QUIRINO STATE UNIVERSITY

DIFFUN CAMPUS
Diffun, 3401 Quirino
COLLEGE OF TEACHER EDUCATION
.... promoting pedagogical excellence

BIOCHEMICAL PROFILING OF FICUS NOTA: BASIS FOR ITS

POTENTIAL APPLICATIONS

An Undergraduate Thesis
Presented to
The Faculty of the College of the Teacher Education
Bachelor of Secondary Education Program
QUIRINO STATE UNIVERSITY
Diffun, Quirino

In Partial Fulfillment of
the Requirements for the Degree of
BACHELOR OF SECONDARY EDUCATION
Field of Specialization: Science

MARY GRACE I. GACUSAN

ARRIANNE S. CAMELLO
JASSER B. RAMIREZ
DERIQ G. CRUZ
June 2023

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 QUIRINO STATE UNIVERSITY
DIFFUN CAMPUS
Diffun, 3401 Quirino
COLLEGE OF TEACHER EDUCATION
.... promoting pedagogical excellence

APPROVAL SHEET
This undergraduate thesis titled: “Biochemical Profiling of Ficus
nota: Basis for its Potential Applications” prepared and submitted by
JASSER B. RAMIREZ, DERIQ MAORENZ G. CRUZ, MARY GRACE I.
GACUSAN, and ARRIANNE S. CAMELLO, in partial fulfillment of the degree
Bachelor of Secondary Education, is hereby endorsed for approval after
undergoing the Oral defense with a final grade of _________________.

DENSON M. LIDAY, M.A.

Thesis Adviser
Date signed: ___________

MA. THERESA B. VALERIO, PhD DIVINA GRACIA S. SABIO,PhD

Member of the Panel Member of the Panel
Date signed: _______________ Date signed: _____________

MARY JOY AGNES B. ALUNDAY, MST

Chair of the Evaluation Committee
Date signed: _________________

Approved and accepted in partial fulfillment of the Degree Bachelor

of Secondary Education Major in Science.

AGATON P. PATTALITAN JR., PhD

Dean, College of Teacher Education
Date signed: _________________

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 QUIRINO STATE UNIVERSITY
DIFFUN CAMPUS
Diffun, 3401 Quirino
COLLEGE OF TEACHER EDUCATION
.... promoting pedagogical excellence

ACKNOWLEDGMENT

” In everything give thanks with the prayer and supplication give praise unto

the Lord. In everything you do, do put God first and He will direct your

path.”

When God works, nothing is impossible. He uses individuals to become a

part of one’s life and be a channel of blessing. Thus, the reason behind a

researcher is thankful to God for He is always at their side throughout the

study.

Likewise, God uses different people to be a part of this undertaking. Thus,

the researchers would like to extend thanks to the following:

Dr. Agaton P. Pattalitan Jr., their dean, for sharing his knowledge;

Mrs. Ma. Olivia Isabella Bautista, their adviser, for her endless guidance and

encouragement to finish this research work;

Dr. Ma. Theresa B. Valerio, their thesis adviser, for supervising the conduct

of this research;

Dr. Denson L. Liday, MAT. for his unending support in checking this paper.

They are very thankful for allowing them to use the laboratory in the College

of Nutrition and Dietetics. He was the inspiration of the researchers in

overcoming technical problems during the conduct of this study;

Their parents and families for their understanding and provisions. They

provided their needs and inspired them to exert more effort to finish this

paper, and to all the uncountable and not written in this writ but

contributed in this paper, to all of you, God bless you more.

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 QUIRINO STATE UNIVERSITY
DIFFUN CAMPUS
Diffun, 3401 Quirino
COLLEGE OF TEACHER EDUCATION
.... promoting pedagogical excellence

DEDICATION

This research is dedicated to our beloved parents for their love and

provisions for all the things we need. We also dedicate this

paper to our alma mater Quirino State University

for its accommodating and passionate way

of developing us. Above all, to our

Father in heaven who gave us

the strength, guidance

and his unending

support.

