ABX Pentra DF 120

Flexible hematology platform 

For internal use only

Prepared by L.Castellane
 ABX Pentra DF 120

• Main features analysis

• Methods and technologies
• Pentra ML

Prepared by L.Castellane
 Main features

Rack loader

Reagent compartment
Technical
software Processed tubes

Stat Sample Bay ABX Pentra ML

Optional slide maker and stainer

Prepared by L.Castellane
 Main features

• CBC, DIFF

• IMmature Lymphocyte, Monocyte and Granulocyte

• Optional and integrated slide maker and stainer

• Expert Validation Station

Prepared by L.Castellane
 Main features

32 Parameters

CBC (12 parameters)

Wbc, Rbc, Hgb, Hct, Mcv, Mch, Mchc, Rdw, Plt, Mpv, Pct, Pdw

Differential count (12+20 parameters)

Lym, Mon, Neu, Eos, Baso, Aly, Lic (% and #)
IML, IMM, IMG (% and #)

Throughput (12O samples per hour)

Prepared by L.Castellane
 Main features

5 reagents
• ABX Diluent
• ABX Basolyse
• ABX Leucodiff
• ABX Alphalyse or ABX Lysebio (Cyanide free)
• ABX Cleaner

Unique blood control

• ABX Difftrol - 3 levels
Target values on disk, multi-language package insert

ABX reagents and controls are bar-coded and CE marked

Batch number, level detection, small reagent volumes, waste level sensor

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 Main features

3 types of analyses (with random access)

• CBC
• DIFF
• SPS

External and internal bar-code reader

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 ABX Pentra DF 120

• Main features analysis

• Methods and technologies • CBC principles
• Pentra ML • 5 Diff – Double matrix

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 Methods & technologies

RBC/PLT - Impedance method

HGB - Cyanmethemoglobin or cyanide free

WBC - (Triangulation)
-> WBC channel (impedance)
-> BASO channel (Specific lyse + impedance)
-> 5Diff channel (optical)

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 Methods & technologies

5Diff Principles 
1. Cytochemistry
Lysing of red blood cells
Staining of nuclei and cytoplasmic granules of leukocytes
Fixing the leukocytes in their original state (48 hours post-draw stability)

2. Double Hydrodynamic Sequential System (DHSS)

Flow cytometer with hydrodynamic focusing before and after the micro-aperture

3. Impedance method
Measurement of the cell volume (“X” axis)  
4. Light absorbance 
Internal structure (“Y” axis)

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 Methods & technologies

Double Diff Matrix

Eosinophils Large Immature Cells

Neutrophils IMmature Granulocytic line

Lymphocytes
IMmature Monocytic line

IMmature Lymphocytic line

Monocytes

Atypical LYmphocytes

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 Methods & technologies

IMmature Granulocytes
Myeloblast

IMG Promyelocyte

Myelocyte

Metamyelocyte

IMG % and # Identification of Granulocytic cell line

count of the overall myelemia

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 Methods & technologies

IMmature Monocyte

Monoblast

IMM Promonocyte

IMM % and # Identification of Monocytic cell line

Count of the precursors of the monocytic line

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 Methods & technologies

IMmature Lymphocyte

Lymphoblast

Prolymphocyte

IML

IML % and # Identification of Lymphocytic cell line

IML and ALY include all abnormal lymphocytic populations

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 Methods & technologies

Routine identification and quantification of 3 immature cell lines involved in leucopoiesis:

• Granulocytic line
• Monocytic line
• Lymphocytic line

-> Reduction of the number of slide examinations

-> Diagnostic and follow-up tool for rapid decision-making

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 Methods & technologies

1- Priority alarms 2- Separation alarms

ALY: (Atypical Lymphocytes) Ne: (Neutro-eosino)

Atypical Lymphocytes Hyper-segmented neutrophils
Plasmocytes, hairy Cells... Hypo-granular eosinophils...

LIC: (Large Immature Cells) Mn: (Mono-neutro)

Blasts, myelocytes Immature granulocytes
Promyelocytes Band cells...
Metamyelocytes...

Nl: (Neutro-lympho)
Hypo-segmented neutrophils
Sezary cells, hairy cells...

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 Methods & technologies

3- Zone alarms No: (Noise)

Platelet aggregates, NRBCs
Lysis resistant RBCs

Ln: (Left-neutro)
Cellular debris

Ll, Ll1: (Left-lympho)

Platelet aggregates, NRBCs

Rn: (Right-neutro)
Immature granulocytes

Rm: (Right-mono)
Large monocytes, blasts

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 Methods & technologies

CHRONIC MYELOID LEUKEMIA

Pentra DF 120 result:

NEU 25.4 %
EOS 1.8 %
LYM 5.1 %
MON 8.6 %
BAS 0.1 %

IMG 49.3 %
IMM 9.2 %
IML 0.5 %
ALY 1.3 %

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 Methods & technologies

ACUTE MONOBLASTIC & MONOCYTIC LEUKEMIA

Pentra DF 120 result:

NEU 11.1 %
EOS 0.5 %
LYM 9.3 %
MON 47.9 %
BAS 0.1 %

IMG 4.1 %
IMM 26.1 %
IML 0.9 %
ALY 1.1 %

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 Methods & technologies

Pentra DF 120 result: T-CELL PROLYMPHOCYTIC LEUKEMIA

NEU 12.1 %
EOS 0.4 %
LYM 6.7 %
MON 12.8 %
BAS 0.4 %

IMG 0.4 %
IMM 2.0 %
IML 7.4 %
ALY 57.8 %

Prepared by L.Castellane
 ABX Pentra DF 120

• Main features analysis

• Methods and technologies
• Pentra ML • Specs & functionality

Prepared by L.Castellane
 Specs & functionality

Dedicated PC
17 inch flat colour screen
Windows XP
CD-ROM burner
Automatic rerun
Reflex testing
Pre-diluted mode
Cytology library
Onboard user manual

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 Specs & functionality

Mono or bi-directional connection:

ASTM format
Transfer of
Demographic data (blood type - man, woman, child)
Tests requested (CBC, CBC/DIFF, SPS)

Up to 3 ABX Hematology analyzers (Pentra range) can be connected

Query mode
Download capability

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 Specs & functionality

User defined rules applied when :

• receiving the worklist,

• a sample has been analyzed.

_ Rerun capability
_ Reflex testing
_ Automatic validation
_ Messages, comments….

Bio-technical validation with :

Patient history
Delta check
Result edition
Manual entry
Recalculation capability
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 Result screens

Bio-technical validation with :

• Patient report, run, re-run, patient history, graphs, alarms. All displayed on the
same screen.
• Delta check
• Result edition
• Manual entry
• Recalculation capability

1- Title bar
2- Patient and Graphics area
3- Messages
4- Patient Comments
5- Technical Comments
6- Extended Diff
7- Result area
8- Report
9- Runs

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 Specs & functionality

Hematovision IV included
A cell cytology library

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 ABX Pentra 120 series

Prepared by L.Castellane
 ABX Pentra 120 series

Optional Double Validation

CBC / DIFF RET NRBC's
SPS DIFF Matrix station
ABX Pentra 120  
ABX Pentra 120 Retic   
ABX Pentra DF 120    
ABX Pentra DX 120      

Prepared by L.Castellane
 Thank you

Prepared by L.Castellane