet Hp to study vaisuA Stages ef nutesis.

RESREMENTS:
On'en bulbs towcal asks lalan ettfes, coeked uial/kahe,
acd,
patidies , sÜSOrS tonceps, Mendles, ntyl aleohot , act a

uoOSLefe Sdes, couexsips 1bletting papen ete

PROCE.DURE
tin oft otd
|: Take a mediun siged bulb of Bnion and
Aetl wattr otts em its bcuse touching Hhe octexby
MeuS
shaxp blade.

tox a uek
toatex with iK base teuching the wctt. kap it
to

Cut5 mn ett the ps o Bots awd put then cito a

3.
w'LtKG o 1:3 acuttl add and mathanf
ual centni ng a
hetueenL :00 am to
nd utuaen 9:30 aun to u: 3o Qm
8:00 Q duug the SummO
duxina uu'nte-)
 GRDWIN ONIDN RooT MPS :
METHÌD oF

ONION BUB

>BoTTLE

’WATER

ONON Root
F
y NA TER
 tha xeuahly
waten

eme. buyduolysd
6 Plaa a deop o aceko Cat mine en a sde: Put

Gteutly sauash the sott by tapping the couaMip uih the

couaNND
i: sep0Mate
bluunt anc of a pencil at nedle unttl the colls
OMd spHLad Gut Unto thin Ray
Mo aut bubbles woe the
Mahe te that thee aHe

Lncake Hhe diding clls. Exanuhe the ditteKent stages o

0BsE RVA TIONS:

Undan lous peuuUA of the miooscope to locate the
CULA. Examine the dittexent stqus nites wnde
aividing taLqes beceMe
dstnct
matwation
Lone

alog
atten

maintaMarc

Proplhase
77 Hetaphae
’Telophaa
> Anaplha
 ntexphase:

(i) Chuomain 4ib26 appaat

uin the nudeus.
() NudeN ENlepe and nucdeolus ane disetnct.

j cheonkin matesíals Ahost ens and cenduses into

aoaad ike stseuCkuMeA caud clseomesemA
at a pgt celed centuo moxe
( ) Nucloas membMANe and nucleolus stats disntegratinn
and disappeax at tha end of pxoplhase

() Abipotax cpindle deuelops

leas
ti) CheoMASemes bacanu aLAnged at the equatox of
spindle.
() kach choWLes OML qet attached to the Spndle 4ibos
at the centOMOHe

the MtOYme nd towands tthe opp escte potes

CeMtXOWMOe
VI Tok amd I 8haps , dupnding upon he poston.
VARIOUs STAGS OF MITDSIS IN ONION RDOT TIP CELLS

’NUCE FAR ME MBRANE

> CHROMA TIN FIBRES

> CEFL MEMBRANE

NUCLE OLUS

Eonly popkae Lott phophose DIS APPEA RING

CELL NALL NUcLEDLUS

’ NUCLEAR. NUELE AR
MEMBRANE
HeNBAANE
>NUCLEoLU S
>CHROOSOMES HRDHOSDMC

mutaph0 sC Staqe
Fosy Anapha
’SPINDLE FIBRES
’ CHROHATDS
’DAUGHHTGR.
CH RoMDSOMES

spIND6
FIBRES
phase
Late Ana
>CELL WAEL
Telopha stage
(HROH 0S0MES > DAUGHTER
CCHROHATIOS)
CELL t DAUGHTER
SpINDLE
PLATE
FlBPES >MUCOEA R
MEM BRANE
 STeloplaaw
) The spahdle dis appaaxA and the daughtex cxohNaSPHEN
untellto Lom chomatin be at Auo potes.
( ) NuLclaaH uMbahL and Lcleous eabpLaMA and Hut

daugltex udet appaass at eppesite potes

( ) CytokKonasis ocuHs by cell plat tonatien buhuan the
Ruo daughtox nLclei

PRECAUTlONS

witth Ltest ofile geing the Heets

the toing betun.

ALame o He spiit launp