THIRD QUARTERLY EXAMINATIONS IN BIOTECHNOLOGY

Name: Score:
Gr. & Section: Date:

I. DNA STRUCTURE. Multiple Choice : Choose and write on the space before the number the letter of the correct
answer.
___ 1. Which of the following statements accurately describes the structure of a nucleotide in DNA?
A. It consists of a sugar-phosphate backbone and a nitrogenous base.
B. It contains two nitrogenous bases bonded together.
C. The sugar component is ribose.
D. The phosphate group is attached to the nitrogenous base.
___ 2. Which of the following is true about the double helix structure of DNA?
A. The two strands are held together by covalent bonds.
B. Adenine always pairs with cytosine, and guanine always pairs with thymine.
C. It was discovered by Watson and Crick in 1953.
D. DNA is a linear molecule without any twisting or folding.
___3. What type of bond holds complementary base pairs together in the DNA double helix?
A. Ionic bonds C. Hydrogen bonds
B. Covalent bonds D. Peptide bonds
___4. Which of the following best describes the structure of the DNA double helix?
A. It consists of two antiparallel strands running in opposite directions.
B. It is a single-stranded structure.
C. It has a random, irregular shape.
D. It contains only three types of nitrogenous bases.
___5. Watson and Crick used the _______ studies of Franklin and Wilkins to construct their model of DNA.
A. Photographic C. X-ray diffraction
B. Electron microscope D. Biochemical
___6. The following is a component of a nucleotide except______
A. Phosphate C. Phospholipid
B. Sugar D. Nitrogenous base
___7. How many nucleotides are there for every turn of a helix?
A. 10 C. 5
B. 20 D.25
___8. Which enzyme binds with the replication fork and separates the two DNA strands?
A. DNA polymerase C. DNA helicase
B. DNA ligase D. DNA gyrase
___ 9. In eukaryotes, Okazaki fragments are consisting of _______ nucleotides long.
A. 100-200 C. 1000-2000
B. 300- 400 d. 3000-4000
___10. DNA replicates itself by semiconservative replication which means
A. each double helix contains an old strand and a newly-synthesized strand.
B. each double helix contains two old strands.
C. each double helix contains two newly- synthesized strands.
D. each double helix does not change in replication.

II. DNA REPLICATION: Word Pair : Choose the correct answer from the given words in the parenthesis.
_________1. ( Leading strand, Lagging strand ) is synthesized continuously during replication.
_________ 2. ( DNA helicase, DNA ligase ) joins the Okazaki fragments on the lagging strand to create a continuous
strand.
_________3. An enzyme which removes RNA primers and replaces them with DNA nucleotides during DNA
replication is ( DNA polymerase I, DNA polymerase II ).
_________4. The process of DNA synthesis is called ( cell division, replication ).
_________5. Nucleotides found in DNA contain the 5- carbon sugar ( deoxyribose, ribose ).
_________6. The backbone of a DNA strand is formed from alternating phosphates and ( sugars, bases ).
_________7. ( Franklin, Chargaff ) analyzed the amounts of A, T, G and C in the DNA of different species.
_________8. Adenine and guanine are ( purine, pyrimidine ) bases.
_________9. DNA Replication takes place during ( First gap phase, Synthesis phase ) of the cell cycle.
_________10. ( 5’- 3’ , 3’-5’ ) is the orientation of the direction of DNA replication.

III. PROTEIN SYNTHESIS: Matching Type: Pair Column B with Column A. Write the letter of the correct answer on the
space provided before the number.
A B

___ 1. The process of making mRNA using DNA as a template a. RNA polymerase
___ 2. The sequence of three mRNA bases b. Promoter
___ 3. It carries specific amino acid to the ribosome during c. Transcription
protein assembly d. Translation
___ 4. It consists of P-site and A-site e. Anticodon
___ 5. Replaces thymine found in DNA f. messenger RNA
___ 6. Formation process of a polypeptide chain at the ribosome g. Genetic code
___ 7. Indicates where transcription is to begin h. transfer RNA
___ 8. The trio of bases in the tag of tRNA i. Codon
___ 9. An enzyme that controls the transcription process j. Uracil
___10. Consists of codons of mRNA that specify a given amino acid. k. ribosomal RNA

IV. Using the Genetic Code. Identify the mRNA codons, tRNA anticodons, and DNA triplets of the hemoglobin:

mRNA codon tRNA anticodon

1. Glycine _______ ________
2. Alanine ________ ________
3. Valine ________ ________
4. Leucine ________ ________
5. Isoleucine _______ ________
6. Phenylalanine _______ ________
7. Tyrosine ________ ________
8. Tryptophan ________ ________
9. Proline ________ ________
10.Serine ________ ________
11. Threonine ________ ________
12.Cysteine ________ ________
13. Methionine ________ ________
14. Aspartic acid ________ ________
15. Glutamic acid ________ ________

Good luck and God bless you all!