Topic Date.

>Am: To deteutne the Cnchie pcenta a âen

pant matental by using îneigiert pasin alyte method
Requihements -’
a) Glas oppan atus Peti dida, sodes, Coves slie, beaker
b)Chentoala SucHOAe Solti,st0ed Wates
PAant Mteia) ’’ Leaves o Rhendiscolan (on, CGrmneliaceae)
d) Miscellaneous, > Qombound minscope
niCAnSCApE PosLep,Needle,bash,
Srapuwatch, qpaph þabos, hlade, latin fabes
Pasncigle+
Active nmetohlsing cell campises cell wal
Semipeamealble membrsane & tonopkt Bulk the
|Space in cell is Gacupied lby yacuale (stosebhnuse o
winera, 0%motic Aubstarces Qrke sugans, anino Qaid
Oqanic acid, seoonday metakoltte OKe
ike Anthocania
betayanin ete)
Under nanaldy fuy ditended candlensed elle ane
placed in AoltianThe movemeat nater
Qccuss fo egíona q high wgtea potential to

Thi iü folued by shtinking acuale awa on

cel ine Plasmelysts Thi steqe when thene' s intfial

Plasnolysad eell can b degaelycd by piocinq then inn

hypctaic sleton In hyprtenîe Aolution tuiqus
pstuue deuelops which prees the
beues themembiue against
 Obgeve'on Tale 7. Plasmelaglg at difBeert Molalities ’
Molalty Total No. No. Paoralyud Peseenkoge Plamyá,
|S.Na
Pignented 9n cells LMeon) ( Mean)

1) Conbol 14 20.33 0.0

21
32 4
2) D.2 5 5.33 14.39%.
21 3
O.3 23 21.69 6 5.33 24.61
21
36 11
34 34.33 10.69 31.06
33 12
23
5) 0.5 25 24.69 11 10,00
26
24 15
6) 0.( 25.64 19
26 13
15.64 61.03%
34
0.4 34 25 72.00/
32 23

’ Table ’ Arnount of watey vls Amount a SuHOe Sohsn(m) tx dt e.’

twates
mobl soludtíon (conc.) Piout o In Sucse tnount of soivt
Cono (0) 10mL L0 mL
.2 m 2 m &ml
O.3m 10 ml
6 mL 10 ml
O.5m 5ml 10m
0,6 m
0,t m L0 mL
 Dato.

Sppaite pteuee oama

’ Wate paterial = OSmutie potertia)+ Paexw loteria
t Mate betcntial

(-) (-)

PHoceausE
0.5 mlal Cm)to im had been plared
2 Epidutmal peeli NeHe talsen eut om hear

Peels ese
G)hey aae AQunted sn saeapeatie salchion ate
o min & bsesed undey mí caçcope
5) Total no. pigemented cells isible in mi sescopee
Count ed
6) Gsaph Nas
Na plated betneen placent a4e cellpla me.
sucOse
coNcantt)on
) Coneentxaion fund nst om qraph at whieh
So cellswere plasmalyped

OSmotie Potential=- CRT baus

C’Moa Aneipiet (So' Ples melysla
R’ Gas canstant
T’ Absolute fn K
Foy given Pant aaia!,
fo
a-CRT
C’ Coneertsatton at which 507. piaalyss Ss=0.5+m
R’Gas ostat .032
T’ Absote tenbesatre +243 =29oK

’,-cRT =o.s)x 082 x29O

=- 13.55 ba = -13.55 xI Rascal =-5. 3+ atm
 Dato

-ve signis giien to Oinctíe potrtfal Qsscin

have lowey wate petential than watex

9) Methcd asumes thak at incipient plasmalyia,

Rom kemhex
nhexatuYE

’ResuLt & Diseuision

Osmotíe þtenhal was tound o be -3.55 bats

Plasmalysls ünat obsewed ta the Catso biled bto

plasimcled aells alie ineaeases bcoause the
ey tenal mediun beeomes mcae hybcatnre hth
irebas tna Cmcens atin with sepent to the
eall iapThi shaws cie the hyefante saln.
moe
mae is the plamelysts
Catinues cyto plaam alm boith
As plasmalyis ontínuen
coll pnérmbiane stant Ahín kina
9ets cucate betaeen eell menbtane &cytoplasm

PAeoattons
1) teels ahaul b ne aelled thick &
tisue & ar bubles
2)Peels shousd be examined attes 7xed dmtíon ime
3) Solutísn shoul be Cauefuy pnehased.
nut be unted
fn plasmaued ceala hey
5) T peeds shorld contan anthcyanin pigment.