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 QUIRINO STATE UNIVERSITY
DIFFUN CAMPUS
Diffun, 3401 Quirino
COLLEGE OF TEACHER EDUCATION
.... promoting pedagogical excellence

TABLE OF CONTENTS

PAGE

TITLE PAGE ……………………………………………………………………. i

APPROVAL SHEET ……………………………………………………………. ii

ACKNOWLEDGMENT …………………………………………….…………. iii

DEDICATION ……………………………………………………….................... iv

TABLE OF CONTENTS ………………………………………………………... v

LIST OF TABLES ………………………………………………………………. vii

LIST OF FIGURES ……………………………………………………………... vii

ABSTRACT ……………………………………………………………………… viii

CHAPTERS

I. INTRODUCTION

Rationale ………………………………….……………………………………… 1

Statement of the Problem ……………………………………………………. 4

Scope and Delimitation of the Study ………………………………………… 4

Significance of the Study …………………………………………………….. 4

Conceptual Framework …… ………………………………………………….. 6

Definition of Terms ………………….…….……………………………………. 9

II. LITERATURE REVIEW

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 QUIRINO STATE UNIVERSITY
DIFFUN CAMPUS
Diffun, 3401 Quirino
COLLEGE OF TEACHER EDUCATION
.... promoting pedagogical excellence

Review of Related Literature ……………………….…………………………. 10

Review of Related Studies ………….……………………………………….. 15

III. MATERIALS AND METHOD

Preliminary Processing of the Sample …….……………………………… 21

Biochemical Profiling …………………………………………………………. 21

IV. RESULTS AND DISCUSSION

Percentage Yield ……………………………………………………….……..… 29

Phytochemical Screening …………………………………….………………. 30

Antioxidant Screening Via Total Phenolic Content ………..……….…… 38

Microbial Activity via Disc Diffusion………………..………..……….…..... 38

Potential Applications.………………...………………..………..……….……. 38

V. SUMMARY, CONCLUSION AND RECOMMENDATION

REFERENCES CITED …………………………………………………………. 42

APPENDICES

Documentation………………………………………………………………… 49

Curriculum Vitae…………………………………………………………….. 52

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 QUIRINO STATE UNIVERSITY
DIFFUN CAMPUS
Diffun, 3401 Quirino
COLLEGE OF TEACHER EDUCATION
.... promoting pedagogical excellence

LIST OF TABLES AND FIGURES

Table Page

1 Test outcomes for constituents tested from the given

spray reagents ……………………………………………………. 22

2 Bioactive components of the crude alcoholic extract

of Ficus nota …………………………………………………….... 28

3 Total Phenolic Content ……………………………………..…… 32

4 Result of microbial assay against SA ………………………… 36

Figure

1 Conceptual Framework ……………………………………….. 9

2 TLC spot Test Result ………………………………………….. 28

3 Calibratio Curve of Ascorbic Acid ………………………….. 32

4 Preparation of Standard Calibration Curve ….………….. 33

5 Zone of Inhibition against SA ………………………………… 35

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 QUIRINO STATE UNIVERSITY
DIFFUN CAMPUS
Diffun, 3401 Quirino
COLLEGE OF TEACHER EDUCATION
.... promoting pedagogical excellence

ABSTRACT

Ficus Nota, which belongs to family Moracea, has been use because of

its medicinal value. This research study was conducted to explore its

potential applications in health spectrum to be part of this endeavor. This

experimental research underwent two procedures which were the

preliminary processing of the sample and the biochemical profiling. The

result of the study shows that the alcoholic crude extract of Ficus nota

contained high phenolic content with identification of essential oil and

alkaloids. This means that it has antioxidant property. Therefore, production

of alcoholic beverage and bactericidal alcohol are the potential application

based on the results of the study.

Keywords: Ficus Nota, Phenolic compound, Biochemical Profile, Antimicrobial,

Antioxidant

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QUIRINO STATE UNIVERSITY
DIFFUN CAMPUS
Diffun, 3401 Quirino
COLLEGE OF TEACHER EDUCATION
.... promoting pedagogical excellence

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