Group Leader: Contreras, Christian Alexis L.

Group Members: Mendova, Andrea C.

Santos, Joshua Troy
Suarez, Viva Gia T.
Tolentino, Ma. Elaisa M.
 Introduction
Mosquitoes have always been a danger that people have to deal with. A
mosquito bite can transmit Dengue and it can also transmit the Zika virus,
Chikungunya virus, Malaria virus, Rickettsia infection and other various
parasites and diseases they harbor. Larvicide however can be quite costly for
the remote areas in the Philippines. 20 kilograms of Larvicide can go range
from P7920, if imported from outside the country.
With these facts, the researchers found that citrus fruits such as
Dalanghita are of high medicinal value because of their multiple uses, such
as in the food industry, cosmetics and folk medicine. Dalanghita peel oil
extract which contains a considerable amount of limonene, has numerous
applications including natural insect repellent for use on humans. Their
waste such as their peelings can be used in the production of other by-
products. The researchers would like to prove that the dalanghita peel oil
extract can be economically viable and effective answer to the mosquito
proliferation to prevent another outbreak occurs.
 Statement of the Problem
 What is the mortality rate for the A. aegypti larvae
using Dalanghita peel oil extract after 24 hours? And
48 hours of exposure period?
 Which of the following extract concentrations would
exhibit optimum larvicidal effect? (50% g/mL, 75%
g/mL, 100% g/mL)
 Is the dalanghita peel oil extract effective in being a
larvicidal agent against Aedes aegypti larvae?
 Statement of Hypothesis
Ho: Dalanghita peel oil extracts will
not leave a significant effect as a
larvicide in the 50%, 75%, and
100% extract in all concentrations.
Conceptual Framework
(Schematic Diagram)
 Significance of the Study
 For the people in the Medical Field, this study
will provide these people with basic knowledge and
information as to the potential use of natural
pesticide.
 For the students, this study will provide
information for them to become critical thinkers.
 For the researchers, this study will provide an
open opportunity for the future researchers to
conduct further investigation related to the
problem.
 Scope and Delimitation
of the Study
• This study aims to use the peelings of dalanghita as
an alternative larvicidal agent.
 This study will only focus on the effectiveness of
the dried peelings of dalanghita as a larvicidal. The
researcher does not aim to analyze other possible
outcomes that can be done with the dalanghita
peel.
 Method of Research
Research Design
The study uses an experimental method
of research which includes:
* collection of plant sample
* collection of plant extraction
The result shows proof of the larvicidal
effect Dalanghita peel oil extracts towards
Aedes aegypti larvae.
 Methods of Research
Instrumentation and Validation
 The researchers will use dalanghita fruits that will be
collected from Calamba market and will be
authenticated by the botanist of the Botanical
Herbarium of the University of the Philippines Los
Baños Laguna.
 The Aedes aegypti larvae will be purchased and verified
from the Institute of Biological Sciences of the
University of the Philippines Los Baños Laguna.
 The dalanghita peels will be extracted using Soxhlet
apparatus that are made of Pyrex glass, with high
thermal resistance
 Statistical Analysis
The statistical tools were employed for the
analysis and interpretation of data. The one-way
analysis of variance (ANOVA) is used to determine
whether there are any statistically significant
differences between the means of two or more
independent (unrelated) groups. This will be used
to determine if there is significant difference in
mortality rate among different dalanghita peel
extract concentrations.