Progress Report

Adviser: Da-Jeng, Yao

Reporter: Min-I, Liao

Date: 2020/09/22
Outline
• Tensile test
• Blood staining
• The protocol of cell marketed
• CTCs recognize
• Immunotherapy Case
• Future work
Tensile test

3mm

Velocity Max force

Sample 1 0.01mm/s 0.7N
Sample 2 0.001mm/s 0.8N
Sample 3 0.001mm/s 3.2N
 Protocol of cell marketed
• Decide which fluorophore you need
before you buy antibody

• Defferent fluorophore need defferent

Light source
light source to excite

PE
 CTCs recognize

Epcam CD45 Hoechst

Single CTCs + - +
CTCs in WBCs cluster + +(cluster) +
CTM with CD45+ cluster +(>1) +(cluster) +
CTM with CD45- cluster +(>1) - +

• The problem I faced:

colorant target
• The CD45’s wavelength is too close Epcam cancer cell
with Hoechst’s so they will be excited CD45 leukocyte
together Hoechest nucleus
Immunotherapy Case – 黃 OO

2019/09/20 2019/09/20
18
8%
16
14

CTC number (per 2ml)

12
Single CTCs 10
24%
CTCs in WBCs cluster 8 17
CTM with CD45+ 6
cluster
4
CTM with CD45- cluster 6
2
2 0
0
Single CTCs CTCs in WBCs CTM with CD45+ CTM with CD45-
cluster cluster cluster
68%
type
Future work
• SACA chip test
• Learn how to make SU8 film
• Continue to do the blood stain
TH A NK S